CN1259409C - A magnetic bioreactor - Google Patents

Abstract

The magnetic bioreactor involved in the present invention includes: a reaction chamber, a magnetic particle inlet pipe, a biological liquid inlet pipe and a biological reaction liquid discharge pipe are installed at the bottom of the reaction chamber, and a circulating material outlet pipe is installed at the upper end; vertically installed at the center of the reaction chamber The central axis of the center shaft is fixed with multi-layer wire mesh medium plates distributed at intervals horizontally; a steel plate is fixed on the upper end of the central axis, a permanent magnet is arranged above the steel plate, and an eccentric wheel driven by a motor is installed near the upper surface of the permanent magnet. The motor drives the eccentric wheel to rotate, and then drives the permanent magnet to reciprocate up and down between the contact with the steel plate and the separation phase; its reciprocating vibration transmits the magnetism to the wire mesh medium plate, and the wire mesh medium plate obtains intermittent magnetism, and the magnetic particles are continuously adsorbed on the wire mesh On the medium, and continuously dispersed and suspended in the reaction chamber, the target biomolecules can have a larger contact surface area and a longer residence time with the magnetic particles, which improves the adsorption efficiency of the magnetic particles, and has a simple structure and is easy to operate.

Description

A magnetic bioreactor

technical field

The invention relates to a bioreactor, in particular to a magnetic bioreactor under a permanent magnetic field.

Background technique

In recent years, magnetic separation technology has been widely used in chemical industry, metallurgy, environmental protection, bioengineering and biomedical engineering and other fields. Especially for the separation of magnetically labeled biomolecules or magnetic particles loaded with biomolecules from biological products, magnetic separation technology shows unique advantages and is the development direction of biochemical separation.

The magnetic reactor is the core part of the magnetic separation process, how to capture the fine magnetic particles and how to improve the adsorption rate of the magnetic particles to the target biomolecules is the key. Increasing the background magnetic field can improve the ability to capture fine magnetic particles. The background magnetic field in the current magnetic separation device gradually develops from an electromagnetic field to a permanent magnetic field. Regarding the magnetic reactor and the magnetic separation device (see Journal of Chromatography A, Vol.690: 1-8, 1995), the structure of the magnetically stabilized bed reaction device is as follows: it includes a reaction vessel located in the electromagnetic field, and the reaction vessel is set Multi-layer adsorption bed, when the magnetic particles undergo adsorption reaction in the adsorption bed layer, due to the small contact area between the target biomolecules and the magnetic particles and the short residence time, the adsorption reaction is quite weak, and many target biomolecules have not yet had time to adsorb with the magnetic particles. The reaction has already entered the next step of operation, which affects the utilization rate and adsorption efficiency of magnetic particles. At the same time, the reactor uses an electromagnet as the background magnetic field, and it is difficult to generate a high magnetic field strength in the bed due to heating and other reasons, resulting in a decrease in adsorption efficiency. Therefore, how to improve the contact area and residence time of target biomolecules and magnetic particles is a key problem to be solved.

Contents of the invention

The object of the present invention is to provide a magnetic bioreactor with a simple structure and under permanent magnetic field, aiming at many defects existing in the existing magnetic separation reactor.

Technical scheme of the present invention is as follows:

The magnetic bioreactor provided by the invention comprises:

A reaction chamber, the bottom of the reaction chamber is equipped with a magnetic particle inlet pipe, a biological liquid inlet pipe and a biological reaction liquid discharge pipe, and a circulating material outlet pipe is installed on the upper end of the reaction chamber;

a central shaft mounted vertically at the center of the reaction chamber; and,

The multi-layer wire mesh medium boards are horizontally fixed and installed at intervals on the central axis;

A steel plate is fixed on the upper end of the central shaft, a permanent magnet is set above the steel plate, and an eccentric wheel driven by a motor to rotate is set near the upper surface of the permanent magnet. The motor drives the eccentric wheel to rotate, and then drives the permanent magnet to contact and separate from the steel plate Up and down reciprocating vibration between phases;

The magnetic field of the permanent magnet is 600-4600 Oersted;

The waveform of the up and down reciprocating vibration of the permanent magnet is a sine wave or other waveforms;

The wire mesh medium plate is a pure wire mesh medium plate with an aperture of 50 μm-100 μm;

The central axis is a pure iron axis; the cross-sectional area of the steel plate is approximately equal to the cross-sectional area of the permanent magnet.

The magnetic bioreactor of the present invention uses a permanent magnetic field instead of an electromagnetic field, and the permanent magnet is driven by an eccentric wheel to reciprocate up and down, and the amplitude and frequency of the vibration can be adjusted. When the permanent magnet vibrates downward, the permanent magnet is in contact with the steel plate connected to the central axis, and the magnetism is transmitted to the central axis, and then to the mesh medium plate, and the magnetic particles will be immediately adsorbed to the mesh medium plate; when the permanent magnet is upward When moving away from the steel plate connected by the central axis, the magnetism of the wire mesh medium plate disappears immediately, and the magnetic particles leave the wire mesh medium plate and disperse and suspend in the reaction chamber. The up and down reciprocating motion of the permanent magnet makes the magnetic particles continuously gather, disperse and suspend in the reaction chamber. In this way, the magnetic particles can have a larger surface area contact with the target biomolecules, have a longer residence time, and greatly increase the magnetic adsorption rate. However, in the previous magnetically stabilized bed reactor, the magnetic particles are fixed and gathered in the reaction chamber under the action of an external magnetic field. Due to long-term magnetization, the particles will agglomerate, resulting in the reduction of the contact area between the target biomolecules and the magnetic particles, and the residence time shortened, the adsorption rate of magnetic particles is low. The invention adopts the up and down reciprocating motion of the permanent magnet to endow the wire mesh medium plate with discontinuous magnetism, so that the magnetic particles can be suspended in the reaction chamber and have a larger contact area and longer contact time with the target biomolecules, which solves the problems in the prior art. The problem of agglomeration of magnetic particles in the magnetically stabilized bed reactor improves the adsorption rate of magnetic particles.

The present invention has the following characteristics:

(1) Use permanent magnets, which have a high background magnetic field.

(2) The permanent magnetic field transmits the magnetism to the wire mesh medium plate through the central axis, utilizing the principle of magnetic transmission.

(3) The up and down reciprocating vibration of the permanent magnetic field makes the wire mesh dielectric plate have intermittent magnetism.

(4) The magnetic particles can be dispersed and suspended in the reaction chamber for a long time.

(5) Magnetic particles have a high adsorption rate for target biomolecules.

(6) Adjusting the vibration frequency of the permanent magnet The wire mesh dielectric plate can obtain magnetism of different frequencies.

(7) The structure of the reactor is simple and the operation is convenient.

Description of drawings:

Fig. 1 is a structural representation of the present invention;

Figure 2 is a schematic diagram of the working state of the magnetic particles when the permanent magnet is in contact with the steel plate;

Fig. 3 is a schematic diagram of the working state of the magnetic particles when the permanent magnet leaves the steel plate;

Among them: reaction chamber 1 permanent magnet 2 steel plate 3

     Central axis 4                                                                                                                      ,

Biological liquid inlet pipe 7 Biological reaction liquid discharge pipe 8 Circulating material outlet pipe 9

Detailed ways

Further describe the present invention below in conjunction with accompanying drawing and embodiment:

Example 1

As shown in Figure 1, the magnetic bioreactor under the permanent magnetic field of the present invention is connected with the central axis 4 by a plurality of wire mesh medium plates 5 in the reaction chamber 1, and a steel plate 3 is connected to the upper end of the central axis, directly above the steel plate 3 Install a permanent magnet 2 and keep a certain distance from the steel plate 3. The permanent magnet 2 is driven by an eccentric wheel to make the permanent magnet 2 reciprocate up and down.

Example 2

Below in conjunction with Fig. 2, Fig. 3 specifically illustrate the working principle of the present invention: the eccentric wheel (not shown in the figure) drives the permanent magnet 2 to continuously reciprocate up and down, and when the permanent magnet 2 vibrates downward, the permanent magnet 2 is in phase with the steel plate 3 contact, the magnetism is transferred to the central axis 4, and then the magnetism is passed to the silk mesh medium plate 5, and the magnetic particles with nucleophilic ligands on the surface in the reaction chamber 1 are immediately adsorbed on the silk mesh medium plate 5; When the magnet 2 moves upwards away from the steel plate 3 connected to the central axis 4, the magnetism of the wire mesh dielectric plate 5 disappears immediately, and the magnetic particles leave the wire mesh dielectric plate 5 and are dispersed and suspended in the reaction chamber 1. In this way, the target biomolecules and the magnetic particles With a larger surface area in contact, the amount of target biomolecules adsorbed by the magnetic particles will be greatly increased. Since the permanent magnet 2 continuously vibrates up and down, the wire mesh dielectric plate 5 also obtains discontinuous magnetism correspondingly, so that the magnetic particles are continuously adsorbed on the wire mesh dielectric plate 5 and are continuously dispersed and suspended in the reaction chamber. Such a cyclical operation allows the target biomolecules to have a larger contact surface area and a longer residence time with the magnetic particles, which improves the adsorption efficiency of the magnetic particles.

Claims (5)

1. A magnetic bioreactor comprising: A reaction chamber, the bottom of the reaction chamber is equipped with a magnetic particle inlet pipe, a biological liquid inlet pipe and a biological reaction liquid discharge pipe, and a circulating material outlet pipe is installed on the upper end of the reaction chamber; a central shaft mounted vertically at the center of the reaction chamber; and The multi-layer wire mesh medium boards are horizontally fixed and installed at intervals on the central axis; A steel plate is fixed on the upper end of the central shaft, a permanent magnet is set above the steel plate, and an eccentric wheel driven by a motor to rotate is set near the upper surface of the permanent magnet. The motor drives the eccentric wheel to rotate, and then drives the permanent magnet to contact and separate from the steel plate Alternate up and down reciprocating vibration. 2. The magnetic bioreactor according to claim 1, wherein the magnetic field of the permanent magnet is 600-4600 Oersted. 3. The magnetic bioreactor according to claim 1, wherein the waveform of the up and down reciprocating vibration of the permanent magnet is a sine wave. 4. The magnetic bioreactor according to claim 1, wherein the wire mesh medium plate is a pure wire mesh medium plate with a pore size of 50 μm-100 μm. 5. The magnetic bioreactor according to claim 1, wherein said central axis is a pure iron axis.

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