CN1213762C - Medicine for invigorating kidney, promoting blood circulation and promoting skeleton formation and its prepn - Google Patents
Medicine for invigorating kidney, promoting blood circulation and promoting skeleton formation and its prepn Download PDFInfo
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- CN1213762C CN1213762C CN 02128467 CN02128467A CN1213762C CN 1213762 C CN1213762 C CN 1213762C CN 02128467 CN02128467 CN 02128467 CN 02128467 A CN02128467 A CN 02128467A CN 1213762 C CN1213762 C CN 1213762C
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Abstract
The present invention relates to medicine for tonifying kidneys, activating blood circulation and growing bones, which is prepared from icariin selenium, lycium barbarum polysaccharide selenium, pilose antler polysaccharide, white peony glycoside, astragalus root glycoside, angelica extractum, tanshinone, matrine selenium, licorzinc, vitamin E and vitamin D. Medicine provided by the present invention can cure diseases such as deficiency of both qi and blood, stagnation of qi and blood stasis, insufficiency of the liver and the kidney, bone injury, necrosis of the bone caused by the lack of blood, damage of the tendon and the vein, delay of fracture union, etc. The present invention is combined treatment medicine.
Description
Technical field
The present invention relates to a kind of medicine of invigorating kidney, promoting blood circulation and promoting skeleton formation.
Background technology
Ischemic necrosis of fermoral head is difficult to treatment, and traditional method generally adopts the medicine partner treatment of effects such as blood circulation promoting and blood stasis dispelling, anti-inflammatory analgetic, bone growth promoting granulation promoting, but effect is bad, tends to cause osseous tissue, vascular tissue's neurocyte pathological changes and is difficult to control.Also do not have a kind of good effect at present, the medicine with Comprehensive Treatment effect is well treated ischemic necrosis of fermoral head.
Summary of the invention
The object of the present invention is to provide and a kind ofly both can treat diseases such as QI and blood deficiency, qi depression to blood stasis, deficiency of the liver and kindey, can treat the Comprehensive Treatment medicine of diseases such as bone injury femoral head ischemia necrosis, tendon and vessel injury, slow fracture union again.
The present invention realizes by following technological means:
A kind of medicine of invigorating kidney, promoting blood circulation and promoting skeleton formation, it consists of icariine selenium: 40-60mg/kg, lycium barbarum polysaccharide: 2-4mg/kg; Pilose antler polysaccharides: 80-100mg/kg; Albiflorin: 20-35mg/kg; Astragalin: 26-32mg/kg; Eumenol: 26-32mg/kg; TANSHINONES: 26-32mg/kg; Matrine selenium: 8-12mg/kg; Zinc glycyrrhizate: 18-22mg/kg; Vitamin E: 4-6mg/kg; Vitamin D: 1-2mg/kg; Add up to 251-337mg/kg.
The preparation method of the medicine of this invigorating kidney, promoting blood circulation and promoting skeleton formation, concrete preparation technology is as follows:
(1) icariine selenium extraction process:
(1) crude product icariine selenium extraction process: the former medicine of rough lumber adds 8 times of amount 1% selenic acid liquid, kept 80 ℃ of reflux, extract, 3 hours, filtering the extraction medicinal liquid puts in addition, residue adds 5 times of amount 1% selenic acid liquid again, pressing the Same Way operation extracted 2 hours, medicinal liquid is extracted in multiple filter and preceding liquid merges, and gathers all selenic acid extracting solution, is condensed into than the 1.1-1.2 that weighs, add 95% ethanol equivalent precipitated impurities, left standstill 12-14 hour, and extracted supernatant liquid filtering, precipitation is with 45% washing with alcohol 2-3 time, the solubility composition washes out in will precipitating, filtrate and washing liquid merge, reclaim ethanol, concentrating under reduced pressure is cooled to cream, promptly gets the thick extracted extract of Herba Epimedii selenium;
(2) pure product icariine selenium extraction process: the thick extracted extract of Herba Epimedii selenium adds 70%4 times of amount ethanol, and heating and refluxing extraction 2 hours is filtered, and filtering residue adds 2 times of amount alcohol reflux 1 hour again, filters, and reclaims ethanol, and 60 ℃ of dryings promptly get pure product icariine selenium;
(2) lycium barbarum polysaccharide extraction process:
Getting former medicinal 1% zinc sulfate, 45% ethanol of Fructus Lycii moistening 4 hours, is solvent dipping 24 hours with 1% zinc sulfate, 45% ethanol, with the speed seepage of per minute 50ml/5kg, when making transudate reach 7 times of amounts of crude drug, promptly stop seepage, collection is got sepage, reclaim ethanol, and thickening filtration; Be adjusted to every milliliter and contain total amount 150mg, add 0.25% benzoic acid again, 0.1% nipalgin is anticorrosion, leaves standstill 8-10 days, gets the supernatant and filters, and, is dried into dry extract that is;
(3) zinc glycyrrhizate extraction process:
(1) thick zinc glycyrrhetate extraction process: thick Radix Glycyrrhizae powder is measured 0.5% zinc carbonate (ZnCO with 12 times
3) soak seepage after 12 hours, continuous 3 times, effusion is concentrated into original volume 1/5 and filters, and filtrate adds concentrated sulphuric acid to PH3, filters the precipitation of separating out, and washes 3 times, and is dry below 60 ℃, promptly gets thick zinc glycyrrhetate;
(2) pure product zinc glycyrrhizate extraction process: above gained zinc glycyrrhizate 400g adds 4 times of amount ethanol, reflux 3 hours, filter, 2 times of amounts of filtering residue alcohol heating reflux 2 times merges all backflow and adds the 10g active carbon, reflux decolour cooling half an hour, filter, filtrate ammonification controlled atmosphere is to PH7-7.5, till no solid is separated out, the filtrate precipitation, with the small amount of ethanol washing, drying at room temperature gets zinc glycyrrhetate 250g;
(4) matrine selenium extraction process:
(1) extraction process: Herba Sophorae alopecuroidis or root pulverize to coarse powder, add 5 times of amounts of 1% manganese sulfate, 75% ethanol reflux inclined and supernatant in 1 hour, the same method of medicinal residues adds 70% ethanol, 1% manganese sulfate heating and refluxing extraction 2 times again, merge 3 times extracting solution, quiet amount 1-2 days, filter, filtrate recycling ethanol, to do not have the alcohol flavor, be condensed into cream, the oven dry back becomes dry extract standby;
(5) Cornu Cervi Pantotrichum extraction process:
Get Cornu Cervi Pantotrichum, be sawn into the 3-4mm segment, steamed about 20 minutes, peel off fur, be split into 4-5 millimeters thick fritter; Fine and soft and 5 times of amounts of 5% ethanol add in the backflow extractor together, and water-bath refluxed 4 hours, collect backflow, and 2-3 room filtered through gauze is collected filtrate, place below 10 ℃ more than 24 hours, and fine and soft slag is used with method extraction 5 times, and merging filtrate is condensed into cream, is dried into cream for 50 ℃;
(6) astragalin extraction process:
Radix Astragali decoction pieces, decocting boils secondary, each 1.5 hours, extract water liquid concentrating under reduced pressure, concentrated solution adds 95% ethanol makes determining alcohol reach 60%, the leaching precipitate, be dissolved in water, the centrifugal insoluble matter of removing, filtrate decompression is concentrated into stock solution 1/5, add 95% ethanol again and make determining alcohol reach 80%, stand at low temperature is spent the night, and inclining supernatant, heavy filter part adds high concentration ethanol and stirs, and filters washing with alcohol, vacuum drying gets thick astragalus polysaccharides; Again water-soluble, to filter, filtrate is by the DEAE anion-exchange column, washing is collected, astragalin stream part of a speckle of electrophoretic examinations, merge and concentrate, with the reverse flowing water dialysis of dialyzer 24 hours, to take out and remove by filter a small amount of precipitate, filtrate decompression concentrates, add 95% ethanol precipitation, filter, use absolute ethanol washing, promptly in five oxidations, two carbon vacuum dryings;
(7) TANSHINONES extraction and separation process:
Get the Radix Salviae Miltiorrhizae crude drug powder, 95% ethanol heat is carried, and pure extractum refluxes with an amount of benzene, and benzene liquid is mixed on aluminium oxide after concentrating, and carries out alumina column chromatography, and with the benzene flushing, the benzene eluent is concentrated slightly, separates out column crystallization, is TANSHINONES;
(8) albiflorin extracts:
Radix Paeoniae Alba is given birth to coarse powder, presses percolation, with 30% ethanol moistening 4 hours, with 30% alcohol is solvent dipping after 48 hours, with the speed percolation of per minute 50ml/50kg, when the transudate of waiting reaches 5 times of crude drugs, both stopped percolation, collect sepage, steam back alcohol, and add concentrated, filter, after getting a little orange clear filtrate mensuration content, be adjusted to every milliliter and contain total extract 100mg, add 0.2% benzene again; Formic acid is anticorrosion to leave standstill 48 hours, got supernatant liquid filtering, concentrated, and oven dry promptly;
(9) Radix Angelicae Sinensis extraction process:
The Radix Angelicae Sinensis fragment decocts with water three times, merges decoction liquor, evaporation and concentration liquid adds 95% ethanol makes pure content reach 65%, refrigerator was placed 24 hours, and filtered and recycled ethanol is concentrated into 1: 2, adding 95% ethanol again makes and contains alcohol amount and reach 80%, refrigerator was placed 24 hours, and filtered and recycled ethanol adds ammonia and transfers pH value to 8-8.5, placed refrigerator interior 24 hours, it is standby that filtering ammonia is condensed into cream excessively;
(10) preparation process:
Icariine selenium: 40-60mg/kg/ day, lycium barbarum polysaccharide: 2-4mg/kg/ day; Pilose antler polysaccharides: 80-100mg/kg/ day; Albiflorin: 20-35mg/kg/ day; Astragalin: 26-32mg/kg/ day; Eumenol: 26-32mg/kg/ day; TANSHINONES: 26-32mg/kg/ day; Matrine selenium: 8-12mg/kg/ day; Zinc glycyrrhizate: 18-22mg/kg/ day; Vitamin E: 4-6mg/kg/ day; Vitamin D: 1-2mg/kg/ day; Amount to 251-337mg/kg/ day, be divided into 3 packed, every bag of 83.67-112.33mg.
Described Cornu Cervi Pantotrichum extraction process also can for: remove the skin Cornu Cervi Pantotrichum and be cut into small pieces, with 50% alcohol reflux and filtering and concentrating liquid with Different concentrations of alcohol deproteinization 5 times, filtrate being diluted to contains 60% of crude drug in whole, sterilization, freezing, filter, add repeatedly the activated carbon filtration heating, freezing, concentrate and be dried into cream.
Described astragalin extraction process also can for: get Radix Astragali 10kg and add injection water and soak half an hour, decoct each 1 hour 3 times, filter, merging filtrate is concentrated into 5000ml, add alcohol and reach 60%, stir, placed 40 hours to containing the alcohol amount, filter, reclaim concentration of alcohol to 2000ml, adding concentration of alcohol again is more than 80%, placed 40 hours, and filtered, reclaim ethanol, being concentrated into does not have the alcohol flavor, is condensed into cream, dry for standby.
Described TANSHINONES extraction process also can for: round reason and clean Radix Salviae Miltiorrhizae, with decocting in water three times, filter, merge three times decocting liquid, be concentrated into every milliliter and contain crude drug 1.5-2g, add 95% ethanol and make medicinal liquid contain alcohol amount to reach 85%, places filtrations in 4 hours, concentrated recovery ethanol also is concentrated into every milliliter and contains raw material 3-5g, adding 95% ethanol again makes medicinal liquid contain crude drug to reach 85%, placed 4 hours, filtrate recycling ethanol is concentrated into every milliliter and contains crude drug 3-5g, pours 13 times of amount distilled water, place filtration in 24 hours, filtrate is concentrated into every milliliter and contains crude drug 3-5g, and reuse 95% ethanol precipitation once makes medicinal liquid contain the alcohol amount and reaches 80%, place filtration in 24 hours, filtrate recycling ethanol is not to there being the alcohol flavor, and dense one-tenth cream is dried into powder.
The present invention has following effect:
One, medicine characteristics
This medicine has novelty, and reasonable recipe has the minimum characteristics of high-efficiency low-toxicity side effect.This medicine has very strong vigorate qi and replenish the blood nourishing the liver and kidney, promoting blood circulation to remove blood stasis, relaxing muscles and tendons to promote blood circulation, anti-inflammatory, repercussive and analgesic, bone and muscle strengthening, the strong waist of spermatogenesis, the long marrow of the kidney invigorating.Promote the fracture and injury healing, increase femur blood capillary blood flow, make flesh and blood liquid, vitamin, trace element, bone desired nutritional material is sufficient to be supplied with.Reverse ischemic necrosis of femoral head and recover.Osseous tissue, vascular tissue, nervous cell regenerating and rehabilitation.Paraplegia, cranial nerve, spinal nerves due to promotion and the acceleration miscellaneous fracture, upper and lower limb nerve, blood vessel, damage, Regeneration and Repair and rehabilitation as early as possible.Promote DNA and protein synthesis, normal bone growth and impaired, the strong shape of necrotic bone recovery.And very strong immunosuppressant and immunological enhancement, anti-inflammatory, repercussive and analgesic effect, very strong solution toxic action arranged.Mainly be applicable to QI and blood deficiency, qi depression to blood stasis, deficiency of the liver and kindey, fracture, Avascular Necrosis Of Femur Head, tendon and vessel injury, bone and arthritis, damage, change deformity, swell and ache, heal slow etc. that the extra-heavy facilitation is arranged: (1) is efficient: according to 462 routine Avascular Necrosis Of Femur Head case clinical treatment observations: take " invigorating kidney, promoting blood circulation and promoting skeleton formation medicine ".Cure 400 examples and account for 86.58%: produce effects 29 examples account for 6.28%, and effective 23 examples account for 4.98%, total effective rate 97.84％。(2) quick-acting: the people who takes invigorating kidney, promoting blood circulation and promoting skeleton formation medicine 5-10 day 92.86% can reach pain relieving, and remission rate 86.58% in 10 days.(3) low toxicity: the clinical treatment Avascular Necrosis Of Femur Head was taken 3 months, and after testing: the heart, liver, kidney, reproductive system organ pipe are all harmless.Just one or two people have gastrointestinal reaction, formerly have the inclination, liver, nephropathy change person, and one or two people respond.
1. this medicine medicine extraction process advanced person, the purity height is so toxic and side effects is little: compare curative effect with similar medicine and improve 300%.Particularly the last skill Artemisia of snow is used high temperature, high pressure, dimension secondary element, acid, alkali, temperature control novel technology for extracting.Biological alkali (acetyl benzaconine, mesaconitine, inferior acetyl benzaconine, jesaconitine, the delphisine) toxicity of two fat type Diterpeneses greatly＞through the biological alkali of high-temperature high-pressure acidic hydrolysis one-tenths → monoesters class (stupid formyl aconine is Aconitum carmichjaelii Debx. time alkali, stupid formyl mesaconitine both, stupid formyl time acetyl benzaconine) toxicity less (being the toxic 1/500-1/1000 of di esters biology alkali).Again through biological alkali (aconine is aconine, mesaconitine, hypo-aconine both) the toxicity minimum of High Temperature High Pressure acidic hydrolysis one-tenth → alcamines, (toxicity is two lipids alkaloidal 1/2000) is so toxicity is extremely low, clinical practice is very safe, the effect of rheumatism stopping numbness pain is better than salicylic acid inferior to morphine.Wind-damp dispelling antiinflammatory action and nonsteroidal are suitable.The biological inspection 0 of Aconitum carmichjaelii Debx..The analgesic effect of 1mg/kg non-6mg/mk effect is also strong.
2. kidney invigorating and YANG supporting, bone and muscle strengthening, elimination inflammation of bone, promote osteogenesis: this medicine can promote sexual function: after, seminal vesicle hyperfunction owing to the promotion gonepoiesis is full of, sensation nerve, indirect excitation libido and cause, can increase the prostate seminal vesicle, levator ani weight, promote testis and epididymis to grow sexual function improving.Strengthen women uterus, ovary weight, postponement climacteric.Anti-aging effects: prolong normal cell survival, anti-hypoxia, resisting fatigue.Eliminate sclerotin and arthritis, promote osteogenesis and rheumatism.
3. strong immunosuppressant and antiinflammatory action: suppress immunoreation, again immunological enhancement arranged.Humoral immunization, immunization of cell, dual regulation are arranged.Can induce r-interferon (1FN-r), interleukin II (1L-2), interleukin (1L-3), Z tumor necrosis factor (TNF-Z), B-transforming factor (TGF-B1), HIV-1 inhibitive factor, natural killer cell (NK cell).These immunity active substances, to regulating immunity, suppress tumor, suppressing HIV remarkable effect is arranged.Treatment to connective tissue disease and immune disease has obtained effect unique especially.A variety of causes is caused that the granuloma hypertrophy has inhibitory action, reduce histamine, capillary permeability, increase the phagocytic function of inflammation emigrated cell, have very strong anti-inflammatory properties.
4. this medicine has very strong Detoxication: in this medicine, the biological alkali hyoscyami of Radix Physochlainae extract, atropic category alkali, can remove Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) to the direct infringement of cardiac muscle and cardiac dysfunction, rhythm abnormality etc.Radix Glycyrrhizae is extracted glycyrrhizic acid also can remove Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) to the infringement of important organs such as the heart, liver, kidney, brain, heart is had protective effect and Detoxication, and can remove the acetyl benzaconine class endocrine reproductive system is encroached on.Glycyrrhizic acid and Herba Epimedii have dual regulation according to dosage to man, female reproductive system system.
5. have very strong antibiotic, antifungal, antivirus action: can suppress golden Portugal bacterium, streptococcus, fungus and virus, high concentration has killing action.HIV there is the very strong effect that presses down, and can suppresses hiv reverse transcriptase.To the microbial rheumatic fever of hammer, bring down a fever, pain relieving, detumescence effect extra-heavy.
6. vasodilation, vein effect are arranged, blood pressure lowering, heart tonifying diuresis are arranged: and the energy anticoagulant, thrombosis prevented and treated.Low dosage has good cardiotonic, antiarrhythmic effect.
7. the bone and its cells of enhancing hypoxia-bearing capability is arranged: prolong the normal cell survival time-to-live, promote protein and nucleic acid metabolism, promote the normal bone growth, osteitis, hyperosteogeny are controlled in harm, degenerative osteoarthropathy takes place and development, and can prevent and treat immunoreation and cause osteitis and osteonecrosis.
8. this medicine medicine source is wide; cheap and curative effect height, toxic and side effects be little, be convenient to promote the use of: because this medicine has above such innovation novel technology for extracting; reasonable formula; so advantages such as high-efficiency low-toxicity are applied for a patent and are protected; enlarge our production scale; had more product, for Chinese people and national people of the world's hygiene and health cause are done contribution more.
Two, invigorating kidney, promoting blood circulation and promoting skeleton formation medicine pharmacological action
1. endocrine function is had and promote and potentiation: (1) can promote gonepoiesis, and prostate, essence and levator ani weight are increased.(2) urge excessive effect: make gonepoiesis hyperfunction, the neural and excited libido of sensation.(3) generation of promotion male's testosterone (TS) secretion and CAMP.(4) can improve the reactivity of hypophysis to luteinizing hormone releasing hormone LRH to the women, and the reactivity worth that corpus luteum generates plain LH obviously increases antepituitary, ovary, uterus weight, after taking medicine, ovary HCG, LH receptor-specific improve 3 times.
2. this medicine has potentiation to immunologic function: to kidney deficiency human T-cell number, pouring rate of rotation, antigen and reticuloendothelial system phagocytic hypofunction person, the every index in clothes " the kidney warming rheumatism miraculous cure " back is improved, Histological change and minimizing extracellular matrix to kidney produce, and immunity of organism is had facilitation.Low concentration can increase Ea bow structure rate, and the effect of low concentration enhance immunity is described, high concentration suppresses immunization, and immunity is had dual regulation.
3. immunoglobulin is influenced: immunoglobulin (1g) has harmful substances invasion and attack such as antagonism alien bacteria, virus, various toxin, keeps biological self stability and various important function such as immune surveillance.The patient measures its value under normal value at take medicine preceding 1gG, 1gA, and clothes " the kidney warming rheumatism miraculous cure " back 1gG, 1gA content obviously increase.It is the life-prolonging performance that old people 1g increases.
4. the contained flavonoid composition of this medicine is to the enhancing and the inhibitory action of immunologic function, and dual regulation: total flavones can improve the serum hemolysin antibody horizontal, increases the antibody-producting cell number, promotes to drench rate of rotation, increases macrophage function.High concentration can press down the breeder reaction of lymphocyte to ConA, LPS, mixed lymphocyte reaction strongly.Total allusion quotation ketone suppression ratio and drug level are proportional.Simultaneously can prolong the allogeneic mouse heart transplanting survival phase.
5. this medicine polysaccharide, hyoscyami alkali class, glycyrrhizic acid, Herba Sophorae alopecuroidis alkali etc., biological alkali class can suppress the destruction of Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) to cardiovascular system hemopoietic system, marrow hemopoietic stem cells.Make spleen lymphocyte proliferation, produce interleukin II (IL-2), polysaccharide has the effect of the immunne response of promotion, can increase the immunological memory function simultaneously.And can increase luring property of ConA r-interferon (IFN-r) and IL-2.
6. this medicine has the steroid hormone effect: and can stimulate hypothalamus-hypophysis-hypothalamic pituitary adrenal axis excitement, and impel the secretion of adrenal cortex releasing hormone (CRH) to increase, or optionally excited suprapituitary crh receptor, indirect action is in the adrenal gland and secreting hormone.Effect is played in the increase of adrenocortical hormone content in the body: (1) antiinflammatory action.(2) immunosuppressive action.(3) antitoxic action.(4) Antishock function.(5) to metabolic effect.(6) to lymphocyte and blood cell effect.
7. this medicine has very strong immunosuppressive action and antiinflammatory action: analgesia, analgesic, calm, the effect of separating reach 93 to rheumatic arthritis 3-7 days pain relieving rates.The detumescence rate reaches 85 in 45%, 7 day.65%, cure rate reaches 85.65%, obvious effective rate reaches 7.85%, effective percentage reaches 4.93%, total effective rate 98.43%, anti-chain " 0 " negative conversion rate 96%, the rheumatoid factor negative conversion rate reaches 76.6％。
8. antitumor action and anti-HIV effect: this medicine can suppress ConA and LPS to lymphocytic propagation, suppresses and kills the tumor target cell.It is to suppress PHA to induce down transcribing of cell IL-2mRNA and IL-2RP55mRNA that HIV propagation is had very strong inhibitory action, Herba Sophorae alopecuroidis alkali and glycyrrhizin, and inhibitory action is induced as for end effect in 12 hours the strongest at PHA.Herba Sophorae alopecuroidis alkali and glycyrrhizin are to CD
4+ cell CD
8+ with cell obvious inhibitory action is arranged all, to CD
4+ cyto-inhibition is the strongest.
9. sick pharmacology such as this Drug therapy rheumatic arthritis, rheumatoid arthritis, osteitis and sliding matter hypertrophy: the specific antibody that this medicine suppresses the anti-sheep red blood cell (SRBC) of antigen binding cell and antibody secreting cell produces, blocking-up class wind shows the synthetic feedback approach of 1gG antibody in arthritic's body, and disease is eased.Also can make immunoreation trend hyperfunction in the body normal, clothes " the kidney warming rheumatism miraculous cure " back patients serum 1gG, 1gM, 1gA all reduce, and anti-chain " 0 " rheumatoid factor (RF) titre of turning out cloudy lowers or turns out cloudy, and illustrates that this medicine has strong inhibition antibody nucleus formation.
Specific embodiment
Embodiment one:
The preparation technology of the medicine of this invigorating kidney, promoting blood circulation and promoting skeleton formation is as follows:
(1) icariine selenium extraction process:
(1) crude product icariine selenium extraction process: the former medicine of rough lumber adds 8 times of amount 1% selenic acid liquid, kept 80 ℃ of reflux, extract, 3 hours, filtering the extraction medicinal liquid puts in addition, residue adds 5 times of amount 1% selenic acid liquid again, pressing the Same Way operation extracted 2 hours, medicinal liquid is extracted in multiple filter and preceding liquid merges, and gathers all selenic acid extracting solution, is condensed into than the 1.1-1.2 that weighs, add 95% ethanol equivalent precipitated impurities, left standstill 12-14 hour, and extracted supernatant liquid filtering, precipitation is with 45% washing with alcohol 2-3 time, the solubility composition washes out in will precipitating, filtrate and washing liquid merge, reclaim ethanol, concentrating under reduced pressure is cooled to cream, promptly gets the thick extracted extract of Herba Epimedii selenium;
(2) pure product icariine selenium extraction process: the thick extracted extract of Herba Epimedii selenium adds 70%4 times of amount ethanol, and heating and refluxing extraction 2 hours is filtered, and filtering residue adds 2 times of amount alcohol reflux 1 hour again, filters, and reclaims ethanol, and 60 ℃ of dryings promptly get pure product icariine selenium;
(2) lycium barbarum polysaccharide extraction process:
Getting former medicinal 1% zinc sulfate, 45% ethanol of Fructus Lycii moistening 4 hours, is solvent dipping 24 hours with 1% zinc sulfate, 45% ethanol, with the speed seepage of per minute 50ml/5kg, when making transudate reach 7 times of amounts of crude drug, promptly stop seepage, collection is got sepage, reclaim ethanol, and thickening filtration; Be adjusted to every milliliter and contain total amount 150mg, add 0.25% benzoic acid again, 0.1% nipalgin is anticorrosion, leaves standstill 8-10 days, gets the supernatant and filters, and, is dried into dry extract that is;
(3) zinc glycyrrhizate extraction process:
(1) thick zinc glycyrrhetate extraction process: thick Radix Glycyrrhizae powder is measured 0.5% zinc carbonate (ZnCO with 12 times
3) soak seepage after 12 hours, continuous 3 times, effusion is concentrated into original volume 1/5 and filters, and filtrate adds concentrated sulphuric acid to PH3, filters the precipitation of separating out, and washes 3 times, and is dry below 60 ℃, promptly gets thick zinc glycyrrhetate;
(2) pure product zinc glycyrrhizate extraction process: above gained zinc glycyrrhizate 400g adds 4 times of amount ethanol, reflux 3 hours, filter, 2 times of amounts of filtering residue alcohol heating reflux 2 times merges all backflow and adds the 10g active carbon, reflux decolour cooling half an hour, filter, filtrate ammonification controlled atmosphere is to PH7-7.5, till no solid is separated out, the filtrate precipitation, with the small amount of ethanol washing, drying at room temperature gets zinc glycyrrhetate 250g;
(4) matrine selenium extraction process:
(1) extraction process: Herba Sophorae alopecuroidis or root pulverize to coarse powder, add 5 times of amounts of 1% manganese sulfate, 75% ethanol reflux inclined and supernatant in 1 hour, the same method of medicinal residues adds 70% ethanol, 1% manganese sulfate heating and refluxing extraction 2 times again, merge 3 times extracting solution, quiet amount 1-2 days, filter, filtrate recycling ethanol, to do not have the alcohol flavor, be condensed into cream, the oven dry back becomes dry extract standby;
(5) Cornu Cervi Pantotrichum extraction process:
Get Cornu Cervi Pantotrichum, be sawn into the 3-4mm segment, steamed about 20 minutes, peel off fur, be split into 4-5 millimeters thick fritter; Fine and soft and 5 times of amounts of 5% ethanol add in the backflow extractor together, and water-bath refluxed 4 hours, collect backflow, and 2-3 room filtered through gauze is collected filtrate, place below 10 ℃ more than 24 hours, and fine and soft slag is used with method extraction 5 times, and merging filtrate is condensed into cream, is dried into cream for 50 ℃;
(6) astragalin extraction process:
Radix Astragali decoction pieces, decocting boils secondary, each 1.5 hours, extract water liquid concentrating under reduced pressure, concentrated solution adds 95% ethanol makes determining alcohol reach 60%, the leaching precipitate, be dissolved in water, the centrifugal insoluble matter of removing, filtrate decompression is concentrated into stock solution 1/5, add 95% ethanol again and make determining alcohol reach 80%, stand at low temperature is spent the night, and inclining supernatant, heavy filter part adds high concentration ethanol and stirs, and filters washing with alcohol, vacuum drying gets thick astragalus polysaccharides; Again water-soluble, to filter, filtrate is by the DEAE anion-exchange column, washing is collected, astragalin stream part of a speckle of electrophoretic examinations, merge and concentrate, with the reverse flowing water dialysis of dialyzer 24 hours, to take out and remove by filter a small amount of precipitate, filtrate decompression concentrates, add 95% ethanol precipitation, filter, use absolute ethanol washing, promptly in five oxidations, two carbon vacuum dryings;
(7) TANSHINONES extraction and separation process:
Get the Radix Salviae Miltiorrhizae crude drug powder, 95% ethanol heat is carried, and pure extractum refluxes with an amount of benzene, and benzene liquid is mixed on aluminium oxide after concentrating, and carries out alumina column chromatography, and with the benzene flushing, the benzene eluent is concentrated slightly, separates out column crystallization, is TANSHINONES;
(8) albiflorin extracts:
Radix Paeoniae Alba is given birth to coarse powder, presses percolation, with 30% ethanol moistening 4 hours, with 30% alcohol is solvent dipping after 48 hours, with the speed percolation of per minute 50ml/50kg, when the transudate of waiting reaches 5 times of crude drugs, both stopped percolation, collect sepage, steam back alcohol, and add concentrated, filter, after getting a little orange clear filtrate mensuration content, be adjusted to every milliliter and contain total extract 100mg, add 0.2% benzene again; Formic acid is anticorrosion to leave standstill 48 hours, got supernatant liquid filtering, concentrated, and oven dry promptly;
(9) Radix Angelicae Sinensis extraction process:
The Radix Angelicae Sinensis fragment decocts with water three times, merges decoction liquor, evaporation and concentration liquid adds 95% ethanol makes pure content reach 65%, refrigerator was placed 24 hours, and filtered and recycled ethanol is concentrated into 1: 2, adding 95% ethanol again makes and contains alcohol amount and reach 80%, refrigerator was placed 24 hours, and filtered and recycled ethanol adds ammonia and transfers pH value to 8-8.5, placed refrigerator interior 24 hours, it is standby that filtering ammonia is condensed into cream excessively;
(10) preparation process:
Icariine selenium: 40mg/kg/ day, lycium barbarum polysaccharide: 2mg/kg/ day; Pilose antler polysaccharides: 80mg/kg/ day; Albiflorin: 20mg/kg/ day; Astragalin: 26mg/kg/ day; Eumenol: 26mg/kg/ day; TANSHINONES: 26mg/kg/ day; Matrine selenium: 8mg/kg/ day; Zinc glycyrrhizate: 18mg/kg/ day; Vitamin E: 4mg/kg/ day; Vitamin D: 1mg/kg/ day; Amount to 251mg/kg/ day, be divided into 3 packed, every bag of 83.67mg.
Embodiment two:
Other processing step is identical with embodiment one, difference for the Cornu Cervi Pantotrichum extraction process is: remove the skin Cornu Cervi Pantotrichum and be cut into small pieces, with 50% alcohol reflux and filtering and concentrating liquid with Different concentrations of alcohol deproteinization 5 times, filtrate being diluted to contains 60% of crude drug in whole, sterilization, freezing, filter, add repeatedly the activated carbon filtration heating, freezing, concentrate and be dried into cream.
Preparation process is: icariine selenium: 60mg/kg/ day, lycium barbarum polysaccharide: 4mg/kg/ day; Pilose antler polysaccharides: 100mg/kg/ day; Albiflorin: 35mg/kg/ day; Astragalin: 32mg/kg/ day; Eumenol: 32mg/kg/ day; TANSHINONES: 32mg/kg/ day; Matrine selenium: 12mg/kg/ day; Zinc glycyrrhizate: 22mg/kg/ day; Vitamin E: 6mg/kg/ day; Vitamin D: 2mg/kg/ day; Amount to 337mg/kg/ day, be divided into 3 packed, every bag of 112.33mg.
Embodiment three:
Other processing step is identical with embodiment one, and difference for the astragalin extraction process is: get Radix Astragali 10kg and add injection water and soak half an hour, decoct each 1 hour 3 times, filter, merging filtrate is concentrated into 5000ml, add alcohol and reach 60%, stir, placed 40 hours to containing the alcohol amount, filter, reclaim concentration of alcohol to 2000ml, adding concentration of alcohol again is more than 80%, placed 40 hours, and filtered, reclaim ethanol, being concentrated into does not have the alcohol flavor, is condensed into cream, dry for standby.
Preparation process:
Icariine selenium: 50mg/kg/ day, lycium barbarum polysaccharide: 3mg/kg/ day; Pilose antler polysaccharides: 90mg/kg/ day; Albiflorin: 30mg/kg/ day; Astragalin: 30mg/kg/ day; Eumenol: 30mg/kg/ day; TANSHINONES: 30mg/kg/ day; Matrine selenium: 10mg/kg/ day; Zinc glycyrrhizate: 20mg/kg/ day; Vitamin E: 5mg/kg/ day; Vitamin D: 1.5mg/kg/ day; Amount to 329.5mg/kg/ day, be divided into 3 packed, every bag of 109.8mg.
Embodiment four:
Other processing step is identical with embodiment one, difference for the TANSHINONES extraction process is: round reason and clean Radix Salviae Miltiorrhizae, with decocting in water three times, filter, merge three times decocting liquid, be concentrated into every milliliter and contain crude drug 1.5-2g, add 95% ethanol and make medicinal liquid contain alcohol amount to reach 85%, place filtration in 4 hours, concentrate to reclaim ethanol and be concentrated into every milliliter and contain raw material 3-5g, add 95% ethanol again and make medicinal liquid contain crude drug to reach 85%, placed 4 hours, filtrate recycling ethanol is concentrated into every milliliter and contains crude drug 3-5g, pour 13 times of amount distilled water, place filtration in 24 hours, filtrate is concentrated into every milliliter and contains crude drug 3-5g, reuse 95% ethanol precipitation once, make medicinal liquid contain the alcohol amount and reach 80%, place filtration in 24 hours, filtrate recycling ethanol is to there not being the alcohol flavor, dense one-tenth cream is dried into powder.
Preparation process is:
Icariine selenium: 55mg/kg/ day, lycium barbarum polysaccharide: 4mg/kg/ day; Pilose antler polysaccharides: 95mg/kg/ day; Albiflorin: 22mg/kg/ day; Astragalin: 27mg/kg/ day; Eumenol: 28mg/kg/ day; TANSHINONES: 27mg/kg/ day; Matrine selenium: 9mg/kg/ day; Zinc glycyrrhizate: 19mg/kg/ day; Vitamin E: 5mg/kg/ day; Vitamin D: 1mg/kg/ day; Amount to 292mg/kg/ day, be divided into 3 packed, every bag of 97.33mg.
Claims (5)
1. the medicine of an invigorating kidney, promoting blood circulation and promoting skeleton formation is characterised in that it consists of icariine selenium: 40-60mg/kg, lycium barbarum polysaccharide: 2-4mg/kg; Pilose antler polysaccharides: 80-100mg/kg; Albiflorin: 20-35mg/kg; Astragalin: 26-32mg/kg; Eumenol: 26-32mg/kg; TANSHINONES: 26-32mg/kg; Matrine selenium: 8-12mg/kg; Zinc glycyrrhizate: 18-22mg/kg; Vitamin E: 4-6mg/kg; Vitamin D: 1-2mg/kg; Add up to 251-337mg/kg.
2. the preparation method of the medicine of an invigorating kidney, promoting blood circulation and promoting skeleton formation is characterized in that concrete preparation technology is as follows:
(1) icariine selenium extraction process:
(1) crude product icariine selenium extraction process: the former medicine of rough lumber adds 8 times of amount 1% selenic acid liquid, kept 80 ℃ of reflux, extract, 3 hours, filtering the extraction medicinal liquid puts in addition, residue adds 5 times of amount 1% selenic acid liquid again, pressing the Same Way operation extracted 2 hours, medicinal liquid is extracted in multiple filter and preceding liquid merges, and gathers all selenic acid extracting solution, is condensed into than the 1.1-1.2 that weighs, add 95% ethanol equivalent precipitated impurities, left standstill 12-14 hour, and extracted supernatant liquid filtering, precipitation is with 45% washing with alcohol 2-3 time, the solubility composition washes out in will precipitating, filtrate and washing liquid merge, reclaim ethanol, concentrating under reduced pressure is cooled to cream, promptly gets the thick extracted extract of Herba Epimedii selenium;
(2) pure product icariine selenium extraction process: the thick extracted extract of Herba Epimedii selenium adds 70%4 times of amount ethanol, and heating and refluxing extraction 2 hours is filtered, and filtering residue adds 2 times of amount alcohol reflux 1 hour again, filters, and reclaims ethanol, and 60 ℃ of dryings promptly get pure product icariine selenium;
(2) lycium barbarum polysaccharide extraction process:
Getting former medicinal 1% zinc sulfate, 45% ethanol of Fructus Lycii moistening 4 hours, is solvent dipping 24 hours with 1% zinc sulfate, 45% ethanol, with the speed seepage of per minute 50ml/5kg, when making transudate reach 7 times of amounts of crude drug, promptly stop seepage, collection is got sepage, reclaim ethanol, and thickening filtration; Be adjusted to every milliliter and contain total amount 150mg, add 0.25% benzoic acid again, 0.1% nipalgin is anticorrosion, leaves standstill 8-10 days, gets the supernatant and filters, and, is dried into dry extract that is;
(3) zinc glycyrrhizate extraction process:
(1) thick zinc glycyrrhetate extraction process: thick Radix Glycyrrhizae powder is measured 0.5% zinc carbonate (ZnCO with 12 times
3) soak seepage after 12 hours, continuous 3 times, effusion is concentrated into original volume 1/5 and filters, and filtrate adds concentrated sulphuric acid to PH3, filters the precipitation of separating out, and washes 3 times, and is dry below 60 ℃, promptly gets thick zinc glycyrrhetate;
(2) pure product zinc glycyrrhizate extraction process: above gained zinc glycyrrhizate 400g adds 4 times of amount ethanol, reflux 3 hours, filter, 2 times of amounts of filtering residue alcohol heating reflux 2 times merges all backflow and adds the 10g active carbon, reflux decolour cooling half an hour, filter, filtrate ammonification controlled atmosphere is to PH7-7.5, till no solid is separated out, the filtrate precipitation, with the small amount of ethanol washing, drying at room temperature gets zinc glycyrrhetate 250g;
(4) matrine selenium extraction process:
(1) extraction process: Herba Sophorae alopecuroidis or root pulverize to coarse powder, add 5 times of amounts of 1% manganese sulfate, 75% ethanol reflux inclined and supernatant in 1 hour, the same method of medicinal residues adds 70% ethanol, 1% manganese sulfate heating and refluxing extraction 2 times again, merge 3 times extracting solution, quiet amount 1-2 days, filter, filtrate recycling ethanol, to do not have the alcohol flavor, be condensed into cream, the oven dry back becomes dry extract standby;
(5) Cornu Cervi Pantotrichum extraction process:
Get Cornu Cervi Pantotrichum, be sawn into the 3-4mm segment, steamed about 20 minutes, peel off fur, be split into 4-5 millimeters thick fritter; Fine and soft and 5 times of amounts of 5% ethanol add in the backflow extractor together, and water-bath refluxed 4 hours, collect backflow, and 2-3 room filtered through gauze is collected filtrate, place below 10 ℃ more than 24 hours, and fine and soft slag is used with method extraction 5 times, and merging filtrate is condensed into cream, is dried into cream for 50 ℃;
(6) astragalin extraction process:
Radix Astragali decoction pieces, decocting boils secondary, each 1.5 hours, extract water liquid concentrating under reduced pressure, concentrated solution adds 95% ethanol makes determining alcohol reach 60%, the leaching precipitate, be dissolved in water, the centrifugal insoluble matter of removing, filtrate decompression is concentrated into stock solution 1/5, add 95% ethanol again and make determining alcohol reach 80%, stand at low temperature is spent the night, and inclining supernatant, heavy filter part adds high concentration ethanol and stirs, and filters washing with alcohol, vacuum drying gets thick astragalus polysaccharides; Again water-soluble, to filter, filtrate is by the DEAE anion-exchange column, washing is collected, astragalin stream part of a speckle of electrophoretic examinations, merge and concentrate, with the reverse flowing water dialysis of dialyzer 24 hours, to take out and remove by filter a small amount of precipitate, filtrate decompression concentrates, add 95% ethanol precipitation, filter, use absolute ethanol washing, promptly in five oxidations, two carbon vacuum dryings;
(7) TANSHINONES extraction and separation process:
Get the Radix Salviae Miltiorrhizae crude drug powder, 95% ethanol heat is carried, and pure extractum refluxes with an amount of benzene, and benzene liquid is mixed on aluminium oxide after concentrating, and carries out alumina column chromatography, and with the benzene flushing, the benzene eluent is concentrated slightly, separates out column crystallization, is TANSHINONES;
(8) albiflorin extracts:
Radix Paeoniae Alba is given birth to coarse powder, presses percolation, with 30% ethanol moistening 4 hours, with 30% alcohol is solvent dipping after 48 hours, with the speed percolation of per minute 50ml/50kg, when the transudate of waiting reaches 5 times of crude drugs, both stopped percolation, collect sepage, steam back alcohol, and add concentrated, filter, after getting a little orange clear filtrate mensuration content, be adjusted to every milliliter and contain total extract 100mg, add 0.2% benzene again; Formic acid is anticorrosion to leave standstill 48 hours, got supernatant liquid filtering, concentrated, and oven dry promptly;
(9) Radix Angelicae Sinensis extraction process:
The Radix Angelicae Sinensis fragment decocts with water three times, merges decoction liquor, evaporation and concentration liquid adds 95% ethanol makes pure content reach 65%, refrigerator was placed 24 hours, and filtered and recycled ethanol is concentrated into 1: 2, adding 95% ethanol again makes and contains alcohol amount and reach 80%, refrigerator was placed 24 hours, and filtered and recycled ethanol adds ammonia and transfers pH value to 8-8.5, placed refrigerator interior 24 hours, it is standby that filtering ammonia is condensed into cream excessively;
(10) preparation process:
Proportioning is counted icariine selenium with amount: 40-60mg/kg/ day, lycium barbarum polysaccharide: 2-4mg/kg/ day; Pilose antler polysaccharides: 80-100mg/kg/ day; Albiflorin: 20-35mg/kg/ day; Astragalin: 26-32mg/kg/ day; Eumenol: 26-32mg/kg/ day; TANSHINONES: 26-32mg/kg/ day; Matrine selenium: 8-12mg/kg/ day; Zinc glycyrrhizate: 18-22mg/kg/ day; Vitamin E: 4-6mg/kg/ day; Vitamin D: 1-2mg/kg/ day; Amount to 251-337mg/kg/ day, be divided into 3 packed, every bag of 83.67-112.33mg.
3. the preparation method of the medicine of invigorating kidney, promoting blood circulation and promoting skeleton formation as claimed in claim 2, it is characterized in that the Cornu Cervi Pantotrichum extraction process is: remove the skin Cornu Cervi Pantotrichum and be cut into small pieces, with 50% alcohol reflux and filtering and concentrating liquid with Different concentrations of alcohol deproteinization 5 times, filtrate being diluted to contains 60% of crude drug in whole, sterilization, freezing, filter, add repeatedly the activated carbon filtration heating, freezing, concentrate and be dried into cream.
4. the preparation method of the medicine of invigorating kidney, promoting blood circulation and promoting skeleton formation as claimed in claim 2 is characterized in that the astragalin extraction process is: get Radix Astragali 10kg and add injection water and soak half an hour, decoct each 1 hour 3 times, filter, merging filtrate is concentrated into 5000ml, add alcohol and reach 60%, stir, placed 40 hours to containing the alcohol amount, filter, reclaim concentration of alcohol to 2000ml, adding concentration of alcohol again is more than 80%, placed 40 hours, and filtered, reclaim ethanol, being concentrated into does not have the alcohol flavor, is condensed into cream, dry for standby.
5. the preparation method of the medicine of invigorating kidney, promoting blood circulation and promoting skeleton formation as claimed in claim 2, it is characterized in that the TANSHINONES extraction process is: round reason and clean Radix Salviae Miltiorrhizae, with decocting in water three times, filter, merge three times decocting liquid, be concentrated into every milliliter and contain crude drug 1.5-2g, add 95% ethanol and make medicinal liquid contain alcohol amount to reach 85%, place filtration in 4 hours, concentrate to reclaim ethanol and be concentrated into every milliliter and contain raw material 3-5g, add 95% ethanol again and make medicinal liquid contain crude drug to reach 85%, placed 4 hours, filtrate recycling ethanol is concentrated into every milliliter and contains crude drug 3-5g, pour 13 times of amount distilled water, place filtration in 24 hours, filtrate is concentrated into every milliliter and contains crude drug 3-5g, reuse 95% ethanol precipitation once, make medicinal liquid contain the alcohol amount and reach 80%, place filtration in 24 hours, filtrate recycling ethanol is to there not being the alcohol flavor, dense one-tenth cream is dried into powder.
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| CN101700119B (en) * | 2009-06-12 | 2013-05-08 | 李文金 | Oral agent for improving sub-health symptoms in adults |
| CN104397529A (en) * | 2014-12-04 | 2015-03-11 | 李志成 | Sea cucumber, cistanche and pilose antler polysaccharide and preparation technology thereof |
| CN105055791A (en) * | 2015-08-09 | 2015-11-18 | 辛海军 | Medicine used for treating lumbar disc herniation, and preparation method thereof |
| CN105381326A (en) * | 2015-11-18 | 2016-03-09 | 王海鹏 | Compound cotinus coggygria zinc oral solution and preparation method thereof |
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