CN1504578A - biological cell detection method and device - Google Patents

Abstract

A method and device for detecting biological cells, which utilizes a traditional cell shelf, wherein the cell shelf is provided with a plurality of arranged through holes, and the through holes are provided with two electrodes made of metal materials; when the cell falls into the perforation, the cell body contacts with the two electrodes, and the cell body has the conductive characteristics of impedance, inductance or capacitance, so that when the two electrodes are electrified with current or voltage, the current or electric field flows from the electrode to the other electrode, and when the cell is electrified with the electrical characteristics, the cell can present whether the cell exists or not or can show physical reaction.

Description

Biological cell detection method and device

technical field

The present invention relates to a method and device for detecting biological cells, especially to apply current to animal and plant cells by using physical characteristics, plus the impedance, inductance and capacitance characteristics of the cells themselves, after the cell body is passed through the current or electric field , you can know whether the cells exist on multiple perforations or make the cells present a physical response.

Background technique

Biotechnology is a technology that uses biological cells or their metabolites to manufacture products, or improves animals, plants, microorganisms and related products to improve the quality of human life. The industrial scope of this technology application includes medicine, food, specialization, environmental protection, ocean, energy and agriculture. In order to meet the needs of biotechnology research and development, such as gene map sequencing, the biochemical reactions in the research process need to be large and rapid, so "biochips" came into being. The so-called biochip is the integration of functional biomaterials and micro-electromechanical technology. Micro-electromechanical technology is expected to provide micro-planning and design of chips, and can provide multiple micro-regions in a small area as reaction sites for functional biomaterials.

In the past, when biological cells were detected on biochips, chemical methods were used to detect biological cells directly into the perforated cell carrier (perforated cell carrier), and then dropped into chemical agents, adding fluorescent agents, and at the same time Observing the response of biological cells using an optical architecture. This kind of detection method using chemical method not only has trivial detection steps, but also uses more peripheral equipment, and when using chemical method for detection, the chemical agent is easy to damage biological cell tissue, resulting in a short storage time of biological cells.

Contents of the invention

The main purpose of the present invention is to solve the defects of the above-mentioned prior art and avoid the existence of defects. The present invention uses a new detection method and device to change the traditional chemical method to understand the characteristics (physical properties) of its cells (animal and plant cells) . This new method uses a physical method for some cells with inductive or capacitive properties. It is not necessary to add fluorescent agents during detection, so that the physical properties of the cells can be known, and at the same time, the optical structure can be saved. Thereby making the detection of biological cells more convenient and easy.

In order to achieve the above-mentioned purpose, this new method is to use the traditional cell storage rack, which has a plurality of perforations (holes) arranged on the cell storage rack, and two electrodes made of metal materials are arranged in the perforation holes, and the layout of the two electrodes consists of The inside of the perforation extends from the outside of the perforation. When the cell falls into the aforementioned perforation, the cell body will be in contact with the two electrodes. In addition, the cell body has the conductive characteristics of impedance, inductance or capacitance, so there is current or current flowing between the two electrodes. When the voltage is applied, the current or electric field will flow from the electrode through the cell to the other electrode. When the cell flow has electrical characteristics, it is not necessary to add a fluorescent agent to know whether the cell exists on multiple perforations or to let the cell Present the physical response to achieve the purpose of detecting cells by physical methods.

Description of drawings

The detailed content and technical description of the present invention are described as follows in conjunction with the accompanying drawings.

Figure 1-1 is a schematic diagram of the cell detection rack of the present invention.

Figure 1-2 is a partially enlarged schematic diagram of Figure 1-1.

Fig. 1-3 is a schematic cross-sectional view at position A-A of Fig. 1-2.

Fig. 2 is a schematic diagram of the second embodiment of the present invention.

Fig. 3 is a schematic diagram of a third embodiment of the present invention.

Explanation of the numbers in the drawings

Cell rack........1

Perforation .................11

Two electrodes...................12, 12’

cells .................. 2

Heating device................3

Sensing device................4

Detailed ways

Referring to Figures 1-1, 1-2, and 1-3, they are schematic diagrams of the cell detection rack and partial enlargements of the present invention. As shown in the figure: the cell detection method and device of the present invention mainly use new detection methods and devices to change the traditional chemical method to understand the characteristics (physical properties) of its cells (animal and plant cells). This new method uses a physical method for some cells with inductive or capacitive properties. It is not necessary to add fluorescent agents during detection, so that the physical properties of the cells can be known, and at the same time, the optical structure can be saved. Thereby making the detection of biological cells more convenient and easy.

The new method mentioned above utilizes a traditional cell carrier (perforated cell carrier) 1, which has a plurality of perforated holes (holes) 11 arranged on the cell carrier 1, and the inside of the perforated cells 11 is in a state of tapering from top to bottom. In the perforation 11, there are two electrodes 12, 12' made of metal material. The arrangement of the two electrodes 12, 12' extends from the inside of the perforation 11 to the outside of the perforation 11. When the cell 2 falls into the aforementioned perforation 11, The cell 2 body will be close to or in contact with the two electrodes 12, 12', and the cell 2 body has the conductive properties of impedance, inductance or capacitance, so a direct or alternating current or voltage is passed through the two electrodes 12, 12' , the current or electric field will flow from the electrode 12 through the cell 2 to the other electrode 12'. When the cell 2 has electrical characteristics, it is not necessary to add a fluorescent agent to detect whether the cell 2 exists or to make the cell 2 appear. Physical property response, in order to achieve the purpose of detecting cell 2 by physical method.

At the same time, by using the above-mentioned physical method of electrical characteristics to detect the cell 2, the physical property response of the cell 2 can be known without the aid of the optical structure, which can greatly reduce the use of the optical structure. Moreover, when the cell 2 is detected by a physical method using electrical characteristics, the tissue of the cell 2 will not be damaged, so that the storage time of the cell 2 is longer.

Referring to Fig. 2, it is a schematic diagram of the second embodiment of the present invention. As shown in the figure: In addition to adding two electrodes 12, 12' to the perforation 11 of the traditional cell rack 1 to physically detect the electrical characteristics of the biological cells 2, the present invention can also be formed with the electrodes 12, 12'. There is a heating device (heating wire) 3 inside the perforation 11, after the cells 2 fall into the perforation 11 of the cell rack 1, in addition to the electrical characteristics that can apply current to the cells 2, the cells 2 are also heated at the same time, so that Cell 2 has a response to heating in addition to the response to current flow.

Referring to FIG. 3 , it is a schematic diagram of a third embodiment of the present invention. As shown in the figure: this embodiment is roughly the same as the above-mentioned embodiment, the difference is that the heating device 3 is arranged on the periphery of the perforation 11, and a sensing device 4 is also added on the cell rack 1 at the same time. The device 4 is used to sense the heating temperature of the cells 2 to avoid necrosis of the cells 2 caused by overheating, and can effectively detect the cells 2 .

Further, the heating device mentioned above can be not only an electric heating wire, but also an external heating device to heat the cells 2 on the cell rack 1 .

Furthermore, besides being arranged on the cell rack 1 , the sensing device can also be constituted by external sensing devices.

The foregoing are only preferred embodiments of the present invention, and are not intended to limit the implementation scope of the present invention. That is, all equivalent changes and modifications made according to the claims of the present invention are covered by the patent scope of the present invention.

Claims (10)

1. A cell detection device, which is a device for detecting cells using physical characteristics, is characterized in that the device includes: A cell rack (1) for accommodating cells (2), which has a plurality of arranged perforations (11); An electrode (12, 12') that is arranged on the above-mentioned cell rack (1) and extends inside the perforation (11); Thereby, when the biological cell (2) falls into the perforation (11) and is close to or in contact with the electrode (12, 12'), when the electrode (12, 12') is supplied with current or voltage, the current or electric field will flow through the biological The cells (2) can know whether the cells (2) exist on the plurality of arranged perforations (11) or make the biological cells (2) present a physical response. 2. The cell detection device according to claim 1, the electrode (12, 12') has a two-electrode characteristic, so as to pass a direct or alternating current or voltage. 3. The cell detection device according to claim 1, which is further provided with a heating device (3) arranged on the cell rack (1). 4. The cell detection device according to claim 3, the heating device (3) can be arranged on the cell rack (1) and extend into the perforation (11). 5. The cell detection device according to claim 3, the heating device (3) can be a heating wire. 6. The cell detection device according to claim 3, the heating device (3) can be formed by an external heating device. 7. The cell detection device according to claim 1, further comprising a sensing device (4) arranged on the cell rack (1). 8. The cell detection device according to claim 7, the sensing device (4) is a temperature sensing device. 9. The cell detection device according to claim 7, the sensing device (4) can be constituted by an external sensing device. 10. A method for detecting cells (2) using the cell detection device according to claim 1 using physical characteristics, the method comprising: a) Using the cell rack (1), the cell rack (1) has a plurality of perforations (11), and the perforations (11) are provided with two electrodes (12, 12') made of metal materials; b) When the cell (2) falls into the aforementioned perforation (11), the cell (2) body will be close to or in contact with the two electrodes (12, 12'), and the cell (2) body has impedance, inductance or the electrical conductivity of the capacitive substance, so when the two electrodes (12, 12') are connected with current, the current or electric field will flow from the electrode (12, 12') through the cell (2) to the other electrode (12, 12' ), when the cell (2) has electrical characteristics in circulation, the cell (2) can show a physical response.

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