CN1333247C - Buffered split flow device in use for electrophoresis of free stream - Google Patents
Buffered split flow device in use for electrophoresis of free stream Download PDFInfo
- Publication number
- CN1333247C CN1333247C CNB200510024412XA CN200510024412A CN1333247C CN 1333247 C CN1333247 C CN 1333247C CN B200510024412X A CNB200510024412X A CN B200510024412XA CN 200510024412 A CN200510024412 A CN 200510024412A CN 1333247 C CN1333247 C CN 1333247C
- Authority
- CN
- China
- Prior art keywords
- cavity
- antifluctuator
- air cushion
- chamber
- flow arrangement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
本发明涉及一种用于自由流电泳的缓冲分流装置，缓冲器腔体由下部储液室和上部气垫室组成，连通电解质/样品溶液的输入管经输入管接口连通缓冲器腔体，缓冲器腔体经输出管与输出管接口连通自由流电泳分离室，气垫调节注射器经气垫调节阀连通缓冲器腔体，装置上部安装水平仪，底部设置水平调节旋纽。本发明中的储液室和气垫室在同一缓冲器腔体内，气垫室中的气垫能有效缓冲输液泵导致的压力-流速脉冲，输液泵输送到缓冲分流装置的液体经输出管接口、输出管形成多达几十到几百条的稳定均匀的液体输送模式。本发明可应用于自由流电泳中液体输送或样品进样，显著降低自由流电泳对高精度高稳定多道泵的依赖。
The invention relates to a buffering and splitting device for free-flow electrophoresis. The buffer cavity is composed of a lower liquid storage chamber and an upper air cushion chamber, and an input tube connected to electrolyte/sample solution is connected to the buffer cavity through an input tube interface. The chamber is connected to the free-flow electrophoresis separation chamber through the outlet pipe and the outlet pipe interface, and the air cushion adjustment syringe is connected to the buffer chamber through the air cushion adjustment valve. A level is installed on the upper part of the device, and a level adjustment knob is installed on the bottom. The liquid storage chamber and the air cushion chamber in the present invention are in the same buffer cavity, and the air cushion in the air cushion chamber can effectively buffer the pressure-flow velocity pulse caused by the infusion pump, and the liquid delivered by the infusion pump to the buffering and diverting device passes through the output pipe interface, the output pipe A stable and uniform liquid delivery pattern of dozens to hundreds of lines is formed. The invention can be applied to liquid delivery or sample injection in free-flow electrophoresis, and significantly reduces the dependence of free-flow electrophoresis on high-precision and high-stability multi-channel pumps.
Description
Technical field
The present invention relates to a kind ofly be used for free stream cataphoresis (Free-Flow Electrophoresis, buffering part flow arrangement FFE) are used for the free stream cataphoresis electrolyte is carried or sample feeding.Belong to technical field of biochemical industry.
Background technology
The free stream cataphoresis technology is a protein efficient production isolation technics, and its method is to utilize transverse electric field that the protein in the electrolyte solution of continuous flow, virus and cell are carried out compartment analysis.Since the 1970's, FFE has been widely used in the branch analyses of variance such as gene, medicine, protein, cell, organelle and virus.FFE has many good qualities, as the theoretical recovery near 100%, but continuous sample introduction carries out the separation of protein/cell/virus etc.But still there are many problems.
The first, FFE costs an arm and a leg.In FFE, one of gordian technique is the nearly stable conveying of the above damping fluid/electrolytic solution of 96 passages.Traditional method adopt expensive multiple tracks pump realize the conveying of damping fluid (Shao Xiaoxia. Song Jinfang. Wan Qian etc., bioengineering journal, 1997,13,218; Li Qin. Li Li. Wang Zhaojie etc., biological chemistry and biophysics progress, 1998,25,833).Since no any impact mitigation measure in damping fluid is carried, and the FFE separation chamber requires highly stable uniform fluid dynamics environment; Therefore the FFE separation chamber is high to the degree of accuracy and the stability requirement of multiple tracks pump.Because high precision and high stability multiple tracks pump cost an arm and a leg, this causes the FFE cost very high to a great extent.But constant flow pump as adopting, owing to there is labile factor such as tangible pressure pulse, high precision and high stable liquid that therefore general constant flow pump is difficult among the FFE are carried.
The second, the FFE clastotype is single.In existing FFE, the clastotype of electrophoresis is mainly isoelectric focusing electrophoresis (isoelectric focusing, IEF; T.H.Maugh II, Science 1983,222,259), spot speed electrophoresis (isotachophoresis, ITP; W.Thormann, M.A.Firestone, J.E.Sloan, et al.Electrophoresis 1990,11,298) and zone electrophoresis (zone electrophoresis, ZE; S.Hoffstetter-Kuhn, H.Wagner, Electrophoresis 1990,11, and 457).Therefore, in FFE, the clastotype of electrophoresis is more single.In research recently, our first observed arrives the synchronous concentration and separation new model (Qin, W H, Cao, C X, Zhang, W, et al., Electrophoresis, 2005, in press) of MCRB (moving chemical reaction interface)-mediation.This synchronous concentration and separation new model is difficult to finish relevant research and application at traditional FFE.Finish relevant application, need existing FFE device be improved.
Three, FFE seldom is used for sample enrichment.In nearest research, we arrive efficiently concentrating pattern (C.X.Cao, Y.Z.He, M.Li, et al., Anal.Chem.2002,74,4167 of MCRB-mediation at first observed; C.X.Cao, W.Zhang, W.H.Qin, et al., Anal.Chem.2005,77,955).The enrichment new model of this MCRB-mediation is difficult to finish relevant research and application at traditional FFE.Finish relevant application, still need and will existing FFE device be improved.
Summary of the invention
Purpose of the present invention is exactly the problem at above existence, design provides a kind of buffering part flow arrangement that is used for free stream cataphoresis, can eliminate the intrinsic pressure-flow-rate impulse of general infusion pump (as constant flow pump) well, make the pulsed flow of liquid uniformity that becomes, finally in the FFE separation chamber, form stabilized uniform fluid dynamics environment, and can in FFE, finish the synchronous concentration and separation new model of MCRB-mediation and the efficiently concentrating new model of MCRB-mediation.
For achieving the above object, the buffering part flow arrangement that is used for free stream cataphoresis of the present invention's design is by fluid reservoir, air chamber, input pipe, the input pipe interface, efferent duct, the efferent duct interface, the air cushion variable valve, air cushion is regulated syringe, level meter, horizontal adjusting knob and pressure gauge are formed, the input pipe interface is communicated with input pipe, be communicated with infusion pump through input pipe, electrolyte/sample liquids is through infusion pump, input pipe input buffering part flow arrangement inner chamber, many efferent duct interfaces of buffering part flow arrangement bottom are communicated with efferent duct, efferent duct is communicated with the FFE separation chamber, such one or several infusion pumps can be simultaneously carry out liquid to tens even the hundreds of bar conveyance conduit of FFE and carry, behind the buffering part flow arrangement, become the fluid of high stability by the pressure-flow-rate impulse liquid of buffering pneumatic cushion discharge pump conveying, the adjustment that the adjusting by regulating knob and the indication of level meter come the implement device horizontal level realizes the size of input end and the output end pressure of FFE by manometric indication.
Concrete structure of the present invention is: antifluctuator cavity is made up of bottom fluid reservoir and top air chamber, input pipe is communicated with electrolyte/sample solution through infusion pump, the input pipe interface of input pipe through being positioned at the antifluctuator cavity side is communicated with antifluctuator cavity, the efferent duct of antifluctuator cavity through being positioned at cavity bottom is communicated with the FFE separation chamber with the efferent duct interface, air cushion is regulated the air cushion variable valve of syringe through being connected the antifluctuator cavity top and is communicated with antifluctuator cavity, level meter is installed in the top of device, the horizontality of device is indicated by level meter, the bottom of device is provided with two horizontal adjusting knobs, is communicated with a pressure gauge on the top of device.
On the cavity of buffering part flow arrangement, be carved with volume markings.
Buffering part flow arrangement of the present invention can be divided into versions such as vertical type drum type, rectangle curved face type, rectangle plagiohedral according to the difformity of antifluctuator cavity.The antifluctuator cavity of vertical type drum type designs for right cylinder; The antifluctuator cavity bottom of rectangle curved face type is designed for curved, and top is rectangular design; The antifluctuator cavity bottom of rectangle plagiohedral is designed for beveled, and top is rectangular design.Which kind of version no matter, liquid is divided into whole antifluctuator cavity the air chamber and the bottom fluid reservoir on top.
During device work, infusion pump one end connects electrolyte/sample solution, the other end is communicated with antifluctuator cavity through input pipe and input pipe interface, infusion pump input certain amount of fluid is closed the air cushion variable valve to antifluctuator cavity, in antifluctuator cavity, form the fluid reservoir of bottom and the air chamber on top like this, the amount of air cushion indoor gas is regulated syringe by air cushion variable valve and air cushion and can further be adjusted, bottom at the buffering part flow arrangement, design has multiple tracks efferent duct interface, and the efferent duct interface is communicated with the separation chamber of FFE device through efferent duct.Because gas has very strong pressure-flow velocity buffer action in the air chamber, pressure-flow-rate impulse liquid that infusion pump is carried is behind the buffering part flow arrangement and become steadily evenly, buffering part flow arrangement horizontality realizes that by the level meter indication and through the adjustment of horizontal adjusting knob pressure in the air chamber and volume are respectively by barometer and volume markings indication.
The present invention has following advantage and effect:
1) reduces pressure-flow-rate impulse that various infusion pumps cause, effectively, made it to change into continuously fluid (Pulse-Free Flow) uniformly; 2), reduced the dependence of FFE effectively to the multiple tracks pump of high precision high stability; 3), only adopt 1-2 platform discharge pump to the carrying of FFE separation chamber liquid, simplified operation steps; 4), significantly reduce the cost of transfusion system, reduced overall cost.
The present invention has following application:
Electrolyte when 1), conventional FFE separates/or damping fluid/or the stable conveying of sample liquid; 2), utilize the present invention in FFE, to carry out more clastotype, comprise the synchronous concentration and separation pattern of MCRB-mediation; 3), utilize the present invention in FFE, to carry out the sample efficiently concentrating, comprise the example enrichments such as protein of MCRB-mediation.
Description of drawings
Fig. 1 is the structural representation of vertical type drum type buffering part flow arrangement of the present invention.
Among Fig. 1,1 be fluid reservoir, 2 for air chamber, 3 for input pipe, 4 for the input pipe interface, 5 for efferent duct, 6 for the efferent duct interface, 7 for the air cushion variable valve, 8 for air cushion regulate syringe, 9 for level meter, 10 be horizontal adjusting knob, 11 is pressure gauge, and 12 is volume markings.
Fig. 2 is the structural representation of the horizontal curved face type buffering of the present invention part flow arrangement.
Among Fig. 2,1 be fluid reservoir, 2 for air chamber, 3 for input pipe, 4 for the input pipe interface, 5 for efferent duct, 6 for the efferent duct interface, 7 for the air cushion variable valve, 8 for air cushion regulate syringe, 9 for level meter, 10 be horizontal adjusting knob, 11 is pressure gauge, and 12 is volume markings.
Fig. 3 is the structural representation of the horizontal plagiohedral buffering of the present invention part flow arrangement.
Among Fig. 3,1 be fluid reservoir, 2 for air chamber, 3 for input pipe, 4 for the input pipe interface, 5 for efferent duct, 6 for the efferent duct interface, 7 for the air cushion variable valve, 8 for air cushion regulate syringe, 9 for level meter, 10 be horizontal adjusting knob, 11 is pressure gauge, and 12 is volume markings.
Embodiment
Below in conjunction with drawings and Examples technical scheme of the present invention is described further.
Fig. 1 is one embodiment of the present of invention---the structural representation of vertical type drum type buffering part flow arrangement.As shown in Figure 1, buffering part flow arrangement of the present invention is made up of fluid reservoir 1, air chamber 2, input pipe 3, input pipe interface 4, efferent duct 5, efferent duct interface 6, air cushion variable valve 7, air cushion adjusting syringe 8, level meter 9, horizontal adjusting knob 10 and pressure gauge 11.Antifluctuator cavity is made up of fluid reservoir 1 and air chamber 2, is carved with volume markings 12 on the antifluctuator cavity.Input pipe 3 is communicated with electrolyte/sample solution through infusion pump, input pipe 3 is communicated with antifluctuator cavity through input pipe interface 4, antifluctuator cavity connects the FFE separation chamber through efferent duct 5 and efferent duct interface 6, air cushion is regulated syringe 8 and is communicated with antifluctuator cavity through air cushion variable valve 7, level meter 9 is installed in the top of device, the horizontality of device is by level meter 9 indications, and the bottom of device is provided with horizontal adjusting knob 10, is communicated with a pressure gauge 11 on the top of device.
During device work, infusion pump one end connects electrolyte/sample solution, the other end is communicated with antifluctuator cavity through input pipe 3 and input pipe interface 4, infusion pump input certain amount of fluid is closed air cushion variable valve 7 to antifluctuator cavity, form the fluid reservoir 1 of bottom and the air chamber 2 on top like this in antifluctuator cavity, the amount of gas and pressure can further be adjusted by air cushion variable valve 7 and air cushion adjusting syringe 8 in the air chamber 2.In the bottom of buffering part flow arrangement, design has multiple tracks efferent duct interface 6, and efferent duct interface 6 is communicated with the separation chamber of FFE device through efferent duct 5.Because gas has very strong pressure buffer effect in the air chamber 2, pressure-flow-rate impulse liquid that infusion pump is carried is behind the buffering part flow arrangement and become steadily evenly.The buffering part flow arrangement is wanted horizontal positioned, and its horizontality is by level meter 9 indications, and the adjusting of horizontality is realized by the horizontal adjusting knob 10 of bottom.Pressure in the air chamber is by barometer 11 indications, and the volume of fluid reservoir 1 and air chamber 2 is by volume markings 12 indications.
Vertical type drum type buffering part flow arrangement shown in Figure 1 designs for right cylinder, and cylindrical antifluctuator cavity comprises air chamber 2 and fluid reservoir 1, is easy to processing.
Fig. 2 is an another embodiment of the present invention---the structural representation of rectangle curved face type buffering part flow arrangement.Basic structure is similar to Example 1.The antifluctuator cavity bottom is designed for curved, and top is rectangular design.Liquid is divided into whole antifluctuator cavity the air chamber 2 and the bottom fluid reservoir 1 on top.
Fig. 3 is another embodiment of the present invention---the structural representation of rectangle plagiohedral buffering part flow arrangement.Basic structure is also similar to Example 1.The antifluctuator cavity bottom is designed for beveled, and top is rectangular design.Liquid is divided into whole antifluctuator cavity the air chamber 2 and the bottom fluid reservoir 1 on top.
Claims (5)
1, a kind of buffering part flow arrangement that is used for free stream cataphoresis, it is characterized in that antifluctuator cavity is made up of bottom fluid reservoir (1) and top air chamber (2), be carved with volume markings (12) on the antifluctuator cavity, the input pipe interface (4) of input pipe (3) through being positioned at the antifluctuator cavity side that is communicated with electrolyte/sample solution through infusion pump is communicated with antifluctuator cavity, efferent duct interface (6) and the efferent duct (5) of antifluctuator cavity through being positioned at cavity bottom is communicated with the free stream cataphoresis separation chamber, air cushion is regulated the air cushion variable valve (7) of syringe (8) through being connected the antifluctuator cavity top and is communicated with antifluctuator cavity, level meter (9) is installed in the top of device, the bottom of device is provided with horizontal adjusting knob (10), and device top is communicated with pressure gauge (11).
2, according to the buffering part flow arrangement that is used for free stream cataphoresis of claim 1, it is characterized in that described device is the vertical type drum type, antifluctuator cavity designs for right cylinder.
3, according to the buffering part flow arrangement that is used for free stream cataphoresis of claim 1, it is characterized in that described device is the rectangle curved face type, the antifluctuator cavity bottom is designed for curved, and top is rectangular design.
4, according to the buffering part flow arrangement that is used for free stream cataphoresis of claim 1, it is characterized in that described device is the rectangle plagiohedral, the antifluctuator cavity bottom is designed for beveled, and top is rectangular design.
5, according to the buffering part flow arrangement that is used for free stream cataphoresis of claim 1, it is characterized in that the extreme lower position of described efferent duct interface (6) design in fluid reservoir (1), the liquid that infusion pump is transported to the buffering part flow arrangement through many efferent duct interfaces (6) and with interface efferent duct (5) one to one, form nearly tens stabilized uniform liquid transport models to the hundreds of bar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510024412XA CN1333247C (en) | 2005-03-17 | 2005-03-17 | Buffered split flow device in use for electrophoresis of free stream |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510024412XA CN1333247C (en) | 2005-03-17 | 2005-03-17 | Buffered split flow device in use for electrophoresis of free stream |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1696149A CN1696149A (en) | 2005-11-16 |
| CN1333247C true CN1333247C (en) | 2007-08-22 |
Family
ID=35349057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200510024412XA Expired - Fee Related CN1333247C (en) | 2005-03-17 | 2005-03-17 | Buffered split flow device in use for electrophoresis of free stream |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1333247C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103331098B (en) * | 2013-06-28 | 2015-04-01 | 上海交通大学 | Air exhausting device of free-flow electrophoresis separation cavity and implementation method of air exhausting device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1385622A (en) * | 2002-06-21 | 2002-12-18 | 朱家顺 | Liquid pressure stabilizer |
| US20030201181A1 (en) * | 2000-07-21 | 2003-10-30 | Symyx Technologies, Inc. | Hydrodynamic injector |
-
2005
- 2005-03-17 CN CNB200510024412XA patent/CN1333247C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030201181A1 (en) * | 2000-07-21 | 2003-10-30 | Symyx Technologies, Inc. | Hydrodynamic injector |
| CN1385622A (en) * | 2002-06-21 | 2002-12-18 | 朱家顺 | Liquid pressure stabilizer |
Non-Patent Citations (3)
| Title |
|---|
| 连续自由流电泳样机的研制 李钦等，生物化学与生物物理进展，第25卷第4期 1998 * |
| 连续自由流电泳的研制和应用于分离蛋白质和细胞 邵晓霞等，生物工程学报，第13卷第2期 1997 * |
| 连续自由流电泳的研制和应用于分离蛋白质和细胞 邵晓霞等，生物工程学报，第13卷第2期 1997;连续自由流电泳样机的研制 李钦等，生物化学与生物物理进展，第25卷第4期 1998 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1696149A (en) | 2005-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102549408B (en) | processing analysis unit | |
| CN102574124B (en) | Pressurized fluid delivery system with continuously adjustable precision | |
| CN101971004A (en) | Pipette system and method for measuring viscosity | |
| WO2006045229A1 (en) | A micro-volume liquid ejection system | |
| CN105842324B (en) | A kind of integrated microfluidic chip and its working method | |
| US20180163713A1 (en) | Systems, apparatuses, and methods for cell sorting and flow cytometry | |
| US9897531B2 (en) | Flow cytometry sample separation methods and apparatus | |
| CN1333247C (en) | Buffered split flow device in use for electrophoresis of free stream | |
| CN112063512B (en) | Molecular diagnosis card containing whole extraction, amplification and detection process and application process method thereof | |
| GB1136779A (en) | Method and device for metering liquids into capillary tubes | |
| DK1663494T3 (en) | Method and apparatus for handling small volume fluid samples | |
| US10613016B2 (en) | Particle analyzing apparatus using a measured pressure of an inner space of a syringe device and a stored standard pressure to correct a particle analysis value | |
| CN105466992B (en) | A chip electrophoresis separation and plasma mass spectrometry system | |
| CN208043661U (en) | A kind of gas-liquid separation waste discharge apparatus | |
| CN209984986U (en) | Single-double pump full-automatic chromatography system | |
| CN211978786U (en) | A continuous injection filter device for a chromatographic analysis instrument | |
| CN205483707U (en) | Volume adjustable proportioning device | |
| CN223796520U (en) | Automatic quantitative liquid adding device of liquid chromatograph | |
| CN221303323U (en) | Double-path microtube compensation hydride generating device for ICP-OES sample injection | |
| CN207187530U (en) | A kind of high-precision volatile materials air distribution system | |
| CN217042655U (en) | Electronic pipette | |
| CN221899131U (en) | A liquid quantitative metering device for automatic titrator | |
| CN209237984U (en) | Liquid sample adding device | |
| CN202092999U (en) | Chromatograph bottle for continuous flow as well as sectional sampling and sample storage | |
| CN216499457U (en) | High-precision quantitative liquid adding device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070822 Termination date: 20170317 |