CN1746660A - Method and measuring instrument for measuring crop canopy pigment ratio - Google Patents
Method and measuring instrument for measuring crop canopy pigment ratio Download PDFInfo
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Abstract
Description
技术领域technical field
本发明属植物生理仪器领域,特别涉及一种利用作物冠层反射光谱测量作物冠层色素比值的方法以及基于此方法而设计的仪器,能快速、方便地测定作物的冠层色素比值,准确地对作物生长状态情况进行评估,再根据在不同生长时期作物对水、肥的需求量实施变量施肥和灌溉,对指导作物栽培有着重要作用。The invention belongs to the field of plant physiological instruments, in particular to a method for measuring the ratio of crop canopy pigments by using crop canopy reflection spectrum and an instrument designed based on the method, which can quickly and conveniently measure the ratio of crop canopy pigments, and accurately Evaluating the growth status of crops, and then implementing variable fertilization and irrigation according to the water and fertilizer requirements of crops in different growth periods, plays an important role in guiding crop cultivation.
背景技术Background technique
关于遥感作物养分含量监测前人已经做了大量的研究,Blackmer等(1996)认为单叶的反射光谱和投射光谱受氮素缺乏的影响;Daughtry(2000)提出可以运用作物冠层的反射光谱进行冠层叶绿素含量等的评价。以往的研究,主要是将冠层光谱与同目标实验室内测定的氮素含量之间进行统计分析,筛选敏感波段和波段组合进行冠层生化组分的遥感监测。本研究经过大量试验数据分析得出可以运用结构不敏感植被指数(SIPI)来反演作物冠层的色素比值含量,色素比值我们选用类胡萝卜素和叶绿素的比值。冠层结构不敏感植被指数(SIPI)于1995年由 等提出。将SIPI定义为:Predecessors have done a lot of research on remote sensing crop nutrient content monitoring. Blackmer et al. (1996) believed that the reflection spectrum and projection spectrum of single leaves are affected by nitrogen deficiency; Daughtry (2000) proposed that the reflection spectrum of crop canopies can be used to monitor Evaluation of canopy chlorophyll content, etc. Previous studies mainly carried out statistical analysis between the canopy spectrum and the nitrogen content measured in the same target laboratory, and screened sensitive bands and band combinations for remote sensing monitoring of canopy biochemical components. After analyzing a large number of experimental data, this study concluded that the structure-insensitive vegetation index (SIPI) can be used to invert the pigment ratio content of the crop canopy. For the pigment ratio, we choose the ratio of carotenoids and chlorophyll. Canopy Structure Insensitive Vegetation Index (SIPI) was developed in 1995 by and so on. Define SIPI as:
SIPI=(R800-R445)/(R800-R680) (1)SIPI=(R800-R445)/(R800-R680) (1)
其中R800,R680,R445分别为在波长为800nm,680nm和445nm处的光谱反射率数值。大量研究表明近红外波段800nm可以用来降低叶片表面和叶片内部的部分的结构的影响。波段445nm和680nm分别是类胡萝卜素和叶绿素a的吸收峰。本文采用冠层结构不敏感植被指数(SIPI)来反演冠层类胡萝卜素和叶绿素a的比值。作物在环境胁迫等造成生理、生化物质变化进而生长状况发生变化的情况下,作物体内的类胡萝卜素和叶绿素a的相对含量发生变化,所以作物体内的类胡萝卜素和叶绿素a的相对含量可以反映作物的生长状态。Among them, R 800 , R 680 , and R 445 are the spectral reflectance values at wavelengths of 800nm, 680nm, and 445nm, respectively. A large number of studies have shown that the near-infrared band 800nm can be used to reduce the influence of the structure of the blade surface and the internal part of the blade. The bands 445nm and 680nm are the absorption peaks of carotenoids and chlorophyll a, respectively. In this paper, the canopy structure-insensitive vegetation index (SIPI) was used to retrieve the ratio of canopy carotenoids and chlorophyll a. When crops are subjected to changes in physiological and biochemical substances and growth conditions caused by environmental stress, the relative contents of carotenoids and chlorophyll a in the crops will change, so the relative contents of carotenoids and chlorophyll a in the crops can reflect The growing state of the crop.
由图1可知,开花期冠层结构不敏感植被指数与冠层类胡萝卜素和叶绿素a比值之间在不同品种不同肥水条件下存在极显著的线性相关水平,方程的决定系数为0.7207,样本数目为64个。实验的关于类胡萝卜素和叶绿素a的变化关系的研究结果如Merzlyak(1999)的研究结果相一致。均认为作物在胁迫和衰老情况下叶绿素的衰减速度较类胡萝卜素快。图1表明可以运用冠层结构不敏感植被指数(SIPI)进行作物的冠层类胡萝卜素和叶绿素a比值反演。It can be seen from Figure 1 that there is a very significant linear correlation level between the canopy structure insensitive vegetation index at the flowering stage and the ratio of canopy carotenoids and chlorophyll a under different fertilizer and water conditions of different varieties. The coefficient of determination of the equation is 0.7207, and the number of samples for 64. The experimental research results on the relationship between carotenoids and chlorophyll a are consistent with the research results of Merzlyak (1999). It is believed that the decay rate of chlorophyll is faster than that of carotenoids in crops under stress and senescence. Figure 1 shows that the canopy structure-insensitive vegetation index (SIPI) can be used to retrieve the ratio of canopy carotenoids and chlorophyll a of crops.
目前,常采用地物光谱仪进行SIPI的测量,该测量方法所需设备结构复杂、重量较大、价值昂贵、操作困难,测量数据还需要进行二次处理,所以难以推广应用。At present, ground object spectrometers are often used to measure SIPI. The equipment required for this measurement method is complex in structure, heavy in weight, expensive in value, difficult to operate, and requires secondary processing of measurement data, so it is difficult to popularize and apply.
发明内容Contents of the invention
本发明的目的是提供一种用于测量作物冠层作物冠层色素比值的新方法,根据这种方法设计的作物冠层色素比值测试仪重量轻、体积小、成本低,结构简单、使用方便、适合于大批量生产和应用。The purpose of this invention is to provide a new method for measuring the crop canopy pigment ratio of crop canopy, the crop canopy pigment ratio tester designed according to this method is light in weight, small in size, low in cost, simple in structure and easy to use , Suitable for mass production and application.
一种测量作物冠层色素比值的新方法,采用日光作为被动光源,在三个特征波长处分别对太阳入射光和被测植被的反射光进行探测,测得太阳入射光和植被反射光在蓝光、红光和近红外三个特征波段的辐射信号强度,将这六个辐射模拟信号经A/D转换,并由微控制器按冠层结构不敏感植被指数(SIPI)的计算公式进行处理,求出SIPI值,最终计算结果通过液晶显示器显示。另外,可以对测量结果进行存储和上传。本方法的关键点在于采用干涉滤光片进行滤光以产生本发明中所需的三个特征波长。A new method for measuring the ratio of crop canopy pigments, using sunlight as a passive light source, respectively detecting the incident light of the sun and the reflected light of the measured vegetation at three characteristic wavelengths. The radiation signal intensity of the three characteristic bands of , red light and near-infrared, the six radiation analog signals are converted by A/D, and processed by the microcontroller according to the calculation formula of canopy structure insensitive vegetation index (SIPI), The SIPI value is obtained, and the final calculation result is displayed on a liquid crystal display. In addition, measurement results can be stored and uploaded. The key point of this method is to use interference filters to perform light filtering to generate the three characteristic wavelengths required in the present invention.
根据上述方法而设计的作物冠层色素比值测量仪包括如附图4中六个光电探测器38、39、40、47、48、49,光学镜头53、54、55,A/D转换器42,单片机43,显示器44,六个窄带干涉滤光片35、36、37、50、51、52;六个窄带干涉滤光片分为三组,每组为光学特性相同的两个滤光片,它们的中心波长分别位于近红外(0.795-0.805μm)和红光(0.665-0.675μm)及蓝光(0.44-0.45μm)波段;测量太阳入射光信号的三个光电探测器前分别耦合近红外滤光片37、红光滤光片36和蓝光滤光片35,滤光片35、36、37前设有漫射体32、33、34,以减小日光入射角度的影响;测量植被反射光信号的三个光电探测器前设有成像物镜53、54、55,成像物镜将距离1.3m左右的植被目标成像在光的探测器的光敏面上,在成像物镜和光电探测器中间设置近红外滤光片52、红光滤光片51和蓝光滤光片50。探测器和光学部分通过镜筒连接成一个密闭整体。The crop canopy pigment ratio measuring instrument designed according to the above method includes six photodetectors 38,39,40,47,48,49 as in accompanying
仪器上的存储电路能将测量的数据存储在仪器上,用户可以通过RS232串口将数据上传到上位机PC上进行进一步分析和处理。The storage circuit on the instrument can store the measured data on the instrument, and the user can upload the data to the host computer through the RS232 serial port for further analysis and processing.
仪器上的单片机上扩展LCD显示、掉电后数据不丢失的EEPROM存储器、串行A/D转换器、信号调理电路等。Extended LCD display on the single-chip microcomputer on the instrument, EEPROM memory that does not lose data after power failure, serial A/D converter, signal conditioning circuit, etc.
仪器的操作面板跟仪器主体分离,操作面板固定在仪器手柄上,靠近操作者,方便使用。另外,手柄跟仪器主体的连接器具有3个旋转自由度。The operation panel of the instrument is separated from the main body of the instrument, and the operation panel is fixed on the handle of the instrument, which is close to the operator and is convenient to use. In addition, the connector between the handle and the instrument body has 3 rotational degrees of freedom.
该仪器的工作原理如下:利用日光(太阳光)作光源,通过6个光电传感器,在近红外和可见光三个特定波长处,分别对日光入射光和待测植被的冠层反射光进行探测,测得的六个参数,经模拟—数字转换后,由单片机进行处理得到SIPI值,所得结果由液晶显示器(LCD)显示。在本应用中,若仪器测得445nm特征波长处的入射光信号为E445、对应波长植被反射光信号为ER445;680nm特征波长处的入射光信号为E680、对应波长植被反射光信号为ER680;800nm特征波长处的入射光信号为E800、对应波长植被反射光信号为ER800,则有:The working principle of the instrument is as follows: using sunlight (sunlight) as a light source, through 6 photoelectric sensors, at three specific wavelengths of near-infrared and visible light, respectively detect the incident light of sunlight and the reflected light of the canopy of the vegetation to be measured. The six measured parameters are processed by a single-chip microcomputer to obtain the SIPI value after analog-to-digital conversion, and the obtained result is displayed by a liquid crystal display (LCD). In this application, if the instrument measures the incident light signal at the characteristic wavelength of 445nm as E 445 , the corresponding wavelength of the vegetation reflected light signal is E R445 ; the incident light signal at the 680nm characteristic wavelength is E 680 , and the corresponding wavelength of the vegetation reflected light signal is E R680 ; the incident light signal at the characteristic wavelength of 800nm is E 800 , and the reflected light signal of vegetation at the corresponding wavelength is E R800 , then:
式中k800、k680和k445为比例常数(仪器参数),由仪器的光学系统(入射部分和反射部分)、光电传感器及其适配放大器及其电路的特性参数决定。若令k680=k1k800,k445=k2k800就有In the formula, k 800 , k 680 and k 445 are proportional constants (instrument parameters), which are determined by the optical system of the instrument (incident part and reflection part), photoelectric sensor and its adaptive amplifier and the characteristic parameters of its circuit. If k 680 =k 1 k 800 , k 445 =k 2 k 800 have
公式(2)表明:只要确定仪器的待定特征常数k1,k2就可由六个光电传感器测得的信号求得SIPI值,进而得出作物冠层色素比值。Formula (2) shows that as long as the undetermined characteristic constants k 1 and k 2 of the instrument are determined, the SIPI value can be obtained from the signals measured by the six photoelectric sensors, and then the crop canopy pigment ratio can be obtained.
上述特殊光谱响应特性的光电传感器的作用是测量特定波长的光的能量。从SIPI的计算公式可以看出来,测量SIPI的关键点是求出三个特定波长的能量,三个特征波长的能量的测量是通过光电传感器实现的。光电传感器由窄带干涉滤光片、硅光电传感器及其适配放大器等组成。窄带干涉滤光片是建立在光学薄膜干涉原理上的精密光学滤光器件,其只允许中心波长附近通带内的光通过,这就产生了三个特征波长。六个窄带干涉滤光片分为三组,每组为特性相同的两滤光片,它们的中心波长分别位于近红外(0.795-0.805μm)和红光(0.665-0.675μm)波段及蓝光(0.44-0.45μm),干涉滤光片的带宽应保证在通带内光谱反射率没有明显变化,以保证SIPI的测量精度。六个硅光电传感器与六个窄带干涉滤光片组成三组光电传感器,分别用于近红外、红光和蓝光三个特征波长处入射光和植被的反射光的探测,硅光电传感器在近红外和可见光特征波长处具有较高的光谱灵敏度,其光敏面尺寸要保证在不同的日光照明条件下有足够大信号输出和线性度。三个入射光电传感器安装在仪器的上方,用来测量入射日光的强度;三个反射光电传感器安装在仪器的下方,面向被测植被,用于测量植物反射光的强度。The function of the above-mentioned photoelectric sensor with special spectral response characteristic is to measure the energy of light of a specific wavelength. It can be seen from the calculation formula of SIPI that the key point of measuring SIPI is to obtain the energy of three specific wavelengths, and the measurement of the energy of three characteristic wavelengths is realized by photoelectric sensors. The photoelectric sensor is composed of narrow-band interference filter, silicon photoelectric sensor and its adaptation amplifier. Narrowband interference filter is a precision optical filter device based on the principle of optical thin film interference, which only allows light in the passband near the central wavelength to pass through, which produces three characteristic wavelengths. The six narrow-band interference filters are divided into three groups, and each group is two filters with the same characteristics. Their central wavelengths are respectively located in the near-infrared (0.795-0.805μm) and red (0.665-0.675μm) bands and blue ( 0.44-0.45μm), the bandwidth of the interference filter should ensure that there is no significant change in the spectral reflectance within the passband, so as to ensure the measurement accuracy of SIPI. Six silicon photoelectric sensors and six narrow-band interference filters form three groups of photoelectric sensors, which are respectively used for the detection of incident light and reflected light of vegetation at three characteristic wavelengths of near infrared, red light and blue light. It has high spectral sensitivity at the characteristic wavelength of visible light, and the size of its photosensitive surface should ensure sufficient signal output and linearity under different daylight lighting conditions. Three incident photoelectric sensors are installed above the instrument to measure the intensity of incident sunlight; three reflective photoelectric sensors are installed below the instrument, facing the measured vegetation, for measuring the intensity of plant reflected light.
上述光学系统的作用是保证测量的高度和面积。光学系统包括入射日光信号传感器前面的毛玻璃、光圈和植被反射光传感器前面的接收物镜。接收物镜的焦距决定了采用的测量高度。光学系统和光电传感器通过镜简固定为一个整体。The function of the above-mentioned optical system is to ensure the height and area of measurement. The optical system includes the frosted glass in front of the incident daylight signal sensor, the aperture and the receiving objective lens in front of the vegetation reflected light sensor. The focal length of the receiving objective determines the measurement height used. The optical system and the photoelectric sensor are fixed as a whole through the mirror.
上述仪器的待定特征常数k1,k2的确定,可通过对在近红外、红光和蓝光三个特征波长处光谱反射率相等的参考板标定求得,参考板的尺寸应与仪器探测范围相符。另外,也可以用地物光谱仪求得反射率来标定。The determination of the undetermined characteristic constants k 1 and k 2 of the above instrument can be obtained by calibrating the reference plate with equal spectral reflectance at the three characteristic wavelengths of near-infrared, red light and blue light. The size of the reference plate should match the detection range of the instrument match. In addition, it can also be calibrated by obtaining the reflectance with a surface object spectrometer.
上述仪器的A/D转换器、存储器、显示器、单片机等部分的作用是将硅光电传感器适配放大电路输出的模拟信号经模拟—数字转换器转换变为数字信号,再由单片计算机按公式(3)计算,求出SIPI值由液晶显示器(LCD),最后,根据SIPI值与肥水条件线性相关系求出生长状况进行显示。测量数据可以存储在仪器的存储器中,并能通过电平转换电路将数据上传到PC。The function of the A/D converter, memory, display, single-chip microcomputer and other parts of the above-mentioned instrument is to convert the analog signal output by the silicon photoelectric sensor adaptation amplifier circuit into a digital signal through the analog-to-digital converter, and then the single-chip computer according to the formula (3) Calculate and obtain the SIPI value from the liquid crystal display (LCD), and finally, obtain the growth status according to the linear correlation between the SIPI value and the fertilizer and water conditions for display. Measurement data can be stored in the memory of the instrument, and the data can be uploaded to the PC through the level conversion circuit.
附图说明Description of drawings
为了近一步理解本发明的特点和优点,需要参照以下说明及附图。For a further understanding of the features and advantages of the present invention, reference should be made to the following description and accompanying drawings.
图1是开花期冠层结构不敏感植被指数与冠层类胡萝卜素和叶绿素a比值之间和不同品种不同肥水条件的关系;Fig. 1 is the relationship between the canopy structure insensitive vegetation index and the ratio of canopy carotenoids and chlorophyll a at the flowering stage and the different fertilizer and water conditions of different varieties;
图2是作物冠层色素比值测量仪的外形结构图;Fig. 2 is the appearance structure diagram of the crop canopy pigment ratio measuring instrument;
图3是作物冠层色素比值测量仪的原理框图;Fig. 3 is the principle block diagram of crop canopy pigment ratio measuring instrument;
图4是作物冠层色素比值测量仪内部结构示意图;Fig. 4 is a schematic diagram of the internal structure of the crop canopy pigment ratio measuring instrument;
图5为作物冠层色素比值测量仪电路图。Fig. 5 is a circuit diagram of the crop canopy pigment ratio measuring instrument.
图6为作物冠层色素比值测量仪电路板元件布局图。Fig. 6 is a layout diagram of the circuit board components of the crop canopy pigment ratio measuring instrument.
具体实施方式Detailed ways
将作物冠层色素比值测量仪垂直向下距植被冠层1.3米处,其对应地面视场范围为1m×1m,若传感器38接收的太阳光在(0.44-0.45μm)波段的光谱辐射强度为45watts/(m2),传感器39接收的太阳光在(0.665-0.675μm)波段的光谱辐射强度为90watts/(m2),传感器40接收的太阳光在(0.795-0.805μm)波段的光谱辐射强度为80watts/(m2),传感器47接收的植被冠层在(0.44-0.45μm)波段的光谱反射强度为2.25watts/(m2),传感器48接收的植被冠层在(0.665-0.675μm)波段的光谱反射强度为45watts/(m2),传感器49接收的植被冠层在(0.665-0.675μm)波段的光谱反射强度为20watts/(m2),那么由公式(2)可以计算此时植被冠层在蓝光波段的光谱反射率为5%,在红光波段的光谱反射率为50%,在红外光波段的光谱反射率为25%,由公式(1)计算植被冠层的SIPI值为-0.8。然后根据SIPI值跟色素比值的关系,求出表征作物的生长状态的参数。这个参数可以被显示、存储和上传。The crop canopy pigment ratio measuring instrument is placed vertically downward at 1.3 meters away from the vegetation canopy, and its corresponding ground field of view range is 1m × 1m. 45watts/(m 2 ), the spectral radiation intensity of sunlight received by sensor 39 in the (0.665-0.675μm) band is 90watts/(m 2 ), and the spectral radiation intensity of sunlight received by sensor 40 in the (0.795-0.805μm) band The intensity is 80watts/(m 2 ), the spectral reflection intensity of the vegetation canopy received by the sensor 47 in the (0.44-0.45μm) band is 2.25watts/(m 2 ), the vegetation canopy received by the sensor 48 is in the (0.665-0.675μm ) band spectral reflection intensity is 45watts/(m 2 ), and the spectral reflection intensity of the vegetation canopy received by sensor 49 in the (0.665-0.675μm) band is 20watts/(m 2 ), then this can be calculated by formula (2) When the spectral reflectance of the vegetation canopy is 5% in the blue light band, 50% in the red light band, and 25% in the infrared light band, the SIPI of the vegetation canopy is calculated by formula (1) The value is -0.8. Then, according to the relationship between the SIPI value and the ratio of the pigment, the parameters representing the growth state of the crop are obtained. This parameter can be displayed, stored and uploaded.
使用时应避免身体对太阳光的遮挡,并且建议测量者穿深色衣服,减少衣服反射光对冠层反射光的影响。When using it, the body should avoid blocking the sunlight, and it is recommended that the measurer wear dark clothes to reduce the influence of the reflected light of the clothes on the reflected light of the canopy.
下面我们详细说明仪器的实现方法:Below we detail the implementation of the instrument:
图1是开花期冠层结构不敏感植被指数与冠层类胡萝卜素和叶绿素a比值之间和不同品种不同肥水条件的关系。这个关系是大量光谱试验数据的总结,最终显示的结果就是由这个关系求出的。Figure 1 shows the relationship between the canopy structure-insensitive vegetation index and the ratio of canopy carotenoids and chlorophyll a at the flowering stage, and the different fertilizer and water conditions of different varieties. This relationship is a summary of a large number of spectral test data, and the final displayed results are obtained from this relationship.
图2是便携式作物冠层色素比值测量仪的外形结构图,图中1为445nm入射光传感器,2为670nm入射光传感器,3为800nm入射光传感器,这三个传感器垂直向上,测量日光在三个特征波长处的入射光强度。图中4为仪器电源开关,本仪器采用电池供电。图中5是RS232串口,可以通过串口线跟计算机的串口连接,完成测量数据的上传。图中6为445nm反射光传感器,7为670nm反射光传感器,8为800nm反射光传感器,这三个传感器垂直向下,测量选定植被的冠层在特征波长处的反射光能量。图中9为仪器跟手柄11之间的连接器,它具有3个方向的旋转自由度,用来调整仪器跟手柄的角度和高度。图中10为操作面板,操作面板固定在手柄11上,方便操作人员的操作。操作面板10上有液晶显示器13和操作按键12,液晶显示器显示测量结果,按键用来对仪器进行各种操作。图中14为被测植被,本发明中规定测量面积为1m×1m,测量高度为1.3m。Figure 2 is the appearance structure diagram of the portable crop canopy pigment ratio measuring instrument. In the figure 1 is a 445nm incident light sensor, 2 is a 670nm incident light sensor, and 3 is an 800nm incident light sensor. These three sensors are vertically upward and measure sunlight at three The incident light intensity at a characteristic wavelength. 4 in the figure is the power switch of the instrument, and the instrument is powered by batteries. 5 in the figure is the RS232 serial port, which can be connected with the serial port of the computer through the serial port cable to complete the upload of the measurement data. In the figure, 6 is a 445nm reflected light sensor, 7 is a 670nm reflected light sensor, and 8 is an 800nm reflected light sensor. These three sensors are vertically downward to measure the reflected light energy of the canopy of the selected vegetation at the characteristic wavelength. 9 in the figure is the connector between the instrument and the
图3是便携式作物冠层色素比值测量仪的原理框图,图中15为用于测量红外光特征波长处的入射光信号的传感器、16为用于测量红外光特征波长处的植被反射光信号的传感器、17为用于测量红光特征波长处的入射光信号的传感器、18为用于测量红光特征波长处的植被反射光信号的传感器、19为用于测量红光特征波长处的入射光信号的传感器、20为用于测量红光特征波长处的植被反射光信号的传感器、21~26为光电传感器的适配电路、27为带多路模拟开关的A/D(模拟—数字)转换器、28为单片计算机、29为用于显示标识作物生长状况的数值得液晶显示器、30是进行数据存储得存储器、31为数据上传而采用的串口电平转换芯片。Fig. 3 is the functional block diagram of the portable crop canopy pigment ratio measuring instrument, among the figure 15 is the sensor for measuring the incident light signal at the infrared light characteristic wavelength place, 16 is the sensor for measuring the vegetation reflected light signal at the infrared light characteristic wavelength place Sensor, 17 is a sensor for measuring the incident light signal at the characteristic wavelength of red light, 18 is a sensor for measuring the reflected light signal of vegetation at the characteristic wavelength of red light, and 19 is the sensor for measuring the incident light at the characteristic wavelength of red light signal sensor, 20 is a sensor for measuring the vegetation reflected light signal at the characteristic wavelength of red light, 21-26 is an adaptation circuit for photoelectric sensors, and 27 is an A/D (analog-digital) conversion with a multi-channel analog switch Device, 28 are single-chip computers, 29 are numerical value liquid crystal displays for displaying and marking crop growth status, 30 are memory storages for data storage, and 31 are serial port level conversion chips that are adopted for data uploading.
图4是便携式作物冠层色素比值测量仪内部结构示意图,使用时如图所示垂直向下,32、33和34为麿砂玻璃的漫射体,以减小日光入射角对信号幅度造成的影响,在此漫射体下方的35、36和37为相应波长范围(0.795-0.805μm)、(0.665-0.675μm)和(0.44-0.45μm)的窄带干涉滤光片,以滤去其它波长的太阳入射光只让特征波长处的光通过,38、39和40为三个相同的光电传感器,以测量滤光片35、36和37对应的蓝光(0.44-0.45μm)红光(0.665-0.675μm)和近红外光(0.795-0.805μm)特征波长处的太阳光照强度,53、54和55为三个相同的接收物镜,设计视场角(FOV),使仪器所要求的探测范围(如1m×1m),在仪器离植被一定距离处(如1.3m)成像在物镜焦平面的硅光电传感器47、48和49的光敏面上,50、51和52为与35、36和37同样的干涉窄带滤光片,位于接收物镜53、54、55和光电传感器47、48、49中间,以产生滤光片50、51、52相应的蓝光(0.44-0.45μm)、红光(0.665-0.675μm)和近红外光(0.795-0.805μm)特征波长处的植被反射光,这样光电传感器47、48和49分别接收植被在蓝光(0.44-0.45μm)、红光(0.665-0.675μm)和近红外光(0.795-0.805μm)光谱范围的反射光,41为1个9伏的普通干电池,它为作物冠层色素比值诊断仪提供能源,42为带多路模拟开关的A/D转换器,它将6个光电传感器的模拟信号转换为数字信号,并输出给单片机43,单片机43接收6个光电传感器获取的蓝光(0.44-0.45μm)、红光(0.665-0.675μm)和近红外光(0.795-0.805μm)特征波长范围的太阳光照强度和植被反射光强,根据公式(3)计算SIPI值,44为显示屏,它显示单片机输出的SIPI值,测量数据可以存储在数据存储器45中,需要的时候可以通过串口46将测量数据传送到上位机PC上。Fig. 4 is a schematic diagram of the internal structure of the portable crop canopy pigment ratio measuring instrument. When in use, it is vertically downward as shown in the figure, and 32, 33 and 34 are diffusers of sand glass to reduce the influence of the incident angle of sunlight on the signal amplitude. 35, 36 and 37 below this diffuser are narrow-band interference filters for the corresponding wavelength ranges (0.795-0.805μm), (0.665-0.675μm) and (0.44-0.45μm) to filter out other wavelengths The incident light of the sun only allows the light at the characteristic wavelength to pass through, and 38, 39 and 40 are three identical photoelectric sensors to measure the blue light (0.44-0.45μm) and red light (0.665- 0.675 μm) and near-infrared light (0.795-0.805 μm) at the characteristic wavelength of sunlight, 53, 54 and 55 are three identical receiving objective lenses, and the field of view (FOV) is designed so that the detection range required by the instrument ( Such as 1m * 1m), at a certain distance (such as 1.3m) from the vegetation at the instrument, it is imaged on the photosensitive surfaces of the silicon photoelectric sensors 47, 48 and 49 at the focal plane of the objective lens, and 50, 51 and 52 are the same as 35, 36 and 37 The interference narrow-band filter is located in the middle of the receiving objective lens 53, 54, 55 and the photoelectric sensor 47, 48, 49, to produce the corresponding blue light (0.44-0.45 μm) and red light (0.665-μm) of the filter 50, 51, 52 0.675 μm) and near-infrared light (0.795-0.805 μm) at the vegetation reflection light at the characteristic wavelength place, photoelectric sensors 47, 48 and 49 respectively receive vegetation in blue light (0.44-0.45 μm), red light (0.665-0.675 μm) and Reflected light in the near-infrared light (0.795-0.805 μm) spectral range, 41 is a 9-volt common dry battery, which provides energy for the crop canopy pigment ratio diagnostic instrument, and 42 is an A/D converter with a multi-channel analog switch , it converts the analog signals of the six photoelectric sensors into digital signals and outputs them to the single-chip microcomputer 43, and the single-chip microcomputer 43 receives the blue light (0.44-0.45 μm), red light (0.665-0.675 μm) and near-infrared light obtained by the six photoelectric sensors (0.795-0.805 μ m) solar illumination intensity and vegetation reflection light intensity in characteristic wavelength range, calculate SIPI value according to formula (3), 44 is display screen, it shows the SIPI value that single-chip microcomputer outputs, and measurement data can be stored in data memory 45 , when needed, the measurement data can be transmitted to the upper computer PC through the serial port 46.
图5为作物冠层色素比值测量仪电路图,56为串口电平转换电路,核心芯片是美信的MAX232。57为单片机核心电路,包括复位电路、看门狗监控电路、时钟电路等。58为模数转换电路,采用了德州仪器德12位串行精度的AD,接口简单,精度满足要求。59为光电传感器及适配电路,放大电路接成电路放大形式。60为存储器电路,采用了具有IIC总线的EEPROM,断电数据不丢失。61为液晶接口电路,本发明中采用了4位段式液晶,数据接口为串行格式。62为电源电路,本发明中采用7805将电池的9v直流电转换为5v供给系统使用。63为按键接口电路。Figure 5 is the circuit diagram of the crop canopy pigment ratio measuring instrument, 56 is the serial port level conversion circuit, and the core chip is Maxim's MAX232. 57 is the core circuit of the single-chip microcomputer, including reset circuit, watchdog monitoring circuit, clock circuit, etc. 58 is an analog-to-digital conversion circuit, which adopts AD with 12-bit serial precision from Texas Instruments. The interface is simple and the precision meets the requirements. 59 is a photoelectric sensor and an adaptation circuit, and the amplifying circuit is connected into a circuit amplifying form. 60 is a memory circuit, which has adopted an EEPROM with an IIC bus, and the data will not be lost when the power is turned off. 61 is the liquid crystal interface circuit, has adopted 4 segment liquid crystals among the present invention, and the data interface is a serial format. 62 is a power supply circuit, adopt 7805 to convert the 9v direct current of battery into 5v supply system and use among the present invention. 63 is a button interface circuit.
图6为作物冠层色素比值测量仪电路板元件布局图。图中64为操作按键;65为运算放大器;66为钳位二极管;67为滑动电阻器;68为存储器;69为微控制器;70为液晶显示器接口;71为电平转换器;72复位和看门狗芯片;73为A/D转换器;74为晶振;75为串口。Fig. 6 is a layout diagram of the circuit board components of the crop canopy pigment ratio measuring instrument. Among the figure, 64 is an operation button; 65 is an operational amplifier; 66 is a clamping diode; 67 is a sliding resistor; 68 is a memory; 69 is a microcontroller; 70 is a liquid crystal display interface; 71 is a level shifter; Watchdog chip; 73 is an A/D converter; 74 is a crystal oscillator; 75 is a serial port.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101144778B (en) * | 2007-06-19 | 2010-09-08 | 中国农业大学 | Spectral reflectance measuring device |
| CN101839979A (en) * | 2010-04-22 | 2010-09-22 | 中国农业大学 | Method and device for measuring index number of canopy vegetation of crops |
| US8222603B2 (en) | 2007-10-18 | 2012-07-17 | Siliconfile Technologies Inc. | One chip image sensor for measuring vitality of subject |
| CN108489906A (en) * | 2018-03-29 | 2018-09-04 | 河北省科学院地理科学研究所 | A kind of crop growing state multiple-angle thinking spectral detection device and its application method |
| CN113686820A (en) * | 2021-08-11 | 2021-11-23 | 江苏省农业科学院 | Crop growth information intelligent sensor |
| CN113984657A (en) * | 2021-09-26 | 2022-01-28 | 淮阴工学院 | Portable Apple Brix Meter and Apple Brix Measurement Method Based on Spectroscopic Technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101144778B (en) * | 2007-06-19 | 2010-09-08 | 中国农业大学 | Spectral reflectance measuring device |
| US8222603B2 (en) | 2007-10-18 | 2012-07-17 | Siliconfile Technologies Inc. | One chip image sensor for measuring vitality of subject |
| CN101874401B (en) * | 2007-10-18 | 2012-12-05 | (株)赛丽康 | Single-chip image sensor for measuring object vitality |
| CN101839979A (en) * | 2010-04-22 | 2010-09-22 | 中国农业大学 | Method and device for measuring index number of canopy vegetation of crops |
| CN101839979B (en) * | 2010-04-22 | 2012-07-25 | 中国农业大学 | Method and device for measuring index number of canopy vegetation of crops |
| CN108489906A (en) * | 2018-03-29 | 2018-09-04 | 河北省科学院地理科学研究所 | A kind of crop growing state multiple-angle thinking spectral detection device and its application method |
| CN113686820A (en) * | 2021-08-11 | 2021-11-23 | 江苏省农业科学院 | Crop growth information intelligent sensor |
| CN113686820B (en) * | 2021-08-11 | 2024-05-14 | 江苏省农业科学院 | Intelligent sensor for crop growth information |
| CN113984657A (en) * | 2021-09-26 | 2022-01-28 | 淮阴工学院 | Portable Apple Brix Meter and Apple Brix Measurement Method Based on Spectroscopic Technology |
| CN114166756A (en) * | 2021-12-08 | 2022-03-11 | 江西师范大学 | Device and method for measuring vegetation leaf cover |
| CN114166756B (en) * | 2021-12-08 | 2023-03-10 | 江西师范大学 | Vegetation stem leaf covering and overlapping degree measuring device and method |
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