CN1687766A - Fibrous electrophoresis chip and preparation method - Google Patents
Fibrous electrophoresis chip and preparation method Download PDFInfo
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- CN1687766A CN1687766A CN 200510025271 CN200510025271A CN1687766A CN 1687766 A CN1687766 A CN 1687766A CN 200510025271 CN200510025271 CN 200510025271 CN 200510025271 A CN200510025271 A CN 200510025271A CN 1687766 A CN1687766 A CN 1687766A
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Abstract
本发明属环境监测技术领域，具体为一种纤维电泳芯片及其制备方法。该纤维电泳芯片由纤维束代替原电泳芯片中的玻璃毛细管而构成。其中纤维束由单丝的玻璃纤维无捻粗纱或化学纤维长丝无捻粗纱组成。其制备方法是将纤维束经脱脂、清洗和干燥后浸入浸渍液如甘油，得甘油浸渍纤维束。将纤维束按进样和分离毛细管的结构形成置于有机玻璃片或膜上，利用甲基丙烯酸甲酯注模原位聚合和光引发冷法聚合方法，将纤维束包埋，最后在纤维束一端注水加压，将甘油洗出。该电泳芯片利用纤维束中的孔隙作为电泳的进样和分离通道。该纤维电泳芯片制作简便、价格低廉，可批量生产，在环境监测、临床诊断和食品分析等领域中有良好的应用前景。
The invention belongs to the technical field of environmental monitoring, in particular to a fiber electrophoresis chip and a preparation method thereof. The fiber electrophoresis chip is composed of fiber bundles instead of the glass capillary in the original electrophoresis chip. The fiber bundle is composed of monofilament glass fiber roving or chemical fiber filament roving. The preparation method is to immerse the fiber bundles in an impregnating liquid such as glycerin after degreasing, washing and drying to obtain the glycerin-impregnated fiber bundles. The fiber bundle is placed on a plexiglass sheet or membrane according to the structure of the sampling and separation capillary, and the fiber bundle is embedded by injection molding in-situ polymerization and photo-induced cold polymerization method of methyl methacrylate, and finally at one end of the fiber bundle Inject water and pressurize to wash out the glycerin. The electrophoresis chip uses the pores in the fiber bundle as the injection and separation channels for electrophoresis. The fiber electrophoresis chip is easy to manufacture, low in price, can be produced in batches, and has good application prospects in the fields of environmental monitoring, clinical diagnosis, food analysis and the like.
Description
Technical field
The invention belongs to the environmental monitoring technology field, be specifically related to a kind of fibrous electrophoresis chip and preparation method thereof.
Background technology
Since [1] such as nineteen ninety A.Manz has proposed micro-full analytical system (since the μ-TAS) first, as a frontier interdisciplinary, its target is to handle whole microminiaturized, the integrated and portability that detects by micro electronmechanical processing (MEMS) technology and biotechnology realization chemical analysis system from sample, is the important directions and the forward position of present analytical instrument development.Capillary electrophoresis chip in the micro-fluidic chip is the microanalysis technology that analytical chemistry circle acquisition is at home and abroad in recent years extensively paid attention to, i.e. capillary slot on etching on the matrixes such as silicon, glass, plastics, with cover closure good after, utilize the electric field separates material, it is achieved the whole process of capillary electrophoresis separation material on more than one square centimeters substrate.The capillary electrophoresis chip electrophoresis is an architectural feature with the microchannel network, it is the emphasis of current micro-total analysis system development, and efficient with it, fast, few, the low consumption of reagent dosage and integrated level advantages of higher caused domestic and international analysis and life science circle relevant expert's extensive concern, shown good prospects for application in fields such as environmental monitoring, clinical diagnosis, Pharmaceutical Analysis, legal medical expert and military affairs, various new electrophoresis chip preparations and detection technique emerge in an endless stream.
Electrophoretic microchip mainly uses glass and polymer chip [2], and the glass-chip process technology requires high, needs specialized apparatus, is difficult to adopt mould to be produced in enormous quantities, and the price comparison costliness has limited its application.So polymer chip is developed, wherein polymethylmethacrylate (PMMA) and dimethyl silicone polymer (PDMS) are two kinds of polymkeric substance commonly used [3].Technology such as injection moulding, die and casting are mainly adopted in the making of polymer chip, but the capillary slot on the plastic chip of making has metaboly, with design load certain difference is arranged, and the reappearance of chip chamber of the same race is not good.Usually chip electrophoresis all is to carry out in the kapillary in chip.After the inventor finds fibrous bundle flooded special impregnant such as glycerine first, can be embedded in the organic glass sheet by the organic hyaline monomer in-situ polymerization, end boring at fibrous bundle is exposed in the hole it, after impregnant passes through the aperture flush away, space in the fibrous bundle can replace traditional kapillary to carry out electrophoretic separation, thereby has proposed this new technology of fibrous electrophoresis chip and new ideas.
List of references
[1]Manz?A，Graber?N，Widmer?HM.Sens.Actuators?B?1990，1，244-248.
[2]Verpoorte?E.Electrophoesis?2002，23，677-712.
[3]Becker?H，Locascio，LE.Talanta?2002，56，267-287.
Summary of the invention
The objective of the invention is to propose a kind of easy to make, with low cost, detect fast, the fibrous electrophoresis chip of favorable reproducibility and preparation method thereof, it utilizes organic hyaline monomer methyl methacrylate injection molding in-situ polymerization and light-initiated cold process polymerization technique, uses fibrous bundle and organic glass sheet to be made into fibrous electrophoresis chip.Fibrous electrophoresis chip system proposes first.
The fibrous electrophoresis chip that the present invention proposes, its structure is the same with the structure of sample introduction electric current chip capillaceous as separating with the common kapillary of making of organic glass, as shown in Figure 1, it is placed on the chip 3 by separation capillary 2 and 7 square crossings of sample introduction kapillary and constitutes.Place, two ends capillaceous is respectively solution connection holes 1,6,4,5, and organic glass cover plate 8 is arranged above.Among the present invention, sample introduction kapillary 7 and separation capillary 2 adopt fibrous bundle, and the diameter of fibrous bundle is the 100-300 micron.It is the monofilament of 10-30 micron that fibrous bundle can adopt 50-200 root, every diameter, and woven glass roving fabric or chemical fiber filament (as terylene etc.) roving is formed.
The fibrous electrophoresis chip that the present invention proposes be to utilize space in the fibrous bundle as electrophoretic separation and sample intake passage, thereby the kapillary that replaces original organic glass to make is realized electrophoresis detection.
Owing to adopt fibrous bundle as electrophoretic separation and sample intake passage, therefore, the making of electrophoresis die chip is with original different.The making step of electrophoresis core of the present invention is as follows:
The woven glass roving fabric of the monofilament that fibrous bundle is used or chemical fiber filament roving immerse maceration extract (as glycerine) then through degreasing, cleaning and dried, get limpid glycerine impregnation of fibers bundle.To need the glycerine impregnation of fibers bundle of length (as 3-10 centimetre) to place on an organic glass sheet or the organic glass film, wherein, a short fibrous bundle becomes cross vertical with the long fibre bundle, the point of crossing is the tie point of two fibrous bundles, and fibrous bundle is fixed on organic glass sheet or the organic glass film because of the viscosity of glycerine.Thermal initiator and light trigger are dissolved in the organic hyaline monomer methyl methacrylate, and heating makes the monomer solution pre-polymerization become glycerine shape clear solution.With a middle hollow out is that the big or small silicone rubber plate of chip size (rectangle) is placed on the organic glass sheet or organic glass film of fibrous bundle, makes fibrous bundle in middle hollow out rectangle.Above-mentioned pre-gathering solutions is filled with the cavity of hollow out shape, again another sheet organic glass cover plate is covered on cavity, compress and extrude bubble, use UV-irradiation, cause bulk polymerization, fibrous electrophoresis chip base sheet.One side at the base sheet is bored solution connection holes in the end points place of two bundle fiber bundles, and the ends exposed that makes fibrous bundle is in solution connection holes.A solution connection holes place pressure water injection, glycerine is washed out, get the fibrous electrophoresis chip finished product.
The present invention further describes as follows to above-mentioned fibrous electrophoresis chip method for making:
With thickness is the size (as 7 centimetres of 2 cm x) that the organic glass sheet of 1-2 millimeter is cut into to be needed, remove surface protection film after, standby after drying up after clean with washing agent and washing.To contain woven glass roving fabric that some (as the 50-200 root) nominal diameters are 10-30 micron monofilament or chemical fiber filament (as terylene) roving through chloroform or alcohol degreasing, washing agent cleans and drying after, immerse water miscible thickness maceration extract (as glycerine), propose then, strain the two ends of fiber and remove the glycerine droplet that oozes at fiber surface, get limpid glycerine impregnation of fibers bundle with filter paper.To need the glycerine impregnation of fibers bundle of length (as 3-10 centimetre) to place on the organic glass sheet of a cleaning or the organic glass film 12 that thickness is the 100-120 micron, the glycerine impregnation of fibers bundle that to lack (as 1 centimetre) places from long fibre Shu Yiduan a distance (as 0.4-0.6 centimetre) also vertical with it, the right-angled intersection point is the tie point of two fibrous bundles, long fibrous bundle 2 is used for electrophoretic separation, and short fibrous bundle 7 is used for sample introduction.Fibrous bundle 2,7 viscosity because of glycerine are fixed on organic glass sheet or the organic glass film 12.Because the viscosity of glycerine is big, the bonding bunchy of roving can be prevented to scatter.The representative diameter of fibrous bundle is the 100-300 micron.
With organic hyaline monomer methyl methacrylate and small amount of thermal initiating agent azoisobutyronitrile (consumption is the 0.1-0.2% of monomer mass) and a little light initiating agent styrax (consumption is the 0.1-0.2% of monomer mass), in 50 ℃ of water-baths heating and shake and make its dissolving, in 80-90 ℃ of water-bath, heated 10-15 minute then, make the monomer solution pre-polymerization become the clear solution of glycerine shape.Because the present invention need use pre-gathering solutions embedding glycerine impregnation of fibers bundle, so the pre-polymerization time can not be oversize, otherwise pre-gathering solutions viscosity is excessive, can make glycerine impregnation of fibers movement and deformation during embedding.Generally in pre-collecting process, to prevent that sealing enters, avoid temperature too high simultaneously, otherwise can be sudden and violent poly-because of causing, the waste of material caused.There is silicon rubber (the being dimethyl silicone polymer) sheet 9 (thickness is 0.5-1mm) of chip size (rectangle) size to be placed on the organic glass plate or film 12 of fibrous bundle a middle hollow out, make fibrous bundle in middle hollow out rectangle, constitute mould cavity 10, above-mentioned pre-gathering solutions is filled with mould cavity, the organic glass sheet 8 that is the 1-2 millimeter with another sheet thickness covers on mould cavity 10 again, compresses and extrude bubble.The uviol lamp that with wavelength is 365 nanometers causes bulk polymerizations by organic glass sheet 8 irradiation pre-gathering solutions, get fibrous electrophoresis chip base sheet (Fig. 3 (b)), the distance of chip and uviol lamp is 4-6 centimetre, when temperature is the 20-25 ℃ of left and right sides, needs about 25-35 minute polymerizable complete.Also can be in polymerization under the sunshine, when temperature is the 20-25 ℃ of left and right sides, need about 50-70 minute polymerizable complete.One side at the base sheet is bored solution connection holes 1,4,5 and 6, aperture such as 1-3 millimeter, and the boring point is the end of two bundle fiber bundles, and the degree of depth in hole requires to make the ends exposed of fibrous bundle in solution connection holes.The fibrous bundle chip that produces that prevents to hole enters during boring.By pressurizeing with syringe a solution connection holes, 60-70 ℃ hot water is pressed in the fibrous bundle, glycerine is washed out, the base sheet after the boring gets fibrous electrophoresis chip finished product (Fig. 3 (c)) through deburring.Electrolytic solution is injected fibrous bundle by the solution hole on the chip, can carry out electrophoretic separation.
Fibrous electrophoresis chip of the present invention is made poly (methyl methacrylate) plate, fibrous bundle glass fiber and the man-made fiber large-scale production of using, and is with low cost.The fibrous electrophoresis chip electrophoresis path that what deserves to be explained is is difficult for stopping up, and has solved the easily stifled problem of common electrophoresis chip capillary at all.The fibrous electrophoresis chip that the present invention makes is easy and simple to handle, favorable reproducibility, highly sensitive, the range of linearity is wide, amount of samples is few, can be used for fields such as environmental monitoring, clinical diagnosis, life science, food analysis and industrial on-line analysis.
Description of drawings
Fig. 1 is the structural diagrams of the typical fibers electrophoresis chip that the present invention relates to.
Fig. 2 is the structural drawing (exploded view) of fibrous electrophoresis chip in-situ polymerization device among the present invention.
Fig. 3 makes process flow diagram for fibrous electrophoresis chip among the present invention.(a) prepare the fibrous electrophoresis chip synoptic diagram for light-initiated situ aggregation method; (b) the sandwich sandwich fibrous electrophoresis chip base sheet outward appearance for the present invention relates to; (c) be in fibrous bundle is terminal after boring solution connection holes finished fiber electrophoresis chip outward appearance.
Fig. 4 is for using Amperometric Detection Coupled fibrous electrophoresis chip separating phenol (a), the 2-chlorophenol (b) and 2 of the technology of the present invention preparation, the electrophoresis pattern of 3-chlorophenesic acid (c).
Fig. 5 leads the electrophoresis pattern that the detection fibers electrophoresis chip separates potassium ion (a), sodion (b) and lithium ion (c) for the electricity that uses the technology of the present invention preparation.
Number in the figure: 1,6,4,5 is the solution hole, 2 is separation capillary (also being the long fibre bundle), 7 is sample introduction kapillary (also being the staple fibre bundle), and 3 is substrate, and 8 is the organic glass glass cover-plate, 9 is silicone rubber plate, 10 is mould cavity, and 11 is a side opening of silicone rubber plate 9, and 12 is organic glass sheet or organic glass film, 13 is glass plate, and 14 is fibrous electrophoresis chip base sheet.
Embodiment
Further describe the present invention below by embodiment and accompanying drawing:
1, the making of Amperometric Detection Coupled fibrous electrophoresis chip
(A) location of fibrous bundle on poly (methyl methacrylate) plate
With thickness is the small pieces that 1 millimeter commodity poly (methyl methacrylate) plate is cut into 2 centimetres wide and 7.5 centimeter length, remove surface protection film after, it is standby to dry up the back with washing agent and washing after clean.With alkali-free glass fibre roving (containing 120 nominal diameters approximately is 14 microns monofilament) after the chloroform degreasing, clean the back air dry with absolute ethyl alcohol and water respectively, propose after immersing glycerine, strain the two ends of fiber and remove the glycerine droplet that oozes at fiber surface, get limpid glycerine impregnation of fibers bundle with filter paper.With length is that 6.5 centimetres glycerine impregnation of fibers bundle places on the organic glass sheet 12 of a cleaning, the glycerine impregnation of fibers bundle of another 1 centimeter length is placed from long fibre Shu Yiduan 0.5 centimeters and vertical with it, the right-angled intersection point is the tie point of two fibrous bundles, long fibrous bundle 2 is used for electrophoretic separation, and staple fibre bundle 7 is used for sample introduction.Fibrous bundle 2,7 viscosity because of glycerine are fixed on the organic glass sheet 12.Because the viscosity of glycerine is big, the bonding bunchy of alkali-free glass fibre roving can be prevented to scatter.The diameter of fibrous bundle is about 200 microns.
(B) embedding and the fibrous electrophoresis chip of fibrous bundle in chip made
With organic hyaline monomer methyl methacrylate and small amount of thermal initiating agent azoisobutyronitrile (be about monomer mass 0.15%) and a little light initiating agent styrax (be about monomer mass 0.2%), in 50 ℃ of water-baths heating and shake and make its dissolving, in 85 ℃ of water-baths, heated about 12 minutes then, shook mixed solution once in per 3 minutes, and made molten this pre-polymerization of monomer become the clear solution of glycerine shape viscosity shape.Because the present invention need use pre-gathering solutions embedding glycerine impregnation of fibers bundle, so the pre-polymerization time can not be oversize, otherwise pre-gathering solutions viscosity is excessive, can make glycerine impregnation of fibers movement and deformation during embedding.Pre-polymerization later stage polymerization speed is accelerated, and should stop heating when bubble occurring immediately, and cools off rapidly with cold water.In pre-collecting process, to prevent that sealing enters, avoid temperature too high simultaneously, otherwise can be sudden and violent poly-because of causing, the waste of material caused.Be that hollow outs are the rectangle of chip size rectangle size in the middle of silicon rubber (dimethyl silicone polymer) sheet 9 of 0.8mm with thickness, be placed on then on the organic glass sheet 12 of fibrous bundle, make fibrous bundle in middle hollow out rectangle, constitute mould cavity 10, above-mentioned pre-gathering solutions is filled with mould cavity, the organic glass cover plate 8 that with another sheet thickness is 1 millimeter again covers on mould cavity 10, carefully compress and extrude bubble, be that the uviol lamp of 365 nanometers causes bulk polymerizations by organic glass cover plate 8 irradiation pre-gathering solutions with wavelength then, get fibrous electrophoresis chip base sheet 14 (Fig. 3 (b)), the distance of chip and uviol lamp is 5 centimetres, when temperature is 20 ℃ of left and right sides, need about 30 minutes polymerizables complete.One side at the base sheet is bored solution connection holes 1,4,5 and 6,2 millimeters in aperture, and the boring point is the end of all fibres bundle, and the degree of depth in hole reaches the ends exposed of fibrous bundle in solution connection holes.The chip that produces that prevents from during boring to hole enters fibrous bundle.It is intrafascicular with syringe pressurization 70 ℃ hot water to be pressed into all fibres a solution connection holes, and glycerine is washed out, and the base sheet after the boring gets fibrous electrophoresis chip finished product (Fig. 3 (c)) through deburring.Electrolytic solution is injected fibrous bundle by the solution hole on the chip, can carry out electrophoretic separation.To after the cutting of the chip end (right side) among Fig. 1 the end of defibre bundle be exposed, get the Amperometric Detection Coupled fibrous electrophoresis chip, can be used for the styletable Amperometric Detection Coupled.
(C) application of Amperometric Detection Coupled fibrous electrophoresis chip
The Amperometric Detection Coupled fibrous electrophoresis chip that the present invention makes successfully is used for the compartment analysis of phenols environmental contaminants, concrete visible following test experiments result:
Utilization structure glass fibre electrophoresis chip and 0-3000V high-voltage DC power supply and ampere detector formation glass fibre electrophoresis chip Amperometric Detection Coupled system as shown in Figure 1, the 100 μ M phenol, the 2-chlorophenol and 2 that obtain, the electrophoresis pattern of the electrophoretogram of 3-chlorophenesic acid is seen Fig. 4.Test condition is: separation voltage is+2000V, sample introduction voltage is+2000V, and sample injection time is 3s, and buffer solution is 5mM borax-5mM phosphate buffer (pH8.5), detecting electrode is the carbon disk electrode of diameter 200 μ m, and the detection current potential is 0.95V (with respect to the Ag/AgCl electrode).The range of linearity is 0.1 μ mol/L-1000 μ mol/L, is limited to 0.08-0.1 μ mol/L under detecting.Measure 100 μ M phenol, 2-chlorophenol and 2 10 times, the relative standard deviation of 3-chlorophenesic acid peak-to-peak signal is respectively 3.1%, 2.5% and 4.5%, show that this noumenal modification polymethyl methacrylate micro flow control chip range of linearity is wide, reappearance is good, fast efficient, in 200 seconds, just can separate and detect simultaneously three kinds of phenolic comp ' ds pollution fully, can be used for the mensuration of actual sample.
2, electricity is led the making of detection fibers electrophoresis chip
(A) location of fibrous bundle on poly (methyl methacrylate) plate
Because micro-fluidic chip non-contact conductance detecting electrode need be as far as possible near the passage in the chip, to improve detection sensitivity, the need used thickness is that the organic glass film about 100 microns replaces the organic glass sheet among the embodiment 1 to come load glycerine impregnation of fibers bundle.Electricity is led the detection fibers electrophoresis chip and is mainly used in isolating ions, so fibrous bundle need use neutral material, present embodiment is selected the polyester filament yarn for use, get terylene roving (contain 200 nominal diameters approximately and be about 6 microns monofilament) after the untwisting, clean the back air dry with absolute ethyl alcohol and water respectively, the length of the dipping of polyester filament fibrous bundle, the size of chip, fibrous bundle is equal to embodiment 1.The diameter of fibrous bundle is about 250 microns.With thickness is the small pieces that 1 millimeter commodity poly (methyl methacrylate) plate is cut into 2 centimetres wide and 7.5 centimeter length, remove surface protection film after, it is standby to dry up the back with washing agent and washing after clean.Be coated in the pre-gathering solutions of embodiment 1 on a slice glass sheet in addition and be pressed on another glass sheet, distance between two glass plates can be controlled by the transparent dacron membrane that sticks 100 microns of thickness in the inboard, four limits of glass plate, with wavelength is that the irradiation of 365 nano-ultraviolet lights causes bulk polymerization, and after the demoulding the organic glass film, and be cut into small pieces with organic glass sheet same size.Organic glass film and mould glass bonding very firm, the demoulding can be heated workpiece 1 minute in 85 ℃ of water-baths earlier, takes out at room temperature cold slightly (about 15 seconds), with tap water towards workpiece about 10 seconds, after demoulding sound stopped, the organic glass film can take off from glass plate.Organic glass film 12 is attached on the glass plate 14, with length is that 6.5 centimetres glycerine impregnation of fibers bundle places on the organic glass film 12, the glycerine impregnation of fibers bundle of another 1 centimeter length is placed from long fibre Shu Yiduan 0.5 centimeters and vertical with it, the right-angled intersection point is the tie point of two fibrous bundles, long fibrous bundle 2 is used for electrophoretic separation, and staple fibre bundle 7 is used for sample introduction.Fibrous bundle 2 and 7 viscosity because of glycerine are fixed on the organic glass film 12.
(B) embedding and the electricity of fibrous bundle in chip led the making of detection fibers electrophoresis chip
The silicon rubber dimethyl silicone polymer sheet 9 (0.8 millimeters thick) that is the rectangle of chip size size with a middle hollow out is placed on the organic glass film 12 of fibrous bundle, organic glass film 12 is supported by glass plate 13, make fibrous bundle in middle hollow out rectangle, constitute mould cavity 10, above-mentioned pre-gathering solutions is filled with mould cavity, the organic glass cover plate 8 that with another sheet thickness is 1 millimeter again covers on mould cavity 10, compressing and extrude behind the bubble with wavelength is that the uviol lamp of 365 nanometers causes bulk polymerizations by organic glass cover plate 8 irradiation pre-gathering solutions, get fibrous electrophoresis chip base sheet 14 (Fig. 3 (b)), the distance of chip and uviol lamp is 5 centimetres, when temperature is 20 ℃ of left and right sides, need about 30 minutes polymerizables complete.In the one side far away of fibrous bundle in base sheet boring 1,4,5 and 6,2 millimeters in aperture, the boring point is the end of all fibres bundle, and the degree of depth in hole reach make fibrous bundle ends exposed in solution connection holes.Because fibrous bundle is very near from the one side of chip, to prevent from during boring chip is drilled through, drill bit touches fibrous bundle and gets final product.Intrafascicular by with syringe pressurization 70 ℃ hot water being pressed into all fibres a solution connection holes, glycerine is washed out, the base sheet after the boring gets fibrous electrophoresis chip finished product (Fig. 3 (c)) through deburring.Electrolytic solution is injected fibrous bundle by the solution hole on the chip, can carry out electrophoretic separation.Lead the close together (100 micron) of the passage of detection fibers electrophoresis chip from upper surface with the difference of Amperometric Detection Coupled micro-fluidic chip among the embodiment 1 for electricity, the right end that this dispatch from foreign news agency is led the detection fibers electrophoresis need not to cut away.
(C) electricity is led the application of detection fibers electrophoresis chip
The electricity that the present invention makes is led detection fibers electrophoresis chip and 0-3000V high-voltage DC power supply and electricity and is led detector formation chip electrophoresis electricity and lead detection system, successfully is used for K
+, Na
+And Li
+Three kinds of cationic electrophoretic separation, the 0.1mM K of acquisition
+(a), Na
+(b) and Li
+(c) electrophoresis pattern is seen Fig. 5, test condition is: separation voltage is+1500V, sample introduction voltage is+1500V, sample injection time is 1s, and buffer solution is 20mM 2-morpholino b acid (MES)-20mM histidine (pH6.1), and electricity is led detection waveform, and (frequency is 100kHz for sine wave, the P-to-P voltage amplitude is 5V), the range of linearity to the zwitterion of said determination is 0.01-5mM, and detecting lower range is 2-5 μ M, measures 0.1mM K 10 times
+And Na
+The relative standard deviation of peak-to-peak signal be respectively 2.2% and 3.1%, it is wide and reappearance is good, fast efficient to show that this fibrous electrophoresis electricity is led the detection chip range of linearity, just can separate and detect simultaneously three kinds of kations fully in 40 seconds.
The present invention utilizes organic hyaline monomer methyl methacrylate injection molding in-situ polymerization and light-initiated cold process polymerization technique, uses inexpensive fibrous bundle and organic glass sheet to make fibrous electrophoresis chip, and is simple for production and with low cost.The fibrous electrophoresis chip system that the present invention relates to proposes first.The foregoing description shows that this chip has practicality, can be used for the test of actual sample, in fields such as environmental monitoring, clinical diagnosis and food analysis good prospects for application is arranged.
Claims (6)
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096636B (en) * | 2007-07-19 | 2011-12-14 | 复旦大学 | Chip interchangeable microflow control chip proteolysis reactor |
| CN104677972A (en) * | 2015-02-10 | 2015-06-03 | 四川大学 | Constant-speed micro-channel capillary electrophoresis chip |
| CN106053455A (en) * | 2016-05-30 | 2016-10-26 | 江苏微全芯生物科技有限公司 | Thread chip and processing technique and processing device thereof |
| US9610750B2 (en) | 2011-07-20 | 2017-04-04 | Sony Corporation | Composite structure and manufacturing method therefor |
| CN109187648A (en) * | 2018-07-26 | 2019-01-11 | 南方医科大学珠江医院 | The method for improving micro-fluidic chip non-contact conductance method detection sensitivity |
-
2005
- 2005-04-21 CN CN 200510025271 patent/CN1687766A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096636B (en) * | 2007-07-19 | 2011-12-14 | 复旦大学 | Chip interchangeable microflow control chip proteolysis reactor |
| US9610750B2 (en) | 2011-07-20 | 2017-04-04 | Sony Corporation | Composite structure and manufacturing method therefor |
| US10414118B2 (en) | 2011-07-20 | 2019-09-17 | Sony Corporation | Microchip manufactured with thermocompression |
| CN104677972A (en) * | 2015-02-10 | 2015-06-03 | 四川大学 | Constant-speed micro-channel capillary electrophoresis chip |
| CN104677972B (en) * | 2015-02-10 | 2017-02-22 | 四川大学 | Constant-speed micro-channel capillary electrophoresis chip |
| CN106053455A (en) * | 2016-05-30 | 2016-10-26 | 江苏微全芯生物科技有限公司 | Thread chip and processing technique and processing device thereof |
| CN109187648A (en) * | 2018-07-26 | 2019-01-11 | 南方医科大学珠江医院 | The method for improving micro-fluidic chip non-contact conductance method detection sensitivity |
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