CN1563373A - Method for raising stability for preserving catalase prepared by microbiological method - Google Patents

Abstract

The invention discloses a method for improving the storage stability of catalase prepared by a microorganism method, and relates to a biological enzyme used in clean production in the textile industry. The present invention adopts a strain Thermoascus aurantiacus IFO9862 purchased from Japan NBRC (formerly Japan "Sakae Fermentation Research Institute") as the starting strain, the catalase prepared by liquid submerged fermentation, and ultrafiltration Add a certain amount of stabilizer sodium chloride, gelatin, or glycerol after concentration, store at 40°C for 60 days, and the enzyme activity retention rate is above 90%. The stabilizer involved in the invention is cheap, can solve the problem that the liquid catalase is easily inactivated, and significantly improves the stability of the enzyme.

Description

A method for improving the storage stability of catalase prepared by microbial method

technical field

The invention relates to a method for improving the storage stability of catalase prepared by a microorganism method. It relates to a biological enzyme used in the clean production of the textile industry.

Background technique

Hydrogen peroxide is today the most commonly used bleaching agent for all natural fibers of vegetable or animal origin, and the process is ecologically acceptable and economically viable. Therefore, hydrogen peroxide bleaching or scouring "chlorine-free bleaching" process is becoming more and more widely used. However, the presence of hydrogen peroxide in the dyebath can cause fading of reactive dyes that are sensitive to oxidation. It has been shown that even small changes in dye molecules can lead to loss of color. Due to strict quality requirements, even slight color changes in dyed fabrics are not commercially acceptable. Therefore, once the bleaching process is over, in order to ensure the safety of subsequent dyeing, the residual hydrogen peroxide after oxygen bleaching must be removed to avoid problems in the subsequent dyeing process. The traditional process is unsatisfactory: if it needs to heat up and wash many times, it may bring in toxic and refractory substances. The reaction rate of catalase is very fast: under optimal conditions, each mole of catalase can decompose 5 million moles of hydrogen peroxide in one minute. Therefore, the use of catalase in the dyeing and finishing process to replace multiple cleanings and chemical reducing agents to remove residual H ₂ O ₂ in bleaching solutions has great economic and environmental advantages. However, due to the easy inactivation of liquid catalase, its wide application is limited to some extent.

Contents of the invention

The purpose of the present invention is to provide a stabilizer with better preservation effect for catalase. The use of the stabilizer of the invention can solve the problem that the liquid catalase is easily inactivated, and significantly improves the stability of the enzyme.

The technical scheme of the present invention adopts a strain Thermoascus aurantiacus (Thermoascus aurantiacus) IFO9862 purchased from Japan's NBRC (formerly Japan's "Sakae Fermentation Research Institute") as the starting strain, and the catalase prepared by liquid submerged fermentation, The enzyme activity is 2000U/mL, the fermentation broth is suction filtered to remove the bacteria, and then concentrated 10 times by ultrafiltration. L, or glycerol 80-100g/L, stored at 40°C for 60 days, the enzyme activity retention rate is over 90%.

Beneficial effects of the present invention:

In terms of cost: the stabilizers involved are low in price and moderate in dosage.

In terms of product quality: due to the addition of a stabilizer suitable for liquid catalase storage, the stability of the enzyme has been greatly improved, and the enzyme activity retention rate is still above 90% when stored at 40°C for 60 days.

Detailed ways

Example 1

The catalase prepared by liquid submerged fermentation was removed by suction filtration, and the enzyme activity was 2000U/mL. It was stored at 40°C for 30d and 60d, and the enzyme activity retention rates were 21.3% and 2.4%, respectively. After it was concentrated by ultrafiltration for 10 times, the enzyme activity was 20000U/mL, and stored at 40°C for 30d and 60d, the enzyme activity retention rates were 74.7% and 9.6%, respectively.

Example 2

The stabilizer sodium chloride 20mmol/L was added to the ultrafiltered enzyme liquid, and it was stored at 40°C for 60 days, and the enzyme activity retention rate was 94.0%.

Example 3

Add stabilizer gelatin 0.5g/L to the enzyme solution after ultrafiltration, store at 40°C for 60 days, and the enzyme activity retention rate is 93.9%.

Example 4

The stabilizer glycerol 90g/L was added to the enzyme solution after ultrafiltration, and it was stored at 40°C for 60 days, and the enzyme activity retention rate was 90.8%.

Claims (1)

1. method that improves the catalase storage stability of microbial method preparation, it is characterized in that adopting available from golden yellow thermophilic ascomycete (Thermoascus aurantiacus) IFO9862 of the strain of Japanese NBRC as starting strain, catalase through the liquid submerged fermentation preparation, enzyme is lived and is 2000U/mL, the fermented liquid suction filtration removed thalline after 10 times of ultrafiltration and concentration, enzyme is lived and is 20000U/mL, add stablizer sodium-chlor 15-25mmol/L respectively, or gelatin 0.4-0.6g/L, or glycerine 80-100g/L, preserve 60d at 40 ℃, its enzyme is lived retention rate more than 90%.