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CN121203820A - Preservation method of nitrifying bacteria for aquarium - Google Patents

Preservation method of nitrifying bacteria for aquarium

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Publication number
CN121203820A
CN121203820A CN202511010005.7A CN202511010005A CN121203820A CN 121203820 A CN121203820 A CN 121203820A CN 202511010005 A CN202511010005 A CN 202511010005A CN 121203820 A CN121203820 A CN 121203820A
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CN
China
Prior art keywords
nitrifying bacteria
porous filter
preserving
filter material
sealed
Prior art date
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Pending
Application number
CN202511010005.7A
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Chinese (zh)
Inventor
门美丽
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Individual
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Individual
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Publication date
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Priority to CN202511010005.7A priority Critical patent/CN121203820A/en
Publication of CN121203820A publication Critical patent/CN121203820A/en
Pending legal-status Critical Current

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Abstract

The invention relates to the technical field of aquarium aquariums, in particular to a method for preserving nitrifying bacteria for an aquarium. According to the invention, nitrifying bacteria are combined with the porous filter material and stored in a specific preservation solution, so that a proper living environment is provided for nitrifying bacteria, and stable metabolic activity can be kept during storage, thereby prolonging the storage time of nitrifying bacteria. The invention can provide a large amount of nitrifying bacteria with biological activity, and a sufficient and stable nitrifying bacteria system can be quickly formed without culturing after the nitrifying bacteria are added into a fish tank. The invention has simple operation, low cost and convenient popularization and application, can effectively solve the problem of slow nitrifying bacteria culture in the aquatic animal culture, effectively improves the water quality of the aquarium, and brings convenience for aquatic animal culture lovers.

Description

Preservation method of nitrifying bacteria for aquarium
Technical Field
The invention relates to the technical field of aquarium aquariums, in particular to a method for preserving nitrifying bacteria for an aquarium.
Background
In the field of aquatic farming, nitrifying bacteria are key microorganisms for maintaining water quality balance, and can convert ammonia and nitrite into nitrate, so that the content of harmful substances in water is reduced, and a healthy living environment is provided for aquatic organisms such as fishes. However, nitrifying bacteria are difficult to preserve in the external environment and are easily inactivated by the influence of factors such as temperature, humidity, illumination and the like, so that the effect is greatly reduced when the nitrifying bacteria are used.
In the prior art, articles for culturing nitrifying bacteria such as bacitrate and the like to improve the nitrifying bacteria content in the fish tank exist, but the nitrifying bacteria does not contain abundant nitrifying bacteria, and when the nitrifying bacteria are placed in the fish tank, a nitrifying bacteria system can be formed after about 3-7 days of culture. Therefore, the prior art is difficult to quickly increase the content of nitrifying bacteria in the fish tank and quickly and stably construct a nitrifying bacteria system in the fish tank.
Disclosure of Invention
The invention aims to provide a preservation method of nitrifying bacteria for a fish tank aquarium, which can effectively prolong the preservation time of nitrifying bacteria, keep the activity and stability of nitrifying bacteria, ensure that the nitrifying bacteria can rapidly play a role in use and improve the purification efficiency of water quality in the aquarium.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
a preservation method of nitrifying bacteria for a fish tank aquarium comprises the following steps:
s1, putting cultivated nitrifying bacteria into preservation solution, and then adding a dry porous filter material into the preservation solution;
S2, placing preservation solution containing nitrifying bacteria and a porous filter material into a closed container for preservation, wherein the preservation temperature is controlled between 15 ℃ and 25 ℃;
the preservation solution contains a carbon source, a nitrogen source, a phosphorus source and microelements so as to support basic metabolism of nitrifying bacteria;
the volume of the preservation solution is larger than that of the porous filter material, so that the porous filter material can be fully soaked in the preservation solution.
As an improvement, the porous filter material can be an artificial filter material or a natural filter material, and comprises one or a combination of a plurality of ceramic rings, vesuvianite, biological balls or activated carbon.
As a modification, the carbon source is one or more of glucose, sucrose or sodium lactate.
As an improvement, the nitrogen source is one or more of potassium nitrate, ammonium sulfate or urea.
As an improvement, the phosphorus source is potassium dihydrogen phosphate or disodium hydrogen phosphate.
As an improvement, the trace elements include iron, manganese, zinc, copper, molybdenum, and cobalt.
As an improvement, the preservation solution is sterilized before use, and the PH value is between 6.5 and 8.0.
As an improvement, the nitrifying bacteria are a mixed flora of nitrifying monads and nitrifying bacilli.
As an improvement, the closed container is a sealed bottle, a sealed tank or a barreled sealed container, and a sealing cover is arranged at the top of the sealed bottle or the sealed tank;
The porous filter material is placed in a sealing bottle or a sealing tank, and the preservation solution containing nitrifying bacteria is completely immersed in the porous filter material and then sealed by a sealing cover.
As an improvement, the closed container is a sealed bag;
The porous filter material is arranged in the sealing belt, and after the preservation solution containing nitrifying bacteria is added into the sealing bag, the inside of the porous filter material is fully soaked, and the surface of the porous filter material is soaked.
The invention has the advantages that:
According to the invention, nitrifying bacteria are combined with the porous filter material and stored in a specific preservation solution, so that a proper living environment is provided for nitrifying bacteria, and stable metabolic activity can be kept during storage, thereby prolonging the storage time of nitrifying bacteria. The invention can provide a large amount of nitrifying bacteria with biological activity, and a sufficient and stable nitrifying bacteria system can be quickly formed without culturing after the nitrifying bacteria are added into a fish tank.
The invention has simple operation, low cost and convenient popularization and application, can effectively solve the problem of slow nitrifying bacteria culture in the aquatic animal culture, effectively improves the water quality of the aquarium, and brings convenience for aquatic animal culture lovers.
Drawings
FIG. 1 is a block diagram showing a sealed tank in a method for preserving nitrifying bacteria for a aquarium in example 1.
FIG. 2 is a view showing the structure of a sealed bag in a method for preserving nitrifying bacteria for a aquarium in example 2.
FIG. 3 is a view showing the constitution of a sealed container for a tank in a method for preserving nitrifying bacteria for a aquarium in example 3.
The marks in the figure:
1-sealed pot, 2-sealed cover, 3-biological ball, 4-sealed bag, 5-vesuvianite, 6-barren sealed container.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Example 1
The embodiment discloses a preservation method of nitrifying bacteria for a fish tank aquarium, which comprises the following steps:
s1, putting cultivated nitrifying bacteria into preservation solution, and then adding a dry porous filter material into the preservation solution;
S2, placing preservation solution containing nitrifying bacteria and a porous filter material into a closed container for preservation, wherein the preservation temperature is controlled between 15 ℃ and 25 ℃;
in this example, the preservation solution contains a carbon source, a nitrogen source, a phosphorus source and trace elements to support basic metabolism of nitrifying bacteria.
In this example, the pH of the preservation solution was between 6.5 and 8.0.
The nitrifying bacteria are mixed flora of nitrifying monad and nitrifying bacillus.
The components in the preservation solution are as follows:
TABLE 1 Components in preservation solution
In this embodiment, the volume of the preservation solution is larger than the volume of the porous filter medium, so that the porous filter medium can be fully immersed in the preservation solution. The porous filter material is a bio-sphere.
Fig. 1 shows a closed container of the present embodiment, which comprises a sealed can 1 and a sealing cover 2. The biological ball 3 is arranged in the sealed tank 1, and after the preservation solution is added into the sealed tank 1, the biological ball 3 is completely soaked, so that the biological ball 3 is always in the preservation solution.
The biological ball is made of porous plastic or ceramic, and micropores are distributed on the surface and inside of the biological ball, so that a large number of attachment sites can be provided for nitrifying bacteria (nitrifying monads and nitrifying bacilli) to form a stable biological film. When the biological ball is completely immersed by the preservation solution, the pores of the biological ball can continuously absorb the nutrient solution, and even if the sealing tank is inclined or swayed, the biological ball can still maintain the wet environment of the flora, so as to ensure the activity of the flora. When in use, the biological balls and the preservation solution are directly placed into the fish tank, and the attached nitrifying bacteria group does not need to be additionally activated, so that the establishment period of the nitrifying bacteria group system can be effectively shortened.
Example 2
The embodiment discloses a preservation method of nitrifying bacteria for a fish tank aquarium, which comprises the following steps:
s1, putting cultivated nitrifying bacteria into preservation solution, and then adding a dry porous filter material into the preservation solution;
S2, placing preservation solution containing nitrifying bacteria and a porous filter material into a closed container for preservation, wherein the preservation temperature is controlled between 15 ℃ and 25 ℃;
in this example, the preservation solution contains a carbon source, a nitrogen source, a phosphorus source and trace elements to support basic metabolism of nitrifying bacteria.
In this example, the pH of the preservation solution was between 6.5 and 8.0.
The nitrifying bacteria are mixed flora of nitrifying monad and nitrifying bacillus.
The preservation solution of this example comprises the following components:
TABLE 2 Components in preservation solution
In this embodiment, the volume of the preservation solution is larger than the volume of the porous filter medium, so that the porous filter medium can be fully immersed in the preservation solution. The porous filter material is volcanic stone.
As shown in fig. 2, the sealed container in this embodiment is a sealed bag 4, and the vesuvianite 5 is placed inside the sealed bag 4, and after the preservation solution containing nitrifying bacteria is added into the sealed bag 4, the inside of the vesuvianite 5 is fully soaked, and the inside and the surface of the vesuvianite 5 are soaked.
The ceramic ring and the activated carbon can be used as the porous filter materials or the four porous filter materials can be combined to achieve the effects of synergistic combination and synergistic effect, and the effects of bacterial colony attachment, water quality purification and long-term stability are considered.
Example 3
The embodiment discloses a preservation method and a preservation container for nitrifying bacteria for a fish tank aquarium.
The preservation method of this example is the same as that of example 1.
As shown in fig. 3, this embodiment employs a sealed container 6 in a barrel. The biological ball 3 is arranged in the barreled sealed container 6, and after the preservation liquid is added into the barreled sealed container 6, the biological ball 3 is completely soaked, so that the biological ball 3 is always in the preservation liquid.
The barreled sealed container 6 in this embodiment adopts a 2L container, which can hold more nitrifying bacteria. When the user's aquarium is large, the barreled sealed container 6 of the present embodiment may be selected. If the user's aquarium or aquarium is medium, 500-1000mL of the sealed tank 1 as described in example 1 may be selected. If the user's aquarium is small, a 200-400mL sealed pouch 4 as described in example 2 may be selected.
The present invention is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present invention and the inventive concept thereof, can be replaced or changed within the scope of the present invention.

Claims (10)

1.一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,包括如下步骤:1. A method for preserving nitrifying bacteria for aquariums, characterized by comprising the following steps: S1.将培育好的硝化细菌投入保存液中,然后向保存液中加入干燥的多孔滤材;S1. Add the cultured nitrifying bacteria into the preservation solution, and then add dry porous filter media to the preservation solution; S2.将含有硝化细菌的保存液及多孔滤材置于密闭容器中保存,保存温度控制在15-25℃之间;S2. Store the preservation solution containing nitrifying bacteria and porous filter media in a sealed container, and control the storage temperature between 15-25℃. 所述保存液内含有碳源、氮源、磷源及微量元素,以支持硝化细菌的基本代谢;The preservation solution contains carbon, nitrogen, phosphorus and trace elements to support the basic metabolism of nitrifying bacteria. 所述保存液的体积大于多孔滤材的体积,使多孔滤材可以充分浸润在保存液内。The volume of the preservation liquid is larger than the volume of the porous filter material, so that the porous filter material can be fully immersed in the preservation liquid. 2.根据权利要求1所述的一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,所述多孔滤材为陶瓷环、火山石、生物球或活性炭中的一种或多种的组合。2. The method for preserving nitrifying bacteria for aquariums according to claim 1, characterized in that the porous filter material is one or more of ceramic rings, volcanic rock, bio-balls, or activated carbon. 3.根据权利要求1所述的一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,所述碳源为葡萄糖、蔗糖或乳酸钠中的一种或多种。3. The method for preserving nitrifying bacteria for aquariums according to claim 1, wherein the carbon source is one or more of glucose, sucrose, or sodium lactate. 4.根据权利要求1所述的一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,所述氮源为硝酸钾、硫酸铵或尿素中的一种或多种。4. The method for preserving nitrifying bacteria for aquariums according to claim 1, wherein the nitrogen source is one or more of potassium nitrate, ammonium sulfate, or urea. 5.根据权利要求1所述的一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,所述磷源为磷酸二氢钾或磷酸氢二钠。5. The method for preserving nitrifying bacteria for aquariums according to claim 1, wherein the phosphorus source is potassium dihydrogen phosphate or disodium hydrogen phosphate. 6.根据权利要求1所述的一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,所述微量元素包括铁、锰、锌、铜、钼和钴。6. The method for preserving nitrifying bacteria for aquariums according to claim 1, wherein the trace elements include iron, manganese, zinc, copper, molybdenum and cobalt. 7.根据权利要求1所述的一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,所述保存液在使用前经过灭菌处理,且PH值为6.5-8.0之间。7. The method for preserving nitrifying bacteria for aquariums according to claim 1, characterized in that the preservation solution is sterilized before use and has a pH value between 6.5 and 8.0. 8.根据权利要求1所述的一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,所述硝化细菌为亚硝化单胞菌和硝化杆菌的混合菌群。8. A method for preserving nitrifying bacteria for aquariums according to claim 1, wherein the nitrifying bacteria are a mixed flora of Nitrosomonas and Nitrobacterium. 9.根据权利要求1所述的一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,所述密闭容器为密封瓶、密封罐或桶装密封容器,所述密封瓶或密封罐顶部设有密封盖;9. A method for preserving nitrifying bacteria for aquariums according to claim 1, characterized in that the sealed container is a sealed bottle, sealed jar, or barrel-type sealed container, and the sealed bottle or sealed jar is provided with a sealing cap on the top; 所述多孔滤材置于密封瓶或密封罐内,含有硝化细菌的所述保存液完全浸没多孔滤材后,被密封盖密封。The porous filter material is placed in a sealed bottle or sealed container, and after the preservation solution containing nitrifying bacteria completely submerges the porous filter material, it is sealed with a sealing cap. 10.根据权利要求1所述的一种鱼缸水族缸用硝化细菌的保存方法,其特征在于,所述密闭容器为密封袋;10. A method for preserving nitrifying bacteria for aquariums according to claim 1, wherein the sealed container is a sealed bag; 所述多孔滤材置于密封带内部,含有硝化细菌的所述保存液加入密封袋后,充分浸润多孔滤材内部并使多孔滤材表面浸湿。The porous filter material is placed inside the sealing strip. After the preservation solution containing nitrifying bacteria is added to the sealing bag, it fully wets the interior of the porous filter material and wets the surface of the porous filter material.
CN202511010005.7A 2025-07-22 2025-07-22 Preservation method of nitrifying bacteria for aquarium Pending CN121203820A (en)

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CN202511010005.7A CN121203820A (en) 2025-07-22 2025-07-22 Preservation method of nitrifying bacteria for aquarium

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CN121203820A true CN121203820A (en) 2025-12-26

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