CN1628523A - A kind of synergistic bactericidal composition and effervescent agent thereof - Google Patents

Abstract

The invention relates to a synergistic bactericidal composition and an effervescent agent thereof, which contain bacillus subtilis, jinggangmycin, propiconazole or thifluzamide with synergistic effective dose. The composition has obvious synergistic effect on the prevention and treatment of rice sheath blight, and the effervescent agent is convenient to use and has excellent prevention and treatment effect.

Description

A kind of synergistic bactericidal composition and effervescent agent thereof

(1) Technical field

The present invention relates to a new synergistic bactericidal composition for preventing and treating rice sheath blight and an effervescent agent thereof, more specifically, the present invention relates to a synergistically effective amount of Bacillus subtilis and Jinggangmycin, Propiconazole or Thiofuramide.

(2) Background technology

Rice is one of the main food crops for human beings. Rice is planted all over China, Japan, Thailand and other countries, covering an area of hundreds of millions of mu. Sheath blight (Rhizoctonia solani kühn) is the most common and major disease in rice planting, which occurs almost every year, and the higher the planting level, the more serious the disease is. Therefore, in the main rice planting areas, every season of rice round stalks and budding, no matter whether the disease occurs or not, the pesticides for preventing and controlling rice sheath blight must be applied once or twice.

At present, the main pesticides used for the control of rice sheath blight are polyoxin, Jinggangmycin (Daowensan), isomycin, propiconazole, thifuramide and Bacillus subtilis. However, the control effects of these pesticide products on rice sheath blight are still unsatisfactory.

In addition, the existing dosage forms of these products are mostly water-soluble powder, wettable powder, water, water-soluble granule and water suspension. When these preparations are used, they need to be mixed with water and sprayed, and farmers must carry heavy sprayers and medicinal liquids to trek and spray in the muddy water of paddy fields.

Therefore, there is still a need for a more ideal rice sheath blight control agent and a formulation that is convenient to use. The present inventors found that combining Bacillus subtilis with Jinggangmycin, propiconazole or thifuramide has a synergistic bactericidal effect, and these bactericidal compositions can be formulated into effervescent agents, thus completing the present invention.

(3) Contents of the invention

【Purpose of the invention】

The object of the present invention is to provide a synergistic bactericidal composition and its effervescent agent, which can be used for preventing and treating rice sheath blight.

【Technical solutions】

Specifically, the present invention provides a fungicidal composition, which contains a synergistically effective amount of Bacillus subtilis and jinggangmycin, propiconazole or thifluxamide as active ingredients.

Preferably, in the fungicidal composition of the present invention, the weight ratio of the active ingredient Bacillus subtilis to Jinggangmycin, propiconazole or thifluxamide is 1-250:250-1. More preferably, the weight ratio of Bacillus subtilis and Jinggangmycin is 1-180: 180-1, the weight ratio of Bacillus subtilis and propiconazole is 1-200: 200-1, and the weight ratio of Bacillus subtilis and thifur The weight ratio of amides is 1-250:250-1.

In the present invention, Bacillus subtilis can be its various strains, preferably such as BS-208 strains, Y3 strains, Y2-10 strains, 9-5-5 strains and "Libao" and "Baikang" commercial preparations. strain. When they are mixed with Jinggangmycin, propiconazole, thifuramide, etc. to control rice sheath blight, they all show obvious synergistic effect.

The BS-208 strain, the Y3 strain, the Y2-10 strain, and the 9-5-5 strain of Bacillus subtilis are all known strains. See, for example "Bacillus subtilis BS-208 fungicide achievement identification material compilation" (Wuhan Tianhui Biological Engineering Co., Ltd. August 2003)

Jinggangmycin, propiconazole or thiafluxamide are all known pesticides.

The fungicidal composition of the present invention can be formulated into various dosage forms. In the present invention, the fungicidal composition of the present invention is preferably formulated as an effervescent agent.

Effervescent A dosage form containing a basic compound (such as carbonates, bicarbonates, etc.) in combination with a solid water-soluble acidic compound (such as an organic acid). In the water medium, the alkaline substance reacts with the solid water-soluble acidic substance to generate carbon dioxide gas, which disintegrates the effervescent agent and releases the active ingredients in the preparation to achieve the effect of preventing and controlling diseases and insect pests. Effervescent agents can be produced, for example, in the form of granules or tablets.

Therefore, the present invention also provides a kind of effervescent agent of above-mentioned bactericidal composition, and its composition and content are as follows:

Active ingredient 0.5-50%, preferably 10-40%;

Disintegrant Acidic compound 4%-25%, preferably 5-20%;

  Basic compounds 4%-25%, preferably 5-20%;

Binder 2%-30%, preferably 5-20%;

Surfactant 1%-20%, preferably 3-15%;

Disintegration aid 1%-20%, preferably 3-15%;

Filler Balance

The effervescent agent of the present invention may also contain 1-15% of high penetration agent.

The adjuvants that can be used in the effervescent agent of the present invention are required not to chemically react with the active ingredients of the pesticide used to prevent and control rice sheath blight, nor to kill the bacterial spores in the preparation. Preferably, good permeability is required.

All kinds of auxiliary agents that can be used in the present invention are enumerated as follows:

The acidic compound is selected from one or more of the following substances: citric acid, tartaric acid, malic acid, adipic acid, boric acid, potassium dihydrogen citrate, potassium hydrogen tartrate, sodium fumarate and lactic acid;

The basic compound is selected from one or more of the following substances: sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate and calcium carbonate;

The binder is selected from one or more of the following substances: polyethylene glycol, polyvinylpyrrolidone and polyvinyl acetate copolymer, and PEG (12,000-20,000) in isopropanol or ethanol;

The surfactant is selected from one or more of the following substances: sodium dodecylbenzenesulfonate, tea dry powder, sodium lignosulfonate, calcium lignosulfonate;

The disintegrating agent is selected from one or more than two of the following substances: starch, sodium carboxymethylcellulose, carboxypropylcellulose and polyvinylpyrrolidone;

The filler is selected from one or two or more of the following substances: light calcium carbonate, diatomaceous earth, kaolin, sericite powder and white carbon black.

The hyperosmotic agent is selected from one or two of the following substances: azone, thiazone and NNO.

Other adjuvants, such as anti-caking agents, may also be included to increase the pesticide effect or processability.

Rhizoctonia solani, the causative agent of rice sheath blight, overwinters in the form of sclerotium. In spring, the sclerotium grows hyphae and infects rice plants from the base leaf sheaths.

Before the effervescent agent of the present invention is used, the paddy field is properly irrigated with a little more water to keep the water depth at 1cm-2cm. After the effervescent agent particles or tablets are thrown into the water, acid-base neutralization reaction foaming and tumbling will occur in the water, which is used as a driving force to make the effervescent agent The active ingredient in the effervescent granules or tablets is dispersed in the water throughout the paddy field. In addition, with the help of the penetration of the high-osmotic agent, the antibacterial active substances in the water infiltrate and infiltrate upward along the rice stem, reaching a position above 1cm from the water surface, and reaching the base of the rice leaf sheath, thereby preventing the infection of pathogenic mycelium. After spraying, reduce the irrigation depth in the paddy field to about 1cm. In order to make the preparation last longer, the spores of some strains of Bacillus subtilis which have obvious inhibitory effect on rice sheath blight can be prepared into raw powder, and mixed into its combination according to the ratio obtained by screening. In the process, they are made into granules or pressed into tablets, and thrown into the paddy fields. These spores can germinate into vegetative bodies and proliferate, and the water film at the base of the seedlings formed by the osmotic agent moves to colonize and proliferate at a position 1-2 cm higher than the water surface, so as to prolong the control effect.

【Beneficial effect】

The composition of the invention has obvious synergistic effect on the control of rice sheath blight, and the effervescent agent is convenient to use and has excellent control effect.

The present invention will be further described below with non-limiting examples.

(4) Specific implementation methods

Example 1

Bacillus subtilis BS-208 mixed with Jinggangmycin effervescent granules (or effervescent tablets)

a. Formula

Bacillus subtilis BS-208 bacteria powder (1000cfu/g) 20%

Jinggangmycin (200,000 units/g) 12%

Sodium bicarbonate 4.3%

Sodium Carbonate 5.8%

Citric Acid 8.5%

Fumaric Acid 1.8%

Polyethylene Glycol 15%

Sodium dodecylbenzenesulfonate 6.0%

Sodium Carboxymethyl Cellulose 6.0%

Azone (40% powder) 6.0%

Diatomaceous earth Balance

b. Manufacturing process

Calculate the feeding amount of each material according to the above ratio, and weigh and take out each material accurately. Start the mixing machine, first mix BS-208, Jinggangmycin, diatomaceous earth, sodium carboxymethylcellulose, citric acid, and fumaric acid, then add azone and mix well, and finally add basic compounds and other auxiliaries and mix well. After being pulverized by a micro-powder machine, the fineness of the material can reach more than 200 mesh. Pack into a granulator (extrusion granulation method) for one-time molding extrusion granulation (or tabletting), the particle diameter of the granules is about 2 mm, and each tablet weighs 1-3 grams. The dried granules are packaged as finished products after passing the inspection.

Example 2

Bacillus subtilis preparation "Baikang" mixed with Jinggangmycin effervescent granules (or effervescent tablets agent)

a. Formula

Bacillus subtilis preparation "Baicang" (commercially available) 20%

Jinggangmycin (200,000 units/g) 14%

Sodium bicarbonate 5.0%

Sodium Carbonate 5.5%

Citric acid 9.0%

Fumaric Acid 1.2%

Polyethylene Glycol 20%

Sodium dodecylbenzenesulfonate 6.0%

Sodium Carboxymethyl Cellulose 5.0%

Azone (40% powder) 7.0%

Diatomaceous earth balance

b. Manufacturing process

Calculate the feeding amount of each material according to the above ratio, and weigh each material accurately. Start the mixing machine to mix diatomaceous earth, "Baikang", Jinggangmycin, and carmellose evenly, then add the binder polyethylene glycol, azone, fumaric acid, and citric acid and mix evenly, and finally add Alkaline compounds and other various additives are mixed again. After being pulverized by a micro-powder machine, the fineness of the material can reach more than 200 mesh. Use an extrusion granulator to form granules or tablets at one time. After the dried granules (tablets) pass the inspection, they can be packaged and leave the factory.

Example 3

Bacillus YZ-10 bacteria powder mixed with Jinggangmycin effervescent granules (or effervescent tablets)

a. Formula

Bacillus subtilis YZ-10 powder (5×10 ¹⁰ cfu/g) 20%

Jinggangmycin (200,000 units/g) 12%

Sodium Carbonate 9.2%

Citric acid 9.0%

Polyethylene glycol 18%

Sodium dodecylbenzenesulfonate 4.0%

Tea dry powder (200 mesh) 8.0%

Starch 8.0%

Thione (40% powder) 5.0%

Diatomaceous earth Balance

b. Manufacturing process

Calculate and accurately weigh various materials according to the above formula. Start the mixing machine to fully mix the Bacillus bacterium powder, Jinggangmycin, tea dry powder, and thioketone. Then pre-mix the adhesive, diatomaceous earth, and starch, and in the third step, add the acidic substance and pre-mixed additives into the mixer and fully mix them with Jinggangmycin. Finally, add the remaining additives and mix well. Use a micronizer to pulverize the material to more than 200 mesh. Granulate with extruding granulator or tabletting machine tabletting, particle size or sheet weight are the same as embodiment 1. The dried granules (or tablets) can be packaged and leave the factory after passing the inspection.

Example 4

Bacillus subtilis BS-208 mixed with full ears effervescent granules (or effervescent tablets)

The trade name of Thifluzamide is "Mansui". If it is prepared with the original drug, dissolve it with acetone or ethyl acetate, mix it with diatomaceous earth quantitatively, and use it after drying; if it is prepared with 23% water suspension, directly absorb it with diatomaceous earth, mix well and dry it use.

a. Formula

BS-208 bacterial powder (1000cfu/g) 15%

"Man Sui" (fold pure) 1.8%

Sodium bicarbonate 8.5%

Sodium Carbonate 8.5%

Citric acid 9.0%

Tartaric acid 5.2%

Polyethylene Glycol 15%

Calcium lignosulfonate 6.0%

Sodium Carboxymethyl Cellulose 8.0%

Azone (40% powder) 5.0%

Diatomaceous earth Balance

b. Manufacturing method

Accurately weigh various materials according to the ratio of various materials in the formula. Add various materials to the mixer in the order of (full ears + diatomaceous earth + carboxymethyl cellulose) + BS-208 bacteria powder + calcium lignosulfonate + thione + citric acid + tartaric acid, and mix while adding. After fully mixing, add the remaining materials in the order of (Na ₂ CO ₃ +NaHCO ₃ +polyethylene glycol), then fully mix and pulverize, and then granulate with an extrusion granulator or tablet with a tablet machine. Particle diameter (or sheet weight) is the same as in Example 1. After quick drying, the product quality inspection is carried out, and the products that pass the inspection are packaged and leave the factory.

Example 5

Bacillus subtilis BS-208 mixed with propiconazole effervescent granules (or effervescent tablets)

The trade names of propiconazole are "Tuo Li Te", "Tilt" and so on. Quantitatively weigh some of the original drug of propiconazole, add appropriate amount of methanol to dissolve, and absorb with light calcium carbonate to make the content above 30% (W/W).

a. Formulation:

BS-208 bacterial powder (1000cfu/g) 20%

Propiconazole 5.0%

Sodium bicarbonate 6.5%

Sodium Carbonate 6.5%

Citric acid 6.0%

Tartaric acid 5.5%

Polyethylene Glycol 20%

Sodium dodecylbenzenesulfonate 3.0%

Tea dry powder (200 mesh) 6.7%

Sodium Carboxymethyl Cellulose 5.0%

Azone (40% powder) 6.0%

Light calcium carbonate balance

b. Manufacturing method:

Calculate the amount of various materials according to the ratio of the above formula, weigh out the various materials accurately, and mix propiconazole, light calcium carbonate, sodium carboxymethyl cellulose, and BS-208 bacterial powder in the mixing machine Evenly, then add citric acid, tartaric acid, sodium dodecylbenzenesulfonate and tea dry powder, and mix well. Add NaHCO ₃ , Na ₂ CO ₃ , mix while adding, finally add polyethylene glycol, and mix thoroughly. Granulated by an extrusion granulator or compressed by a tablet press, with a particle size of 1-2 mm (tablet weight 1-3 grams), and air-dried. After passing the inspection, it is packaged and shipped from the factory.

Example 6

"Baikang" and "Mansui" mixed effervescent granules

The pretreatment method of " full ear " is the same as embodiment 7

a. Formulation:

"Baicang" bacteria powder (commercially available) 15%

"Man Sui" (fold pure) 2.0%

Sodium bicarbonate 5.5%

Sodium Carbonate 8.0%

Citric acid 7.0%

Tartaric acid 6.0%

Polyethylene glycol 17%

Sodium lignosulfonate 8.0%

Sodium Carboxymethyl Cellulose 8.0%

Azone (40% powder) 6.0%

Diatomaceous earth balance

Manufacturing method:

Calculate the dosage of each material according to the proportion of the above formula, and weigh out each material accurately. In the mixer, firstly mix "Baikang", "Mansui", diatomaceous earth and sodium carboxymethyl cellulose, then add sodium lignosulfonate and thione, and mix well. Then add citric acid and tartaric acid and mix well. Finally NaHCO ₃ , Na ₂ CO ₃ , and polyethylene glycol were added, mixing as they were added. After mixing thoroughly, pulverize and granulate or compress into tablets with a tablet machine. Particle size sheet is the same as in Example 7. After passing the inspection, the products are packaged and leave the factory.

Implementation column 7

"Baikang" mixed with propiconazole effervescent granules (or effervescent tablets)

The pretreatment method of propiconazole is with embodiment 5.

a. Formulation:

"Baicang" bacteria powder (commercially available) 15%

Propiconazole 5.0%

Sodium bicarbonate 5.0%

Sodium Carbonate 8.0%

Citric acid 6.5%

Tartaric acid 6.0%

Fumaric acid 0.8%

Polyethylene Glycol 20%

Sodium dodecylbenzenesulfonate 3.0%

Tea dry powder (200 mesh) 8.0%

Sodium carboxymethylcellulose 6.2%

Azone (40% powder) 5%

Light calcium carbonate balance

b. Manufacturing method:

Calculate the amount of various materials according to the ratio of the above formula, weigh out the various materials accurately, and mix propiconazole, light calcium carbonate, sodium carboxymethyl cellulose, and BS-208 bacterial powder in the mixing machine Evenly, then add citric acid, tartaric acid, fumaric acid, sodium dodecylbenzenesulfonate and tea dry powder, and mix well. Add Na ₂ CO ₃ , NaHCO ₃ , mix while adding, finally add polyethylene glycol, and mix well. After being pulverized, use an extrusion granulator to granulate or compress into tablets with a tablet press, with a particle size of 1-2 mm (or tablet weight of 1-3 grams), and air-dry. After passing the inspection, it is packaged and shipped from the factory.

It has been confirmed by indoor virulence test that Bacillus subtilis BS-208 strain, Y3 strain, Y2-10 strain, 9-5-5 strain, "Libao" and "Baikang" commercial preparations, etc. Combination of cycloconazole and thifluzamide has obvious synergistic effect on the control of rice sheath blight. Various combinations and their synergistic effects are listed in Table 1.

Table 1: The ratio and synergistic effect of the combination of Bacillus subtilis and Jinggangsu Component A (Bacillus subtilis) Component B Weight ratio A:B(W/W) Synergistic effect size (CTC value) BS-208 Jinggangmycin propiconazolamide (1～180):(180～1)(1～200):(200～1)(1～200):(200～1) 130～1100230～1800180～1350 Y3 Jinggangmycin propiconazolamide (1～150):(180～1)(1～180):(180～1)(1～250):(250～1) 147～816210～1080165～1430 Y2-10 Jinggangmycin propiconazolamide (1～150):(180～1)(1～150):(180～1)(1～200):(220～1) 132～779166～917157～880 9-5-5 Jinggangmycin propiconazolamide (1～120):(120～1)(1～100):(150～1)(1～150):(180～1) 123～617130～546168～912 Resilience Jinggangmycin propiconazolamide (1～150):(150～1)(1～100):(150～1)(1～200):(200～1) 130～1100131～497135～660 Bacam Jinggangmycin propiconazolamide (1～150):(150～1)(1～100):(150～1)(1～250):(250～1) 120～1250126～590134-650

Claims (10)

1. A bactericidal composition, which contains a synergistically effective amount of Bacillus subtilis and jinggangmycin, propiconazole or thifuroxan as active ingredients. 2. The fungicidal composition according to claim 1, wherein the weight ratio of Bacillus subtilis to Jinggangmycin, propiconazole or thifuroxan is 1-250:250-1. 3. according to the bactericidal composition of claim 2, wherein, the weight ratio of Bacillus subtilis and Jinggangmycin is 1-180: 180-1, the weight ratio of Bacillus subtilis and propiconazole is 1-200: 200- 1. The weight ratio of Bacillus subtilis to Thiofuramide is 1-250:250-1. 4. The fungicidal composition according to any one of claims 1-3, wherein the Bacillus subtilis is Bacillus subtilis BS-208, Y3, Y2-10, 9-5-5, Lipo and Bacam. 5. according to the effervescent agent of the arbitrary bactericidal composition of claim 1-4, its composition and content are as follows: Active ingredient 0.5-50% Disintegrant Acidic compound 4%-25%                                                                                                                                                     4% - 25 % Adhesive 2%-30% Surfactant 1%-20% Disintegration aid 1%-20% Filler Balance 6. The effervescent agent according to claim 5, which also contains 1-15% of hyperosmotic agent. 7. The effervescent agent according to claim 5 or 6, wherein, The acidic compound is selected from one or more of the following substances: citric acid, tartaric acid, malic acid, adipic acid, boric acid, potassium dihydrogen citrate, potassium hydrogen tartrate, sodium fumarate and lactic acid; The basic compound is selected from one or more of the following substances: sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate and calcium carbonate; The binder is selected from one or more of the following substances: polyethylene glycol, polyvinylpyrrolidone and polyvinyl acetate copolymer, and PEG (12000-20000) in isopropanol or ethanol; The surfactant is selected from one or more of the following substances: sodium dodecylbenzenesulfonate, tea dry powder, sodium lignosulfonate, calcium lignosulfonate; The disintegrating agent is selected from one or more than two of the following substances: starch, sodium carboxymethylcellulose, carboxypropylcellulose and polyvinylpyrrolidone; The filler is selected from one or two or more of the following substances: light calcium carbonate, diatomaceous earth, kaolin, sericite powder and white carbon black. 8. The effervescent formulation according to claim 6 or 7, wherein the hyperosmotic agent is selected from the group consisting of azone, thiazone and NNO. 9. The application of the fungicidal composition according to any one of claims 1-4 to control rice sheath blight. 10. The application of the effervescent agent according to any one of claims 5-8 to control rice sheath blight.