CN1298741C - Process for extracting protein, total flavone and super oxide dismutase from sea-buckthorn fresh branch and leaf - Google Patents
Process for extracting protein, total flavone and super oxide dismutase from sea-buckthorn fresh branch and leaf Download PDFInfo
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- CN1298741C CN1298741C CNB2005100113655A CN200510011365A CN1298741C CN 1298741 C CN1298741 C CN 1298741C CN B2005100113655 A CNB2005100113655 A CN B2005100113655A CN 200510011365 A CN200510011365 A CN 200510011365A CN 1298741 C CN1298741 C CN 1298741C
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- sea
- leaf
- buckthorn
- protein
- total flavones
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- 229930003944 flavone Natural products 0.000 title claims abstract description 44
- 235000011949 flavones Nutrition 0.000 title claims abstract description 44
- 235000003145 Hippophae rhamnoides Nutrition 0.000 title claims abstract description 39
- 102000019197 Superoxide Dismutase Human genes 0.000 title claims abstract description 23
- 108010012715 Superoxide dismutase Proteins 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 20
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 19
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 19
- 229940032362 superoxide dismutase Drugs 0.000 title claims description 11
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title abstract 6
- 150000002212 flavone derivatives Chemical class 0.000 title abstract 6
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title abstract 6
- 240000000950 Hippophae rhamnoides Species 0.000 title 1
- 241000229143 Hippophae Species 0.000 claims abstract description 41
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 26
- 239000012043 crude product Substances 0.000 claims abstract description 19
- 101710138460 Leaf protein Proteins 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 8
- 238000005516 engineering process Methods 0.000 claims abstract description 8
- 239000012065 filter cake Substances 0.000 claims abstract description 8
- 238000000605 extraction Methods 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 150000002213 flavones Chemical class 0.000 claims description 38
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 229940088598 enzyme Drugs 0.000 claims description 9
- 239000000284 extract Substances 0.000 claims description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 6
- 230000035613 defoliation Effects 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 238000009833 condensation Methods 0.000 claims description 3
- 230000005494 condensation Effects 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000008176 lyophilized powder Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 230000008901 benefit Effects 0.000 abstract description 7
- 235000019750 Crude protein Nutrition 0.000 abstract 1
- 238000005189 flocculation Methods 0.000 abstract 1
- 230000016615 flocculation Effects 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000004898 kneading Methods 0.000 abstract 1
- 238000010298 pulverizing process Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 235000003935 Hippophae Nutrition 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
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- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Seasonings (AREA)
Abstract
The present invention relates to a process for extracting protein, total flavone and superoxide dismutase from fresh tender sea-buckthorn branches and leaves, which mainly comprises steps: selecting, striking, cutting, kneading, squeezing and filtering the tender sea-buckthorn branches and leaves with a 30-mesh sieve to obtain branch and leaf juice; heating the branch and leaf juice to the solidifying point of the protein, maintaining for 30 minutes, filtering to collect leaf protein flocculation, pressing by filtration to obtain filter cake, and drying to obtain crude leaf protein powder; combining the hot leaf juice and the filter-pressed hot leaf juice together, extracting a crude product of the total flavone, drying and pulverizing to obtain crude total flavone powder; extracting SOD from the concentrated leaf juice with the total flavone eliminated to obtain crude freeze-dried sea-buckthorn tender branch and leaf Cu, Zn -SOD powder. The obtained crude protein powder, the crude total flavone and the crude SOD can be purified according to different purity requirements through corresponding technologies. In the extraction process of the present invention, the low-priced tender sea-buckthorn branches and leaves are used as raw materials, the cost is low, and all the three extracted substances have great practical economical benefits and social benefits.
Description
Technical field
The invention belongs to the technical field of extracting Fructus Hippophae total flavones from Folium Hippophae, specially refer to a kind of method of extracting protein, total flavones and superoxide-dismutase from the fresh and tender branches and leaves of sea-buckthorn.
Background technology
For a long time, to the not fully comprehensive utilization of sea-buckthorn branches and leaves, in production test, only be confined to extract total flavones.At present, still beyond example for the technology of extracting protein, total flavones and superoxide-dismutase (SOD) from the fresh and tender branches and leaves of sea-buckthorn.The fresh and tender branches and leaves rich in proteins of sea-buckthorn (the dry-matter percentage composition is greater than 17%), total flavones (the dry-matter percentage composition is greater than 1%) and SOD enzyme.In the extraction industry of other plant leaf protein matter, be not fully used for the juice behind the extraction protein yet, both wasted resource, cause environmental pollution again.Therefore, for developing a kind of technology of extracting protein, total flavones and superoxide-dismutase from the fresh and tender branches and leaves of sea-buckthorn, to guarantee to extract Fructus Hippophae total flavones, the unlikely destruction of SOD enzyme in the clear juice of leaf behind the leaf protein, by suitable technological process, extract protein, total flavones, SOD enzyme respectively, to improve the benefit of sea-buckthorn browse processing, three kinds of materials of this that is extracted all have bigger practical value.
Summary of the invention
Purpose of the present invention: be to provide a kind of method of extracting protein, total flavones and superoxide-dismutase from the fresh and tender branches and leaves of sea-buckthorn, sea-buckthorn browse with cheapness is a raw material, by simple technology, safety in production, production method easy to implement, the above-mentioned three kinds of materials that extracted all have bigger practical value.
To achieve these goals, the present invention realizes by the following technical solutions: a kind of method from the fresh and tender branches and leaves extraction of sea-buckthorn protein, total flavones and superoxide-dismutase is a main raw material with the sea-buckthorn browse, it is characterized in that:
(1), sea-buckthorn browse process is selected, hammered into shape and cut, rub, squeeze, with 30 mesh filter screens filtration debranching leaf slag, to obtain sea-buckthorn browse juice;
(2), above sea-buckthorn browse juice is arrived under 60～93 ℃ of temperature of proteic hot condensation point with heat exchanger heats, and in container, keep after 30 minutes, filter the collection leaf protein with 60 mesh filter screens and coagulate wadding, leaf protein coagulates wadding and obtain filter cake behind 120 order filter clothes, 0.1Mpa filtering under pressure, with filter cake bake drying under 120～130 ℃ of temperature, crushed after being dried, to obtain leaf protein powder crude product;
(3), with the above clear juice merging of hot leaf through removing the clear juice of hot leaf after leaf protein coagulates wadding and going out through press filtration, extract the technological process of total flavones again, in the easy molten hot water of the total flavones in the sea-buckthorn browse, the SOD enzyme can tolerate 95 ℃ of temperature, still survive in the clear juice of leaf behind the defoliation albumen, leaf behind the defoliation albumen clear juice process-600mmHg negative pressure and 60 ℃ of temperature, concentrating under reduced pressure is to 1/3 postcooling to 4 of original volume ℃, and kept 2～3 hours, with the horizontal spiral separating centrifuge of separating factor greater than 3500G, centrifugation total flavones precipitation, isolated total flavones crude product is being pulverized behind the bake drying under 85 ℃ of temperature, to obtain sea-buckthorn browse total flavones powder crude product;
(4), with above concentrated Ye Qingzhi after removing total flavones, extract the technological process of SOD enzyme again, the concentrated Ye Qingzhi that removes behind the total flavones is added ammonium sulfate (NH down at 4 ℃
4)
2SO
4Saturation concentration to 85%, and after keeping 4 hours, the SOD enzyme of being separated out greater than the tubular type ultra-high speed separating centrifuge reclaim(ed) sulfuric acid ammonium salt of 6000G with separating factor precipitates, the Cu that reclaims, the Zn-SOD enzyme is deposited under-17 ℃ of temperature ,-the 600mmHg negative pressure under lyophilization, to obtain sea-buckthorn browse Cu, Zn-SOD enzyme crude product lyophilized powder.
The above raw protein powder that obtains, crude product total flavones, crude product SOD enzyme according to the requirement of different purity, carry out corresponding technology purification again and get final product.
Advantage of the present invention and positively effect:
The present invention is to be main raw material with the fresh and tender branches and leaves of sea-buckthorn, extracts the method for protein, total flavones and superoxide-dismutase, and three kinds of materials that extracted all have bigger practical value.Improve the comprehensive utilization processing benefit of sea-buckthorn browse, not only complete processing is simple, and production operation safety, environmental pollution reduces, the present invention is that the sea-buckthorn browse with cheapness is raw material and extraction process cheaply, and the above-mentioned three kinds of materials that extracted all have bigger practical economic benefit and social benefit.
Description of drawings
Fig. 1 provides process flow sheet of the present invention.
Embodiment
Below provide embodiment by Fig. 1:
(1) sea-buckthorn browse process is selected, hammered into shape and cut, rub, squeeze, filter debranching leaf slag with 30 mesh filter screens, to obtain sea-buckthorn browse juice;
(2) above sea-buckthorn browse juice is arrived under 60～93 ℃ of temperature of proteic hot condensation point with heat exchanger heats, kept 30 minutes, filter the collection leaf protein with 60 mesh filter screens and coagulate wadding, leaf protein coagulates wadding and obtain filter cake after 120 order filter clothes, 0.1Mpa press filtration, with filter cake bake drying under 120～130 ℃ of temperature, crushed after being dried, reach leaf protein powder crude product more than 65% to obtain purity of protein, yield reach bright leaf heavy about 3%;
(3) above clear juice of heat and filter cake after removing leaf protein to coagulate wadding merged through the clear juice of heat that press filtration goes out, extract the technological process of total flavones again, in the easy molten hot water of the total flavones in the sea-buckthorn browse, the SOD enzyme can tolerate 95 ℃ of temperature, still survive in the clear juice behind the defoliation albumen, clear juice process-600mmHg negative pressure behind the defoliation albumen and 60 ℃ of following concentrating under reduced pressure to original volume 1/3 after, be cooled to 4 ℃, and kept 2～3 hours, with the horizontal spiral separating centrifuge of separating factor greater than 3500G, centrifugation goes out the total flavones precipitation, isolated total flavones crude product is being pulverized behind the bake drying under 85 ℃ of temperature, obtaining the total flavones powder crude product of sea-buckthorn browse flavones content more than 20%, yield close bright leaf heavy about 1.2%;
(4) with above concentrated clear juice after removing total flavones, extract the technological process of SOD enzyme again, the concentrated clear juice behind the removal total flavones under 4 ℃, is added ammonium sulfate (NH
4)
2SO
4Saturation concentration to 85%, and after keeping 4 hours, the SOD enzyme of being separated out greater than the tubular type ultra-high speed separating centrifuge reclaim(ed) sulfuric acid ammonium salt of 6000G with separating factor precipitates, the Cu that reclaims, the Zn-SOD enzyme is deposited under-17 ℃ of temperature ,-the 600mmHg negative pressure under lyophilization, obtaining the sea-buckthorn browse than the above Cu of 1600Ul/mg alive, Zn-SOD enzyme crude product lyophilized powder, yield close bright leaf heavy about 3 ‰.
The above raw protein powder that obtains, crude product total flavones, crude product SOD enzyme according to the requirement of different purity, carry out corresponding technology purification again and get final product.
More than used equipment and material be commercial prod.
Claims (2)
1, a kind of method from the fresh and tender branches and leaves extraction of sea-buckthorn protein, total flavones and superoxide-dismutase is a main raw material with the sea-buckthorn browse, it is characterized in that:
(1), sea-buckthorn browse process is selected, hammered into shape and cut, rub, squeeze, with 30 mesh filter screens filtration debranching leaf slag, to obtain sea-buckthorn browse juice;
(2), above sea-buckthorn browse juice is arrived under 60～93 ℃ of temperature of proteic hot condensation point with heat exchanger heats, and in container, keep after 30 minutes, filter the collection leaf protein with 60 mesh filter screens and coagulate wadding, leaf protein coagulates wadding and obtain filter cake behind 120 order filter clothes, 0.1Mpa filtering under pressure, with filter cake bake drying under 120～130 ℃ of temperature, crushed after being dried, to obtain leaf protein powder crude product;
(3), with the above clear juice merging of hot leaf through removing the clear juice of hot leaf after leaf protein coagulates wadding and going out through press filtration, extract the technological process of total flavones again, leaf behind the defoliation albumen clear juice process-600mmHg negative pressure and 60 ℃ of temperature, concentrating under reduced pressure is to 1/3 postcooling to 4 of original volume ℃, and kept 2～3 hours, with the horizontal spiral separating centrifuge of separating factor greater than 3500G, centrifugation total flavones precipitation, isolated total flavones crude product is being pulverized behind the bake drying under 85 ℃ of temperature, to obtain sea-buckthorn browse total flavones powder crude product;
(4), with above concentrated Ye Qingzhi after removing total flavones, extract the technological process of SOD enzyme again, the concentrated Ye Qingzhi that removes behind the total flavones is added ammonium sulfate (NH down at 4 ℃
4)
2SO
4Saturation concentration to 85%, and after keeping 4 hours, the SOD enzyme of being separated out greater than the tubular type ultra-high speed separating centrifuge reclaim(ed) sulfuric acid ammonium salt of 6000G with separating factor precipitates, the Cu that reclaims, the Zn-SOD enzyme is deposited under-17 ℃ of temperature ,-the 600mmHg negative pressure under lyophilization, to obtain sea-buckthorn browse Cu, Zn-SOD enzyme crude product lyophilized powder.
2, the method for extracting protein, total flavones and superoxide-dismutase from the fresh and tender branches and leaves of sea-buckthorn according to claim 1, it is characterized in that: the raw protein powder that is obtained, crude product total flavones, crude product SOD enzyme, according to the requirement of different purity, carry out corresponding technology again and purify.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100113655A CN1298741C (en) | 2005-02-28 | 2005-02-28 | Process for extracting protein, total flavone and super oxide dismutase from sea-buckthorn fresh branch and leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100113655A CN1298741C (en) | 2005-02-28 | 2005-02-28 | Process for extracting protein, total flavone and super oxide dismutase from sea-buckthorn fresh branch and leaf |
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| Publication Number | Publication Date |
|---|---|
| CN1683397A CN1683397A (en) | 2005-10-19 |
| CN1298741C true CN1298741C (en) | 2007-02-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2005100113655A Expired - Fee Related CN1298741C (en) | 2005-02-28 | 2005-02-28 | Process for extracting protein, total flavone and super oxide dismutase from sea-buckthorn fresh branch and leaf |
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| Country | Link |
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| CN (1) | CN1298741C (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1943391B (en) * | 2006-11-09 | 2010-05-12 | 武汉光盛生物工程技术开发有限公司 | A kind of compound extraction method of plant leaf protein |
| CN101084943B (en) * | 2007-06-13 | 2011-03-23 | 青海清华博众生物技术有限公司 | Method for preparing mixture containing natural vitamin P from sea-buckthorn |
| US9706787B2 (en) | 2007-10-05 | 2017-07-18 | Advance International Inc. | Systems and methods for deriving protein powder |
| US8663725B2 (en) | 2007-10-05 | 2014-03-04 | Advance International Inc. | Method for deriving a high-protein powder/ omega 3 oil and double distilled water from any kind of fish or animal ( protein) |
| US9826757B2 (en) | 2013-03-15 | 2017-11-28 | Advance International Inc. | Automated method and system for recovering protein powder meal, pure omega 3 oil and purified distilled water from animal tissue |
| CA2920789C (en) | 2015-06-04 | 2021-05-18 | Advance International, Inc. | Improved methods and systems for recovering protein powder and natural omega-3 oil from animal tissue |
| CN107595902A (en) * | 2017-09-28 | 2018-01-19 | 成都中医药大学 | A kind of extracting method of sea-buckthorn branches and leaves general flavone |
| CN116076433A (en) * | 2018-07-04 | 2023-05-09 | 长沙谷奥生物科技有限公司 | Application of SOD and SOD offal and method for raising poultry by using superoxide dismutase to replace antibiotics |
| CN110585261A (en) * | 2019-09-25 | 2019-12-20 | 新疆超越歆生物科技有限公司 | Process preparation method of sea-buckthorn blueberry instant soft capsule |
| CN110643582B (en) * | 2019-09-29 | 2021-07-27 | 浙江工业大学 | A kind of method for extracting SOD from fresh sea buckthorn fruit |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1083863A (en) * | 1992-09-09 | 1994-03-16 | 中国科学院沈阳应用生态研究所 | By the sea-buckthorn technology for extrating and separating superoxide dismutase |
| CN1546483A (en) * | 2003-12-11 | 2004-11-17 | 水利部沙棘开发管理中心 | Method for extracting high purity seabuckthorn flavone aglycone |
-
2005
- 2005-02-28 CN CNB2005100113655A patent/CN1298741C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1083863A (en) * | 1992-09-09 | 1994-03-16 | 中国科学院沈阳应用生态研究所 | By the sea-buckthorn technology for extrating and separating superoxide dismutase |
| CN1546483A (en) * | 2003-12-11 | 2004-11-17 | 水利部沙棘开发管理中心 | Method for extracting high purity seabuckthorn flavone aglycone |
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| CN1683397A (en) | 2005-10-19 |
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