CN1271945C - Composition of ganglioside and bean food and its application - Google Patents
Composition of ganglioside and bean food and its application Download PDFInfo
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Abstract
The present invention discloses a composition of ganglioside and bean food and an application thereof on aspects of delaying aging and improving memory. In the present invention, main excellent nutrient components of the ganglioside and the bean food are collected and mixed according to a modern health theory of medicine and food isogeny, and bean food capable of delaying aging and improving memory is provided for people, and the bean food is used as daily health food of people, and contains ganglioside. Test results indicate that the composition has strong functions of reducing lipid peroxidation, the life of male fruit flies and female fruit flies who eat the composition is obviously prolonged, and the learning times and correct reaction memory retention conditions of normal Wistar rats in bidirectional avoidance tests of shuttle boxes are obviously improved. The composition has obvious reminiscence functions on memory disorder models. The composition has no toxicity, and is safe to eat.
Description
Technical field
The present invention relates to a kind of bean food, relate in particular to the composition and the application thereof of gangliosides (English name Ganglioside) and bean food.
Background technology
Bean food industry can be divided into three major types: the one, and traditional soybean product comprises non-fermented product and fermented products such as soy sauce, fermented bean curd such as bean curd, dried bean curd; The 2nd, emerging bean product comprise protein products such as full-fat bean goods such as soymilk powder, soymilk and protein isolate, protein concentrate, histone, protein beverage, and oil product; The 3rd, beans alimentary health-care function composition development and use goods, the product that normally utilizes solvent extraction, resin separation purification to prepare comprises goods such as soybean lecithin goods, soyabean oligosaccharides, isoflavones, soyasaponins, fibre and soya.
No matter the processing technology of bean food how, its contained nutrition composition is along with the development of modern science and technology is cracked one by one.Bean food does not contain cholesterol, lactose and contains unrighted acids such as a large amount of linoleic acid, leukotrienes and soybean protein, soybean lecithin, isoflavones, soyasaponins, fibre and soya, and more lysine, vitamin E, lecithin, and mineral element such as calcium, iron, be called " brain tonic " food by the west nutritionist.Main nutrition composition, effect and the mechanism of bean food see Table 1.
The main component of table 1 bean food, effect and mechanism
| Main nutrient composition | Main effect | Related Mechanism |
| Soybean lecithin | Reducing blood lipid; The prevention cardiovascular and cerebrovascular disease | Cholesterol deposits in cardiovascular and cerebrovascular, cause the vascular wall hardening, brain and lumen of vessels narrow down, and vascular wall follows the string, and easily break, embolism.The deposition of cholesterol mainly is because phosphatide and cholesterol out of proportion in the body.Phosphatide can be transported to nutritional labeling each position of human body in blood, shortened the holdup time of fat in human body.Reduce content of cholesterol thus, hindered the deposition of cholesterol, thereby played the effect of reducing blood lipid. |
| Delay senility | Soybean lecithin contains a large amount of unrighted acids, can improve the hardenability of cell membrane, delay the aging of cell, thereby play antidotal effect. | |
| Improve neurochemistry function and cerebral function, activate brain cell, improve man memory power and notice | Phosphatide is to form brain cell and the requisite composition of nerve cell.The metabolism of phosphatide is relevant with the functional state of brain in the organism.The people is after taking soybean lecithin, generate choline, phosphoglycerol and aliphatic acid through hydrolysis in the body, have stronger physiologically active and nutritive value | |
| Soyasaponins | Improve and prevention of arterial sclerosis, hypertension, heart disease and headstroke. | Soyasaponins is a kind of good emulsifying agent, cholesterol and neutral fat emulsification in the blood vessel are discharged, reduce the content of blood cholesterol and triglycerides, prevent that it is deposited in the arteries |
| Anti-oxidant, Green Tea Extract, reduction lipid peroxide | Soyasaponins has the oxidation of lipotropism matter and increases the effect of superoxide dismutase (SOD) content, reduce the damage of lipid peroxide pair cell, remove free radical. | |
| Suppress decrease of platelet | Anti thrombotic action | |
| Suppress tumor growth. | Soyasaponins directly destroys the structure of tumor cell membrane or suppresses the synthetic of DNA. | |
| Antiviral | Soyasaponins has the ability of broad-spectrum antiviral, is to DNA or RNA virus all has obvious effect, has wide application prospect. | |
| Delaying cell aging | Participate in immunological regulation | |
| Isoflavones | The prevention menopausal syndrome; Osteoporosis | The mechanism generation effect of competing mutually as phytoestrogen and mammal estrogen |
| Inhibin of protease; Phytase; Phytosterol; Saponin; Isoflavones | Anticancer | Phytase, cellulose can prevent colon cancer; Inhibin of protease can suppress cutaneum carcinoma, carcinoma of urinary bladder, breast cancer, also can bring into play the inhibition effect to colon cancer, lung cancer, cancer of pancreas, carcinoma of mouth; Isoflavones can prevent breast cancer and prostate cancer; Phytosterol can suppress colon cancer. |
| Soyabean oligosaccharides | The coordinating intestines and stomach function | Promote the growth of Bifidobacterium in the human body, it is long fundamentally to suppress the intestinal toxic bacterium all living creatures, prevents treating constipation and suffering from diarrhoea. |
| Soybean nucleic acid | Improve brain function and immunity, the anti-infection | The core substance of cell strengthens autonomous repair ability, stimulates circulation |
The method that often adopting at present adds up strengthens is developed the bean food of a lot of enriched nutritives.Many patent disclosures be food, health food and drug component and the technology of preparing thereof that primary raw material is made with beans or beans extract.As Chinese patent 94118442, " Chinese wolfberry soyabean milk powder and production method thereof " disclosed; Chinese patent 94111764 discloses " a kind of prescription of multivitamin soybean milk and production method thereof "; Chinese patent 95106995 discloses " health care for the middle and old aged soymilk powder and method for making thereof "; Chinese patent 98125243 discloses " sugar-reducing pumpkin bean milk powder "; Chinese patent 00129720 discloses " instant low-sugar bean milk powder and preparation method thereof "; Milan, ITA patent 98807664 discloses " extract of soybean, its preparation method and pharmaceutical composition thereof ".
In the disclosed various bean foods of above-mentioned patent, do not see that a kind of bean food that contains gangliosides openly reports.
Gangliosides are a kind of sialic glycosphingolipids that contain, and mainly are present in the mammal ectocinerea, are the characteristic fat compositions of some neuron film of central nervous system.The gangliosides molecule is made up of a hydrophilic radical (sialic acid compound sugar) and a lipophilic group (acyl sphingosine).Lipophilic group embeds two lipid layers of neuron membrane, and hydrophilic radical protrudes in extracellular fluid, helps to keep to the full extent negatively charged cell surface.This asymmetry and chemical constitution physically interacts the various information in gangliosides material and extracellular, and can cause the change of part membrane structure.Mainly contain Monostalotetrahexosylgangliside (GM in vivo
1), a sialic acid tetrahexose gangliosides (GD
1a), two sialic acid tetrahexose gangliosides (GD
1b), GT1b (GT
1b) and GM
3, GD
3, GQ
1bEtc. form.Fusing point: 190 ℃; Solubility: easily be dissolved in water, ethanol.
Gangliosides physiologic function: 1. promote substantia nigra dopaminergic neuron (nigral dopaminergicneuron, NDN) the living pleurapophysis growth of tooth; 2. promote wound repair: it is damaged that gangliosides can directly infiltrate in the cell membrane filled film, and excite the activity of repairing damage widely by information transfering action; 3. promote functional rehabilitation: it is synthetic that gangliosides have the dopamine of promotion, quickens effects such as axoplasm transportation, helps the recovery of nervous function; 4. to Green Tea Extract: gangliosides can be protected the cell membrane unrighted acid and improve membrane stability, and reduce the radical pair cells injury.(Chin J Neurol，August 1997，Vol 30，4；208-210)。
The technology of from animal tissue, extracting gangliosides of existing maturation and the further technology of separation and purification at present, GM wherein
1As the existing commercially available prod of medicine, its commodity are by name: GM-1, and single sialic acid four is the sugar ganglioside sodium-salt parenteral solution, import registration certificate x19990444, x19990445, manufacturer: Argentinian TRB pharmaceutical factory trb pharma s.a.; Patent 19901117 discloses " not by the preparation and the purification process of the sugared sphingolipid mixture of unconventional viral pollution "; Chinese patent 95111569 discloses " a kind of contain the production method that gangliosides are main glycolipid class material ".
Many in the world developed countries, area and city enter " aging ".According to statistics, the big and medium-sized cities ages such as Beijing, Shanghai, Tianjin, Nanjing surpass 17% the old man more than 60 years old.And human brain is old and feeble at first position (may work as you began in the time of 30 years old).Medical research shows, the main cause that causes aging of brain be do not require mental skill, cranial nerve cell is aging, diseases such as not enough, trouble artery sclerosis of good storages and hypertension in the brain.Therefore the food that delays senility, improves memory will be the main flow of 21st century food industries, and all at present bean foods all do not have delaying senility, improve memory impairments, can not satisfy people's demand.
Summary of the invention
One of technical issues that need to address of the present invention provide the composition of a kind of gangliosides and bean food, to overcome the defective in existing product field, satisfy people's demand;
Two of the technical issues that need to address of the present invention are to disclose the application of above-mentioned bean food in delaying senility function;
Three of the technical issues that need to address of the present invention are to disclose the application of above-mentioned bean food in improving memory effect.
The present invention is according to the modern health theory of doctor's food homology, with this promotion neurotrophy of gangliosides, growth and reparation simultaneously to the distinguished natural extraction composition of Green Tea Extract effect and the main nutrition composition of bean food (as soybean lecithin, soyasaponins, soybean nucleic acid, soybean protein, vitamin etc., see Table 1) collect excellent mixing, (the excellent mixing of so-called collection, be meant a kind of like this combined innovation, promptly select the advantage key element of several things, mix or the chemistry mixing through physical property then, its objective is that the more single things of acquisition has more the new things of characteristic or advantage.) a kind of delaying sanility is provided and improves the daily health caring food of the bean food that contains gangliosides of memory as people.
Main nutrition composition of bean food and gangliosides have synergy: 1. phosphatide and gangliosides all are the constituents of cell membrane.Phosphatide is being born the function that the supply cell earns a bare living necessary material and drains refuse in the metabolic process, the people is after taking soybean lecithin, generate choline, phosphoglycerol and aliphatic acid through hydrolysis in the body, can improve the hardenability of cell membrane, delay the aging of cell, and gangliosides can be protected the cell membrane unrighted acid and improve membrane stability, and reduce the radical pair cells injury; 2. soyabean oligosaccharides promotes the growth of intestinal bifidobacteria, and Bifidobacterium can " catch " gangliosides, improves the absorption of gangliosides at enteron aisle; 3. soyasaponins has the effect of oxidation of lipotropism matter and increase superoxide dismutase (SOD) content, reduce the damage of lipid peroxide pair cell, remove free radical, guarantee that the external source gangliosides can steadily enter cell membrane, the activity of the sodium-potassium-ATP enzyme on the active cell film participates in the transmission of cell information simultaneously; 4. soybean nucleic acid is the core substance of cell, can stimulate circulation after human body is edible, strengthens the autonomous repair ability of cell, and in the presence of materials such as gangliosides, the better impaired and dead brain cell of repair and reconstruction improves brain function and metabolism.Gangliosides are added in the bean food by certain proportioning, not only keep the nutritive value of gangliosides and bean food itself, simultaneously various nutritional labelings are complemented each other, be more conducive to body absorbing gangliosides; In addition, bean food as people's daily bread, can cater to the needs of each age groups.Therefore provide a kind of brain and neurodevelopment, memory are played an important role, the bean food that contains gangliosides that has delaying senility and improve memory effect is very necessary.
The composition of gangliosides of the present invention and bean food comprises bean food and gangliosides.
Wherein: the weight percent content of gangliosides counts 2～10% with the nutrition composition in the bean food, and the bean food nutrition composition is 90～98%.
Said bean food comprises soya-bean milk, soymilk, instant bean or the soyabean protein powder etc. that adopt techniques well known to be prepared from, or adopts the commercially available prod, as the product soya-bean milk of beneficial Kang Bao Food Co., Ltd, soymilk, instant bean, soyabean protein powder etc.
Said bean food nutrition composition comprises one or more in soybean protein, soybean lecithin, soyasaponins, isoflavones, soybean nucleic acid, soyabean oligosaccharides, each biostearin, mineral matter or the phytosterol etc.
Preferred content is:
Gangliosides 0.01～10.00%
Bean food nutrition composition 90.00～99.99%.
More preferably content is:
Gangliosides 10.01～40.00%
Bean food nutrition composition 60.00～89.99%.
More preferably content is:
Gangliosides 40.01～80.00%
Bean food nutrition composition 20.00～59.99%.
More preferably content is:
Gangliosides 80.01～99.99%
Bean food nutrition composition 0.01～19.99%.
Most preferred content is:
Gangliosides 1.00～10.00%
Bean food nutrition composition 99.00～90.00%.
Said ratio is based on varieties of food items in the bean food industry, does not contain other additive, can add acceptable stabilizing agent, essence etc. in the food industry when reality is used.
The analytical method of soybean lecithin, soyasaponins, isoflavones, soybean nucleic acid, soyabean oligosaccharides, soybean protein or phytosterol can be consulted document " food composition analysis handbook, China Light Industry Press.
Above-mentioned gangliosides adopt " a kind of separation and purification prepares neuromere fat technology " application number: 85102590, disclosed technology is prepared, or employing commercially available prod, the specification of producing as precious intelligence biological products Co., Ltd is that 10 kilograms model is the thick product of gangliosides of Y/G2001-5, carries out purifying with the method for this laboratory foundation again.
The composition of gangliosides of the present invention and bean food can be used for delaying senility;
The composition of gangliosides of the present invention and bean food also can be used for improving memory.
In order to understand essence of the present invention better, below will content of the present invention be described with preparation, foodsafety (toxicity) test and function test and the result of this bean food.
The gangliosides that the present invention adopts are to provide thick product by precious intelligence biological products Co., Ltd, and use this breadboard purification technique and obtain highly purified gangliosides.Concrete technology is as follows:
Thick product concentrates in 40～60 ℃, and the acetone precipitation gangliosides with 5～10 times of amounts discard acetone, are being lower than vacuum drying under 60 ℃ of temperature.
The bean food that the present invention adopts is available from the product soya-bean milk of beneficial Kang Bao Food Co., Ltd, soymilk, instant bean, soyabean protein powder etc.
Adopt conventional method that above-mentioned two kinds of main raw materials in proportion are made soymilk and instant bean, it is as follows to fill a prescription:
Soymilk: gangliosides 5.00%
Bean food nutrition composition 95.00%
(wherein the bean food nutrition composition comprises: soybean protein 38.00%, soybean lecithin 13.25%, isoflavones 11.00%, soyasaponins 0.85%, soyabean oligosaccharides 3.11%, phytosterol 6.75%, soybean nucleic acid 9.15%, other 12.89%).
Instant bean: gangliosides 5.00%
Bean food nutrition composition 95.00%
(wherein the bean food nutrition composition comprises: soybean protein 35.00%, soybean lecithin 15.27%, isoflavones 10.00%, soyasaponins 1.50%, soyabean oligosaccharides 3.89%, phytosterol 7.49%, soybean nucleic acid 8.85%, other 13.00%).
The experimental result division is as follows:
One, foodsafety toxicological experiment
1. materials and methods
40 22 monthly age male Wistar rats (Military Medical Science Institute's animal center provides), body weight (450 ± 40) g is divided into 4 groups, and gangliosides divide three groups by 10mg/kg, 1.0mg/kg, 0.1mg/kg prescription soymilk formulation, feed continuously 60 days.
2. result
Taking this prescription soymilk group rat does not have death, and another group compares with common soymilk; It is all normal respectively to organize body weight situation, hematology and biochemistry detection, organ weights and histological examination, and the result shows that taking group does not have significant difference with control group.Organs and tissues is learned check pathological section and is finished by pathology teaching and research group of Shanghai Second Emdical University.
The above results illustrates, contains the soymilk avirulence of gangliosides, edible safety.
Two, functional experiment
Delay senility
A, zoopery
1. materials and methods
(1) tried thing:
1. the prescription soymilk among the embodiment 2 is evaporated to 5mg gangliosides/ml through 60～70 ℃; 2. common soymilk, 200ml is evaporated to 1ml through 60～70 ℃; 3. the pure water that contains gangliosides, 5mg gangliosides/ml.
(2) animal used as test and grouping:
Body weight is 77 of the Kunming kind female mices of 22.7 ± 3.8g, and institute of biological products, Sichuan Province Experimental Animal Center provides the (quality certification number: the real moving pipe matter in river 98-6 number).Kunming mouse is carried out after all adaptability feeds, and be divided at random: 1.7ml/kg group, 3.3ml/kg group, 5.0ml/kg organizes three dosage groups, aging model control group, normal control group, common soymilk group and gangliosides group merely.Every group of 11 animals.
(3) experimental technique and testing index
Three dosage groups, common soymilk group, simple gangliosides group (general designation experimental group) and the dosage nape hypodermic injection 0.2%D-galactose solution that the aging model mice in control group is pressed 40mg/kg every day, meanwhile the experimental group difference of irritating the stomach corresponding dosage is respectively tried thing, and the aging model group is then irritated stomach distilled water; Normal control group nape every day hypodermic injection sterile saline 0.5ml, and irritate stomach distilled water.After continuous 40 days, slaughter animal and get blood and utilize kit measurement serum MDA (MDA) content and superoxide dismutase (SOD) activity.
2. result
(1) the aging model control group is compared with the normal control group:
SOD active significantly reduction (P＜0.05), MDA reserves significantly increase (P＜0.01), see Table two.Illustrate that the subacute aging model of animal sets up.
(2) Content of MDA
As shown in Table 2, the MDA content of 1. organizing three dosage groups significantly is lower than aging model control group (P＜0.01); 2. common soymilk group, 3. simple gangliosides group content is lower than aging model control group (P＜0.05); 2., 3. the group with 1. the group there were significant differences (P＜0.05); The normal control group is compared no difference of science of statistics (P＞0.05).See Table 2.
(3) activity of SOD in serum
It is as shown in the table, and the SOD activity of 3.3ml/kg group and 5.0ml/kg group is significantly higher than aging model control group (P＜0.01), the active and relatively more equal no difference of science of statistics (P＞0.05) of normal control group of the SOD of three dosage groups; 2. common soymilk group, SOD activity are higher than aging model control group (P＜0.05), with 1. the group there were significant differences (P＜0.05); 3. the active and aging model control group no significant difference (P＞0.05) of simple gangliosides group SOD.See Table 2.
The influence of table 2 couple mice serum MDA, SOD activity (X ± S)
| Group | The group number | MDA content (X ± S) | The SOD activity (X ± S) | |
| 1. dosage group | 1.7ml/kg | 11 | 5.8±0.5 | 359.63±56.95 |
| 3.3ml/kg | 11 | 5.5±0.9 | 360.24±41.11 | |
| 5.0ml/kg | 11 | 5.0±1.0 | 363.17±50.33 | |
| 2. common soymilk | 11 | 7.8±0.9 | 357.67±40.65 | |
| 3. simple gangliosides | 11 | 7.5±1.1 | 355.32±50.91 | |
| The aging model group | 11 | 12.6±0.3 | 341.63±49.37 | |
| The normal control group | 11 | 5.2±0.8 | 360.01±39.88 | |
MDA content, 1. the dosage group is compared P＜0.01 with the aging model group, and 2., 3. group is compared P＜0.05,2., 3. group is compared P＞0.05;
The SOD activity, 1. the dosage group is compared P＜0.01 with the aging model group, the SOD activity of 3.3ml/kg group and 5.0ml/kg group with 2., 3. group is compared P＜0.05,2., 3. organize and compare P＞0.05.
3. conclusion
The soymilk that contains gangliosides has more the effect that reduces lipid peroxidation, increased SOD, the result of the test positive than the gangliosides and the soya milk product of its single nutrition composition.
B, delaying senility function---drosophila survival test
1. materials and methods
(1) fruit bat: adopt the wild type Drosophila melanogaster, provide by biology department of Sichuan University.
(2) basal feed: corn flour 12g, sucrose 9g, agar 1.5g, dusty yeast 1g, benzoic acid 0.2g, water 110ml.
(3) tried thing:
1. the prescription soymilk among the embodiment 2 is evaporated to 5mg gangliosides/ml through 60～70 ℃;
2. common soymilk, 200ml is evaporated to 1ml through 60～70 ℃;
3. the pure water that contains gangliosides, 5mg gangliosides/ml;
Other establishes a blank group (basal feed).About 200 of every group of female male drosophila.
(5) existence test
Collect the one-tenth fly that hatches in the 10h, weigh by male and female behind the etherization, and to be sub-packed in be that 3.0cm * 13cm cultivates in the vial.Fruit bat is raised in 25 ℃ ± 2 ℃ dark incubators, and every 4d changes feed once.Fruit bat begins the above-mentioned experimental group of feeding after hatching for two weeks, and every day time recording fruit bat death toll, until whole death.
(6) statistical analysis
The relatively employing t check of fruit bat average life span and maximum life span: the relatively employing X2 check of half death time (median).
2. result
The half death time, average life span and the life-span that contain the male and female fruit bat of the soymilk of gangliosides be all greater than control group, and than the gangliosides and the soya milk product more remarkable effect of its single nutrition composition, see Table 3.
The influence of table 3 pair life span of drosophila melanogaster (X ± S)
| Group | The group number | Average weight (μ g) | The half death time (d) | Average life span (d) | Maximum life span (d) | |
| Female | Contrast | 191 | 600±21 | 62.9 | 60.4±11.1 | 78.6±2.6 |
| ① | 204 | 578±84 | 70.2 | 68.6±12.8 | 89.7±2.8 | |
| ② | 224 | 608±48 | 63.9 | 62.3±11.2 | 80.8±2.5 | |
| ③ | 185 | 580±31 | 64.8 | 62.5±11.8 | 81.3±1.5 | |
| Male | Contrast | 192 | 571±51 | 66.8 | 65.0±12.1 | 83.8±2.3 |
| ① | 185 | 723±31 | 73.5 | 71.9±15.1 | 90.4±3.5 | |
| ② | 216 | 600±82 | 69.8 | 66.6±17.0 | 85.2±1.9 | |
| ③ | 192 | 701±96 | 68.2 | 69.0±11.9 | 86.7±1.0 | |
1. and 2., 3. group P＜0.05 relatively with control group; 2., 3. group is compared P＞0.05.
3. conclusion
The drosophila survival result of the test shows that the soymilk half death time, average life span and the maximum life span male and female fruit bat that contain gangliosides all obviously prolong, and promptly this drosophila survival result of the test of being tried thing is positive; And gangliosides and soya milk product more remarkable effect than its single nutrition composition.
One) improves memory
A, animal shuttle box test (two-way avoidance test)
1. materials and methods
(1) material
Animal used as test: the Wistar rat, adult rats (male, female all have), body weight 18～22g is divided into 12 groups, 30 every group.
Instrument: the rat shuttle box, this device is made up of experimental box and automatic record printing equipment.
Reagent: anisodine, cycloheximide.
Tried thing and dosage: the 1. instant bean among the embodiment 3, stick with paste with hot drink water furnishing, making becomes 5mg gangliosides/ml; 2. common instant bean is stuck with paste with hot drink water furnishing; 3. the gelatinized corn starch that contains gangliosides, 5mg gangliosides/ml; Control group replaces with basal feed; Take the photograph people's amount according to human body per kilogram of body weight every day of recommending, enlarge 10 times and took continuously 30 days.
(2) experimental technique:
1) tried the influence that thing is set up the normal rat conditioned reflex
The Wistar rat is put any side in people's case, begins to present light or buzz behind the 20s, continue 20s, give simultaneously in the back 10s electro photoluminescence (100v, 0.2mA, 50Hz, AC).Rat promptly escapes after being shocked by electricity, must go to the offside top, just can interrupt electric shock after blocking photoelectric tube, this is a passive avoidance response, before each electric shock, give conditional stimulus, after strengthening repeatedly, rat promptly jumps to offside after acceptable conditions stimulates and blocks photoelectric tube and escape electric shock, and this is an active avoidance response.Stop to train in 5～50d, measure its memory situation that disappears.
2) tried the influence that thing is set up the conditioned reflex of dysmnesia model rat
The memory acquisition disturbance model is made: 10min lumbar injection anisodine or hyoscine 5mg/kg body weight before the training; The manufacturing of obstacle model is consolidated in memory: 10min lumbar injection actidione 120mg/kg body weight before the training; Method of operating is with above-mentioned 1).
3) data are handled and result's judgement
Animal is initiatively avoided the time and the passive avoidance time is used variance analysis; Test group active and/or passive avoidance time are significantly shorter than control group, and difference has conspicuousness (P≤0.05), judge that this experiment is positive, are decided to be association's standard.After reaching association, stop to train the mensuration of remembering the maintenance situation after 5 days again, record and statistical correction reaction percentage.
2. result
(1) study situation:
It is 33.2 ± 3.4 that normal Wistar rats reaches the required frequency of training of association's standard, and each group association desired times of experiment obviously is less than control group, P＜0.05; 2., 3. experiment 1. group is organized relatively with experiment, as a result P＜0.05; The experiment 2., 3. the group, P＞0.05, no significant difference sees Table four;
The memory acquisition disturbance model, control group association number of times 62.1 ± 5.7, apparently higher than the normal control group, model is set up successfully.Experiment 1. group association desired times obviously is less than model control group (P＜0.01), reaches normal level; 2., 3. experiment 1. group is organized relatively with experiment, as a result P＜0.05; 2., 3. experiment is organized with the normal control group and is compared P＞0.05, no significant difference; Compare with model control group, P＜0.05, there were significant differences, sees Table 4;
(2) stop to train 5 days after, memory maintenance situation:
The percentage of control group mice correct response is 32.2 ± 9.3; The percentage number average that correct response is respectively organized in experiment is higher than control group, statistical analysis P＜0.01; 2., 3. experiment 1. group is organized relatively with experiment, as a result P＜0.05; Experiment is 2., 3. group is compared, P＞0.05, and no significant difference sees Table 4;
The obstacle model is consolidated in memory, and control group correct response percentage 10.1 ± 9.8 (%) is starkly lower than the normal control group, and model is set up successfully.1. experiment organizes correct response percentage apparently higher than model control group (P＜0.01), reaches normal level; 2., 3. experiment 1. group is organized relatively with experiment, as a result P＜0.05; 2., 3. experiment is organized with the normal control group and is compared, P＜0.05, and there were significant differences; Compare with model control group, P＜0.05, there were significant differences, sees Table 4;
The influence of table 4 pair normal Wistar rats and dysmnesia model rat
| Group | The group number | Association's number of times (inferior) | Correct response percentage (%) | |
| Normal rat | Control group | 30 | 33.2±3.4 | 32.2±9.3 |
| Experiment is group 1. | 30 | 20.2±4.3 | 50.0±2.9 | |
| Experiment is group 2. | 30 | 27.1±3.3 | 45.1±9.9 | |
| Experiment is group 3. | 30 | 26.5±5.0 | 41.9±6.5 | |
| The memory acquisition disturbance model | Control group | 30 | 62.1±5.7 | 33.9±6.6 |
| Experiment is group 1. | 30 | 32.6±4.1 | 51.6±1.1 | |
| Experiment is group 2. | 30 | 48.6±6.4 | 43.1±9.8 | |
| Experiment is group 3. | 30 | 46.9±5.7 | 39.9±2.0 | |
| The obstacle model is consolidated in memory | Control group | 30 | 35.0±4.5 | 10.1±9.8 |
| Experiment is group 1. | 30 | 21.2±3.7 | 32.9±3.0 | |
| Experiment is group 2. | 30 | 26.1±8.6 | 22.3±6.8 | |
| Experiment is group 3. | 30 | 25.1±3.9 | 18.9±9.0 | |
1. experiment organizes normal Wistar rats and each model group, and association's number of times and correct response percentage are compared P＜0.01 with control group; With experiment 2., 3. group is compared P＜0.05;
Experiment 2., 3. group is learned number of times and correct response percentage, compares P＜0.05 with model control group; Compare P＜0.05 with the normal control group;
3. conclusion
The instant bean that contains gangliosides all has clear improvement to the association's number of times and the correct response memory maintenance situation of normal Wistar rats in the two-way avoidance test of shuttle box, and than the gangliosides and the instant bean more remarkable effect of its any single component;
Contain gangliosides and the instant bean of the instant bean of gangliosides, dysmnesia model is had more significant reminiscence effect than its any single component;
B, human feeding trial
1. material and method
(1) given the test agent: 1. contain the soymilk of gangliosides among the embodiment 2, contain gangliosides 5mg/200ml capsule; 2. common soymilk, the 200ml/ box; 3. the oral aqueous solution that contains gangliosides, gangliosides 5mg/10ml props up; Control group replaces with pure water; Took continuously 90 days.
(2) subjects: select healthy pupil, the mean age is 8 years old.
(3) the object grouping reaches to sample: be divided into experiment at random and 1., 2., 3. organize and control group, experimental group is respectively 32 people, and control group is 30 people.The upper and lower noon of every children every day takes once, presses package metro-measuring at every turn.
(4) test method: the human feeding trial rules of improving memory effect according to regulation in " the health food function assessment assessment process and the method for inspection " of Ministry of Public Health's promulgation in 1996 are carried out, and adopt Wechsler Memory Scale to press the memory quotient that test request is measured children.
(5) test scale adopts " Wechsler Memory Scale " revised edition of psychology teaching and research room of Hunan Medical University revision in 1989.
(6) data are handled and are adopted the t check relatively of pairing measurement data and the t check of two sample means.
2. result
(1) each experimental group is to the influence of children's test-meal front and back each minute test scale branch
Before the test-meal, experimental group and control group each minute test scale lease making statistical test, difference that there are no significant (P＞0.05); Test-meal is after statistical test, and each experiment component test scale score average all is higher than control group, and experience+orientation, picture memory, to carry on the back several three subtests be P＜0.01; Numerical order, understanding memory binomial subtest are P＜0.05.
(2) each subtest scale score compares before and after the experimental group test-meal
Each subtest scale score average is re-recognized except that vision after the experimental group test-meal, and outside the vision regeneration binomial subtest, all the other are every all highly difficult before test-meal, and experience+orientation, numerical order binomial are P＜0.01, and the memory binomial is recalled, understood to picture is P＜0.05.
(3) each subtest scale score compares before and after the control group test-meal
The result shows: remove the numeral order before and after the control group test-meal, and outside the back of the body number binomial subtest, all the other every subtest scale score means there are no significant difference.
(4) experimental group is to the influence of long-term memory, short-term memory, immediate memory before and after the test-meal.
A. to the influence of children's long-term memory
The long-term memory test comprises experience, orientation and three subtests of numerical order.Before the test-meal, the scale score no significant difference of experimental group and control group; And after the test-meal, the scale score of each experimental group all is higher than control group, and wherein 1. experiment is organized and 2., 3. organized (P＜0.05); 2., 3. the group between no significant difference.Control group scale score after test-meal also increases (38.07 ± 3.78 → 41.00 ± 1.11) to some extent, and 2., 3. organizing to compare to some extent with control group increases, but equal not statistically significants the results are shown in Table 5.
B. to the influence of children's short-term memory
Short-term memory comprises that vision is re-recognized, picture memory, vision regeneration, associative learning, touch test, understand six subtests of memory.Before the test-meal, the scale score no significant difference of experimental group and control group; And after the test-meal, the scale score of each experimental group all is higher than control group.
C. to the influence of children's immediate memory
The immediate memory test comprises along the back of the body and the back of the body numeral of falling.Before the test-meal, the scale score no significant difference of experimental group and control group; And after the test-meal, the scale score of each experimental group all is higher than control group.
(5) children are improved the effect of memory quotient
Experimental group is compared two groups of memory quotient levels before the test-meal with control group does not have significant difference (P＞0.05); 1. experiment is organized compared with the control after the test-meal, and there were significant differences for the memory quotient level (P＜0.01); 1. experiment is organized and is compared the memory quotient level before and after the test-meal significant difference (P＜0.01) is arranged; 2., 3. organize and compare the memory quotient level with control group after the test-meal and do not have significant difference (P＞0.05), the results are shown in Table 5.
The influence of table 5 pair long-term memory and memory quotient
| Group | The group number | Long-term memory | Memory quotient | ||
| Before taking | After taking | Before taking | After taking | ||
| Experiment is group 1. | 32 | 38.14±3.21 | 56.22±3.68 | 91.54±6.66 | 109.91±6.87 |
| Experiment is group 2. | 32 | 38.60±3.22 | 45.69±4.57 | 90.65±7.35 | 95.05±6.81 |
| Experiment is group 3. | 32 | 38.21±4.11 | 46.08±4.33 | 91.97±7.11 | 96.00±5.64 |
| Control group | 30 | 38.07±3.78 | 41.00±1.11 | 91.81±6.48 | 91.91±6.91 |
Experiment is group 1., and P＜0.01 is compared in P＜0.01 before and after long-term memory and memory quotient are taken with control group, 2., 3. organizes with experiment and compares, and takes back P＜0.05; 2., 3. organize after the test-meal and to compare P＞0.05 with control group.
3. conclusion:
The edible soymilk that contains gangliosides of normal child had obvious facilitation to long-term memory, short-term memory and immediate memory and memory quotient number average after 90 days, and its effect is more remarkable than the gangliosides and the soymilk of any single component.
By above-mentioned disclosed foodsafety toxicological test and function test and with the animal and human's body test-meal test relatively of its single component as seen:
1. the soymilk that contains gangliosides has more the effect that reduces lipid peroxidation, increased SOD than the gangliosides and the soya milk product of its single nutrition composition.
2. the drosophila survival result of the test shows, contain the soymilk of gangliosides half death time, average life span and maximum life span male and female fruit bat and all obviously prolong, and than the gangliosides and the soya milk product more remarkable effect of its single nutrition composition.
3. the instant bean that contains gangliosides, association number of times and the correct response memory maintenance situation of normal Wistar rats in the two-way avoidance test of shuttle box all had clear improvement, and than the gangliosides and the instant bean more remarkable effect of its any single component;
4. contain gangliosides and the instant bean of the instant bean of gangliosides, dysmnesia model is had more significant reminiscence effect than its any single component;
5. the edible soymilk that contains gangliosides of normal child had obvious facilitation to long-term memory, short-term memory and immediate memory and memory quotient number average after 90 days, and its effect is more remarkable than the gangliosides and the soymilk of any single component.
6. contain the soymilk avirulence of gangliosides, take safety.
The specific embodiment
Below will explain the specific embodiment of the present invention by embodiment.
Embodiment 1
The thick product of gangliosides that adopts precious intelligence biological products Co., Ltd to provide concentrates in 40～60 ℃, and the acetone precipitation gangliosides with 5 times of amounts discard acetone, are being lower than vacuum drying under 60 ℃ of temperature.
The bean food that adopts is soymilk, instant bean, soyabean protein powder, the soya-bean milk of beneficial Kang Bao Food Co., Ltd.
Embodiment 2
Is carrier by present technique field personnel's known method with the soymilk of beneficial Kang Bao Food Co., Ltd, with the gangliosides of embodiment 1 through stir, clean breast, homogeneous, pasteurization make with extra care and form.Its packing specification is that 200mL, 250mL, 500mL or 1L are box-packed, and the human body amount of drinking every day is no less than 200mL, and the proportioning of main component is:
Ganglioside fat raw material: 5 grams
Soymilk adds to: 1000 milliliters.
After tested, wherein soybean protein-containing 38.00 restrains, soybean lecithin 13.25 restrains, isoflavones 11.00 restrains, soyasaponins 0.85 restrains, soyabean oligosaccharides 3.11 restrains, phytosterol 6.75 restrains, soybean nucleic acid 9.15 restrains, other nutritional labelings 12.89 restrain.
Embodiment 3
Is carrier by present technique field personnel's known method with the instant bean of beneficial Kang Bao Food Co., Ltd, with the gangliosides of embodiment 1 mix, add acceptable flavor enhancement of field of food and stabilizing agent through ultra-fine grinding, with instant bean, sterilization is refining forms.The human body amount of drinking every day is no less than 10g, and the proportioning of main component is:
Ganglioside fat raw material: 5.0 grams
Instant bean adds to: 1000.0 grams.
After tested, wherein soybean protein-containing 35.00 restrains, soybean lecithin 15.27 restrains, isoflavones 10.00 restrains, soyasaponins 1.50 restrains, soyabean oligosaccharides 3.89 restrains, phytosterol 7.49 restrains, soybean nucleic acid 8.85 restrains, other nutritional labelings 13.00 restrain.
Embodiment 4
Is carrier by present technique field personnel's known method with the soya-bean milk of beneficial Kang Bao Food Co., Ltd, with the gangliosides of embodiment 1 through stir, clean breast, homogeneous, pasteurization make with extra care and form.Its packing specification is that 250mL, 500mL or 1L are box-packed, and the human body amount of drinking every day is no less than 250mL, and the proportioning of main component is:
Ganglioside fat raw material: 2.0 grams
Soya-bean milk adds to: 1000.0 milliliters
Wherein soybean protein-containing 38.00 restrains after tested, soybean lecithin 12.25 restrains, isoflavones 13.00 restrains, soyasaponins 0.85 restrains, soyabean oligosaccharides 2.57 restrains, phytosterol 6.75 restrains, soybean nucleic acid 9.15 restrains, other nutritional labelings 15.43 restrain.
Embodiment 5
Is carrier by present technique field personnel's known method with the soyabean protein powder of beneficial Kang Bao Food Co., Ltd, with the gangliosides of embodiment 1 mix, add acceptable flavor enhancement of field of food and stabilizing agent through ultra-fine grinding, with soyabean protein powder, sterilization is refining forms.Human body dose every day is no less than 10g, and the proportioning of main component is:
Ganglioside fat raw material: 10.0 grams
Soyabean protein powder adds to: 1000.0 grams.
Wherein soybean protein-containing 45.00 restrains after tested, soybean lecithin 12.25 restrains, isoflavones 11.00 restrains, soyasaponins 0.80 restrains, soyabean oligosaccharides 2.89 restrains, phytosterol 5.75 restrains, soybean nucleic acid 8.15 restrains, other nutritional labelings 4.16 restrain.
Claims (1)
1. the composition of gangliosides and bean food is characterized in that said composition comprises gangliosides and bean food;
Wherein: the weight percent content of gangliosides counts 2～10% with the nutrition composition in the bean food, and the bean food nutrition composition is 90～98%;
Said bean food is selected from soya-bean milk, soymilk, instant bean or soyabean protein powder;
Said bean food nutrition composition comprises soybean protein, soybean lecithin, soyasaponins, isoflavones, soybean nucleic acid, soyabean oligosaccharides, each biostearin, mineral matter or phytosterol.
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| CN103141841B (en) * | 2013-03-14 | 2014-10-15 | 吉林省中韩动物科学研究院 | Method for preparing memory improvement product with cartialgenous |
| CN103251651B (en) * | 2013-05-20 | 2015-03-18 | 吉林省中韩动物科学研究院 | Extracting method of animal ganglioside and cerebroside |
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| EP2211629B1 (en) | 2007-10-19 | 2020-07-08 | Fonterra Co-Operative Group Limited | Methods of maintaining or increasing growth or cognitive development |
| EP3763219A1 (en) * | 2007-10-19 | 2021-01-13 | Fonterra Co-Operative Group Limited | Methods of maintaining or increasing growth or cognitive development |
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