CN1453585A - Stirring configuration for use on biochips - Google Patents
Stirring configuration for use on biochips Download PDFInfo
- Publication number
- CN1453585A CN1453585A CN 02118329 CN02118329A CN1453585A CN 1453585 A CN1453585 A CN 1453585A CN 02118329 CN02118329 CN 02118329 CN 02118329 A CN02118329 A CN 02118329A CN 1453585 A CN1453585 A CN 1453585A
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- biochip
- concussion
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- stirring structure
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- 238000000018 DNA microarray Methods 0.000 title claims abstract description 42
- 238000003756 stirring Methods 0.000 title claims abstract description 40
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- 230000009514 concussion Effects 0.000 claims description 27
- 239000012190 activator Substances 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 239000000919 ceramic Substances 0.000 claims description 5
- 239000012488 sample solution Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
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- 150000001720 carbohydrates Chemical class 0.000 claims description 2
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 2
- 150000002894 organic compounds Chemical class 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 235000014633 carbohydrates Nutrition 0.000 claims 1
- 239000012472 biological sample Substances 0.000 abstract description 9
- 239000000523 sample Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 description 5
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- 239000007788 liquid Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
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- 238000004140 cleaning Methods 0.000 description 2
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- 238000012360 testing method Methods 0.000 description 2
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
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- 239000000463 material Substances 0.000 description 1
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- 239000002547 new drug Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A stirring structure used on a biochip comprises a vibration unit capable of controlling vibration frequency, a transmission part driven by the vibration of the vibration unit, and a stirring unit connected with the transmission part and positioned above the biochip at a specific distance, so that the vibration unit generates vibration with specific frequency to a biological sample solution to be tested to drive the transmission part to drive the stirring unit, the stirring unit is used for stirring and mixing the vibration with specific frequency to the biological sample solution, a Target in the biological sample solution is enabled to interact with a biological Probe (Probe) on the biochip, and other non-acted Interferent (Interferent) is cleaned and separated, thus, the biochip stirring structure capable of effectively mixing and separating the biological sample solution is formed.
Description
Technical field
The present invention relates to a kind of stirring structure that is used on the biochip, refer to a kind of stirring structure that can effectively mix with separating bio chip solid-liquid interface biological specimen solution especially.
Background technology
The Human Genome Project that nineteen ninety begins (Human Genome Project) is formally finished DNA in the human gene chromosome (DNA) ordering first draft in June, 2000 by U.S. Sai Leila (Celera) company and supranational institution's " Human Genome Project ", finishes the decoding of whole human genes before 2002.The plan of the new milestone of biomedical revolution of this 21 century is finished, and can match in excellence or beauty with the plan of landing on the moon of generation nineteen sixty.
Finish along with the human genome collection of illustrative plates is about to sequencing, scientist's next problem is to understand meaning and its mutual relationship of tens thousand of gene representatives, and the functional study of aleuroplast (Proteomics).And biochip solves the best sharp weapon of this complicated difficult problem just.The principal feature of biochip technology is that it analyzes the accuracy height, analysis speed is fast, and the corpse or other object for laboratory examination and chemical testing sample and the reagent of required use are few, and once test the experimental data that just can obtain globality (parallelization).Therefore, but the range of application of biochip has contained gene functional research, new drug development, clinical examination, bacterial screening, environment control or the like.
The principle of biochip is to utilize microelectronics, industrial technologies such as micromechanics, (main source has organic compound with bioprobe (Probe), nucleic acid, protein, carbohydrate, cell or tissues etc.) (its material can be glass to be fixed on the substrate (Substrate) of thumbnail size, silicon chip, plastics etc.) on, the biological sample solution (blood that contains object (Target) with institute's desire detection, urine, body fluid or solution) on chip, mix, owing to object has specificity with bioprobe on the corresponding chip and can interact (mutually combine or react to each other) causes that signal (comprises optics, piezoelectricity, galvanochemistry, energy signal) change, or in further detachment process, the object of combination cleaned with unconjugated chaff interference (Interferent) and separated, utilize optics (fluorescent afterwards, the ferment colorimetric) or bioprobe-object compound is carried out optics to various marker methods such as radiating matter or activity detects quantitatively and data analysis, so just can obtain the analytical information of detection of biological sample.
But, conventional hybrid and separation steps are very simple, be that the chip that adds biological sample solution is placed a side, after treating the schedule time, directly the water flushing is cleaned the object of combination and is separated with other chaff interference, coarse like this method is easy to because the adding of the error of predetermined mix time and cleaning liquid causes the puzzlement in the quantitative test.
Summary of the invention
Fundamental purpose of the present invention, be to solve the aforesaid drawbacks, avoid the existence of defective, the present invention utilizes piezoelectric ceramics (Piezoelectic ceramic) converter (Transducer) that the voltage concussion can be converted into the mechanicalness vibrations to create stirring structure on the biochip, be applied in the analyzing biochips step and (comprise immobilization, clean, interact) in, its structure includes the concussion unit of may command concussion frequency, one is shaken the driving section of unit transmission concussion, one agitating unit that is connected and driven by the driving section with the driving section, be assembled with more than one stirring rod at this agitating unit, the concussion that makes the concussion unit produce characteristic frequency, drive agitating unit by the driving section, make the stirring rod that is positioned at specific range on the biochip produce vibrations, and object specific in the bioprobe that is fixed on the biochip and the solution is interacted, and the object that will not act on is separated, by accurate control concussion frequency, the time of amplitude of vibration and concussion, reaching increases the rapid of biological test, and susceptibility, the purpose of simplification and economy.
Relevant detailed description of the present invention and technology contents, existing as follows with regard to accompanying drawings:
Description of drawings
Fig. 1 is a structural representation of the present invention;
Fig. 2 is a diagrammatic cross-section of the present invention;
Fig. 3 is a user mode synoptic diagram of the present invention;
Fig. 4 figure is a horizontal balance synoptic diagram of the present invention;
Fig. 5 is a vertically-acting synoptic diagram of the present invention;
Fig. 6 is configured in one piece figure of the present invention.
Embodiment
See also Fig. 1, it is structural representation of the present invention, as shown in the figure, its structure of the present invention mainly includes the concussion unit 1 of may command concussion frequency, one is shaken the driving section 2 of unit 1 concussion transmission, one agitating unit 3 that is connected in driving section 2 and driven by driving section 2, between driving section 2 and agitating unit 3, fix (or can integrally formedly make with agitating unit 3 this driving section 2) by a connection piece 4, wherein, this agitating unit 3 is assembled with more than one stirring rod 31, and connect a stirring ring 32 that increases the vibrations scope at this stirring rod 31 free ends, the concussion that the biological specimen solution 5 that 1 pair of unit of concussion will be checked produces characteristic frequency, drive agitating unit 3 to drive driving section 2, the vibrations that make agitating unit 3 specific range position on biological specimen solution 5 produce characteristic frequency are stirred, mix, and object 51 and the bioprobe on the biochip 61 in the biological specimen solution sample 5 are interacted, and other chaff interference 52 that will not act on is separated, like this, constitute biochip 6 stirring structures of a kind of effective mixing and separating bio sample solution 5.
See also Fig. 2, it is diagrammatic cross-section of the present invention, concussion of the present invention unit 1 mainly includes a piezo-activator 11 (Peizoelectric actuator), this piezo-activator 11 is to be formed by a plurality of piezoelectric ceramics assemblies 111 (Peizoelectric ceramicelements) storehouse, wherein, each piezoelectric ceramics assembly 111 is handled (Poling process) and is had polarity through hyperpolarization, can produce the mechanicalness vibrations by the phase transition that the voltage concussion is caused, in addition, be coated with a housing 12 with shield effectiveness in above-mentioned piezo-activator 11 outsides, and connect at this piezo-activator 11 1 ends and always to put on the driving section 2 of stating housing 12, by this driving section 2 to connect and to drive above-mentioned agitating unit 3.
During use, earlier biological specimen solution 5 and corresponding biochip 6 are ready to, its biological specimen solution 5 contains object 51 and the chaff interference 52 that desire detects, in addition, its biochip 6 is the substrates that are fixed with bioprobe 61 on it, this bioprobe 61 can carry out the affinity combination or carry out the catalytic reaction with corresponding object 51, afterwards, biochip 6 is inserted agitating unit of the present invention 3 belows, start the present invention and drop to chip top ad-hoc location and begin concussion, can carry out biological specimen solution 5 and mix with characteristic frequency, interact and the cleaning separation steps.
Its mixing and separating process, see also Fig. 3,4,5, it is user mode of the present invention, horizontal balance and vertically-acting synoptic diagram, as shown in the figure, the biochip 6 that is added with biological specimen solution 5 is outward placed the below of agitating unit 3 of the present invention, and with the corresponding stirring rod 31 of the regional alignment that has bioprobe 61 on the biochip 6, and because the stirring ring 32 of stirring rod 31 lower ends can hold biological sample liquid 5, when starting concussion unit 1 and produce characteristic frequency vibrations at biological specimen solution 5, this agitating unit 3 is subjected to driving section 2 and drives, the solid-liquid interface that its stirring ring 32 that is connected in agitating unit 3 lower ends is promptly formed by bioprobe 61 and biological specimen solution 5 on biochip 6 begins disturbance, its perturbation direction can perpendicular or parallelly act on this biological specimen solution 5, because bioprobe 61 has single corresponding characteristics with object 51, and the energy that the energy that its agitating unit 3 forcing frequencies are produced just combines with object 51 less than bioprobe 61, make the disturbance that is produced when stirring not only can promote bioprobe 61 to combine with object 51, and clean, other chaff interference 52 on the separating bio probe 61 is in order to reach the effect of effective mixing and separating bio sample solution 5.
See also Fig. 6, be configured in one piece figure of the present invention, the stirring structure of biochip 6 of the present invention is arranged on one to have on the analyser of biochip 6 of chip pocket 7, between the present invention and chip pocket 7, be provided with a holder 8, and on this holder 8, offer the perforation 9 of passing for corresponding stirring rod 31, and the present invention and holder 8 are available for users to adjust up and down, with making biochip 6 insert chip pocket 7, and make things convenient for the user to add detection of biological sample solution 5.
Claims (9)
1. stirring structure that is used on the biochip (6), it is characterized in that: described stirring structure includes the concussion unit (1) of may command concussion frequency, one is shaken the driving section (2) of unit (1) concussion transmission, one is connected and is positioned at the agitating unit (3) of biochip (6) top specific range with driving section (2), make concussion unit (1) produce the concussion of characteristic frequency to the biological specimen solution (5) that will check, drive agitating unit (3) to drive driving section (2), agitating unit (3) is stirred the vibrations that biological specimen solution (5) produces characteristic frequency, mix, and the object (51) in the biological specimen solution (5) is interacted with biochip (6), and other chaff interference (52) that will not act on is cleaned separation, like this, constitute biochip (6) stirring structure of a kind of effective mixing and separating bio sample solution (5).
2. the stirring structure of biochip according to claim 1 (6) is characterized in that, different concussion frequencies can be adjusted corresponding to different biological specimen solution (5) such as organic compound, nucleic acid, protein, carbohydrates in described concussion unit (1).
3. the stirring structure of biochip according to claim 1 (6) is characterized in that, described agitating unit (3) can carry out concussion perpendicular to biochip (6) surface direction corresponding to biological specimen solution (5).
4. the stirring structure of biochip according to claim 1 (6) is characterized in that, described agitating unit (3) can be parallel to the concussion of biochip (6) surface direction corresponding to biological specimen solution (5).
5. the stirring structure of biochip according to claim 1 (6) is characterized in that, concussion unit (1) includes a piezo-activator, and one coats the housing (12) that described piezo-activator has shield effectiveness.
6. the stirring structure of biochip according to claim 5 (6) is characterized in that, described piezo-activator is to be formed by a plurality of piezoelectric ceramics assemblies (111) storehouse.
7. the stirring structure of biochip according to claim 1 (6) is characterized in that, described agitating unit (3) is connected with at least more than one stirring rod (31).
8. the stirring structure of biochip according to claim 7 (6) is characterized in that, the bottom configuration one stirring ring (32) perpendicular to stirring rod (31) of described stirring rod (31) is in order to increase the stirring scope.
9. the stirring structure of biochip according to claim 1 (6) is characterized in that, described biochip (6) is gone up point and is provided with the bioprobe (61) that at least more than one acts on above-mentioned object (51).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02118329 CN1206536C (en) | 2002-04-24 | 2002-04-24 | Stirring configuration for use on biochips |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02118329 CN1206536C (en) | 2002-04-24 | 2002-04-24 | Stirring configuration for use on biochips |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1453585A true CN1453585A (en) | 2003-11-05 |
| CN1206536C CN1206536C (en) | 2005-06-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02118329 Expired - Fee Related CN1206536C (en) | 2002-04-24 | 2002-04-24 | Stirring configuration for use on biochips |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1206536C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019010677A1 (en) * | 2017-07-13 | 2019-01-17 | 王俊超 | Sound wave homogenising module and biological sample preparation system |
-
2002
- 2002-04-24 CN CN 02118329 patent/CN1206536C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019010677A1 (en) * | 2017-07-13 | 2019-01-17 | 王俊超 | Sound wave homogenising module and biological sample preparation system |
| CN110914405A (en) * | 2017-07-13 | 2020-03-24 | 创茂生技股份有限公司 | Sound wave homogeneity module and biological sample preparation system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1206536C (en) | 2005-06-15 |
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