[go: up one dir, main page]

CN1778315A - Eijknisia flower honey and oral liquid - Google Patents

Eijknisia flower honey and oral liquid Download PDF

Info

Publication number
CN1778315A
CN1778315A CNA2005100715908A CN200510071590A CN1778315A CN 1778315 A CN1778315 A CN 1778315A CN A2005100715908 A CNA2005100715908 A CN A2005100715908A CN 200510071590 A CN200510071590 A CN 200510071590A CN 1778315 A CN1778315 A CN 1778315A
Authority
CN
China
Prior art keywords
nicaea
chinese mugwort
honey
eijknisia
oral liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2005100715908A
Other languages
Chinese (zh)
Inventor
叶红平
朱作霖
孙萌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HUAIBEI HUIKE PHARMACEUTICAL Co Ltd
Original Assignee
HUAIBEI HUIKE PHARMACEUTICAL Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HUAIBEI HUIKE PHARMACEUTICAL Co Ltd filed Critical HUAIBEI HUIKE PHARMACEUTICAL Co Ltd
Priority to CNA2005100715908A priority Critical patent/CN1778315A/en
Priority to PCT/CN2005/001756 priority patent/WO2006128335A1/en
Publication of CN1778315A publication Critical patent/CN1778315A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A23L21/25—Honey; Honey substitutes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/04—Drugs for disorders of the respiratory system for throat disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pulmonology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Otolaryngology (AREA)
  • Nutrition Science (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Food Science & Technology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

An Aikenisiya flower honey and its associated oral liquid as the health-care product suitable for children and old people are disclosed. It contains ponocembrin and phenolic resin. Its preparing process is also disclosed.

Description

Eijknisia flower honey and oral liquid
Invention field
But the present invention relates to a kind of brand-new Chinese mugwort Nicaea product, is exactly Eijknisia flower honey and Ai Ke Nicaea oral liquid or rather, and relevant production or preparation method.
Background of invention
But Chinese mugwort Nicaea (Echinacea, Chinese is translated into Echinacea again) original North America, the aboriginal Indian is always as medical herbs, the optimal drug of stinging, having a headache and catch a cold as treatment wound, Serpentis, generally be used to prevent and treat cold, laryngopharynx swelling and pain, influenza and wound healing, mild or moderate infection etc., can improve immunologic function, eighties of last century promptly is put into the American National pharmacopeia twenties, is one of most popular medical herbs in North America.In Europe, but be the position that the product of raw material occupies year sales volume first for several years running with the Chinese mugwort Nicaea, especially in Germany and French, but Chinese mugwort Nicaea and preparation thereof have become the standing medicine of family.Sell in the rank in U.S. medicinal plants, but the Chinese mugwort Nicaea 1995,1996,1997,1999,2002 rank the first every year, and be cited as 2002 the wind and cloud medical herbs (Herb ofthe Year, Http:// www.nres.uiuc.edu/outreach/pubs/hfs/Echinacea.pdf).
Its immunocompetence is subjected to generally attention both domestic and external, just is being widely used in medicine, supplementary and field of health care food the America and Europe.
Modern medicine study is found, but is contained multiple polysaccharide and glycoprotein, caffeic acid analog derivative and Echinacea glycosides etc. in the Chinese mugwort Nicaea.For example, polysaccharide PSI is 4-methoxyl group-glucal-arabinose-xylan polysaccharide (4-O-methy-glucuronoar-binoxylan, Mr 35000), polysaccharide PSII is acid arabinose-rhamnose-galactose polysaccharide (acidicarabinorhamnogalactan, Mr 45000), neutral fucose-galactose-xylan one glucose polysaccharide (fucogalactoxyloglucans, Mr 10000 and 25000); Acid arabinose-galactose polysaccharide (arabinogalac tan, Mr 75000) etc.But the glycoprotein that obtains from the Chinese mugwort Nicaea has three kinds, and Mr is respectively 17000,21000 and 30000.But other is isolated chemical compound and also comprises fatty acyl amide compound from the Chinese mugwort Nicaea; the chicoric acid methyl ester; 2-oxygen coffee acyl-3-oxygen-Resina Ferulae acyl group tartaric acid and 2; 3-oxygen-two Resina Ferulae acyl group tartaric acids; 2-oxo-Resina Ferulae acyl group tartaric acid and 2-oxygen-coffee acyl-3-oxygen-coumaric acyl tartaric acid; 2-oxygen-coffee acyl-3-oxygen-ferulic acid tartaric acid; 2; 3-oxygen-two-5[alpha-carbonyl-β (3; the two carbonyl phenyls of 4-) ethyl]-coffee acyl group tartaric acid and 2-oxygen-coffee acyl-3-oxygen [5-[alpha-carbonyl-β (2; the two carbonyl phenyls of 4-)-ethyl]-coffee acyl]-tartaric acid, or the like.All these materials all are proved to be has (Echinacea, The Plant That Boots Your Immune System, London, 1999, Souvenir PressLtd.) such as good raising immunity and antiinflammations.
In in the past two, 30 years, countless clinical trials has proved that from the angle of modern medicine but the Chinese mugwort Nicaea has excellent curative and drug safety, comprise born immune system defect treatment of diseases effect (Z.Phytother.1989,10,67-70. and Phytomed.1996,3,95-102).But the application of Chinese mugwort Nicaea on modern medicine mainly concentrates on it to above the immune invigoration effect, be mainly used in the treatment viral infection, the pathogenic bacteria sexuality is dyed, protozoon property disease, with (AlternativeMedicine Review such as fungal infections, 2001,6,411-414), but wherein there is six clinical trials and in vitro tests proof Chinese mugwort Nicaea juice can stimulate leukocytic generation and improve leukocytic activate the phagocytic capacity (J.Altern.Complement Med.1995,1,145-160).In surpassing 3900 people's clinical trial, proving, but Chinese mugwort Nicaea juice can reduce the sickness rate of flu and influenza well, alleviate the symptom etc. (Nutrition Science News, in JIUYUE, 1999 number and in March, 1999 number etc.) of sufferer.Find that in addition but Chinese mugwort Nicaea juice can suppress the division of virus effectively, deriving of control virus duplicate (Planta Med.1978,33,89-102).Carry out retrospective study by suffering from bronchitic child to 1280, but the result shows with the treatment of Chinese mugwort Nicaea juice than accelerating the healing process with antibiotic therapy, this is a kind of viral infection mainly due to bronchitis mostly, antibiotic is powerless to it, but but Chinese mugwort Nicaea preparation can stimulate nonspecific defense mechanism, attacks by the cell of viral infection.Find simultaneously, but the probability that the child who adopts the upper respiratory tract infection of Chinese mugwort Nicaea juice treatment falls ill again is well below blank group (U.S. pharmacy association magazine: Journal of the American Medical Association, 2003,290,2824-2830).
Though but a lot of clinical trial certificate Chinese mugwort Nicaea juice can be good at improving the immunity (Altern.Med.Rev.2002 of human body, 7,138-149), and but a lot of chemical compounds that extract from the Chinese mugwort Nicaea have been proved to be better curative effect, very complicated but but the Chinese mugwort Nicaea improves the mechanism of body immunity, up to now still can not be clear fully.But any unification compound of separating does not all have the evident in efficacy of Chinese mugwort Nicaea itself, but mainly also be to use Chinese mugwort Nicaea itself to come to patient treatment now as herb and juice, but but oral Chinese mugwort Nicaea juice preparation generally need be taken more than ten days, reach 12 weeks sometimes, though but the anxious toxicity of Chinese mugwort Nicaea juice is very low, but Chinese mugwort Nicaea juice also shows good toleration in long term toxicity test, rare side effect, but taste is not good, concerning child below 12 years old and old people, take the comparison difficulty.
And, but Chinese mugwort Nicaea juice or extract concentrated solution itself and unstable be easy to occur precipitating and influence the medical effect of product, need the adding number acid that their pH value is adjusted to subacidity and stablized, as adjust between the pH value 4.5-5.3.
Summary of the invention
(1), goal of the invention
But though the Chinese mugwort Nicaea as the history of the existing more than one thousand years of medical herbs, but and the Chinese mugwort Nicaea the long characteristics of Flos Caryophylli phase of blossoming are arranged again, may be owing to reasons such as living habit, customs, not seeing up to now has the Eijknisia flower honey appearance., promote the well-being of mankind in conjunction with the medical treatment effect and the nutrition health-care functions of Mel and Ai Ke Nicaea for better, but we have developed Chinese mugwort Nicaea new product.
But purpose of the present invention just provides brand-new Chinese mugwort Nicaea product, comprises Eijknisia flower honey and relevant oral liquid formulations, is particularly suitable for child below 12 years old and old people and takes.
(2), invention product and leading indicator detection method
Described Eijknisia flower honey, but it had both comprised all active ingredients of Chinese mugwort Nicaea, as described in the preamble background technology, contain the peculiar medical active ingredient of Mel again, such as containing pinocembrin (pinocembrin) and phenolic resins (phenolics), wherein the content of phenolic resins is between 0.5～3.0% of gross weight, and the content of pinocembrin is between 200～2000 μ g/100g anthophorids honey.
But described Chinese mugwort Nicaea oral liquid formulations, but it had both comprised all active ingredients of Chinese mugwort Nicaea, as described in the preamble background technology, contain the peculiar medical active ingredient of Mel again, such as containing pinocembrin and phenolic resins, the content of phenolic resins is between 0.5～3.0% of gross weight, and the content of pinocembrin is between 200～2000 μ g/100ml.
The Shennong's Herbal that comes out in the Han dynasty has been classified Mel in the medicine as top grade.After this all on the books in successive dynasties ancient Chinese medical classics and the herbal books to Mel.Ming Dynasty's physician's Li Shizhen (1518-1593 A.D.) is discussed in Compendium of Material Medica: " Mel, its merit of being used as medicine has five: heat clearing away also, invigorating middle warmer also, the detoxifcation also, moisturize also, pain relieving also.”
Modern scientific research and use the result show ( Http:// www.hot38.com/news/news2.htm), contain a large amount of various effective ingredient in the Mel, as polysaccharide, high-load reducing sugar, glucoseoxidase (glucose oxidase), lyase bacterium (lysozyme), various terpenoids (terpenes), pinostrobin (pinostrobin), pinocembrin (pinocembrin is translated into pinocembrin sometimes again), chrysin (chrysin), multiple phenolic resins, benzyl alcohol, or the like.Longevity not only can be improved looks and promote to Mel, also has the excellent antibiotic antiinflammatory, promotes tissue regeneration, and facilitating digestion improves immunity; improve sleep, the liver protecting, resisting fatigue promotes upgrowth and development of children; the protection cardiovascular, nourishing the lung to arrest cough promotes a series of important function such as calcium absorption.In addition, the section of U.S. research staff find Mel be again very strong antioxidant ( Http:// www.chinapropolis.com.cn/yy jk19.htm).
According to analysis medically, generally all contain pinocembrin (pinocembrin) in the Mel, its antibiotic, antiviral, gram positive bacterias such as streptococcus, staphylococcus, diphtheria corynebacterium there is stronger inhibitory action, simultaneously gastrointestinal function there is regulating action, can makes gastric acid secretion normal, facilitating digestion, can relieve oral ulcer again, and accelerating wound healing; But contain simultaneously the chemical compound with simple Chinese mugwort Nicaea juice or extract concentrated solution phenolic resins by name (phenolics) not of the same race in the Mel, phenolic resins has the antioxidative characteristic, so the oxidation resistance of Mel is near vitamin C.
Pinocembrin and phenolic resins all can adopt physicochemical data and Spectrum Analysis to identify, such as with high pressure liquid chromatography (HPLC) analytical method, can carry out qualitative and quantitative analysis.
The assay method of phenolic resins: on the spectrogram absworption peak area of various phenolic resins is being come together to calculate total content through being added in after the unified quantization;
The assay method of pinocembrin: the absworption peak on spectrogram is wanted and can significantly be observed, and peak height is minimum to be more than three times of noise peak (noise).For example adopt anti-phase C-18,5 μ m, 4.6 * 250mm (Waters) post, mobile phase is the NaH of A:30 mM 2PO 4, B: acetonitrile; 35 ℃ of temperature, the linear gradient method is: 0-28 minute: 10%B → 30%B; 28-70 minute, 30%B → 55%B; 70-75 minute, 55%B → 10%B, the retention time at pinocembrin peak (retention time) was at 48.48 minutes, and the content of pinocembrin reaches more than the 200 μ g/100g Mel.
For Eijknisia flower honey, pH value normal range is about 3.4-5.6, therefore need not add acid and come stablely, and stability is higher.
(3), the preparation method of product
The preparation method of 1 Eijknisia flower honey:
Method one obtains by bee-keeping
But the bee-keeping mode of herding that is suitable for producing Chinese mugwort Nicaea Mel big commentaries on classics ground, little commentariess on classics are arranged, three kinds of modes surely.But under the less situation of Chinese mugwort Nicaea cultivated area, preferably take to raise fixedly, be about to bee colony and raise basically, but this bee farm can be in Chinese mugwort Nicaea growing area Anywhere, but preferably be placed on the core of Chinese mugwort Nicaea growing area at fixed bee farm.
Though each kind Apis all has its different qualities, the kind that is suitable for the Apis of production Eijknisia flower honey comprises all Apiss, i.e. the Apis of Zhong Guo Apis and various countries, west is such as apis mellifera (Apismellifera ligustica; Italian bee), Ka Niela Apis (A.m.carnica; Carniolanbee), noise made in coughing or vomiting Seoul Ba Qian Apis (A.m.carpathica; Carpathian bee), caucasian (A.m.caucasica; Caucasian bee), also be included as the Apis of the various Hybrids that improve output and cultivate, import such as cultivate the gene of (Closed population breeding) method in the sealing mode some good new honeybee kinds, the new bee species of strengthening honeybee kind Asia to make honeybee kind position (antithesis) gene increase the generation of the few diploid drone of ， Yi Minus and obtaining etc.
General new Mel moisture content of gathering all is higher than 20%, and long storage time fermentation easily is rotten, therefore in addition thickening.The mode of thickening can use that cryogenic vacuum concentrates that, Red outside line concentrates, freeze drying concentrates, hot blast concentrates etc. several; Thickening temperature is generally between 4～100 ℃, between best 4～55 ℃ of the temperature commonly used; Concentrated mode preferably adopts the cryogenic vacuum method for concentration.The spissated characteristics of cryogenic vacuum are that efficient is higher, and treating capacity is also big, can guarantee that the effective therapeutic component in the Mel is not affected.The Mel sampling that obtains after concentrating is analyzed, and its water content is less than 20%.
Mel is after the thickening sterilization of gathering, and sampling analysis active constituent content wherein is to guarantee the medical curative effect of product.Effective ingredient mainly refers to pinocembrin, polysaccharide and various phenolic resins (phenolics).Said phenolic resins, such as chicoric acid (cichoric acid), chlorogenic acid (chlorogenic acid), echinacoside (echinacoside) etc., this is present international analytical standard.
But method two mixes Chinese mugwort Nicaea extract concentrated solution and general Mel
Adopt conventional method that but Chinese mugwort Nicaea extract concentrated solution and general honey are mixed, ratio generally is between 1: 1～100, and ratio commonly used is between 1: 1～10.
General Mel is meant human known all Mel, and such as following these common Mel Jujubaes, Vitex chinensis Mill. nectar, Flos Sophorae Mel, pear flower honey, sunflower honey, Semen Fagopyri Esculenti nectar, Herba Astragali Melilotoidis (Herba Astragali Sinici) nectar, Fructus Litchi nectar or the like, but the present invention comprises all Mel.
The Mel that adopts in the mixing can be the Mel before the thickening, also can be the later Mel of thickening.General new Mel moisture content of gathering all is higher than 20%, and long storage time fermentation easily is rotten, therefore in addition thickening.The mode of thickening, temperature range are like described in the method one.
Sometimes can add an amount of surfactant and defoamer in the mixing.General medicinal surfactant and defoamer commonly used all can be used for the present invention.
But the preparation method of 2 Chinese mugwort Nicaea oral liquid formulations
Method one can be made Eijknisia flower honey and the preparation of pharmaceutically acceptable carrier other dosage form and use, such as soft capsule, and oral liquid, tablet, pill, capsule, coated tablet, aerosol and other liquid preparation.
Liquid preparations such as oral liquid can be wine solution or aqueous solution, or suspension and latex etc.When selecting solid preparation for use, need to use corresponding excipient, the selection of excipient is easily for those skilled in the art in this area.
But method two also can be made oral liquid to Chinese mugwort Nicaea extract concentrated solution and the preparation of pharmaceutically acceptable carrier.Liquid preparations such as oral liquid can be wine solution or aqueous solution, or suspension and latex etc.
When selecting the oral liquid formulations mode for use, need to add suitable correctives, they can be Mel, sorbose, xylose, xylitol, glycerol, disodium glycyrrhizinate, mannitol, mannose, galactose, maltose, lactose, fructose, sucdrol, saccharin sodium, steviol glycosides, glucose and sucrose etc.
Necessary words can add suitable preservatives and antioxidant, be suitable for comprising benzoic acid (Benzoic Acid) as the material of antiseptic, sorbic acid (Sorbic Acid), ethanol (Alcohol), parabens (Parabene), benzyl alcohol (Benzyl Alcohol), and phenethanol (Phenethanolum) etc.; Be suitable for comprising sodium sulfite, sodium pyrosulfite, sodium sulfite, exsiccated sodium sulfite, sodium thiosulfate, ascorbic acid, methionine (methionine), thiourea, Calcium Disodium Versenate (EDTA-Na2), phosphoric acid, citric acid, butylated hydroxyarisol (BHA), Butylated Hydroxytoluene (BHT), nor-pair of hydrogen guaiaretic acid (CDGA), tocopherol, gallate esters etc. as the material of antioxidant.
Sometimes in preparation, also need to add an amount of aromatic, mucilage and coloring agent etc.Aromatic comprises that the spice of synthetic has essence that ester, alcohol, aldehyde, ketone, terpenoid etc. make by different proportion and fennel oil, Oleum Rosae Rugosae, rose essence, Fructus Citri Limoniae oil, Fructus Citri Limoniae essence, vanilla, vanillin, flavoring banana essence, flavoring pineapple essence, Oleum menthae, orange peel oil, apple essence etc.Mucilage thickness tool retentivity can disturb the sense of taste of taste bud and reaches the flavoring purpose, and commonly used have starch, arabic gum, tragcanth, carboxymethyl cellulose, methylcellulose, sodium alginate, pectin, an agar etc.Coloring agent is as the caramel color of synthetic and titanium dioxide etc.
Other also comprises and adds emulsifying agent and can improve liposoluble substance re-absorbed degree and increase the lipid or the fat-soluble complementary material of they of utilization rate in digestive system biology, perhaps add the diffusible complementary material of entry, perhaps two kinds of complementary materials add simultaneously, or the like.Lipid commonly used or fat-soluble complementary material comprise fertility alcohol (vitamin E, Tocopherol), vegetable oil and lecithin (Lecithin) etc.Water solublity and the diffusible complementary material of water commonly used comprise lecithin, polyoxyethylene sorbitan monoleate (Polysorbate-80) and oily lactic acid etc.Can comprise arabic gum, tragakanta, Pseudobulbus Bletillae (Rhizoma Bletillae) glue, gelatin, Lac, (sea) sodium alginate, (anhydrous) lanoline, agar, cholesterol, lecithin, Cera Flava, vaseline, spermol, stearyl alcohol, spermaceti, paraffin etc. as the material of emulsifying agent.
Sometimes to add an amount of surfactant and defoamer in the oral liquid.General medicinal surfactant and defoamer commonly used all can be used for the present invention.
(4), take mode
Eijknisia flower honey among the present invention can directly be used, and the amount of use is decided according to the situation of specific product.(Standardized) Eijknisia flower honey of general standardization (contain pinocembrin, and the weight total content of phenolic resins being no less than 0.5%), every day, sooner or later respectively once each one restrained.
But Chinese mugwort Nicaea oral liquid is decided according to production method (prescription), but as adopting Chinese mugwort Nicaea extract concentrated solution, Mel, gelatin (Gelatin), glycerol (glycerin), lecithin (Lecithin), carboxymethyl cellulose, formulated with water, (contain pinocembrin, and the weight total content of phenolic resins is no less than 0.5%) after the standardization, every bottled 5 milliliters, use oral methods, every day sooner or later each once, each one bottle; The weight total content that is mixed with phenolic resins in other words is no less than 0.5% oral liquid, every bottled 10 milliliters, every day sooner or later each once, each one bottle.
(5), beneficial effect
But Eijknisia flower honey and Ai Ke Nicaea oral liquid formulations combine the medical health care function of Chinese mugwort Nicaea juice or extract concentrated solution and Mel, have both both sides' active ingredient, contain a certain amount of pinocembrin and phenolic resins such as the product among the present invention, but make it have medical effect and function that Chinese mugwort Nicaea juice or extract concentrated solution itself can not possess, and be more suitable for child below 12 years old and old people and take, filled up the market vacancy.
Description of drawings
But Fig. 1 is a Chinese mugwort Nicaea HPLC spectrogram (UV chromatogram at 330nm);
But Fig. 2 is Chinese mugwort Nicaea characteristic fingerprint spectrogram (LCQ MS fingerprint spectrum), i.e. the characteristic fingerprint mass spectrum of Eijknisia flower honey.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.
Embodiment 1 spectrum analysis
Among Fig. 1, that the rt=5.2 branch is planted is caftaric acid, that the rt=8.8 branch is planted is chlorogenic acid (cholorgenic acid), what the rt=11.9 branch was planted is pinocembrin, that the rt=12.6 branch is planted is chicoric acid (cichoric acid), that the rt=14.5 branch is planted is hydroxyl chicoric acid (cichoric acid without oneOH group)), that the rt=14.8 branch is planted is chicoric acid methyl ester (cichoric acid methyl ester).
But employing of HPLC analytical method and the relative analysis of Chinese mugwort Nicaea extract concentrated solution master sample, the condition of HPLC analytical method commonly used is as follows: 20 minutes linear gradient methods, use anti-phase PhenomenexHydro-RP C18, (4 μ m add material granule, Phenomenex to 2.0 * 150 millimeters liquid-phase chromatographic column, Torrance, CA, USA), mobile phase speed is 0.3 ml/min, mobile phase A is 0.1% aqueous formic acid, and Mobile phase B is 0.1% formic acid acetonitrile solution.The linear gradient method is: 0-10 minute: 10%B → 30%B; 10-15 minute, 30%B → 90%B; 15-15.1 minute, 90%B → 10%B; 15.1-20 minute, 10%B.
The analyzing and testing means can be mass spectrum (MS) and UV, visible light methods such as (UV-Vis), are that a HPLC spectrogram and the UV, visible light of using the 330nm wavelength are drawn detection as Fig. 1.
But analytical method also can adopt the mode of Chinese mugwort Nicaea extract concentrated solution fingerprint characteristic spectrogram, such as electro-spray ionization (electrospray ionization, ESI) mass spectrography, but as Fig. 2 is Chinese mugwort Nicaea extract concentrated solution electro-spray ionization mass spectrum, use just ionized LCQ ion well mass spectrograph (Ion-trapMass Spectrometer), 50% acetonitrile solution is made mobile phase, and flow velocity 0.2 ml/min, mass scanning scope are that m/z=150 is between the m/z=2000.
The preparation method of embodiment 2 Eijknisia flower honey
Method one is by bee-keeping results anthophorids honey
But the center in Chinese mugwort Nicaea plantation, leeward Chaoyang, the sunshine-duration is long, and especially beehive is set up in the place that can early be arrived by solar radiation morning, adopts the wooden accurate beehive of 10 collimation marks (claiming the Lang Shi beehive again) of complying with.In two kilometers straight warp, can put 40～50 groups of Apiss.Regularly checked once every 7～10 days, check that the Entries order comprises:whether queen bee exists, queen to lay eggs and larva circle size, the sweet storage of powder deposit situation, disease and pest generation etc., and inspection is looked into Time and should be selected the sky to carry out a fine warm morning.
Need the artificial supplementation syrup to feed at the no florescence, to keep the growth of bee colony, syrup concentration is 45% sucrose based on sucrose, but the water (volume ratio) of 10% Chinese mugwort Nicaea extract concentrated solution and 45%.Mel Yi storage in nest completely can carry out gathering honey work, selects fine day to carry out.The honey of adopting is analyzed after cryogenic vacuum concentrates, and its water content is less than 20%, bottles every bottle of 50-100ml then under aseptic nitrogen protection.
The Eijknisia flower honey that obtains is carried out analyzing and testing, because every hive bee is all different, product has certain difference, so pH value is between 3.4-5.6, wherein the detection method of pinocembrin and phenolic resins as previously mentioned, the content of pinocembrin is 200-2000 μ g/100g anthophorids honey, and the content of phenolic resins is the 0.5-3.0% of gross weight.
Method two, general honey and Ai Ke Nicaea extract concentrated solution mix
Under 15～20 ℃; general honey after 10 liters of process cryogenic vacuums concentrate stirs under nitrogen protection at leisure; and add 200 milligrams medicinal methyl-silicone oil (20ppm concentration) and 50 the gram medicinal Tween-40s; after treating that Mel can stir easily; but 1.2 liters Chinese mugwort Nicaea extract concentrated solution (available from U.S. Affordable Supplements company; 4105 West Pawnee; Wichita; KS; USA) under nitrogen protection, add at leisure; mixture continues down to stir up to mix homogeneously at 20 ℃, bottles every bottle of 50-100ml then under aseptic nitrogen protection.
The Eijknisia flower honey that obtains is carried out analyzing and testing, and between the pH value 3.4-5.6, the content of pinocembrin is 200-2000 μ g/100g anthophorids honey, and the content of phenolic resins is the 0.5-3.0% of gross weight.
But the preparation method of embodiment 3 Chinese mugwort Nicaea oral liquid formulations
Method one, prepare from Eijknisia flower honey
Under 15～20 ℃; 500 milliliters sterile distilled water stirs under nitrogen protection at leisure; and add the medicinal Tween-40s that the sorbic acid of 2.1 grams, 20 milligrams medicinal methyl-silicone oil (20ppm concentration) and 5.0 restrain; to be mixed evenly after, adds 500 milliliters Eijknisia flower honey, the disodium glycyrrhizinates of 2.0 grams, the gelatin of 12 grams, the glycerol of 10 grams, the lecithin and 50 carboxymethyl celluloses that restrain of 12 grams.Under nitrogen protection, mixture continues down to stir behind mix homogeneously at 20 ℃, bottles 10 milliliters every bottle then under aseptic nitrogen protection.
But the Chinese mugwort Nicaea oral liquid formulations that obtains is carried out analyzing and testing, and the content of pinocembrin is 200-2000 μ g/100ml, and the content of phenolic resins is the 0.5-3.0% of gross weight.
But method two, make from Chinese mugwort Nicaea extract concentrated solution
Under 15～20 ℃; 1.0 the sterile distilled water that rises slowly stirring under nitrogen protection; and add 2.6 the gram sorbic acid; the medicinal Tween-40 of 20 milligrams medicinal methyl-silicone oil (20ppm concentration) and 5.0 grams; to be mixed evenly after; add 100 milliliters Mel, the disodium glycyrrhizinate of 2.0 grams, the gelatin of 12 grams, the glycerol of 10 grams, the lecithin of 12 grams and the carboxymethyl cellulose of 50 grams; after treating this mixture solution mix homogeneously, but add 200 milliliters Chinese mugwort Nicaea extract concentrated solution.Under nitrogen protection, mixture continues down to stir behind mix homogeneously at 20 ℃, bottles 10 milliliters every bottle then under aseptic nitrogen protection.
But the Chinese mugwort Nicaea oral liquid formulations that obtains is carried out analyzing and testing, and the content of pinocembrin is 200-2000 μ g/100ml, and the content of phenolic resins is the 0.5-3.0% of gross weight.

Claims (10)

1, a kind of brand-new Eijknisia flower honey, but it is characterized in that having the active ingredient of Chinese mugwort Nicaea and Mel.
2, claim 1 described Eijknisia flower honey is characterized in that containing pinocembrin and phenolic resins, and the content of pinocembrin is between 200～2000 μ g/100g anthophorids honey, and the content of phenolic resins is between 0.5～3.0% of gross weight.
3, a kind of preparation method of brand-new Eijknisia flower honey, but it is characterized in that utilizing Chinese mugwort Nicaea flower to culture Apis.
4, a kind of preparation method of brand-new Eijknisia flower honey, but it is characterized in that utilizing Chinese mugwort Nicaea extract concentrated solution and general honey to mix, but the ratio of Chinese mugwort Nicaea extract concentrated solution and general honey is between 1: 1～100.
But 5, the preparation method of claim 4 described Chinese mugwort Nicaea nectar, but the ratio of Chinese mugwort Nicaea extract concentrated solution and general honey is between 1: 1～10.
6, the application of claim 1 described Eijknisia flower honey, directly edible, or make soft capsule, oral liquid, tablet, pill, capsule, coated tablet, aerosol, or other liquid preparation.
But 7, a kind of Chinese mugwort Nicaea oral liquid formulations, but it is characterized in that having the active ingredient of Chinese mugwort Nicaea and Mel, contain pinocembrin and phenolic resins; The content of described pinocembrin is between 200～2000 μ g/100ml, and the content of described phenolic resins is between 0.5～3.0% of gross weight.
8, but claim 7 described Chinese mugwort Nicaea oral liquid formulations are wine solution or aqueous solution, or suspension and latex etc.
9, but the preparation method of Chinese mugwort Nicaea oral liquid formulations is characterized in that, and is formulated by Eijknisia flower honey and pharmaceutically acceptable carrier.
10, but the preparation method of Chinese mugwort Nicaea oral liquid formulations is characterized in that, but formulated by Chinese mugwort Nicaea extract concentrated solution and pharmaceutically acceptable carrier.
CNA2005100715908A 2005-05-28 2005-05-28 Eijknisia flower honey and oral liquid Pending CN1778315A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CNA2005100715908A CN1778315A (en) 2005-05-28 2005-05-28 Eijknisia flower honey and oral liquid
PCT/CN2005/001756 WO2006128335A1 (en) 2005-05-28 2005-10-24 Echinacea honey and its oral preparation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNA2005100715908A CN1778315A (en) 2005-05-28 2005-05-28 Eijknisia flower honey and oral liquid

Publications (1)

Publication Number Publication Date
CN1778315A true CN1778315A (en) 2006-05-31

Family

ID=36768852

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2005100715908A Pending CN1778315A (en) 2005-05-28 2005-05-28 Eijknisia flower honey and oral liquid

Country Status (2)

Country Link
CN (1) CN1778315A (en)
WO (1) WO2006128335A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1927383B (en) * 2006-09-14 2010-04-14 淮北辉克药业有限公司 Compound preparation for rapidly reducing oxidative stress and preparation method thereof

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI23657A (en) 2011-03-23 2012-09-28 Borut Gosar Honey with the addition of concentrated liquid extract from echinacea species and its production
CN110140922A (en) * 2019-05-24 2019-08-20 林艳明 A kind of ripe honey of low temperature wine

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3496625B2 (en) * 1999-05-31 2004-02-16 サンスター株式会社 Health food composition
JP2001078717A (en) * 1999-09-17 2001-03-27 Fancl Corp Food composition
RU2180586C1 (en) * 2000-11-14 2002-03-20 Башкирский государственный университет Immunostimulating and anti-inflammatory agent and method of its preparing

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1927383B (en) * 2006-09-14 2010-04-14 淮北辉克药业有限公司 Compound preparation for rapidly reducing oxidative stress and preparation method thereof

Also Published As

Publication number Publication date
WO2006128335A1 (en) 2006-12-07

Similar Documents

Publication Publication Date Title
Abad et al. Baccharis (Compositae): a review update
US7189420B2 (en) Composition comprising wenguanguo extracts, methods for preparing same and uses thereof
WO2010049542A2 (en) Plant extract hydrolysates and antibacterial product containing the same
Dai et al. Honey in traditional Chinese medicine: A guide to future applications of NADES to medicines
Bahmani et al. Pharmaceutical, phytochemical, and economical potentials of Glycyrrhiza glabra L: A review
Nikolaou et al. Accidental poisoning after ingestion of “aphrodisiac” berries: diagnosis by analytical toxicology
KR20110128385A (en) Herbal Composition and Preparation Method for Acne Improvement Using Madam Cleansing
AU2019100737A4 (en) Anti-Helicobacter Pylori Traditional Chinese Medicine composition and applications
Gbadamosi et al. Chemical constituents and in vitro antimicrobial activities of five botanicals used traditionally for the treatment of neonatal jaundice in Ibadan, Nigeria
CN105267356A (en) Morinda officinalis oligosaccharides and preparation method therefor
CN1778315A (en) Eijknisia flower honey and oral liquid
CN100382821C (en) Vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist
Tiwari Phytochemical analysis and medicinal value of Kalmegh (Andrographis paniculata Nees)
CN108324749B (en) Traditional Chinese medicine composition for treating acne and preparation method and application thereof
CN108498655B (en) Lanjin oral liquid for clearing heat and removing toxicity and preparation method thereof
CN101069704A (en) Clerodendranthus spicatus urinary-stone-resisting extract and preparing method thereof
CN1283391A (en) Extracted liquor for disinfection
CN104820029A (en) Content detection method for compound houttuynia cordata mixture
KR101758639B1 (en) A composition of lonicerae caulis, arctii fructus semen and scutellariae radix extracts for treating respiratory disease
Andrade-Cetto et al. Hypoglycemic effect of the Rhizophora mangle cortex on STZ-NA-induced diabetic rats
Chinyama Biological activities of medicinal plants traditionally used to treat septicaemia in the Eastern Cape, South Africa
RU2303993C1 (en) Treatment and prophylaxis means applicable for treating young agricultural animals and method for preventing and treating calves for gastrointestinal tract diseases
RU2311190C1 (en) Therapeutical and prophylactic drug for young agricultural animals and a method to prevent and treatment of viral respiratory diseases in calves using it
RU2303992C1 (en) Curative-prophylactic remedy for youngsters of farm animals and method for preventing and treating respiratory diseases in calves due to its application
EA021203B1 (en) Cinnamon- and basil-based composition for treating or preventing urinary infections and method for manufacture thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20060531