CN1764466A - Application of Mango and its extracts in treating diabetes and improving its symptoms - Google Patents

Abstract

The invention relates to an application of cassia sophera and an extract thereof in medicines for treating diabetes and improving symptoms of diabetes. Belongs to the field of medicinal application of traditional Chinese medicines. The invention has remarkable effect on reducing blood sugar of the diabetics; the medicine prepared by the invention can be taken by diabetics for two weeks to achieve obvious effect, and the effect is obvious in four weeks. So that the cassia sophera and the extract thereof can be used as an ideal medicine for treating diabetes and improving the symptoms thereof.

Description

Application of awns and its extracts in treating diabetes and improving its symptoms Technical field

The invention belongs to the field of medicinal application of traditional Chinese medicines.

Background technique

Cassia sophera (Cassia sophera Lipan.), a medicinal plant, belongs to the genus Cassia in the family Fabaceae. According to the "Dictionary of Traditional Chinese Medicine": "Jiangmang, also known as Jiangmang cassia, is flat in nature, non-toxic, and sweet and slippery. It has the effect of eliminating phlegm and quenching thirst, making people sleepless, and regulating the middle. It is also known as Antipyretic. The ethanol extract of the leaves of Miscanthus has the effect of relaxing the small intestine and bronchial smooth muscle of guinea pigs." In the present invention, it refers to the seed of Miscanthus.

Contents of the invention

The object of the present invention is exactly to prepare the medicine for treating diabetes and improving its symptoms by applying the radishes and its water extract.

Its water extract adopts water extraction method to be:

1. Decoction method: Use water as the solvent, heat and boil to extract the active ingredients, generally 2-4 times of extraction is required, and the amount of solvent is generally 6-20 times the amount of awns.

2. Percolation method: After cold soaking or warm soaking, a suitable temperature solvent is used to flow through the longan to dissolve the components of the longan.

3. Immersion method: cold or warm soaking of awns in a large amount of solvent. After the ingredients are dissolved and balanced, the extract is released directly.

4. Ultrasonic extraction method: After soaking the awns, put them in ultrasonic waves together with the solvent, and use the effect of ultrasonic waves to dissolve the components of awns. ,

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Correction page (Rule 91) 5. Microwave extraction method: Put the awns and the solvent together in the microwave, use the microwave to polarize the molecules, and dissolve the components.

When the present invention is used to prepare medicines for treating diabetes and improving its symptoms, it is safe to be administered orally or parenterally. In the case of oral administration, it can be administered in any conventional form, such as powder! ], granules, tablets, capsules, pills, solutions, suspensions, syrups, buccal tablets, sublingual tablets, etc.; when the drug is administered parenterally, it may take any conventional form, such as injection (such as Intravenous injection), ointment, suppository, transdermal administration, inhalation, etc.

The medicament for treating diabetes and improving its symptoms prepared by the present invention is composed of Mangosteen fruit or its water extract and solid or liquid excipients. The solid or liquid excipients used here are well known in the art. Here are a few specific examples. The powder is a powder for oral administration. Its excipients include lactose, starch, paste dextrin, calcium carbonate, synthetic or natural aluminum sulfate, magnesium oxide, magnesium stearate, sodium bicarbonate, dry Yeast, etc.; the excipients of the solution include water, glycerin, propylene glycol, simple syrup, ethanol, ethylene glycol, polyethylene glycol, sorbitol, etc.; the excipients of the ointment can be fatty oil, hydrated lanolin, vaseline , Glycerin, beeswax, wood wax, liquid paraffin, resin, high-grade wax and other combinations of hydrophobic or hydrophilic agents.

The dose of the active substance can be changed according to the way of administration, the age and weight of the patient, the severity of the disease and other similar factors. The oral dose is: 10.0-15.0mg/kg, twice a day: 8.0-10.0mg per day /kg, once a day.

The medicine of the invention has better effects of lowering blood sugar, treating diabetes and improving symptoms. Detailed ways

The beneficial effects of applying the present invention to the preparation of the drug will be further illustrated below through test examples, which include pharmacodynamic tests and clinical curative effect observation tests.

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Correction page (Rule 91) Test Example 1 The effect of the extract of awns on the Wistar rat hyperglycemia animal model 1.1 Test materials

Drugs Mango extract, provided by the New Drug Research Office of the Institute of Regenerative Medicine Science, Jilin University, batch number: 20010708; Xiaoke Pills produced by Guangzhou No. 1 Traditional Chinese Medicine Factory, approval number: (1994) No. 11146.

Animal Wistar rats, half male and half female, body weight 195g-240g, provided by the Experimental Animal Department of Jilin University, quality certificate number: Yidongzi No. 10-5110; main reagents and instruments Streptozotocin STZ produced by American Sigma Chemical Company , lot number: 89H0604

Blood glucose test strips are produced by Beijing Yicheng Bioelectronic Technology Co., Ltd.

JPS-III Rapid Blood Glucose Tester Produced by Beijing Yicheng Bioelectronic Technology Co., Ltd.

Centrifuge Beijing Medical Centrifuge Factory, model: LDZ5-2.

Fully automatic biochemical analyzer produced by Hitachi, Japan, model: Hitachi 7170A.

1.2 Test method

Test conditions and methods:

After the Wistar rats were introduced from the Animal Department of Jilin University, they were kept in the animal room of the Basic Medical College of Jilin University, with a room temperature of 22-25°C and a humidity of 30-60%. , wheat bran flour 14%, sorghum flour 10%, bone meal 2.5%, fish meal 5%, yeast 3.5%, additive 1%.

Take awn extract powder and make three doses of 5.2% (g/v), 2.6% (g/v), 1.3% (g/v) large, medium and small doses of the test liquid. Xiaoke pills were made into a test drug solution with a concentration of 20% (g/v).

110 Wistar rats, half male and half male, were introduced from the Department of Animals of Jilin University. They were acclimatized in this room for 1 week, and after fasting for 4 hours, they were weighed, blood sugar was measured, and marked. Choose blood sugar

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Correction page (Rule 91) 100 male and female rats in normal range and in good condition were used as experiments, and 10 of them were left as the normal control group. 90 Wistar rats were modeled with streptozotocin STZ at 0.054g/kg. Streptozotocin Prime STZ is made into a 2.0% solution with 0.05mol/L citrate solution pH 4.5, and it is ready-to-use. After the rats were fasted for 16 hours, the streptozotocin STZ liquid was injected intraperitoneally once according to body weight. On the 8th day, after fasting for 4 hours without food and water, the blood sugar was measured, and the model was established if the blood sugar value was greater than 10 mmol/L. Compared with the normal control group, there was a significant difference P<0.001. The 54 hyperglycemia model animals were randomly divided into five groups, each with 10-11 animals, male and female in each group. Grouped into: normal control group, model group, Xiaoke Pill group 2.0g/kg, which is equivalent to 4 times the clinical dosage; high-dose awn extract group 0.62g/kg, medium-dose group 0.31g/kg, low-dose group 0.15g/kg, equivalent to 8, 4, 2 times of the clinical dosage.

The model group was given 10ml/kg of water, and each administration group was given 10ml/kg of the test drug solution. Daily administration at around 9:00 a.m. for four consecutive weeks, blood sugar and body weight were measured every week, and the dosage was calculated once based on the new body weight. After the fourth week of administration, the animals were sacrificed, and blood was taken to measure blood routine, blood biochemical indicators, hemorheology and insulin content, and pancreas was taken for histopathological examination. The results obtained in each group are expressed as mean ( ) ± standard deviation (S), and the results and the model group are subjected to inter-group t-test to judge their significance.

1.3 Test results

1.3.1 Effects on blood sugar

In the third week after the administration, the blood sugar of the high-dose and medium-dose groups of the extracts of the longan mangosteen significantly decreased compared with the model group, P<0.05. In the fourth week, the blood sugar levels of the large and medium doses of the mango extract group and the Xiaoke Pill group were all reduced, compared with the model group, there was a significant statistical significance P<0.05, see Table 1.

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Correction Page (; Rule 91) move 1 dose

Quantity S

Table 1. The effect of the awn extract on the blood sugar level (mmol/L) of hyperglycemia model rats (1 ± s). 2. Og/kg 0.62g/kg 0.31g/kg 0.15g/kg

10 8 9 10 9 8 8.11 ± 0.53 17.52 ± 2.40 17.22 ± 3.22 17.04 ± 3.34 17.10 ± 2.6.34 ± 3.32 A week 6.19 ± 0.50 17.84 ± 3.23 15.10 ± 2.36.5.5.5.5.5.5.5.5. 0.41 15.85 ± 3.02 14.57 ± 3.02 13.59 ± 2.60 14.06 ± 2.53 15.18 ± 3.23 Three weeks 6.22: T0.31 14.05 ± 3.52 11.97 ± 2.58* 10.71 ± 2, 72* 13.26 each 5.89. 4.16 9.38±2.83* 9.07±2.78* 9.45±1.98* 11.71±3.53 Compared with the model group, *P<0.05

Test Yi ί! 2 Clinical Observations of Drug Treatment of Diabetes Patients of the Present Invention

2.1—General Information See Table 2. Table 2 Diabetic patients after drinking mango seeds

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Correction page (Rule 91) 2.2. Treatment results

2.2.1 The hypoglycemic effect of the present invention is very significant

It can be clearly seen from Table 2 that after 28 days of drinking among the 25 diabetic patients, except for 2 patients who experienced diarrhea and general discomfort, the rest had obvious hypoglycemic effects. Compared with before taking, the average blood sugar level decreased by 2.0mmol /L, in critically ill patients decreased by 14.1mmol/L. After t test analysis, t value = 3.161, when the degree of freedom N = N-1 = 23-1 = 22, p = 0.01 t = 2.819, so the hypoglycemic effect is extremely significant.

2.2.2 The longer the drinking time, the better the effect

2.2.2.1 The marked effect of drinking for one week is shown in Table 3:

Analysis of hypoglycemic effect of drinking for one week

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Correction page (Rule 91) Carry out 1: test according to Table 3, the result: t=1.962, when the degree of freedom N-l=22-l=21, t=2.080 for ρ=0·05, t=1.721 for p=0,10, so drink for a week It is effective but not significant. 2.2.2.2 The effect analysis of drinking for two weeks is shown in Table 4:

Table 4 Analysis of hypoglycemic effect of drinking for two weeks

The t test result, t=2.2637, when the degree of freedom N=N-1=22-1=21, t=2.080 for p=0.05, t=2.831 for p=0.10, so the difference is significant, that is, the effect is obvious.

2.2.2.3 Analysis of the effect of drinking for three weeks is shown in Table 5:

Correction Page (; Rule 91) Table 5 Analysis of the hypoglycemic effect of drinking for three weeks

t test results, t=2.5043, when the degree of freedom N=N-1=19-1=18, p=0.05 t=2.101 p=0.01 t=2.878, so the effect is significant.

2.2.2.4 The effect analysis of drinking for four weeks is shown in Table 6:

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Correction page (Rule 91) Patient (An) Before drinking One week after drinking d

Blood sugar level (X) Blood sugar level (χ ₂ ) (X— X ) d— 7

Al 11.0 6.7 4.3 2.566 6.584

A2 15.8 12.3 3.5 1.766 3.118

A3 11.3 10.8 0.5 -1.234 1.522

A4 9.7 8.8 0.9 -0.834 0.695

A5 12.3 8.8 3.5 1.766 3.118

A6 7.9 7.0 0.9 -0.834 0.695

A7 12.0 10.8 1.2 -0.534 0.285

A8 7.2 7.5 -0.3 -2.034 4.137

A9 9.7 7.6 2.1 0.366 0.133

A10 5.9 6.7 -0.8 -2.534 6.421

All 10.2 9.8 0.4 -1.334 1.779

A12 6.8 6.3 0.5 -1.234 1.522

A13 11.7 8.5 3.2 1.466 2.149

A14 8.9 5.9 3.0 1.266 1.602

A15 9.8 9.5 0.3 -1.434 2.056

A16 7.2 6.5 0.7 -1.034 1.069

A17 8.7 8.7 0 -1.734 3.006

A18 10.8 10.2 0.6 -1.134 1.258

A19 6.8 6.5 0.3 -1.434 1.602

A20 8.9 8.8 0.1 -1.634 2.669

A21 6.8 6.5 0.3 -1.434 1.602

A22 8.4 7.8 0.6 -1.134 1.258

A23 23.9 9.8 14.1 12.366 152.917 Sum 231.7 (N=23 ) 186.8.1(N=23) 39.9 201.251 Average 10.07 "x 8.078 Έ( 1.734

According to the t-test results, t=3.161, when the degree of freedom N=N-1=22-1=22, t=2.074 for p=0.05, t=2.819 for p=0.01, so the effect is extremely significant.

Through the analysis, it is concluded that the longer the drinking time for diabetics drinking the present invention, the more obvious the effect is, that is, drinking for one week is basically ineffective, drinking for two weeks shows the hypoglycemic effect, drinking for three weeks has a significant hypoglycemic effect, and drinking for four weeks The hypoglycemic effect is extremely significant .

2.2.3 Years of illness and analysis of drinking effect

Divide the 23 diabetic patients who drink the present invention in Table 1 into two groups: those who have been sick for more than 5 years are a group, and those who have been sick for less than 4 years are a group. Statistical analysis is carried out, and the results are -

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Correction Page (Rule 91) .1. The effect analysis of the disease for more than 5 years is shown in Table 7

Table 7 Analysis of drinking effect after illness for more than 5 years

t test results, t=1.782, when the degree of freedom N=N—1=13—1=12, t=2.197 for p=0.05, t=1.782 for p=0.01, so the effect is not significant.

2.2.3.2. The analysis of the drinking effect after the illness for less than 4 years is shown in Table 8

Table 8 Analysis of drinking effect after illness for less than 4 years

t test results, t=2.966, when the degree of freedom N=N_ 1=10—1=9, t=2.262 for p=0.05, t=3.250 for p=0.01, so the effect is significant.

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Correction page (Rule 91) 2.3 Conclusion

1. Mango seed has a significant effect on lowering blood sugar in diabetic patients.

2. Diabetic patients will be able to see the effect after taking Mangzi for two weeks, and the effect will be remarkable after four weeks.

3. If the duration of illness is shorter (less than 4 years) than if the duration of illness is longer (over 5 years), the effect of taking mango seeds is better.

The preparation of the medicament of the present invention is further illustrated below by way of examples.

Example 1

2.0g of water extract of Mango chinensis and 1000ml of water are mixed and put into 100 bottles, each 100ml contains 20mg of Mango mango extract.

Example 2

15.0g of Longmanse and 485g of medicinal starch, the two are fully mixed, packed into capsules, and made into 1000 capsules, each weighing 0.5g, containing 15mg of Longmanse.

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Correction page (Rule 91)

Claims (1)

1. Claims

   , cyperus awns preparing treatment diabetes and the application in improving the medicine of its symptom.

   , cyperus awns water extract preparing treatment diabetes and the application in improving the medicine of its symptom

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   Correct page（The 91st article of detailed rules and regulations)