CN1796543A - Biological cell tissue microdissection device - Google Patents

Abstract

The present invention provides a biological cell tissue micro-cutting device, which includes: a platform and a vibrating cutting part. The platform can provide a slide with a biological cell tissue for placement, and can perform displacement movement in a space. The vibrating cutting part also includes a fine needle and a vibrating body, the fine needle is connected to the vibrating body, and the vibrating body can provide the fine needle with energy required for a vibration, so that the fine needle performs a cutting action on the biological cell tissue. The platform can move the biological cell tissue through a three-axis displacement movement, so that the vibrating cutting part can cut the biological cell tissue at any position.

Description

Biological cell tissue microdissection device

technical field

The present invention relates to a biological cell tissue micro-cutting device, in particular to a biological cell tissue micro-cutting device that can perform three-axis movement in space and detect the depth of the cutting position.

Background technique

With the rapid development of molecular biotechnology and the increasing frequency of application of this technology to the study of human diseases, today's bioscience community is at the beginning of a revolution in precision biomedicine. For example, in the field of tumor research, slicing tumor tissue from the perspective of molecular biomedicine to analyze ribonucleic acid (RNA) or deoxyribonucleic acid (DNA) in the tissue plays an important role. This is because the general cell tissue is usually composed of a variety of cells mixed with complex biochemical and physical characteristics, and the suspected diseased area is not completely composed of problem cells.

In addition, the more you study the gene expression of problematic cells or specific cell tissues, the most ideal is a homogeneous cell population. However, the procedure for extracting a homogeneous cell population is not easy, and the correctness of the analysis results is often disturbed by the mixing of cells from other neighboring populations. Therefore, it is necessary and urgent to effectively identify and obtain technical research and development of diseased tissue blocks.

In recent years, due to the laser micro-beam micro-dissection technology such as laser capture micro-dissection (Laser Capture Micro-dissection, LCM), laser micro-dissection (Laser Micro-Dissection, LMD) and laser pressure capture device (Laser Pressure Catapulting, LPC ) etc. have developed micro-dissection that can provide fast, high selectivity and low hybridity, so the method of micro-dissection of cell tissue by laser technology is still the mainstream of micro-dissection design of cell tissue. However, the above three micro-dissection methods of cell tissue may be effective from the perspective of "cutting", but the unfortunate and unavoidable disadvantages are "radiation contamination of samples", "too expensive" and their functions are limited to cutting. question. In other words, if it is desired to identify questionable or suspected cancerous tissue by biopsy, it is necessary to develop a low-cost cell tissue extraction and cutting system with minimal adverse side effects . In view of this, in order to solve the above problems, there is an urgent need for a biological cell tissue micro-dissection device to meet the actual needs.

Contents of the invention

The technical problem to be solved by the present invention is to provide a biological cell tissue micro-cutting device, which uses a platform capable of displacement movement in space and a vibrating cutting part to achieve the purpose of controlling the movement of biological cell tissue to any position and cutting.

The secondary object of the present invention is to provide a biological cell tissue micro-cutting device, which can cut tissue by using fine needles with optional longitudinal vibration and optional lateral vibration.

Another object of the present invention is to provide a biological cell tissue micro-cutting device, which can detect the cutting position of the fine needle by using a distance sensor arranged on the fine needle.

In order to achieve the above object, the present invention provides a biological cell tissue micro-cutting device, which includes: a platform and a vibrating cutting part. The platform can provide an object slide with a biological cell tissue, and the platform can perform at least two-axis displacement movement in a space. The vibrating cutting part can carry out one-axis displacement movement in space, and the vibrating cutting part also includes a thin needle and a vibrating body, the thin needle is connected with the vibrating body, and the vibrating body can provide the fine needle for The energy required by the vibration makes the fine needle perform cutting action on the biological cell tissue. Wherein, through the platform capable of at least two-axis displacement movement and the vibrating cutting part capable of one-axis movement, a three-dimensional displacement movement is formed in space, so that the biological cell tissue can be moved, and the vibrating cutting part can be aligned at any position. The biological cell tissue is cut.

In order to achieve the above object, the present invention also provides a biological cell tissue micro-cutting device, which includes: a platform and a vibrating cutting part. The platform can provide an object slide with a biological cell tissue, and the platform can perform three-axis displacement movement in a space. The vibrating cutting part also includes a fine needle and a vibrating body, the fine needle is connected with the vibrating body, and the vibrating body can provide the energy required for the vibrating of the fine needle, so that the fine needle can conduct the biological cell tissue cutting action. Wherein the platform can move the biological cell tissue through three-axis displacement movement, so that the vibrating cutting part can cut the biological cell tissue at any position.

In order to achieve the above object, the present invention also provides a biological cell tissue micro-cutting device, which includes: a platform and a vibrating cutting part. The platform can provide an object slide with a biological cell tissue, and the platform can perform at least one axis of displacement movement in a space. The vibrating cutting part can carry out two-axis displacement movement in space. The vibrating cutting part also includes a fine needle and a vibrating body. The fine needle is connected with the vibrating body. The vibrating system can provide the fine needle for The energy required by the vibration makes the fine needle perform cutting action on the biological cell tissue. Wherein, through the platform capable of at least one axis of displacement movement and the vibration cutting part capable of two axes of movement, a three-dimensional displacement movement is formed in space, so that the biological cell tissue can be moved, and the vibration cutting part can be aligned at any position. The biological cell tissue is cut.

The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but not as a limitation of the present invention.

Description of drawings

Fig. 1 is the schematic diagram of the first preferred embodiment of the biological cell tissue micro-cutting device of the present invention;

Fig. 2 is the schematic diagram of the second preferred embodiment of the biological cell tissue micro-cutting device of the present invention;

Fig. 3 is the schematic diagram of the third preferred embodiment of the biological cell tissue micro-dissection device of the present invention;

4A to 4D are schematic diagrams of other preferred embodiments of the biological cell tissue microdissection device of the present invention.

Among them, reference signs:

1- Biological cell tissue micro-dissection device

11-Platform

12- Vibration cutting department

121 - fine needle

122-vibrator

13-Optical observation device

14-bracket

15-slide

16-sensor

Biological cell tissue microdissection device

21-Platform

22- Vibration cutting department

121 - fine needle

122-vibrator

23 - Optical Observation Device

24-bracket

25-slide

26-sensor

Biological cell tissue microdissection device

31-Platform

32- Vibration cutting department

321 - fine needle

322-vibrator

33 - Optical Observation Device

34-bracket

35-slide

36-sensor

4- biological cell tissue

81 - longitudinal vibration

82-Lateral Vibration

91- First degree of freedom displacement motion

92-Second degree of freedom displacement motion

93-Third degree of freedom displacement motion

Detailed ways

Please refer to FIG. 1 , which is a schematic diagram of a first preferred embodiment of the biological cell tissue microdissection device of the present invention. The biological cell tissue micro-cutting device 1 includes a platform 11 and a vibrating cutting part 12 . The platform 11 can provide a slide 15 with a biological cell tissue 4 placed on it, and the platform 11 can perform two-axis 91 , 92 displacement movement in a space. The vibrating cutting part 12 can perform a displacement movement of an axis 93 in the space. The vibrating cutting part 12 also includes a thin needle 121 and a vibrating body 122 , the thin needle 121 is connected with the vibrating body 122 , and the thin needle 121 can be made of glass, hard metal such as steel or tungsten.

The vibrating body 122 can provide the energy required for the fine needle 121 to perform ultrasonic vibration, so that the fine needle 121 can be selected to generate a longitudinal vibration 81 and a lateral vibration 82 can be selected to make the fine needle 121, so as to facilitate the biological cell tissue 4 Perform cutting action. In this embodiment, the vibrating body 122 can be selected as a piezoelectric material. The biological cell tissue microdissection device 1 also has a support 14 , an optical observation part 13 and a sensor 16 . The bracket 14 can be used to fix the vibrating cutting part 12 , and the optical observation part 13 can be used to observe the state of the fine needle 121 cutting the biological cell tissue 4 . In order to precisely control the position of the fine needle 121 , the sensor 16 can detect the position of the fine needle 121 during the cutting process. The sensor 16 can be one of a vibration sensor and an acoustic wave sensor.

Please refer to FIG. 2 , which is a schematic diagram of a second preferred embodiment of the biological cell tissue microdissection device of the present invention. The biological cell tissue micro-cutting device 2 includes a platform 21 and a vibrating cutting part 22 . The platform 21 can provide a slide 25 with a biological cell tissue 4 placed on it, and the platform 21 can perform three-axis 91 , 92 , 93 displacement movements in a space. The vibrating cutting part 22 also includes a thin needle 221 and a vibrating body 222, the thin needle 221 is connected with the vibrating body 222, and the thin needle 221 can be made of glass material, hard metal such as: steel or tungsten .

The vibrating body 222 can provide the energy required for the fine needle 221 to perform ultrasonic vibration, so that the fine needle 221 can be selected to generate a longitudinal vibration 81 and a lateral vibration 82 can be selected to make the fine needle 221, so as to facilitate the biological cell tissue 4 Perform cutting action. In this embodiment, the vibrating body 222 can be selected as a piezoelectric material. The biological cell tissue microdissection device 2 further has a support 24 , an optical observation part 23 and a sensor 26 . The bracket 24 can be used to fix the vibrating cutting part 22 , and the optical observation part 23 can be used to observe the state of the fine needle 221 cutting the biological cell tissue 4 . In order to precisely control the position of the fine needle 221 , the sensor 26 can detect the position of the fine needle 221 during the cutting process. The sensor 26 can be one of a vibration sensor and an acoustic wave sensor.

Please refer to FIG. 3 , which is a schematic diagram of a third preferred embodiment of the biological cell tissue microdissection device of the present invention. The biological cell tissue micro-cutting device 3 includes a platform 31 and a vibrating cutting part 32 . The platform 31 can provide a slide 35 with a biological cell tissue 4 placed on it, and the platform 31 can perform a displacement movement of an axis 91 in a space. The vibrating cutting part can perform two-axis 92, 93 displacement movement in the space. The vibrating cutting part 32 also includes a thin needle 321 and a vibrating body 322 , the thin needle 321 is connected with the vibrating body 322 , and the thin needle 321 can be made of glass, hard metal such as steel or tungsten.

The vibrating body 322 can provide the fine needle 321 with the energy required for ultrasonic vibration, so that the fine needle 321 can be selected to generate a longitudinal vibration 81 and a lateral vibration 82 can be selected to make the fine needle 321, so as to facilitate the biological cell tissue 4 Perform cutting action. In this embodiment, the vibrating body 322 can be selected as a piezoelectric material. The biological cell tissue microdissection device 3 also has a support 34 , an optical observation part 33 and a sensor 36 . The bracket 34 can be used to fix the vibrating cutting part 32 , and the optical observation part 33 can be used to observe the state of the fine needle 321 cutting the biological cell tissue 4 . In order to precisely control the position of the fine needle 321 , the sensor 36 can detect the position of the fine needle 321 during the cutting process. The sensor 36 can be one of a vibration sensor and an acoustic wave sensor.

Please refer to FIG. 4A to FIG. 4D , which are schematic diagrams of other preferred embodiments of the biological cell tissue microdissection device of the present invention. Inheriting the spirit of the above invention, in order to achieve the three-axis 91, 92, 93 movement in space, under the spirit of the invention, the arrangement and combination of the platform and the displacement movement of the vibration cutting part are all within the scope of the spirit of the invention.

Next, the operation method of the device of the present invention will be described, with FIG. 1 as an illustration icon, the combination of the vibrating cutting part 12 and the platform 11 can accomplish the goal of micro-dissection of biological cell tissue. The lateral vibration 82 or longitudinal vibration 81 of the thin needle 121 can be achieved by the predetermined force intensity, amplitude and frequency of the vibrating body 122 . The tip of the fine needle 121 will contact the surface of the tissue to be cut in a planned way, and cause the tissue block to be destroyed, so as to achieve the purpose of tissue cutting. The biological cell tissue 4 is placed on the platform 11, and the tip of the fine needle 121 is controlled to any position of the tissue through the two-axis movement 91, 92 of the platform 11 and the one-axis 93 movement of the vibrating cutting part 12, and the vibrating body 122 vibrates the fine needle 121 to achieve the effect of microcutting the biological cell tissue 4 .

There are two cutting methods. The first method is to make the thin needle 121 vibrate longitudinally through the vibrating body 122, and perform acupuncture cutting along the boundary of the area where the biological cell tissue 4 is to be cut. In this cutting method, the vertical vibration frequency of the fine needle should be as high as possible, and the horizontal vibration should be avoided as much as possible.

The second method is to make the thin needle 121 vibrate in the lateral direction through the vibrating body 122 . The biggest difference between this method and the above method is that the thin needle 121 does not cut the tissue in a straight line. Instead, the area to be cut in the biological cell tissue 4 will be scraped off piece by piece by the fine needle oscillating transversely. If there are some small blocks connected to the tissue, compared with the above method, this method can separate the small tumor from the tissue faster by cutting the small tumor tissue.

If the tissue cutting device adopts the first method to cut the tissue, the desired area to be cut can be completely cut off, and can be moved out to the observation area through the suction device or the cannula. If the second method is adopted, the liquid covering the tissue block will be sucked out together with the fragments separated from the tissue block, and then collected for testing or research. Generally, the tissue block is usually very thin, so in most practical examples, if the first method is used, the vibration amplitude of the thin needle 121 only needs to be operated in the micron range.

In order to achieve the purpose of cutting, the tip of the fine needle 121 must penetrate into the interior of the biological cell tissue 4 as much as possible; Based on this, in order to provide the best cutting position and depth of the thin needle during the tissue cutting process. The present invention adds a sensor 16 to solve the above problems. The sensor 16 can be one of a vibration sensor and an acoustic wave sensor. Taking the vibration sensor as an example, the vibration sensor is assembled on the tissue cutting device. When performing tissue cutting, the needle tip needs to be inserted into the tissue until it lightly touches the slide 15, and then the fine needle is lifted up until the tissue is cut. The vibration sensor shows that the fine needle 121 is in empty vibration. After this preliminary arrangement, the operator can get an optimal cutting position and depth. Another feasible method is to install the acoustic wave sensor in the area near the platform supporting the tissue mass, and record the contact between the fine needle and the slide, and then adjust the penetration depth of the fine needle appropriately to obtain the best cutting depth.

Based on the above, the present invention can meet the demands of the industry and improve the competitiveness of the industry due to the characteristics of easy cutting, simple manufacturing and easy assembly.

Certainly, the present invention also can have other multiple embodiments, without departing from the spirit and essence of the present invention, those skilled in the art can make various corresponding changes and deformations according to the present invention, but these corresponding Changes and deformations should belong to the scope of protection of the appended claims of the present invention.

Claims (11)

1, a kind of device for micro cutting tissue of biological cells is characterized in that, includes:

One platform, it can provide puts the microscope slide with a biological cell tissue, and this platform can carry out three displacement movement in a space; And

One vibration cutting part, it also includes a fine needle and a vibrating mass, and this fine needle is connected with this vibrating mass, and this vibrating mass can a vibrational frequency provide this fine needle to vibrate required energy, makes this fine needle carry out cutting action to this tissue of biological cells.

2, a kind of device for micro cutting tissue of biological cells is characterized in that, includes:

One platform, it can provide to put has one of biological cell tissue microscope slide, and this platform can carry out the displacement movement of diaxon at least in a space; And

One vibration cutting part, it can carry out a motion in the space, this vibration cutting part also includes a fine needle and a vibrating mass, this fine needle is connected with this vibrating mass, this vibrating mass can a vibrational frequency provide this fine needle to vibrate required energy, makes this fine needle carry out cutting action to this tissue of biological cells.

3, a kind of device for micro cutting tissue of biological cells is characterized in that, includes:

One platform, it can provide to put has one of biological cell tissue microscope slide, and this platform can carry out at least one displacement movement in a space; And

One vibration cutting part, it can carry out two axle movement in the space, this vibration cutting part also includes a fine needle and a vibrating mass, this fine needle is connected with this vibrating mass, this vibrating mass can a vibrational frequency provide this fine needle to vibrate required energy, makes this fine needle carry out cutting action to this tissue of biological cells.

As claim 1,2 or 3 described device for micro cutting tissue of biological cells, it is characterized in that 4, this vibrational frequency is the ultrasound frequency.

5, as claim 1,2 or 3 described device for micro cutting tissue of biological cells, it is characterized in that, this vibration can select a longitudinal vibration and a transverse vibration one of them.

6, as claim 1,2 or 3 described device for micro cutting tissue of biological cells, it is characterized in that, this fine needle may be selected to be a tungsten material, steel and a glass material one of them.

As claim 1,2 or 3 described device for micro cutting tissue of biological cells, it is characterized in that 7, this vibrating mass is a piezoelectric element.

8, as claim 1,2 or 3 described device for micro cutting tissue of biological cells, it is characterized in that, also include a transmitter, it can provide the position of this fine needle of detecting in cutting process.

9, device for micro cutting tissue of biological cells as claimed in claim 8 is characterized in that, this transmitter, can select a sonic sensor and a vibration transducer one of them.

10, as claim 1,2 or 3 described device for micro cutting tissue of biological cells, it is characterized in that also having an optical observation portion, provide this fine needle of observation to cut the state of this tissue of biological cells.

As claim 1,2 or 3 described device for micro cutting tissue of biological cells, it is characterized in that 11, this fine needle can be replaced by other biological processing instrument.

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