CN120800886B - A portable wetland plant seed bank stratified collection and cultivation device - Google Patents
A portable wetland plant seed bank stratified collection and cultivation deviceInfo
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- CN120800886B CN120800886B CN202511294898.2A CN202511294898A CN120800886B CN 120800886 B CN120800886 B CN 120800886B CN 202511294898 A CN202511294898 A CN 202511294898A CN 120800886 B CN120800886 B CN 120800886B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0293—Seed or shoot receptacles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application relates to a portable wetland plant seed warehouse layered collection and culture device, which relates to the technical field of plant seed sampling. The lower pressure rod can be driven downwards through the driving mechanism to drive the coaxially connected culture tube to enter soil, and a culture cavity is arranged in the culture tube for collecting mud column samples. A plurality of mud column dividing mechanisms in switchable states are arranged in the wall of the culture tube, and radially extend into the culture cavity after sampling is completed to divide the mud column into spaces. The anti-falling mechanism is arranged at the inlet of the culture cavity, can close the inlet after sampling is completed and prevents the mud column from sliding off. The device compact structure, portable is applicable to and carries out stable collection and interim layering culture to plant seed storehouse sample at the wetland of complicated topography, and the sample is cut apart clearly, is preserved completely, and the sample is more convenient with the culture simultaneously.
Description
Technical Field
The application relates to the technical field of plant seed cultivation, in particular to a portable wetland plant seed warehouse layered collection and cultivation device.
Background
At present, under the background of climate change, gate dam construction and human activity superposition interference, wetland vegetation is seriously degraded, and the stable exertion of key functions of a wetland ecological system faces serious risks, so scientific investigation and in-situ/ex-situ recovery taking a wetland plant seed bank as objects are regarded as important paths for maintaining the ecological safety of the kidney of the earth.
However, the applicant finds that in long-term field monitoring, sampling and culturing practices, the existing wetland plant seed warehouse collecting device is heavy and has no culturing function, and mainly relies on manpower to drill and pull out the soil seed warehouse in a sampling frame after qualitative layering split charging for culturing, so that the soil seed warehouse cannot be completely and accurately taken out, the sampling and culturing process is very time-consuming and labor-consuming, and the sampling and culturing efficiency and accuracy are affected.
Disclosure of Invention
The embodiment of the application provides a portable wetland plant seed bank layered collection and culture device, which aims to at least partially solve the technical problems.
In order to achieve the above object, according to the present application, there is provided a portable wetland plant seed bank layered collection and culture device comprising:
A multi-terrain support;
the driving mechanism is detachably arranged on the multi-terrain support frame;
The first end of the lower pressure rod is detachably arranged at the driving end of the driving mechanism, the culture tube is detachably and coaxially connected with the second end of the lower pressure rod, and a culture cavity is coaxially formed in the culture tube;
a plurality of mud column dividing mechanisms are arranged in the wall of the culture tube, the mud column dividing mechanisms are configured to have at least a first state and a second state, the mud column dividing mechanisms are accommodated in the wall of the culture tube when the mud column dividing mechanisms are in the first state, and the mud column dividing mechanisms are radially arranged in the culture cavity when the mud column dividing mechanisms are in the second state so as to divide mud columns in the culture cavity;
the anti-falling mechanism is arranged at the inlet of the culture cavity of the culture tube in an opening and closing mode and is used for opening or closing the inlet of the culture cavity.
Optionally, the mud column dividing mechanism comprises a push-pull component and a bendable dividing component, wherein,
The culture cavity is configured to be axially and eccentrically arranged in the culture tube, so that the culture tube is positioned at two sides of the culture cavity to form a first tube wall and a second tube wall with different tube wall thicknesses, the thickness of the first tube wall is larger than that of the second tube wall, a plurality of mounting grooves are axially formed in the first tube wall, and the push-pull assembly is arranged in the mounting grooves;
The first pipe wall is internally provided with an arc-shaped groove communicated with the mounting groove, the groove width of the arc-shaped groove is smaller than that of the mounting groove, the first end of the arc-shaped groove is communicated with the mounting groove, the second end of the arc-shaped groove is communicated with the culture cavity, the bendable segmentation component is arranged in the mounting groove and the arc-shaped groove, and the push-pull component is used for driving the bendable segmentation component to move in the mounting groove, the arc-shaped groove and the culture cavity;
When the mud column dividing mechanism is in a first state, the bendable dividing assembly is positioned in the mounting groove and the arc groove, and when the mud column dividing mechanism is in a second state, the bendable dividing assembly is positioned in the arc groove and the culture cavity.
Optionally, the push-pull assembly includes first electric jar and push plate, first electric jar demountable installation in the mounting groove, the push plate slidable locates in the mounting groove, just push plate coaxial coupling in on the piston rod of first electric jar, bendable split assembly connect in the one end that first electric jar was kept away from to the push plate.
Optionally, the bendable dividing assembly includes a connecting metal sheet and a bendable piece, wherein a first end of the connecting metal sheet is connected with the pushing plate, and the bendable piece is connected to a second end of the connecting metal sheet;
The arc-shaped groove comprises a vertical groove section, an arc-shaped groove section and a transverse groove section, wherein the vertical groove section is coaxially communicated with the mounting groove, the transverse groove section is radially communicated with the culture cavity, two ends of the arc-shaped groove section are respectively communicated with the vertical groove section and the transverse groove section, the connecting metal sheet is configured to slide in the vertical groove section and the mounting groove, and the bendable piece is configured to slide in the vertical groove section, the arc-shaped groove section, the transverse groove section and the culture cavity.
Optionally, the mud column dividing mechanism further comprises a guide assembly, the guide assembly comprises a first guide plate and a second guide plate, the first guide plate and the second guide plate are both installed on the inner end wall of the installation groove in parallel, the first guide plate and the second guide plate are respectively located at two sides of the vertical groove section, a guide cavity coaxially communicated with the vertical groove section is reserved between the first guide plate and the second guide plate, the connecting metal sheet is configured to be slidably located in the guide cavity, and the thickness of the connecting metal sheet is identical to the width of the vertical groove section and the width of the guide cavity;
Two sliding grooves are formed in the pushing plate, and the two sliding grooves are respectively matched with the first guide plate and the second guide plate in a sliding insertion manner.
Optionally, the bendable piece is a spring steel sheet, one end of the spring steel sheet is welded with the connecting metal sheet, a first guiding groove is annularly arranged on the inner wall of the culture cavity, and the first guiding groove is radially communicated with the transverse groove section and is configured for sliding in a part of the spring steel sheet so as to cut off the culture cavity radially.
Optionally, the device also comprises a limiting mechanism, wherein the limiting mechanism comprises a limiting steel pipe, a rotating column, a limiting block, a rubber ring and a detachable driving piece,
An embedded groove is axially formed in the first pipe wall, the limiting steel pipe is fixedly connected in the embedded groove, the rotating column is rotatably inserted into the limiting steel pipe, the rubber ring is embedded between the rotating column and the limiting steel pipe, and the lengths of the limiting steel pipe and the rotating column are smaller than the depth of the embedded groove;
The limiting device comprises a limiting steel pipe, a first pipe wall, a second pipe wall, a limiting plate, a limiting block, a clamping groove, a rotating column, a limiting block, a limiting plate and a rotary column, wherein the first pipe wall is internally provided with an arc-shaped strip hole, the first pipe wall is internally provided with a through cavity, the through cavity is provided with a through sub cavity and a avoidance sub cavity, the through sub cavity is communicated with the clamping groove and is opposite to the clamping groove when the mud column dividing mechanism is in a first state, the avoidance sub cavity is communicated with one side of the through sub cavity, the side wall of the pushing plate is provided with the clamping groove, the limiting block is connected to the rotating column, and the limiting block is configured to sequentially penetrate through the arc-shaped strip hole and the through sub cavity and then extend into the clamping groove when the mud column dividing mechanism is in the first state so as to limit the axial movement of the pushing plate, and rotate along the extending direction of the arc-shaped strip hole under the rotating column so as to relieve the axial limitation of the pushing plate;
the detachable driving piece comprises a rotating handle and a plug-in rod, the rotating handle is vertically connected with the plug-in rod, the cross section of the plug-in rod is rectangular, a rectangular groove is axially formed in the rotating column, and the plug-in rod is configured to be in plug-in fit with the rectangular groove.
Optionally, the anti-disengaging mechanism comprises a second electric cylinder and a bendable blocking component, the second electric cylinder is detachably installed in the installation groove, an arc bending channel is communicated between the installation groove and the culture cavity, and the bendable blocking component is slidably arranged in the installation groove, the arc bending channel and the culture cavity;
the bendable blocking assembly comprises a plurality of round rods and rubber sealing layers, wherein the round rods are arranged in parallel along the mounting groove, two adjacent round rods are in rolling fit relatively, and the rubber sealing layers are coated on the outer parts of the round rods;
The inner wall of the culture cavity is provided with a second guide groove in a ring way near the inlet of the culture cavity, and the second guide groove is radially communicated with the arc-shaped bending channel and is configured for the part of the bendable blocking component to slide in so as to block the inlet of the culture cavity.
Optionally, the driving mechanism comprises a hydraulic oil cylinder, and a piston rod of the hydraulic oil cylinder is coaxially connected to the end part of the pressure-down rod.
Optionally, the device further comprises a sterile culture bin, wherein the sterile culture bin can be sleeved at the inlet end of the culture tube.
In summary, the present application includes at least one of the following beneficial technical effects:
Through with many topography support frames, actuating mechanism, depression bar, cultivate pipe, mud post cutting mechanism and anti-disengaging mechanism integrated constitution be one set of portable wetland plant seed storehouse layering collection and culture apparatus that can dismantle, have advantages such as structural integration is high, the function subregion is clear and definite, simple operation. The culture tube is stably pressed into soil under different terrain conditions through driving the lower pressure rod by the driving mechanism, the deep mud column sample is obtained, the labor intensity of test personnel is reduced, the culture cavity is arranged inside the culture tube, the structure is compact, and the directional collection and culture of the sample are facilitated. Further, a plurality of mud column dividing mechanisms arranged in the wall of the culture tube can be switched between different working states, so that the mud column dividing mechanisms cannot interfere with the formation of mud columns in a sampling stage, and the mud columns can radially extend into the culture cavity in a dividing stage to divide the space of the mud columns, so that soil samples of different depth levels can be accurately acquired, and culture chambers of different depths can be divided, so that seeds of different heights can be cultured. The anti-disengaging mechanism is arranged at the inlet of the culture cavity, and is used for blocking the inlet when sampling is finished, so that the complete retention of the sample in the process of taking out is facilitated, leakage or external pollution is avoided, and the quality and the effectiveness of sample collection are improved. Therefore, the device is not only suitable for high-efficiency layered sampling and culture of the terrain complex areas such as the wetland, but also has positive effects on sample integrity and sample level distinction, is convenient for subsequent experimental analysis, is convenient for sampling and culture, and simultaneously greatly improves sampling efficiency, culture efficiency and accuracy.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the description of the embodiments will be briefly described below. It is evident that the drawings in the following description are only some embodiments of the application and that other drawings may be obtained from these drawings without inventive effort for a person skilled in the art.
For a more complete understanding of the present application and the advantages thereof, reference is now made to the following descriptions taken in conjunction with the accompanying drawings, in which like reference numerals represent like parts throughout the following description.
FIG. 1 is a schematic view showing a first construction of a culture apparatus according to an embodiment of the application connected to a sterile culture compartment;
FIG. 2 is a schematic view showing a second construction of a culture apparatus according to an embodiment of the application connected to a sterile culture compartment;
FIG. 3 is a schematic view showing the structure of the culture apparatus according to the embodiment of the application with the sterile culture chamber removed;
FIG. 4 is a partial sectional view showing the internal structure of a culture tube according to an embodiment of the present application;
FIG. 5 is an enlarged view of portion A of FIG. 4;
FIG. 6 is an enlarged view of portion B of FIG. 4;
FIG. 7 is a first cross-sectional view schematically showing the internal structure of a culture tube according to an embodiment of the present application;
FIG. 8 is a second cross-sectional view schematically showing the internal structure of a culture tube according to an embodiment of the present application;
FIG. 9 is a schematic view of a partial explosion for illustrating a spacing mechanism in an embodiment of the present application;
fig. 10 is an enlarged view of a portion C in fig. 4.
Reference numerals illustrate:
1. A multi-terrain support;
2. A driving mechanism, a hydraulic cylinder and a hydraulic cylinder;
3. Pressing down a rod;
4. culture tube 41, culture cavity 411, first guide groove 412, second guide groove 42, first tube wall 421, embedding groove 43, second tube wall 44, mounting groove 45, arc-shaped groove 451, vertical groove section 452, arc-shaped groove section 453, transverse groove section 46, through cavity 461, through subchamber 462, avoiding subchamber 47, arc-shaped bending channel;
5. mud column dividing mechanism, 51, push-pull assembly, 511, first electric cylinder, 512, pushing plate, 5121, sliding groove, 5122, clamping groove, 52, bendable dividing assembly, 521, connecting metal sheet, 522, bendable piece, 5221, spring steel sheet, 53, guiding assembly, 531, first guiding plate, 532, second guiding plate, 533, guiding cavity;
6. An anti-drop mechanism; 61, a second electric cylinder, 62, a bendable blocking assembly, 621, a round rod, 622, and a rubber sealing layer;
7. the device comprises a limiting mechanism, a limiting steel pipe, a 711 arc-shaped strip hole, a 72 rotating column, a 721 rectangular groove, a 73 limiting block, a 74 rubber ring, a 75 detachable driving piece, a 751 rotating handle, a 752 and a plug-in rod;
8. and (5) an aseptic culture bin.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. It will be apparent that the described embodiments are only some, but not all, embodiments of the application. All other embodiments, which can be made by a person skilled in the art without any inventive effort, are intended to be within the scope of the present application based on the embodiments of the present application.
The application provides a portable layered collecting and culturing device for a wetland plant seed warehouse, referring to fig. 1 to 4, which comprises a multi-terrain support frame 1, a driving mechanism 2, a pressing rod 3, a culturing pipe 4, a mud column dividing mechanism 5 arranged in the pipe wall of the culturing pipe 4 and an anti-falling mechanism 6 arranged at the inlet of a culturing cavity 41 in an opening and closing manner.
Illustratively, the multi-terrain support frame 1 is used as a mounting foundation, and can be matched with adjustable support legs, anchoring piles or floating pads to provide stable support on different terrains such as flat bottom, gentle slope, soft mud and the like, so that the multi-terrain support frame is convenient to rapidly deploy and reset under field conditions. Further, foundation bolts can be further arranged at the support legs of the multi-terrain support frame 1, so that the multi-terrain support frame is conveniently embedded into the ground, and the installation stability of the multi-terrain support frame 1 is improved.
Illustratively, the driving mechanism 2 is detachably mounted on the multi-terrain support frame 1, and an electric push rod, a hydraulic jack or a worm and worm gear hand-operated assembly is selected as a power source, and axial pressure is output to the downward pressure rod 3 through the driving end.
Illustratively, the first end of the pressing rod 3 is detachably mounted on the driving end of the driving mechanism 2, specifically, the first end of the pressing rod 3 may be connected with the driving end of the driving mechanism 2 through a quick locking structure, a threaded connection or a clamping connection, etc., the second end of the pressing rod 3 is detachably connected with the culture tube 4 coaxially, the pressing rod 3 transfers a pressing force along an axis during the operation of the driving mechanism 2, the culture tube 4 is vertically pressed into the wetland substrate, the culture cavity 41 and the mud form a relatively sealed containing space during the penetration process, and a mud column sample with complete shape is gradually generated.
Illustratively, a plurality of mud column dividing mechanisms 5 are arranged in the wall of the culture tube 4, the mud column dividing mechanisms 5 are configured to have at least a first state and a second state, the mud column dividing mechanisms 5 are accommodated in the wall of the culture tube 4 when the mud column dividing mechanisms 5 are in the first state, the mud column dividing mechanisms 5 are radially arranged in the culture cavity 41 to divide mud columns in the culture cavity 41 when the mud column dividing mechanisms 5 are in the second state, and the anti-falling mechanisms 6 are arranged in an opening and closing mode and are used for opening or closing inlets of the culture cavity 41.
It can be understood that the female operator or the personnel with smaller physical strength can finish the seed warehouse collection and culture operation of the deep soil under the assistance of the structure and the lower physical strength, so that the operation adaptability and the man-machine friendliness are improved to a certain extent. After the sampling is completed, the anti-disengaging mechanism 6 closes the inlet of the culture chamber 41. After the culture tube 4 is detached from the pressing rod 3, the mud column dividing mechanism 5 enters a second state to radially divide the mud column in the culture cavity 41, the mud column dividing mechanism 5 is arranged in the tube wall of the culture tube 4 at a plurality of equal intervals or according to a preset layer thickness, can be stored in the tube wall from the first state to be converted into a second state to extend into the culture cavity 41, form circular cutting or semi-circular cutting edges, divide the mud column into a plurality of layers of seed samples along the sampling depth direction, and each layer of seed sample corresponds to in-situ depth information, so that the follow-up split charging, weighing, numbering and analysis according to the target layered thickness are facilitated.
Meanwhile, after the segmentation action is completed, an operator opens the anti-falling mechanism 6 in a homeotropic manner, so that the subsamples closest to the inlet of the culture cavity 41 slide out of the culture tube 4 under the action of dead weight or slight pushing in sequence from bottom to top, then the rest subsamples are released section by section, no additional overturning or external cutting is needed in the whole process, the disturbance degree of a layering interface is low, and the split charging efficiency is remarkably improved.
It should be noted that, in order to avoid understanding ambiguity caused by names such as "multi-terrain support frame 1", "driving mechanism 2", "pressing rod 3", "culture tube 4", "culture cavity 41", "mud column dividing mechanism 5" and "anti-drop mechanism 6" at different positions, in this embodiment, the names correspond to the same name parts in the claims, the first state refers to a rest state in which the mud column dividing mechanism 5 is housed inside the wall of the culture tube 4 and does not invade the culture cavity 41, the second state refers to a working state in which the mud column dividing mechanism 5 enters the culture cavity 41 in a radial direction and is used for dividing the mud column, and the opening of the anti-drop mechanism 6 provides a channel for the inlet of the culture cavity 41 to allow the mud column or sub-sample to enter and exit, and is closed so that the inlet of the culture cavity 41 is blocked and no longer allows the sample to slide out.
In conclusion, the overall device has the advantages of light weight, layering precision, sample column integrity and operation efficiency to a certain extent, and has stronger engineering practical value and popularization potential for the layered culture work of the wetland plant seed library, through the terrain adaptability provided by the multi-terrain support frame 1, the axial pressing force transmitted by the driving mechanism 2 and the pressing rod 3, the coaxial penetrating sampling culture path formed by the culture tube 4, the sectional quantitative cutting effect brought by the mud column dividing mechanism 5 and the stable holding effect of the extraction period provided by the anti-disengaging mechanism 6.
Illustratively, the lower pressure rod 3 and the culture tube 4 are made of aviation-grade aluminum alloy materials, and the materials have the characteristics of high strength and light weight, so that the weight of the device can be effectively reduced on the premise of ensuring the overall rigidity, and convenience in field transportation and handheld operation is improved. The lower pressure rod 3 is connected with the culture tube 4 through a quick-release buckle structure, and an operator can quickly realize connection and disassembly through a push button, a rotary clip or a push type mechanism, so that the culture tube 4 can be quickly replaced or cleaned after sampling is finished, the waiting time is reduced, and the working efficiency is improved. Meanwhile, the opening at the lower end of the culture tube 4 is a diamond coating tube head, and the wall thickness of the edge of the end of the tube head is thinned, so that the culture tube 4 can be easily inserted into soil.
Further, the inner diameter of the culture chamber 41 is preferably 8cm, and the length of the culture chamber 41 is in the range of 30cm to 45cm, and the parameters can be adjusted according to practical scientific research requirements. In particular, the above-mentioned parameter ranges are based on typical depth layer designs of the vertical distribution of most wetland plant seed banks, enabling a reasonable balance between sampling volume, personnel portability and earth column integrity. Two groups of mud column dividing mechanisms 5 are arranged in the culture cavity 41 along the depth direction, the two mud column dividing mechanisms are distributed at equal intervals and divide the sampling column into three sections according to the length of the culture cavity 41, the length of each section is controlled between 10cm and 15cm, higher layering resolution is facilitated, each sub-sample can represent a relatively independent soil layer structure, and fine analysis on seed abundance distribution, sediment properties and historical vegetation succession information is facilitated.
In some embodiments, referring to fig. 4, 5 and 6, the culture chamber 41 is eccentrically disposed in the axial direction within the culture tube 4, and specifically, the tube walls of the culture tube 4 located at both sides of the culture chamber 41 form a first tube wall 42 and a second tube wall 43 having different thicknesses from each other, wherein the thickness of the first tube wall 42 is greater than the thickness of the second tube wall 43. This structural design is advantageous in that it spatially reserves enough arrangement positions for mounting the push-pull assembly 51, and further, a mounting groove 44 is provided in the inner axial direction of the first tube wall 42, which mounting groove 44 is used for accommodating the push-pull assembly 51. Meanwhile, an arc groove 45 communicated with the mounting groove 44 is further formed in the first pipe wall 42, the arc groove 45 is gradually bent and extends to the inside of the culture cavity 41 along the radial direction, a first end of the arc groove 45 is communicated with the mounting groove 44, a second end of the arc groove 45 is communicated with the culture cavity 41, and the groove width of the arc groove 45 is smaller than that of the mounting groove 44. The bendable dividing member 52 is made of a material capable of being elastically bent, is in a bent storage state when not driven by the push-pull member 51, and is fitted into the mounting groove 44 and the arc groove 45 in accordance with the structural shape thereof.
It will be appreciated that when the push-pull assembly 51 is not in operation, the bendable split assembly 52 is entirely within the mounting slot 44 and the arcuate slot 45, which is defined as the first state. In this state, the bendable partition assembly 52 is completely accommodated in the structural space inside the wall of the culture tube 4, and does not enter the culture cavity 41, so that the continuity, structural integrity and column stability of the culture soil sample in the culture cavity 41 are not disturbed, and the acquisition of the relatively original undisturbed mud column sample is facilitated. After the sampling operation is completed, the operator can drive the push-pull member 51 to push the bendable dividing member 52 partially from the arc-shaped groove 45 into the culture chamber 41, which is the second state. Because the bendable dividing assembly 52 moves along the arc-shaped groove 45 and enters the culture cavity 41 at the outlet, the overall path of the dividing assembly has a certain curvature change, so that the dividing assembly material needs to have good flexibility and a certain rigidity, and can stably extend into the culture cavity 41 along a preset track under the action of push-pull force and form an effective dividing interface.
In the second state, the insertion position of the bendable dividing assembly 52 is matched with the preset sampling depth in the culture cavity 41, so that the mud column sample can be divided along the radial direction, a plurality of sub-sample areas are constructed, and the collection, numbering and analysis of the subsequent sub-samples are more efficient and accurate. Through setting up mud post cutting mechanism 5 in eccentric thick wall district, both satisfied the structural strength demand, do not influence the central symmetry and the stability of mud post again, also do benefit to through the controllability of structure guide bendable segmentation subassembly 52 direction of motion, improve the precision and the uniformity of cutting apart the operation. The design scheme ensures that the whole segmentation operation can be completed in one step after the sampling is completed by reasonably setting the eccentric structure of the culture cavity 41 and combining the coordinated motion mode of the multi-cavity embedded part, thereby reducing the operation intensity of operators and having higher practical value and adaptability in the field environment.
In some embodiments, referring to fig. 4, 5 and 6, the push-pull assembly 51 includes a first electric cylinder 511 and a push plate 512, the first electric cylinder 511 is mounted inside the mounting groove 44 through a quick-release connector, an axial extension direction of a piston rod of the first electric cylinder 511 is consistent with a length direction of the mounting groove 44, and the push plate 512 is slidably disposed in the mounting groove 44 and is connected with the piston rod of the first electric cylinder 511 through a connection structure, so that the push plate 512 can be directly driven to move along an axial direction of the mounting groove 44 when the first electric cylinder 511 drives the piston rod to reciprocate. Further, the bendable dividing assembly 52 is composed of a connecting metal sheet 521 and a bendable member 522, wherein one end of the connecting metal sheet 521 is fixedly connected to the end of the pushing plate 512 away from the first electric cylinder 511, and the other end is connected to the bendable member 522, so that the bendable dividing assembly 52 can move integrally therewith during the moving process of the pushing plate 512.
Illustratively, the arcuate slot 45 is disposed within the first tube wall 42, and is specifically comprised of a vertical slot segment 451, an arcuate slot segment 452, and a transverse slot segment 453. Wherein the vertical groove section 451 is disposed on the same straight line with the mounting groove 44, so that the connection metal sheet 521 can smoothly enter in the axial direction. The arc groove section 452 has a certain curvature, and two ends are respectively communicated with the vertical groove section 451 and the horizontal groove section 453, and the horizontal groove section 453 is radially opened towards the culture cavity 41 and communicated with the culture cavity 41, so that a movable path of the bendable partition assembly 52 is constructed. The connecting metal sheet 521 is designed to be an elongated strip, has certain axial rigidity and transverse flexibility, the moving path of the connecting metal sheet 521 is mainly limited in the vertical groove section 451 and the mounting groove 44, the bendable piece 522 is made of a sheet or strip-shaped flexible material, and has good bending adaptability and deformability, and the working path of the connecting metal sheet 521 covers the vertical groove section 451, the arc-shaped groove section 452 and the transverse groove section 453 until reaching the space in the culture cavity 41.
It will be appreciated that in the splitting operation, the first cylinder 511 is activated and its piston rod is extended such that the push plate 512 slides downwardly from the upper portion of the mounting slot 44, bringing the connecting metal sheet 521 vertically into the vertical slot section 451. As the connecting metal sheet 521 advances gradually, the bendable member 522 is driven by the connecting metal sheet 521 to rotate from the vertical groove section 451 to the arc groove section 452 and further enter the transverse groove section 453, and finally slides radially into the culture chamber 41 from the end of the transverse groove section 453. Because the arcuate slot 452 has a degree of curvature, the flexible member 522 automatically adapts to the curvature of the channel to bend as it passes through the slot, and when it enters the culture chamber 41, it stretches in a radial direction to form an effective cutting surface, and by means of continuous pushing, it is possible to perform a radial cutting operation on the mud column sample at a predetermined position in the culture chamber 41, thereby dividing the soil sample subsections of multiple depth layers.
Based on this, this structure is through setting up multistage channel structure and flexible piece 522 cooperation of buckling accurately, can accomplish layering cutting operation under the circumstances that does not interfere whole sample structure after the sampling, promotes sample segmentation precision, alleviates operator's operation burden. Meanwhile, the rigid-flexible linkage between the pushing plate 512 and the connecting metal sheet 521 enables the partition to be completely contained in the mounting groove 44 and the vertical groove section 451 in a non-working state, and does not enter the culture cavity 41 in the sampling stage, and does not cause disturbance to the mud column sample, thereby being beneficial to the stability and representative maintenance of the sample. The whole structure has high adaptability and definite operation path, and is suitable for sampling, culturing and applying the seed bank under the complex environment of the field wetland.
In some embodiments, referring to fig. 4 and 5, in order to improve the movement stability of the connecting metal sheet 521 during the separation process and prevent the deflection phenomenon caused by the sheet structure thereof during the vertical pushing process, the mud column separation mechanism 5 further comprises a guiding component 53. The guide assembly 53 includes a first guide plate 531 and a second guide plate 532, where the first guide plate 531 and the second guide plate 532 are installed on the inner end wall of the installation groove 44 in a parallel manner, and are respectively disposed on two sides of the vertical groove section 451, and a guide cavity 533 with a through middle portion is formed by symmetrical arrangement. The width of the guide cavity 533 is consistent with the width of the vertical groove section 451, and the thickness of the connecting metal sheet 521 is consistent with the widths of the guide cavity 533 and the vertical groove section 451, so that the connecting metal sheet 521 can slide along the axial direction of the guide cavity 533 and is always in a preset sliding path, and the phenomenon of influence force transmission such as transverse offset or bending is not easy to occur in the vertical pushing process.
In addition, in order to prevent the pushing plate 512 from interfering with the first guide plate 531 and the second guide plate 532 in the downward moving process, two sliding grooves 5121 are formed on the pushing plate 512, and the sizes and positions of the two sliding grooves 5121 are matched with those of the first guide plate 531 and the second guide plate 532, so that the two guide plates are respectively inserted into the corresponding sliding grooves 5121 when the pushing plate 512 moves downward, and the movement path of the pushing plate 512 and the existence of the guide assembly 53 are not interfered with each other. This structural arrangement maintains the structural compactness and movement smoothness of the entire push-pull assembly 51 while ensuring the guiding function.
In combination with the above structure, during the movement of the connecting metal sheet 521 driven by the pushing plate 512, the connecting metal sheet 521 is restrained by the guide plates at both sides of the guide cavity 533, so that the connecting metal sheet 521 can always keep a straight state during the vertical pushing stage, thereby being beneficial to stably and effectively transmitting the pushing force to the bendable member 522 at the distal end of the connecting metal sheet 521. Meanwhile, the guide component 53 does not block the original structural movement, so that the pushing plate 512 can smoothly complete the vertical stroke, which is beneficial to maintaining the continuity and reliability of the whole push-pull component 51 in actual operation. The guiding component 53 is beneficial to improving the accuracy and reliability of the segmentation action, and is particularly suitable for the layered culture working condition of the wetland sample, which needs to preserve the structural integrity of the mud column with high precision.
In some embodiments, referring to fig. 5, the bendable member 522 is formed by a spring steel plate 5221 with a strong elastic resilience, and one end of the spring steel plate 5221 is connected to the distal end of the connecting metal plate 521 by welding, and the other end can enter the culture chamber 41 under the action of pushing force. In order to make the movement of the spring steel sheet 5221 in the culture cavity 41 more controllable, and to effectively cut off the mud column after entering the culture cavity 41, a first guide groove 411 is annularly arranged on the inner wall of the culture cavity 41, the first guide groove 411 is arranged in a closed annular shape along the circumferential direction of the culture cavity 41, the first guide groove 411 is structurally communicated with the transverse groove section 453, and the groove width and the groove depth of the first guide groove 411 are matched with the size parameters of the spring steel sheet 5221, so that the spring steel sheet 5221 can smoothly enter the groove cavity of the first guide groove 411 along the guide path after being acted by the push-pull assembly 51. It should be noted that, in order to facilitate the display of the shape of the spring steel sheet 5221, the spring steel sheet 5221 is intentionally drawn to be thicker in fig. 4 and 5, and in fact, the thickness of the spring steel sheet 5221 in fig. 4 and 5 should not be considered as the actual thickness of the spring steel sheet 5221, and the thickness of the actual spring steel sheet 5221 is in the range of 0.5mm to 1mm.
It will be appreciated that the first guide slot 411 serves to provide a stable insertion path for the spring steel plates 5221 to pass through the culture chamber 41 in a radial direction to intercept the mud column within the culture chamber 41. The presence of the first guide groove 411 can effectively restrict the swing of the spring steel piece 5221, which is beneficial to improving the cutting accuracy. In addition, the cross section of the first guide slot 411 may be rectangular or circular, and the inner wall of the first guide slot 411 is coated with polytetrafluoroethylene material, which has a low friction coefficient, and helps the spring steel sheet 5221 to slide into the guide slot smoothly, and extrudes residual soil or impurities in the guide slot during the sliding process, so that the sliding path of the spring steel sheet 5221 is not easy to be blocked. In order to enhance the cleaning convenience, a smaller bending structure is arranged between the lower groove wall of the guide groove and the inner wall of the culture cavity 41, which is beneficial to reducing the accumulation risk of impurities at the bottom of the annular groove, and the auxiliary culture tube 4 is more efficient in cleaning and maintaining operation after completing sample collection and segmentation.
In summary, the structure setting for spring steel sheet 5221 can accurately slide into the first guiding groove 411 of cultivateing chamber 41 under the effect of push-pull subassembly 51, and radially cut off the sample post along cultivateing chamber 41, promote the accuracy of layering segmentation, and then in investigation and the sampling of wetland sample seed storehouse, promote effectiveness and the representativeness that layering sample obtained, the soil structure difference under the adaptation multi-type wetland environment possesses better adaptability and practicality.
In some embodiments, referring to fig. 4, 5 and 6, to further improve the safety and reliability of the portable wetland plant seed magazine layered collection and culture device in actual use, a limiting mechanism 7 is added to the device for limiting the axial movement of the pushing plate 512 in the push-pull assembly 51. The limiting mechanism 7 comprises a limiting steel pipe 71, a rotating column 72, a limiting block 73, a rubber ring 74 and a detachable driving piece 75. The limiting steel tube 71 is fixedly connected in the axial direction in the embedded groove 421 axially arranged in the first tube wall 42, and an arc-shaped strip hole 711 is formed through the limiting steel tube to match with the rotation of the subsequent limiting block 73 to enter and exit. The rotating column 72 is rotatably inserted in the limiting steel pipe 71 and fixedly connected with the limiting block 73, and the rubber ring 74 is embedded between the rotating column 72 and the limiting steel pipe 71, so that the rotating tendency of the rotating column 72 under the condition of no external force driving is limited through friction, and the limiting block 73 is prevented from being separated from the limiting position by no person. Referring to fig. 7 and 8, a through cavity 46 is provided in the first pipe wall 42, the through cavity 46 has a through sub-cavity 461 and a avoiding sub-cavity 462, and the through sub-cavity 461 is configured to communicate with and face the clamping groove 5122 when the mud column dividing mechanism 5 is in the first state, and the avoiding sub-cavity 462 communicates with one side of the through sub-cavity 461.
For example, in order not to affect the appearance integrity and stability of the device, the length of the limiting steel tube 71 and the rotating post 72 is designed to be smaller than the depth of the embedded groove 421, so that the limiting steel tube is integrally accommodated in the embedded groove 421 of the first tube wall 42, which is convenient for compact structure and low sensitivity to external interference.
As an example, referring to fig. 7 and 8, the setting position of the limiting block 73 is aligned with the through subchamber 461, the through subchamber 461 is communicated with the arc-shaped strip hole 711 and faces the clamping groove 5122 on the pushing plate 512, when the pushing plate 512 is in the initial non-action state, the clamping groove 5122 on the pushing plate 512 is aligned with the through subchamber 461, and the rotating post 72 drives the limiting block 73 to rotate into the clamping groove 5122 at this time, so that an axial limit is formed on the pushing plate 512, so that an operator cannot malfunction before not being ready to push down the spring steel sheet 5221 for the splitting operation, and the damage risk of the improper operation on the culture tube 4, the spring steel sheet 5221 and the sample integrity is reduced to a large extent. When the stopper 73 rotates along the arc-shaped strip hole 711 from the through subchamber 461 to the avoiding subchamber 462, the stopper 73 is separated from the clamping groove 5122, so that the pushing plate 512 can move normally and axially.
Illustratively, referring to fig. 6 and 9, the detachable driving member 75 includes a rotating handle 751 and a plugging rod 752, wherein the rotating handle 751 is vertically connected to the plugging rod 752, the plugging rod 752 has a rectangular cross-section, the rotating post 72 is axially provided with a rectangular slot 721, and the plugging rod 752 is configured to be plugged with the rectangular slot 721. When the operator needs to release the limit state and perform the dividing operation, he can mechanically engage the rectangular groove 721 of the rotating post 72 through the insertion rod 752. The cross section of the inserting rod 752 is rectangular, and after the inserting rod is in inserting fit with the rectangular groove 721 axially formed in the rotating column 72, the rotating handle 751 faces the outside of the culture tube 4, so that a controllable structure which is convenient for manual operation is formed. Rotating the handle 751 drives the rotating post 72 to angularly displace, so that the limiting block 73 rotates from the through subchamber 461 to enter the avoiding subchamber 462, and is separated from the clamping groove 5122 of the pushing plate 512, and the pushing plate 512 is restored to the downward movable state at this time.
It will be appreciated that with the above arrangement, the push plate 512 remains locked until unauthorized unlocking, and no misoperation can be performed when the spring steel sheet 5221 is still in the folded state and no sample is in the culture chamber 41, thereby further improving the controllability and standardization in the sampling process.
Further, to improve the matching adaptability of the stopper 73 and the clamping groove 5122, the end of the stopper 73 close to the clamping groove 5122 adopts a circular arc structural design, which is beneficial to smoothly entering or exiting the clamping groove 5122 in the rotation process of the stopper 73, and reduces the clamping stagnation phenomenon caused by the unmatched structure. Meanwhile, the rubber ring 74 arranged between the limit steel pipe 71 and the rotating column 72 is beneficial to keeping the rotating column 72 stably existing in a desired angle state by increasing the rotation friction, and reduces the probability of the rotating column 72 deflecting and further false triggering of the limit state caused by vibration, carrying or accidental collision.
The mechanical limiting and manual unlocking mechanism is introduced on the basis of compact structure and simple operation, the sampling flow is guided from the structural path, and a good foolproof effect is provided. For users with insufficient first use or operation experience, even if the user does not read the description in detail, the user can easily follow the structural instruction to perform the correct operation steps, thereby remarkably reducing the misoperation rate and being beneficial to improving the popularization and user friendliness of the device. In addition, the limiting mechanism 7 also effectively protects the bendable segmentation assembly 52, particularly the spring steel sheet 5221 from being erroneously pushed into the culture cavity 41 when not being sampled, prolongs the service life of the whole device and the sampling accuracy of layered samples, and further supports scientific research and ecological restoration work of the wetland plant seed library under the environment evolution background.
In some embodiments, referring to fig. 4 and 10, the anti-disengaging mechanism 6 includes a second electric cylinder 61 and a bendable blocking assembly 62, wherein the second electric cylinder 61 is detachably mounted in the mounting groove 44 for applying an axial pushing force to the bendable blocking assembly 62. The mounting groove 44 communicates with the culture chamber 41 through an arcuate flex channel 47, the arcuate flex channel 47 providing a spatial path for the flexible barrier member 62 to be deflected radially from the mounting groove 44 and into the interior of the culture chamber 41 adjacent the inlet.
Illustratively, the bendable blocking assembly 62 includes a plurality of round bars 621 and a rubber sealing layer 622 covering the outer portions thereof, wherein each round bar 621 is arranged in parallel along the length direction of the mounting groove 44, and two adjacent round bars 621 adopt a relatively rolling fit manner, so that the whole bendable blocking assembly 62 has a certain flexible bending capability. Under the action of the pushing force, the bendable blocking assembly 62 can follow the path of the arc-shaped bending channel 47 to axially change into radially enter the second guide groove 412 on the inner wall of the culture cavity 41. The second guiding groove 412 is an annular structure, is circumferentially arranged along the inner wall of the culture cavity 41, and has a size matching the cross-sectional shape of the bendable blocking component 62, and is used for accommodating the bendable blocking component 62 to form a radial blocking structure at the inlet of the culture cavity 41.
With the above structure, when the second electric cylinder 61 is started, the piston rod thereof drives the bendable blocking assembly 62 to move along the mounting groove 44, and then turns along the bending channel and enters the second guiding groove 412. Under the constraint of the second guide groove 412, the bendable blocking component 62 is converted from a flexible state to a relatively rigid arc-shaped strip state, so that a stable sealing surface is formed at the inlet of the culture cavity 41, thereby playing a role in blocking the mud column sample formed inside, and greatly reducing the probability of the sample sliding off from the mouth of the culture cavity 41 in the process of carrying and moving.
In terms of material selection, the round bar 621 is made of a relatively high-strength and light-weight structural material, provides sufficient supporting strength, reduces sliding friction through a circular section, and the rubber sealing layer 622 is coated on the outer surface of the round bar for improving sealing effect and absorbing certain soil pressure so as to reduce direct extrusion action of the structural member. Because the bottom of the culture chamber 41 may bear the entire weight of the mud column sample, the bendable blocking assembly 62 is structurally stronger than the bendable members 522 for segmentation and does not have to be designed as an extremely thin structure, thereby improving its overall load bearing capacity and service life.
In addition, the working process of the anti-disengaging mechanism 6 and the mud column dividing mechanism 5 have a certain commonality in the structural path, and both relate to the driving and the position control of the bendable member 522 or the bendable blocking assembly 62, but there are essential differences in the purpose and the structural constitution. The anti-disengaging mechanism 6 is focused on the sealing of the inlet of the culture cavity 41 and mainly bears the axial gravity and the extrusion force of the mud column, and does not require parts to go deep into the mud column for cutting, so that a soft and rigid sealing surface is formed more conveniently by adopting the structure of the multi-round rod 621 and the rubber sealing layer 622, and the anti-disengaging mechanism has good stress dispersion and repeatable driving and withdrawing capabilities. Meanwhile, because the bendable blocking component 62 is thicker than the bendable piece 522, the inner diameter of the arc bending channel 47 is larger, the provided rubber sealing layer 622 can be in extrusion fit with the inner wall of the arc bending channel 47 in the sliding process, so that soil in the culture cavity 41 is prevented from excessively entering the arc bending channel 47 through a gap between the rubber sealing layer 622 and the arc bending channel 47, and meanwhile, the outer surface of the rubber sealing layer 622 can be coated with a polytetrafluoroethylene layer, so that the friction force between the inner wall of the arc bending channel 47 and the outer surface of the rubber sealing layer 622 is reduced, and the bendable blocking component 62 can slide in the arc bending channel 47 more smoothly.
In summary, the embodiment provides the anti-disengaging mechanism 6 with structural stability, operation convenience and use reliability by providing the bendable blocking component 62 with flexible path following capability and certain rigidity and combining with a proper electric driving mechanism, which is greatly beneficial to improving the sample integrity maintaining capability of the device in the wetland field sampling process and also gives consideration to the sealing durability and convenient cleaning performance in the repeated use process.
In some other alternative embodiments, in order to realize remote control operation of the first electric cylinder 511 and the second electric cylinder 61, a wireless remote control and electric control execution system can be additionally arranged in the portable wetland plant seed bank layered collection and cultivation device, and the wireless remote control and electric control execution system comprises a handheld remote controller, a 2.4GHz or BLE wireless transceiver module (which is arranged in a cultivation tube 4 side control box or a rack control box), a main control board (MCU (such as STM32/ESP 32) and a microcontroller with wireless or serial peripheral), a power management module and an independent storage battery, a double H-bridge or electric cylinder special driving board, a position and travel detection unit (travel switch, hall element or magnetic encoder), an emergency stop button and power-on self-checking watchdog circuit, an address pairing and simply encrypted communication protocol stack, a status indication and audible-visual alarm unit, an external antenna and a waterproof connector.
It can be understood that the main control board receives the instructions of start-stop, travel, speed grade, moment/current limiting and the like issued by the handheld remote controller, outputs control signals to the first electric cylinder 511 or the second electric cylinder 61 through the driving board, performs closed-loop or semi-closed-loop control by combining travel switch, encoder or current sampling data to complete actions such as push, pull, pressure maintaining, soft start-stop and the like, the wireless transceiver module supports pairing and address coding to reduce crosstalk risks among different devices, the power management module manages charge and discharge, undervoltage protection and low power consumption standby of the storage battery to enable the storage battery to have longer standby duration under field working conditions, the emergency stop button and the watchdog cut off driving output when communication is abnormal, program runs or current is abnormally increased to maintain a relatively safe state, and the state indicator or the buzzer gives a prompt in the scenes such as action completion, in-place, out-of-limit, undervoltage and the like, so that single person long-distance operation is facilitated.
By introducing the wireless control component of the mature circuit, key steps such as layering segmentation, inlet plugging and the like of the first electric cylinder 511 and the second electric cylinder 61 can be remotely triggered and finely controlled, operators do not need to repeatedly bend over and press in a soft mud or water accumulation area in a short distance manually, the phenomenon of uneven labor intensity and stress is relieved to a certain extent, parameterized setting of travel, speed and moment limiting is beneficial to reducing the influence of misoperation on the integrity of the spring steel sheet 5221, the bendable blocking component 62 or the mud column, and multistage limiting (mechanical limiting mechanism 7+travel sensing+current threshold) and emergency stop redundancy configuration enable the device to have higher operation fault tolerance space and repeatability in a complex wetland environment, and a wireless pairing and equipment address management mode supports simultaneous operation of multiple sets of devices in the same sampling zone, so that the construction organization efficiency is obviously improved.
In some embodiments, referring to fig. 1,2 and 3, the driving mechanism 2 includes a hydraulic cylinder 21, where the hydraulic cylinder 21 is coaxially connected to an end of the pressing rod 3 through a piston rod, and a movement direction of the piston rod of the hydraulic cylinder 21 is consistent with an axial direction of the pressing rod 3. The operator can activate the hydraulic cylinder 21 to make the piston rod of the hydraulic cylinder 21 stably extend downward. Because the piston rod of the hydraulic oil cylinder 21 is fixedly connected with the pressing rod 3, the pressing rod 3 can be synchronously driven to move downwards along the vertical direction, and the culture tube 4 is further driven to be inserted into the wetland substrate sludge.
It can be understood that the hydraulic cylinder 21 in the above structure can be provided with a remote control sensing module, and an operator can operate the hydraulic cylinder 21 by a hand-held remote controller in the open air, and the conventional electric control implementation manner of the hydraulic cylinder 21 is omitted here. In the practical test process, an operator only needs to apply about 15kg of hand force to the hydraulic cylinder 21, and can assist to push the downward pressing rod 3 to move downwards and penetrate through wet surface layer soft mud with a depth of more than 30cm, so that the operating force is reduced by about 70% compared with the traditional mode of directly pressing and holding the structure manually, and the hydraulic cylinder is particularly suitable for female researchers or application scenes with higher operating fatigue strength during long-time work.
The multi-terrain support 1 is provided with a riding board, an operator can also ride on the riding board, and apply pressure to the multi-terrain support 1 through self gravity, so that partial self weight of the operator can resist thrust generated when a piston rod of the hydraulic oil cylinder 21 stretches, and the whole equipment can stably run, and the operator can not need to ride on the riding board if the supporting legs on the multi-terrain support 1 are fixed on the ground through foundation bolts, and the connecting force between the supporting legs on the multi-terrain support 1 and the ground can resist thrust generated when the piston rod of the hydraulic oil cylinder 21 stretches.
The pressing rod 3 can be made of aviation-grade aluminum alloy materials, has the characteristics of light weight and high rigidity, and is convenient for a hydraulic driving system to push the pressing rod for a long stroke so as not to easily generate bending or deformation. The hydraulic oil cylinder 21 provides stable and uniform thrust in the force application process, so that the attitude verticality of the culture tube 4 in the process of inserting the wetland substrate sludge is maintained, and the integrity and layering accuracy of the sludge column sample are improved. Meanwhile, the driving mode converts small-amplitude manual operation into continuous and stable high-thrust output through a hydraulic oil pressure gain principle, and improves sampling efficiency and stability while reducing the burden of an operator.
In some embodiments, in combination with fig. 1, 2 and 4, in order to improve the purity of the samples sampled by layers of the wetland plant seed library and the controllability of subsequent experiments, the portable device for collecting and culturing the wetland plant seed library by layers is further provided with a sterile culture bin 8. The sterile culturing chamber 8 is an independent component, and is configured to be sleeved at the inlet end of the culturing tube 4, namely, the opening position of the culturing cavity 41 communicated with the external environment. The sterile culture bin 8 is made of aluminum box materials, has the characteristics of good light weight and sealing performance, and is suitable for rapid assembly and disassembly in a field environment. The interior of the sterile incubation chamber 8 is pre-filled with an inert gas, such as nitrogen or helium, to create a low oxygen, non-reactive atmosphere that is conducive to isolating the sample from the environment prior to the sample entering the incubation cavity 41, thereby reducing to some extent the likelihood of contact of the sample surface with external oxygen, microorganisms or contaminating factors.
In addition, a laser recording coding layer is arranged on the outer surface of the box cover of the sterile culture bin 8 and used for marking different sampling depths or position information, for example, the sampling depth range is represented in a W20-25cm mode, so that an experimenter can conveniently and rapidly identify and trace the source of a sample after sampling is completed. The aseptic culture bin 8 is used as a front-end additional structure, is matched with the inlet of the culture tube 4 in a sealing way through a sleeved connection configuration, does not interfere with the original structure of the sampling device, and does not change the sampling action flow, so that the micro-environment control capability in the sample collection process is improved, the aseptic culture bin can be flexibly detached or replaced as required in the actual sampling process, and the aseptic culture bin is suitable for scenes with higher aseptic requirements on samples in scientific research experiments.
In some embodiments, each component of the portable wetland plant seed warehouse layered collection and culture device adopts a modularized detachable design, and specifically comprises a pressing rod 3, a culture tube 4, a multi-terrain support frame 1, a mud column dividing mechanism 5, a disengaging mechanism 6, a driving mechanism 2, a sterile culture bin 8 and other functional unit components which are assembled with each other in a quick-dismantling buckle, threaded connection or splicing structure and the like, so that the portable wetland plant seed warehouse layered collection and culture device is convenient to quickly assemble before sampling operation and disassemble and store after operation is completed. In addition, in order to meet the actual requirements of portability and light weight in the field wetland environment, the functional components are preferably made of light materials, for example, the pressure-down rod 3, the culture tube 4 and the support frame main body are formed by extrusion molding of aviation-grade aluminum alloy, the connecting structure and the small-sized components are made of polycarbonate or reinforced nylon materials, the first electric cylinder 511 and the second electric cylinder 61 are compact and light-weight packaging schemes, and all the components have quality control on the premise of ensuring strength and stability.
Through the whole machine weight statistics, the total weight of the whole machine is only 4.2kg, and a single person has no obvious burden to bear, so that the whole machine is suitable for portable sample layered culture operation under complex terrains such as wetlands, marshes, shoals and the like, and the suitability and the maneuvering flexibility of the whole machine in field practical application are improved.
In the description of the present application, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more features. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the foregoing embodiments, the descriptions of the embodiments are emphasized, and for parts of one embodiment that are not described in detail, reference may be made to the related descriptions of other embodiments.
The embodiments, the implementation modes and the related technical features of the application can be mutually combined and replaced under the condition of no conflict.
The foregoing is only a preferred embodiment of the present application, and is not intended to limit the present application in any way, but any simple modification, equivalent variation and modification made to the above embodiment according to the technical matter of the present application still fall within the scope of the technical solution of the present application.
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| CN113720638A (en) * | 2021-08-31 | 2021-11-30 | 水利部牧区水利科学研究所 | Original state soil collection system that can keep soil and plant roots integrity |
| CN119880497A (en) * | 2025-01-15 | 2025-04-25 | 中国地质调查局地球物理调查中心 | Black land surface matrix detection sampling device |
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| SU1350530A1 (en) * | 1984-12-21 | 1987-11-07 | Производственный И Научно-Исследовательский Институт По Инженерным Изысканиям В Строительстве Госстроя Ссср | Formation tester |
| JP2003083956A (en) * | 2001-07-06 | 2003-03-19 | Omron Corp | Soil property observation device |
| CN101571458B (en) * | 2008-04-29 | 2011-05-11 | 同方威视技术股份有限公司 | Hand-hold wiping and sampling device |
| EP3567360B1 (en) * | 2017-01-05 | 2023-06-28 | Shimadzu Corporation | Device for dividing sample-capture chip |
| CN113375964A (en) * | 2021-06-14 | 2021-09-10 | 毕广印 | Sample sampling device for land detection |
| JP2023034616A (en) * | 2021-08-31 | 2023-03-13 | サクラ精機株式会社 | Encapsulation device |
| CN116430010A (en) * | 2023-04-20 | 2023-07-14 | 上海勘测设计研究院有限公司 | Phase-splitting simulation device capable of carrying out layered cutting on bottom mud |
| CN119574191A (en) * | 2024-12-10 | 2025-03-07 | 水利部交通运输部国家能源局南京水利科学研究院 | A wetland soil stratification sampling device |
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| CN113720638A (en) * | 2021-08-31 | 2021-11-30 | 水利部牧区水利科学研究所 | Original state soil collection system that can keep soil and plant roots integrity |
| CN119880497A (en) * | 2025-01-15 | 2025-04-25 | 中国地质调查局地球物理调查中心 | Black land surface matrix detection sampling device |
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