CN1785054A - Health-care food having anti-oxidation function and its prepn. method - Google Patents
Health-care food having anti-oxidation function and its prepn. method Download PDFInfo
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Abstract
A health-care food with antioxidizing function is prepared from the extract of grape seed and the extract of ganoderma. Its preparing process is also disclosed.
Description
Technical field
The invention belongs to field of health care food, relate to a kind of health food, the invention still further relates to the preparation technology of this health food with anti-oxidation function.
Background technology
Grape seed extract is rich in OPC.OPC
[1]Be extensively to be present in the botanic natural polyphenol compound that belongs to the biflavone derivative; this compounds is that the flavanols by different numbers is polymerized; heating generates OPC in acid solution; has extremely strong anti-oxidation characteristics; generally be present in the skin of fruit and the wooden real part of plant, it mainly acts on is the composition of easy oxidation in the protective plant.As far back as the fifties French scientist just find from pine bark, to extract a large amount of OPCs, can contain 85% OPC in its extract.Then find that grape pip is to extract the better resource of OPC the seventies, Proanthocyanidin from Grape-seed Extracts can be up to 95%.
The eighties, the radical pair health affected was familiar with by people day by day, and modern medicine and nourishing healthy are learned and thought that free radical can directly cause numerous disease in human body, and be also relevant with the generation of other diseases.French scientist studies show that one of the strongest effective free radical scavenger, especially its activity in vivo that the grape seed extract OPC is up to now to be found, other antioxidant is incomparable especially.Bagchi.D once carried out testing in the body with mouse; use the grape seed extract OPC; VC; VE; the liver that beta carotene is induced TPA; the protective effect of lipid peroxidation compares in the brain tissue; discovery is the grape seed extract OPC under 100mg/kg.BW dosage; VC; VE; beta carotene all can reduce the active oxygen that TPA induces generation; use peritoneal macrophage luminescence method reduced rate to be respectively 70%; 18%; 47% and 16%; use cytochromes reducing process reduced rate to be respectively 65%; 15%; 37% and 19%; find also that simultaneously the grape seed extract OPC for suppressing the peritoneal macrophage oxygen production that TPA induces, has dose-response relationship.The result shows that the grape seed extract OPC can play a protective role to oxidative damage, and its protection is better than other antioxidant.In addition, the in vitro test of Bagchi.D has also obtained similar result.The author uses chemoluminescence method and cromoci reducing process to measure the grape seed extract OPC respectively, VC, VE, beta carotene, superoxide dismutase, the removing ultra-oxygen anion free radical of catalase and sweet mellow wine and the ability of hydroxyl radical free radical, the result shows, the grape seed extract OPC has the concentration-response relation to the inhibition of free radical, when the concentration of 100mg/L, grape seed extract is 78% and 81% to the inhibition ability of ultra-oxygen anion free radical and hydroxyl radical free radical, under the equal conditions, the ability that VC suppresses above-mentioned two kinds of free radicals is 12% and 19%, VE is 50% and 75%, the ability that superoxide dismutase and catalase synergy suppress super oxide anion is about 83%, and the ability of sweet mellow wine inhibition hydroxyl radical free radical is 87%, illustrate that the grape seed extract OPC is a kind of VC of ratio, the free radical scavenger that VE is stronger has antioxidation.In addition, grape seed extract also has protection cardiovascular and prevention hypertension, antitumor, radioresistance, anti-sudden change, skin care and beauty treatment, antiallergy and anti-inflammatory, improves effect such as visual performance
[2]
At present abroad, the application that grape pip extracts OPC is very extensive, its disease-prevention health effect basis is exactly its ability of removing free radical, in addition, it also has bioavilability preferably, is easy to combine with collagen stabilizing cell membrane and antienzyme activity, these functions and oxidation resistance are collaborative, have become a kind of health products that have much DEVELOPMENT PROSPECT.
Glossy ganoderma (Ganoderma lucidum) is called red sesame.Glossy ganoderma is a kind of macro fungi, belongs to Ganodermataceae, and its dry fructification is used as medicine, and glossy ganoderma is used as medicine and is stated from Shennong's Herbal the earliest, is traditional tonic, and successive dynasties book on Chinese herbal medicine ancient books and records all have record.Glossy ganoderma is warm in nature flat nontoxic, have cardiac stimulant, improve the coronary flow circulation, remove blood fat, reduce MCO, strengthen the effects such as tolerance of cardiac muscle the body anoxic, and Ganodenna Lucidum P.E can effectively improve the heart, brain tissue superoxide dismutase (SOD) activity, reduce lipid peroxide (LPO) and lipofuscin content, have significantly anti-oxidant, Green Tea Extract, the effect of antagonism peroxide injury
[3]
At present still do not adopt grape seed extract and Ganodenna Lucidum P.E prescription to prepare the health food of anti-oxidation function.
List of references:
1, state plants, Xu Li, OPC, the autonomic drug with broad development prospect.Foreign medical science (autonomic drug fascicle), 1996,11 (1): 196.
2, Zhao Chaoying, Yao Xiaoman, the alimentary health-care function of grape seed extract OPC (summary), Chinese food health magazine, 2000,12 (6), 38-41.
3, Zhang Xiangpei, Song Jiajin etc., glossy ganoderma is to the protective effect of rat peroxide injury.Qufu Normal University's journal: natural science edition, 2002,28 (1), 79-80.
Summary of the invention
The health food that the purpose of this invention is to provide a kind of antioxidation.
A further object of the invention provides the preparation method of this health food.
Theoretical foundation of the present invention is: grape seed extract has very strong antioxidant characteristics, Ganodenna Lucidum P.E can improve the activity of body superoxide dismutase, promptly has indirect antioxidation activity, and have simultaneously and improve blood circulation, lipopenicillinase, enhancing cardiac muscle tolerance the body anoxic, glossy ganoderma and grape seed extract are with using, can strengthen antioxidation, have many benefits that glossy ganoderma brings to body simultaneously concurrently.We have antioxidation.
The objective of the invention is to realize by following technical measures:
A kind of health food with antioxidation, its prescription contains the raw material of following weight portion:
Grape seed extract 70-120 part, Ganodenna Lucidum P.E 60-110 part.
Described health food, wherein grape seed extract is directly bought from market and is obtained, its quality standard is: OPC 〉=90.0%, moisture≤5.0%, ash content≤5.0%, heavy metal≤10.0ppm, total number of bacteria≤1000cfu/g, mould and yeast≤100cfu/g, no coliform, no salmonella.The outward appearance of grape seed extract is the rufous powder.
Described health food, wherein Ganodenna Lucidum P.E prepares by following method: glossy ganoderma adds 8-12 times of water gaging after cutting into slices, extract 2-3 time, each 4-6 hour, merge extract, concentrate the back spray-drying; The quality requirement of Ganodenna Lucidum P.E is: polysaccharide 〉=10.0%, moisture≤8.0%, ash content≤10.0%.The outward appearance of Ganodenna Lucidum P.E is the dark-brown powder, drying, and free from admixture has the intrinsic fragrance of glossy ganoderma.
Health food with antioxidation of the present invention can add any one auxiliary material that pharmaceutically allows, its preparation can be any one formulation that pharmaceutically allows, include but not limited to tablet, granule, capsule, oral liquid, syrup, pill etc., can also add the health food that other materials that do not weaken health food anti-oxidation function of the present invention are made complex function in the health food of the present invention.
The preparation technology of described health food comprises the following step:
Get Ganodenna Lucidum P.E and grape seed extract mixes by prescription, common process is made required preparation.
Beneficial effect of the present invention:
The pharmacological experimental data of health food of the present invention
One, anti-oxidation function animal experiment report
(1) materials and methods
1, given the test agent: the grape curing capsule, the 0.18g/ grain, Nanjing Zhongke Biochemical Technology Co., Ltd provides, lot number: 20040518.(by the embodiment of the invention 1 preparation, down together)
2, experimental animal: 12-14 monthly age SD male and healthy rat is provided by west, Shanghai pul-Bi Kai animal used as test Co., Ltd.
3, reagent: superoxide dismutase (SOD), lipid peroxide (MDA), glutathione peroxidase (GSH-Px), lipofuscin kit build up bio-engineering research by Nanjing and are provided.
4, key instrument: T-1000 type electronic balance, 722 type spectrophotometers, RF-5000 type sepectrophotofluorometer (day island proper Tianjin)
5, dosage grouping: grape curing capsule human body recommended intake is 360mg/ people/day (pressing the 60kg batheroom scale), be equivalent to 6mg/kgbw, press respectively 5 times of human body recommended intakes, 10 times, 30 times designs low (30mg/kgbw), in (60mg/kgbw), high (180mg/kgbw) three dosage groups, other establishes control group (0mg/kgbw), replaces sample with sterilized water.Sample is prepared with sterilized water, and basic, normal, high dose concentration is respectively 3mg/ml, 6mg/ml, 18mg/ml, and per os gives the thing that tried of rat respective concentration once a day, presses 10ml/kgbw and irritates stomach, surveys every anti-oxidant index after continuous 30 days.
6, test method: the anti-oxidation function method of inspection by " health food check and assessment technique standard (200 years versions) " is carried out.
Rat is bought the back Animal House endoadaptation environment 5 days, gets blood through eye socket, measures lipid peroxide in its serum (MDA) content.According to MDA content in the rat blood serum, be divided into 4 groups at random, 10 every group, grouping according to dosage requires to be respectively blank group, basic, normal, high dosage group.Test group gives the grape curing capsule of corresponding dosage, and the blank group replaces given the test agent with sterilized water, irritates stomach every day, continuous 30 days, weighs weekly.Measure lipofuscin content in Content of MDA, SOD vigor, GSH-Px vigor, the hepatic tissue after 30 days.
7, raising condition: rat is that 18-22 ℃, relative humidity are to raise in the barrier system of 40%-70% in temperature.
8, data analysis: with SPSS10.0 software each experiment initial data is carried out homogeneity test of variance, satisfy the neat data information that requires of variance and carry out statistical disposition with the comparative approach in twos of mean between a plurality of experimental group in the one-way analysis of variance method and control group; The data information of Non-Gaussian Distribution or heterogeneity of variance is carried out suitable variable conversion, wait to satisfy normal distribution or variance are neat require after, carry out statistical disposition with the data of changing gained.
(2) result:
1, the grape curing capsule is to the influence of rat body weight
The initial body weight of rat is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, comparative approach in twos with mean between a plurality of experimental group in the one-way analysis of variance method and control group carries out statistical disposition, as shown in Table 1, basic, normal, high three dosage groups are compared with control group, and there are no significant for difference (P＞0.05).The initial body weight that is rat is comparatively balanced between each group.
Per os gives the grape curing capsule 30 days of rat various dose, each dosage group rat body weight of being measured is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, comparative approach in twos with mean between a plurality of experimental group in the one-way analysis of variance method and control group carries out statistical disposition, as shown in Table 1, basic, normal, high three dosage groups are compared with control group, and there are no significant for difference (P＞0.05).
Table 1 grape curing capsule is to the influence of rat body weight (n=10, x ± SD)
| Group | Starting weight (g) | P | The 1st week (g) | The 2nd week (g) | The 3rd week (g) | The 4th week (g) | P |
| Control group | 577±54 | 586±48 | 593±46 | 601±53 | 599±64 | ||
| Low dose group | 593±49 | 0.852 | 587±40 | 590±47 | 603±50 | 607±47 | 0.975 |
| Middle dosage group | 628±56 | 0.134 | 597±44 | 610±39 | 615±42 | 618±43 | 0.764 |
| High dose group | 631±69 | 0.103 | 596±64 | 613±55 | 618±53 | 608±56 | 0.967 |
2, the grape curing capsule is to the influence of lipid peroxide in the rat serum (MDA) content
By table 2 as seen, MDA content compares between basic, normal, high three dosage groups and control group group in the preceding rat serum of experiment, and there are no significant for difference (P＞0.05).Promptly comparatively balanced between each group of MDA content in the rat serum before the experiment.
Per os gives the grape curing capsule 30 days of rat various dose, MDA content in each dosage group rat serum of being measured is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, comparative approach in twos with mean between a plurality of experimental group in the one-way analysis of variance method and control group carries out statistical disposition, as shown in Table 2, MDA content is compared with control group in basic, normal, high three the dosage group rat serum in experiment back, and there are no significant for difference (P＞0.05).
Table 2 grape curing capsule is to the influence of MDA content in the rat serum (n=10, x ± SD)
| Group | MDA (nmol/ml) before the experiment | P | Experiment back MDA (nmol/ml) | P |
| Control group | 3.53±0.44 | 4.61±0.79 | ||
| Low dose group | 3.54±0.47 | 1.000 | 4.03±1.02 | 0.481 |
| Middle dosage group | 3.52±0.50 | 1.000 | 4.34±1.38 | 0.898 |
| High dose group | 3.49±0.52 | 0.996 | 3.56±0.95 | 0.085 |
3, the grape curing capsule is to the influence of superoxide dismutase in the rat serum (SOD) vigor
Per os gives the grape curing capsule 30 days of rat various dose, SOD vigor in each dosage group rat serum of being measured is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, comparative approach in twos with mean between a plurality of experimental group in the one-way analysis of variance method and control group carries out statistical disposition, as shown in Table 3, the SOD vigor is significantly higher than control group in basic, normal, high three dosage group rat serum.
Table 3 grape curing capsule is to the influence of SOD vigor in the rat serum (n=10, x ± SD)
| Group | SOD in serum (U/ml) | P |
| Control group | 373±87 | |
| Low dose group | 557±63 | 0.000 |
| Middle dosage group | 538±114 | 0.001 |
| High dose group | 599±105 | 0.000 |
4, the grape curing capsule is to the influence of glutathione peroxidase in the rat serum (GSH-Px) vigor
Per os gives the grape curing capsule 30 days of rat various dose, GSH-Px vigor in each dosage group rat serum of being measured is carried out normal state, homogeneity test of variance, satisfy the normal distribution requirement, comparative approach in twos with mean between a plurality of experimental group in the one-way analysis of variance method and control group carries out statistical disposition, as shown in Table 4, the GSH-Px vigor is significantly higher than control group in basic, normal, high three dosage group rat serum.
Table 4 grape curing capsule is to the influence of GSH-Px vigor in the rat serum (n=10, x ± SD)
| Group | Serum GSH-Px (unit of activity) | P |
| Control group | 359±79 | |
| Low dose group | 408±24 | 0.051 |
| Middle dosage group | 419±24 | 0.012 |
| High dose group | 428±24 | 0.004 |
5, the grape curing capsule is to the influence of lipofuscin content in the rat liver
Per os gives the grape curing capsule 30 days of rat various dose, lipofuscin content in each dosage group rat liver of being measured is carried out homogeneity test of variance, satisfy the homogeneity of variance requirement, comparative approach in twos with mean between a plurality of experimental group in the one-way analysis of variance method and control group carries out statistical disposition, as shown in Table 5, lipofuscin content significantly is lower than control group in basic, normal, high three dosage group rat livers.
Table 5 grape curing capsule is to the influence of lipofuscin content in the rat liver (n=10, x ± SD)
| Group | Lipofuscin (μ g/g tissue) | P |
| Control group | 3.49±0.61 | |
| Low dose group | 3.22±0.50 | 0.519 |
| Middle dosage group | 2.89±0.47 | 0.033 |
| High dose group | 2.90±0.44 | 0.038 |
Conclusion: the grape curing capsule gives SD old rats with basic, normal, high three dosage groups (being respectively 30mg/kgbw, 60mg/kgbw, 180mg/kgbw), behind the continuous irrigation stomach 30 days, lipofuscin content in MDA content, SOD vigor, GSH-Px vigor, the hepatic tissue in the mensuration rat blood serum.The result shows that the grape curing capsule can improve SOD and GSH-Px vigor in the rat serum, reduces lipofuscin content in the liver tissues of rats, has antioxidation.
Two, grape curing capsule anti-oxidation function human experiment experiment
(1) material and method
1, given the test agent: the grape curing capsule, the 0.18g/ grain, 100/bottle, Nanjing Zhongke Biochemical Technology Co., Ltd provides, lot number: 20040518.The human body recommended intake is everyone every day 2 times, each 1.
2, the experimenter includes standard in: select age 45-65 year, health states is good, does not have obvious brain, the heart, liver, lung, kidney, blood illness, does not have long-term clothes medication history, and aspiration is tried to guarantee the crowd that cooperates.
3, get rid of experimenter's standard:
3.1, gestation or women breast-feeding their children, to the health food allergy sufferers;
3.2, merge to have the inclination, serious disease patients such as liver, kidney and hemopoietic system;
3.3, take the article relevant in a short time with being tried function, have influence on judgement person to the result;
3.4, do not meet the standard of including in, edible in accordance with regulations given the test agent, can't judge effect or data not umbra ring effect or security judgement person.
4, study subject grouping:
Large hospital carried out human experimentation jointly during Nanjing Medical University health analyzing and testing center and Southeast China University were attached.The experimenter is divided into test-meal group and control group at random by MDA, SOD, GSH-Px level, considers influence result's principal element such as age, sex, living and diet custom etc. as far as possible, carry out harmony to check, with the comparativity between the assurance group.Every group of experimenter is no less than 50 examples.
5, test-meal method: the former life of experimenter during being tried, diet are constant.The test-meal group is taken the grape curing capsule, and control group is taken comfort Liu reference substance, and every experimenter eats 2 times every day, each 1, takes continuously 3 months.
(2) observation index:
1, security is observed
1.1 ordinary circumstance: spirit, sleep, diet, stool and urine, blood pressure.
1.2 blood urine is routine inspection just.
1.3 biochemical indicator is measured: liver, renal function (total serum protein TP, albumin A LB, glutamic-pyruvic transaminase ALT, glutamic-oxalacetic transaminease AST, urea nitrogen BUN, creatinine Cr).
1.4 Chest X-rays, electrocardiogram, Abdominal B type ultrasonography inspection.
2, effect index:
2.1 lipid peroxide content: variation and the MDA decline percentage of observing test-meal front and back MDA;
2.2 superoxide dismutase: variation and the SOD rising percentage of observing test-meal front and back SOD;
2.3 glutathione peroxidase: variation and the GSH-Px rising percentage of observing test-meal front and back GSH-Px.
3, data are handled with the result and are judged:
3.1 data are handled: the own control data adopts paired t-test, two groups of means relatively adopt t check in groups, the latter need carry out homogeneity test of variance, the data of Non-Gaussian Distribution or heterogeneity of variance are carried out suitable variable conversion, after waiting to satisfy the normal state homogeneity of variance, carry out the t check with data converted; If translation data still can not satisfy the requirement of normal state homogeneity of variance, use t ' check or rank test instead; But the coefficient of variation too data of big (as CV＞50%) is used rank test.Comparing difference does not have under the prerequisite of conspicuousness between group before test, can test between the group of back and compare.
3.2 the result judges: between group statistical significance is arranged more all after each effect observation index test-meal front and back self comparison and the test-meal, can judge this index positive.Each experimental result positive in MDA, SOD, three experiments of GSH-Px, this given the test agent of decidable has the effect of anti-oxidation function.
(3) result
1, test-meal group and control group are relatively harmonious
Experimenter's 100 examples of all information, each 50 example of test-meal group and control group, the male sex 34 people wherein, women 66 people.Age 45-65 year, 51.4 years old mean age.By table 6 as seen, MDA, SOD, GSH-Px, age and control group compare before the test-meal of test-meal group, and no significant difference (P＞0.05) shows that two groups of test-meals preceding MDA, SOD, GSH-Px, age, sex have balanced comparativity.
Relatively harmonious before table 6 test-meal
| Index | Test-meal group (n=50) | Control group (n=50) | The P value |
| MDA(mmol/L) | 3.63±1.64 | 3.59±1.37 | 0.884 |
| SOD(mmol/L) | 94.22±21.04 | 93.10±17.71 | 0.773 |
| GSH-Px | 113.32±39.55 | 115.92±47.26 | 0.767 |
| Age (year) | 51.56±4.60 | 51.16±5.25 | 0.686 |
| Sex (man/woman) | 18/32 | 16/34 | 0.833 |
2, test-meal group and control group ordinary circumstance are relatively
Examination trencherman spirit, sleep, diet situation have been carried out the interrogation investigation, press, generally, differential levels adds up.By table 7 as seen, test-meal grape curing capsule experimenter ordinary circumstance is good, and test-meal group, control group sleep quality, diet situation all are significantly improved before and after the test-meal.
Ordinary circumstance relatively before and after table 7 test-meal
| Test-meal group (n=50) | Control group (n=50) | |||||||||||||
| Before the test-meal | After the test-meal | P | Before the test-meal | After the test-meal | P | |||||||||
| Good | Generally | Difference | Good | Generally | Difference | Good | Generally | Difference | Good | Generally | Difference | |||
| Spirit | 44 | 6 | 0 | 47 | 3 | 0 | 0.083 | 46 | 4 | 0 | 47 | 3 | 0 | 0.317 |
| Sleep | 39 | 11 | 0 | 49 | 1 | 0 | 0.002 | 33 | 16 | 1 | 46 | 4 | 0 | 0.000 |
| Diet | 45 | 5 | 0 | 49 | 1 | 0 | 0.046 | 44 | 6 | 0 | 48 | 2 | 0 | 0.046 |
| Stool and urine | 49 | 1 | 0 | 50 | 0 | 0 | 0.317 | 48 | 2 | 0 | 49 | 1 | 0 | 0.317 |
3, to blood, urine, the just influence of safety index such as routine and biochemistry
As shown in Table 8, before and after the test-meal group, control group test-meal every hematology and liver, renal function index, blood pressure all in normal range (NR), difference that relatively there are no significant before and after the test.Urine, stool routine examination, Chest X-rays, electrocardiogram, Abdominal B type ultrasonography show no obvious abnormalities before and after test-meal group and the control group test-meal.Show that test-meal grape curing capsule is not to human body blood, urine, just routine and liver, renal function, blood pressure, cardiopulmonary, liver spleen etc. produce harmful effect.
Two groups of every safety indexes change (n=50) before and after table 8 test-meal
| Before the test-meal | After the test-meal | |||||
| The test-meal group | Control group | P | The test-meal group | Control group | P | |
| Leucocyte (10 9/L) | 5.35±1.14 | 5.31±1.32 | 0.874 | 6.13±1.96 | 5.75±1.42 | 0.267 |
| Red blood cell (10 12/L) | 4.56±0.43 | 4.69±0.46 | 0.165 | 4.51±0.40 | 4.38±0.35 | 0.080 |
| Hemoglobin (g/L) | 140.16±13.43 | 145.32±13.05 | 0.054 | 134.44±7.66 | 132.00±8.77 | 0.142 |
| Blood platelet (10 9/L) | 163.42±56.76 | 178.36±59.80 | 0.203 | 205.64±28.08 | 192.92±49.83 | 0.119 |
| Total protein (g/L) | 73.52±3.26 | 72.29±4.68 | 0.129 | 72.33±3.98 | 70.93±5.24 | 0.137 |
| Albumin (g/L) | 45.60±1.59 | 45.58±1.87 | 0.954 | 45.13±2.79 | 46.18±5.36 | 0.222 |
| ALT(U/L) | 23.72±12.29 | 22.32±15.71 | 0.621 | 18.26±9.23 | 17.30±6.93 | 0.558 |
| AST(U/L) | 24.18±8.64 | 22.76±12.97 | 0.521 | 30.48±7.04 | 28.00±7.46 | 0.091 |
| Urea (mmol/L) | 5.34±1.34 | 5.14±1.27 | 0.446 | 5.66±1.43 | 5.61±1.11 | 0.844 |
| Creatinine (umol/L) | 57.52±13.79 | 58.86±11.50 | 0.599 | 56.00±11.75 | 57.18±15.61 | 0.670 |
| Systolic pressure (mmHg) | 109.7±6.1 | 110.0±8.9 | 0.835 | 109.6±6.6 | 110.6±8.9 | 0.551 |
| Diastolic pressure (mmHg) | 70.1±5.2 | 70.7±7.4 | 0.638 | 70.0±5.4 | 70.8±7.4 | 0.579 |
| Urine is conventional | Normally | Normally | Normally | Normally | ||
| Stool routine examination | Normally | Normally | Normally | Normally | ||
| Chest X-rays | Normally | Normally | Normally | Normally | ||
| Electrocardiogram | Normally | Normally | Normally | Normally | ||
| Abdominal B type ultrasonography | Normally | Normally | Normally | Normally |
4, the grape curing capsule is to the influence of serum lipid peroxide (MDA)
As shown in Table 9, the test-meal group is compared the Content of MDA there was no significant difference before the test-meal with control group.Test-meal group Content of MDA significantly is lower than control group after the test-meal.Test-meal group, control group test-meal front and back self paired comparisons Content of MDA there was no significant difference.Drop-out value there was no significant difference before and after test-meal group, the control group test-meal.
Table 9 grape curing capsule is to the influence (n=50) of serum lipid peroxide (MDA)
| Group | Before the test-meal | P | After the test-meal | P | Drop-out value | P | P 1 |
| The test-meal group | 3.63±1.64 | 0.884 | 3.24±0.53 | 0.000 | 0.39±1.72 | 0.077 | 0.118 |
| Control group | 3.59±1.37 | 3.78±0.80 | -0.19±1.49 | 0.375 |
Annotate: P
1Be test-meal front and back self paired comparisons, following table together.
5, the grape curing capsule is to the influence of serum superoxide dismutases (SOD)
As shown in Table 10, the test-meal group is compared SOD in serum content there was no significant difference before the test-meal with control group.Test-meal group SOD in serum content is significantly higher than control group after the test-meal.Test-meal group test-meal front and back self paired comparisons has significant difference; Control group test-meal front and back self paired comparisons there was no significant difference.Lift-off value has significant difference before and after test-meal group and the control group test-meal, and the test-meal group percentage that on average raises is 29.09%.
Table 10 grape curing capsule is to the influence (n=50) of serum superoxide dismutases (SOD)
| Group | Before the test-meal | P | After the test-meal | P | Lift-off value | P | P 1 |
| The test-meal group | 94.22±21.04 | 0.773 | 121.63±12.11 | 0.000 | 27.41±24.01 | 0.000 | 0.000 |
| Control group | 93.10±17.71 | 90.25±23.66 | -2.85±27.91 | 0.474 |
6, the grape curing capsule is to the influence of serum glutathione peroxidase (GSH-Px)
As shown in Table 11, the test-meal group is compared serum GSH-Px content there was no significant difference before the test-meal with control group.Test-meal group serum GSH-Px content is significantly higher than control group after the test-meal.Test-meal group test-meal front and back self paired comparisons GSH-Px content has significant difference; Control group test-meal front and back self paired comparisons GSH-Px content there was no significant difference.Lift-off value has significant difference before and after test-meal group and the control group test-meal, and the test-meal group percentage that on average raises is 14.56%.
Table 10 grape curing capsule is to the influence (n=50) of serum glutathione peroxidase (GSH-Px)
| Group | Before the test-meal | P | After the test-meal | P | Lift-off value | P | P 1 |
| The test-meal group | 113.32±39.55 | 0.767 | 129.82±27.79 | 0.000 | 16.50±27.86 | 0.000 | 0.000 |
| Control group | 115.92±47.26 | 106.33±29.19 | -9.58±37.78 | 0.079 |
Conclusion:
1, examination trencherman ordinary circumstance before and after the test-meal grape curing capsule, blood pressure, blood, urine,, just Chest X-rays before and after routine and liver, kidney, biochemical function, the test-meal, electrocardiogram, Abdominal B type ultrasonography inspection show no obvious abnormalities, and show that test-meal grape curing capsule do not see harmful effect to human body.
2, the effect observed result of test-meal grape curing capsule shows: self compares before and after the test-meal of test-meal group, SOD in serum, GSH-Px significantly raise, the rising percentage is respectively 29.09%, 14.56%, compare between test-meal group and control group group after the test-meal, serum MDA, SOD, GSH-Px content have significant difference.Show that the grape curing capsule has anti-oxidation function.
The specific embodiment
The invention will be further elaborated by the following examples.
Raw material sources that embodiment adopts and quality standard explanation:
Grape seed extract is directly bought from market and is obtained, and its quality standard is: OPC 〉=90.0%, moisture≤5.0%, ash content≤5.0%, heavy metal≤10.0ppm, total number of bacteria≤1000cfu/g, mould and yeast≤100cfu/g, no coliform, no salmonella.The outward appearance of grape seed extract is the rufous powder.
Ganodenna Lucidum P.E prepares by following method: glossy ganoderma adds 8-12 times of water gaging after cutting into slices, extract 2-3 time, and each 4-6 hour, merge extract, concentrate the back spray-drying; The quality requirement of Ganodenna Lucidum P.E is: polysaccharide 〉=10.0%, moisture≤8.0%, ash content≤10.0%.The outward appearance of Ganodenna Lucidum P.E is the dark-brown powder, drying, and free from admixture has the intrinsic fragrance of glossy ganoderma.
Embodiment 1
Get grape seed extract 99g, Ganodenna Lucidum P.E 81g, mix, add capsule auxiliary material commonly used, make 1000 capsules by the capsule common process.
Embodiment 2
Get grape seed extract 70g, Ganodenna Lucidum P.E 110g, mix, add tablet auxiliary material commonly used, make 1000 by the tablet common process.
Embodiment 3
Get grape seed extract 120g, Ganodenna Lucidum P.E 60g, mix, add oral liquid auxiliary material commonly used, make the 1000ml oral liquid by the oral liquid common process.
Embodiment 4
Get grape seed extract 80g, Ganodenna Lucidum P.E 100g, mix, add granule auxiliary material commonly used, make 1000 bag particles by the granule common process.
Embodiment 5
Get grape seed extract 90g, Ganodenna Lucidum P.E 90g, mix, add capsule auxiliary material commonly used, make 1000 capsules by the capsule common process.
Claims (6)
1, a kind of health food with anti-oxidation function is characterized in that filling a prescription and contains the raw material of following weight portion:
Grape seed extract 70-120 part, Ganodenna Lucidum P.E 60-110 part.
2, health food according to claim 1, it is characterized in that grape seed extract directly obtains from the market purchase, its quality standard is: OPC 〉=90.0%, moisture≤5.0%, ash content≤5.0%, heavy metal≤10.0ppm, total number of bacteria≤1000cfu/g, mould and yeast≤100cfu/g.
3, health food according to claim 1, it is characterized in that Ganodenna Lucidum P.E prepares by following method: glossy ganoderma adds 8-12 times of water gaging after cutting into slices, extract 2-3 time, each 4-6 hour, merge extract, concentrate the back spray-drying; The quality requirement of Ganodenna Lucidum P.E is: polysaccharide 〉=10.0%, moisture≤8.0%, ash content≤10.0%.
4, health food according to claim 1 is characterized in that its preparation is any one formulation that pharmaceutically allows
5, health food according to claim 4 is characterized in that its formulation is tablet, granule, capsule, oral liquid, syrup or pill.
6, the preparation technology of health food as claimed in claim 1 is characterized in that comprising the following step:
Get Ganodenna Lucidum P.E and grape seed extract mixes by prescription, common process is made required preparation.
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| CN103652859A (en) * | 2013-11-13 | 2014-03-26 | 西安医学院 | Health-care food with oxidation resistance and preparation method thereof |
| CN104187629A (en) * | 2014-08-13 | 2014-12-10 | 溧阳市天目湖保健品有限公司 | Health food with function of delaying senescence and preparation method of health food |
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| CN106562407A (en) * | 2015-10-12 | 2017-04-19 | 杨芳 | Health-care food with antioxidant function |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103652859A (en) * | 2013-11-13 | 2014-03-26 | 西安医学院 | Health-care food with oxidation resistance and preparation method thereof |
| CN103652859B (en) * | 2013-11-13 | 2016-03-09 | 西安医学院 | A kind of health food with anti-oxidation function and preparation method thereof |
| CN104187629A (en) * | 2014-08-13 | 2014-12-10 | 溧阳市天目湖保健品有限公司 | Health food with function of delaying senescence and preparation method of health food |
| CN106259940A (en) * | 2015-06-26 | 2017-01-04 | 吉林肽谷生物工程有限责任公司 | Jilin Radix Ginseng oligopeptide purposes in the food preparing anti-oxidation function or health food |
| CN106562407A (en) * | 2015-10-12 | 2017-04-19 | 杨芳 | Health-care food with antioxidant function |
| CN106071018A (en) * | 2016-07-14 | 2016-11-09 | 广州六顺生物科技有限公司 | A kind of pressed candy with antitumor action and preparation method and application |
| CN108576819A (en) * | 2018-04-24 | 2018-09-28 | 北京中和鸿业医药科技有限公司 | A kind of liver-protecting combination, health food, preparation method and applications |
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