CN1244111C - A kind of preparation method of gold nanoprobe - Google Patents
A kind of preparation method of gold nanoprobe Download PDFInfo
- Publication number
- CN1244111C CN1244111C CN 03128332 CN03128332A CN1244111C CN 1244111 C CN1244111 C CN 1244111C CN 03128332 CN03128332 CN 03128332 CN 03128332 A CN03128332 A CN 03128332A CN 1244111 C CN1244111 C CN 1244111C
- Authority
- CN
- China
- Prior art keywords
- trisodium citrate
- sodium
- gold
- sodium borohydride
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 20
- 239000010931 gold Substances 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title abstract description 13
- 239000002245 particle Substances 0.000 claims abstract description 27
- 239000001509 sodium citrate Substances 0.000 claims abstract description 17
- 239000012279 sodium borohydride Substances 0.000 claims abstract description 14
- 229910000033 sodium borohydride Inorganic materials 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims abstract 4
- 235000019333 sodium laurylsulphate Nutrition 0.000 claims abstract 3
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims description 13
- 229940038773 trisodium citrate Drugs 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims 3
- 239000000523 sample Substances 0.000 abstract description 13
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 abstract 4
- SJUCACGNNJFHLB-UHFFFAOYSA-N O=C1N[ClH](=O)NC2=C1NC(=O)N2 Chemical compound O=C1N[ClH](=O)NC2=C1NC(=O)N2 SJUCACGNNJFHLB-UHFFFAOYSA-N 0.000 abstract 3
- 239000004141 Sodium laurylsulphate Substances 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 12
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 239000002105 nanoparticle Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 239000003638 chemical reducing agent Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 125000000129 anionic group Chemical group 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- -1 mercapto alkane compound Chemical class 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 230000005476 size effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
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- Manufacture Of Metal Powder And Suspensions Thereof (AREA)
- Powder Metallurgy (AREA)
Abstract
The present invention relates to a preparation method for a gold nanon probe. Chlorauric acid and sodium citrate are mixed in water, and then the sodium citrate solution of sodium borohydride is filled in the mixed chlorauric acid and sodium citrate; when the sodium citrate solution of sodium borohydride is filled, sodium lauryl sulphate is filled; thus, a gold nanon probe of which the particle diameter is four to six nanons can be obtained, wherein the filling quantity of the dodecyl sodium sulfate is 0.3 to 3 times more than the quality of the chlorauric acid. The gold nanon particles obtained by the present invention have small particle diameters and good monodispersity and still have good biologic affinity to protein.
Description
Technical field
The present invention relates to a kind of preparation method of gold nano-probe, is exactly that the preparation particle diameter is 4～6 gold nano-probe by the influence of SDS to gold atom depositional mode and gold nano nucleus growth behavior specifically.
Background technology
Quantum size effect that golden nanometer particle has and high-specific surface area cause it to produce performances such as many special light, electricity, catalysis, thereby are widely used in many high-technology fields such as biomarker, DNA sensor, molecular recognition, nano-electrode.Because the golden nanometer particle of small particle diameter has preferably penetrability, has special electrology characteristic in electronics research in cytochemistry, so the technology of the golden nanometer particle that preparation size is little, monodispersity is good seems important day by day.
In the prior art, the preparation particle diameter is during less than the golden nanometer particle of 10nm, usually use in water or oil phase with strong reductant (sodium borohydride, ascorbic acid etc., used sodium borohydride is prepared with citric acid three sodium solution usually) with the chemical reduction method of gold chloride reaction, but along with reducing of nano particle diameter, dispersion degree worse and worse.Along with nano particle diameter reduces, specific surface area increases simultaneously, surfactancy increases, and nano-particle solution stability reduces easily assembles.Although can stop the gathering of particle by coverings such as adding mercapto alkane compound and organic polymers.But the combining relatively firmly of covering and golden nanometer particle (as forming the Au-S key) in these methods divides the period of the day from 11 p.m. to 1 a.m to be restricted at further modified biological.
Summary of the invention
Problem to be solved by this invention is exactly: a kind of method for preparing the small particle diameter gold nano-probe is provided, and the gold nano-probe particle diameter of this method preparation is less and monodispersity good.
Technical scheme provided by the invention is: a kind of method for preparing gold nano-probe, gold chloride is mixed in water with trisodium citrate, the citric acid three sodium solution that adds sodium borohydride then, its key is: the citric acid three sodium solution of sodium borohydride is mixed with lauryl sodium sulfate, join in the reaction solution after mixing, and obtain the gold nano-probe that particle diameter is 4～6 nanometers, wherein, the addition of lauryl sodium sulfate is 0.3～3 times of gold chloride quality.
In the above-mentioned reaction solution, gold chloride is 1: 132 with the mol ratio that trisodium citrate mixes in water; The mass ratio that sodium borohydride mixes with trisodium citrate is 3: 40.
The citric acid three sodium solution of used sodium borohydride is the sodium borohydride solution of suggestion with the 0.075wt% of the trisodium citrate preparation of 38.8mmol/L.
The present invention is at NaBH
4Utilize of the electrostatic adsorption of anionic SDS water-wet side in the reducing process to the golden nanometer particle surface, suppressed gold atom sedimentation velocity, limited the growth rate of nucleus, and in the golden nanometer particle propagation process, stoped the generation of new nucleus, improved the monodispersity of small particle diameter golden nanometer particle effectively, and the particle diameter of gained golden nanometer particle is little, is 4～6 nanometers.The golden nanometer particle of the present invention's preparation has biological affinity preferably to protein remains.
Description of drawings
Accompanying drawing is the TEM figure (scale is 50nm) of the gold nano-probe of the present invention's preparation.
Embodiment
The preparation of small particle diameter gold nano-probe: the HAuCl of 50 μ L 1wt%
4Mix with the water of 4.5mL and place round-bottomed flask to stir, add the trisodium citrate of the 38.8mmol/L of 5mL behind the 1min.Stir 1min again, add the 0.075wt%NaBH of 50 μ L with the preparation of 38.8mmol/L trisodium citrate
4(NaBH
4Solution and lauryl sodium sulfate mix quick adding, and the addition of lauryl sodium sulfate is 0.3～3 times of gold chloride quality), continue to stir 5min then, filter and promptly get the gold nano-probe that required particle diameter is 4～6 nanometers.Observed result such as accompanying drawing under transmission electron microscope (TEM), it is good that the present invention makes small particle diameter gold nano-probe monodispersity.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03128332 CN1244111C (en) | 2003-07-17 | 2003-07-17 | A kind of preparation method of gold nanoprobe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03128332 CN1244111C (en) | 2003-07-17 | 2003-07-17 | A kind of preparation method of gold nanoprobe |
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| Publication Number | Publication Date |
|---|---|
| CN1482622A CN1482622A (en) | 2004-03-17 |
| CN1244111C true CN1244111C (en) | 2006-03-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03128332 Expired - Fee Related CN1244111C (en) | 2003-07-17 | 2003-07-17 | A kind of preparation method of gold nanoprobe |
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| CN (1) | CN1244111C (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100600938B1 (en) * | 2004-07-26 | 2006-07-13 | 한국표준과학연구원 | Gold nanostructures and methods of manufacturing the same |
| CN100431754C (en) * | 2007-03-02 | 2008-11-12 | 江南大学 | Process for preparing gold nano particle by using aqueous phase soft mould plate method |
| CN101551385B (en) * | 2007-09-03 | 2014-07-16 | 深圳市人民医院 | Double labelling Nano-Au probe and preparation method |
| CN102221614B (en) * | 2011-03-28 | 2013-07-17 | 山东大学 | Analysis method for identifying effect target of small molecular compound by using gold nanoprobe |
| CN102507858B (en) * | 2011-10-25 | 2014-10-22 | 广东省药品检验所 | Rapid screening method of illegally-recycled waste cooking oil |
| CN102706866B (en) * | 2012-05-18 | 2014-12-03 | 中国科学院宁波材料技术与工程研究所 | Detection reagent for rapidly detecting multiple single metal ions, preparation and application thereof |
| CN105675602B (en) * | 2016-03-10 | 2019-01-04 | 河南科技学院 | A kind of manganese ion detection method and detection probe |
| GB201818923D0 (en) * | 2018-11-21 | 2019-01-02 | Univ Leeds Innovations Ltd | Nanomaterials |
| CN109724971A (en) * | 2019-02-01 | 2019-05-07 | 莱华尔科技有限公司 | A kind of preparation method and application of food sensing solution using gold nanoparticles |
-
2003
- 2003-07-17 CN CN 03128332 patent/CN1244111C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1482622A (en) | 2004-03-17 |
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