CN120232810A - Optical measurement systems, sample analyzers - Google Patents

Abstract

The application discloses an optical measurement system and a sample analyzer, wherein a sheath flow component of the optical measurement system is used for enabling a sample to be measured to pass through a detection area along a first direction, a laser component is used for emitting laser to the detection area so as to enable particles to be measured in the detection area to emit fluorescence, and a fluorescence detection component is used for receiving the fluorescence excited by the particles to be measured irradiated by the laser. Through the mode, the laser components emit lasers with different wavelengths, so that particles to be detected respectively excite first fluorescence and second fluorescence, the fluorescence detection component comprises a first fluorescence receiver for receiving the first fluorescence and a second fluorescence receiver for receiving the second fluorescence, the first fluorescence receiver and the second fluorescence receiver are arranged at intervals along a first direction, the crosstalk degree between the first fluorescence and the second fluorescence is reduced, the fluorescence collection performance of the first fluorescence receiver and the second fluorescence receiver is improved, and the measurement accuracy of the optical measurement system is further improved.

Description

Optical measurement system and sample analyzer

Technical Field

The application relates to the technical field of sheath flow detection, in particular to an optical measurement system and a sample analyzer.

Background

When the existing optical measurement system uses a plurality of laser beams with different wavelengths to carry out fluorescence detection, the laser beams with different wavelengths can generate light deflection with different degrees after being refracted by the same lens, so that the fluorescence beams with different wavelengths excited by the sheath flow component are easy to cross each other, and the accuracy of optical measurement is affected.

Disclosure of Invention

In order to solve the technical problems, the application provides an optical measurement system and a sample analyzer.

In order to solve the above problems, the present application provides an optical measurement system, which includes a sheath flow assembly, a laser assembly and a fluorescence detection assembly. The sheath flow assembly comprises a detection area, the detection area is used for passing through a sample flow to be detected along a first direction, the sample flow to be detected comprises a plurality of particles to be detected, the laser assembly is arranged on one side of the sheath flow assembly and used for emitting laser to the detection area so as to enable the particles to be detected in the detection area to emit fluorescence, the fluorescence detection assembly is arranged on one side of the sheath flow assembly away from the laser assembly and used for receiving the fluorescence emitted by the particles to be detected through the laser irradiation, the laser assembly is used for emitting lasers with different wavelengths so as to enable the particles to be detected to emit first fluorescence and second fluorescence respectively, the fluorescence detection assembly comprises a first fluorescence receiver used for receiving the first fluorescence and a second fluorescence receiver used for receiving the second fluorescence, and the first fluorescence receiver and the second fluorescence receiver are arranged at intervals along the first direction.

Optionally, the optical measurement system further comprises a processor, wherein the processor is connected with the fluorescence detection component, and the processor is used for adjusting the light intensity parameters received by the first fluorescence receiver and the second fluorescence receiver based on the time difference of the excitation of the first fluorescence and the second fluorescence by the laser component so as to obtain the detection result of the sample flow to be detected.

Optionally, the first fluorescence receiver is used for converting the optical signal of the first fluorescence into a first light intensity parameter, the second fluorescence receiver is used for converting the optical signal of the second fluorescence into a second light intensity parameter, the processor is further used for receiving the first light intensity parameter and the second light intensity parameter from the first fluorescence receiver and the second fluorescence receiver at a preset sampling frequency, delaying at least one of the first light intensity parameter and the second light intensity parameter according to sampling points, wherein the sampling points are obtained based on the sampling frequency and the time difference, and obtaining the detection result based on the first light intensity parameter and the second light intensity parameter after delay processing.

Optionally, the laser assembly is configured to emit a first laser and a second laser with different wavelengths, where a beam of the first laser and a beam of the second laser irradiated on the detection area have a first height difference, and the processor is configured to calculate the time difference based on the first height difference and a flow velocity of the sample flow to be detected.

Optionally, the fluorescence detection assembly further includes a light receiving device disposed between the sheath flow assembly and the fluorescence detection assembly, the light receiving device is configured to amplify and collect a fluorescence beam excited by the sheath flow assembly, the first fluorescence forms a light spot of a first size on a light receiving surface of the first fluorescence receiver, the second fluorescence forms a light spot of a second size on a light receiving surface of the second fluorescence receiver, and a sum of the first size and the second size is less than or equal to 2 times of a product of the first height difference, an amplification factor of the light receiving device, and a flow velocity of the sample flow to be measured.

Optionally, the first fluorescence receiver and the second fluorescence receiver are staggered and have a second height difference in the first direction, and the second height difference is greater than or equal to half of a sum value of the spot sizes of the first fluorescence receiver and the second fluorescence receiver.

Optionally, the fluorescence detection assembly further comprises a third fluorescence receiver, wherein the laser assembly is used for emitting laser with different wavelengths so as to enable the particles to be detected to excite third fluorescence, the first fluorescence receiver, the second fluorescence receiver and the third fluorescence receiver are arranged in a staggered mode, the second fluorescence receiver and the third fluorescence receiver have a third height difference in the first direction, and the third height difference is larger than or equal to half of the sum value of the spot sizes of the second fluorescence receiver and the third fluorescence receiver.

Optionally, the optical measurement system further comprises a processor, the processor is connected with the fluorescence detection assembly, the particles to be measured comprise magnetic beads and first fluorescent substances, the magnetic beads are coated with second fluorescent substances with different intensity levels, the first fluorescent substances are used for quantitative analysis, the second fluorescent substances are used for classified analysis, different intensity levels correspond to different classified detection items, and the processor is used for adjusting light intensity parameters received by the first fluorescent receiver and the second fluorescent receiver based on time difference of exciting the first fluorescent light and the second fluorescent light by the laser assembly so as to output quantitative information respectively corresponding to each classified detection item of the sample to be measured.

Optionally, the magnetic beads are coated with a second fluorescent material and a third fluorescent material with different intensity levels, the fluorescent detection assembly further comprises a third fluorescent receiver, the second fluorescent material and the third fluorescent material are irradiated by the same laser of the laser assembly, so that the second fluorescent receiver receives fluorescence excited by the second fluorescent material, and the third fluorescent receiver receives fluorescence excited by the third fluorescent material, wherein the second fluorescent receiver and the third fluorescent receiver are arranged in parallel in the vertical direction of the first direction.

The application provides an optical measurement system and a sample analyzer, wherein a sheath flow component of the optical measurement system is used for enabling a sample to be measured to pass through a detection area along a first direction, a laser component is used for emitting laser to the detection area so as to enable particles to be measured in the detection area to emit fluorescence, and a fluorescence detection component is used for receiving the fluorescence excited by the particles to be measured irradiated by the laser. Through the mode, the laser components emit lasers with different wavelengths, so that particles to be detected respectively excite first fluorescence and second fluorescence, the fluorescence detection component comprises a first fluorescence receiver for receiving the first fluorescence and a second fluorescence receiver for receiving the second fluorescence, the first fluorescence receiver and the second fluorescence receiver are arranged at intervals along a first direction, the crosstalk degree between the first fluorescence and the second fluorescence is reduced, the fluorescence collection performance of the first fluorescence receiver and the second fluorescence receiver is improved, and the measurement accuracy of the optical measurement system is further improved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:

FIG. 1 is a schematic diagram of an optical measurement system according to an embodiment of the present application;

FIG. 2 is a schematic diagram of another embodiment of an optical measurement system provided by the present application;

fig. 3 is a schematic structural view of another embodiment of the optical measurement system provided by the present application.

Detailed Description

The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art without making any inventive effort, are intended to be within the scope of the present application.

It should be noted that, if directional indications (such as up, down, left, right, front, and rear are referred to in the embodiments of the present application), the directional indications are merely used to explain the relative positional relationship, movement conditions, and the like between the components in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indications are correspondingly changed.

In addition, if there is a description of "first", "second", etc. in the embodiments of the present application, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present application.

An optical measurement system is provided in the embodiment of the present application, please refer to fig. 1, fig. 1 is a schematic structural diagram of an embodiment of the optical measurement system provided in the present application. As shown in fig. 1, the optical measurement system includes a sheath flow assembly 10, a laser assembly (not shown), and a fluorescence detection assembly 30.

The sheath flow assembly 10 comprises a detection area for passing through a sample flow to be detected along a first direction, wherein the sample flow to be detected comprises a plurality of particles to be detected, the laser assembly is arranged on one side of the sheath flow assembly 10 and is used for emitting laser to the detection area so as to enable the particles to be detected in the detection area to emit fluorescence, the fluorescence detection assembly 30 is arranged on one side of the sheath flow assembly 10 away from the laser assembly, and the fluorescence detection assembly 30 is used for receiving the fluorescence excited by the particles to be detected irradiated by the laser. Specifically, the sample flow to be measured flows through the detection area along the first direction, and the laser component emits laser along the detection area along the direction perpendicular to the first direction. The sheath flow assembly 10 may include a flow chamber and a nozzle for ejecting a sample stream to be tested toward the detection zone such that the sample stream to be tested flows through the detection zone as individual particles to be tested. In the flowing process of the sample flow to be detected, the laser component emits laser to the detection area so that the particles to be detected in the detection area are excited to emit fluorescence and scattered light under the irradiation of the laser, and the optical measurement system acquires the detection result of the sample flow to be detected by collecting the excited fluorescence and the scattered light.

The laser component is used for emitting laser with different wavelengths so that the particles to be detected respectively excite first fluorescence and second fluorescence. The laser assembly may include at least a first laser configured to emit a first laser and a second laser configured to emit a second laser, where the positions of the light beams irradiated at the detection area by the first laser and the second laser do not coincide. When the sample flow to be measured flows in the detection area, laser delay is arranged between the first laser and the second laser, the sample flow to be measured flows in the detection area according to a preset flow speed, the first laser irradiates the particle to be measured of the sample flow to be measured on the first position and acquires light intensity data of the particle to be measured corresponding to the first fluorescence, the particle to be measured flows to the second position after the time of laser delay and receives irradiation of the second laser, so that the light intensity data of the particle to be measured corresponding to the second fluorescence is acquired, and the optical measurement system can integrate different light intensity data of the same particle to be measured through the first laser and the second laser.

The fluorescence detection assembly 30 includes a first fluorescence receiver 310 for receiving a first fluorescence and a second fluorescence receiver 320 for receiving a second fluorescence, the first fluorescence receiver 310 and the second fluorescence receiver 320 being spaced apart along a first direction. It will be appreciated that the wavelength bands of the first fluorescence and the second fluorescence are different, and the optical measurement system allows the first fluorescence to be projected to the first fluorescence receiver 310 in the vertical direction of the first direction and the second fluorescence to be projected to the second fluorescence receiver 320 in the vertical direction of the first direction without crosstalk by providing the first fluorescence receiver 310 and the second fluorescence receiver 320 at different height positions of the first direction.

In the embodiment of the present application, the sheath flow assembly 10 of the optical measurement system is used for allowing the sample to be measured to pass through the detection area along the first direction, the laser assembly is used for emitting laser to the detection area so as to make the particles to be measured in the detection area emit fluorescence, and the fluorescence detection assembly 30 is used for receiving the fluorescence emitted by the laser irradiating the particles to be measured. Through the above manner, the laser components emit laser with different wavelengths, so that the particles to be measured respectively excite first fluorescence and second fluorescence, the fluorescence detection component 30 comprises a first fluorescence receiver 310 for receiving the first fluorescence and a second fluorescence receiver 320 for receiving the second fluorescence, the first fluorescence receiver 310 and the second fluorescence receiver 320 are arranged at intervals along the first direction, the crosstalk degree between the first fluorescence and the second fluorescence is reduced, the fluorescence collection performance of the first fluorescence receiver 310 and the second fluorescence receiver 320 is improved, and the measurement accuracy of the optical measurement system is further improved.

Wherein at least one of the first fluorescence receiver 310 and the second fluorescence receiver 320 of the present embodiment may be used for performing a classification analysis of a sample flow to be measured, for example, the first fluorescence receiver 310 and the second fluorescence receiver 320 are used for performing a classification analysis, or one of the first fluorescence receiver 310 and the second fluorescence receiver 320 is used for performing a classification analysis, and the other of the first fluorescence receiver 310 and the second fluorescence receiver 320 is used for performing a quantitative analysis.

Each of the test particles is coupled with at least one test substance, wherein the quantitative analysis may be for analyzing the concentration of the test substance in the sample stream to be tested. The substances to be measured can be various antigens or antibodies in the blood sample, such as antigen A/B/C/D/and the like, or other substances needing to be classified and counted, each substance to be measured corresponds to a classified detection item, and classified analysis can be used for analyzing the types of different substances to be measured in the sample flow to be measured. The detection result output by the processor comprises quantitative or concentration information corresponding to each classified detection item.

In one embodiment, the optical measurement system further includes a processor (not shown) coupled to the fluorescence detection assembly 30. The processor is configured to adjust the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on the time difference between the excitation of the first fluorescence and the second fluorescence by the laser component, so as to obtain a detection result of the sample flow to be detected.

Specifically, in the optical measurement system of this embodiment, when the sample flow to be measured in the detection area is irradiated with the lasers with different wavelengths, there is a laser delay in the time when the laser component excites the first fluorescence and the second fluorescence, so that there is a time difference between when the laser component excites the first fluorescence and the second fluorescence, and the processor is configured to adjust the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on the time difference, and obtain the detection result of the sample flow to be measured according to the adjusted parameters. For example, the processor may perform conversion of the delayed data of the first fluorescence receiver 310 and the second fluorescence receiver 320 according to the time difference and adjust the light intensity parameter according to the delayed data, or the processor may also adjust the light intensity parameter according to the time difference, the sequence of receiving the light intensity parameter by the first fluorescence receiver and the second fluorescence receiver 320, and the like, which is not limited herein.

The processor of the present embodiment is configured to perform signal processing and conversion on the fluorescence parameter or the light intensity parameter of the fluorescence detection component 30, so as to obtain a detection result of the sample flow to be detected. The processor may be referred to as a CPU (Central Processing Unit ), may be an electronic chip with signal processing capability, or may be a general purpose processor, a Digital Signal Processor (DSP), an Application Specific Integrated Circuit (ASIC), a Field Programmable Gate Array (FPGA) or other programmable logic device, discrete gate or transistor logic device, or discrete hardware components. General purpose processors include, but are not limited to, microprocessors or conventional processors.

In the embodiment of the present application, the processor of the optical measurement system adjusts the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on the time difference between the excitation of the first fluorescence and the second fluorescence by the laser component, so that signal crosstalk between the adjusted light intensity parameters does not occur, and the measurement accuracy of the optical measurement system is improved.

Optionally, the first fluorescence receiver 310 is configured to convert the light signal of the first fluorescence into a first light intensity parameter, and the second fluorescence receiver 320 is configured to convert the light signal of the second fluorescence into a second light intensity parameter.

The processor is further configured to receive the first light intensity parameter and the second light intensity parameter from the first fluorescence receiver 310 and the second fluorescence receiver 320 at a preset sampling frequency, delay at least one of the first light intensity parameter and the second light intensity parameter according to a sampling point, wherein the sampling point is obtained based on the sampling frequency and a time difference, and obtain a detection result based on the delayed first light intensity parameter and the delayed second light intensity parameter.

Specifically, the processor is connected to the first and second fluorescence receivers, respectively, and receives the first light intensity parameter from the first fluorescence receiver 310 and the second light intensity parameter from the second fluorescence receiver 320 at a preset sampling frequency. The processor may obtain the number of samples the processor samples the first fluorescence receiver 310 and the second fluorescence receiver based on the sampling frequency and the time difference, which is the amount of data obtained each time the processor samples, and in an alternative embodiment, the number of samples may be the product of the sampling frequency and the time difference. The processor delays at least one of the first light intensity parameter and the second light intensity parameter according to the sampling points, and obtains a detection result of the sample flow to be detected based on the delayed first light intensity parameter and the delayed second light intensity parameter.

In an alternative embodiment, when the processor delays at least one of the first light intensity parameter and the second light intensity parameter according to the number of sampling points, the processor may select one of the first light intensity parameter and the second light intensity parameter to delay according to a sequence of exciting the first fluorescence and the second fluorescence by the laser component. Illustratively, when the laser beam of the laser component irradiates the sample flow to be detected in the detection area and sequentially excites the first fluorescence and the second fluorescence, the first fluorescence receiver 310 receives the first light intensity parameter of the first fluorescence first, and the second fluorescence receiver 320 receives the second light intensity parameter of the second fluorescence first, and the processor is configured to delay the first light intensity parameter of the first fluorescence receiver 310 according to the number of sampling points, otherwise, the processor delays the second light intensity parameter of the second fluorescence receiver 320 according to the number of sampling points, which is not described herein again.

In the embodiment of the application, the processor is used for delaying at least one of the first light intensity parameter and the second light intensity parameter according to the number of sampling points, obtaining the detection result based on the delayed first light intensity parameter and the delayed second light intensity parameter, so that the first light intensity parameter and the second light intensity parameter in the detection result cannot be mutually crosstalked, and adjusting the distance between the first fluorescence and the second fluorescence in a delay mode of adjusting the first light intensity parameter and the second light intensity parameter can be used for more accurately adjusting the distance between the first fluorescence and the second fluorescence, thereby reducing the adjustment difficulty of the optical measurement system and further improving the detection accuracy of the optical measurement system.

Further, the laser component is used for emitting first laser and second laser with different wavelengths, the light beams of the first laser and the second laser irradiated on the detection area have a first height difference, and the processor is used for calculating the time difference based on the first height difference and the flow speed of the sample flow to be detected.

Specifically, the laser assembly may include a first laser and a second laser that are disposed at intervals and such that light beams of the emitted first laser and second laser do not overlap, such that light beam positions of the first laser and the second laser irradiated on the detection area have a first height difference, and the first laser and the second laser are time-divisionally irradiated on the detection area. The processor is used for calculating the time difference of the laser component for exciting the first fluorescence and the second fluorescence based on the first height difference and the flow velocity of the sample flow to be detected. It is understood that the sample stream to be measured flows in the detection area at a preset flow rate, and the time difference may be equal to the ratio of the first height difference and the flow rate of the sample stream to be measured in order to cause the same sample particle of the sample stream to be measured to flow from the irradiation area of the first laser light to the irradiation area of the second laser light and excite the first fluorescence and the second fluorescence.

In the embodiment of the application, the processor is used for calculating the time difference based on the first height difference and the flow velocity of the sample flow to be detected, so that the processor can delay at least one of the first light intensity parameter and the second light intensity parameter based on the time difference, thereby ensuring that the first light intensity parameter and the second light intensity parameter in the detection result cannot cross each other.

Further, the fluorescence detection assembly 30 further includes a light receiving member 350 disposed between the sheath flow assembly 10 and the fluorescence detection assembly 30, the light receiving member 350 is configured to amplify and collect the fluorescence beam excited by the sheath flow assembly 10, the first fluorescence forms a light spot with a first size on the light receiving surface of the first fluorescence receiver 310, and the second fluorescence forms a light spot with a second size on the light receiving surface of the second fluorescence receiver 320. The light receiving element 350 may be, but not limited to, a focusing lens, and the sample flow to be measured is irradiated by the laser component in the detection area to excite first fluorescence and second fluorescence, the first fluorescence is collected by the light receiving element 350 and irradiates the first fluorescence receiver 310 to form a light spot with a first size on the light receiving surface, and the second fluorescence is collected by the light receiving element 350 and irradiates the second fluorescence receiver 320 to form a light spot with a second size on the light receiving surface.

In the embodiment of the present application, the sum of the first dimension and the second dimension is less than or equal to 2 times of the product of the first height difference, the amplification factor of the light receiving element 350 and the flow rate of the sample flow to be measured, wherein the amplification factor of the light receiving element 350, the first dimension and the second dimension can be obtained from the assembled optical measurement system, the processor can calculate the first height difference based on the assembly mode of the optical measurement system, the flow rate of the sample flow to be measured, and the like, and the first height difference is greater than or equal to half of the sum of the first dimension and the second dimension divided by the amplification factor of the light receiving element 350 and the flow rate of the sample flow to be measured, so as to delay at least one of the first light intensity parameter and the second light intensity parameter based on the first height difference, thereby ensuring that the first light intensity parameter and the second light intensity parameter in the detection result do not cross-talk with each other.

In one embodiment, the first and second fluorescence receivers 310 and 320 are staggered and have a second height difference in the first direction that is greater than or equal to half of the sum of the spot sizes of the first and second fluorescence receivers 310 and 320.

Specifically, the present embodiment staggers the first fluorescence receiver 310 and the second fluorescence receiver 320 in the first direction when the first fluorescence receiver 310 and the second fluorescence receiver 320 are assembled, so that there is a second height difference between the first fluorescence receiver 310 and the second fluorescence receiver 320, the second height difference being greater than or equal to half of the sum value of the spot sizes of the first fluorescence receiver 310 and the second fluorescence receiver 320, so that the first fluorescence receiver 310 is not subject to crosstalk of the second light intensity parameter of the second fluorescence receiver 320 when receiving the first fluorescence, and the second fluorescence receiver 320 is not subject to crosstalk of the first light intensity parameter of the first fluorescence receiver 310 when receiving the second fluorescence.

Optionally, referring to fig. 2, fig. 2 is a schematic structural diagram of another embodiment of the optical measurement system provided by the present application. As shown in FIG. 2, the fluorescence detection assembly 30 further includes a third fluorescence receiver 330, and the laser assembly is configured to emit laser light with different wavelengths, so that the particle to be detected emits a third fluorescence. The first fluorescence receiver 310, the second fluorescence receiver 320, and the third fluorescence receiver 330 are staggered, the second fluorescence receiver 320 and the third fluorescence receiver 330 have a third height difference in the first direction, and the third height difference is greater than or equal to half of the sum of the spot sizes of the second fluorescence receiver 320 and the third fluorescence receiver 330.

Specifically, the laser assembly may further include a third laser disposed parallel to the first laser and the second laser, where the third laser is configured to emit third laser light and irradiate the third laser light to the detection area, so that the particles to be detected in the sample flow to be detected in the detection area excite third fluorescence, and wavelengths of the third laser light and the first laser light and the second laser light are different. It is understood that, since the third fluorescence is excited by the third laser light having the different wavelength, the third height difference, which the second fluorescence receiver 320 and the third fluorescence receiver 330 have in the first direction, may be determined based on the spot sizes of the second fluorescence receiver 320 and the third fluorescence receiver 330, and the third height difference is greater than or equal to half of the sum value of the spot sizes of the second fluorescence receiver 320 and the third fluorescence receiver 330.

It will be appreciated that in embodiments of the present application, the processor is configured to perform a quantitative analysis based on the light intensity parameters received by the first fluorescence receiver 310, and also configured to perform a classification analysis based on the light intensity parameters received by the second fluorescence receiver 320 and the third fluorescence receiver 330. The second laser and the third laser have a time difference when exciting the second fluorescence and the third fluorescence, and the time difference can be obtained by calculating according to a fourth height difference of the light beams irradiated on the detection area by the second laser and the third laser and the flow velocity of the sample flow to be detected, which are not described herein.

Specifically, each particle to be tested is coupled with at least one substance to be tested and magnetic beads, the magnetic beads are coated with a second fluorescent substance and a third fluorescent substance with intensity levels corresponding to the substances to be tested, and the classification analysis is to analyze the types of different substances to be tested based on the second fluorescent substance and the third classified fluorescent substance with different intensity levels. The number of the joint inspection corresponds to the number of the intensity grades of the second fluorescent substances and the third fluorescent substances coated by the magnetic beads, and the joint inspection of N-type second fluorescent substances and M-type third fluorescent substances can be realized. After the light intensity parameter is adjusted by the processor, quantitative information corresponding to the classification detection items in the sample to be detected at most N x M can be output.

In the embodiment of the present application, the first fluorescence receiver 310, the second fluorescence receiver 320, and the third fluorescence receiver 330 are staggered, and the second fluorescence receiver 320 and the third fluorescence receiver 330 have a third height difference in the first direction, so that the second fluorescence receiver 320 does not receive the crosstalk of the third fluorescence receiver 330 when receiving the second fluorescence, and the second fluorescence receiver 320 does not receive the crosstalk of the third fluorescence receiver 330 when receiving the second fluorescence.

In one embodiment, the optical measurement system further includes a processor, and the processor is connected to the fluorescence detection assembly 30, where the particle to be measured includes a magnetic bead and a first fluorescent substance, and the magnetic bead is coated with a second fluorescent substance with different intensity levels, where the first fluorescent substance is used for quantitative analysis, the second fluorescent substance is used for classification analysis, and the different intensity levels correspond to different classification detection items. The processor is configured to adjust the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on the time difference between the excitation of the first fluorescence and the second fluorescence by the laser component, so as to output quantitative information corresponding to each classification detection item of the sample to be detected.

Specifically, in the optical measurement system of this embodiment, the second fluorescence excited by the second fluorescent material is collected by the second fluorescent receiver 320 and the second light intensity data is obtained, the first fluorescent receiver 310 also collects the first fluorescence excited by the first fluorescent material and obtains the first light intensity data, and the processor adjusts the first light intensity data and the second light intensity data based on the time difference between the excitation of the first fluorescent material and the excitation of the second fluorescent material by the laser component, so that the adjusted first light intensity data and the adjusted second light intensity data do not cross-talk with each other. The processor classifies each detection item based on the second light intensity data under different intensity levels, and outputs corresponding quantitative information based on the first light intensity data corresponding to the classified detection item, so as to realize classification analysis and quantitative analysis of the sample flow to be detected.

Each particle to be measured is coupled with at least one substance to be measured and magnetic beads, and the magnetic beads are coated with a second fluorescent substance with intensity level corresponding to the substance to be measured, wherein the quantitative analysis can be used for analyzing the concentration of the substance to be measured in the sample flow to be measured. The substances to be measured can be various antigens or antibodies in the blood sample, such as antigen A/B/C/D/and the like, or other substances needing to be classified and counted, and each substance to be measured corresponds to one classified detection item. The classification analysis may be an analysis of the class of different substances to be measured in the sample stream to be measured based on the second fluorescent substances of different intensity levels. The first fluorescence receiver 310 and the second fluorescence receiver 320 are staggered along the first direction of the sheath flow assembly 10 and have a second height difference, and it is understood that in order to enhance the effect of fluorescence collection by the fluorescence receiver, when the wavelength band of the first fluorescence is smaller than the second fluorescence, the sheath flow assembly 10, the first fluorescence receiver 310, and the second fluorescence receiver 320 may be sequentially arranged along the first direction, i.e., the distance between the first fluorescence receiver 310 and the sheath flow assembly 10 is shorter, and vice versa.

It can be understood that, by the optical measurement system provided by the above embodiment, joint inspection of a plurality of immune projects can be realized, and the number of joint inspection corresponds to the number of intensity levels of the second fluorescent substances coated by the magnetic beads, that is, the joint inspection of N substances to be detected can be realized by the second fluorescent substances with N intensity levels.

In the embodiment of the application, the processor adjusts the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on the time difference between the excitation of the first fluorescence and the second fluorescence by the laser component, so as to output quantitative information corresponding to each classified detection item of the sample to be detected, so that crosstalk can not occur between the classified analysis and the quantitative analysis of the optical measurement system, and the accuracy of the quantitative information is improved.

Optionally, the magnetic beads are coated with a second fluorescent material and a third fluorescent material with different intensity levels, and the fluorescence detection assembly 30 further includes a third fluorescent material receiver 330, where the second fluorescent material and the third fluorescent material are irradiated by the same laser light of the laser assembly, so that the second fluorescent material receiver 320 receives the fluorescence excited by the second fluorescent material, and the third fluorescent material receiver 330 receives the fluorescence excited by the third fluorescent material. The second fluorescence receiver 320 and the third fluorescence receiver 330 are juxtaposed in a direction perpendicular to the first direction.

Specifically, in the present embodiment, the laser assembly includes a first laser and a second laser, the first laser light emitted by the first laser light of the first fluorescent substance of the sample flow to be measured excites the first fluorescent light, the second laser light emitted by the second laser light of the second fluorescent substance of the sample flow to be measured excites the second fluorescent light, and the second laser light emitted by the second laser light of the third fluorescent substance of the sample flow to be measured excites the third fluorescent light, the first fluorescent light receiver 310 is used for receiving the first fluorescent light, the second fluorescent light receiver 320 is used for receiving the second fluorescent light, and the third fluorescent light receiver 330 is used for receiving the third fluorescent light.

In this embodiment, each particle to be measured is coupled with at least one substance to be measured and a magnetic bead, the magnetic bead is coated with a second fluorescent substance and a third fluorescent substance with intensity levels corresponding to the substance to be measured, and the classification analysis is to analyze the types of different substances to be measured based on the second fluorescent substance and the third classified fluorescent substance with different intensity levels. The number of the joint inspection corresponds to the number of the intensity grades of the second fluorescent substances and the third fluorescent substances coated by the magnetic beads, and the joint inspection of N-type second fluorescent substances and M-type third fluorescent substances can be realized. After the light intensity parameter is adjusted by the processor, quantitative information corresponding to the classification detection items in the sample to be detected at most N x M can be output.

It can be understood that in this embodiment, the second fluorescent material and the third fluorescent material coated by the magnetic beads are excited by the same laser (the same wavelength), so that multiple lasers are not required to be arranged to excite the classified fluorescence, and joint inspection of multiple classified items can be realized at a greatly reduced cost of the optical measurement system. Since the fluorescence received by the second fluorescence receiver 320 and the third fluorescence receiver 330 is excited by the laser irradiation of the same laser (the same wavelength), the second fluorescence receiver 320 and the third fluorescence receiver 330 may be disposed at the same height in the first direction, such that the second fluorescence receiver 320 and the third fluorescence receiver 330 are disposed in parallel in the vertical direction of the first direction, because the second fluorescence receiver 320 and the third fluorescence receiver 330 have no time difference when generated in the detection region. Because the fluorescence received by the first fluorescence receiver 310 and the fluorescence received by the second fluorescence receiver 320 and the third fluorescence receiver 330 are obtained by the excitation of the lasers of different lasers, the second height difference is arranged between the first fluorescence receiver 310 and the second fluorescence receiver 320 and between the first fluorescence receiver 310 and the third fluorescence receiver 330, so that the crosstalk between different light beams can be reduced, and the detection accuracy is improved on the basis of realizing low-cost multi-joint detection.

In an embodiment, the fluorescence detection assembly 30 further includes a light receiving member 350 and at least one light splitting member 340, the light receiving member 350 is disposed on one side of the sheath flow assembly 10, the light splitting member 340 is disposed on one side of the light receiving member 350 away from the sheath flow assembly 10, the first fluorescence receiver 310 is disposed on one side of the light splitting member 340 and is perpendicular to the light receiving member 350, the second fluorescence receiver 320 is disposed on one side of the light splitting member 340 away from the light receiving member 350, the light receiving member 350 is used for amplifying and collecting the fluorescence beam excited by the sheath flow assembly 10, and the light splitting member 340 is used for splitting the fluorescence beam into first fluorescence and second fluorescence with different wavelengths.

Specifically, the beam splitter 340 includes, but is not limited to, a dichroic mirror, and the beam splitter 340 is configured to transmit the fluorescent light beam of the first wavelength band and reflect the fluorescent light beam of the second wavelength band. For example, when the fluorescence detecting assembly 30 includes the first fluorescence receiver 310 and the second fluorescence receiver 320, and the fluorescence detecting assembly 30 includes one light splitting device 340, the sheath flow assembly 10, the light receiving device 350, and the light splitting device 340 are sequentially disposed along the first direction, and the fluorescence beam emitted from the sheath flow assembly 10 is collected and emitted to the light splitting device 340 through the light receiving device 350, so that the fluorescence beam is split into the first fluorescence and the second fluorescence, the first fluorescence is reflected by the light splitting device 340 and emitted into the first fluorescence receiver 310 along the vertical direction of the first direction, and the second fluorescence is transmitted from the light splitting device 340 and emitted into the second fluorescence receiver 320 along the first direction.

Referring to fig. 3, fig. 3 is a schematic structural diagram of an optical measurement system according to another embodiment of the present application. As shown in fig. 3, in an alternative embodiment, the fluorescence detection assembly 30 may include n fluorescence receivers and n-1 light splitting members 340, and the sheath flow assembly 10, the light receiving member 350, the first light splitting member 340, the second light splitting member 340, the first light splitting member 340, and the nth light splitting member 340 are sequentially disposed in the first direction. For example, when the fluorescence detecting assembly 30 includes two light splitting devices 340, a first fluorescence receiver 310, a second fluorescence receiver 320, and a third fluorescence receiver 330, the sheath flow assembly 10, the light receiving device 350, the first light splitting device 340, and the second light splitting device 340 are sequentially disposed along the first direction, and the second light splitting device 340 is configured to receive the fluorescence beam transmitted by the first light splitting device 340 and split the fluorescence beam into the second fluorescence and the third fluorescence so that the second fluorescence receiver 320 receives the second fluorescence and the third fluorescence receiver 330 receives the third fluorescence.

Optionally, in the optical path direction of the fluorescent light beam, a diaphragm is further disposed at the front side of the fluorescent receiver, and the diaphragm is used for filtering stray light inside the optical measurement system, so as to reduce interference of the stray light. For example, when the fluorescence detection assembly 30 includes one light-splitting member 340, the optical measurement system further includes a first diaphragm 311 and a second diaphragm 321, the first diaphragm 311 is disposed between the light-splitting member 340 and the first fluorescence receiver 310, and the second diaphragm 321 is disposed between the light-splitting member 340 and the second fluorescence receiver 320. The first diaphragm 311 is configured to receive the first fluorescent light reflected by the light splitting element 340 and filter stray light of the first fluorescent light, and the first fluorescent light located in a preset band is projected onto a light receiving surface of the first fluorescent receiver 310 through the first diaphragm 311 to be received by the first fluorescent receiver 310. The second diaphragm 321 is configured to receive the second fluorescent light transmitted by the light splitting component 340 and filter stray light of the second fluorescent light, and the second fluorescent light located in the preset band is projected onto the light receiving surface of the second fluorescent receiver 320 through the second diaphragm 321. Similarly, when the fluorescence detection assembly 30 further includes the third fluorescence receiver 330 and the third diaphragm 331, the third diaphragm 331 is configured to receive the third fluorescence transmitted by the light splitting component 340 and filter stray light of the third fluorescence, which is not described herein.

Further, a filter is also arranged between the diaphragm and the fluorescence receiver. Specifically, when the fluorescence detection assembly 30 includes one light splitting device 340, the fluorescence detection assembly 30 further includes a first optical filter 312 and a second optical filter 322, the first optical filter 312 corresponds to the first fluorescence receiver 310, and the second optical filter 322 corresponds to the second fluorescence receiver 320. The first filter 312 filters light beams of the first wavelength band and cuts off light beams outside the first wavelength band, and the second filter 322 filters light beams of the second wavelength band and cuts off light beams outside the second wavelength band. The wavelength ranges of the first filter 312 and the second filter 322 do not cross, and the cut-off wavelength range OD is greater than 6, so that fluorescence between the first fluorescence receiver 310 and the second fluorescence receiver 320 does not cross, and mutual crosstalk is not easily generated. Similarly, when the fluorescence detection assembly 30 further includes the third fluorescence receiver 330 and the third optical filter 332, the third optical filter 332 is configured to enable the light beam of the specific wavelength band to penetrate through and be disposed on the light receiving surface of the third fluorescence receiver 330, which is not described herein again.

The application also provides a sample analyzer, which comprises a sample injection module, a sampling module and the optical measurement system of any embodiment. The optical measurement system is used for carrying out classification analysis and quantitative analysis on the sample to be detected so as to obtain a detection result of the sample to be detected.

Specifically, the sample injection module may be used to obtain a sample rack, on which a batch of sample tubes are placed, and move the sample tubes to be detected to sampling positions corresponding to the sample modules. The sampling module can absorb the sample to be tested in the sample tube through the sampling needle. The sample analyzer may also be provided with a sample preparation module for mixing a sample to be tested with a detection reagent and preparing a sample stream to be tested. The particles to be measured in the sample flow to be measured are arranged one by one and pass through the detection area of the sheath flow assembly 10, so that the laser assembly irradiates the particles to be measured passing one by one and obtains second fluorescence for performing classification analysis and first fluorescence for performing quantitative analysis. The processor is used for adjusting light intensity data corresponding to at least one of the first fluorescence and the second fluorescence based on the time difference of the excitation component exciting the first fluorescence and the second fluorescence, and obtaining a detection result.

In the embodiment of the application, the first fluorescence receiver 310 and the second fluorescence receiver 320 for collecting fluorescence are arranged at intervals in the first direction, so that the crosstalk degree between the first fluorescence and the second fluorescence is reduced, the fluorescence collection performance of the first fluorescence receiver 310 and the second fluorescence receiver 320 is improved, and the measurement accuracy of the optical measurement system is further improved.

The foregoing description is only of embodiments of the present application, and is not intended to limit the scope of the application, and all equivalent structures or equivalent processes using the descriptions and the drawings of the present application or directly or indirectly applied to other related technical fields are included in the scope of the present application.

Claims (10)

1. An optical measurement system, comprising:

a sheath flow assembly comprising a detection zone for passing a sample stream to be tested in a first direction, the sample stream to be tested comprising a plurality of particles to be tested;

the laser component is arranged at one side of the sheath flow component and is used for emitting laser to the detection area so as to enable particles to be detected in the detection area to emit fluorescence;

The fluorescence detection component is arranged at one side of the sheath flow component far away from the laser component and is used for receiving fluorescence excited by the particles to be detected irradiated by the laser;

The laser component is used for emitting lasers with different wavelengths so that the particles to be detected respectively excite first fluorescence and second fluorescence, the fluorescence detection component comprises a first fluorescence receiver used for receiving the first fluorescence and a second fluorescence receiver used for receiving the second fluorescence, and the first fluorescence receiver and the second fluorescence receiver are arranged at intervals along the first direction.

2. The optical measurement system of claim 1, further comprising a processor coupled to the fluorescence detection assembly;

The processor is used for adjusting the light intensity parameters received by the first fluorescence receiver and the second fluorescence receiver based on the time difference between the excitation of the first fluorescence and the second fluorescence of the laser component so as to obtain the detection result of the sample flow to be detected.

3. The optical measurement system of claim 2, wherein the first fluorescence receiver is configured to convert the optical signal of the first fluorescence light into a first light intensity parameter, the second fluorescence receiver is configured to convert the optical signal of the second fluorescence light into a second light intensity parameter, and the processor is further configured to:

receiving the first and second light intensity parameters from the first and second fluorescence receivers at a preset sampling frequency;

Delaying at least one of the first light intensity parameter and the second light intensity parameter according to a sampling point number, wherein the sampling point number is obtained based on the sampling frequency and the time difference;

And obtaining the detection result based on the first light intensity parameter and the second light intensity parameter after the delay processing.

4. The optical measurement system of claim 3 wherein the laser assembly is configured to emit first and second lasers of different wavelengths, the first and second laser beams impinging on the detection zone having a first height differential, the processor being configured to calculate the time differential based on the first height differential and the flow rate of the sample stream to be measured.

5. The optical measurement system of claim 4, wherein the fluorescence detection assembly further comprises a light receiving member disposed between the sheath flow assembly and the fluorescence detection assembly, the light receiving member configured to amplify and collect a fluorescent light beam excited by the sheath flow assembly, the first fluorescent light forming a first size spot on the light receiving surface of the first fluorescence receiver, the second fluorescent light forming a second size spot on the light receiving surface of the second fluorescence receiver;

And the sum of the first size and the second size is smaller than or equal to 2 times of the product of the first height difference, the magnification of the light receiving piece and the flow speed of the sample flow to be detected.

6. The optical measurement system of claim 1, wherein the first and second fluorescence receivers are staggered and have a second height difference in the first direction that is greater than or equal to half of a sum of spot sizes of the first and second fluorescence receivers.

7. The optical measurement system of claim 6, wherein the fluorescence detection assembly further comprises a third fluorescence receiver, the laser assembly configured to emit laser light having a different wavelength to excite the particle under test to emit a third fluorescence;

The first fluorescence receiver, the second fluorescence receiver and the third fluorescence receiver are arranged in a staggered mode, the second fluorescence receiver and the third fluorescence receiver are provided with a third height difference in the first direction, and the third height difference is larger than or equal to half of the sum value of the spot sizes of the second fluorescence receiver and the third fluorescence receiver.

8. The optical measurement system of any one of claims 1 to 6, further comprising a processor coupled to the fluorescence detection assembly;

The particles to be detected comprise magnetic beads and first fluorescent substances, the magnetic beads are coated with second fluorescent substances with different intensity levels, the first fluorescent substances are used for quantitative analysis, the second fluorescent substances are used for classification analysis, and different intensity levels correspond to different classification detection items;

The processor is used for adjusting the light intensity parameters received by the first fluorescence receiver and the second fluorescence receiver based on the time difference of the first fluorescence excited by the laser component and the second fluorescence excited by the laser component so as to output quantitative information corresponding to each classified detection item of the sample to be detected.

9. The optical measurement system of claim 8, wherein the magnetic beads are coated with a second fluorescent material and a third fluorescent material having different intensity levels, the fluorescence detection assembly further comprising a third fluorescent material, the second fluorescent material and the third fluorescent material being irradiated by the same laser light of the laser assembly such that the second fluorescent material receives fluorescence excited by the second fluorescent material and the third fluorescent material receives fluorescence excited by the third fluorescent material;

The second fluorescent receiver and the third fluorescent receiver are arranged in parallel in the vertical direction of the first direction.

10. A sample analyzer, comprising:

The sample injection module is used for acquiring a sample tube to be detected;

The sampling module is used for sampling the sample tube so as to acquire and convey a sample to be detected of the sample tube;

The optical measurement system according to any one of claims 1 to 9, for performing a classification analysis and a quantitative analysis on the sample to be measured to obtain a detection result of the sample to be measured.