CN1348007A - Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol - Google Patents
Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol Download PDFInfo
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- CN1348007A CN1348007A CN 01117282 CN01117282A CN1348007A CN 1348007 A CN1348007 A CN 1348007A CN 01117282 CN01117282 CN 01117282 CN 01117282 A CN01117282 A CN 01117282A CN 1348007 A CN1348007 A CN 1348007A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 31
- 230000004151 fermentation Effects 0.000 title claims abstract description 30
- 230000000813 microbial effect Effects 0.000 title claims abstract description 8
- YPFDHNVEDLHUCE-UHFFFAOYSA-N propane-1,3-diol Chemical compound OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 title abstract 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 44
- 239000007788 liquid Substances 0.000 claims abstract description 8
- UXFQFBNBSPQBJW-UHFFFAOYSA-N 2-amino-2-methylpropane-1,3-diol Chemical compound OCC(N)(C)CO UXFQFBNBSPQBJW-UHFFFAOYSA-N 0.000 claims description 30
- 235000011187 glycerol Nutrition 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- 239000011573 trace mineral Substances 0.000 claims description 6
- 235000013619 trace mineral Nutrition 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 3
- 229910052750 molybdenum Inorganic materials 0.000 claims description 3
- 239000011733 molybdenum Substances 0.000 claims description 3
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 2
- 235000005979 Citrus limon Nutrition 0.000 claims description 2
- 241000193403 Clostridium Species 0.000 claims description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- 241000588748 Klebsiella Species 0.000 claims description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052796 boron Inorganic materials 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 239000010941 cobalt Substances 0.000 claims description 2
- 229910017052 cobalt Inorganic materials 0.000 claims description 2
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 239000011777 magnesium Substances 0.000 claims description 2
- 229910052749 magnesium Inorganic materials 0.000 claims description 2
- -1 molybdenum Chemical compound 0.000 claims description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims 1
- 244000248349 Citrus limon Species 0.000 claims 1
- 230000036983 biotransformation Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 229910017053 inorganic salt Inorganic materials 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 9
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 abstract 4
- 229920000166 polytrimethylene carbonate Polymers 0.000 abstract 4
- 238000010564 aerobic fermentation Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 7
- 239000002609 medium Substances 0.000 description 5
- 238000011218 seed culture Methods 0.000 description 5
- 230000009466 transformation Effects 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- AKXKFZDCRYJKTF-UHFFFAOYSA-N 3-Hydroxypropionaldehyde Chemical compound OCCC=O AKXKFZDCRYJKTF-UHFFFAOYSA-N 0.000 description 2
- HGINCPLSRVDWNT-UHFFFAOYSA-N Acrolein Chemical compound C=CC=O HGINCPLSRVDWNT-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229910021591 Copper(I) chloride Inorganic materials 0.000 description 2
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical class Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- KKEYFWRCBNTPAC-UHFFFAOYSA-N Terephthalic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-N 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 description 2
- 238000007323 disproportionation reaction Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 229920002215 polytrimethylene terephthalate Polymers 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 241000370738 Chlorion Species 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 108010025885 Glycerol dehydratase Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
本发明属于生物工程技术领域,特别涉及到一种微生物微氧发酵法生产1,3－丙二醇的技术。本发明的特征在于所使用的微生物细胞不仅在厌氧条件下,而且在微氧条件下都能将甘油转化为1,3－丙二醇。其优点是发酵工艺简单经济,既简化了操作条件,降低了生产成本,又缩短了发酵时间,提高了生产效率,而且微氧条件下发酵液中1,3－丙二醇的浓度与厌氧发酵相当或略高一些,为微生物发酵法生产1,3－丙二醇的工业化提供了简单经济的发酵工艺。The invention belongs to the technical field of bioengineering, and in particular relates to a technology for producing 1,3-propanediol by micro-aerobic fermentation. The present invention is characterized in that the used microbial cells can convert glycerol into 1,3-propanediol not only under anaerobic conditions but also under microaerobic conditions. The advantage is that the fermentation process is simple and economical, which not only simplifies the operating conditions, reduces the production cost, but also shortens the fermentation time and improves the production efficiency, and the concentration of 1,3-propanediol in the fermentation liquid under micro-aerobic conditions is equivalent to that of anaerobic fermentation. Or slightly higher, providing a simple and economical fermentation process for the industrialization of microbial fermentation to produce 1,3-propanediol.
Description
The invention belongs to technical field of bioengineering, specially refer to a kind of microorganism and produce 1 at little oxygen condition bottom fermentation, the method for ammediol.
As everyone knows, 1, ammediol is a kind of important chemical material, with ethylene glycol, 1,2-propylene glycol and 1, the 4-butyleneglycol has same purposes, but it and terephthalic acid synthetic new polyester material polytrimethylene terephthalate (PTT) have many excellent characteristic, good continuous printing and dyeing that present as need not to add any speciality chemical in the tint permanence of recovery of elasticity, uvioresistant, ozone and the oxynitride of nylon sample, low static, low water absorption, the panchromatic scope and biodegradable etc.These have all shown 1, the application prospect that ammediol is fine, but expensive price has hindered its application.
Present 1, the production method of ammediol mainly is a chemical synthesis, is raw material with ethene as Shell Co. Ltd, under high temperature (280 ℃), become oxyethane as catalyst oxidation with silver, hydrogenation and carbon monoxide change into the 3-hydroxy propanal then, are hydrogenated to product 1 at last, ammediol; Degussa company then is raw material with the propylene, is propenal with molybdenum as catalyst oxidation under 350 ℃, 0.2Mpa, and rehydration is the 3-hydroxy propanal, is hydrogenated to 1 then, ammediol (USP 5,008,473).These two kinds of methods all need be carried out under high temperature and valuable catalyst action, and product removes 1, and ammediol also has 1 outward, 2-propylene glycol and the close by product of character such as dimer, tripolymer thereof, and it is difficult to cause product separation to be purified, and production cost is corresponding higher.
The microbial method glycerine converting is 1, and the research of ammediol starts from 1881, but just causes people's attention gradually up to the eighties in 20th century.Compare with chemical synthesis, it has mild condition, easy and simple to handle, characteristics such as by product is few, non-environmental-pollution.Present 1, the microorganisms producing method of ammediol can be divided into two classes: the one, and be that substrate produces 1 with genetic engineering bacterium with glucose, (PCT/US 96/06705 for ammediol; USP 5,599, and 689; WO 96/35796; WO 9821340; WO9821339), the 2nd, be 1 with intestinal bacteria with the glycerine disproportionation, ammediol (USP 5,254, and 467; EP 0,373,230 A1).The former transformation efficiency and production concentration are all lower, and the latter is then higher relatively.Other microbe fermentation method of publishing in the method for above-mentioned patent report and the document all is anaerobically fermenting without exception, this is because be 1 in transformation of glycerol, there are some key enzymes in the process of ammediol, as glycerol dehydrogenase, Protosol kinases glycerol dehydratase, 1, ammediol desaturase etc., generally these enzymes under anaerobic play a role, and oxygen will cause indivedual key enzyme inactivations, as the irreversible inactivation of glycerol dehydrogenase, thereby can not generate 1, ammediol.
The objective of the invention is to utilize amphimicrobes such as klebsiella, lemon bacterium and clostridium, being implemented under little oxygen condition transformation of glycerol is 1, the zymotechnique of ammediol.The invention solves under little oxygen condition and can not produce 1, the technical barrier of ammediol, the amphimicrobe after long-term domestication can not only be 1 with transformation of glycerol under anaerobic, ammediol, and under little oxygen condition, also can produce 1, ammediol.
The step that realizes the inventive method is as follows:
1) medium preparation: must possess the required nutritive ingredient of microorganism growth in the substratum, as carbon sources such as glycerine or glucose, nitrogenous source such as yeast extract or yeast powder, negatively charged ion such as positively charged ions such as sodium, potassium, ammonia, magnesium, calcium and phosphate radical, sulfate radical, chlorion, and trace elements such as zinc, iron, manganese, copper, cobalt, boron and molybdenum.Substratum must be sterilized down at 121 ℃ and can be used in 15～20 minutes.
2) seed culture: carry out in shaking bottle, shaking speed is 100～300 rev/mins, and temperature is 27～40 ℃, and incubation time is 9～24 hours.
3) fermentation culture: can carry out in fermentor tank, the fermentor tank inoculum size is 5%～20%, and rotating speed of agitator is 100～400 rev/mins, and temperature is 27～40 ℃, and pH is controlled at 6.0～8.0.Can in fermentor tank, lead to nitrogen or air in the fermenting process, also can stuffiness, air flow is 0～2vvm.Fermentation mode can be that batch fermentation, batch formula stream add fermentation or continuously ferments, intermittently or batch formula stream time of adding fermentation be 10～100 hours, in the fermented liquid 1, the concentration of ammediol can reach 5～70g/L; Earlier carry out batch fermentation when continuously fermenting, when treating that organism dry weight concentration reaches 0.2～2.0g/L in the fermented liquid, again with 0.1～0.5h with 1%～6% glycerin medium
-1Thinning ratio stream add fermention medium and carry out the perseverance cultivation.
The invention provides a kind of microbial fermentation and produce 1, the method for ammediol, used microorganism cells can both be 1 with transformation of glycerol under anaerobism and little oxygen condition, ammediol, and productive rate is suitable.The benefit of this method is to have simplified Production by Microorganism Fermentation 1, and the technology of ammediol has reduced production cost, has shortened fermentation time, has improved production efficiency, is Production by Microorganism Fermentation 1, and the industrialization of ammediol is laid a good foundation.
Below be described in detail most preferred embodiment of the present invention:
Used bacterial classification is Cray Bai Shi bacillus (Klebsiella pneumoniae) in the embodiment of the invention, available from Chinese common micro-organisms DSMZ (CGMCC), and culture presevation number: 1.1736.
Substratum divides two kinds of seed culture medium and fermention mediums:
1) seed culture medium: (1000ml):
Glycerine: 20g K
2HPO
43H
2O:4.56g
KH
2PO
4：1.3g (NH
4)
2SO
4：2.0g
MgSO
47H
2O:2g yeast powder: 1g
CaCO
3: 2g trace element A:2ml
0.5%FeSO
4Solution: 1ml 2%CaCl
2Solution: 1ml
Trace element A forms (1000ml):
Saturated hydrochloric acid: 0.9ml ZnCl
2: 70mg
MnCl
2·4H
2O：100mg H
3BO
3：60mg
CoCl
2·6H
2O：200mg NiCl
2·6H
2O：25mg
NaMoO
42H
2O:35mg CuCl
22H
2O:20mg2) fermention medium is formed (1000ml):
(NH
4)SO
4：6.61g KH
2PO
4：1.36g
MgCl
26H
2O:0.26 citric acid: 0.42g
Yeast powder: 1g trace element B:5ml
Trace element B forms (1000ml):
Saturated hydrochloric acid: 10ml ZnCl
26H
2O:0.68g
FeCl
3·6H
2O：5.4g MnCl
2·4H
2O：0.17g
CoCl
2·6H
2O：0.47g H
3BO
3：0.06g
NaMoO
4·2H
2O：0.005g?CuCl
2·2H
2O：0.47g
Seed and fermention medium all will be regulated pH before sterilization be 7.0
Fermenting process branch seed culture and two steps of fermentation culture: seed culture is carried out in the triangular flask of a 500ml, and liquid amount is 200ml, and shaking speed is 200 commentaries on classics/min, and culture temperature is 37 ℃, and incubation time is 12hr; Fermentation culture is carried out in automatic fermenter, and working volume is 5L, and actual liquid amount is 3L, and temperature and invariablenes turning speed are 37 ℃ and 300 commentaries on classics/min, regulates pH7.0 with 2mol/L potassium hydroxide.
Concrete fermenting experiment result is as follows: ferment in fermentor tank with containing 2% glycerin medium, in the anaerobic condition bottom fermentation liquid 1, the concentration of ammediol is 7.8g/L, be 8.2g/L under the stuffiness condition, is 9.4g/L when leading to the air of 1.2L/min; The glycerine disproportionation is 1, and the molar yield of ammediol is respectively 47.0%, 49.6% and 56.9%, and the fermentation ends time was respectively 18,15 and 11 hours.When the glycerine starting point concentration is 4%, batch fermentation obtains under anaerobism, stuffiness and ventilation (air of the 1.2L/min) condition 1, ammediol concentration is respectively 15.7,16.8,15.1g/L, molar yield is respectively 43.7%, 50.8%, 45.7%, fermentation time was respectively 32,20 and 17 hours.
Claims (1)
1. a microbial micro-aerobe fermentation produces 1, the method of ammediol, in temperature, under pH and the mixing speed constant condition, carry out intermittence, batch formula stream adds or continuously ferments the glycerine bio-transformation is 1, ammediol, used microbial strains is a Klebsiella, amphimicrobes such as lemon bacterium and Clostridium, in the substratum except essential carbon source, outside nitrogenous source and some inorganic salt, also should contain zinc, iron, magnesium, copper, cobalt, boron, trace elements such as molybdenum, the fermentation inoculum size is 5%～20%, temperature is controlled at 27 ℃～40 ℃, pH is 6.0～8.0, intermittently or batch formula stream to add fermentation time be 10～100 hours, in the fermented liquid 1, the ultimate density of ammediol can reach 5～70g/L.This inventive method is characterised in that:
A) fermenting process can carry out under little oxygen condition, and air feeding amount is 0～2vvm;
B) during batch fermentation in the substratum mass percent concentration of glycerine be 2%～20%, the glycerol concentration of adding when batch formula stream adds fermentation is 10%～100%, glycerol concentration in the substratum that continuously ferments
Be 2%～20%;
C) continuously ferment be with 1%～6% initial glycerol concentration carry out thalline give be cultured to cell concentration reach contain 0.2～2.0g stem cell in every liter of fermented liquid after, again with 0.1～0.5h
-1The thinning ratio Continuous Flow add the perseverance culturing process of fermention medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01117282 CN1204260C (en) | 2001-04-27 | 2001-04-27 | Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01117282 CN1204260C (en) | 2001-04-27 | 2001-04-27 | Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1348007A true CN1348007A (en) | 2002-05-08 |
| CN1204260C CN1204260C (en) | 2005-06-01 |
Family
ID=4662685
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01117282 Expired - Fee Related CN1204260C (en) | 2001-04-27 | 2001-04-27 | Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1204260C (en) |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006128381A1 (en) * | 2005-06-03 | 2006-12-07 | Tsinghua University | Method for preparing 1,3-propanediol by using glycerine as the by-product of the biological diesel oil |
| WO2006133637A1 (en) * | 2005-06-17 | 2006-12-21 | Tsinghua University | Method for preparing 1,3-propanediol and 2,3-tuanediol by the coarse starch material |
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2001
- 2001-04-27 CN CN 01117282 patent/CN1204260C/en not_active Expired - Fee Related
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| CN100430111C (en) * | 2003-05-06 | 2008-11-05 | 泰特&莱尔组分美国公司 | Process for producing 1,3-propanediol |
| CN1320104C (en) * | 2003-09-15 | 2007-06-06 | 天津医科大学 | Bacterial strain for preparing high efficiency capsule glycoprotein immunomodulator, screening and application thereof |
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| WO2006128381A1 (en) * | 2005-06-03 | 2006-12-07 | Tsinghua University | Method for preparing 1,3-propanediol by using glycerine as the by-product of the biological diesel oil |
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| CN101230362B (en) * | 2008-02-27 | 2012-07-04 | 东南大学 | Method for effectively producing 1,3-propanediol by modifying permeability of cell membrane |
| CN101254969B (en) * | 2008-03-31 | 2010-04-07 | 清华大学 | A method for preparing microbial flocculant by using the by-product cells of the fermentation industry |
| WO2009140929A1 (en) * | 2008-05-04 | 2009-11-26 | 清华大学 | A method for co-production of 1, 3-propanediol, 2,3-butanediol and polyhydroxypropionic acid by fermentation of constructed genetic engineering bacteria |
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| CN102140480B (en) * | 2010-06-09 | 2013-07-17 | 河南天冠企业集团有限公司 | Method for producing 1,3-propylene glycol by fermentation |
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| CN102311979B (en) * | 2010-07-07 | 2013-07-24 | 中国石油化工股份有限公司 | Method for promoting to biosynthesize 1,3-propylene glycol by adding reducing sugar |
| CN106957877A (en) * | 2016-01-11 | 2017-07-18 | 中国石油化工股份有限公司 | A kind of utilization microorganism conversion produces the method and device of 1,3- propane diols |
| CN106957877B (en) * | 2016-01-11 | 2020-06-05 | 中国石油化工股份有限公司 | Method and device for producing 1, 3-propylene glycol by microbial conversion |
| CN112111534A (en) * | 2020-09-09 | 2020-12-22 | 大连理工大学 | A method for preparing 1,3-propanediol-coupled phage produced by microbial fermentation |
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