CN1313491C - Cat omega interferon and its coding gene and uses - Google Patents

Abstract

The invention discloses a feline omega interferon, its encoding gene and application. The feline omega interferon is a protein having one of the following amino acid residue sequences: 1) SEQ ID No. 1 in the sequence listing; 2) SEQ ID No. 2 in the sequence listing; 3) SEQ ID No. in the sequence listing №: 3; 4) SEQ ID № in the sequence listing: 4; 5) SEQ ID № in the sequence listing: 5; 6) SEQ ID № in the sequence listing: 6; 7) SEQ ID № in the sequence listing: 7 8) SEQ ID No. in the sequence listing: 8; 9) SEQ ID No. in the sequence listing: 9; 10) SEQ ID No. in the sequence listing: 10; 11) SEQ ID No. in the sequence listing: 11; 12 ) SEQ ID No. in the sequence listing: 12; 13) SEQ ID No. in the sequence listing: 13; 14) Substituting the amino acid residue sequence of SEQ ID No.: 1-13 in the sequence listing by one to ten amino acid residues , missing or added proteins that have anti-infective effects on feline leukemia virus, FIV and canine parvovirus.

Description

Feline omega interferon and its coding gene and application

technical field

The invention relates to protein and its encoding gene and application, in particular to feline omega interferon and its encoding gene and its application in preparing anti-infection drugs for feline leukemia virus, FIV and canine parvovirus.

Background technique

Interferon is a type of naturally occurring protein molecule produced and secreted by cells in response to virus infection or various synthetic and biological inductions, with a molecular weight of 15,000-21,000 Daltons. Two broad classes of interferons have been identified: type I and type II. Type I interferon includes four subtypes of IFN-α, IFN-β, IFN-ω and IFN-τ, and type II interferon mainly includes IFN-γ.

In 1992, people such as Nakamura of Japan Toray Co., Ltd. (Toray Industries, Inc.) isolated the feline interferon gene (Molecular cloning of feline interferon cDNA by direct expression.Biosci Biotechnol Biochem.1992,56 (2): 211-4 for the first time ), and in 1993 it was classified as ω-type interferon (Homogenous production of feline interferon in silkworm by replacing single amino acid code in signal peptide region in recombinant baculovirus and characterization of the product.J.Vet.Med.Sci.1993, 55(2):251-8). In 1994, Intercat ^(R) , a recombinant interferon drug based on it, was launched in Japan and used to treat feline calicivirus infection and dog parvovirus infection. Since then, cat IFN-α, IFN-β and IFN-γ have been isolated successively. However, the article published by Ramani Wonderling et al. in 2002 showed that the cat ω-type interferon gene isolated by Nakamura et al. was 94-99% homologous to the cat α-type interferon gene (Cloning, expression, purification, and biological activity of five feline type Iinterferons. Vet Immunol Immunopathol. 2002, 89(1-2): 13-27). And, in 1997, Roberts RM et al defined the difference between IFN-α and IFN-ω (New and atypical families oftype I interferons in mammals: comparative functions, structures, and evolutionary relationships.Prog Nucleic Acid Res Mol Biol.1997, 56 :287-325), there are two main differences: first, IFN-ω has a conserved amino acid sequence consisting of six amino acid residues at the C-terminus, and this sequence is found in IFN-α, IFN-β and IFN-τ Second, the position and number of prolines are highly conserved. IFN-α has conserved prolines at positions 4, 26, 39, 110 and 139 from the N-terminus, while IFN-ω lacks its own prolines. Proline at position 139 of the N-terminus. The feline ω-type interferon gene isolated by Nakamura et al. does not have the conserved amino acid sequence that the C-terminus of IFN-ω should have, and there is a proline at position 142 from the N-terminus. The interferon gene should actually be the cat alpha interferon gene.

Contents of the invention

The purpose of the present invention is to provide a feline omega interferon and its coding gene.

Feline omega interferon provided by the present invention is derived from domestic cat (Felis sivestris), and is a protein having one of the amino acid residue sequences of the following cat omega interferon family:

1) SEQ ID №: 1 in the sequence listing;

2) SEQ ID №: 2 in the sequence listing;

3) SEQ ID №: 3 in the sequence listing;

4) SEQ ID № in the sequence listing: 4;

5) SEQ ID №: 5 in the sequence listing;

6) SEQ ID №: 6 in the sequence listing;

7) SEQ ID №: 7 in the sequence listing;

8) SEQ ID №: 8 in the sequence listing;

9) SEQ ID №: 9 in the sequence listing;

10) SEQ ID №: 10 in the sequence listing;

11) SEQ ID №: 11 in the sequence listing;

12) SEQ ID №: 12 in the sequence listing;

13) SEQ ID №: 13 in the sequence listing;

14) A protein having an anti-infective effect on feline leukemia virus, FIV and canine parvovirus after the amino acid residue sequence of SEQ ID №: 1-13 in the sequence listing undergoes one to ten amino acid residue substitutions, deletions or additions.

Proteins with amino acid residue sequences of SEQ ID №: 1-13 in the sequence listing can be named as FeIFN-ω1 to FeIFN-ω13 respectively, and SEQ ID №: 1-11 in the sequence listing consists of 196 amino acid residues, and SEQ ID №: 12-13 consists of 203 amino acid residues.

The gene encoding the above-mentioned feline omega interferon is selected from one of the nucleotide sequences of the following feline omega interferon family:

1) The DNA sequence of SEQ ID №: 14 in the sequence listing;

2) The DNA sequence of SEQ ID №: 15 in the sequence listing;

3) The DNA sequence of SEQ ID №: 16 in the sequence listing;

4) The DNA sequence of SEQ ID №: 17 in the sequence listing;

5) The DNA sequence of SEQ ID №: 18 in the sequence listing;

6) The DNA sequence of SEQ ID №: 19 in the sequence listing;

7) The DNA sequence of SEQ ID №: 20 in the sequence listing;

8) The DNA sequence of SEQ ID №: 21 in the sequence listing;

9) The DNA sequence of SEQ ID №: 22 in the sequence listing;

10) The DNA sequence of SEQ ID №: 23 in the sequence listing;

11) The DNA sequence of SEQ ID №: 24 in the sequence listing;

12) The DNA sequence of SEQ ID №: 25 in the sequence listing;

13) The DNA sequence of SEQ ID №: 26 in the sequence listing;

14) A nucleotide sequence that can hybridize to the DNA sequence defined by SEQ ID No.: 14-26 in the sequence listing under high stringency conditions;

15) The DNA sequence of the protein of SEQ ID №: 1 in the coding sequence list;

16) The DNA sequence of the protein of SEQ ID №: 2 in the coding sequence list;

17) The DNA sequence of the protein of SEQ ID №: 3 in the coding sequence list;

18) The DNA sequence of the protein SEQ ID №: 4 in the coding sequence list;

19) The DNA sequence of the protein of SEQ ID №: 5 in the coding sequence list;

20) The DNA sequence of SEQ ID №: 6 protein in the coding sequence list;

21) The DNA sequence of the protein of SEQ ID №: 7 in the coding sequence list;

22) DNA sequence of SEQ ID №: 8 protein in the coding sequence list;

23) The DNA sequence of the protein of SEQ ID №: 9 in the coding sequence list;

24) The DNA sequence of the protein of SEQ ID №: 10 in the coding sequence list;

25) The DNA sequence of the protein of SEQ ID №: 11 in the coding sequence list;

26) The DNA sequence of SEQ ID №: 12 protein in the coding sequence list;

27) The DNA sequence of the protein of SEQ ID №: 13 in the coding sequence list;

The high stringency conditions can be 0.1×SSPE (or 0.1×SSC), 0.1% SDS solution, hybridization at 65°C and membrane washing.

The genes with the polynucleotide sequences of SEQ ID №: 14-26 in the sequence listing can be named as FeIFN-ω1 to FeIFN-ω13 respectively. SEQ ID №: 14-24 in the sequence listing consists of 591 bases, and its coding sequence They are the 1st-591st bases from the 5' end, respectively encoding proteins with the amino acid residue sequences of SEQ ID No.: 1-11 in the sequence listing. The SEQ ID № in the sequence listing: 25-26 is composed of 612 bases, and its coding sequence is from the 1st to the 612th base at the 5' end, respectively encoding the amino acid residues in the SEQ ID №: 12-13 in the sequence listing sequence of proteins.

The expression vector containing the gene of the present invention, the transgenic cell line, the host bacterium and the primer pair for amplifying any fragment of the feline omega interferon coding gene all belong to the protection scope of the present invention.

Another object of the present invention is to provide a method for expressing the above-mentioned feline omega interferon.

The method for expressing the above-mentioned feline ω-interferon provided by the present invention is to introduce the recombinant expression vector containing the above-mentioned feline ω-interferon coding gene into host cells to express the feline ω-interferon.

The host is any prokaryotic or eukaryotic cell that can express foreign genes.

The prokaryotic cells can be Escherichia coli, such as E.coli DH5α, E.coli TB1 or E.coli BL21DE3, etc.

The eukaryotic cells can be yeast cells, mammalian cells, plant cells, etc., including yeast SMD1168H, yeast GS115, yeast X-33, yeast KM71H, COS-7, CHO or BHK-21, etc.

The starting vectors for constructing recombinant expression vectors containing feline omega interferon encoding genes can be pLLp-STII (Gene Dynamics Laboratory, USA), pPICZαA (Invitrogen Corporation, USA), pET vector series, pQE vector series, pPIC9K or pPICZα.

Taking pLLp-STII as the starting vector, the constructed recombinant expression vector containing the disintegrin gene is FeIFN-ω/pLLp-STII.

Taking pPICZαA as the starting vector, the constructed recombinant expression vector containing the disintegrin gene is FeIFN-ω/pPICZαA.

When the host is Escherichia coli, IPTG needs to be added to induce expression, and the concentration of the added IPTG is 0.2-1.0 mM, preferably 0.2 mM.

When the host is yeast, methanol needs to be added to induce expression, and the final concentration of added methanol is 0.1-1.0%, preferably 0.5%.

The culture medium and culture conditions for culturing the host cells containing the disintegrin-encoding gene of the present invention can be the culture medium and culture conditions for culturing the starting host.

The invention also provides an anti-infection drug for feline leukemia virus, feline AIDS virus and canine parvovirus.

The active ingredients of the anti-infective medicine provided by the present invention are the above-mentioned feline omega interferon.

When necessary, one or more pharmaceutically acceptable carriers can also be added to the above anti-infective drugs. The carrier includes conventional diluents, excipients, fillers, binders, wetting agents, disintegrants, absorption promoters, surfactants, adsorption carriers and the like in the pharmaceutical field.

The medicine of the present invention can be made into various forms such as injection, tablet, powder, granule, capsule, oral liquid and the like. The above-mentioned medicines in various dosage forms can be prepared according to conventional methods in the field of pharmacy.

The dosage of the above-mentioned drugs is generally 1-2.5 MU/kg body weight, administered continuously or every other day, and the course of treatment is 3-7 days.

The homology of the amino acid residue sequences of the feline interferon provided by the present invention with sheep and human ω-type interferon is 64% and 58%, respectively. Moreover, its amino acid sequence has the six conservative amino acid sequences that IFN-ω C-terminus should have, and there is no proline near the 139th amino acid residue from the N-terminus, which has the characteristics of ω-type interferon. The feline omega interferon and its encoding gene of the present invention are expected to be prepared as clinical anti-infection drugs for feline leukemia virus, FIV and canine parvovirus, and will play an important role in the prevention and treatment of animal diseases.

Description of drawings

Figure 1 is the SDS-PAGE detection result of the Escherichia coli expression product of cat ω-type interferon gene

Figure 2 is the SDS-PAGE detection result of the yeast expression product of cat ω-type interferon gene

Figure 3 is the SDS-PAGE detection result of the purified recombinant feline ω-type interferon

Detailed ways

The methods used in the following examples are conventional methods unless otherwise specified. All primer synthesis and sequencing work were completed by Shanghai Sangong. Furthermore, the experiments showed that the results for the 13 different feline omega interferon subtype genes were consistent in all the experiments below.

Embodiment 1, the cloning of cat omega interferon gene

1. Primer design

According to the conserved amino acid residue sequence of the interferon family, and with reference to the human ω-interferon gene sequence, a pair of primers were designed, and the primer sequences were as follows:

P1: 5'-atggccctcctgctccctct-3'

P2: 5'-tcaagatgassccaggtctccatc-3'

2. Extraction of cat spleen total RNA

Take about 200 mg spleen tissue from cats with acute peritonitis, cut it into pieces quickly, transfer it to a glass grinder, add 2 mL of Trizol reagent (Invitrogen), mix it with the spleen tissue, place it on ice for 15 minutes, and grind it 10-15 times to make the spleen Cells are fully lysed. Then, transfer the Trizol-spleen tissue homogenate to a 1.5mL RNase-free centrifuge tube, add 1/5 homogenate volume of chloroform, mix vigorously for 15 sec, and centrifuge for 15 min. Take the supernatant, add isopropanol equal to the volume of the supernatant, precipitate at room temperature for 10 minutes, and centrifuge for 10 minutes. The supernatant was discarded, the pellet was washed once by centrifugation with 75% ethanol, and the pellet was placed in air to dry. Finally, 30 μl of double distilled water (RNase-free) was added to dissolve the RNA precipitate to obtain the total RNA of cat spleen.

3. RT-PCR amplification of feline omega interferon gene

Using the extracted cat spleen total RNA as a template, under the guidance of primers P1 and P2, the feline omega interferon gene was amplified using the QIAGEN one-step RT-PCR kit (QIAGEN Company) and referring to the kit instructions. Reaction conditions It is: firstly, 50°C for 30min; secondly, 94°C for 10min; again, 94°C for 30sec, 50°C for 30sec, and 72°C for 1min, a total of 35 cycles; finally, 72°C for 5min. The PCR products were stored at 4°C.

4. Sequencing and sequence analysis

The target DNA amplified by PCR with a size of about 600bp was subcloned into the T vector pGEM-T (promega company), and single clones were selected and sequenced after screening. The sequencing results showed that a total of 13 different interferon subtype gene sequences were obtained , respectively having the nucleotide sequence of SEQ ID №: 14-26 in the sequence listing, the SEQ ID №: 14-24 in the sequence listing consists of 591 bases, and its coding sequence is from the 1st to the 5' end 591 bases, respectively encoding proteins with the amino acid residue sequence of SEQ ID №: 1-11 in the sequence listing; SEQ ID №: 25-26 in the sequence listing consists of 612 bases, and its coding sequence is from The 1st to 612th bases at the 'end encode proteins with the amino acid residue sequences of SEQ ID №: 12-13 in the sequence listing, respectively. The amino acid residue sequence encoded by the isolated gene was compared with the amino acid residue sequence of sheep and human ω-type interferon, and the homology with sheep ω-type interferon was 64%, and that of human ω-type interferon The homology is 58%. The amino acid sequence homology of the interferon isolated by Nakamura et al. is 49% with sheep ω-type interferon, and 53% with the amino acid sequence homology of human ω-type interferon. Moreover, the amino acid sequence encoded by the isolated interferon gene has the six conserved amino acid sequences that should be present at the C-terminus of IFN-ω, and there is no proline near the 139th amino acid residue from the N-terminus, which has the characteristics of ω-type interferon .

Embodiment 2, the expression of feline ω-type interferon gene and the purification of its expression product

1. Expression and fermentation production of feline ω-type interferon gene in Escherichia coli

1. Construction of Escherichia coli expression vector FeIFN-ω/pLLp-STII containing feline ω-type interferon gene

The plasmids containing 13 different gene sequences of feline ω-type interferon subtypes were subcloned into the vector pLLp-STII after double digestion with restriction endonucleases BamHI and HindIII, and the constructed prokaryotic The expression plasmids are collectively called FeIFN-ω/pLLp-STII, and transformed into Escherichia coli DH5α. Positive recombinant bacteria were obtained after identification by PCR and enzyme digestion, the engineered bacteria were amplified and cultured, and the inducer IPTG with a final concentration of 0.2mM was added to induce at 30°C for 3 hours. After the culture, the expression product was detected by SDS-PAGE electrophoresis, and the detection result is shown in Figure 1 (lane M is the low molecular weight protein marker, and lane 1 is the expression product), a protein band appears at about 19kD, which is consistent with the expected result It was consistent, indicating that the feline ω-type interferon gene was correctly expressed in Escherichia coli.

2. Fermentation production of recombinant feline omega interferon

Pick the single colonies of positive clones obtained in step 1, inoculate them in 100 ml of LB liquid medium containing 100 mg/ml ampicillin, and culture them in shake flasks at 37°C for 12 hours as the primary seed solution; The primary seed liquid was transferred to a fermenter containing 6 liters of LB liquid medium, and cultured under the same conditions as step 1 until the _OD600 value was 1.0.

Cold shock treatment of fermentation broth: after the fermentation, centrifuge to collect the bacteria, suspend the bacteria with a solution containing 30mM Tris-HCl, 20% sucrose by mass percentage, 1mM EDTA pH8.0 (80mL per gram of bacteria) Body, ice bath, shake gently for 10 minutes. Then, centrifuge at 8000g at 4°C, remove the supernatant, suspend the pellet with 5mM MgSO ₄ , ice-bath, and shake gently for 10 minutes. Centrifuge at 12000g for 15 minutes, and take the supernatant, which is the fermentation broth after cold shock treatment.

2. Expression and fermentation production of feline ω-type interferon gene in yeast

1. Construction of yeast expression vector FeIFN-ω/pPICZαA containing feline ω-type interferon gene

Respectively, plasmids containing 13 different gene sequences of feline ω-type interferon subtypes were digested with restriction endonucleases XhoI and XbaI and then subcloned into the vector pPICZαA which was double-digested with the same enzymes, and the constructed yeast was secreted and expressed The plasmids are collectively referred to as FeIFN-ω/pPICZαA, and the SMD1168H yeast strain was electrotransformed, and the transformant was spread on YPDS containing zeocin (Invitrogen Company) 100 μg/mL (containing 1% yeast extract, 2% tryptone for yeast culture, 2% Glucose, 1M sorbitol, 2% agar) plate, cultivated at 30°C for 2-3 days until a single colony grows, and positive recombinant bacteria are obtained after PCR identification. Inoculate positive recombinant bacteria into BMGY (containing 1% yeast extract, 2% tryptone for yeast culture, 100mM potassium phosphate (pH 6.0), 1.34% YNB, 4× ^10-5 % biotin, 1% glycerol) In culture medium, cultivate at 30°C for 18h-20h, when the _OD600 value is 2-6, centrifuge at 1500g for 5min to collect the bacteria, and then replace it with BMMY medium (containing 1% yeast extract, 2% tryptone for yeast culture , 100mM potassium phosphate (pH 6.0), 1.34% YNB, 4×10 ^-5 % biotin, 0.5% methanol), continue to cultivate under the same conditions to make its OD ₆₀₀ reach 1, and then add the final concentration of 0.5% every 24 hours The expression of methanol was induced, samples were collected every 24 hours, and induced for 48 hours at 30°C. After the culture, the expression product was detected by SDS-PAGE electrophoresis, and the detection result is shown in Figure 2 (lane M is the low molecular weight protein marker, and lane 1 is the expression product), a protein band appears at about 19kD, which is consistent with the expected result It was consistent, indicating that the feline ω-type interferon gene was correctly expressed in yeast.

2. Fermentation production of recombinant feline omega interferon

The fermenter is Biostat B. of B.Braun Company, with a capacity of 5 liters. The specific fermentation process is as follows: pick the single colonies of positive clones obtained in step 1, and inoculate them in 20 mL of YPD medium containing 0.1 μg/mL zeocin (containing 1% yeast extract, 2% tryptone for yeast culture, 2% glucose) at 30°C and 250 rpm for 30 hours, then inserted into 200 mL of zeocin-free YPD medium, and cultured at 30°C and 250 rpm for 24 hours. After the cultivation, the bacteria were collected by centrifugation, and the bacteria were inoculated into 2 liters of BMGY medium (containing 1% yeast extract, 2% tryptone for yeast culture, 100 mM potassium phosphate (pH 6.0), 1.34% YNB, 4× 10 ^-5 % biotin, 1% glycerol), at 30°C, dissolved oxygen above 30%, pH 5.5, after culturing for 15 hours, add 50% glycerol, flow rate 20mL glycerol/L bacterial solution/hour, when the cells When the wet weight reaches 240g/L, glycerol supplementation is stopped. When the dissolved oxygen rises sharply, the pH value rises, and the glycerol in the medium is exhausted, start to induce expression by adding 24% methanol dropwise. The flow rate is 3.6mL methanol/L bacterial solution/hr, and the Within hours, the concentration of methanol was increased to 70%, and the expression was induced for 5 days, so that the cat omega interferon gene was expressed.

3. Purification of Recombinant Feline Omega Interferon

Take the fermented liquid from step 1 and step 2, centrifuge the fermented liquid in step 1 to get the supernatant after cold shock treatment, and get the supernatant by centrifuging 10000g of the fermented liquid in step 2, centrifuge for 10min to precipitate the bacteria, take the supernatant, adjust the pH value to 4.5 with acetic acid, and perform cation exchange Chromatographic column (HiTrap SP FF, Amersham Biosciences), collect the target elution peak, and then pass through an anion exchange chromatography column (HiTrap Q FF, Amersham Biosciences), collect the target elution peak, pass Sephacryl molecular sieve chromatography column (Amersham Biosciences) , collect the target elution peak, filter and sterilize to obtain purified recombinant feline omega interferon, and carry out SDS-PAGE electrophoresis detection on the purified protein, and the detection results are shown in Figure 3 (swimming lane M is the low molecular weight protein Marker, and swimming lane 1 is Purified product), a protein band appeared at about 19kD, consistent with the expected results, indicating that the feline ω-type interferon base with higher purity was obtained.

Embodiment 3, cytopathic inhibition method identifies the biological activity of recombinant cat omega interferon

Feline embryonic fibroblasts and vesicular stomatitis virus (VSV) were used to determine the antiviral potency of recombinant feline omega interferon. The specific method was: take 15-day-pregnant cat embryos, remove the head, limbs and viscera, and place them in a sterile state. After washing three times with PBS, cut into pieces. After blowing into a single cell, put it on a 96-well plate, culture it until all adherent growth, remove the culture medium, divide it into two groups, and add 4-fold (4 ⁰ -4 ¹¹ U/mL) diluted Example 1 to each well of one group 2 obtained Escherichia coli expressed and purified recombinant feline omega interferon, another group added 4-fold (4 ⁰ -4 ¹¹ U/mL) diluted Pichia pastoris obtained in Example 2 to each well and purified The recombinant feline omega interferon was challenged with 100 TCID ₅₀ doses of virus, the control well was added with virus-free culture medium, and a negative control was set up at the same time (only the recombinant feline omega interferon diluted by doubling ratio was added, and no virus was added), Positive control (no interferon, only virus) and blank control (no interferon, no virus), the results were judged when more than 50% of the lesions appeared in the control wells under an inverted microscope. Observed after 24 hours, 100% serious lesions appeared in the positive control wells, and the cell wells were added with 4 ^10, 4 ⁹ and 4 ⁸ U/mL diluted recombinant feline omega interferon expressed in Escherichia coli and purified respectively 70%, 40%, and 0% of the ^lesions appeared, and the cells were in good condition in ^the cell wells that were added with a concentration of interferon higher than ^{4-8 .} The cell wells added after U/mL doubling dilution had 80%, 30%, and 0% lesions respectively, and the cell wells added with a concentration higher than ^4-9 interferon were in good condition. Negative control wells contained normal cells. The above results indicated that the biological activity of the recombinant feline omega interferon expressed in Escherichia coli was 4 ⁸ U/mL, and that of the recombinant feline omega interferon expressed in Pichia pastoris was 4 ⁹ U/mL.

Embodiment 4, safety experiment

1. Take 200 cats from 9 months to 2 years old (half male and half male), each intravenously inject 10 ⁷ U/kg recombinant feline omega interferon, as a result, 76 cats have slight lethargy, and the symptoms disappear after 5-6 hours . Twenty-five cats had a slight increase in body temperature, but it was within the normal range. Electrolyte indicators in body fluids were normal.

2. Take 200 Beagle dogs (half male and half female) aged one to seven years old, and inject 10 ⁷ U/kg of recombinant feline omega interferon intravenously into each. As a result, 5 dogs have slight lethargy, and symptoms appear after 5-6 hours disappear. Electrolyte indicators in body fluids were normal.

Embodiment 5, the clinical trial effect of recombinant feline omega interferon

1. Take 30 cats infected with leukemia virus and divide them into a treatment group and a control group, 15 in each group. The treatment group was subcutaneously injected with 1MU/kg of recombinant feline omega interferon every day, and the control group was injected with placebo for three consecutive days. The results showed that the recombinant feline omega interferon could effectively treat feline leukemia virus infection, and the condition of the diseased cats improved obviously, as the cell number increased to normal or close to normal, and fever symptoms disappeared.

2. Get 30 Beagle dogs (half male and half female) from one year old to 7 years old, and divide them into administration group and control group, with 15 dogs in every group. All dogs were vaccinated against type II canine parvovirus (CPV) until symptoms of parvovirus infection appeared. Then, the administration group was intravenously injected with 2.5MU/kg of recombinant feline omega interferon every day, and the control group was only injected with placebo for three consecutive days. The results showed that the recombinant feline omega interferon could effectively treat canine parvovirus infection, and the condition of the affected dogs improved significantly. The specific manifestations were that the number of white blood cells rose to normal or close to normal, and the symptoms of fever, vomiting and diarrhea disappeared.

Sequence Listing

<160>26

<210>1

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>1

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Trp Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Pro Ala Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Ala Ser Ser

195

<210>2

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>2

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Leu Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Pro Ala Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Ala Ser Ser

195

<210>3

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>3

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Val Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Leu Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Ser Ala Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Gly Ser Ser

195

<210>4

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>4

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Trp Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Pro Val Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Gly Ser Ser

195

<210>5

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>5

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Leu Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Pro Ala Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Gly Ser Ser

195

<210>6

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>6

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Gln Gln Leu Glu Ala Leu Asp Ala Cys Leu Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Leu Gly Met His Gly Pro Ala Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Ala Ser Ser

195

<210>7

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>7

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Trp Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Pro Val Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Ala Ser Ser

195

<210>8

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>8

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Gln Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Val Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Leu Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Pro Ala Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Ala Ser Ser

195

<210>9

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>9

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Trp Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Leu Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Pro Ala Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Glu Asp

180 185 190

Leu Gly Ser Ser

195

<210>10

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>10

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Leu Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Pro Ala Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Gly Leu Ser

195

<210>11

<211>196

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>11

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Thr Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Gly Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Leu Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Pro Gly Met His Gly Pro Asp Leu Ala Ile

130 135 140

Lys Arg Tyr Phe Gln Asp Ile Arg Val Tyr Leu Glu Asp Glu Gly Tyr

145 150 155 160

Ser Asp Cys Ala Trp Glu Ile Val Arg Leu Glu Ile Met Arg Ala Leu

165 170 175

Ser Ser Ser Ala Thr Leu Gln Asp Ser Leu Ala Ile Lys Asp Gly Asp

180 185 190

Leu Gly Ser Ser

195

<210>12

<211>203

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>12

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Ala Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

115 120 125

Gly Glu Gly Glu Arg Ala Thr Gly Glu Gly Glu Arg Ala Pro Gly Met

130 135 140

His Gly Pro Val Leu Ala Ile Lys Arg Tyr Phe Gln Asp Ile Arg Val

145 150 155 160

Tyr Leu Glu Asp Glu Gly Tyr Ser Asp Cys Ala Trp Glu Ile Val Arg

165 170 175

Leu Glu Ile Met Arg Ala Leu Ser Ser Ser Ala Thr Leu Gln Asp Ser

180 185 190

Leu Ala Ile Lys Asp Gly Asp Leu Gly Ser Ser

195 200

<210>13

<211>203

<212>PRT

<213> Domestic cat (Felis sivestris)

<400>13

Met Ala Leu Leu Leu Pro Leu Leu Thr Ala Leu Ala Leu Leu Thr Cys

1 5 10 15

Arg Pro Gly Gly Ser Leu Gly Cys Ala Leu Pro Gly Ser His Ala Gln

20 25 30

Val Ser Arg Asp Asn Leu Val Leu Leu Gly Gln Met Arg Arg Leu Ser

35 40 45

Pro Phe Leu Cys Leu Arg Ala Arg Lys Asp Phe Arg Phe Pro Arg Glu

50 55 60

Met Leu Glu Gly Gly Gln Leu Arg Glu Ala Gln Ala Ala Ala Thr Val

65 70 75 80

Leu Arg Glu Leu Leu Gln Gln Thr Phe Asn Leu Leu His Thr Glu Arg

85 90 95

Ser Ser Ala Ala Trp Ser Pro Ala Pro Leu His Gly Leu Arg Ser Gly

100 105 110

Leu His Arg Gln Leu Glu Ala Leu Asp Ala Cys Leu Leu Gln Ala Thr

115 120 125

Gly Glu Gly Glu Arg Ala Thr Gly Glu Gly Glu Arg Ala Pro Gly Met

130 135 140

His Gly Pro Val Leu Ala Ile Lys Arg Tyr Phe Gln Asp Ile Arg Val

145 150 155 160

Tyr Leu Glu Asp Glu Gly Tyr Ser Asp Cys Ala Trp Glu Ile Val Arg

165 170 175

Leu Glu Ile Met Arg Ala Leu Ser Ser Ser Ala Thr Leu Gln Asp Ser

180 185 190

Leu Ala Ile Lys Asp Gly Asp Leu Ala Ser Ser

195 200

<210>14

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>14

atggccctcc tgctccctct gctgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct ggctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggccct 420

gccctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcaggctg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat ggagacctgg cgtcatcttg a 591

<210>15

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>15

atggccctcc tgctccctct actgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggccct 420

gccctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcaggctg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat ggagacctgg cgtcatcttg a 591

<210>16

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>16

atggccctcc tgctccctct gctgaccgcc ctggtgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggctct 420

gccctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcagactg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat ggagacctgg gctcatcttg a 591

<210>17

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>17

atggccctcc tgctccctct gctgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct ggctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggccct 420

gtcctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcaggctg gaaatcatga gagccttgtc ctcctcggcg 540

accttacaag acagcttggc catcaaggat ggagacctgg gctcatcttg a 591

<210>18

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>18

atggccctcc tgctccctct gctgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcttgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggccct 420

gccctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcaggctg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat ggagacctgg gctcatcttg a 591

<210>19

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>19

atggccctcc tgctccctct gctgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accagcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccctggggat gcacggccct 420

gccctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcagactg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat ggagacctgg cgtcatcttg a 591

<210>20

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>20

atggccctcc tgctccctct gctgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct ggctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggccct 420

gtcctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcaggctg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat ggagacctgg cgtcatcttg a 591

<210>21

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>21

atggccctcc tgctccctct actgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcaggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg tgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggccct 420

gccctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcagactg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat ggagacctgg cgtcatcttg a 591

<210>22

<211>591

<212> DNA

<213> Domestic cat (Felis sivestris)

<400>22

atggccctcc tgctccctct gctgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctctgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggccct 420

gccctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcaggctg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat gaagacctgg gctcatcttg a 591

<210>23

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>23

atggccctcc tgctccctct gctgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggccct 420

gccctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcaggctg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat ggagacctgg gcttatcttg a 591

<210>24

<211>591

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>24

atggccctcc tgctccctct gctgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc cgggagccac acgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggggccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccccggggat gcacggccct 420

gacctggcca tcaagaggta cttccaggac atccgcgtct acctggagga cgagggatac 480

agtgactgcg cctgggaaat tgtcaggctg gaaatcatga gagccttgtc ctcctcggcg 540

accttgcaag acagcttggc catcaaggat ggagacctgg gctcatcttg a 591

<210>25

<211>612

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>25

atggccctcc tgctccctct actgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccgccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccacgggcga gggagagcgc 420

gccccgggga tgcacggccc tgtcctggcc atcaagaggt acttccagga catccgcgtc 480

tacctggagg acgagggata cagtgactgc gcctgggaaa ttgtcaggct ggaaatcatg 540

agagccttgt cctcctcggc gaccttgcaa gacagcttgg ccatcaagga tggagacctg 600

ggctcatctt ga 612

<210>26

<211>612

<212>DNA

<213> Domestic cat (Felis sivestris)

<400>26

atggccctcc tgctccctct gctgaccgcc ctggcgctgc tcacctgccg ccctggaggc 60

tctctgggct gtgccctgcc tgggagccac gcgcaggtta gcagggacaa cttggtgctc 120

ctgggccaga tgcggagact gtcccctttc ttgtgcctgc gggccagaaa agacttccgc 180

ttcccccggg agatgctgga gggcggccag ctccgggagg cccaggccgc cgccaccgtc 240

ctgcgggagc tgctccagca gaccttcaac ctgttgcaca cggagcgctc ctcggcggcc 300

tggagccccg cgccgctgca cggactccgc tctggcctcc accggcagct ggaagccctg 360

gacgcctgct tgctgcaggc cacgggcgag ggagagcgcg ccacgggcga gggagagcgc 420

gccccgggga tgcacggccc tgtcctggcc atcaagaggt acttccagga catccgcgtc 480

tacctggagg acgagggata cagtgactgc gcctgggaaa ttgtcaggct ggaaatcatg 540

agagccttgt cctcctcggc gaccttgcaa gacagcttgg ccatcaagga tggagacctg 600

gcgtcatctt ga 612

Claims (12)

1. Feline omega interferon is a protein having one of the amino acid residue sequences of the following cat omega interferon family: 1) SEQ ID №: 1 in the sequence listing; 2) SEQ ID №: 2 in the sequence listing; 3) SEQ ID №: 3 in the sequence listing; 4) SEQ ID № in the sequence listing: 4; 5) SEQ ID №: 5 in the sequence listing; 6) SEQ ID №: 6 in the sequence listing; 7) SEQ ID №: 7 in the sequence listing; 8) SEQ ID №: 8 in the sequence listing; 9) SEQ ID №: 9 in the sequence listing; 10) SEQ ID №: 10 in the sequence listing; 11) SEQ ID №: 11 in the sequence listing; 12) SEQ ID №: 12 in the sequence listing; 13) SEQ ID №: 13 in the sequence listing; 14) A protein having an anti-infective effect on feline leukemia virus, FIV and canine parvovirus after the amino acid residue sequence of SEQ ID №: 1-13 in the sequence listing undergoes one to ten amino acid residue substitutions, deletions or additions. 2. The gene encoding the feline omega interferon of claim 1, selected from one of the nucleotide sequences of the following feline omega interferon family: 1) The DNA sequence of SEQ ID №: 14 in the sequence listing; 2) The DNA sequence of SEQ ID №: 15 in the sequence listing; 3) The DNA sequence of SEQ ID №: 16 in the sequence listing; 4) The DNA sequence of SEQ ID №: 17 in the sequence listing; 5) The DNA sequence of SEQ ID №: 18 in the sequence listing; 6) The DNA sequence of SEQ ID №: 19 in the sequence listing; 7) The DNA sequence of SEQ ID №: 20 in the sequence listing; 8) The DNA sequence of SEQ ID №: 21 in the sequence listing; 9) The DNA sequence of SEQ ID №: 22 in the sequence listing; 10) The DNA sequence of SEQ ID №: 23 in the sequence listing; 11) The DNA sequence of SEQ ID №: 24 in the sequence listing; 12) The DNA sequence of SEQ ID №: 25 in the sequence listing; 13) The DNA sequence of SEQ ID №: 26 in the sequence listing; 17) A nucleotide sequence that can hybridize to the DNA sequence defined by SEQ ID No.: 14-26 in the sequence listing under high stringency conditions; 18) The DNA sequence of the protein of SEQ ID №: 1 in the coding sequence list; 19) The DNA sequence of SEQ ID №: 2 protein in the coding sequence list; 20) The DNA sequence of the protein of SEQ ID №: 3 in the coding sequence list; 21) DNA sequence of SEQ ID №: 4 protein in the coding sequence list; 22) The DNA sequence of the protein of SEQ ID №: 5 in the coding sequence list; 23) The DNA sequence of the protein of SEQ ID №: 6 in the coding sequence list; 24) The DNA sequence of the protein of SEQ ID №: 7 in the coding sequence list; 25) The DNA sequence of the protein of SEQ ID №: 8 in the coding sequence list; 26) The DNA sequence of the protein of SEQ ID №: 9 in the coding sequence list; 27) The DNA sequence of the protein of SEQ ID №: 10 in the coding sequence list; 28) The DNA sequence of the protein of SEQ ID №: 11 in the coding sequence list; 29) The DNA sequence of SEQ ID №: 12 protein in the coding sequence list; 30) The DNA sequence encoding the protein of SEQ ID №: 13 in the sequence listing. 3. The expression vector containing the feline omega interferon coding gene of claim 2. 4. A transgenic cell line containing the feline omega interferon coding gene of claim 2. 5. A host bacterium containing the expression vector of claim 3. 6. A method for expressing the feline omega interferon as claimed in claim 1, comprising introducing the recombinant expression vector containing the feline omega interferon coding gene as claimed in claim 2 into host cells to express feline omega interferon. 7. The method for feline omega interferon according to claim 6, characterized in that: the starting vectors for constructing the recombinant expression vector containing the feline omega interferon coding gene of claim 2 are pLLp-STII, pPICZαA, pET vector series, pQE vector series, pPIC9K or pPICZα. 8. The method for feline ω-interferon according to claim 7, characterized in that: the recombinant expression vector is FeIFN-ω/pLLp-STII. 9. The method for feline ω-interferon according to claim 7, characterized in that: the recombinant expression vector is FeIFN-ω/pPICZαA. 10. The method according to claim 6 or 7 or 8 or 9, characterized in that: the host is E.coli DH5α, E.coli TB1, E.coli DE3, yeast SMD1168H, yeast GS115, yeast X -33 or yeast KM71H. 11. The method according to claim 6 or 7 or 8 or 9, characterized in that: when the host is Escherichia coli, IPTG needs to be added to induce expression, and the concentration of IPTG added is 0.2-1 mM; the host For yeast, methanol needs to be added to induce expression, and the final concentration of added methanol is 0.1-1.0%. 12. The use of the feline omega interferon as claimed in claim 1 in the preparation of medicaments against feline leukemia virus, FIV or canine parvovirus infection.

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