CN1306020C - Polyporus frondosus oral liquor and its prodn. method - Google Patents
Polyporus frondosus oral liquor and its prodn. method Download PDFInfo
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- CN1306020C CN1306020C CNB200410083062XA CN200410083062A CN1306020C CN 1306020 C CN1306020 C CN 1306020C CN B200410083062X A CNB200410083062X A CN B200410083062XA CN 200410083062 A CN200410083062 A CN 200410083062A CN 1306020 C CN1306020 C CN 1306020C
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention discloses a polyporus frondosus oral liquor and a producing method thereof, which comprises the following steps: step 1, fermentation liquor is obtained; step 2, the fermentation liquor is heated; step 3, the fermentation liquor is prepared, 0.3 to 1.5 percent of animal protein hydrolyzed liquid, 1.0 to 5.0 percent of sweetening agent, 0.05 to 0.8 percent of souring agent and 0.05 to 0.2 percent of stabilizer are added to 93.5 to 98.45 percent of the fermentation liquor containing mycelium by weight percent, and the pH is regulated from 4.0 to 7.0; step 4, the fermentation liquor is treated in a high-pressure and homogeneous way, is firstly treated by a colloid mill, and then is treated by a high-pressure homogenizing machine; step 5, the fermentation liquor is sterilized to fill by cans. The present invention fully uses the deep fermentation liquor of the polyporus frondosus fungi to obtain the polyporus frondosus oral liquor by heat, preparation and high-pressure homogeneous treatment, completely retains various nutrient components in the fermentation liquor of the polyporus frondosus fungi, realizes high-efficiency dissolution and complete utilization for mycelium function factors, and reaches clean fermentation production.
Description
Technical field
The invention belongs to the edible fungus manufacture field, particularly relating to Grifola frondosa fungi submerged fermentation liquid is a kind of Grifola frondosa oral liquid of main material production and production method thereof.
Technical background
Grifola frondosa (Polyporus frondosus) is under the jurisdiction of Mycophyta, Basidiomycotina, Aphyllophorales, polyporaceae, Polyporus.Grifola frondosa is famous edible, medicinal fungi, and is nutritious, tasty, is the macro fungi of " but edible, can mend medicine, the whole body is precious ".Grifola frondosa is flat, flavor is sweet, can be used for treating diseases such as dysuria, oedema, beriberi, hepatic ascites, diabetes, hypertension and obesity.Grifolan has tangible antitumous effect.Recently, FDA has confirmed that the Grifola frondosa extract has the unusual effect of treatment advanced breast cancer and prostate cancer, and allows the effective inhibitor of Grifola frondosa extract as cancer, directly carries out the II clinical trial phase.Maitake mushroom mycelia has broad application prospects in medicine, food; And the fermentation clear liquid that liquid submerged fermentation method production Grifola frondosa obtains also is rich in functional factors such as grifolan, has bigger value of exploiting and utilizing.
Correlative study both at home and abroad mainly concentrates on aspects such as the extraction of Grifola frondosa functional factor and pharmacology, drug effect, nourishing function.And in liquid submerged fermentation method production Grifola frondosa process, be purpose to obtain maitake mushroom mycelia how, do not contain mycelial fermentation clear liquid to go out of use, make that nutritive ingredient can not make full use of in the fermentation clear liquid, and environment is polluted.
At present, the Grifola frondosa oral liquid product on the domestic market has a kind of Grifola frondosa oral liquid product of being processed into Grifola frondosa, Ganoderma sporophore etc. that nine Pharma Inc.s in Beijing produce.
Summary of the invention:
The present invention overcomes the wasting of resources that exists in the present Grifola frondosa fermentative production and absorbs inefficient defective, the invention provides a kind of Grifola frondosa oral liquid product; Another technical problem that the present invention solves provides a kind of production method of Grifola frondosa oral liquid product.
Technical scheme of the present invention is summarized as follows:
A kind of Grifola frondosa oral liquid consists of by weight percentage: contain mycelial fermented liquid 93.5%～98.45%, animal protein factor digest 0.3～1.5%, sweeting agent 1.0～5.0%, acidic flavoring agent 0.05～0.8%, stablizer 0.05～0.2% is through the acquisition of (1) fermented liquid; (2) fermented liquid heating; (3) allotment of fermented liquid; (4) the high-pressure homogeneous processing of fermented liquid; (5) sterilization filling makes, and Grifola frondosa oral liquid pH is 4.0～7.0.
Sweeting agent can be selected sucrose or honey.
Acidic flavoring agent can select citric acid or aromatic vinegar.
Stablizer can select Xylo-Mucine or xanthan gum or gelatin.
A kind of production method of Grifola frondosa oral liquid comprises the steps:
(1) acquisition of fermented liquid: adopt slant strains to spread cultivation step by step and obtain Grifola frondosa fungi submerged fermentation liquid;
(2) fermented liquid heating: Grifola frondosa fungi submerged fermentation liquid is heated to 60～90 ℃, kept 10～30 minutes, reach go out enzyme and pre-heat effect;
(3) allotment of fermented liquid: will contain mycelial fermented liquid 93.5%～98.45% by weight percentage, the animal protein factor digest of adding 0.3～1.5%, 1.0～5.0% sweeting agent, 0.05～0.8% acidic flavoring agent, 0.05～0.2% stablizer is regulated PH 4.0～7.0;
(4) the high-pressure homogeneous processing of fermented liquid: the fermented liquid after will allocating flows to colloidal mill, and the gap of adjusting colloidal mill stator and rotor is 10～50 microns, and mycelium in the fermented liquid is carried out fragmentation, and the colloidal mill flow is 1～5 ton/hour; Handle through high pressure homogenizer, adjusting high pressure homogenizer pressure is 20～60Mpa again, and high-pressure homogeneous flow is 0.5～3 ton/hour, and sediments microscope inspection mycelium sporoderm-broken rate is 100%;
(5) sterilization filling: the fermented liquid after will handling was sterilized 3～30 seconds at 95 ℃～135 ℃, and sterilization finishes and carries out can, promptly obtains a kind of Grifola frondosa oral liquid.
(6) packing of product: products obtained therefrom is packed, be the Grifola frondosa oral liquid product after the packing.
The preparation method of animal protein factor digest is in the present embodiment: get a certain amount of chicken (beef or pork) and rub through mincer, chicken after the rubbing and water are with 1: 2～5 mixed and through milling treatment of colloid, the gap of adjusting colloidal mill stator and rotor is 50～100 microns, and the colloidal mill flow control is 0.1～0.5 ton/hour; With chicken (beef or pork) the adjustment temperature after milling treatment of colloid is 40～60 ℃, PH is 5.0～8.0, the papoid or the flavor protease of adding 0.01～0.1% carry out enzymolysis, and enzymolysis obtains chicken (beef or pork) protein hydrolyte after 4～6 hours.
Because the present invention all utilizes Grifola frondosa fungi submerged fermentation liquid (containing mycelium) directly to obtain Grifola frondosa oral liquid through heating, allotment and high-pressure homogeneous processing, therefore the various nutritive ingredients in the Grifola frondosa fungal fermented filtrate (containing mycelium) have been kept fully, high-pressure homogeneous treatment process has been realized the efficient stripping of mycelium functional factor, and human body is improved greatly to the specific absorption of various nutritional factor in the Grifola frondosa oral liquid product.Overcome present most enterprise simultaneously only for obtaining the weak point that maitake mushroom mycelia will not contain mycelial fermentation clear liquid abandoned well discharging, realized full use nutritive ingredient in the fermentation clear liquid; Realized the cleaning production of the no emissions of Grifola frondosa fungal fermented filtrate production process, product has been filled up the market vacancy.Owing to process homogenization treatment in this product, be rich in the Grifola frondosa oral liquid and be easy to the functional factors such as grifolan that absorption of human body is utilized simultaneously; Product has good physiotherapy function, can reach anti-curing cancers and antitumous effect.Grifola frondosa oral liquid is that a kind of ready-to-eat with nutrition, nourishing function is the drinkable condiment for cooking that also can be used as simultaneously.
Embodiment
The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Embodiment 1
Produce 100 kilograms of Grifola frondosa oral liquids, the liquid submerged fermentation method of learning from else's experience obtains after producing and handling by heating process contains 95.5 kilograms of mycelial fermented liquids, add 1 kilogram of animal protein factor digest, 1 kilogram of sucrose, 2 kilograms of honey, 0.4 kilogram of citric acid, 0.1 kilogram of allotment of carrying out fermented liquid of Xylo-Mucine; Make Grifola frondosa oral liquid through the high-pressure homogeneous treatment process of fermented liquid, sterilization filling again, control Grifola frondosa oral liquid pH is 7.0.
In the present embodiment, the preparation method of animal protein factor digest is: get a certain amount of chicken and rub through mincer, chicken after the rubbing and water are with 1: 3 mixed and through milling treatment of colloid, and the gap of adjusting colloidal mill stator and rotor is 70 microns, and the colloidal mill flow control is 0.3 ton/hour; With the adjustment of the beef after milling treatment of colloid temperature is 55 ℃, and PH is 5.5, and the papoid of adding 0.06% carries out enzymolysis, and enzymolysis obtained beaf protein hydrolyzed solution after 5 hours.
Embodiment 2
Produce 100 kilograms of Grifola frondosa oral liquids, what obtain after acquisition, the fermented liquid heating back technology of fermented liquid of learning from else's experience contains 95 kilograms of mycelial fermented liquids, add 1.5 kilograms of animal protein factor digests (preparation is with embodiment 1), 2 kilograms of sucrose, 0.95 kilogram of honey, 0.05 kilogram of aromatic vinegar, 0.05 kilogram of allotment of carrying out fermented liquid of xanthan gum; Make Grifola frondosa oral liquid through the high-pressure homogeneous treatment process of fermented liquid, sterilization filling again, control Grifola frondosa oral liquid pH is 6.0.
Embodiment 3
Produce 100 kilograms of Grifola frondosa oral liquids, what obtain after acquisition, the fermented liquid heating back technology of fermented liquid of learning from else's experience contains 93.5 kilograms of mycelial fermented liquids, add 0.5 kilogram of animal protein factor digest (preparation is with embodiment 1), 5 kilograms of sucrose, 0.8 kilogram of aromatic vinegar, 0.2 kilogram of allotment of carrying out fermented liquid of gelatin; Make Grifola frondosa oral liquid through the high-pressure homogeneous treatment process of fermented liquid, sterilization filling again, control Grifola frondosa oral liquid pH is 5.0.
Embodiment 4
Produce 100 kilograms of Grifola frondosa oral liquids, what obtain after acquisition, the fermented liquid heating back technology of fermented liquid of learning from else's experience contains 98.45 kilograms of mycelial fermented liquids, add 0.3 kilogram of animal protein factor digest (preparation is with embodiment 1), 1 kilogram of sucrose, 0.05 kilogram of citric acid, 0.2 kilogram of allotment of carrying out fermented liquid of Xylo-Mucine; Make Grifola frondosa oral liquid through the high-pressure homogeneous treatment process of fermented liquid, sterilization filling again, control Grifola frondosa oral liquid pH is 4.0.
Embodiment 5
A kind of production method of Grifola frondosa oral liquid comprises the steps:
1. the acquisition of fermented liquid: adopt slant strains to spread cultivation step by step and obtain Grifola frondosa fungi submerged fermentation liquid;
(1) first order seed is cultivated: the Grifola frondosa slant strains is inserted in 500 ml shake flasks 100 milliliters of substratum loading amounts, 180 rev/mins of rotary shaking tables, 24 ℃ of culture temperature, incubation time 72 hours;
(2) secondary seed is cultivated: first order seed is inserted 500 milliliters of secondary seeds according to 10% inoculum size shake in the bottle, culture condition is identical with first order seed;
(3) three grades of seed culture: secondary seed is inserted 5000 milliliters of three grades of seeds with 10% inoculum size shake in the bottle 1000 milliliters of substratum loading amounts, 100 rev/mins of rotary shaking tables, 24 ℃ of culture temperature, incubation time 50 hours;
(4) first class seed pot is cultivated: it is the first class seed pot of 150L that three grades of seeds are inserted cubic capacity with 10% inoculum size, fermention medium loading amount 100L, 24 ℃ of culture temperature, 150 rev/mins of stirring velocitys, ventilation (V/V) 1: 0.5, tank pressure 0.05Mpa, incubation time 48 hours;
(5) the secondary seed jar is cultivated: it is 1.5 tons of secondary seed jars that the first class seed pot bacterial classification is inserted cubic capacity with 10% inoculum size, 1 ton of fermention medium loading amount, and culture condition is identical with first class seed pot;
(6) fermentor cultivation: it is 13 tons fermentor tank that the bacterial classification in the secondary seed jar is inserted cubic capacity with 10% inoculum size, 10 tons of fermention medium loading amounts, 24 ℃ of culture temperature, 100 rev/mins of stirring velocitys, ventilation (V/V) 1: 0.4, tank pressure 0.05Mpa, incubation time 72 hours.
2. fermented liquid heating: Grifola frondosa fungi submerged fermentation liquid is heated to 70 ℃, kept 20 minutes, reach go out enzyme and pre-heat effect.
3. the allotment of fermented liquid: the ratio in embodiment 1 is allocated.
4. the high-pressure homogeneous processing of fermented liquid: the fermented liquid after will allocating flows to colloidal mill, and the gap of adjusting colloidal mill stator and rotor is 10 microns, and mycelium in the fermented liquid is carried out fragmentation, and the colloidal mill flow is 1 ton/hour; Handle through high pressure homogenizer, adjusting high pressure homogenizer pressure is 40Mpa again, and high-pressure homogeneous flow is 1 ton/hour, and sediments microscope inspection mycelium sporoderm-broken rate is 100%.
5. it is canned to sterilize: the fermented liquid after will handling was sterilized 10 seconds at 120 ℃, and sterilization finishes and carries out can, promptly obtains-kind of Grifola frondosa oral liquid.
6. the packing of product: products obtained therefrom is packed, be the Grifola frondosa oral liquid product after the packing.
Embodiment 6
A kind of production method of Grifola frondosa oral liquid comprises the steps:
1. the acquisition of fermented liquid: adopt slant strains to spread cultivation step by step and obtain Grifola frondosa fungi submerged fermentation liquid; (with embodiment 5)
2. fermented liquid heating: Grifola frondosa fungi submerged fermentation liquid is heated to 60 ℃, kept 30 minutes, reach go out enzyme and pre-heat effect;
3. the allotment of fermented liquid: the ratio in embodiment 2 is allocated;
4. the high-pressure homogeneous processing of fermented liquid: the fermented liquid after will allocating flows to colloidal mill, and the gap of adjusting colloidal mill stator and rotor is 30 microns, and mycelium in the fermented liquid is carried out fragmentation, and the colloidal mill flow is 3 tons/hour; Handle through high pressure homogenizer, adjusting high pressure homogenizer pressure is 60Mpa again, and high-pressure homogeneous flow is 3 tons/hour, and sediments microscope inspection mycelium sporoderm-broken rate is 100%;
5. it is canned to sterilize: the fermented liquid after will handling was sterilized 30 seconds at 95 ℃, and sterilization finishes and carries out can, promptly obtains a kind of Grifola frondosa oral liquid;
6. the packing of product: products obtained therefrom is packed, be the Grifola frondosa oral liquid product after the packing.
Embodiment 7
A kind of production method of Grifola frondosa oral liquid comprises the steps:
1. the acquisition of fermented liquid: adopt slant strains to spread cultivation step by step and obtain Grifola frondosa fungi submerged fermentation liquid; (with embodiment 5)
2. fermented liquid heating: Grifola frondosa fungi submerged fermentation liquid is heated to 90 ℃, kept 10 minutes, reach go out enzyme and pre-heat effect;
3. the allotment of fermented liquid: the ratio in embodiment 3 is allocated;
4. the high-pressure homogeneous processing of fermented liquid: the fermented liquid after will allocating flows to colloidal mill, and the gap of adjusting colloidal mill stator and rotor is 50 microns, and mycelium in the fermented liquid is carried out fragmentation, and the colloidal mill flow is 5 tons/hour; Handle through high pressure homogenizer, adjusting high pressure homogenizer pressure is 20Mpa again, and high-pressure homogeneous flow is 0.5 ton/hour, and sediments microscope inspection mycelium sporoderm-broken rate is 100%;
5. it is canned to sterilize: the fermented liquid after will handling was sterilized 3 seconds at 135 ℃, and sterilization finishes and carries out can, promptly obtains a kind of Grifola frondosa oral liquid;
6. the packing of product: products obtained therefrom is packed, be the Grifola frondosa oral liquid product after the packing.
The present invention adopts the phenolsulfuric acid method to measure grifolan content: get product 10ml, adding distil water 100ml is in the backflow flask, refluxing extraction is 2 hours in boiling water, and is with extracting solution at 5000rpm centrifugal 10 minutes, once centrifugal more under the same conditions after residual quarrel cleaned.Collect supernatant liquor in beaker, being concentrated in vacuo to volume is 10～20ml.Add 3 times of 95% ethanol after the cooling, refrigerator is placed and is spent the night.At 3000rpm centrifugal 10 minutes then, the collecting precipitation thing washed throw out in the 1000ml volumetric flask adding distil water constant volume with distilled water.Get 2ml and put in the 10ml measuring bottle, the accurate 5% phenol test solution 1ml mixing that adds adds sulfuric acid 5.0ml rapidly, jolting 5 minutes is put and is placed in the boiling water bath after 15 minutes, and cooling is 30 minutes in cold water, add water to scale and shake up, measure the OD value, do blank simultaneously at 412nm.Calculate corresponding polysaccharide content according to measurement result.
Slant medium of the present invention consists of: potato (liquor) 20%, glucose 2%, potassium primary phosphate 0.1%, sal epsom 0.05%, agar 2.0%.
The Grifola frondosa oral liquid product standard sees Table 1.
Table 1 Grifola frondosa oral liquid product standard
| Sense organ requires (enterprise's mark) | Project | Requirement |
| Color and luster | Light brown | |
| Form | Evenly liquid. | |
| Impurity | Inclusion-free. | |
| Physics and chemistry requires (enterprise's mark) | Water content (%) | ≤ 90 |
| Grifolan mg/100ml | 〉=150 | |
| Protein g/100ml | 〉=2 | |
| Microbiological indicator (GB 16740-1997) | Coliform (MPN/100ml) | ≤ 40 |
| Total plate count (cfu/ml) | ≤ 1000 | |
| Pathogenic bacterium | Must not detect | |
| Mould (cfu/ml) | ≤ 10 | |
| Yeast (cfu/ml) | ≤ 10 | |
| Plumbous (in pb) | ≤ 0.5mg/kg | |
| Arsenic (in As) | ≤ 0.3mg/kg |
First order seed, secondary seed, three grades of seeds, first class seed pot, secondary seed jar and fermentative production substratum compositions are among the present invention: W-Gum 5%, yeast extract paste 0.5%, sucrose 1%, dipotassium hydrogen phosphate 0.2%, sal epsom 0.1%, etiocobalamin Pm, PH6.0.
The used Grifola frondosa bacterial classification of the present invention is purchased in Chinese industrial microbial strains preservation center, bacterium CICC 14080.
Claims (5)
1. a Grifola frondosa oral liquid makes via following method: the acquisition of (1) fermented liquid: adopt slant strains to spread cultivation step by step and obtain Grifola frondosa fungi submerged fermentation liquid; (2) fermented liquid heating: Grifola frondosa fungi submerged fermentation liquid is heated to 60～90 ℃, kept 10～30 minutes; (3) allotment of fermented liquid: will contain mycelial fermented liquid 93.5%～98.45% by weight percentage, the animal protein factor digest of adding 0.3～1.5%, 1.0～5.0% sweeting agent, 0.05～0.8% acidic flavoring agent, 0.05～0.2% stablizer is regulated PH 4.0～7.0; (4) the high-pressure homogeneous processing of fermented liquid: the fermented liquid after will allocating flows to colloidal mill, and the gap of adjusting colloidal mill stator and rotor is 10～50 microns, and the colloidal mill flow is 1～5 ton/hour; Handle through high pressure homogenizer, adjusting high pressure homogenizer pressure is 20～60Mpa again, and high-pressure homogeneous flow is 0.5～3 ton/hour, and sediments microscope inspection mycelium sporoderm-broken rate is 100%; (5) sterilization filling: the fermented liquid after will handling was sterilized 3～30 seconds at 95 ℃～135 ℃, and sterilization finishes and carries out can, promptly obtains a kind of Grifola frondosa oral liquid.
2. a kind of Grifola frondosa oral liquid according to claim 1 is characterized in that described sweeting agent is sucrose or honey.
3. a kind of Grifola frondosa oral liquid according to claim 1 is characterized in that described acidic flavoring agent is citric acid or aromatic vinegar.
4. a kind of Grifola frondosa oral liquid according to claim 1 is characterized in that described stablizer is Xylo-Mucine or xanthan gum or gelatin.
5. the production method of the described Grifola frondosa oral liquid of claim 1, step is: the acquisition of (1) fermented liquid; (2) fermented liquid heating; (3) allotment of fermented liquid; (4) the high-pressure homogeneous processing of fermented liquid; (5) sterilization filling makes, and Grifola frondosa oral liquid pH is 4.0～7.0.
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| CN102742897A (en) * | 2011-04-22 | 2012-10-24 | 天津科技大学 | Grifola frondosus suspension beverage and its preparation method |
| CN102742896A (en) * | 2011-04-22 | 2012-10-24 | 天津科技大学 | Grifola frondosus oral liquid and its production method |
| CN102771856A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Grifola frondosa health drink prepared by liquid-submerged fermentation and preparation method of Grifola frondosa health drink |
| CN102805335B (en) * | 2012-07-20 | 2015-07-29 | 北京厚文知识产权顾问有限公司 | A kind of preparation method of edible fungus health product |
| CN103549432A (en) * | 2013-11-15 | 2014-02-05 | 哈尔滨艾克尔食品科技有限公司 | Preparation method of Yun Xun oral liquid |
| CN104068379B (en) * | 2014-06-26 | 2016-03-30 | 天津和治友德制药有限公司 | A kind of health care oral liquid |
| CN106333183A (en) * | 2015-07-06 | 2017-01-18 | 许昌世纪香生物科技有限公司 | Beverage for treating hepatic ascites |
| CN105434344B (en) * | 2016-01-08 | 2019-04-05 | 福建农林大学 | A kind of anti-enterovirns type 71 oral solution of grifolan and preparation method thereof |
| CN109673981A (en) * | 2018-12-01 | 2019-04-26 | 哈尔滨伟平科技开发有限公司 | A kind of HuGan oral liquid and preparation method thereof |
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| CN1339581A (en) * | 2001-09-29 | 2002-03-13 | 李宝健 | Large-scale deep liquid fermentation process for gray tree flower |
| CN1398979A (en) * | 2002-08-16 | 2003-02-26 | 维京仲华（上海）生物医药科技有限公司 | Fermentation process of ash tree flower and production process of its polyglycopeptide |
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| CN1339581A (en) * | 2001-09-29 | 2002-03-13 | 李宝健 | Large-scale deep liquid fermentation process for gray tree flower |
| CN1398979A (en) * | 2002-08-16 | 2003-02-26 | 维京仲华（上海）生物医药科技有限公司 | Fermentation process of ash tree flower and production process of its polyglycopeptide |
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