CN1234068A - Method of enhancing antimicrobial activity of aqueous antimicrobial liquid cleaning formulations - Google Patents

Abstract

A method for enhancing the antimicrobial activity of an aqueous antimicrobial liquid cleansing formulation comprising the steps of combining a polymeric deposition aid comprising a mixture of a liquid, hydroxyl-terminated urethane polymer in polyethylene glycol with a phenol derivative antimicrobial agent and a surfactant, the resulting liquid cleansing formulation having an antimicrobial activity at least 10% greater than that of the same formulation without the polymeric deposition aid.

Description

Method of enhancing antimicrobial activity of aqueous antimicrobial liquid cleaning formulations

field of invention

The present invention relates to a method of enhancing the antimicrobial efficacy of antibacterial liquid formulations for personal cleansing.

Background of the invention

Certain types of surfactants are known to have a tendency to inhibit the antimicrobial activity of certain phenol-derived antimicrobials. Examples of phenol derivative antibacterial agents include, for example, triclosan, phenoxyethanol, chloroxylenol, o-phenylphenol, o-phenylphenate, and the like. Nonionic and cationic surfactants are thought to have specific negative effects on antimicrobial activity. Their activity is said to be highly dependent on the pH of the system. Such surfactants can even completely eliminate antimicrobial activity.

Based on this, many aqueous antibacterial liquid preparations for personal use contain other types of surfactants and compounding additives to minimize interference with the antibacterial activity of phenol derivative antibacterial agents. However, these surfactants and compounding additives can still reduce the activity of some phenol derivative antibacterial agents.

Therefore, there is a need for a simple and economical method for enhancing the activity of aqueous antibacterial liquid cleaning formulations containing phenol derivative antibacterial agents. This need can be broadened to a method of enhancing the activity of aqueous antimicrobial liquid cleansing formulations containing anionic and cationic surfactants. This need also extends to formulations containing primarily nonionic and amphoteric surfactants and/or surfactant systems.

Summary of the invention

The above-mentioned problems are solved by a method for enhancing the antibacterial activity of an aqueous antibacterial liquid cleaning formulation containing a phenol derivative antibacterial agent and a surfactant or a surfactant system provided by the present invention. The method comprises the steps of mixing a polymeric deposition aid consisting of a liquid, hydroxyl-terminated mixture of urethane polymers in polyethylene glycol with a phenol derivative antimicrobial and a surfactant or surfactant system, The antimicrobial activity of the liquid cleaning formulation thus obtained is at least 10% higher than the same formulation without the polymeric deposition aid. For example, the methods of the present invention can increase the antimicrobial activity of an aqueous antimicrobial liquid cleansing formulation by at least 20% over the same formulation without the polymeric deposition aid. The formulations may also include one or more conventional formulation ingredients including, but not limited to, carriers, preservatives, humectants, emollients, mixtures thereof, and the like. In general, greater antimicrobial activity is expressed as a percentage reduction in microbial colony counts as determined by, for example, the R.O.D.A.C. (Replicate Organism Detection And Counting) plate test technique.

According to the present invention, the polymeric deposition aid is a hydroxyl-terminated urethane compound of the formula:

structural formula

wherein R is selected from alkylene or alkenylene groups containing 1 to about 20 carbon atoms, cycloalkylene or cycloalkenylene groups containing about 5 to about 10 carbon atoms, and containing about 6 to about 10 carbon atoms Atomic, unsubstituted or substituted by one or more lower alkyl, lower alkoxy, nitro or amino or halogen atoms, mononuclear or fused-ring arylene; R' are the same or different alkylene or alkenylene; wherein m is an integer selected from the group consisting of (O-R') moieties having a molecular weight of from about 40 to about 6000; and

wherein n and n' are the same or different integers from 0 to about 30 inclusive, which are related to m such that the hydroxyl-terminated urethane compound has a molecular weight of up to about 200,000.

Ideally, m is 8 and the values of n and n' are mainly 1-4. The values of m, n and n' are related so that the molecular weight of the hydroxyl terminated urethane is about 1800 is also desirable.

In an embodiment of the invention, the polymeric deposition aid may be poly(oxy-1,2-ethanediyl), alpha-hydro-omega-hydroxy-, and 1,1'-methylene-di- (4, Poly(oxy-1,2-ethanediyl)α-hydro-ω-hydroxy-,polymer with 1,1'-methylene-bis-(4,isocyanatocyclohexane) ).

Phenol derivative antimicrobial agents may be selected from 2,4,4'-trichloro-2'-hydroxydiphenyl ether (also known as triclosan), phenoxyethanol, chloroxylenol, o-phenylphenol and o-phenylphenol Phenylphenoxide. Ideally, the phenol derivative antimicrobial agent is 2,4,4'-trichloro-2'-hydroxydiphenyl ether.

According to the invention, at least one surfactant or surfactant system is mixed with other ingredients. The surfactant or surfactants may consist of one or more anionic, cationic, nonionic and/or amphoteric surfactants. At least one conventional formulation ingredient may be mixed with the polymeric deposition aid, the phenol derivative antimicrobial and the surfactant. One or more conventional formulation ingredients may be selected from carriers, preservatives, humectants, emollients and mixtures thereof.

Detailed description of the invention

The present invention provides a method of enhancing the antimicrobial activity of aqueous antimicrobial liquid cleansing formulations comprising a phenol derivative antimicrobial agent and a surfactant or surfactant system.

The method of the present invention comprises mixing a polymeric deposition aid consisting of a liquid, hydroxyl-terminated mixture of urethane polymers in polyethylene glycol with a phenol derivative antimicrobial agent, surfactant or surfactant system As well as at least one additional formulation ingredient mixing step, the antimicrobial activity of the liquid cleansing formulation thus obtained is at least 10% higher than the same formulation without the polymeric deposition aid.

The polymeric deposition aid is a hydroxyl-terminated urethane compound of the formula:

wherein R is selected from alkylene or alkenylene groups containing 1 to about 20 carbon atoms, cycloalkylene or cycloalkenylene groups containing about 5 to about 10 carbon atoms, and containing about 6 to about 10 carbon atoms Atomic, unsubstituted or substituted by one or more lower alkyl, lower alkoxy, nitro or amino or halogen atoms, mononuclear or fused-ring arylene; R' are the same or different alkylene or alkenylene; wherein m is an integer selected from the group consisting of (O-R') moieties having a molecular weight of from about 40 to about 6000; and

wherein n and n' are the same or different integers from 0 to about 30 inclusive, which are related to m such that the hydroxyl-terminated urethane compound has a molecular weight of up to about 200,000. Examples of such polymeric deposition aids are generally described in U.S. Patent 5,051,260, Chess et al., issued September 24, 1991; US Patent 497,080 to Chess et al; these documents are incorporated herein by reference.

Ideally, m is 8 and the values of n and n' are mainly 1-4. It is also desirable that the values of m, n and n' be related so that the molecular weight of the hydroxyl-terminated urethane compound is about 1800.

An example of a polymeric deposition aid is Topicare(R) transfer compound PP-15 (Polyolprepolymer-15) manufactured by Penederm, Inc. of Foster City, California. Polyolprepolymer-15 is a liquid mixture of hydroxyl-terminated polymers in polyethylene glycol. Its CAS name is poly(oxy-1,2-ethanediyl), α-hydrogen-ω-hydroxy-, and 1,1'-methylene-bis-(4,isocyanatocyclohexane) of polymers. The CTFA name is PEG-8/SMDI Copolymer.

The polymeric deposition aid should be water miscible or soluble in water. Although the inventors should not be bound by a particular theory of operation, the water miscibility of the polymeric deposition aid is important to the functionality of the aqueous antimicrobial liquid cleaning formulations of the present invention.

Solubility information for polyolpreploymer-15 (PP-15) from Penederm Inc. is listed in Table 1.

Table 1

Solvent Percent Solubility (%w/w) water Soluble Ethanol (95% SDA 40-2) 50 Isopropanol 50 Propylene Glycol 50 PEG 300 50 Tween 20 50 Benzaze 812 insoluble Silicone (Simethicone) insoluble mineral oil insoluble Ethoxydiglycol 50 glycerin Dispersible

1-PP-15 showed an increase in water solubility as the temperature decreased.

2-When 1.0% or less of PP-15 was added to glycerin, fine droplets were observed under the microscope.

Phenol derivative antimicrobial agents can be selected from 2,4,4'-trichloro-2'-hydroxydiphenylether (also known as triclosan), 3,4,4'-trichlorosymmetric diphenylurea (also known as known as triclocarban), phenoxyethanol, chloroxylenol, o-phenylphenol, and o-phenylphenate. When the phenol derivative antibacterial agent is 2,4,4'-trichloro-2'-hydroxydiphenyl ether, the method of the present invention has a good effect. The content of the phenol derivative antimicrobial agent is usually about 0.1% to about 10% by weight. Desirably, the phenol derivative antimicrobial agent is present in an amount of from about 0.1% to about 3% by weight. More desirably, the phenol derivative antimicrobial agent is present in an amount of about 0.1% to about 1% by weight.

According to the invention, at least one surfactant or surfactant system is mixed with the other ingredients. The surfactant may be anionic, cationic, nonionic and/or amphoteric.

Examples of anionic surfactants include, but are not limited to, ethoxylated alkyl sulfates, alkyl glyceryl ether sulfonates, methyl acyl taurates, fatty acyl glycinates, alkyl sulfosuccinates, Alpha-sulfonated fatty acids, their salts and/or esters, alkyl ethoxy carboxylates and mixtures thereof.

Examples of amphoteric surfactants include, but are not limited to, amphococococarboxypropionate, amphococococarboxypropionate, amphocococoacetate, and amphocococodiacetate. In general, commercially available amphoteric surfactants of this type are produced and sold as electrically neutral complexes with, for example, hydroxide counterions, anionic sulfate or sulfonate esters or salts. Suitable commercially available products include, but are not limited to, those sold under the following trade names: Empigen (Albright &Wilson); Miranol (Rhone-Poulenc); Alkateric (Alkaril Chemicals); Amphoterge (lonza, Inc.); (Rewo Chemical Group) and Schercotic (Scher Chemicals).

The surfactant system may consist of a mixture of surfactants. For example the surfactant system may consist of one or more anionic surfactants together with nonionic surfactants and/or betaine surfactants. A variety of commonly used surfactant systems are commercially available and known to those skilled in the art.

For example, in embodiments of the present invention, at least one nonionic and/or amphoteric surfactant may be mixed with the other ingredients. Of course, nonionic and/or amphoteric surfactant systems may also be used. The surfactant/surfactant system is ideally a nonionic and/or amphoteric surfactant, such a system is mild to the skin and leaves the skin substantially free of redness and dryness, damage to the stratum corneum less sexual. Of course, anionic and/or cationic surfactants may be mixed with nonionic and/or amphoteric surfactants.

Suitable surfactant systems include Miracare MS-1 (available from Rhone-Poulenc) and Standamox CAW (available from Henkel Corp.). Mircacare MS-1 consists of a mixture of PEG80 sorbitan laurate, sodium tridecyl ethoxylate sulfate, PEG150 distearate, and amphoteric lauroyl diacetate in water. StandamoxCAW consists of a mixture of cocamidopropylamine oxide in water. Various other mild surfactants and/or surfactant systems are also contemplated.

Other suitable surfactant systems may include ingredients such as sodium cocoyl isethionate, sodium lauryl sulfate, ammonium sulfate, cocamidopropyl betaine, ammonium lauryl sulfate, PEG 80 sorbitan lauryl Acetate and/or Sodium Tridecyl Ethoxylate Sulfate.

One or more other conventional formulation ingredients may be mixed with the polymeric deposition aid, the phenol derivative antimicrobial and the surfactant or surfactant system. For example, carriers, preservatives, humectants, solvents, etc. can be mixed with conventional formulation ingredients.

Generally, the carrier used in the formulations of the invention is water. Carriers include viscosity regulators, thickeners, colorants, fragrances and/or buffers and/or pH control agents. An example such as a carrier additive is Ucare JR40, which gives the skin a smooth feel after application.

Moisturizers that can be used include, for example, glycerin. The addition of humectants keeps the formulation on the skin hydrated, thereby preventing erythema. Preservatives and preservative enhancers that may be used include, for example, DMDM hydantoin and tetrasodium EDTA.

It is also important for the method of the present invention to distinguish between aqueous antibacterial liquid cleansing formulations for washing and emulsion compositions for cleansing, treating or conditioning the skin.

Generally speaking, an aqueous antibacterial liquid cleansing preparation refers to an antibacterial "liquid soap" containing a cleanser for washing the skin (such as washing hands, bathing or showering, etc.). Such formulations are generally applied to the skin with water or alone, allowed to work into a lather, and rinsed with water. Such cleanser-containing liquid soaps include Lever 2000(R) Antibacterial Liquid Soap (Lever Brothers) and Dial(R) Antibacterial Liquid Soap (Dial Corporation). Often, repeated use of these antibacterial liquid cleansing preparations is prone to erythema and skin irritation.

Conversely, lotion compositions are also commonly used to cleanse, treat and/or condition the skin. Such emulsion compositions are oil-in-water emulsions, which are used to deposit certain ingredients of the oily phase of the emulsion on the skin. These oil-in-water emulsions are often in the form of lotions or lotions. These oil-in-water emulsions are generally considered less prone to erythema and skin irritation, and in some cases, they are actually used to treat skin irritation.

An example of a formulation for practicing the method of the invention may be derived from an aqueous phase, a surfactant phase, a preservative phase and an active ingredient phase, which are mixed together using conventional agitation techniques to form an aqueous antimicrobial liquid cleansing formulation.

The aqueous phase can include sterile deionized water, and can also include additives, such as Ucare JR400.

The surfactant phase contains one or more nonionic or amphoteric surfactants or surfactant systems. It is believed that the surfactant phase may contain small amounts of cationic or anionic surfactants. The surfactant phase may also contain polymeric deposition aids. Ideally, the surfactant phase may comprise surfactant systems such as Miracare MS-1 and Standamox CAW, among others.

The preservative phase may contain glycerin and preservatives and preservative enhancers, such as DMDM hydantoin and tetrasodium EDTA, etc.

The active phase contains a phenol derivative antimicrobial agent and may contain additional nonionic surfactants and fragrances. Ideally, the active phase comprises triclosan as an antimicrobial agent. The nonionic surfactant can be polysorbate 40, NF (available under the tradename Tween 40 from ICI Specialty Chemicals, Wilmington Delaware). An example of a fragrance is Elias Fragrance #16783, available from Elias Fragrance Company.

Typically, the aqueous phase is heated to about 65°C and the surfactant phase is mixed with the aqueous phase with agitation. Then, the preservative phase is added to the mixture with stirring, and finally the active phase is added. Typically the pH is adjusted to 6.5-7 with citric acid and the mixture is stirred well. Examples of formulations according to embodiments of the invention are given in Table 2.

Table 2

Preparation example Element Percent composition (wide range) Percent composition (narrow range) water box Deionized water 20.0-75.0 25.0-35.0 Ucare JR 400 0.05-0.5 0.1-0.25 Surfactant phase Miracare MS-1 20.0-50.0 40.0-50.0 Standamox CAW 2.0-10.0 4.0-6.0 Topicare PP-15 0.5-5.0 1.0-3.0 Amercil 357 0.0-1.0 0.0-1.0 preservative phase glycerin 1.0-10.0 5.0-10.0 DMDM hydantoin 0.4 or on demand 0.4 or on demand Tetrasodium EDTA 0.1 or on demand 0.1 or on demand active phase Triclosan 0.1-1.0 0.5-1.0 Tween 40 1.0-5.0 1.0-3.0 spices 0.0-0.3 0.0-0.1

According to the present invention, these aqueous antimicrobial liquid cleansing formulations have at least 10% (eg, 20% or more) higher antimicrobial activity than system formulations without polymeric deposition aids.

Example

preparation

The following examples describe aqueous antimicrobial liquid cleansing formulations. Generally, ingredients are identified by chemical name, CFTA name or, in some cases, trade name. The ingredients are combined using conventional mixing and/or soap making techniques. The specific contents of each component of Examples 1-5 are shown in Table 3.

table 3

percent composition Example 1 Example 2 Example 3 Example 4 Example 5 water box Deionized water 30.2 29.2 29.2 27.2 25.2 Ucare JR400 0.2 0.2 0.2 0.2 0.2 Surfactant phase Miracare MS-1 50.0 50.0 50.0 50.0 50.0 Standamox CAW 5.0 5.0 5.0 5.0 5.0 Topicare PP-15 0.0 0.0 1.0 3.0 5.0 Amercil 357 0.0 1.0 0.0 0.0 0.0 preservative phase glycerin 10.0 10.0 10.0 10.0 10.0 DMDM hydantoin 0.4 0.4 0.4 0.4 0.4 Tetrasodium EDTA 0.1 0.1 0.1 0.1 0.1 active phase Triclosan 1.0 1.0 1.0 1.0 1.0 Tween 40 3.0 3.0 3.0 3.0 3.0 spices 0.1 0.1 0.1 0.1 0.1

The ingredients are generally mixed using conventional techniques. The aqueous phase was prepared by adding polymer Ucare JR 400 to deionized water at room temperature. Generally speaking, there should be enough time for the polymer Ucare JR 400 to disperse (eg about 10 minutes). The aqueous phase was then heated to 65°C.

Prepare the surfactant phase, preservative phase, and active phase by premixing them separately in three separate containers. The surfactant phase was prepared by mixing Miracare MS-1, Standamox CAW and Topicare PP-15. At this point, other optional ingredients, such as Amercil 357, can be added to the surfactant phase. Thus, the surfactant phase contains the polymer transfer aid and the surfactant.

Prepare the preservative phase by mixing glycerin, DMDM hydantoin and tetrasodium DETA.

The active phase was prepared by mixing triclosan with Tween 40 and fragrance.

After the water phase reached 65°C, the surfactant phase was added to the water phase with slow stirring.

The temperature of the combined aqueous and surfactant phases was maintained at 50°C while the preservative phase was added under agitation.

Next, the temperature of the combined aqueous, surfactant, and preservative phases was maintained at 40°C while the active phase was added under stirring.

The pH of the mixture was checked and adjusted to 6.5-7 by adding a small amount of 5% citric acid. The mixture was stirred at high speed overnight, during which time the temperature was cooled to room temperature.

Antibacterial activity

Determination of the antimicrobial activity of aqueous antimicrobial cleaning aids. Their antimicrobial effects were compared to control formulations and regular liquid soaps with and without antimicrobial ingredients.

Antimicrobial activity was determined in R.O.D.A.C. (parallel microbial assay and enumeration) plates. These dishes are 65 x 15 mm dishes specially designed for the raised surface of the medium. Lecithin and Tween 80 were added to the medium to inactivate residual chemicals on hands that might interfere with microbial growth in the dish.

The three media are: Trypticase Soy Agar (TSA), MacConkey Agar (MAC) and Sabouraud Dextrose Agar (SDA). Each medium contained 0.7 g/L lecithin and 5.0 g/L polysorbate 80.

Gram-positive bacteria that may be present on the thumb were cultured with TSA medium. Gram-negative bacteria that may be present on the middle finger were cultured with MAC medium. Yeasts and molds that may be present on the palms were cultivated with SDA medium.

The method is: 1) contact the target area with the specified R.O.D.A.C. medium to obtain an initial microbial count; 2) wet and wash your hands for 1 minute, then dry with a paper towel; and 3) contact the target area with the specified R.O.D.A.C. medium To obtain microbial counts after cleaning. Calculate the percent microbial reduction by subtracting the Step 3 specific count from the Step 1 count and dividing by the Step 1 count. This procedure was repeated for several testers, and the average value was calculated.

The liquid cleaning formulations of Examples 1 and 3 were tested with the following commercially available liquid soaps: Dial® antibacterial soap, Lever 2000®, Operating Room Scrub, containing 1.25% p-chloro-m-xylenol (PCMX ) of Sanifresh Soap. Three non-antibacterial soaps were also tested: Softsoap(R), Sanifresh Premium and Eurobath(R). Table 4 reports the results.

Table 4 experiment material Percentage reduction in microbial colony count (total) Percent reduction in microbial colony count (Gram-negative bacteria only) Example 3 60 55 Example 1 0 0 Dial® soap 40 38 Lever 2000® 28 20 OR Scrub 45 50 Sani-Fresh1.25%PCMX 50 62 Softsoap® 0 0 Sani-Fresh Premium 0 0 Eurobath® 0 0

The effect of adding polymeric transfer aids to conventional liquid antibacterial soap formulations was investigated using the R.O.D.A.C (Recurrent Microbial Determination and Enumeration) plate test described above (using the same data reporting method). Dial(R) liquid antibacterial soap was used as a control. The formulation tested was Dial(R) liquid antibacterial soap containing 3% by weight of Topicare(R) transfer compound PP-15. Table 5 reports the results.

table 5 experiment material Kill % (bacteria) Kill % (Yeast/Mold) Dial® soap 55 33 Dial® Soap + 3% PP-15 65 53

The effect of adding polymeric transfer aids to aqueous antimicrobial liquid cleaning formulations containing mild surfactants that reduce antimicrobial activity was investigated using the R.O.D.A.C (Recurrent Microbiological Determination and Enumeration) plate test described above (using the same data reporting method) . The formulation of Example 1 was used as a control. The formulation tested was the formulation of Example 3 containing 1.0% by weight of Topicare(R) transfer compound PP-15. Table 6 reports the results. The percent reduction in microbial colony count reported in Table 6 was calculated as described above, except that negative values were averaged at zero. The percent colony reduction was higher for formulations that were less effective in microbial growth than in reduction. Averages calculated in this way are conservative comparisons to products that perform well (ie, have a greater reduction in microbial growth).

Table 6 microorganism Example 1 Percentage reduction Example 3 Percentage reduction Gram-positive bacteria 38 50 Gram-negative bacteria 18 39 Yeast/Mold 16 39

The antimicrobial effect of the commercially available Lever 2000(R) liquid antibacterial soap and the formulation of Example 3 was compared using the R.O.D.A.C (Parallel Microorganism Determination and Counting) plate test. Table 7 reports the results. The percent microbial colony reduction reported in Table 7 was calculated as described above, with negative numbers included in the calculation of the mean.

Table 7 microorganism Percentage reduction of Lever 2000® Example 3 Percentage reduction Gram-positive bacteria 35 50 Gram-negative bacteria 30 39 Yeast/Mold 34 39

As can be seen from the data reported in Tables 5 and 7, the present invention provides a means of enhancing the antibacterial activity of conventional antibacterial soap formulations, such as Dial(R) Antibacterial Liquid Soap and Lever 2000(R) Liquid Soap. The percentage reduction in the number of microbial colonies can be increased by 10% or more using the method of the present invention. For example a 20% increase. In some cases, the improvement can be as high as 40% or even 60% or more.

The data in Tables 4 and 6 demonstrate that the present invention provides a mild liquid cleansing formulation with acceptable levels of antimicrobial activity. The mild liquid cleansing formulation has lower antimicrobial activity without the addition of a polymer transfer aid. In fact, the data in Table 4 show that the formulation without the polymer transfer compound had essentially no measurable antimicrobial activity.

While the invention has been described using certain embodiments, it should be understood that the inventive subject matter should not be limited to the specific embodiments. On the contrary, the subject matter of the invention should include all the various changes, modifications and equivalents falling within the spirit and scope of the invention.

Claims (17)

1, a kind of method that strengthens the anti-microbial activity of moisture antibacterial liquid cleaning formulation, this method comprise mixing by liquid state, hydroxyl is the polymer deposition auxiliary agent formed of the mixture of polymkeric substance in polyoxyethylene glycol of the urethane of end group and the step of phenol derivatives antiseptic-germicide and at least a tensio-active agent, the anti-microbial activity of the liquid cleaning formulation that so obtains is than the same preparation height at least 10% that does not contain the polymer deposition auxiliary agent.

2, the polymer deposition auxiliary agent that the process of claim 1 wherein be following formula be the urethane compound of end group with the hydroxyl:

Wherein R is selected from alkylidene group or the alkylene group that comprises about 20 carbon atoms of 1-, the cycloalkylidene or the inferior cycloalkenyl group that contain about 10 carbon atoms of about 5-contain the monokaryon about 10 carbon atoms of about 6-, that do not replace or replaced by one or more low alkyl groups, lower alkoxy, nitro or amino or halogen atom or the arylidene of fused rings;

Wherein R ' is identical or different alkylidene group or alkylene group;

Wherein m is selected from and makes the integer of the molecular weight of (O-R ') part for about 40-about 6000;

Wherein n and n ' are that they are relevant with m from the 0-identical or different integer of about 30 (comprising 30), so that hydroxyl is that the molecular weight of urethane compound of end group is up to about 200,000.

It is the integer of about 1-about 8.

3, the polymer deposition auxiliary agent that the process of claim 1 wherein is poly-(oxygen-1,2-ethane two bases), α-hydrogen-ω-hydroxyl-, with 1, the polymkeric substance of 1 ' methylene radical-two-(4, the isocyanato-hexanaphthene).

4, the method for claim 1, wherein the polymer deposition auxiliary agent be liquid be the mixture of urethane polymkeric substance in polyoxyethylene glycol of end group with the hydroxyl, the molecular-weight average of the polymkeric substance in the described mixture is about 1800 and comprises 1,1 '-methylene radical-two-(4, the isocyanato-hexanaphthene) part.

5, the phenol derivatives antiseptic-germicide that the process of claim 1 wherein is 2,4,4 '-three chloro-2 '-hydroxy diphenyl ether.

6, the process of claim 1 wherein that at least a tensio-active agent is selected from anion surfactant, cats product, nonionogenic tenside, amphoterics and their mixture.

7, the method that the process of claim 1 wherein also comprises the step that adds at least a conventional preparation composition.

8, the method for claim 7, at least a routine wherein are mixed with sorting from carrier, sanitas, wetting Agent for Printing Inks, softener and their mixture.

The anti-microbial activity of the liquid cleaning formulation that 9, the process of claim 1 wherein is than the same preparation height at least 20% that does not contain the polymer deposition auxiliary agent.

10, a kind of method that strengthens the anti-microbial activity of moisture antibacterial liquid cleaning formulation, this method comprise mixing by liquid state, hydroxyl is the polymer deposition auxiliary agent formed of the mixture of polymkeric substance in polyoxyethylene glycol of the urethane of end group and the step of phenol derivatives antiseptic-germicide and at least a tensio-active agent, the anti-microbial activity of the liquid cleaning formulation that so obtains is than the same preparation height at least 20% that does not contain the polymer deposition auxiliary agent.

11, the method for claim 10, polymer deposition auxiliary agent wherein be following formula be the urethane compound of end group with the hydroxyl: Wherein R is selected from alkylidene group or the alkylene group that comprises about 20 carbon atoms of 1-, the cycloalkylidene or the inferior cycloalkenyl group that contain about 10 carbon atoms of about 5-contain the monokaryon about 10 carbon atoms of about 6-, that do not replace or replaced by one or more low alkyl groups, lower alkoxy, nitro or amino or halogen atom or the arylidene of fused rings;

Wherein R ' is identical or different alkylidene group or alkylene group;

Wherein m is selected from and makes the integer of the molecular weight of (O-R ') part for about 40-about 6000;

Wherein n and n ' are that they are relevant with m from the 0-identical or different integer of about 30 (comprising 30) so that hydroxyl be the molecular weight of urethane compound of end group up to about 200,000,

It is the integer of about 1-about 8.

12, the method for claim 10, polymer deposition auxiliary agent wherein are poly-(oxygen-1,2-ethane two bases), α-hydrogen-ω-hydroxyl, and with 1, the polymkeric substance of 1 '-methylene radical-two-(4, the isocyanato-hexanaphthene).

13, the method for claim 10, wherein the polymer deposition auxiliary agent be liquid be the mixture of urethane polymkeric substance in polyoxyethylene glycol of end group with the hydroxyl, the molecular-weight average of the polymkeric substance in the described mixture is about 1800 and comprises 1,1 '-methylene radical-two-(4, the isocyanato-hexanaphthene) part.

14, the method for claim 10, phenol derivatives antiseptic-germicide wherein is 2,4,4 '-three chloro-2 '-hydroxy diphenyl ether.

15, the method for claim 10, wherein at least a tensio-active agent is selected from anion surfactant, cats product, nonionogenic tenside, amphoterics and their mixture.

16, the method for claim 10, method wherein also comprise the step that adds at least a conventional preparation composition.

17, the method for claim 16, at least a routine wherein are mixed with sorting from carrier, sanitas, wetting Agent for Printing Inks, softener and their mixture.