CN1289092C - Intestine micro ecological regulating agent - Google Patents

Abstract

The present invention provides a microecological regulating agent for intestinal tracts, which belongs to the field of microecological preparation. The regulating agent is mainly prepared from bacillus bifidus powder, lactobacillus acidophilus powder and stachyose. The regulating agent is characterized in that the regulating agent comprises the components of the following parts by weight: 10% to 90% of stachyose (containing raffinose), 5% to 75% of bacillus bifidus powder and lactobacillus acidophilus powder and 0 to 30% of additive. The regulating agent can achieve the purposes of regulating the immunity of a body, delaying senility, suppressing tumor, regulating blood fat and improving the stomach and the intestine through directly replenishing bacillus bifidus and lactobacillus acidophilus for the intestinal tracts of a human body. Simultaneously, a specific promotion substance-stachyose is provided so that the two beneficial bacteria after entering the intestinal tracts can be quickly activated and proliferated to adjust and improve the microecological system of the intestinal tracts.

Description

A kind of intestinal microecology regulator

Technical field the present invention relates to a kind of intestinal microecology regulator, belongs to the category of microbial ecological agent.Microecologic regulator is to regulate the equilibrated preparation of microecosystem in the host, and it can play the effect that improves the host health level.Microbial ecological agent can be divided into probiotics (Probiotic), benefit is given birth to three kinds of matter (Prebiotic), Synbiotics (Synbiotic) by constituting, the present invention is a kind of Synbiotics, bacillus bifidus, bacillus acidophilus and stachyose are organically combined, and can breed rapidly after making these two kinds of probioticss enter intestinal, quick adjustment and improve the intestinal microecology system reaches and regulates immunity, suppresses tumor, blood lipid regulation, improves a series of treatments and health-care effect such as function of intestinal canal.

Background technology since 20th century mankind to little ecological Studies so far, microecology has obtained significant progress, especially the application of aspects such as its prevention in disease, treatment, health care has obtained paying attention to widely, studies show that in a large number: the digestion of the nutrition of normal flora and human body and be absorbed with substantial connection in the human body intestinal canal, normal flora has been participated in the absorption and the metabolism of cholesterol, steroid, fat, protein, lipoid, aminoacid and some drugs.The metabolite of bifidobacterium fermentation sugar produces a large amount of acetic acid and lactic acid, the low pH and suboxides reduction potential (Eh) environment that cause intestinal, this helps the absorption of ferrum, calcium and vitamin D very much, the sour environment that the while fermenting carbohydrate causes can suppress many pathogen, can also promote enterokinesia, prevent the transition breeding of antibacterial.The multiple antigenic substance that normal flora produced can stimulate body generation immunne response, makes immune system keep state of activation, plays immunization.Research is also found: bacillus bifidus can degrade nitrous acid ammonia and nitrite, eliminate the carcinogenecity of nitrous acid ammonia.Therefore become the core of little ecological heat for the research of probioticss such as bacillus bifidus.

Bacillus acidophilus's physiological action is only second to bacillus bifidus.This bacterium is normal definite value in intestinal, and is harmless and useful, the energy synthetic vitamin, and assist digestion food helps alimentation, promotes organism metabolism.This bacterium produces acid in intestinal, collaborative bacillus bifidus suppresses harmful bacterium, overcomes decay process, reduces the generation of harmful substance and quickens the eliminating of harmful substance.This bacterium can also reduce the body cholesterol, and enhancing body is to the endurance of lactic acid.Through the yoghourt of bacillus acidophilus's fermentation, human edible T﹠B, well received, this also illustrates the benefit of this bacterium to the mankind.The bacteriocin that the bacillus acidophilus produces has the obvious suppression effect to lactobacillus, bright string coccus, Lactococcus and streptococcus thermophilus.Honso (1977) has reported the bacteriocin that the bacillus acidophilus produces, and it is synthetic to suppress colibacillary DNA (deoxyribonucleic acid) (DNA).Ministry of Health of the People's Republic of China issues " the probiotic bacteria strain list that can be used for health food " (defending the method prison sends out [2001] No. 84) on April calendar year 2001 9 wherein just bacillus bifidus and bacillus acidophilus.

Whether healthy bacillus bifidus quantity become check human body index.It can reduce the physiological action of human body:

1. antibiotic property, its antibacterial action mechanism produces organic acid and causes intestinal pH to descend suppressing putrefaction bacteria for decomposing saccharide, and this bacteria growing also can produce a kind of antibiotic substance (bifdin) to certain phase simultaneously also has inhibitory action to saprophytic bacteria; Can make the coupling bile acid be decomposed into free bile acid, this acid is stronger to bacteriostasis, and after saprophytic bacteria was suppressed, harmful substances such as the intravital indole of people, phenol, ammonia and cadaverine also obviously reduced.

2. replenish the vitamin of needed by human body.Bacillus bifidus can produce various vitamin as: VB1, VB2, VB6, VB12, nicotinic acid and folic acid etc. can also decompose the supply that ensures vitamin by suppressing some vitamin for needed by human body.

3. the anti-effect that treats constipation.The organic acid that the bacillus bifidus metabolism is produced can make the interior osmotic pressure of intestinal tube increase, and moisture increases in the feces, can promote the intestinal tube wriggling simultaneously, so can prevent constipation.

4. liver function is improved.Saprophytic bacteria can produce metabolite such as a large amount of indole, hydrogen sulfide, amine in the intestinal, need liver detoxification, get rid of with forms such as glucuronic acid salt subsequently, if untimely detoxifcation will cause liver dysfunction and blood circulation is not normal, disturb nervous system and influence sleep, bacillus bifidus can absorb these nitrogen substances, suppresses to produce the putrefaction bacteria of amine.

5. raise immunity.Bacillus bifidus produces the intestinal immune cell to stimulate, and produces the effect that ability plays enhance immunity by improving intestinal immune globulin A (LgA) plasma cell.Bacillus bifidus and bacillus acidophilus's full cell or cell extract effect in this respect are very remarkable.

6. prevent intestinal canal tumour.The intestinal saprophytic bacteria can produce many amine carcinogen in metabolism, what have can also be converted into carcinogen with some carcinogenic precursor substances, bacillus bifidus by suppress saprophytic bacteria growth and decompose carcinogenic substances play the effect of prevention intestinal cancer.

The active bacteria formulation quantity of present bacillus bifidus both domestic and external is a lot, but finding in actual applications that these active bacteria formulations are used to adjust and improve the intestinal microecology balance also exists the limitation that existing science and technology is difficult to overcome: no matter 1. which kind of dosage form product must be through digestive tract especially gastric acid, thereby be easy to lose activity.2. by survival rate behind the gastric acid barrier and the field planting problem that reaches intestinal.The short biology of probiotics is the another kind of approach of adjusting intestinal microecology, at present this research is on the one hand concentrated on the oligosaccharide that is called " bifidus factor ", but shortcoming such as the prior function oligosaccharide all exists, and efficacy factor is indeterminate, complicated component, onset dosage are big, and can not breed the bacillus acidophilus, thereby effect is relatively poor.

Stachyose (Stachyose) is a kind of novel functional oligose, and the stachyose that processing is extracted can contain Raffinose usually.Stachyose and Raffinose all are functional oligoses, it all is the multiplicaiton factor of bacillus bifidus, the oligosaccharide that stachyose and Raffinose all are made up of galactose, glucose, fructose, connect 1 galactose (formation Raffinose) and 2 galactose (formation stachyose) in glucosyl group one side with α-(1,6) glycosidic bond in the molecule.Because lack the enzyme α-D-Ban Rutangganmei of hydrolysis water threose and Raffinose in the human body, so they can not be absorbed by human consumption, the intravital most of flora of people does not have corresponding enzyme system yet, therefore can not utilize, only residing in minority probioticss such as bacillus bifidus in the intestinal and bacillus acidophilus has this enzyme system, so stachyose has just become the specificity of these probioticss short biological with Raffinose.The stachyose efficacy factor is clear and definite, and structure is clear, has well remedied the defective of existing product aspect quality and effect.Be described as third generation functional oligose, have good prospects.

Summary of the invention the objective of the invention is to third generation functional oligose-stachyose that utilization structure is clear, efficacy factor is clear and definite, be used as the quick increment factor of these two kinds of probioticss of bacillus bifidus and bacillus acidophilus, significantly improve the quantity of these two kinds of probioticss in the intestinal.And quick active, field planting, propagation, reach and adjust and improve the equilibrated purpose of intestinal microecology.Thereby human body immunity improving reason, slow down aging, inhibition tumor, blood lipid regulation, improve functions such as gastrointestinal.

A kind of intestinal microecology regulator of the present invention mainly contains bacillus bifidus, bacillus acidophilus and stachyose, and each composition accounts for weight ratio and is:

Stachyose 10%-90%

Raffinose 1%-13.5%

Mycopowder 5%-75%

Additive 0-30%

The used stachyose of the present invention means highly purified stachyose product, can contain a small amount of Raffinose, and purity is greater than 40%.

Mycopowder of the present invention is bacillus bifidus and bacillus acidophilus's a mixture, both ratios are 1%-99%, can be the extract of viable bacteria, viable bacteria lyophilized powder, dead bacterium or somatic cells, strain comprises people source type, wild type and the variant that goes down to posterity, tame etc.Bacillus bifidus comprises bifidobacteria infantis (B.infants), bifidobacterium longum (B.longum), bifidobacterium breve (B.breve), bifidobacterium bifidum (B.bifidum), bifidobacterium adolescentis (B.adolessentis) etc.;

Additive of the present invention can be various edible essences, binding agent, disintegrating agent, fruit essence and sugar etc.The present invention can be tablet, electuary, capsule, oral liquid or other peroral dosage form.

Through carrying out the experiment of in-vitro multiplication effect, we find that stachyose and cottonseed sugar all have tangible proliferation function to bacillus bifidus.In order to estimate the safety of this intestinal microecology regulator, carried out mice LD ₅₀, bone marrow micronucleus, sperm distortion and Salmonella reversion test, prove to the invention belongs to actual nontoxic level material that somatic cell is not had mutagenesis, sexual cell is not had the mutation teratogenesis, no direct or indirect mutagenic action.Referring to table 1-4.

Table 1 The acute toxicity tests

Dosage (g/kg)	Number of animals (only)		The animal dead number
					Female	Male	Female	Male
	2.15 4.65 10.00 21.50	5 5 5 5	5 5 5 5	0 0 0 0					0 0 0 0

Annotate: Horn ' s method: animal: Wistar rat; Route of administration: irritate stomach LD ₅₀＞21.50g/kg.

Table 2 mouse bone marrow cells micronucleus test

Dosage (g/kg)	Number of animals (only)		Examined polychromatic erythrocyte number (individual)	Micronucleus number (individual)	Micronuclear rates (‰)
						Female	Male
	0.00 0.68 1.35 2.70 5.40 10.75 ring mebenil (0.06)	5 5 5 5 5 5 5						5 5 5 5 5 5 5	10000 10000 10000 10000 10000 10000 10000	14 15 16 19 14 15 249	1.4 1.5 1.6 1.9 1.4 1.9 24.9**

Annotate: the positive group of several dosage groups of * * blank group and cyclophosphamide compares P＜0.01 result: somatic cell is not had mutagenesis.

The influence of table 3 mouse sperm deformity rate

Dosage (g/kg)	Number of animals (only)	Examined sperm count (bar)	Teratospermia number (bar)	Abnormal rate (‰)
					Negative control group 0.85 8.5 17.0 cyclophosphamide	5 .5 .5 .5 5	5000 5000 5000 5000 5000	99 79 105 127 364	1.98±0.44 1.58±0.68 2.10±0.42 2.54±0.25 72.3**

Annotate: * * presses Wincoxon sequential test result, and blank and each dosage group and cyclophosphamide group compare, P＜0.01.

The result: recipe ingredient is not found the effect of mutation sperm deformity during up to the 17.0g/kg body weight yet.

The dull and stereotyped infiltration method testing result of table 4 (Salmonella reversion test)

Tried thing ug/ ware	TA97		TA98		TA100		TA102
									-S9	+S9	-S9	+S9	-S9	+S9	-S9	+S9
	Naturally return and become 0.5 5 50 500 5000 positive control Dexon Sodium azide MMC 2-AF	177 .152 144 147 162 168 ＞1000	167 171 162 157 172 ＞1000	36 30 28 32 27 23 ＞1000	31 29 33 32 29 ＞2000	193 156 170 160 162 159 ＞1500	173 182 163 167 167 ＞1500	302 293 272 289 316 270 ＞1500									312 329 314 317 306 580±10

Annotate: 1.TA97, TA102 do not add positive thing that S9 and TA102 add S9 with fenaminosulf 50ug/ ware.

2.TA97, TA98, TA100 add the positive useless 2-aminofluorene 5ug/ ware of S9.

3.TA100 do not add the positive thing of S9 sodium azide 1.5ug/ ware.

The result: no matter this prescription adds or does not add the S9 mixture, does not all find direct or indirect mutagenic action.

In sum, a kind of intestinal microecology regulator has its original feature, through the online information retrieval in pertinent literature storehouse both at home and abroad, does not see identical laboratory report, research report, fills a prescription and introduce and functional evaluation.Illustrate that this prescription has advance and practicality aspect treatment and the health care.

Concrete embodiment

Embodiment 1:

Stachyose 80% cottonseed sugar 3%

Bacillus bifidus powder 10% additive 7%

Embodiment 2:

Stachyose 84% cottonseed sugar 5%

Bacillus acidophilus's powder 6% additive 5%

Embodiment 3

Stachyose 79% cottonseed sugar 6%

Bacillus bifidus powder 4% bacillus acidophilus's powder 6%

Additive 5%

Embodiment 4

Stachyose 50% cottonseed sugar 7%

The dead full cell 20% bacillus acidophilus's powder 15% of bacillus bifidus

Additive 8%

Embodiment 5

Stachyose 63% cottonseed sugar 2%

The full cell 25% of bacillus bifidus powder 10% dead bacillus acidophilus

Embodiment 6

Stachyose 57% cottonseed sugar 4%

Bacillus bifidus powder 10% bacillus acidophilus's extract 20%

Additive 9%

Embodiment 7

Stachyose 64% cottonseed sugar 1%

Bacillus bifidus extract 20% bacillus acidophilus's powder 10%

Additive 5%

Embodiment 8

Stachyose 62% cottonseed sugar 1%

Bacillus bifidus extract 20% bacillus acidophilus's extract 15%

Additive 2%

Embodiment 9

Stachyose 57% cottonseed sugar 1%

The dead full cell 20% of the full cell 20% dead bacillus acidophilus of bacillus bifidus

Additive 2%

Claims (4)

1, a kind of intestinal microecology regulator is characterized in that: each composition of this regulator accounts for weight ratio and is:

Stachyose 80% cottonseed sugar 3%

Bacillus bifidus powder 10% additive 7%.

2, a kind of intestinal microecology regulator is characterized in that: each composition of this regulator accounts for weight ratio and is:

Stachyose 84% cottonseed sugar 5%

Bacillus acidophilus's powder 6% additive 5%.

3, a kind of intestinal microecology regulator is characterized in that: each composition of this regulator accounts for weight ratio and is:

Stachyose 79% cottonseed sugar 6%

Bacillus bifidus powder 4% bacillus acidophilus's powder 6%

Additive 5%.

4, a kind of intestinal microecology regulator is characterized in that: each composition of this regulator accounts for weight ratio and is:

Stachyose 57% cottonseed sugar 1%

The dead full cell 20% of the full cell 20% dead bacillus acidophilus of bacillus bifidus

Additive 2%.