CN1207308C - Ganoderma applanatum polysaccharide, its preparation and medicine composition using said compound as active component - Google Patents
Ganoderma applanatum polysaccharide, its preparation and medicine composition using said compound as active component Download PDFInfo
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- CN1207308C CN1207308C CN 02144604 CN02144604A CN1207308C CN 1207308 C CN1207308 C CN 1207308C CN 02144604 CN02144604 CN 02144604 CN 02144604 A CN02144604 A CN 02144604A CN 1207308 C CN1207308 C CN 1207308C
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- ganoderma applanatum
- ulcer
- stomach
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention discloses ganoderma applanatum polysaccharide and provides a preparation method of the ganoderma applanatum polysaccharide. The present invention has the characteristics that the content of the obtained ganoderma applanatum polysaccharide is high (more than 80%), the content of heavy metal is low (less than 5 ppm), and the pesticide residue is low (less than 10 ppb), wherein the content of neutral polysaccharide is about 52.0%, the content of acidic polysaccharide is about 31.2%, and the content of polypeptide is less than 5%. Simultaneously, the present invention provides medicine which adopts activity ganoderma applanatum polysaccharide components as main ingredients or a prescription of other kinds of medicine. The medicine can be used for preventing and treating gastric ulcer, acute gastritis, chronic gastritis, duodenal bulbar ulcer, or diseases, such as stomachache, hyperhydrochloria, etc., which are caused by other reasons. The medicine has the functions of relieving pain and resisting inflammation.
Description
Technical field:
The present invention discloses a kind of Ganoderma Applanatum Polysaccharides, its preparation method is provided simultaneously and is the pharmaceutical composition of activeconstituents with this compound, belongs to Chinese medicine extracts active ingredients agent applied technical field.
Background technology:
The old beef liver of artist's conk different name that the present invention is alleged is the sporophore of Ganoderma applanatum (pers) pat (Gancdermaapplatum (Pers.) Pat.).Ganoderma Applanatum Polysaccharides has the obvious suppression effect to tumour S-180." liver must be answered " made from Ganoderma Applanatum Polysaccharides has therapeutic action to hepatitis B patient.The present invention that is reported in about methods such as Ganoderma Applanatum Polysaccharides anti-gastric-ulcer, treatment gastritis finishes before not report; Ganoderma Applanatum Polysaccharides is a kind of mixed polysaccharide mixture, having the polysaccharide fraction of antiulcer agent, the effect of treatment gastritis, also be to separate first to obtain, and processing method is simple, suitable, active strong, the purity high (more than 80%) of the polysaccharide part of extracting is a kind of novel method that industrialization is produced that is suitable for.
Summary of the invention:
The invention provides a kind of Ganoderma Applanatum Polysaccharides with pharmaceutical use.
The present invention also provides the preparation method who extracts above-mentioned substance in the loblolly tree tongue therefrom, is applicable to suitability for industrialized production.
The present invention further provides with active Ganoderma Applanatum Polysaccharides component and be main medicine or be used to prevent and the treatment of stomach ulcer, acute gastritis, chronic gastritis, duodenal bulbar ulcer and analgesia, anti-inflammatory action etc. with the other medicines prescription.
Ganoderma Applanatum Polysaccharides obtains by following extracting method:
Method 1
With the Chinese medicine artist's conk is raw material, uses decocting 1～3 time, and each time is 1～3 hour, filter, merging filtrate concentrates, concentrated solution is put cold, adds ethanol and is 20% to containing the alcohol amount, and is centrifugal, discard throw out, it is 80% to containing the alcohol amount that supernatant liquor adds ethanol, leaves standstill 24 hours, suction filtration, throw out washs in right amount with dehydrated alcohol, drying, be ground into fine powder, get Ganoderma Applanatum Polysaccharides.
Method 2
With the Chinese medicine artist's conk is raw material, and with 20% alcohol reflux 3 times, each time is 3 hours, united extraction liquid, it is 80% to containing the alcohol amount that concentrating under reduced pressure, concentrated solution add ethanol, leaves standstill suction filtration 24 hours, throw out washs in right amount with dehydrated alcohol, and drying is ground into fine powder, gets Ganoderma Applanatum Polysaccharides.
Aforesaid method of the present invention can be used for the polysaccharide fraction that a large amount of preparations of industrialization have the antiulcer agent isoreactivity, prove that by chemical analysis this active ingredient is with isolating antitumor all inequality with the anti-hepatitis b active polysaccharide in the past, neutral polysaccharide content is about 52.0%, the content of acidic polysaccharose is about 31.2%, and content of peptides is less than 5%.Heavy metal content wherein is less than 5ppm, and pesticide residue (both " 666, DDT, 5-chloronitrobenzene ") is lower than 10ppb, and the active polysaccharide component of above-mentioned composition was not appeared in the newspapers before the present invention.
Press practice of pharmacy, Ganoderma Applanatum Polysaccharides of the present invention can be prepared into the medicine of various clinical pharmaceutical dosage form as treatment of diseases such as stomach ulcer, acute gastritis, chronic gastritis, duodenal bulbar ulcer and antalgic and inflammation relieving, said medicament is a said formulation on any pharmaceutics.Wherein, oral preparations is selected from any in tablet, capsule, pill, granule, suspensoid, dripping pill, the oral liquid.
Medicine of the present invention preferably contains the Ganoderma Applanatum Polysaccharides of 1%-99% and the vehicle of 99%-1% (medicine that comprises other compatibility), preferably contain the Ganoderma Applanatum Polysaccharides of 30%-80% and the pharmaceutical excipient of 70%-20% (comprising the medicine that other compatibility is used), preferably contain the Ganoderma Applanatum Polysaccharides of 60%-70% and the vehicle of 40%-30% (comprising the medicine that other compatibility is used).
Auxiliary material in the medicine of the present invention is meant conventional vehicle, as solvent, disintegrating agent, correctives, sanitas, tinting material, tackiness agent etc.The medicine that other compatibility in the medicine of the present invention is used, the Ganoderma Applanatum Polysaccharides that refers to effective dose is certain medicine material, again compatibility other can allow the Chinese medicine or the pharmaceutical chemicals that share.
Following pharmacological evaluation has confirmed that the Ganoderma Applanatum Polysaccharides pharmaceutical preparation has pharmacologically actives such as disease treatment such as treatment stomach ulcer, acute gastritis, chronic gastritis, duodenal bulbar ulcer and analgesia:
Animal: Kunming mouse, body weight 18-22g, the male and female dual-purpose, available from the institute for drug control, Jilin Province, the animal quality conformity certification is numbered 10-1008; The Wistar rat, body weight 180-230g, available from Changchun High-technology Medical Animal Experiment Research Center, animal quality conformity certification numbering: 10-5112.
Medicine: the Ganoderma Applanatum Polysaccharides capsule is provided by formulation chamber, the court, is brown ceramic powder, lot number: 20010328, and specification: 0.35g/ grain, 40g crude drug/g powder.INDOMETHACIN is produced by Linfen, Shanxi Province pharmaceutical factory, lot number: 010705, and specification: 25mg/ sheet; WEILEXIN CHONGJI is produced by Pharmaceutical Factory, Bethune Medical Univ., lot number: 011025; Specification: the 5g/ bag, because of its function cures mainly closely with the Ganoderma Applanatum Polysaccharides capsule, and its effective constituent also is polysaccharide (Small acupuncture Hericium erinaceus (Bull. Ex Fr.) Pers. biological fermentation), so be used as the Chinese medicine positive control drug; Cimitidine Type A/AB (cimetidine) is produced by Baiyunshan Pharmaceutical General Factory, lot number: 0109053, and specification: the 0.2g/ sheet, is used as the Western medicine positive control drug by 100 slices/bottle in this test.Acetylsalicylic acid is produced by east, Jilin Province pharmaceutical factory, lot number: 000501, and specification: 25mg/ sheet; Prednisone acetate (prednisone) is produced by Tianjin Pacific Pharmaceutical Co., Ltd., lot number: 010303, and specification: the 5mg/ sheet, respectively as positive control drug; Be made into desired concn with distilled water during the said medicine experiment.
Test materials:
1, bacterial strain
Get mucosa tissue, evenly be applied on the two dish of Skirrow substratum, put 37 ℃ of little oxygen environment (using German biomeerieux Generbox) and add catalyzer, make into little oxygen environment, 5～8%CO2,5～8%O2,33.6%H2:, after hatching 48h,, be accredited as helicobacter pylori through observing bacterium colony, thalli morphology, biochemical reaction and rapid urease test.
The preparation of bacterium liquid: get the platinum erbium adding 5ml brucella broth through the helicobacter pylori inclined-plane of identifying from above-mentioned, culture environment is the same, takes out standby after 48 hours.
2, substratum
Brucella broth, Skirrow substratum
Prepare with the substratum handbook by newly organized bacteriologic test.
3, specimen preparation
Get 5g and add in the 20ml aqua sterilisa, room temperature was soaked after 24 hours, shook up and made the soak solution that every ml is equivalent to the 0.25g sample.
All through " t " check, the result represents with X ± SD statistics.
Experimental technique and result
One, the Ganoderma Applanatum Polysaccharides capsule is to the effect of helicobacter pylori external antibacterial (MIC), sterilization (MBC)
Adopt tube dilution method: get 13 of sterile test tube respectively, every pipe adds brucella broth 1ml, and first test tube adds above-mentioned preparation soup 1ml, shake up, and doubling dilution to the 11 arms, the 12nd, 13 test tube do not add soup, compares test.Each pipe adds the helicobacter pylori bacterium liquid 0.1ml of above-mentioned preparation respectively, about 50-100 bacterium, and mixing is put 37 and ' is cultivated observations behind the 48h in the little oxygen environment of C.Liquid muddiness person is antibiotic, and Clear ﹠ Transparent is responsive, and every pipe is got 1ml simultaneously, adds in two dish, injects the Skirrow substratum, shakes up, and puts and takes out after 37 ℃ of little oxygen are cultivated 48h, and whether observe has bacteria growing.Asepsis growth is for there being germicidal action, otherwise do not have germicidal action.Test-results shows: the Ganoderma Applanatum Polysaccharides capsule does not have antibacterial, germicidal action to external helicobacter pylori.
Two, the Ganoderma Applanatum Polysaccharides capsule is to the influence of stress in rats type ulcer
Get 60 of male and female half and half rats and be divided into 6 groups, every group 10, first group be control group (distilled water, 20ml/kg), irritate stomach cimetidine 1000mg/20ml/kg and the happy new 8000mg/20ml/kg of stomach respectively for second and three group, fourth, fifth, six groups respectively by 800,400 and 200mg/20ml/kg irritate stomach Ganoderma Applanatum Polysaccharides capsule, administration every day secondary, each administration volume is 10ml/kg, successive administration 7 days, after administration in the 6th day, fasting immediately 24 hours, but for drinking-water.After the last administration 2 hours, behind the slight anesthetized rat of ether, the rat four limbs are tied up at iron online, vertically place 16 hours upside down after, sacrificed by decapitation.Behind orifice of the stomach and pylorus ligation, take out full stomach, in stomach, inject physiological saline 10ml, it is immersed in 1% formalin fixes 5 minutes, cut off stomach along greater gastric curvature, with clear water rinsing gently, stomach is lain on the sheet glass, calculate the ulcer grade as follows: complete stomach mucous membrane is 0 grade; Petechial hemorrhage diameter≤1mm, 1～5 is 1 grade; 6～10 is 2 grades; More than 10 be 3 grades; Hemorrhage 1～5 of strip is 4 grades; Article 6～10, be 5 grades; Article 10, more than be 6 grades.The results are shown in Table 1.The Ganoderma Applanatum Polysaccharides capsule has the obvious suppression effect to stress in rats type ulcer.
Table 1 Ganoderma Applanatum Polysaccharides capsule is to the influence of stress in rats type ulcer
Group dosage number of animals ulcer grade inhibiting rate
mg/20ml/kg n ％
Control group-10 5.0 ± 0.471
Cimetidine 1,000 10 0.3 ± 0.483
* *94
Stomach happy new 8,000 10 3.3 ± 0.744
*34
Ganoderma Applanatum Polysaccharides 800 10 1.8 ± 1.398
* *64
400 10 2.4±1.713
*** 52
200 10 3.8±1.033
** 24
Compare with control group:
*P＜0.01,
* *P＜0.001
Three, the Ganoderma Applanatum Polysaccharides capsule is to the influence of rat ligation pylorus type stomach ulcer
Get 60 of rats, grouping and dosage are with experiment one.Administration every day secondary, each administration volume is 10ml/kg, continuous 7 days.The beginning fasting be can't help water 48 hours after administration in the 5th day, then, and under etherization, the ligation stomach pylorus of cutting open the belly continues fasting and stops to supply water sacrificed by decapitation rat after 18 hours after the ligation, open abdomen, the ligation orifice of the stomach takes out stomach, carefully collects gastric juice, and record gastric juice amount, measure the stomach total acid, free acid, pepsin activity.Stomach is opened along big curved scissors, after the rinsing, stomach is lain on the sheet glass gently, check the ulcer grade, see photo, grade scale is as follows: 0 grade: no pathology; 1 grade: hemorrhage, rotten to the corn or take place the ulcer point (＜1mm); 2 grades: 1～5 aphtha (1mm＜2 grade＜3mm); 3 grades: 6 above aphthas or 1 big ulcer (＞3mm); 4 grades: 2 big ulcer (＞3mm); 5 grades: perforated ulcer.More than the results are shown in Table 2.The Ganoderma Applanatum Polysaccharides capsule has the obvious suppression effect to rat ligation pylorus type stomach ulcer, stomach free acid and total acid and peptic activity of stomach is also had the obvious suppression effect, but the gastric juice volume is not had obvious influence.
Table 2 Ganoderma Applanatum Polysaccharides capsule is to the influence of rat ligation pylorus type stomach ulcer
Group dosage stomach ulcer ulcer grade free acid total acidity gastric juice stomach en-gastric juice volume
Mg/kg inhibiting rate mEq/L mEq/L U/L ml/100g
％
Control group-4.8 ± 0.44 40.0 ± 19.9 71.8 ± 16.3 198 ± 55 5.66 ± 1.366
Cimetidine 1,000 70.8 1.4 ± 1.35
* *13.4 ± 14.3
*50.2 ± 15.8
*152 ± 34
*5.20 ± 1.149
Stomach happy new 8,000 29.2 3.4 ± 0.73
* *17.9 ± 21.3
*52.6 ± 20.0
*136 ± 40
*5.36 ± 1.084
Ganoderma Applanatum Polysaccharides 1,000 56.3 2.1 ± 1.36
* *12.4 ± 12.5
*44.0 ± 17.1
*139 ± 57
*5.27 ± 1.113
500 25.0 3.6±0.84
** 15.6±19.3
* 51.9±21.9
* 136±57
* 5.22±1.212
250 20.8 3.9±0.93
* 13.7±14.2
* 47.3±13.7
** 136±42
* 5.05±0.908
Compare with control group:
*P＜0.05,
*P＜0.01,
* *P＜0.001
Four, the Ganoderma Applanatum Polysaccharides capsule is to the therapeutic action of rat acetic acid type stomach ulcer
Get 70 of rats, fasting be can't help water 48 hours, under etherization underwent surgery, and gently was pulled to stomach outside the abdomen, inject 10% acetic acid 0.05ml at body of stomach and pyloric antrum intersection with microsyringe 0.4～0.5mm place under the rat stomach serous coat, the blank group is injected physiological saline 0.05ml.Stomach is sent back in the rat body gently, sew up stomach wall.Operation next day, divide 7 groups with rat is even, all rats press the gastric infusion of dosage shown in the table 3, every day secondary, each administration volume is 10ml/kg, blank group and model control group filling stomach be with volume distilled water, successive administration 14 days.After the last administration 2 hours, the sacrificed by decapitation animal, cut off the abdominal cavity, ligation stomach pylorus and orifice of the stomach are got stomach and were soaked in 1% formalin solution 10 minutes, cut off along greater gastric curvature, stomach is turned up, outwell content, wash out swill with tubule, mucosal surface at injection acetum corresponding site is observed the ulceration degree, calculates ulcer index (average of major diameter of ulcer and minor axis).The results are shown in Table 3.Rounded or the ellipse of ulcer, central concave is swelled all around slightly, and capillary vessel gathers; The ulcer that has healed has protuberance on every side slightly, and the surface is ruddy, and as seen radial microgroove is arranged.The Ganoderma Applanatum Polysaccharides capsule has the obvious suppression effect to rat acetic acid type stomach ulcer.
Table 3 Ganoderma Applanatum Polysaccharides capsule is to the influence of rat acetic acid type stomach ulcer
Group dosage number of animals ulcer index inhibiting rate
mg/20ml/kg n mm ％
Control group-9 0-
Model control group-10 4.35 ± 0.843
Cimetidine 1,000 10 1.15 ± 1.680
* *73.6
Stomach happy new 8,000 9 3.36 ± 1.993 22.8
Ganoderma Applanatum Polysaccharides 1,000 10 1.38 ± 1.542
* *68.4
500 9 2.31±1.948
** 47.0
250 10 3.48±1.865 20.1
Compare with model control group:
*P＜0.01,
* *P＜0.001
Five, the Ganoderma Applanatum Polysaccharides capsule is to the influence of drug-induced type stomach ulcer
Get 70 of rats, be divided into 7 groups at random, press 2 gastric infusions every day of dosage shown in the table 4, each administration volume is 10ml/kg, and blank group and model control group are irritated stomach with volume distilled water, give 7 altogether.Fasting in the 5th day is 48 hours after the administration, can't help water.INDOMETHACIN is dissolved in 5%NaHCO
3In the solution (10mg/1ml), become the suspension of 2mg/1ml again with distilled water diluting, above-mentioned INDOMETHACIN liquid is pressed the dosage of 20mg/kg, once inject to rat back is subcutaneous with half an hour after the administration on the 7th.Rat is put to death in the cervical vertebra dislocation after 6 hours, cut off the abdominal cavity, ligation stomach pylorus and orifice of the stomach, get stomach, in stomach, inject 1% formalin 10ml, will put in order stomach again and be soaked in 1% formalin solution 5-10 minute, with fixing stomach ectonexine, cut off along greater gastric curvature then, rinse well, observe and record gastric mucosa injury situation with tap water.As ulcer index, carry out statistical procedures with the millimeter of ulcer length summation, calculate ulcer and suppress percentage, the evaluation drug effect.The results are shown in Table 4.The Ganoderma Applanatum Polysaccharides capsule has the obvious suppression effect to drug-induced type stomach ulcer.
Table 4, Ganoderma Applanatum Polysaccharides capsule are to the influence of drug-induced type stomach ulcer
Group dosage number of animals ulcer index inhibiting rate
mg/20ml/kg n mm ％
Control group-10 0-
Model control group-10 58.7 ± 14.51
Cimetidine 1,000 10 13.6 ± 13.87
* *76.8
Stomach happy new 8,000 10 27.6 ± 21.10
*53.0
Ganoderma Applanatum Polysaccharides 1,000 10 20.4 ± 13.53
* *65.2
500 10 33.6±16.57
** 42.8
250 10 54.1±20.43 7.8
Compare with model control group:
*P＜0.01,
* *P＜0.001
Six, the influence of rat paw edema due to the Ganoderma Applanatum Polysaccharides capsule on Carrageenan
Get 40 of rats, be divided into 5 groups at random, press secondary every day of dosage shown in the table 5 gastric infusion, each administration volume is 10ml/kg, gives 7 altogether.After the last administration 1 hour, inject 1% carrageenin 0.1ml/ in right back sufficient sole of the foot bottom only, 1,2,3,4,5,6,24 hour foot swelling degree behind the mensuration injection carrageenin, observe medicine and arrive peak times and regression time, relatively the difference condition of administration group and control group the results are shown in Table 5.Rat paw edema has the obvious suppression effect due to the Ganoderma Applanatum Polysaccharides capsule on Carrageenan.
The influence of rat paw edema due to the table 5 Ganoderma Applanatum Polysaccharides capsule on Carrageenan
Group dosage foot swelling degree mm
mg/kg
1h 2h 3h 4h 5h 6h 24h
Control group-5.63 ± 1.51 9.13 ± 2.10 12.13 ± 2.47 10.75 ± 2.43 9.75 ± 1.98 6.50 ± 2.67 2.13 ± 1.55
Prednisone 20 4.75 ± 2.12 8.25 ± 2.05 10.13 ± 2.70 7.88 ± 1.73
*7.86 ± 2.23 6.25 ± 1.28 1.75 ± 1.49
Ganoderma Applanatum Polysaccharides 800 4.00 ± 1.51 7.63 ± 1.92 8.75 ± 1.93
*7.25 ± 2.05
*6.25 ± 2.49
*4.50 ± 2.07 1.13 ± 0.99
400 4.00±1.85 6.13±2.10
* 7.25±2.31
** 7.88±2.64
* 8.00±1.51 7.13±2.36 1.50±1.07
200 4.38±1.30 6.13±1.46
** 8.25±2.12
** 9.13±1.96 8.25±2.60 5.88±1.96 1.75±1.28
Compare with control group:
*P＜0.05,
*P＜0.01
Seven, Ganoderma Applanatum Polysaccharides capsule on Carrageenan causes the influence of mouse foot swelling
Get 50 of mouse, be divided into 5 groups at random by body weight, press secondary every day of dosage shown in the table 6 gastric infusion, each administration volume is 10ml/kg, gives 7 altogether.Half an hour after the last administration, only inject down 1% carrageenin 0.03ml/ in the right back vola aponeurosis (aponeuroses) of mouse, behind the Yu Zhiyan 6 hours, cut the bilateral metapedes, the foot swelling degree is calculated in weighing.The results are shown in Table 6.Ganoderma Applanatum Polysaccharides capsule on Carrageenan causes that the mouse foot swelling has the obvious suppression effect.
Table 6, Ganoderma Applanatum Polysaccharides capsule on Carrageenan cause the influence of mouse foot swelling
Group dosage number of animals ulcer area
mg/20ml/kg n mg
Control group-10 39.8 ± 9.28
Prednisone 20 10 32.4 ± 7.76
Ganoderma Applanatum Polysaccharides 1,200 10 28.8 ± 7.55
*
600 10 32.3±5.58
*
300 10 37.4±8.88
Compare with control group:
*P＜0.05,
*P＜0.01
Eight, the Ganoderma Applanatum Polysaccharides capsule is to the influence of mouse dimethylbenzene auricle oedema
Get 50 of mouse, be divided into 5 groups at random by body weight, press secondary every day of dosage shown in the table 7 gastric infusion, each administration volume is 10ml/kg, gives 7 altogether.After the last administration 30 minutes, 0.05ml dimethylbenzene is evenly dripped the outside in mouse right ear, left ear is the normal control group.After 2 hours, the dislocation of mouse cervical vertebra is put to death, cut two ears, lay round auricle at the same position of left and right ear respectively, weigh, ask the poor of left and right auricle weight, be the swelling degree, the results are shown in Table 7 with the punch tool of diameter 9mm along the auricle baseline.The Ganoderma Applanatum Polysaccharides capsule has the obvious suppression effect to mouse dimethylbenzene auricle oedema.
Table 7, Ganoderma Applanatum Polysaccharides capsule are to the influence of mouse dimethylbenzene auricle oedema
Group dosage number of animals swelling degree
mg/20ml/kg n mg
Control group-10 21.8 ± 6.26
Prednisone 20 10 15.1 ± 4.70
*
Ganoderma Applanatum Polysaccharides 1,200 10 13.2 ± 3.30
*
600 10 15.1±2.81
**
300 10 20.9±6.01
Compare with control group:
*P＜0.05
*P＜0.01
Nine, the Ganoderma Applanatum Polysaccharides capsule is to the analgesic activity (writhing method) of mouse
Get 50 of mouse, female, be divided into 5 groups by body weight, control group feedwater 10mg/kg, all the other each group is pressed the gastric infusion of dosage shown in the table 8 once, and after administration 2 hours, abdominal injection 0.8% acetum 0.1mg/10g, observe the injection back interior mouse of 10min and the writhing response number of times occurs and the writhing response time occurs, the results are shown in Table 7.The Ganoderma Applanatum Polysaccharides capsule has analgesic activity to mouse.
Table 8, Ganoderma Applanatum Polysaccharides capsule are to the analgesic activity (writhing method) of mouse
Group dosage number of animals the body time occurs turning round and turns round the body number of times
Mg/10ml/kg n S time
Control group-10 255 ± 55 14.8 ± 3.61
Asprin 200 10 348 ± 134 8.2 ± 3.61
* *
Ganoderma Applanatum Polysaccharides 1,200 10 352 ± 107
*10.2 ± 5.14
*
600 10 350±139 8.8±6.99
*
300 10 294±69 13.8±3.46
Compare with control group:
*P＜0.05,
* *P＜0.001
Ten, the Ganoderma Applanatum Polysaccharides capsule is to the analgesic activity (hot plate method) of mouse
Get 50 of female mices, be divided into 5 groups (screening earlier before the grouping sufficient person occurs licking for qualified with 5-30 in second), press dosed administration shown in the table 9 once by body weight, after administration 2 hours, to carry out hot plate method and test, the pain thresholding of observing 2 hours mouse the results are shown in Table 9.The Ganoderma Applanatum Polysaccharides capsule has analgesic activity to mouse.
Table 9, Ganoderma Applanatum Polysaccharides capsule are to the analgesic activity (hot plate method) of mouse
Group dosage number of animals the reaction times occurs and licks sufficient number of times
Mg/10ml/kg n S time
Control group-10 20.3 ± 4.00 8.5 ± 2.27
Asprin 200 10 27.5 ± 7.55
*6.0 ± 3.46
Ganoderma Applanatum Polysaccharides 1,200 10 24.6 ± 4.09
*6.1 ± 2.42
*
600 10 22.2±8.56 5.0±1.63
**
300 10 20.0±4.57 7.4±2.41
Compare with control group:
*P＜0.05,
*P＜0.01
Result and discussion
Results of pharmacodynamic test shows that the Ganoderma Applanatum Polysaccharides capsule brings out type stomach ulcer to stress in rats type, ligation stomach pylorus type, acetic acid type and medicine (INDOMETHACIN) restraining effect is all arranged, and its antiulcer action strengthens with dosage.The formed ulcer of coat of the stomach injection acetic acid had the effect that suppresses its development, promotes its healing, the ulcer line of apsides average that high and middle dosage group gastric mucosa of rat surface forms is significantly less than model group, the ulcer healing percentage is respectively 68.4% and 47.0%, when dosage is 250mg/kg (the ulcer healing percentage act as 20.1%), (the ulcer healing percentage act as 22.8% to the happy new 8g/kg of the inhibition strength of Dichlorodiphenyl Acetate type stomach ulcer and stomach, no difference of science of statistics) suitable, promptly the capsular action intensity of Ganoderma Applanatum Polysaccharides is happy new about 32 times an of stomach.To stress ulcer, the ulcer index of three administration treated animals of Ganoderma Applanatum Polysaccharides capsule is starkly lower than model group, ulcer suppresses percentage and is respectively 64% and 52% and 24%, show that the ulcer that this medicine brings out under stressed condition body has certain restraining effect, the happy new 8g/kg effect of the action intensity of Ganoderma Applanatum Polysaccharides capsule 200mg/kg and stomach is close, and promptly the Ganoderma Applanatum Polysaccharides capsule is new about 40 times of stomach pleasure to inhibition strength that stress type stomach ulcer.To ligation pylorus type stomach ulcer, the ulcer index of three administration treated animals of Ganoderma Applanatum Polysaccharides capsule also is starkly lower than model group, ulcer suppresses percentage and is respectively 56.3% and 25.0% and 20.8%, the happy new 8g/kg of the action intensity of Ganoderma Applanatum Polysaccharides capsule 500mg/kg and stomach (it is 29.2% that ulcer suppresses percentage) effect is close, be the Ganoderma Applanatum Polysaccharides capsule to the inhibition strength of ligation pylorus type stomach ulcer be happy new about 16 times of stomach, the Ganoderma Applanatum Polysaccharides capsule does not have obvious influence to the gastric juice volume, but hydrochloric acid in gastric juice and pepsin activity all there are restraining effect, thereby can reduce the erosion and the injury of aggressive factor pair coat of the stomach.And action intensity Ganoderma Applanatum Polysaccharides capsule 500mg/kg is equivalent to the happy new 8g/kg of stomach, promptly is about 16 times of its effect.Medicine (INDOMETHACIN) is brought out type stomach ulcer, the stomach ulcer index of high and middle dosed administration group rat all is starkly lower than model group, ulcer suppresses percentage and is respectively 65.2% and 42.8%, the happy new 8g/kg effect of the action intensity of Ganoderma Applanatum Polysaccharides capsule 1000mg/kg and stomach is close, and promptly the Ganoderma Applanatum Polysaccharides capsule is that stomach is found pleasure in new about 8 times to the inhibition strength that medicine (INDOMETHACIN) brings out type stomach ulcer.It is about 30g that the patients with gastric disease of growing up is taken the happy new dosage of stomach every day, probably needs the dosage about 1.4～5.6g can reach result of treatment and take the Ganoderma Applanatum Polysaccharides capsule.In addition, auxiliary pharmacodynamics test shows that the Ganoderma Applanatum Polysaccharides capsule also has tangible anti-inflammatory and analgesic activity, and this recovery to gastritis and stomach ulcer and colitis etc. also is very favorable.Above result uses clinically for the Ganoderma Applanatum Polysaccharides capsule and has established certain pharmacological basis.
Embodiment
Embodiment 1
Artist's conk 1kg uses decocting 3 times, and three times amount of water is respectively 13,10, the 10ml/g crude drug, each time is 2 hours, filters merging filtrate, be concentrated into relative density 1.03～1.05 (80 ℃), put coldly, add ethanol and be 20% to containing alcohol amount, centrifugal, discard throw out, it is 80% to containing the alcohol amount that supernatant liquor adds ethanol, leaves standstill 24 hours, suction filtration, throw out washs in right amount with dehydrated alcohol, drying, be ground into fine powder, get Ganoderma Applanatum Polysaccharides 25g, yield is 2.5%.
Embodiment 2
With the Chinese medicine artist's conk is raw material, and with 20% alcohol reflux 3 times, three times the ethanol consumption is 6000ml, three times are 3 hours, united extraction liquid, concentrating under reduced pressure, it is 80% to containing the alcohol amount that concentrated solution adds ethanol, left standstill 24 hours, suction filtration, throw out washs in right amount with dehydrated alcohol, dry, be ground into fine powder, get Ganoderma Applanatum Polysaccharides 27g, yield is 2.8%.
Embodiment 3The preparation of tablet
Ganoderma Applanatum Polysaccharides raw material 1000g, medical starch 500g mixes, and uses an amount of alcohol granulation, through the whole grain of pelletizing machine, compressing tablet, every 0.25g, oral, each 1-2 sheet, twice of every day.
Embodiment 4The preparation of capsule
Ganoderma Applanatum Polysaccharides raw material 500g, medical starch 500g mixes, the 0# capsule of packing into, every 0.30g, each oral 1-2 grain, twice of every day.
Embodiment 5The preparation of granule
Ganoderma Applanatum Polysaccharides raw material 100g, starch 1000g, Icing Sugar 400g mixes, and uses an amount of alcohol granulation, and dry, whole grain, packing are promptly.Oral, a 5g, twice on the one.
Claims (3)
1, a kind of Ganoderma Applanatum Polysaccharides, can be by a kind of the preparing in the middle of following two kinds of methods:
(1) be raw material with the Chinese medicine artist's conk, use decocting 1～3 time, each time is 1～3 hour, filter, merging filtrate concentrates, concentrated solution is put cold, adds ethanol and is 20% to containing the alcohol amount, and is centrifugal, discard throw out, it is 80% to containing the alcohol amount that supernatant liquor adds ethanol, leaves standstill 24 hours, suction filtration, throw out washs in right amount with dehydrated alcohol, drying, be ground into fine powder, get Ganoderma Applanatum Polysaccharides;
(2) be raw material with the Chinese medicine artist's conk, with 20% alcohol reflux 3 times, each time is 3 hours, united extraction liquid, it is 80% to containing the alcohol amount that concentrating under reduced pressure, concentrated solution add ethanol, leaves standstill suction filtration 24 hours, throw out washs in right amount with dehydrated alcohol, and drying is ground into fine powder, gets Ganoderma Applanatum Polysaccharides.
2, Ganoderma Applanatum Polysaccharides is being used to prevent and treat stomachache, the hyperchlorhydria disease that stomach ulcer, acute gastritis, chronic gastritis and duodenal bulbar ulcer or other reason cause, and has the application in the medicine of analgesia, anti-inflammatory action.
3, be used for prevention and treatment stomach ulcer, acute gastritis, chronic gastritis and duodenal bulbar ulcer disease, and have the pharmaceutical composition of analgesia, anti-inflammatory action, wherein contain the Ganoderma Applanatum Polysaccharides and the pharmaceutically acceptable carrier for the treatment of significant quantity.
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| CN1332983C (en) * | 2004-12-03 | 2007-08-22 | 中国科学院上海有机化学研究所 | Polysaccharide of rhizoma corydalis, preparation method and application |
| CN103735562B (en) * | 2013-12-06 | 2015-11-18 | 天津植草园生物科技有限公司 | A kind of ganoderma applanatum polysaccharide oral liquid and preparation method thereof |
| CN110590969B (en) * | 2019-10-17 | 2022-06-24 | 吉林农业大学 | Preparation method of uniform acidic Ganoderma Applanatum polysaccharide capable of identifying and regulating lymphocyte |
| CN110655589A (en) * | 2019-10-17 | 2020-01-07 | 吉林农业大学 | A kind of preparation method of Ganoderma lucidum fermented neutral heteropolysaccharide capable of recognizing intestinal cancer cells |
| CN112076281A (en) * | 2020-10-22 | 2020-12-15 | 哈药集团技术中心 | Ganoderma applanatum composition for enhancing immunity and preparation method thereof |
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