CN1204238C - Kenrel oil composite extracted from plant kernel and its extraction process and application - Google Patents
Kenrel oil composite extracted from plant kernel and its extraction process and application Download PDFInfo
- Publication number
- CN1204238C CN1204238C CN 01135911 CN01135911A CN1204238C CN 1204238 C CN1204238 C CN 1204238C CN 01135911 CN01135911 CN 01135911 CN 01135911 A CN01135911 A CN 01135911A CN 1204238 C CN1204238 C CN 1204238C
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- injection
- oil
- semen juglandis
- walnut kernel
- oil composite
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- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a walnut kernel oil composite extracted from walnut kernels, an extraction method thereof and an application thereof in vein emulsion. Every 100 ml of the emulsion of the walnut kernel oil composite comprises the following components: 5 to 30g of walnut kernel oil composite for injection, 0.5 to 3.0g of emulsifier, 0.5 to 3.5g of medium and water for injection as the rest. The walnut kernel oil composite can replenish body's nutrition energy, resist fatigue and hypoxia, obviously enhance body's immunological function, and increase total serum proteins; in addition, the walnut kernel oil composite can inhibit zoograft tumors Lewis and lung cancer, and mouse's liver cancer HAC, tonify the kidneys, warm the lungs, and moisten the intestines; the walnut kernel oil composite has low price. The walnut kernel oil composite is another energy type emulsion with much efficiency.
Description
Technical field
The present invention relates to nut oil composition, extracting method and the application thereof of from plant (wild jujube, Chinese prickly ash or walnut) kernel, extracting.By wherein vein emulsion or other formulation of any nut oil preparation of compositions, be applicable to nutritive deficiency, immunologic hypofunction, all kinds of patients that serum protein is lower replenish the patient of fatty key element etc. as tumour, burn, acquired immune deficiency syndrome (AIDS), infantile malnutrition, surgical postoperative and need.
Background technology
Spina Date Seed or Chinese prickly ash all are the medicine-food two-purpose traditional Chinese medicines, have tonifying liver, the peaceful heart, arrest sweating respectively, promote the production of body fluid and warming middle-JIAO to relieve pain effects such as killing parasites to relieve itching.
Walnut kernel is a kind of medicine-food two-purpose traditional Chinese medicine, has functions such as kidney tonifying, warm lung and ease constipation.But common for a long time walnut oil is a kind of of edible oil, does not see the report of making the outer nutrition agent of intestines and stomach.Because common walnut oil formulation is unfavorable for absorption of human body, and want to make vein breast to the clinical use of people, wherein impurity is many because of extraction and separating technology backwardness cause again, and physics and chemistry values such as acid value do not meet the injection requirement, and walnut oil use pharmaceutically is restricted.
In the prior art, be used for replenishing human nutrition energy, the common high-fat emulsion that has soybean oil, oleum gossypii seminis, safflower wet goods to make with high-fat emulsion.Wherein, using is the high-fat emulsion that soybean oil is made comparatively widely.But the soybean oil fat emulsion mainly works to replenish the human nutrition energy, its effect is more single, relevant enhance immunity function, the rising total serum protein does not appear in the newspapers to the function of the restraining effect of animal transplanting tumor Lewis lung cancer, rat liver cancer HAC and kidney tonifying, warm lung, ease constipation.
Summary of the invention
The technical problem to be solved in the present invention provides provides human nutrition energy, enhance immunity function a kind of can preparing of extracting from plant (wild jujube or Chinese prickly ash or walnut) kernel, have the nut oil composition of the vein breast of new generation of effect such as rising serum protein.
Another technical problem that the present invention will solve provides two kinds of special process methods of extracting above-mentioned plant nut oil composition.
Another technical problem that the present invention will solve is the application of above-mentioned nut oil composition in the preparation vein emulsion.This emulsion can replenish the human nutrition energy, and the enhance immunity function has the effect of rising serum protein, has the function of bulk drug kidney tonifying, warm lung and ease constipation concurrently, and cheap.
Embodiment
First kind of extracting method of nut oil composition of the present invention is as follows, is example with the walnut kernel.
Just carry.Walnut meat powder obtains slightly oil of walnut by squeezing or supercritical extraction method.
Decolouring.Elder generation adds an amount of sherwood oil in the thick oil, stirs, and adds an amount of injection activated carbon again, and insulation is filtered, and reclaims sherwood oil, gets bleached oil.
Alkali refining.Bleached oil and an amount of sherwood oil are dropped into reactor,, calculate the 2%NaOH solution amount, add in the stirring, stir, leave standstill, remove the sub-cloud waste liquid according to the quantity of acid number and bleached oil.Add the warm distilled water of 2 times of amounts in the stirring, stir, static, remove the sub-cloud waste liquid.Use the same method again, time and water temperature carry out the washing second time, sufficient standing removes the sub-cloud waste liquid, the upper strata emulsion.
Breakdown of emulsion.The emulsion layer metering is moved into separating still, by breast layer amount certain proportion, inject required acetone in the stirring, standing demix removes sub-cloud waste water and gets upper oil phase.
Washing.Add through insulation activatory neutral alumina in the required ratio of oil mass and to adsorb, fully stir, leave standstill, filter clear and bright oil; The reactor preheating is dropped in the metering back, adds the process insulation activatory white bole (kaolin) of aequum in the stirring, is incubated vacuum filtration in the stirring; filtrate imports the heating of washing still, removes organic solvent under nitrogen filled protection, injects warm distilled water by the suitable proportion of oil mass; stir, leave standstill, abandon lower floor's waste water; under nitrogen filled protection, the heating oil reservoir dehydrates; clear and bright limpid to oil reservoir, add the activated neutral alumina of aequum, stir; leave standstill, sterile filtration gets refining oil.Nitrogen filled protection mainly is in order to prevent greasy oxidation, to control greasy peroxide value, to keep the quality of oil.
The packing sterilization.Inject respectively in the regulation container, sterilization gets injection Semen Juglandis oil composition.
With the Semen Juglandis oil composition that the inventive method is extracted, have the advantage that good yield height of quality and curative effect are determined, particularly introduced nitrogen filled protection technology, greatly reduce greasy oxidation, improved the quality of composition effectively.
Examples of implementation of aforesaid method.Get the 1000g walnut powder and obtain slightly oil of 450g walnut by the milling process extraction.
Slightly add the sherwood oil of thick oil mass 40% in the oil, stir, add the injection activated carbon of thick oil mass 1% again, 45 ℃ of insulations are after 30 minutes, and filtration, filtrate are reclaimed sherwood oil, get the 428g bleached oil.
Alkali refining.The sherwood oil of bleached oil and oil mass 50% is dropped into reactor, according to the quantity of acid number and bleached oil, add the NaOH solution amount 307ml of 2% concentration, under agitation thread injects the breast layer, continues to stir 10 minutes, leaves standstill 24 hours, removes the sub-cloud waste liquid.Add 45 ℃ of distilled water of 2 times of amounts in the stirring, stir, static 24 hours, remove the sub-cloud waste liquid.Use the same method again, time and water temperature carry out the washing second time, the water yield be 1.5 times that the breast layer is measured, leaves standstill then 48 hours.
Breakdown of emulsion.Remove the sub-cloud waste liquid, get upper strata emulsion 342g and move into separating still, under agitation inject acetone 342ml fast, left standstill 3 hours, remove sub-cloud waste water and get upper oil phase.
Washing.In oil; add through 160 ℃ by 5% of oil mass; insulation activatory neutral alumina adsorbed in 2 hours; and left standstill 30 minutes after fully stirring, filter clear and bright oil, the metering back is dropped into reactor and is preheated to 40 ℃; add while stirring oil mass 3% through 160 ℃; 2 hours insulation activatory white boles (kaolin); and continue to stir, in 50 ℃ of insulations 30 minutes, vacuum filtration; filtrate imports the washing still and is heated to 60 ℃; under nitrogen filled protection, remove organic solvent, inject 45 ℃ distilled water fast, and continue to stir 15 minutes in the ratio of 1 times of oil mass; left standstill then 30 minutes; divide sub-cloud waste water, fill again under the nitrogen state, oil reservoir is heated to 80 ℃ dehydrates in vacuum; clear and bright limpid to oil reservoir; add the activated neutral alumina of aequum, stir, leave standstill; sterile filtration gets refining oil.
The packing sterilization.Inject respectively in the regulation container, sterilization gets 328g injection Semen Juglandis oil composition.
Second kind of nut oil composition extracting method of the present invention is as follows:
1) slightly carry: kernel obtains thick oil by cold press or supercritical extraction, filters, and must clarify crude oil;
2) come unstuck: crude oil is put in the reactor, filled nitrogen, stir, heating adds an amount of phosphoric acid, stirs fast and makes abundant reaction;
3) alkali refining: directly add the equality of temperature NaOH solution for preparing under nitrogen filled protection in the degummed oil mixed solution, the quick stirring fully reacted free fatty acids, heating, fill standing demix under nitrogen, the insulation, removes the sub-cloud soap stock then, gets alkali refining oil;
4) washing: get alkali refining oil, under agitation add an amount of sodium chloride solution, insulation is standing demix down, discards lower floor's waste liquid; Use the same method again, time and water temperature, use purified water washing 2 times instead, standing demix removes the sub-cloud waste liquid, promptly gets and washes oil;
5) dehydration: add an amount of activated alumina in the washing oil, fully stir, leave standstill, filter, must clarify dehydrated oil;
6) decolouring: dehydrated oil is put in the reactor, vacuumized, stir, heat, add proper amount of active carbon and activation kaolin mixture, continue to vacuumize, 80～90 ℃ are stirred down fully, and cooling is filtered, bleached oil;
7) deodorization: bleached oil is put in the stainless steel reaction pot, vacuumize stir with nitrogen under, heating makes oily temperature rise to 140 ℃, stop logical nitrogen and change logical pure water steam, continue to be heated to 190 ℃ and keep constant temperature, logical again steam 1.5 hours, steam off stirs, cools off with nitrogen again, gets deodorised oil;
8) sterilization: deodorised oil is placed the stainless steel sterilization tank, vacuumize, stir, be heated to 160 ℃ and kept constant temperature 2 hours, cooling, sterile filtration is filled nitrogen and is sub-packed in encloses container, promptly gets injection nut oil composition.
Examples of implementation of aforesaid method also are example with the walnut kernel.
1, slightly carries: get the 30kg walnut kernel and obtain slightly oil of walnut kernel, filter, must clarify crude oil 13.1kg by the cold-press extraction.
2, come unstuck: crude oil is put in the reactor, filled nitrogen, stir, heating makes oily temperature rise to 30～35 ℃, adds 13.1g concentration and be 85% phosphoric acid, stirs half an hour fast.
3, alkali refining: under nitrogen filled protection, directly add the equality of temperature 5%NaOH solution that 717g prepares in the degummed oil mixed solution; stirred 30 minutes fast; under stirring at a slow speed, be rapidly heated then to 60～65 ℃; continue to stir at a slow speed 15 minutes; stop to stir; filling standing demix under nitrogen, the insulation, remove the sub-cloud soap stock, get alkali refining oil 12.8kg.
4, washing: get above-mentioned alkali refining oil, under agitation add 1.9kg equality of temperature 0.2% sodium chloride solution, insulation is standing demix down, discards lower floor's waste liquid.Use the same method again, time and water temperature, use distilled water wash instead 2 times, standing demix removes the sub-cloud waste liquid, promptly gets and washes oily 12.6kg.
5, dehydration: add in the above-mentioned washing oil through 2 hours aluminum oxide 1.3kg of 160 ℃ of activation, fully stir, leave standstill more than half an hour, filter, must clarify dehydrated oil 12.0kg.
6, decolouring: above-mentioned dehydrated oil is put in the reactor, vacuumize and be decompressed to 0.082Mpa, stir, be heated to 80～90 ℃, stop to vacuumize, add the 22.5g gac and through 2 hours 337.5g kaolin mixture of 160 ℃ of activation, continue to vacuumize being decompressed to 0.082Mpa, 80～90 ℃ are continued down to stir 20 minutes, are cooled to 40 ℃, filter, get bleached oil 11.0kg.
7, deodorization: above-mentioned bleached oil is put in the stainless steel reaction pot, vacuumize and be decompressed to 0.082Mpa, logical simultaneously nitrogen stirs, heating makes oily temperature rise to 140 ℃, stops logical nitrogen and changes logical pure water steam, continues to be heated to 190 ℃ and keep constant temperature, logical again steam 1.5 hours, steam off stirs, cools off with nitrogen again, gets deodorised oil 10.6kg.
8, sterilization packing: deodorised oil is placed the stainless steel sterilization tank, vacuumizes and be decompressed to 0.082Mpa, stirring, be heated to 160 ℃ and kept constant temperature 2 hours, cooling, sterile filtration is filled nitrogen and is sub-packed in encloses container, injection Semen Juglandis oil composition 9.6kg.
Spina Date Seed of the present invention or Semen Pericarpium Zanthoxyli or Semen Juglandis oil composition are a kind of faint yellow transparent oily liquids, and its physicochemical constant is: relative density 0.920～0.930; Refractive index 1.470～1.480; Acid number＜0.80 (mgKOHg
-1); Iodine number 120.0～155.0; Saponification value 180.0.～200.0; Peroxide value＜30.0 (meqkg
-1) residue on ignition 0.01～0.04%; Arsenic salt＜0.4ppm; Heavy metal＜2ppm; Molecular-weight average 873.969.The component of Semen Juglandis oil composition comprises triglyceride level 95.915 ± 0.794%, 1,3 triglyceride, 1.328 ± 0.516%, 1,2 triglyceride 0.899 ± 0.276%, Sitosterol 0.313 ± 0.069%.Fatty acid component after the esterlysis is hexadecanoic acid 6.26 ± 0.62%, octadecanoic acid 2.31 ± 0.35%, 18 carbon monoenoic acids 21.88 ± 1.93%, octadecadienoic acid 59.98 ± 1.52%, punicic acid 9.50 ± 1.43%.
Great advantage with second method is not with an organic solvent, thereby has avoided pollution.Peroxide value in the nut oil composition that this method is extracted can＜6.0 (meqkg
-1).
The application of Semen Juglandis oil composition in the preparation Liposyn.An amount of emulsifying agent and an amount of isotonic agent are added aqueous phase, under certain speed, after the stirring and dissolving, add the injection Semen Juglandis oil composition of significant quantity, add the injection water and make colostrum to 100ml, and with NaOH solution adjusting colostrum pH value to 8.5.Colostrum is transferred in the homogenize bucket then, carried out homogenize, homogenize pressure: low pressure 10～20Mpa, high pressure 45～60Mpa with homogenizer; The homogenize temperature is 50～80 ℃, and by above-mentioned homogenize condition homogenize 6 times, emulsion promptly gets the Semen Juglandis oil high-fat emulsion through filtration, can, sterilization.The each component consumption is injection Semen Juglandis oil composition 5～30g; Injection phosphatide 1.0～3.0g; Glycerol for injection 1.5～3.0g; Water for injection adds to 100ml.
Emulsifying agent of the present invention can be phosphatide (comprising soybean lecithin, Ovum Gallus domesticus Flavus lecithin, soybean phospholipid), Pu Lulang Garrick (Pluronic), Polyglycerine palmitinic acid glycol etc.; Isotonic agent can be glycerine, sorbyl alcohol, Xylitol, glucose etc.
Usually injection Semen Juglandis oil composition is got 10～30g; When making vein emulsion, more safe and reliable with naturally occurring emulsifying agent such as soybean (or yolk) Yelkin TTS, consumption is 1.0～2.0g; Isotonic agent adopts glycerine, and consumption is 1.5～3.0g; Water for injection adds to 100ml.
Oral capsule formulation technology of the present invention is as follows.One batching takes by weighing vitamin-E 0.675g (antioxidant), at room temperature throws in 900g Semen Ziziphi Spinosae oil or pericarpium zanthoxylum oil or Semen Juglandis oil, and stirring and dissolving becomes a kind of clarification, clear solution.Two colloidal sols, in gelatin: water: glycerine: sanitas=1: 1: 0.4: 0.001 ratio, weigh up a certain amount of rubber raw material, wherein the gelatin quality meets under two P388 items of Chinese Pharmacopoeia (95 editions), qualities of glycerin meets under two P78 items of Chinese Pharmacopoeia (95 editions), sanitas adopts ethyl p-hydroxybenzoate, belongs to ester class sanitas; Most of distilled water is dropped in the reactor, be heated to 50～60 ℃, open and stir, drop into gelatin, glycerine and sanitas etc., container is washed with residue distillation pond, pours in the pot, heats up gradually, makes the material off-bottom, evenly; The off-response pot is opened vacuum pump, and pot interior vacuum tightness is maintained between gauge pressure 0.065～0.080Mpa, keeps 30～60 minutes, removes vacuum, and feed liquid is filtered in the charging basket through 120 mesh sieves, in 50～60 ℃ of heat preservation for standby use.Three compactings, under the processing condition of 21～24 ℃ of room temps, relative humidity＜40%, 50～60 ℃ of glue box temperature, dispenser voltage 110～150V, 10～15 ℃ of adhesive tape temperature, rubber thickness 0.8～1.0mm, suppress 1000 soft capsules with No. 8 moulds, regularly detect the loading amount of content.Capsule and pill, is wiped ball and is deoiled after 4 hours in pressed sizing, changes baking just 8 hours over to, and after sherwood oil (30～50 ℃) washing, oven dry is packed after useless ball is chosen in arrangement.
Semen Juglandis oil vein emulsion of the present invention, emulsifying agent is safe and reliable, prescription is reasonable.Except replenishing the body nutrient energy, antifatigue, hypoxia tolerance, significantly improve immune function of human body, have outside the effect of rising total serum protein, it also has certain restraining effect to animal transplanting tumor Lewis lung cancer, rat liver cancer HAC, and have the function of bulk drug kidney tonifying, warm lung and ease constipation concurrently, and cheap, be another energy type emulsion with many effects.
Emulsion of the present invention shows that through animal acute toxicity experiment the maximum tolerated dose of mouse vein administration is 75ml/kg/ day, and untoward reaction and death condition do not appear in the experimental session mouse; Dissect back pluck organ no abnormality seen.
Medicine haemolysis method test routinely, the result does not have hemolytic action.By the cavy systemic allergy test is shown that emulsion of the present invention does not have tangible anaphylaxis to cavy.
Rabbit auricular vein (blood vessel) was slowly injected emulsion 15ml/kg of the present invention and 5ml/kg dosage respectively in continuous 7 days, and the result shows does not have tangible hormesis to the rabbit auricular vein.
Record the energy calorific value by GB384-81, Semen Juglandis oil composition of the present invention is that 37599kj/kg is suitable with soybean oil 37243kj/kg, and Semen Juglandis oil emulsion is 6303kj/kg.
Semen Juglandis oil emulsion is to the method and the result of mouse hypoxia tolerance, anti-fatigue test.Select 50 of healthy male mices, be divided into 5 groups at random, 10 every group by body weight.Three dosage groups of Semen Juglandis oil emulsion are respectively 25ml/kg, 12.5ml/kg, 6.25ml/kg, and other establishes the physiological saline group and gives physiological saline with volume.Intravenous injection every day once, successive administration 7 days was tested after the last administration in 2 hours.
Hypoxia endurance test: will respectively be tried mouse and put into the airtight wide-necked bottle of 250ml water white transparency respectively, and put into 10 gram sodica calxs in each bottle, and put into bottle certainly and pick up counting, and cease breathing until mouse.Calculate mean survival time (min), relatively the significance of difference between each administration group and the physiological saline group.
Anti-fatigue test: mouse is weighed respectively, bear a heavy burden in mouse root of the tail portion by 5% of body weight, put into 25 ℃ of water temperatures, in the big water vat of depth of water 40cm, it is dead until sinking under water to force mouse to continue swimming, write down each mouse and continue swimming time (min), carry out the t check, relatively the significance of difference between each administration group and the physiological saline group.
Table 1, Semen Juglandis oil emulsion various dose intravenously administrable is to the influence of mouse hypoxia tolerance, antifatigue
Hypoxia tolerance under the swimming with a load attached to the body test normal pressure
Grouping (min) rate elongation survival time dosage time length (min) rate elongation
(ml/kg) ( x±SD) (％) ( x±SD) (％)
Physiological saline 9.18 ± 5.0 30.46 ± 5.33
6.25 18.13±16.64 97.58 34.91±7.59 14.61
Semen Juglandis oil
Emulsion 12.5 19.96 ± 9.65
*17.56 41.77 ± 9.38
*37.13
25 22.88±7.89
** 149.31 43.73±5.37
** 43.56
Compare with the physiological saline group,
*P＜0.05,
*P＜0.01.
Two testing datas see the above table 1.The hypoxia endurance test statistical treatment shows, the mouse mean survival time difference highly significant (P＜0.01) of the mouse mean survival time of the middle and high dosage group of Semen Juglandis oil emulsion and physiological saline group.The test-results prompting, sample Semen Juglandis oil emulsion has certain resisting oxygen lack to mouse, the non-specific resistivity of energy enhancing body, dose-effect relationship is obvious.
Swimming with a load attached to the body test statistics analysis revealed, the mouse of the middle and high dosage group of Semen Juglandis oil emulsion continues swimming time and physiological saline group comparing difference highly significant (P＜0.01).Above-mentioned two test-results prove that Semen Juglandis oil emulsion can obviously prolong the swimming time of mouse under the effect of 25ml/kg dosage, strengthen the mouse anti-reflecting fatigue effect, and dose-effect relationship is obvious.
Semen Juglandis oil emulsion is to the test method of immune function of mice influence.30 of C57BL/6 are divided into 4 groups at random, and 6 every group, i.e. Semen Juglandis oil emulsion 25ml/kg, 12.5ml/kg, 6.25ml/kg, physiological saline group (0.5ml/ is only), all vi * 7.Administration finishes the back and puts to death animal, gets spleen under the aseptic condition.
Lymphocyte increment influence test: the counting splenocyte, and the adjustment cell concn is 1 * 10
7Individual/ml, every hole adds cell suspension 100ul on 96 well culture plates, ConA 50ul and nutrient solution, and each group is all established three multiple holes, 37 ℃, 5%CO
2Cultivated 48 hours under the condition, add
3H-TdR 0.5 μ ci/ hole continues to cultivate 18 hours.With bull cell harvestor collecting cell, on liquid scintillation instrument, survey the CPM value, and compare with control group.
Table 2, Semen Juglandis oil emulsion various dose intravenously administrable is to effect of immunologic function
Group dosage lymphocyte increment IL-2 NK activity
(ml/kg) (x±SD) (x±SD) Pi(％)
Physiological saline 25 6049 ± 459 750 ± 112
Intralipid 12.5 6248±209 751±96
* 1.2
6.25 8913±911
** 941±103
** 27.9
Semen Juglandis oil emulsion 12.5 8988 ± 1374
*875 ± 160
*43.8
25.0 8480±1283
** 917±30
** 31.9
*P＞0.5, Intralipid (soybean oil fat breast) compares with physiological saline.
*P＜0.01, Semen Juglandis oil emulsion and physiological saline group and Intralipid vein emulsion group are relatively.
Above-mentioned Semen Juglandis oil emulsion medical effect experiment shows:, during 6.25ml/kg dosage, the effect of obvious promotion mice spleen lymphocytes proliferation is arranged all 25,12.5; Continuous intravenous injection can activate natural killer cell (NK) activity after 7 days; The effect that each dosage group intravenous injection all had promotion interleukin-2 (IL-2) to generate in 7 days.Above-mentioned experiment shows that emulsion of the present invention, has than the better effect of soybean oil fat breast as the aspects such as generation of body spleen lymphocyte proliferation, raising NK activity and promotion IL-2 in the enhance immunity function.
Table 3, Semen Juglandis oil emulsion is to the influence of mice serum total protein
Group dosage total serum protein (g/L)
(ml/kg) (x±SD)
Physiological saline 10 69.8 ± 8.7
2.5 85.4±14.0
**
Semen Juglandis oil emulsion 5 86.1 ± 10.4
*
10 79.8±7.4
*
Compare with the physiological saline group,
*P＜0.05,
*P＜0.01.
Table 4,7 days restraining effect of Semen Juglandis oil emulsion various dose vein successive administration to rat liver cancer HAC
Heavy (g) tumour inhibiting rate of group dosage number of animals the weight of animals (g) knurl
(ml/kg) all the time (x ± SD) (%)
Physiological saline 10 10 10 19.2 26.5 2.48 ± 0.31
6.25 10 10 19.1 25.5 1.92±0.35
** 22.58
Semen Juglandis oil emulsion 12.5 10 10 19.4 24.9 1.83 ± 0.39
*26.21
25 10 10 19.4 24.5 1.45±0.22
** 41.53
Compare with the physiological saline group,
*P＜0.01.
Table 5, Semen Juglandis oil emulsion various dose vein successive administration 7 days is to the Mice Bearing Lewis Lung Cancer restraining effect
Heavy (g) tumour inhibiting rate of group dosage number of animals the weight of animals (g) knurl
(ml/kg) all the time (x ± SD) (%)
Physiological saline 25 66 18.5 24.4 2.25 ± 0.30
6.25 6 6 18.1 23.5 1.87±0.39 17.04
Semen Juglandis oil emulsion 12.5 66 18.6 22.9 1.63 ± 0.40
*27.41
25 6 6 18.4 22.5 1.27±0.18
** 43.70
Compare with the physiological saline group,
*P＜0.01.
From table 4 table 5 as seen, Semen Juglandis oil emulsion has the effect of certain inhibition rat liver cancer HAC and Lewis lung cancer growth when above-mentioned dosage.
Further discover,, use the made emulsion of the Semen Juglandis oil composition that from walnut kernel, extracts in, also find to have above-mentioned similar function as the energy breast because walnut kernel has the effect of kidney tonifying, warm lung and ease constipation.
Embodiment 1, and soybean lecithin 1.2g and glycerine 2.5g are added water 25ml, after stirring and dissolving under 5000 rev/mins of speed, adds injection Semen Juglandis oil composition 20g, adds water to 100ml and makes colostrum, and regulate the colostrum pH value to 6-9 with NaOH solution.Colostrum is transferred in the homogenize bucket then, carried out homogenize, homogenize pressure: low pressure 10～20Mpa, high pressure 40-～60Mpa with homogenizer; The homogenize temperature is 60～80 ℃, and by above-mentioned homogenize condition homogenize 6 times, emulsion promptly gets 20% for vein Semen Juglandis oil high-fat emulsion through filtration, can, sterilization.
Embodiment 2, and Ovum Gallus domesticus Flavus lecithin 1.0g and glycerine 1.5g are added water 20ml, after stirring and dissolving under the same speed, add injection Semen Juglandis oil composition 10g, add water to 100ml and make colostrum.Promptly make 10% Semen Juglandis oil high-fat emulsion through toning pH value, homogenize, filtration, can, sterilization again.
Embodiment 3, and soybean phospholipid 2.0g and glycerine 3.0g are added water 30ml, after stirring and dissolving under the same speed, add injection Semen Juglandis oil composition 30g, add water to 100ml and make colostrum.Promptly make 30% Semen Juglandis oil high-fat emulsion through toning pH value, homogenize, filtration, can, sterilization again.
The Semen Juglandis oil composition that extracts with the present invention also can be prepared into other formulation such as oral breast, soft capsule, iodipin etc. except the newborn main ingredient of vein that human nutrition energy etc. is provided.
Claims (7)
1, a kind of Semen Juglandis oil composition that from walnut kernel, extracts by squeezing or supercritical extraction, it is characterized in that said composition contains triglyceride level 95.915 ± 0.794%, 1,3 triglycerides 1.328 ± 0.516%, 1,2 triglycerides 0.899 ± 0.276% and Sitosterol 0.313 ± 0.069%, the lipid acid after the said composition esterlysis consist of hexadecanoic acid 6.26 ± 0.62%, octadecanoic acid 2.31 ± 0.35%, 18 carbon monoenoic acids 21.88 ± 1.93%, octadecadienoic acid 59.98 ± 1.52% and punicic acid 9.50 ± 1.43%.
2, the Semen Juglandis oil composition that extracts from walnut kernel according to claim 1 is characterized in that: said composition is pressed fatty oil and is detected, and its physicochemical constant is: relative density 0.920～0.930; Refractive index 1.470～1.480; Acid number＜0.80mgKOHg
-1Iodine number 120.0～155.0; Saponification value 180.0.～200.0; Peroxide value＜30.0meqkg
-1Residue on ignition 0.01～0.04%; Arsenic salt＜0.4ppm; Heavy metal＜2ppm; Molecular-weight average 873.969.
3, a kind of pharmaceutical preparation with antifatigue or hypoxia tolerance or antitumor action is characterized in that containing the claim 1 or the 2 described Semen Juglandis oil compositions of significant quantity.
4, pharmaceutical preparation according to claim 3 is characterized in that said preparation is lipomul or capsule.
5, pharmaceutical preparation according to claim 4 is characterized in that also containing emulsifying agent, isotonic agent and water for injection.
6, pharmaceutical preparation according to claim 5 is characterized in that the each component consumption is:
Injection Semen Juglandis oil composition 5～30g;
Injection phosphatide 1.0～3.0g;
Glycerol for injection 1.5～3.0g;
Water for injection adds to 100ml.
7, pharmaceutical preparation according to claim 6 is characterized in that the each component consumption is:
Injection Semen Juglandis oil composition 20g;
Soybean lecithin for injection 1.2g;
Glycerol for injection 2.5g;
Water for injection adds to 100ml.
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| US20060233863A1 (en) | 2003-02-10 | 2006-10-19 | Enzymotec Ltd. | Oils enriched with diacylglycerols and phytosterol esters and unit dosage forms thereof for use in therapy |
| IL154381A0 (en) * | 2003-02-10 | 2003-09-17 | Enzymotec Ltd | A composition for reducing blood cholesterol and triglycerides |
| EP1488785A1 (en) * | 2003-06-18 | 2004-12-22 | B. Braun Melsungen Ag | Oil emulsion for postnatal substitution of hormones |
| JP5118965B2 (en) | 2004-08-10 | 2013-01-16 | エンジモテック リミテッド | Treatment methods that require plant components |
| CN100358990C (en) * | 2005-04-26 | 2008-01-02 | 中国科学院沈阳应用生态研究所 | A kind of aqueous extraction method of pecan oil |
| CN102250686A (en) * | 2011-06-16 | 2011-11-23 | 湖南盈成油脂工业有限公司 | Oil degumming method |
| FR2984748B1 (en) * | 2011-12-26 | 2017-02-10 | Expanscience Lab | USE OF WHOLE WHOLE LAWYERS TO OBTAIN AN INAPPENSIBLE AVOCADO OIL |
| CN104012681B (en) * | 2013-03-29 | 2015-11-18 | 洛阳春魁农业开发有限公司 | A kind of peony seeds ready-mixed oil containing spine date seed oil and preparation method thereof |
| CN105419398A (en) * | 2015-12-19 | 2016-03-23 | 仇颖超 | Preparation method of Chinese prickly ash seed fatty acid modified heavy calcium carbonate |
| CN107057845A (en) * | 2017-03-17 | 2017-08-18 | 湖北旭舟林农科技有限公司 | A kind of method of idesia oil extraction purification |
| CN107099368A (en) * | 2017-04-10 | 2017-08-29 | 浙江海洋大学 | High EPA, DHA content a kind of swimming crab crab oil oil producing method |
-
2001
- 2001-10-17 CN CN 01135911 patent/CN1204238C/en not_active Expired - Lifetime
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