CN1257670C - Sterilization composition and application thereof - Google Patents

Abstract

The present invention relates to a germicide composition which comprises two active components of, namely N-methyl-2-[((((1-methyl-3-(2' 6'-dichlorophenyl)-2-propenyl) amino) oxyl) methyl) phenyl]-2-methoxy imino acetamide and a thiocarbamate germicide (selected from mancozeb, mancozeb, zineb, propineb, ambam, mancosult, thiram, ammonium dimethyldithiocarbamate or zinc dimethyldithiocarbamate), a proper amount of surface active agents adacceptable in agriculture and a carrier, wherein the ratio of weight portions of the two active components is between 1 to 100 and 100 to 1. The composition can be respectively prepared into wettable powder, suspendsion agent, aqueous emulsion or water particulate agent, and the germicide composition has obvious synergistic effect. The present invention provides an ideal germicide used for controlling diseases caused by pathogenic bacteria such as oomycetes, phycomycetes, ascomycetes, deuteromycetes, etc.

Description

A kind of bactericidal composition and its application

technical field

The invention belongs to the field of agricultural fungicides and relates to the preparation and application of a fungicide composition containing two active components.

Background technique

N-methyl-2-[2-((((1-methyl-3-(2',6'-dichlorophenyl)-2-propenyl)amino)oxy)methyl)phenyl] -2-Methoxyiminoacetamide is a Strobilurin fungicide (test code SYP-1620) independently created by Shenyang Research Institute of Chemical Industry, which can effectively control fungicides caused by Oomycetes, Phycomycetes, Ascomycetes and Semimycetes. It has excellent control effect on various plant diseases. The bactericide has low dosage, low toxicity, no carcinogenic and mutagenic effects, and can be quickly degraded in plants, soil and water, and will not cause adverse effects on the environment. See Chinese patent application CN1309897A for its preparation example and application. At present, there is no report about the bactericidal composition of this compound and other specific compounds at home and abroad.

Contents of the invention

To avoid N-methyl-2-[2-((((1-methyl-3-(2',6'-dichlorophenyl)-2-propenyl)amino)oxy)methyl)benzene Base]-2-methoxyiminoacetamide single agent may cause problems such as the occurrence of resistance and the decline in drug efficacy. The invention discloses a fungicide containing the fungicide and thiocarbamate fungicides The bactericidal composition, preparation and application of the composition.

The bactericidal composition proposed by the present invention contains two active components A and B, and an appropriate amount of agriculturally acceptable surfactant and carrier.

Active ingredient A is selected from N-methyl-2-[2-((((1-methyl-3-(2',6'-dichlorophenyl)-2-propenyl)amino)oxy) Methyl) phenyl] -2-methoxyiminoacetamide, structural formula is as follows:

Active component B is selected from any of the following thiocarbamate fungicides:

1. Multi-component coordination compound of manganese ethylene bisdithiocarbamate and zinc salt (mancozeb, mancozeb);

2. Manganese ethylene bisdithiocarbamate (maneb, maneb);

3. Ethylene bis(zinc dithiocarbamate) (zineb, Zineb);

4. Zinc propenyl bisdithiocarbamate (propineb, propineb);

5. Ammonium ethylene bisdithiocarbamate (amobam, ammonium dysonium);

6. Ethylene bis (dithiocarbamate) metal coordination compound (cufraneb, Dyson salt);

7. Tetramethylthiuram disulfide (thirm, thiram);

8. N, N dimethylammonium dithiocarbamate (diram, thiramium);

9. Bis(dimethyldithiocarbamate) zinc (ziram, ziram).

More preferred active ingredient B is mancozeb, propineb, mancozeb or thiram.

The ratio by weight of the active components A and B is 1:100 to 100:1, preferably 1:50 to 50:1, more preferably 1:20 to 20:1.

The content of the active components in the composition of the present invention depends on the application amount when used alone, also depends on the mixing ratio of one compound and another compound and the degree of synergistic effect, and is also related to the target fungus. Usually the weight percentage of the active ingredient in the composition is 0.5-90% of the total weight, preferably 5% to 85%. Depending on the type of preparation, the content range of the active ingredient is different. Generally, liquid preparations contain 1-70% by weight active substance, preferably 5-40%; solid preparations contain 5% to 85% by weight active substance, preferably 10-40%; suspension The formulations contain 10-60% by weight of active substance, preferably 5-35%.

The fungicidal composition of the present invention contains at least one surfactant to facilitate the dispersion of the active ingredient in water during application and to facilitate its correct application to plants. The surfactant content is 5-30% of the total weight of the preparation, and the balance is solid or liquid diluent. The surfactant selected for the bactericidal composition of the present invention is well known to those skilled in the art: it can be selected from one or more of emulsifiers, dispersants, wetting agents, adhesives or defoamers. Wherein the emulsifier can be anionic and/or nonionic emulsifier, such as alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, benzylphenol polyoxyethylene ether, polyoxyethylene fatty acid ester, Polyoxyethylene fatty alcohol ether, polyoxyethylene fatty ammonia, etc. Specific examples such as Nongru 500′, Nongru 600 ^# , Nongru 1601, Nongru OP-10, Nongru MOA-3, Nongru OX-2681, Nongru OX-8686, Nongru OX-2501, Nongru OX -8503, NONGRI OX-0507, NONGRIU OX-810, NONGRIU OX-830, NONGRIU 0X-635, NONGRIU 2201, NONGRIU 0201B, NONGRIU 0203B, etc. The dispersant can be alkylnaphthalene sulfonate, bis(alkyl) naphthalene sulfonate formaldehyde condensate, naphthalenesulfonic acid formaldehyde condensate, alkylphenol polyoxyethylene phosphate, alkylphenol polyoxyethylene ether formaldehyde Condensate sulfate, aralkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether, castor oil ethylene oxide adduct, ethylene oxide-propylene oxide block copolymer alkylphenol poly Oxyethylene ether formaldehyde condensate, alkylphenol polyoxyethylene polyoxypropylene ether and methyl cellulose, etc. Specific examples such as sodium lignosulfonate (calcium), methyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate formaldehyde condensate, sodium methylene naphthalene sulfonate, sodium oleic acid methyl amino ethyl sulfonate, epoxy poly Ether, p-tert-butyl ether, dibutylnaphthalenesulfonic acid formaldehyde condensate, etc. The wetting agent can be: sodium lauryl sulfate, sodium alkyl alcohol polyoxyethylene ether sulfate, octylphenol polyoxyethylene ether sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylsulfonate Sodium Naphthalene Sulfonate, Sodium Alkyl Naphthalene Sulfonate, Sorbitan Fatty Acid Ester Polyoxyethylene Ether, etc. Binders can be synthetic or natural, such as xanthan gum, polyvinylpyrrolidone, magnesium aluminum silicate, polyvinyl alcohol, polyethylene glycol, phenolic resins, shellac, carboxymethylcellulose, and sodium alginate, among others . The defoaming agent can be foam enemy, silicone, C _{8 ~ 10} fatty alcohol, C _{10 ~ 20} saturated fatty acid (such as capric acid) and amides, etc. According to different dosage forms, the preparation may also contain disintegrants, wetting and spreading agents, suspending agents and the like known to those skilled in the art.

The fungicidal composition of the present invention contains at least one appropriate amount of solid or liquid diluent according to the different dosage forms prepared. Generally, liquid diluents suitable for preparing the germicidal formulations of the present invention are selected from organic solvents, including alkylbenzenes, alkylnaphthalenes, chlorinated aromatic hydrocarbons, aliphatic hydrocarbons, chlorinated aliphatic hydrocarbons. Particularly suitable, however, are polar solvents, such as alcohols, esters, ketones, but also dimethylformamide, dimethylsulfoxide and N-methyl-pyrrolidone; also vegetable oils and methyl cellosolve. Mixtures of different liquids are often suitable. Organic solvents can also be used as cosolvents or antifreezes. In general, any agriculturally acceptable solid diluent used in formulating fungicide formulations can be used. They can be combined with the active ingredient to facilitate the application of the active ingredient to a target acceptable to the treated plant. Commonly used solid diluents such as diatomaceous earth, magnesium aluminum silicate, activated clay, kaolin, white carbon black, light calcium carbonate, ammonium sulfate, hexamethylenediamine, solid fertilizers, clay, montmorillonite, gypsum, bentonite and Soluble starch, etc.

The compositions of the present invention may be diluted before use or used directly by the user. Its preparation can be prepared by the usual processing method, that is, after the active substance is mixed with a liquid solvent and/or a solid carrier, and then added into a surfactant such as an emulsifier, dispersant, stabilizer, wetting agent, binder, defoamer, etc. one or more of.

The fungicidal composition of the present invention can be processed into any agriculturally acceptable dosage form as required. Examples include suspensions, aqueous emulsions, wettable powders, suspensions, and (water-dispersible) granules. Among them, the preferred dosage forms are suspension concentrates, wettable powders, and (water dispersible) granules. The preparation methods of several dosage forms of the bactericidal composition of the present invention are exemplified as follows:

Suspension preparation: The content of active ingredients in common formulations is 5% to 35%. Using water as the medium, add the original drug, dispersant, suspending agent and antifreeze into a sand mill for grinding to make a suspension.

Preparation of emulsion in water: Add the original drug, solvent and emulsifier together to dissolve into a uniform oil phase. Mix water, antifreeze, etc. together to form a homogeneous water phase. Under high-speed stirring, add the water phase to the oil phase or add the oil phase to the water phase to form a water emulsion with good dispersibility. The active component content of the aqueous emulsion of the present invention is generally 5%-15%. To prepare a concentrated emulsion, the compound of the present invention can be dissolved in one or several mixed solvents, and then an emulsifier is added to enhance the dispersion effect of the compound in water.

Preparation of wettable powder: According to the formula requirements, the original drug, various surfactants and solid diluents are fully mixed, and after being pulverized by an ultra-fine pulverizer, a wettability powder with a predetermined content (such as 10%-40%) can be obtained. Powder products. To prepare wettable powders suitable for spraying, the compounds according to the invention can be mixed with finely divided solid powders such as clays, inorganic silicates, carbonates and wetting, binding and/or dispersing agents.

Preparation of water-dispersible granules: mix and pulverize the original drug, powdery solid diluent, wetting and spreading agent, and binder, add water and knead, then add to a granulator equipped with a certain size screen. Granulate, then dry and sieve (according to the size of the sieve). The original drug, dispersant, disintegrating agent, wetting agent and solid diluent can also be added to a sand mill, ground with water as a medium to make a suspension, and then spray-dried and granulated, usually with a preparation content of 20% ~30% granular product.

The compositions according to the invention may also contain other ingredients, for example other compounds having herbicidal, insecticidal or fungicidal properties, especially protective fungicides. It can also be mixed with nematicides, protective agents, growth regulators, plant nutrients or soil conditioners, whereby additional advantages and effects can be produced.

Of particular interest to the present invention is increasing the duration of the protective activity of the compounds of the invention. Using a carrier that migrates to the plant to protect and slowly releases the fungicidal compound, this slow release formulation can be placed in the soil near the roots of the vine plant; or contain an adhesive component so that it can be applied directly on the On the stem of a vine.

The compositions of the present invention can be applied by conventional methods, such as pouring, spraying, spraying, dusting, spreading or fuming. The application rates of the present invention vary with weather conditions or crop conditions. The time of administration can be before or after infection. This is important because the actual infection is not immediately recognizable. The duration of protection is usually related to the individual compound chosen, but also to many external factors, such as climate, whose effects can often be mitigated by using appropriate formulations.

The fungicide composition of the present invention has both protective, curative and eradicating activities, and can be used for many kinds of crops such as cucumber, wheat, tomato, corn, peanut, rice, grape, pepper, bell pepper, eggplant, tomato, lettuce, cabbage, strawberry , zucchini, and flowers, Chinese herbal medicines and lawns, control plant diseases caused by pathogens of Oomycetes, Phycomycetes, Ascomycetes and Deuteromycetes, such as powdery mildew, downy mildew, early blight, late blight, black star disease, rust, glume blight, verticillium wilt, leaf mold, brown spot, anthracnose, net spot, leaf spot, blight, rice blast, gray mold, frost blight, rot, sudden Inverted disease, black stem disease, canker disease, white rust, (millet) white disease, etc.

The present invention also proposes the use of the above-mentioned fungicidal composition and a method for catching and killing fungi on the spot, comprising treating the site with the composition, which can be, for example, a plant attacked by the fungus, a seed of the plant or a medium in which the plant grows .

Therefore, the composition of the present invention has the following three advantages: one is that the composition shows a significant synergistic effect within a certain proportion range, which not only improves the disease prevention effect, reduces the use cost per mu, but also expands the bactericidal spectrum . Second, because the mechanism of action of each single agent in the composition is different, there is no problem of cross-resistance, so the application of the composition can delay the occurrence and development of drug resistance of pathogens, and help prolong the use of the agent Effect, the purpose of reducing the probability of drug resistance. The third is to improve safety, and the impact of mixing on the environment will be lower and safer.

Detailed ways

In order to make the advantages of the present invention more obvious, the following specific examples are used to describe in detail, but the present invention is by no means limited to these examples. The compound SYP-1620 in the formula is the active ingredient A: N-methyl-2-[2-((((1-methyl-3-(2',6'-dichlorophenyl)-2-propene Base) amino) oxy) methyl) phenyl] -2-methoxyiminoacetamide; the addition amount of each component is weight percentage.

Embodiment 1 (48% wettable powder)

Compound SYP-1620 8%

Mancozeb 40%

Octylphenol polyoxyethylene ether sulfate 2%

Sodium tripolyphosphate 5%

Arylphenol polyoxyethylene ether succinate sulfonate 5%

Calcium Carbonate Light Supplement to 100%

Embodiment 2 (40% wettable powder)

Compound SYP-1620 5%

Propineb 35%

Alkyl succinate sulfonate 2%

Calcium Lignosulfonate 5%

Alkylphenol polyoxyethylene ether formaldehyde condensate sulfonate 5%

Calcium Carbonate Light Supplement to 100%

Embodiment 3 (35% suspending agent)

Compound SYP-1620 7%

Dysenium 28%

Sodium naphthalenesulfonate formaldehyde condensate 5%

White carbon black 0.3%

Xanthan Gum 0.1%

Ethylene Glycol 4%

Bubble Enemy A Little

Water make up to 100%

Embodiment 4 (40% suspending agent)

Compound SYP-1620 5%

Mancozeb 35%

Sodium methylnaphthalenesulfonate formaldehyde condensate 5%

Bentonite 0.8%

Farm Milk 203B 1.5%

Glycerol 4%

Bubble Enemy A Little

Water make up to 100%

Embodiment 5 (60% water dispersible granule)

SYP-1620 10%

Zinc 50%

Sodium methylnaphthalenesulfonate formaldehyde condensate 12%

Sodium N-methyl-oleoyl-taurate 8%

Polyvinylpyrrolidone 2%

Corn starch 2%

Kaolin to make up to 100%

Embodiment 6 (20% wettable powder)

Compound SYP-1620 19%

Maneb 1%

Sodium Lauryl Sulfate 1.5%

p-tert-butyl ether 2%

Sodium lignosulfonate 8%

Calcium Carbonate Light Supplement to 100%

Embodiment 7 (42% water dispersible granule)

Compound SYP-1620 2%

Firam 40%

Sodium methylnaphthalenesulfonate formaldehyde condensate 12%

Sodium N-methyl-oleoyl-taurate 8%

Polyvinylpyrrolidone 2%

Corn starch 2%

Kaolin to make up to 100%

Embodiment 8 (32% suspending agent)

Compound SYP-1620 4%

Firam 28%

Calcium Lignosulfonate 5%

White carbon black 0.3%

Ethylene Glycol 4%

Bubble Enemy A Little

Water make up to 100%

Embodiment 9 (42% wettable powder)

Compound SYP-1620 40%

Maneb 2%

Alkylamido taurate 2%

Alkylnaphthalene formaldehyde condensate 5%

Alkylphenol polyoxyethylene phosphate 5%

Calcium Carbonate Light Supplement to 100%

Embodiment 10 (10% water emulsion)

Compound SYP-1620 5%

Propineb 5%

Polyoxyethylene (n ₂₀ ) phenylethyl

Phenyl ether oleate 8%

Calcium dodecylbenzenesulfonate 16%

Piperonyl Butoxide 15%

Cyclohexanone 10%

Sorbitol 5%

Water make up to 100%

Embodiment 11 (40% water dispersible granule)

Compound SYP-1620 10%

Firam 30%

Sodium methylnaphthalenesulfonate formaldehyde condensate 15%

Sodium Alginate 5%

Macrogol 8000 5%

Sodium Sulfate 15%

Bentonite to make up to 100%

Embodiment 12 (60% water dispersible granule)

Compound SYP-1620 6%

Dysenium 54%

Sodium Lignosulfonate 8.5%

Sodium methylnaphthalenesulfonate formaldehyde condensate 4.6%

Soluble Starch 15%

Gypsum Make up to 100%

Embodiment 13 (36% wettable powder)

Compound SYP-1620 6%

Zinc 30%

Sodium Lauryl Sulfate 1.5%

White carbon black 6%

Calcium Lignosulfonate 2%

Sodium methylnaphthalenesulfonate formaldehyde condensate 8%

Calcium Carbonate Light Supplement to 100%

Embodiment 14 (20% suspending agent)

Compound SYP-1620 4%

Dysenium 16%

Sodium Lignosulfonate 5.8%

Agricultural Milk 0201B 8.5%

OP-10 0.6%

T-20 0.5

Ethylene Glycol 4%

Water make up to 100%

Embodiment 15 (16% water emulsion)

Compound SYP-1620 2%

Mancozeb 14%

Tristyrene phenol + ethylene oxide,

Phosphorylated, triethanolamine neutralized 8.5%

Alkyl-Diethylene Glycol Ether-Sodium Sulfonate 2.5%

Farm Milk 2201 13%

Dimethylformamide 8%

Ethylene Glycol 5%

Water make up to 100%

Embodiment 16 (24% wettable powder)

Compound SYP-1620 4%

Zinc 20%

Sodium Lauryl Sulfate 1.5%

p-tert-butyl ether 2%

Sodium Lignosulfonate 8%

White carbon black 5%

Calcium Carbonate Light Supplement to 100%

Embodiment 17 (60% water dispersible granule)

Compound SYP-1620 30%

Propineb 30%

Sodium Methylene Naphthalene Sulfonate 5%

Epoxy polyether 5%

Sodium Sulfate 15%

Gypsum Make up to 100%

Embodiment 18 (10% wettable powder)

Compound SYP-1620 5%

Zinc 5%

Sodium Lauryl Sulfate 1.5%

Carboxymethylcellulose 1%

Sodium Lignosulfonate 2%

Sodium methylnaphthalenesulfonate formaldehyde condensate 2.5%

White carbon black 0.2%

Calcium Carbonate Light Supplement to 100%

Embodiment 19 (40% wettable powder)

Compound SYP-1620 5%

Propineb 30%

Sodium dodecylbenzenesulfonate 1%

White carbon black 10%

Naphtholsulfonic acid formaldehyde condensate sodium salt 10%

Calcium Carbonate Light Supplement to 100%

bioassay test

1 in vitro activity test

The in vitro biological activity measurement adopts the method of toxic medium, that is, adding quantitative test agents (the test dose is calculated according to the active ingredient) in the PDA medium, inoculating the test pathogenic bacteria after cooling, and culturing in the dark at 25 °C. When the diameter of the blank control colony grows to 40-50 mm, measure the growth diameter of the colony of different treatments, and calculate the bacteriostatic rate and EC ₅₀ value of each treatment. The test results are shown in Table 1-Table 3.

Table 1 Tomato early blight (Alternaria solani) treatment agent Bacteriostatic rate (%) of each treatment dose _EC50 1.6μg/ml 0.8μg/ml 0.4μg/ml 0.2μg/ml 0.1μg/ml Example 1 73.54% 66.78% 49.35% 41.67% 32.01% 0.33 5% SYP-1620 EC 60.20% 48.87% 42.15% 34.33% 21.28% 0.77 70% mancozeb wettable powder 65.84% 52.17% 45.90% 36.78% 27.21% 0.57

Table 2 Corn spot disease (Bipolaris mayghi) treatment agent Bacteriostatic rate (%) of each treatment dose _EC50 Example 2 1μg/ml 0.5μg/ml 0.25μg/ml 0.13μg/ml 0.06μg/ml 0.09 96.14% 80.78% 72.35% 69.67% 33.64% 5% SYP-1620 EC 1μg/ml 0.5μg/ml 0.25μg/ml 0.13μg/ml 0.06μg/ml 0.10 95.45% 83.75% 70.41% 62.11% 35.89% 70% Propineb WP 2μg/ml 1μg/ml 0.5μg/ml 0.25μg/ml 0.13μg/ml 0.62 80.24% 55.12% 43.67% 30.48% 20.07%

Table 3 Verticillium dahliae on eggplant treatment agent Bacteriostatic rate (%) of each treatment dose _EC50 2μg/ml 1μg/ml 0.5μg/ml 0.25μg/ml 0.13μg/ml Example 3 88.89% 76.15% 54.18% 44.24% 33.90% 0.32 5% SYP-1620 EC 80.67% 70.21% 56.18% 50.01% 40.42% 0.27 40% Disenium water solution 70.42% 58.67% 49.26% 38.75% 30.14% 0.52

2 Bioactivity test of greenhouse potted plants

In the indoor test, the seedling pot method was adopted, and the test crops were potted in the greenhouse until the 2-3 leaf stage. Foliar spray treatment was carried out on the crop sprayer according to the designed dosage, and the test pathogenic bacteria were inoculated after 24 hours of chemical treatment. After the inoculation, the test materials were all kept moist and cultivated in the artificial climate chamber. Disease grading and control efficacy calculation methods refer to the National Standard of the People's Republic of China "Guidelines for Field Efficacy Tests of Pesticides". The test shows that the bactericidal composition of the present invention has a very excellent control effect on the tested target, and the pot plant biological activity of the mixed agent is obviously higher than that of the single agent, and both have obvious synergistic effects. The test results are shown in Table 4 to Table 8:

Table 4 Cucumber powdery mildew (Erysiphe cucurbitacearum) treatment agent Treatment dose and control effect Example 4 100μg/ml 50μg/ml 25μg/ml 100.00% 95.27% 88.75% 5% SYP-1620 EC 100μg/ml 50μg/ml 25μg/ml 100.00% 100.00% 92.50% 70% mancozeb wettable powder 800μg/ml 400μg/ml 200μg/ml 89.75% 69.24% 30.65%

Table 5 Cucumber downy mildew (Psudoperonospora cubensis) treatment agent Treatment dose and control effect Example 5 100μg/ml 50μg/ml 25μg/ml 97.22% 77.78% 66.67% 5% SYP-1620 EC 100μg/ml 50μg/ml 25μg/ml 97.22% 88.89% 77.78% 50% Zinc wettable powder 800μg/ml 400μg/ml 200μg/ml 77.78% 61.11% 33.33%

Table 6 Tomato late blight (Phytophthora infestans) treatment agent Treatment dose and control effect Example 6 100μg/ml 50μg/ml 25μg/ml 100% 92.67% 87.44% 5% SYP-1620 EC 100μg/ml 50μg/ml 25μg/ml 100% 93.45% 85.40% 50% maneb wettable powder 800μg/ml 400μg/ml 200μg/ml 69.81% 57.24% 40.13%

Table 7 Peanut brown spot (Cercospora arachidecola) treatment agent Treatment dose and control effect Example 7 800μg/ml 400μg/ml 200μg/ml 62.78% 64.27% 27.19% 5% SYP-1620 EC 200μg/ml 100μg/ml 50μg/ml 77.55% 70.23% 64.37% 50% thiram wettable powder 800μg/ml 400μg/ml 200μg/ml 65.28% 60.21% 25.20%

Table 8 Wheat powdery mildew (Blumeria grmainis) treatment agent Treatment dose and control effect Example 8 100μg/ml 50μg/ml 25μg/ml 100.00% 98.67% 80.46% 5% SYP-1620 EC 100μg/ml 50μg/ml 25μg/ml 100.00% 100.00% 94.65% 50% thiram wettable powder 800μg/ml 400μg/ml 200μg/ml 78.45% 66.34% 27.81%

3 Control effect of field plots

The experiment was carried out under field conditions, and the cultivation and management of the experimental crops were carried out according to conventional agricultural production. In the field drug efficacy determination, 3 doses of the test agents were set, and a blank control without application of the drug was set, and 4 repetitions were made. In the experiment, pesticides were applied in the field without disease or at the early stage of disease, and the pesticide application equipment was a knapsack manual sprayer. Natural onset, the result investigation was carried out when the blank control without drug application fully developed the onset. Disease grading and control efficacy calculation methods are in accordance with the national standard of the People's Republic of China, "Guidelines for Field Efficacy Tests of Pesticides". Test results The fungicidal composition of enoxastrobin and thiocarbamates has a very excellent control effect on disease control, and the test results are shown in Tables 9 to 12.

Table 9 Pear scab (Fusicladium virescens) treatment agent Treatment dose and control effect Example 1 400g.ai/ha 200g.ai/ha 100g.ai/ha 100.00% 98.07% 80.65% 5% SYP-1620 EC 200g.ai/ha 100g.ai/ha 50g.ai/ha 100.00% 97.77% 80.64% 70% mancozeb wettable powder 1200g.ai/ha 800g.ai/ha 400g.ai/ha 65.25% 43.22% 40.56%

Table 10 Tomato leaf mold (Fulvia fulva) treatment agent Treatment dose and control effect Example 10 400g.ai/ha 200g.ai/ha 100g.ai/ha 95.68% 92.55% 84.67% 5% SYP-1620 EC 400g.ai/ha 200g.ai/ha 100g.ai/ha 94.00% 90.00% 81.82% 70% Propineb WP 1200g.ai/ha 800g.ai/ha 400g.ai/ha 85.63% 80.54% 69.45%

Table 11 Grape anthracnose (Glomerella cingulata) treatment agent Treatment dose and control effect Example 11 400g.ai/ha 200g.ai/ha 100g.ai/ha 89.28% 81.56% 69.25% 5% SYP-1620 EC 200g.ai/ha 100g.ai/ha 50g.ai/ha 84.64% 80.20% 62.34% 50% thiram wettable powder 1200g.ai/ha 800g.ai/ha 400g.ai/ha 74.31% 63.15% 42.80%

Table 12 Cucumber powdery mildew (Erysiphe cihporacearum) treatment agent Treatment dose and control effect Example 12 200g.ai/ha 100g.ai/ha 50g.ai/ha 100% 85.85% 71.20% 5% SYP-1620 EC 100g.ai/ha 50g.ai/ha 25g.ai/ha 100% 96.33% 85.00% 70% mancozeb wettable powder 800g.ai/ha 400g.ai/ha 200g.ai/ha 74.51% 56.64% 34.85%

Claims (6)

1, a kind of bactericidal composition, contain two kinds of active components of A, B, it is characterized in that: A is selected from N-methyl-2-[2-((((1-methyl-3-(2 ', 6 '-dichlorophenyl)-and the 2-acrylic) amino) the oxygen base) methyl) phenyl]-2-methoxyl group imido grpup acetamide, B is selected from following any one Carbamates bactericidal agent: mancozeb, maneb, zineb, Propineb, ambam or tmtd; The ratio of weight and number of A, two kinds of active components of B is 1: 20 to 20: 1; Active component weight percentage in the composition is 10-60%.

2, bactericidal composition according to claim 1 is characterized in that: described B component is selected from mancozeb, Propineb, ambam or tmtd.

3, bactericidal composition according to claim 1 is characterized in that: contain at least a carrier and at least a surfactant in the composition.

4, bactericidal composition according to claim 1 is characterized in that: said composition can be mixed with wetting powder, suspending agent, aqueous emulsion or water dispersible granules.

5, bactericidal composition according to claim 1 is used to prevent and treat the purposes by Oomycete, Phycomycetes, Ascomycetes and the microbial disease of deuteromycetes cause of disease.

6, catch and kill the method for fungi at the scene, comprise with the described bactericidal composition of claim 1 and handle this place, the wherein on-the-spot plant of standing fungal attack, the seed of these plants or the medium of plant growing of comprising.