CN1660079A - Antibacterial medicine for fishing and its processing technology - Google Patents

Abstract

An antibacterial medicine for fishing contains florfenicol, VC and starch. Clinical tests prove that the florfenicol which is an effective component has lower minimum bacteriostatic concentration, and the antibacterial drug has better prevention and treatment effects on the hemorrhagic septicemia of freshwater fishes caused by aeromonas hydrophila, particularly has good treatment effect at the early stage of disease onset, has better prevention effect on the disease, and has safe use and better stability.

Description

Antibacterial agent use for fishery and processing technique thereof

Technical field

The present invention relates to a kind of antibacterial agent use for fishery and processing technique thereof.

Background technology

Studies show that, the disease of aquatic animal mainly is that bacterial infection causes, as Aeromonas (Aeromonassp.) is that a class extensively distributes at nature, the antibacterial of the various aquatic animals of serious harm, not only cause the cultured fishes hueppe's disease, multiple a large amount of serious epidemic diseases such as shrimp Eriocheir sinensis black gill disease disease, and infected fragrant fish, Carassius auratus, bull frog, Cyprinus carpio, Ctenopharyngodon idellus, Trionyx sinensis Wiegmann, tilapia, Ctenopharyngodon idellus, the aquatic animal that rainbow trout etc. are nearly all, this makes also that in the aquaculture process a large amount of use of need drugs used in aquiculture particularly antibiotic medicine prevent aquatic animal and treat.But in recent years, antibiotics uses kind in aquaculture, dosage and frequency all are improved largely, as sulfonamides, antibiotics such as quinolones have become the main component in the various fisheries drug preparations, particularly some human new drugs such as ofloxacin etc. also use in breeding production in a large number, thereby might cause the antibacterial relevant with human diseases, also might produce drug resistance to these antibiotics, the generation of the residual and Resistant strain of medicine not only directly restricts the sustainable development of culture fishery, influence the international competitiveness of China's aquatic products, the health to human and other animal has constituted potential threat simultaneously.

Summary of the invention

The object of the invention provides antibacterial agent use for fishery and the processing technique thereof that a kind of bacterial disease to aquatic animal has the bacillary secondary infection of preventive and therapeutic effect and prophylaxis of viral diseases.

Technical scheme of the present invention is: a kind of antibacterial agent use for fishery, it contains florfenicol, vitamin C, starch.

A kind of processing technique of antibacterial agent use for fishery, it comprises the following steps,

(1), with starch in the oven dry 2 hours down of 105 ℃～110 ℃ temperature conditions, 40 mesh sieves are crossed in the cooling back;

(2), take by weighing florfenicol and vitamin C by weight proportion;

(3), get and florfenicol and the equiponderant starch of vitamin C, make starch and florfenicol and vitamin C fully be mixed and made into female powder;

(4), female powder is fully mixed with remaining starch.

Florfenicol (Florfenicol) has another name called Florfenicol, is the broad spectrum antibiotic that a kind of new Aquatic product beast of succeeding in developing the late nineteen eighties is herded special-purpose chloromycetin, has the characteristics such as wide, safe and efficient that distribute in has a broad antifungal spectrum, good absorbing, the body.Florfenicol belongs to single fluorine derivative of chloromycetin; mechanism of action is basic identical with chloromycetin (Chlcramphenicol) with antimicrobial spectrum; quite high as bioavailability; the 70S ribosome that can suppress antibacterial; combine peptide for inhibiting acyltransferase, thereby the extension of inhibition peptide chain with the 50S ribosome; disturb the albumen of antibacterial synthetic, Gram-negative and positive bacteria are all had intensive bacteriostasis.But the Resistant strain of chloromycetin produces the mesomeric Acetylase of plasmid usually; this enzyme can make chloromycetin, thiamphenicol the Alpha-Methyl position-acetylization reaction takes place in OH; thereby lose pharmacologically active; but the Alpha-Methyl position of florfenicol-OH replaces by-F, so florfenicol and chloromycetin, thiamphenicol do not have crossing drug resistant.Simultaneously in the chloromycetin structure on the aromatic rings para-position nitro be the main group that causes aplastic anemia, and florfenicol has structurally replaced the O2N group of chloromycetin with H3C-SO2, so does not produce untoward reaction such as aplastic anemia after the medication.Florfenicol is 0.4-0.8ug/ml to the minimal inhibitory concentration of Vibrio anguillarum, tarda, Pasteurella, all lower than antibiotics such as chloromycetin, thiamphenicol, oxytetracyclines, the pseudonucleus Bacillus pasteurii disease that is used for the treatment of amberjack and streptococcicosis, naturally outburst atlantic salmon furunculosis effect remarkable.Oral administration is to amberjack (yellow-tail) pasteurella infection, and the blunt property of eel tarda infects, and Carassius auratus Vibrio anguillarum sexuality is dyed, and salmon Vibrio salmonicida sexuality is dyed good protective action is all arranged, and curative effect surpasses other antibacterials commonly used.The florfenicol intramuscular injection or oral after, absorb rapidly, widely distributed, mean residence time is longer, it is longer to absorb the half-life, the haemoconcentration height can be kept active drug concentration the long period in vivo.As behind the oral 10mg/kg florfenicol of atlantic salmon, pharmacokinetics studies show that the bioavailability of florfenicol reaches 96.5%, peak time is 10.3 hours in the blood, peak concentration is 4ug/ml, and the half-life is 12.2 hours, and apparent volume of distribution is 1.22L/Kg.

High steady vitamin C as the main adjuvant of florfenicol claims ascorbic acid again, belongs to water soluble vitamins.Be that Fish are kept the necessary material of physiological function.After (1965) such as Kitamura confirm by experiment that first the normal growth of Fish need be taken the photograph people's vitamin C, studies show that more vitamin C has important effect to aspects such as the breedings of Fish, growth promoter, disease-resistant and survival rate raisings.Vitamin C keep its normal growth function by Fish and vital movement essential.Vitamin C directly influences the synthetic of collagen protein as the coenzyme of proline hydroxylase in the building-up process of collagen protein, participates in the generation of intercellular substance.The permeability, stimulation coagulation function, the resistant function of increase to infecting that reduce blood capillary are arranged, and participate in function of detoxification.Be used for various acute and chronic infectious disease or other disease to build up resistance, after being ill the auxiliary treatment of convalescent period, wound healing phase.In addition, must have vitamin C to exist in the detoxifcation of the several drugs reaction in vivo, vitamin C also can promote the absorption of ferrum, is of value to hemopoietic function.Vitamin C also participates in intravital redox reaction, participates in the metabolism of folic acid, calcium etc. and synthesizing of parahormone.Aspect disease control of aquatic animal, find that after deliberation vitamin C can influence the healing rate of Fish wound, when being deficient in vitamin C in the feedstuff, before vertebra appears in Oncorhynchi, symptom such as ocular injury; In ditch Nian, also observe vitamin C and can strengthen its pair cell infection.

The present invention compared with prior art has following advantage:

Test report is as follows:

The sample clinical experiment report

Kind of inspection	Clinical trial	Test basis	The Ministry of Agriculture's new fishery Clinical Laboratory standard
				Main content of the test	1, bacteriostatic test.2, to the acute toxicity test of fresh water Macrobrachium nipponensis(de Haan).3, the stability of pharmaceutical preparation is measured.4, the control of freshwater fish hueppe's disease is tested.
Main conclusions	1, bacteriostatic test is 2.2mg/L to the minimal inhibitory concentration of the Aeromonas pathogenic bacterium CL990909-3 bacterial strain of crab.Minimal inhibitory concentration 0.91mg/L to Aeromonas hydrophila pathogenic bacterium ATCC51208 bacterial strain.Minimal inhibitory concentration to freshwater fish bacterial septicemia pathogen Aeromonas hydrophila BSK-10 is 0.91mg/L.Minimal inhibitory concentration to freshwater fish bacterial septicemia pathogen Aeromonas hydrophila DF-30 is 0.91mg/L.Minimal inhibitory concentration to Vibrio anguillarum is 1.1mg/L.Minimal inhibitory concentration to the eel tarda is 2.2mg/L.Minimal inhibitory concentration to fish evil myxobacter is 0.91mg/L.2, to the acute toxicity test using dosage of fresh water Macrobrachium nipponensis(de Haan) 1mg/kg only, and to LD50 〉=500mg/kg of Macrobrachium nipponensis(de Haan), so the use of this product is safe.3, in control test mouthful this product of the filling high dose group to the freshwater fish hueppe's disease, the survival rate that mouth is irritated counteracting toxic substances group Carassius auratus after 4 hours is 90%, and the survival rate of counteracting toxic substances deutostoma filling in 2 hours group Carassius auratus is 80%; In the use group, the survival rate that mouth is irritated counteracting toxic substances group Carassius auratus after 4 hours is 80%, and the survival rate of counteracting toxic substances deutostoma filling in 2 hours group Carassius auratus is 70%; In the low dose group, the survival rate of mouthful filling counteracting toxic substances group Carassius auratus after 4 hours is 60%, and the survival rate of counteracting toxic substances deutostoma filling in 2 hours group Carassius auratus is 40%; The survival rate that mouth is irritated feedstuff counteracting toxic substances group is 10%, and mouthful blank survival rate of organizing of filling feedstuff is 100%.This product has the better prevention effect to the freshwater fish hueppe's disease that is caused by Aeromonas hydrophila, the good curing effect is arranged especially at the initial stage of a disease, and this disease is had preventive effect preferably.4, to measure the response rate of three batches florfenicol be 100.44% to the stability of pharmaceutical preparation, the result shows that the effective ingredient of this product is comparatively stable; Florfenicol is at 40 ± 2 ℃, and relative humidity is that the average content rate of change of preserving after three months under 75 ± 5% conditions is 99.93%.

The bacteriostatic test report

Test strain:

The Aeromonas pathogenic bacterium CL990909-3 of crab

Aeromonas hydrophila pathogenic bacterium ATCC51208

Freshwater fish bacterial septicemia pathogen Aeromonas hydrophila BSK-10

Freshwater fish bacterial septicemia pathogen Aeromonas hydrophila DF-30

Vibrio anguillarum

The eel tarda

Fish evil myxobacter

To the different aquatic animals of the 7 strains mensuration antibacterial, bacteriocidal concentration of causing a disease:

1, culture medium: bacteria culture media gravy peptone culture medium.

2, the preparation of bacterium liquid: pick the test strain of purification with inoculating loop, be inoculated on the gravy peptone culture medium, cultivate after 18 hours, bacterium liquid is diluted to 2,000,000 antibacterial/ml for 28 ℃.

3, assay method: get 10 in sterilization test tube, add sterilization physiology salt 9ml (the 1st pipe), 5ml (2-10 pipe) with the every pipe in sterile working again.The sterile working draws pure chlorine dioxide diluent 1ml to be measured and goes into to put the 1st pipe, draws 5ml behind the mixing and puts into the 2nd pipe, draws 5ml behind the mixing and puts into the 3rd pipe, does to draw 5ml behind the mixing and discard by pipe dilution the 9th pipe with this, and the 10th pipe does not add medicine and compares.

Every pipe adds the good bacterium liquid 0.5ml of dilution, 28 ℃ of cultivations behind the mixing.Pipette 1ml to aseptic culture medium after 24 hours, cultivate perusal after 24 hours, containing the concentration that minimum still is not the test tube contained drug that mixes erosion is minimal inhibitory concentration.Result of the test:

Minimal inhibitory concentration to the Aeromonas pathogenic bacterium CL990909-3 bacterial strain of crab is 2.2mg/L.

Minimal inhibitory concentration 0.91mg/L to Aeromonas hydrophila pathogenic bacterium ATCC51208 bacterial strain.

Minimal inhibitory concentration to freshwater fish bacterial septicemia pathogen Aeromonas hydrophila BSK-10 is 0.91mg/L.

Minimal inhibitory concentration to freshwater fish bacterial septicemia pathogen Aeromonas hydrophila DF-30 is 0.91mg/L.

Minimal inhibitory concentration to Vibrio anguillarum is 1.1mg/L.

Minimal inhibitory concentration to the eel tarda is 2.2mg/L.

Minimal inhibitory concentration to fish evil myxobacter is 0.91mg/L.

Acute toxicity test to the fresh water Macrobrachium nipponensis(de Haan)

In 0.5 square metre of area, dark 0.5 meter net cage, raise 40 of 5.19 ± 1.03g/ fresh water Macrobrachium nipponensis(de Haan)s, support temporarily and carry out acute toxicity test after 7 days, test first three sky and stop feeding.Test is adopted and is mixed the bait oral medication, and the feedstuff feeding amount is 5% of a fresh water Macrobrachium nipponensis(de Haan) body weight.

Before the test, after fresh water Macrobrachium nipponensis(de Haan) feedstuff pulverized, add 20% fish flour and 0.5% feed adhesive, this an amount of antimicrobial drug is admixed in the fresh water Macrobrachium nipponensis(de Haan) feedstuff of having pulverized, add that water is made pellet again and dry under 45 ℃ of conditions.Contain this antimicrobial drug in the medicinal bait and be respectively 2,4,6,8 and 10g/Kg, the throwing amount of raising by 5% is calculated, and then is equivalent to per kilogram fresh water Macrobrachium nipponensis(de Haan) and takes in 100,200,300,400 and the 500mg antimicrobial drug every day.

During test, throw something and feed every day contain this antimicrobial drug pharmaceutical chemistry once, fresh water Macrobrachium nipponensis(de Haan) quantity and be calculated to be motility rate in every net cage of statistics after throwing something and feeding continuously four days.Establish the blank group simultaneously.At least observed three times in per 24 hours, the record death toll is carried out 96 hours observed and recordeds.The water temperature of duration of test is 22 ± 2 ℃, and PH is 7.50.

Result of the test: mortality does not appear in the fresh water Macrobrachium nipponensis(de Haan) of each administration group and matched group, and the survival rate of matched group fresh water Macrobrachium nipponensis(de Haan) is 92.5%, and it is 87.5% that 100mg/Kg forms motility rate; It is 92.5% that 200mg/Kg forms motility rate; It is 87.5% that 300mg/Kg forms motility rate; It is 90.0% that 400mg/Kg forms motility rate; It is 92.5% that 500mg/Kg forms motility rate.Software analysis by statistics, test group and matched group survival rate do not have significant difference, so the result shows: sample is to 96 hours LD50 〉=500mg/Kg body weight of fresh water Macrobrachium nipponensis(de Haan).The results are shown in Table:

Table is to the acute toxicity test of Macrobrachium nipponensis(de Haan)

Drug level (mg/Kg)	Tried shrimp number (only)	96h is tried shrimp survival number (only)	Survival rate (%)
				????100	????40	????35	????87.5
????200	????40	????37	????92.5
				????300	????40	????35	????87.5
????400	????40	????36	????90
				????500	????40	????39	????97.5
Matched group	????40	????37	????92.5

Surface as a result, the use of this product is safe.

Control test to the freshwater fish hueppe's disease

Test strain: the pathogen Aeromonas hydrophila (Aeromonashydrophila that adopts the freshwater fish hueppe's disease, Ah) the BSK-10 bacterial strain (is separated by China Aquatic Science Research Institute's fish diseases research department laboratory, identify through Beijing Institute of Micro-biology of the Chinese Academy of Sciences), be inoculated in the common meat peptone culture fluid, the 25-27 ℃ of bacteria suspension of cultivating 20-24 hour is standby.When carrying out the test of fish body, survey the half lethal dose (LD50) of bacterial strain earlier to fish, inoculate the fish body with the lumbar injection of doubly measuring of LD50 again, the dosage of injection is 4,000 ten thousand/tails.

The material fish of test is a Carassius auratus, and body weight is 50～60 grams, available from the market of farm produce.After earlier in water temperature is 25～28 ℃ indoor cement pit, supporting a week temporarily before the test, and randomly draw five tail Carassius auratuss, blood is carried out antibacterial with meat internal organs official separate, confirm that no Aeromonas hydrophila tests.

One, test method

1, test grouping: with reference to the operation instruction of product: additive capacity is 1% in the feedstuff, and throws something and feeds with 5% daily ration, feeding quantity.Be that per kilogram fish absorption sample is 0.05g.

Test divides three groups, and one group is high dose group, and mouthful filling dosage is 0.500g/Kg, and one group is the using dosage group, and it is 0.050mg/Kg that mouth is irritated dosage, and another group is low dose group, and mouth filling dosage is 0.005mg/Kg.Irritate sample sets with 2 hours deutostomas of 4 * 107 bacterium/tail dosage counteracting toxic substances respectively in each dosage group and mouthful irritate sample counteracting toxic substances group, therapeutic effect and preventive effect of assess sample respectively after 4 hours.Establish mouthful elver feedstuff counteracting toxic substances group of irritating equivalent and mouthful blank group of irritating the equivalent elver simultaneously.

2, test method: at the trial, take by weighing the samples of 0.0625 gram, 0.625 gram and 6.250 grams respectively, admix 30 gram elver feedstuffs, after adding water and making pasty state, standardize solution is in 250 milliliters, and mouthful irritating dosage is that per kilogram fish body weight mouth is irritated 20ml.Make the amount of taking in sample day of Carassius auratus be respectively 0.005g/Kg, 0.050g/Kg, 0.500g/Kg.

In the washing basin that fills 0.3M3 aeration tap water, Carassius auratus 10 tails of 65.3～76.8 grams are raised in every pond, begin test after the foster week temporarily.To make the ciprofloxacin of having prepared suck the 1ml syringe, syringe connects soft rubber tube, emptying air.Carassius auratus is fished for out from the pond, after weighing, from the fish mouth, insert the about 5cm of flexible rubber hose,, pour into 1.31～1.54ml sample respectively, make Carassius auratus take in sample size and be respectively 0.005g/Kg, 0.050g/Kg, 0.500g/Kg according to the body weight of Carassius auratus.The amount of taking in the elver feedstuff is 2.4 gram/Kg, and every day, mouth was irritated once, and mouthful filling is 4 days continuously, establishes the counteracting toxic substances group and the blank group of irritating 2.4 gram/Kg elver feedstuffs by mouth simultaneously.At least observed three times in per 24 hours, the record death toll was carried out observed and recorded 7 days.The duration of test water temperature is 25 ± 1 ℃, and PH is 7.4.

Two, result of the test

Mouth is irritated in the sample high dose group, and the survival rate of mouthful filling counteracting toxic substances group Carassius auratus after 4 hours is 90%, and the survival rate of counteracting toxic substances deutostoma filling in 2 hours group Carassius auratus is 80%; In the use group, the survival rate of mouthful filling counteracting toxic substances group Carassius auratus after 4 hours is 80%, and the survival rate of counteracting toxic substances deutostoma filling in 2 hours group Carassius auratus is 70%; In the low dose group, the survival rate of mouthful filling counteracting toxic substances group Carassius auratus after 4 hours is 60%, and the survival rate of counteracting toxic substances deutostoma filling in 2 hours group Carassius auratus is 40%; The survival rate that mouth is irritated feedstuff counteracting toxic substances group is 10%, and mouthful blank survival rate of organizing of filling feedstuff is 100%.The results are shown in following table:

Table is to having a liking for the prevention effect of freshwater fish hueppe's disease

High dose group

Use the agent group

Low dose group

Mouth is irritated the feedstuff group

The blank group

		Mouth is irritated counteracting toxic substances after 4 hours	2 hours deutostomas of counteracting toxic substances are irritated	Mouth is irritated counteracting toxic substances after 4 hours	2 hours deutostomas of counteracting toxic substances are irritated	Mouth is irritated counteracting toxic substances after 4 hours	2 hours deutostomas of counteracting toxic substances are irritated	???0	???0
										Mouth is irritated sample dose (g)		??0.5	??0.5	??0.050	??0.05	??0.005	??0.005	???0	???0
Injection Ah microbial inoculum amount		??4000	??4000	??4000	??4000	??4000	??4000	???4000	???0
										Test fish number		??10	??10	??10	??10	??10	??10	???10	???10
Death time	First day	??0	??1	??1	??1	??1	??1	???4	???0
										Second day	??1	??1	??1	??2	??1	??1	???3	???0
	The 3rd day	??0	??0	??0	??0	??0	??2	???2	???0
										The 4th day	??0	??0	??0	??0	??2	??2	???0	???0
	The 5th day	??0	??0	??0	??0	??0	??0	???0	???0
										The 6th day	??0	??0	??0	??0	??0	??0	???0	???0
	The 7th day	??0	??0	??0	??0	??0	??0	???0	???0
										Survival rate (%)		??90	??80	??80	??70	??60	??40	???10	???100

The result shows: the freshwater fish hueppe's disease that is caused by Aeromonas hydrophila is had the better prevention effect, particularly the good curing effect is being arranged at the initial stage of a disease, and this disease is had preventive effect preferably.

The drugs used in aquiculture stability of formulation is measured

Florfenicol standard substance purity content is 〉=98.0%

Acetonitrile is a chromatographically pure, and dimethyl formamide is an analytical pure.

One, experimental technique:

Chromatographic condition chromatographic column: C18 (5 μ m, 4mm * 300mm); Mobile phase is acetonitrile: water (4: 6), mobile phase is handled with ultrasonic degas before using.Detect wavelength 223nm, column temperature is a room temperature, flow velocity 1.0ml/ml, application of sample amount 10ul.

Standard curve: precision takes by weighing the about 0.0500mg of florfenicol in the 50ml volumetric flask, add the dissolving of a small amount of dimethyl formamide after. add mobile phase again and be diluted to graticule.Get 2.50,5.00,7.50,10.00,15.00 respectively, 20.00ml is in the 50ml volumetric flask, be diluted to scale with mobile phase, then the concentration of florfenicol be respectively 0.05,0.10,0.1,0.2,0.3,0.4mg/ml, get the 10ul sample introduction respectively, with peak area (Y) concentration (C) is returned, both linear relationships are good, regression equation: C=2837861.25X+6637.41, r=0.9995

The florfenicol standard solution: precision takes by weighing the florfenicol reference substance, is the standard stock solution that solvent is made into 200mg/L with 0.1mol/L NaOH, 4 ℃ of preservations in refrigerator.

Two, result of the test:

1, sample recovery rate is measured: precision takes by weighing sample 5.00g (by 1% production standard, should contain about florfenicol 50mg), places the 250ml beaker, adds dimethyl formamide 20ml.Vibration 10min places tool plug centrifuge tube, and 4 ℃ of high speed centrifuges, the centrifugal 15min of 5000rpm get supernatant, and residue continue to add after dimethyl formamide 20ml stirs, and continues with handling as stated above, and centrifugal back merges supernatant in 50ml volumetric flask standardize solution.Get 10ml extracting solution 10ml then and put in the 100ml volumetric flask, add mobile phase to graticule, with behind the 0.45um membrane filtration, get the 10ul sample introduction, the record peak area also calculates the response rate of florfenicol.See the following form:

Batch	Theoretical amount (μ g/ml)	Actual measured amount (μ g/ml)	The response rate (%)	Average recovery rate (%)
					??20030705	????10.00	????10.15	?101.5	100.44
????10.00	????10.12	?101.2
			??20030715	????10.00		????9.937	?99.37
????10.00	????9.858	?98.58
				??20030809	????10.00	????9.961	?99.61
????10.00	????10.24	?102.4

2, the example pharmaceuticals preparation stability is investigated: the pharmaceutical preparation study on the stability carries out with reference to the relevant accelerated test of Ministry of Public Health bureau of drug administration " new drug preclinical study guideline compilation ".

Because the host of this product is florfenicol, and adjuvant is high steady vitamin C and starch, because can not produce degradation reactions such as oxidation, hydrolysis between host and adjuvant, therefore three batches sample is sub-packed in the Brown Glass Brown glass bottles and jars only, places 40 ± 2 ℃, relative humidity is in 75 ± 5% the incubator, every sampling in 5,15,30,60,90 days, the method of pressing in the sample recovery rate mensuration is extracted florfenicol in the sample, and the content with florfenicol in the high effective liquid chromatography for measuring preparation the results are shown in following table:

Batch	Theoretical amount (μ g/ml)	Actual measured amount (μ g/ml)					The 90d average content
								??5d	??15d	??30d	??60d	??90d
		??20030705	????10.00	??10.12	??9.98	??9.95							??9.96	??9.95	????9.93
????10.00	??9.98						??10.03	??10.01	??9.96	??9.96
			??20030715	????10.00	??9.98	??9.98					??9.98	??9.96	??9.96
????10.00	??9.97	??9.98					??9.94	??9.87	??9.82
				??20030809	????10.00	??9.98				??9.96	??9.97	??9.96	??9.90
????10.00	??10.90	??9.97	??9.97				??9.94	??9.88

The content of florfenicol in the time of 90 days reduces 0.7% in the preparation.Accelerated test result shows that this pharmaceutical preparation stability is better, and it is 2 years that effect duration orders temporarily.

Three, main conclusions: the response rate of three batches florfenicol is 100.44%, and the result shows that the effective ingredient of this product is comparatively stable; Florfenicol is at 40 ± 2 ℃, and relative humidity is that the average content rate of change of preserving after three months under 75 ± 5% conditions is 99.93%.Therefore comparatively stable.

The specific embodiment

A kind of antibacterial agent use for fishery, it contains florfenicol, vitamin C, starch.

The weight proportion of described florfenicol is 0.5%～1.5%, and described ascorbic weight proportion is 2%～3%, and the weight proportion of described starch is 96%～97%.

The processing technique of this antibacterial agent use for fishery comprises the following steps:

(1), with starch in the oven dry 2 hours down of 105 ℃～110 ℃ temperature conditions, 40 mesh sieves are crossed in the cooling back;

(2), take by weighing florfenicol and vitamin C by weight proportion;

(3), get and florfenicol and the equiponderant starch of vitamin C, make starch and florfenicol and vitamin C fully be mixed and made into female powder;

(4), female powder is fully mixed with remaining starch.

The purity content of the florfenicol of being got in the step (2) is greater than 98%.

The major advantage of this product can be by the concrete proof of test, the minimal inhibitory concentration of the florfenicol that it is contained is less, safe in utilization, the freshwater fish hueppe's disease that is caused by Aeromonas hydrophila there is the better prevention effect, the good curing effect is arranged especially at the initial stage of a disease, and this disease had preventive effect preferably, and the stability of the effective ingredient of this antibacterial medicine preparation better.

On the flesh of fish, be difficult for adherent situation at medicine,, can solve the adhesiveness of medicine the starch of this product α starch.According to the production demand, processing shrimp, the grains dedicated bait of Eriocheir sinensis use for the raiser in addition, and good effect has all been received in its control to shrimp Eriocheir sinensis bacterial diseases such as tremble disease, black gill disease disease, rotten shell disease, enteritis.

Claims (5)

1, a kind of antibacterial agent use for fishery is characterized in that: it contains florfenicol, vitamin C, starch.

2, antibacterial agent use for fishery according to claim 1 is characterized in that: the weight proportion of described florfenicol is 0.5%～1.5%, and described ascorbic weight proportion is 2%～3%, and the weight proportion of described starch is 96%～97%.

3, antibacterial agent use for fishery according to claim 1 is characterized in that: described starch is α starch.

4, a kind of processing technique of antibacterial agent use for fishery is characterized in that: it comprises the following steps,

(1), with starch in the oven dry 2 hours down of 105 ℃～110 ℃ temperature conditions, 40 mesh sieves are crossed in the cooling back;

(2), take by weighing florfenicol and vitamin C by weight proportion;

(3), get the starch that equates with florfenicol and ascorbic gross weight, make starch and florfenicol and vitamin C fully be mixed and made into female powder;

(4), female powder is fully mixed with remaining starch.

5, the processing technique of antibacterial agent use for fishery according to claim 4 is characterized in that: the purity content of the florfenicol of being got in the step (2) is more than or equal to 98%.