CN1632128A - Organic selenium derived from aspergillus oryzae and its preparation method - Google Patents

Abstract

The present invention discloses an organic selenium originating from aspergillus oryzae and method for making same and belongs to the alimentary products and health products additive agent field. The organic selenium in accordance with the present invention selects aspergillus oryzae at its carrier and exists in forms of aspergillus oryzae cell or aqueous solution or dry powder. The preparation method is that the selenium compound is selected as raw materials, then a series of steps are executed, such as selenium containing culture medium preparing, aspergillus oryzae inoculating and culturing, aspergillus oryzae collecting, selenium outside cells removing by wash, sterilization or/and drying; after drying, organic selenium in aspergillus oryzae cell form, or in aqueous solution form through thalli autolysis or in dry powder form by drying the aqueous solution. The aspergillus oryzae is characterized in that the bacterial develops fast and the content of the organic selenium inside selenium-rich rice aspergillus cells is high. During the fermentation process of aspergillus oryzae, inorganic selenium can be transformed into organic selenium which is easy to metabolize and absorb for human body. Organic selenium containing aspergillus oryzae takeing the place of common aspergillus oryzae to act as food product and health products additive agents s capable of trace-quantity compensating selenium continuously and can effectively prevent and cure diseases caused by absence of selenium.

Description

Organic selenium derived from aspergillus oryzae and its preparation method

technical field

The invention belongs to the field of human food additives, and relates to organic selenium derived from aspergillus oryzae and a preparation method thereof.

Background technique

Selenium exists in various functional proteases in the body. It is a key component element of glutathione peroxidase, phospholipid hydrogen glutathione peroxidase, and 5'-deiodinase active center, and participates in the metabolism of peroxide and iodine in the body. Selenium has the ability to scavenge free radicals, Preventing cancer, anti-aging and beautifying, protecting the liver, resisting harmful heavy metals, anti-radiation, regulating immune function, etc. Selenium can obviously promote the recovery of hepatitis, cardiovascular disease, and adjuvant treatment of radiotherapy and chemotherapy for tumor patients. In 1973, the World Health Organization (WHO) believed that selenium is an essential trace element in human and animal life, and selenium supplementation can effectively prevent various diseases. The "daily dietary nutrient supply" of the Chinese Nutrition Society has listed selenium as one of the 15 daily dietary nutrients.

Studies have shown that: the physiological requirement of selenium is 40ug/d, the limit poisoning dose is 800ug/d, the recommended dietary supplement amount is 50ug/d~250ug/d, the maximum safe intake is 400ug/d, and 150ug/d for children. International organizations such as WHO have adopted this data.

The main way to prevent and treat diseases caused by selenium deficiency is artificial selenium supplementation. There are two main forms of selenium used: inorganic selenium and organic selenium. The toxicological analysis of selenium salts shows that inorganic selenium is highly toxic, and is generally only used in medicines, not in food, and its application range and measurement are limited. By biologically converting inorganic selenium into organic selenium, not only can the physiological activity and absorption rate of selenium be improved, but also its toxicity can be reduced, so it has universal edible and health care value.

Plants and microorganisms are now mainly used to convert inorganic selenium into organic selenium. The transformation methods mainly include: (1) microbial synthesis transformation method, such as selenium-enriched yeast, selenium-enriched edible fungus, etc.; (2) plant natural synthesis transformation method, such as selenium-enriched tea, selenium-enriched apple, selenium-enriched algae, etc.; (3) Plant seed germination transformation method, such as selenium-enriched malt, selenium-enriched bean sprouts, etc. The organic selenium currently used is mainly prepared by microbial synthesis and transformation. The microorganisms used include yeast and some edible fungi such as ganoderma lucidum and shiitake mushrooms. These fungi have not really penetrated into people's three meals a day, and cannot achieve the purpose of supplementing selenium.

The prior art closest to the present invention is the invention patent with publication number CN1252835A, titled "Food Added Selenium Salt from Yeast and Preparation Method of Selenium Salt from Yeast". It discloses a method for producing a selenium-enriched yeast product, comprising the steps of: preparing a mixture of yeast growth nutrients (aqueous medium); preparing a mixture in distilled water by dissolving a selenium compound in distilled water and then filtering the resulting selenium solution a selenium solution; adding the selenium solution to yeast growth nutrients and mixing to form a selenium growth mixture; adding the selenium growth mixture to a live yeast culture to form a selenium yeast growth solution and incubating with shaking; Yeast cells are recovered and concentrated from the selenium yeast growth solution; the recovered yeast cells are washed to remove extracellular selenium; and the washed yeast cells are dried.

Contents of the invention

The invention provides an organic selenium derived from Aspergillus oryzae and a preparation method thereof. Organic selenium derived from Aspergillus oryzae provides people with an organic selenium salt that is easier to absorb and utilize than inorganic selenium salts, and overcomes the shortcomings of directly supplementing inorganic selenium. In the production of food industry, Aspergillus oryzae containing organic selenium is used instead of ordinary Aspergillus oryzae, so that the products produced by it, such as soy sauce, vinegar, rice wine, etc., all contain organic selenium, and become a health food for supplementing selenium.

Aspergillus oryzae is a fungus commonly used in the food industry. It is used in the production of saccharification agents for brewing soy sauce and rice wine. It is also used in the fermentation of cellulase, amylase, pectinase, kojic acid, etc. It has a wide range of application bases and Value. Studies have found that Aspergillus oryzae has the same strong selenium-enriching ability as yeast, Ganoderma lucidum, and shiitake mushrooms.

The organic selenium derived from Aspergillus oryzae of the present invention uses Aspergillus oryzae as a carrier, and exists in the form of dried Aspergillus oryzae cells or organic selenium in the form of aqueous solution or dry powder.

The content of organic selenium in the aqueous solution of Aspergillus oryzae is 5ug/ml～200mg/ml. The content in Aspergillus oryzae dry powder is 10ug/mg～300ug/mg.

The method for preparing organic selenium derived from Aspergillus oryzae of the present invention uses selenium compounds as raw materials, and the selenium compounds include sodium selenite or/and sodium selenate. After preparing a selenium-containing medium, inoculating and culturing, collecting and washing, cell autolysis, The process of sterilizing and making finished products; it is characterized in that,

Said preparation of selenium-containing medium is to add the physiological saline solution with a concentration of 10mg/L～300g/L selenium compound into the Aspergillus oryzae fermentation medium of bean juice or/and bean sprouts juice, so that the content of selenium compound is 10mg/L L～10g/L selenium-containing medium;

Said inoculation culture is to inoculate the Aspergillus oryzae spores into the selenium-containing medium, and cultivate them for 15 to 90 hours at a temperature range of 30 to 40°C;

Said collection washing is to separate the Aspergillus oryzae cells containing organic selenium from the selenium-containing medium by centrifugation or filtration; the separated physiological saline solution or glucose aqueous solution or phosphate buffer solution isotonic with the Aspergillus oryzae cells Aspergillus oryzae cells were washed 2 to 12 times to remove extracellular selenium;

The so-called autolysis refers to the application of high-salt cell autolysis method or acid-heat cell autolysis method to lyse cells, release organic selenium, amino acids, proteins, ribose and other nutrients in the cells, and make organic selenium in the form of aqueous solution . The high-salt cell autolysis method is to make the NaCl concentration in the autolysis system reach 1% to 5%, and the temperature is between 20°C and 80°C for 5h to 40h. The acid heat cell autolysis method is to make the pH value of the autolysis system in the range of 2 to 7, the temperature range is 20°C to 70°C, and the autolysis time is 10min to 30h. After the autolysis is completed, the pH value of the autolysis system is adjusted to 6.3 with NaOH. ~7.3.

The said sterilization is to sterilize at a temperature of 90-135° C. for 15-30 minutes;

The finished product is to make the organic selenium in the form of dried Aspergillus oryzae cells; to autolyze the Aspergillus oryzae cells to produce the organic selenium in the form of aqueous solution; to dry the aqueous solution to produce the organic selenium product in the form of dry powder.

In the inoculation culture, the spores of Aspergillus oryzae can be picked and directly inoculated into the bean sprouts juice medium containing sodium selenite, cultivated at 37°C, 30-45°C and 100-200rpm for 20-40h, and the first-grade seeds can be cultivated liquid; then inoculate the primary seed liquid into the selenium-containing medium according to the inoculum amount of 10%, and cultivate it for 20-40h at 30-45°C and 100-200rpm as the secondary seed liquid; finally inoculate the secondary seed liquid according to 10% of the inoculum was inoculated into selenium-containing medium.

A more detailed method for preparing organic selenium derived from Aspergillus oryzae is divided into 7 steps:

(1) Prepare a medium that can support the growth of Aspergillus oryzae.

(2) prepare the mother liquor of selenium compound;

(3) adding the selenium compound mother liquor in the aspergillus oryzae fermentation medium to form a kind of selenium-containing medium;

(4) inoculate and cultivate Aspergillus oryzae, make Aspergillus oryzae ferment growth by culture condition;

(5) collect Aspergillus oryzae cells from Aspergillus oryzae growth medium;

(6) washing the Aspergillus oryzae cells recovered to remove extracellular selenium;

(7) Sterilize and/or dry to make dry organic selenium in the form of Aspergillus oryzae cells; autolyze the bacterial cells to make organic selenium in aqueous solution; dry the aqueous solution to make organic selenium in dry powder form.

Preparation steps of the present invention (1) Aspergillus oryzae growth medium comprises bean sprouts juice medium, bean juice medium, protein-rich beans, including soybeans, black beans, mung beans, etc., and starch-rich cereals, including barley, wheat, The liquid culture medium made of corn etc. and its by-products as the main raw material also includes the culture medium that can support the growth of Aspergillus oryzae, and it can also be a mixture of the above two or more culture mediums. Add vitamins, biotin, biological small molecules, organic and inorganic salts, minerals, elemental metals, etc. that can support the growth of Aspergillus oryzae in the medium, including but not limited to biotin, vitamin B1, vitamin B6, calcium pantothenate , inositol, copper, copper sulfate, zinc, zinc sulfate, iron, and ferric sulfate. An appropriate amount of antibiotics is also added to the culture medium to prevent bacterial infection, and the antibiotics include tetracycline, streptomycin and other antibiotics. The pH of the culture medium is controlled between 3-9, and the preferred range is controlled between 4-7.

The preparation step (2) of the present invention involves the preparation of a selenium compound mother liquor, dissolving selenium compounds such as sodium selenate, sodium selenite and other selenium salts in physiological saline, and obtaining the selenium mother liquor by filtration. Selenium compounds include sodium selenate, sodium selenite and other selenium salts or in the form of organic selenium compounds. The selenium mother liquor contains 10mg/L-300g/L of selenium compound.

The preparation step (3) of the present invention involves adding the selenium compound mother liquid into the fermentation medium of Aspergillus oryzae to form a selenium-containing medium. The final selenium compound content is 10mg/L-10g/L.

The preparation step (4) of the present invention involves the inoculation and cultivation of Aspergillus oryzae. Inoculation methods include seed liquid inoculation, direct inoculation with spores, and koji inoculation made during the production of Aspergillus oryzae, but are not limited to the above several inoculation methods. The culture temperature is 30°C to 40°C, preferably 35°C to 38°C. The cultivation time is 15h-90h, and the preferred range is 40h-70h. When liquid culture medium is used in the production process, it should be cultivated on a shaker or a shaker with a rotating speed of 50 rpm to 200 rpm, preferably in the range of 120 rpm to 170 rpm. The selected Aspergillus oryzae used in the production of selenium-enriched Aspergillus oryzae can be screened out from the environment, can be purchased directly from a strain collection center, and can also be isolated from koji or fermentation broth used in industrial production.

The preparation step (5) of the present invention involves collecting Aspergillus oryzae cells from the Aspergillus oryzae growth medium. The organic selenium-containing A. oryzae cells were separated from the selenium A. oryzae culture medium by centrifugation or filtration. The rotational speed of the centrifuge is 1000rpm-10000rpm, preferably 4000rpm-8000rpm. Filtration can use filter paper, gauze, absorbent cotton, filter funnel, suction filtration and other methods.

The preparation step (6) of the present invention involves washing the recovered Aspergillus oryzae cells to remove extracellular selenium. Wash the separated Aspergillus oryzae cells for 2 to 12 times, preferably 3 to 6 times, with the aqueous solution that is isotonic with the cells. The core problem of the washing solution is to keep consistent with the osmotic pressure of the Aspergillus oryzae cells, so as to achieve the best washing effect. The solute of the aqueous solution can be selected from inorganic salts such as NaCl, KCl, MgSO ₄ , KH ₂ PO ₄ , Na ₂ HPO ₄ , or biological small molecule solutions such as glucose, sucrose, and maltose, and polyethylene glycol, polyvinyl alcohol, starch, etc. Biomacromolecule solution such as, can also use two or more solutes to adjust the osmotic pressure of aqueous solution to be consistent with Aspergillus oryzae cell osmotic pressure. Concentrations of solutes range from 90% down-regulation to 200% up-regulation based on their isotonic concentration with the cells. Take NaCl as an example to illustrate the composition of the isotonic aqueous solution: the concentration of the isotonic NaCl aqueous solution is 0.9%, but the concentration range of NaCl can be 0.11% to 2.7%, and the aqueous solution within this range can achieve better washing effect, effectively removal of extracellular selenium.

The preparation step (7) of the present invention involves sterilizing and/or drying the Aspergillus oryzae cells to obtain the organic selenium product in the form of dried Aspergillus oryzae cells. The sterilization step may be carried out at 90°C to 135°C, preferably at 115°C to 125°C. Drying can be carried out in any drying form such as natural drying, drying, vacuum drying, freeze-drying, but not limited to the above. The obtained dried Aspergillus oryzae product contains about 10ug/mg-300ug/mg organic selenium.

Organic selenium in the form of aqueous solution: the cells of Aspergillus oryzae that have been washed away with extracellular selenium are autolyzed, the cells are lysed, and nutrients such as organic selenium, amino acids, proteins, and ribose in the cells are released to make organic selenium in the form of aqueous solution. Bacterial cell autolysis adopts high-salt cell autolysis method, or acid heat cell autolysis method. The bacterial cells are autolyzed and then filtered to remove insoluble substances such as cell walls, and the obtained substances are sterilized at a temperature between 90°C and 135°C, preferably between 115°C and 125°C. The sterilization time is 10 minutes to 2 hours, preferably 20 minutes to 25 minutes. The final aqueous organic selenium product contains 5ug/ml-200mg/ml organic selenium. NaCl is selected as the salt in the high-salt cell autolysis method, and NaCl in solid form or high-concentration NaCl aqueous solution is added to make the NaCl concentration in the autolysis system reach 1% to 5%, preferably 2.5% to 3.5%. Constant temperature autolysis, the autolysis temperature can be between 20°C-80°C, the preferred range is between 50°C-60°C, and the autolysis time can be 5h-40h, preferably 20h-30h.

The acid-heat cell autolysis method is to add HCl to the Aspergillus oryzae cells that have washed away extracellular selenium, so that the pH value of the autolysis system is in the range of 2 to 7, the preferred pH value is 3.5 to 4.5, and the temperature range is 20 ° C to 70°C, the preferred temperature is 40°C-50°C, the autolysis time is 10min-30h, and the autolysis time is preferably 30min-2h. After the completion of autolysis, adjust the pH value of the autolysis system to 6.3-7.3 with NaOH.

Organic selenium in the form of dry powder: the aqueous solution obtained after cell autolysis is made into organic selenium in the form of dry powder by natural drying, drying, vacuum drying, freeze-drying, etc. It is carried out at 135°C, preferably at 115°C to 125°C, and the sterilization time is 10min to 2h, preferably 20min to 25min. The dry powder contains 10ug/mg～300ug/mg organic selenium.

The organic selenium of the present invention uses Aspergillus oryzae as a carrier, and the Aspergillus oryzae has strong selenium enrichment ability, and has the characteristics of fast growth of strains and high content of organic selenium in the selenium-enriched Aspergillus oryzae cells. In the method of the present invention, Aspergillus oryzae can convert inorganic selenium into organic selenium during the fermentation process. This organic selenium exists in the body cells of Aspergillus oryzae and is easily metabolized and absorbed by the human body. It is the optimal selenium for human use. source of selenium. Using Aspergillus oryzae containing organic selenium instead of common Aspergillus oryzae in the production of food industry can provide a simple and continuous method for supplementing trace element selenium for people with selenium deficiency; as an additive for food and health products, it can achieve trace and continuous Selenium supplementation can effectively prevent and treat various diseases caused by selenium deficiency.

Detailed ways

The concrete implementation steps of the present invention are as follows, but do not imply the limitation to the scope of the present invention:

Example 1

First prepare the liquid bean sprouts juice culture medium. Then prepare the sodium selenite mother liquor of 10g/L, absorb a certain amount of mother liquor and join in the bean sprouts juice culture medium, make the final concentration of sodium selenite in the culture medium be 150mg/L. Pick Aspergillus oryzae spores and directly inoculate them into the bean sprouts juice medium containing sodium selenite, and cultivate them at 37°C and 150rpm for 24 hours as the primary seed liquid; Sodium bean sprouts juice culture medium, 37 ℃, 150rpm culture 24h, as secondary seed liquid; The secondary seed liquid is inoculated in the growth medium according to the inoculum amount of 10%, and the growth medium also contains sodium selenite bean sprouts juice The culture medium was cultured at 37° C. and 150 rpm for 60 hours, and centrifuged at 8000 rpm for 30 minutes to collect Aspergillus oryzae cells. Wash with normal saline for 3 times to remove extracellular selenium compounds, and then sterilize with damp heat at 121°C for 20min. drying the sterilized aspergillus oryzae cells at 60°C to prepare the organic selenium in the form of dried aspergillus oryzae cells.

In Example 1, the culture temperature of the primary seed liquid and the secondary seed liquid can be cultivated at 30-45° C. and 100-200 rpm for 20-40 hours. The cultivation effect is the same in the above-mentioned ranges of temperature, rotation speed and time.

Example 2

First prepare the liquid bean juice culture medium. Prepare the sodium selenite mother liquor of 5g/L again, draw a certain amount of mother liquor and join in the bean sprouts juice culture medium, make the final concentration of sodium selenite in the culture medium be 100mg/L. Pick Aspergillus oryzae spores and directly inoculate them into the bean sprouts juice medium containing sodium selenite, cultivate them for 20 hours at 37°C and 130 rpm, and use them as the primary seed liquid; inoculate the primary seed liquid into the growth medium according to the inoculum amount of 10%. Among them, the growth medium also contains sodium selenite bean sprouts juice medium, 30 ℃, 130rpm, culture 90h, 8 layers of gauze filter to collect Aspergillus oryzae cells. Wash 12 times with aqueous glucose solution to remove extracellular selenium compounds, add solid NaCl until the concentration reaches 3%, autolyze for 24 hours at 55°C, filter with filter paper to remove insoluble matter such as cell walls, and sterilize with damp heat at 121°C for 25 minutes. Prepare organic selenium in aqueous solution.

Example 3

First prepare the liquid bean juice culture medium. Prepare the sodium selenite mother liquor of 50mg/L again, draw a certain amount of mother liquor and join in the bean sprouts juice culture medium, make the final concentration of sodium selenite in the culture medium be 10mg/L. Pick Aspergillus oryzae spores and directly inoculate them into the bean sprouts juice medium containing sodium selenite, cultivate them at 37°C and 100 rpm for 20 hours, and use them as the primary seed liquid; inoculate the primary seed liquid into the growth medium according to the inoculum amount of 10%. Among them, the growth medium also contains sodium selenite bean sprouts juice medium, 37 ° C, 100 rpm, cultivated for 48 hours, and collected Aspergillus oryzae cells by filtering through 8 layers of gauze. Wash twice with aqueous glucose solution to remove extracellular selenium compounds, add solid NaCl until the concentration reaches 2.5%, autolyze at 50°C for 30 hours, filter with filter paper to remove insoluble matter such as cell walls, and sterilize with damp heat at 121°C for 25 minutes. Prepare organic selenium in aqueous solution.

Example 4

First prepare the liquid bean juice culture medium. Prepare the sodium selenite mother liquor of 100g/L again, draw a certain amount of mother liquor and join in the bean sprouts juice culture medium, make the final concentration of sodium selenite in the culture medium be 5g/L. Pick Aspergillus oryzae spores and directly inoculate them into the bean sprouts juice medium containing sodium selenite, cultivate them at 37°C and 100 rpm for 20 hours, and use them as the primary seed liquid; inoculate the primary seed liquid into the growth medium according to the inoculum amount of 10%. In this method, the growth medium also contains sodium selenite bean sprouts juice medium, cultured at 40° C. and 300 rpm for 15 hours, and collected Aspergillus oryzae cells by filtering through 8 layers of gauze. Wash 10 times with aqueous glucose solution to remove extracellular selenium compounds, add solid NaCl until the concentration reaches 4%, autolyze at 60°C for 20 hours, filter with filter paper to remove insoluble matter such as cell walls, and sterilize with damp heat at 115°C for 30 minutes. Prepare organic selenium in aqueous solution.

Example 5

First prepare the liquid bean sprouts juice culture medium. Prepare the sodium selenite mother liquor of 15g/L again, draw a certain amount of mother liquor and join in the bean sprouts juice culture medium, make the final concentration of sodium selenite in the culture medium be 100mg/L. Pick some spores from the preserved Aspergillus oryzae strains and inoculate them into the bean sprouts juice medium containing sodium selenite, culture at 37° C. and 150 rpm for 70 hours, and collect Aspergillus oryzae cells by suction filtration. Wash 3 times with phosphate buffer to remove extracellular selenium compounds, add HCl to pH 4.5, 50°C, autolyze for 1.5h, 4000rpm, remove insoluble matter, and freeze-dry the supernatant to make dry powder, 121 ℃, sterilize for 20 minutes. Obtain organic selenium in dry powder form.

Example 6

First prepare the liquid bean sprouts juice culture medium. Prepare the sodium selenite mother liquor of 100mg/L again, draw a certain amount of mother liquor and join in the bean sprouts juice culture medium, make the final concentration of sodium selenite in the culture medium be 20mg/L. Pick some spores from the preserved Aspergillus oryzae strains and inoculate them into bean sprout juice medium containing sodium selenite, culture at 37° C. and 150 rpm for 24 hours, and collect Aspergillus oryzae cells by suction filtration. Wash twice with phosphate buffer to remove extracellular selenium compounds, add HCl to pH 3.5, 40°C, autolyze for 1h, 4000rpm, remove insoluble matter, freeze-dry the supernatant to dry powder, 125°C , sterilized for 20min. Obtain organic selenium in dry powder form.

Claims (4)

1. An organic selenium derived from Aspergillus oryzae, characterized in that it uses Aspergillus oryzae as a carrier and exists in the form of dried Aspergillus oryzae cells or organic selenium in the form of aqueous solution or dry powder. 2. According to the organic selenium derived from Aspergillus oryzae according to claim 1, it is characterized in that the organic selenium of 5 ug/ml～200 mg/ml is contained in the Aspergillus oryzae aqueous solution; or 10 ug/mg～300 ug is contained in the Aspergillus oryzae dry powder /mg of organic selenium. 3. A method for preparing organic selenium derived from Aspergillus oryzae according to claim 1, using selenium compounds as raw materials, and the selenium compounds include sodium selenite or/and sodium selenate, after preparing selenium-containing medium, inoculating and culturing, collecting The process of washing, autolyzing cells, sterilizing, and making finished products; it is characterized in that, Said preparation of selenium-containing medium is to add the physiological saline solution with a concentration of 10mg/L～300g/L selenium compound into the Aspergillus oryzae fermentation medium of bean juice or/and bean sprouts juice, so that the content of selenium compound is 10mg/L L～10g/L selenium-containing medium; Said inoculation culture is to inoculate the Aspergillus oryzae spores into the selenium-containing medium, and cultivate them for 15 to 90 hours at a temperature range of 30 to 45°C; Said collection washing is to separate the Aspergillus oryzae cells containing organic selenium from the selenium-containing medium by centrifugation or filtration; the separated physiological saline solution or glucose aqueous solution or phosphate buffer solution isotonic with the Aspergillus oryzae cells Aspergillus oryzae cells were washed 2 to 12 times to remove extracellular selenium; The so-called cell autolysis refers to the application of high-salt cell autolysis method or acid heat cell autolysis method to lyse cells and release nutrients such as organic selenium, amino acids, proteins, ribose, etc. in the cells to make an organic solution in the form of an aqueous solution. selenium. The high-salt cell autolysis method is to make the NaCl concentration in the autolysis system reach 1% to 5%, and autolyze at 20°C to 80°C for 5h to 40h. The acid heat cell autolysis method is to keep the pH value of the autolysis system at 2-7, 20°C-70°C, autolysis for 10min-30h, and adjust the pH value of the autolysis system to 6.3-7.3 with NaOH after the autolysis is completed. The said sterilization is to sterilize at a temperature of 90-135° C. for 15-30 minutes; The finished product is to make the organic selenium in the form of dried Aspergillus oryzae cells; or autolyze the Aspergillus oryzae cells to make the organic selenium in the form of aqueous solution; or dry the aqueous solution to make the organic selenium product in the form of dry powder. 4. According to the preparation method of organic selenium derived from Aspergillus oryzae according to claim 3, it is characterized in that said inoculation culture is to pick Aspergillus oryzae spores and directly inoculate them into the bean sprout juice medium containing sodium selenite , 37 ° C, 20 ~ 40 h at 30 ~ 45 ° C and 100 ~ 200 rpm to cultivate a first-level seed liquid; then inoculate the first-level seed liquid into the selenium-containing medium according to the inoculation amount of 10%, at 30 ~ 45 Cultivate at 100-200 rpm for 20-40 hours as the secondary seed liquid; finally inoculate the secondary seed liquid into the selenium-containing medium according to the inoculum amount of 10%.