CN113049800B

CN113049800B - Immunoassay analyzer, detection method thereof and computer readable storage medium

Info

Publication number: CN113049800B
Application number: CN201911384361.XA
Authority: CN
Inventors: 王锐; 覃桂贵; 陆锋
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2019-12-28
Filing date: 2019-12-28
Publication date: 2024-05-28
Anticipated expiration: 2039-12-28
Also published as: CN113049800A

Abstract

The application discloses an immunoassay analyzer, a detection method thereof and a computer readable storage medium, wherein the detection method of the immunoassay analyzer comprises the following steps: obtaining a sample to be detected; if the sample to be detected is a serum sample or a plasma sample, inputting the sample to be detected into an immunoassay detection channel for detection, and obtaining a first immunoassay result; if the sample to be detected is a whole blood sample, inputting part of the sample to be detected into an immunoassay detection channel for detection to obtain a whole blood detection result, inputting part of the sample to be detected into an HCT calculation channel to obtain a blood cell detection result, and calculating to obtain a second immunoassay result according to the whole blood detection result and the blood cell detection result. By the mode, equipment cost is saved, and detection efficiency is improved.

Description

Immunoassay analyzer, detection method thereof and computer readable storage medium

Technical Field

The application relates to the technical field of immunoassay, in particular to an immunoassay instrument, a detection method thereof and a computer readable storage medium.

Background

The immunoassay detection equipment is subjected to several different development periods such as radioimmunoassay, fluorescent immunoassay, enzyme-labeled immunoassay, chemiluminescent immunoassay and the like, and the full-automatic chemiluminescent immunoassay is a new development stage of the current immunoassay detection, has the characteristics of environment friendliness, rapidness and accuracy, and has been widely accepted by the market.

The chemiluminescent immunoassay analyzer is an in vitro diagnostic instrument for performing an immunoassay on a human body by detecting a serum sample. The common chemiluminescence immunoassay analyzer needs to separate samples before detection, and serum or plasma samples are used as test objects to start detection after separation, so that the types of the samples supported by the detection equipment are single.

Disclosure of Invention

In order to solve the problems, the application provides an immunoassay analyzer, a detection method thereof and a computer readable storage medium, which can save equipment cost and improve detection efficiency.

The application adopts a technical scheme that: there is provided a detection method of an immunoassay analyzer, the method comprising: obtaining a sample to be detected; if the sample to be detected is a serum sample or a plasma sample, inputting the sample to be detected into an immunoassay detection channel for detection, and obtaining a first immunoassay result; if the sample to be detected is a whole blood sample, inputting part of the sample to be detected into an immunoassay detection channel for detection to obtain a whole blood detection result, inputting part of the sample to be detected into an HCT calculation channel to obtain a blood cell detection result, and calculating to obtain a second immunoassay result according to the whole blood detection result and the blood cell detection result.

Before obtaining the sample to be detected, the method further comprises the following steps: judging whether a sample rack is detected; if yes, detecting the type of the sample container on the sample rack; and detecting the type of the sample to be detected in the corresponding sample container according to the type of the sample container.

Wherein detecting the type of sample container on the sample rack comprises: detecting whether a sample tube exists on the sample rack at a first height position by adopting a first sensor; and detecting a height type of the sample tube on the sample rack at a second height position, which is higher than the first height position, using the second sample sensor; detecting the height type of the sample tube on the sample rack at a third height position by using a third sensor, wherein the third height position is higher than the second height position; the type of the sample container on the sample rack is determined according to the detection results of the first sensor, the second sensor and the third sensor.

Wherein the method further comprises: a fourth sensor is combined with the first sensor, the second sensor and the third sensor, and the type of the sample tube on the sample rack is confirmed at a fourth height position, wherein the fourth height position is lower than the first height position; determining the type of the sample container on the sample rack according to the detection results of the first sensor, the second sensor and the third sensor comprises: the type of the sample container on the sample rack is determined according to the detection results of the first sensor, the second sensor, the third sensor and the fourth sensor.

The method for detecting the immune detection of the sample comprises the steps of inputting a sample to be detected into an immune analysis detection channel for detection, and obtaining a first immune detection result, wherein the steps comprise: adding a first reagent into a target reaction cup; adding a sample to be detected into a target reaction cup; sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup; and detecting the liquid in the target reaction cup, and obtaining a first immune detection result.

Wherein, after carrying out the operation of mixing, incubating operation and magnetic separation operation in proper order to the liquid in the target reaction cup, still include: adding a second reagent into the target reaction cup; and (3) sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup for the second time.

Wherein, after evenly mixing operation, incubation operation and magnetic separation operation are carried out in proper order to the liquid in the target reaction cup again, still include: adding a third reagent into the target reaction cup; and (3) sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup for the third time.

Wherein, carry out the operation of mixing, incubation operation and magnetism separation operation to the liquid in the target reaction cup in proper order, include: sequentially and uniformly mixing the liquid in the target reaction cup; placing the target reaction cup in a set temperature environment for incubation operation for a set time length; and performing magnetic separation operation on the liquid in the target reaction cup.

The method for detecting the liquid in the target reaction cup and obtaining the first immune detection result comprises the following steps: uniformly mixing the liquid in the target reaction cup; inputting the liquid in the target reaction cup to an optical detection module so that the optical detection module detects the liquid and a first immune detection result is obtained.

Inputting part of the sample to be detected into the HCT calculation channel, and obtaining a blood cell detection result, wherein the method comprises the following steps: adding a diluent into the impedance detection cell; adding a sample to be detected into the impedance detection pool; uniformly mixing the liquid in the impedance detection pool; and detecting the liquid after the uniform mixing operation in the impedance detection pool, and obtaining a blood cell detection result.

The method for detecting the liquid after the uniform mixing operation in the impedance detection pool and obtaining the blood cell detection result comprises the following steps: detecting the number RBC and average red blood cell volume MCV of a sample to be detected; the hematocrit HCT was calculated as a blood cell test result using the following formula: hct=rbc MCV.

Wherein, calculate and get the second immune detection result according to whole blood detection result and blood cell detection result, including: calculating to obtain a second immune detection result by adopting the following formula; wherein A is the whole blood detection result, and HCT is the blood cell detection result.

The step of inputting part of the sample to be detected into a colorimetric detection channel and obtaining a blood cell detection result comprises the following steps: adding a hemolytic agent into the impedance detection cell; adding the sample to be detected into the impedance detection pool; uniformly mixing the liquid in the impedance detection tank; and detecting the liquid after the uniform mixing operation in the impedance detection pool, and obtaining a blood cell detection result.

The step of detecting the liquid after the uniform mixing operation in the impedance detection pool and obtaining a blood cell detection result comprises the following steps: detecting the hemoglobin quantity HGB and the average hemoglobin concentration MCHC of the sample to be detected; the hematocrit HCT was calculated as a blood cell test result using the following formula:

Wherein the calculating according to the whole blood detection result and the blood cell detection result to obtain a second immune detection result includes: calculating to obtain a second immune detection result by adopting the following formula; wherein A is the whole blood detection result, and HCT is the blood cell detection result.

The application adopts another technical scheme that: there is provided an immunoassay analyzer comprising a processor and a memory interconnected, the memory for storing program data, the processor for executing the program data to implement a method as described above.

The application adopts another technical scheme that: there is provided a computer readable storage medium having stored therein program data which, when executed by a processor, is adapted to carry out a method as described above.

The detection method of the immunoassay analyzer provided by the application comprises the following steps: obtaining a sample to be detected; if the sample to be detected is a serum sample or a plasma sample, inputting the sample to be detected into an immunoassay detection channel for detection, and obtaining a first immunoassay result; if the sample to be detected is a whole blood sample, inputting part of the sample to be detected into an immunoassay detection channel for detection to obtain a whole blood detection result, inputting part of the sample to be detected into an HCT calculation channel to obtain a blood cell detection result, and calculating to obtain a second immunoassay result according to the whole blood detection result and the blood cell detection result. Through the mode, the serum sample, the plasma sample and the whole blood sample can be subjected to immunoassay detection on the same immunoassay analyzer, so that the equipment cost is saved, and the detection efficiency is improved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:

FIG. 1 is a first perspective view of the interior of a first embodiment of an immunoassay analyzer provided by the present application;

FIG. 2 is a second perspective view of the interior of the first embodiment of the immunoassay device provided by the present application;

FIG. 3 is a schematic perspective view of an impedance cell assembly according to a first embodiment of the immunoassay device of the present application;

FIG. 4 is a schematic perspective view of a cuvette assembly according to a first embodiment of an immunoassay device provided by the present application;

FIG. 5 is a flow chart of an embodiment of a detection method of an immunoassay analyzer according to the present application;

FIG. 6 is a schematic perspective view of an automatic sample injection device according to the present application;

FIG. 7 is a schematic perspective view of a partial assembly of the autosampler of FIG. 6;

FIG. 8 is a schematic perspective view of a partial assembly of the autosampler of FIG. 6;

FIG. 9 is a schematic side elevational view of various sample tubes;

FIG. 10 is a first flow chart of step 52 of FIG. 9;

FIG. 11 is a second flow chart of step 52 of FIG. 9;

FIG. 12 is a third flow chart of step 52 of FIG. 9;

FIG. 13 is a flow chart of an embodiment of impedance detection according to the present application;

FIG. 14 is a flow chart of an embodiment of colorimetric detection provided by the present application;

FIG. 15 is a schematic view showing the structure of a second embodiment of an immunoassay device according to the present application;

fig. 16 is a schematic structural diagram of an embodiment of a computer readable storage medium provided by the present application.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. It is to be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the application. It should be further noted that, for convenience of description, only some, but not all of the structures related to the present application are shown in the drawings. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

The terms "first," "second," and the like in this disclosure are used for distinguishing between different objects and not for describing a particular sequential order. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed steps or elements but may include other steps or elements not listed or inherent to such process, method, article, or apparatus.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.

Referring to fig. 1 to 4 together, fig. 1 is a first perspective view of the interior of a first embodiment of an immunoassay device according to the present application, fig. 2 is a second perspective view of the interior of the first embodiment of the immunoassay device according to the present application, fig. 3 is a perspective view of an impedance cell assembly of the first embodiment of the immunoassay device according to the present application, and fig. 4 is a perspective view of a cuvette assembly of the first embodiment of the immunoassay device according to the present application.

The application provides an immunoassay analyzer, which comprises a shell, a frame, a reaction disk assembly 410, a sample needle assembly 420, a reagent storage disk 430, a reagent needle assembly 440, a first cleaning tank 442, an impedance tank assembly 450 (or a cuvette assembly 550), a sample feeding mechanism, a cup feeding mechanism 460, a stirring mechanism 470, a second cleaning tank 472, a magnetic separation mechanism 480, an optical detection module, a measuring needle assembly 490, a cup grabbing mechanism 492, a cup waste slideway 494 and a cup waste bin 496. To show the internal structure of the immunoassay device, the housing has been removed in fig. 1 and 2.

The reaction disk assembly 410 is disposed on the frame, and the reaction disk assembly 410 is used for loading and transferring the reaction cup.

The sample needle assembly 420 is disposed on the frame for reciprocating movement in a first direction to sample and divide the sample, and the sample needle assembly 420 is disposed above the reaction disk assembly 410 to divide the sample into reaction cups. The sample needle assembly 420 is provided with four stations of an open sample injection and sample suction position, an impedance cell or cuvette sample addition position, an automatic sample injection and sample suction position and a reaction cup sample addition position in the horizontal direction, wherein the open sample injection and sample suction position can support an operator to directly hold a sample tube for the sample needle assembly 420 to suck samples, and the automatic sample injection and sample suction position is a sampling station fixed on a sample injection mechanism and is positioned at the intersection point position of the sample needle assembly 420 and the sample injection mechanism.

A reagent storage tray 430 is provided on the housing and at a side of the reaction tray assembly 410 for refrigerated storage of reagents, which may be one, two or more. The reaction disk assembly 410 and the reagent storage disk 430 may be located on the left and right sides of the immunoassay analyzer, respectively.

A reagent needle assembly 440 is pivotally mounted to the housing and positioned between the reaction disk assembly 410 and the reagent storage disk 430 for accessing the reagent and adding it to the reaction cup. The swing track of the reagent needle assembly 440 is disposed to intersect the reaction disk assembly 410 and the reagent storage disk 430. The reagent needle assembly 440 may be provided with four stations on its circumferential swing track, namely a first reagent station, a second reagent station, a cleaning station, and a reagent filling station.

The impedance cell assembly 450 (or cuvette assembly 550) is positioned below the path of movement of the sample needle assembly 420.

Wherein, when the sample is a serum sample or a plasma sample, the cuvette receives the sample needle assembly 420 for sampling, and when the sample is a whole blood sample, the cuvette, the impedance cell assembly 450 (or the cuvette assembly 550) each receives the sample needle assembly 420 for sampling.

The cup feeding mechanism 460 is arranged on the frame and is positioned above the reaction disk assembly 410, and the cup feeding mechanism 460 is used for placing the reaction cup and conveying the reaction cup to the reaction disk assembly 410 in a preset posture after shaping.

The automatic sample injection device is arranged at the front side of the rack, and is used for loading the sample rack so that the sample tubes on the rack pass through the sampling stations one by one in the second direction, and specifically comprises a sample injection mechanism 300, a first sensor 310, a second sensor 320, a third sensor 330, a fourth sensor 340, a code scanner, a rotating mechanism 100 and a shaking mechanism 350.

The sample introduction mechanism 300 is used to transport a sample rack through a sampling station, and the impedance cell assembly 450 (or cuvette assembly 550) may be provided on the sample introduction mechanism 300 or on the rack. The impedance cell assembly 450 (or the cuvette assembly 550) is arranged on the sample injection mechanism 300, so that maintenance can be conveniently performed, and the impedance cell assembly 450 (or the cuvette assembly 550) is arranged on the rack, so that the structure of the immunoassay analyzer is more compact.

A first sensor 310 is provided on the sample introduction mechanism 300 for detecting whether there is a sample tube on the sample rack at a first height position.

A second sensor 320 is provided on the sample introduction mechanism 300 for detecting the height type of the sample tube on the sample rack at a second height position, which is higher than the first height position.

And a third sensor 330 provided on the sample introduction mechanism 300 for detecting the height type of the sample tube on the sample rack at a third height position higher than the second height position.

Wherein the sampling station is downstream of the first sensor 310, the second sensor 320, and the third sensor 330.

A fourth sensor 340 is provided on the sample introduction mechanism 300 for detecting the shape type of the sample tube on the sample rack at a fourth height position, which is lower than the first height position, and the sampling station is located downstream of the fourth sensor 340.

The code scanner is disposed on the frame and below the sample needle assembly 420. The rotating mechanism 100 is arranged on the sample feeding mechanism 300 and is positioned at the front side of the sampling station, the rotating mechanism 100 is used for rotating the sample tube, the rotating mechanism 100 comprises a transmission piece and a driving motor, the driving motor is used for driving the transmission piece to rotate, and the linked sample tube rotates on the basis of the axis of the sample tube so as to facilitate the code scanner to shoot the identification code on the sample tube. Reference is made to the foregoing embodiments for specific construction of the rotation mechanism 100.

The shaking mechanism 350 is disposed on the frame, the shaking mechanism 350 is used for grabbing a sample tube and shaking the sample tube, and the shaking mechanism 350 is disposed between the third sensor 330 and the sampling station. Other specific structures of the autosampler may refer to the previous embodiments.

The reagent storage disk 430 is provided with a plurality of reagent stations on the swing trajectory of the reagent needle assembly 440, or the reagent storage disk 430 can spin a plurality of reagents to the reagent stations.

The reaction disk assembly 410 includes a heat-insulating pot for providing constant temperature incubation, and a turntable provided with a plurality of reaction cup holes in a circumferential direction to drive the reaction cups to circulate.

The immunoassay analyzer further comprises a stirring mechanism 470, wherein the stirring mechanism 470 is rotatably arranged on the frame and positioned at the side edge of the reaction disk assembly 410, and is used for uniformly mixing the sample liquid in the reaction cup, and the stirring mechanism 470 comprises one or more stirring paddles capable of rotating and autorotating. The stirring paddle can be flat, and plays a role in stirring and uniformly mixing during autorotation.

The first cleaning tank 442 is disposed at a side of the reagent storage tray 430 and located on a swing track of the reagent needle assembly 440 to perform reagent needle cleaning after the reagent needle assembly 440 takes reagent, and the second cleaning tank 472 is disposed at a side of the stirring mechanism 470 away from the reaction tray assembly 410 and located below the swing track of the stirring mechanism 470 to perform stirring paddle cleaning after stirring of the stirring paddles is completed.

The magnetic separation mechanism 480 is disposed on the frame and located at a side of the reaction disk assembly 410, and the magnetic separation mechanism 480 includes a magnetic separation disk, a liquid injection mechanism, and a liquid suction mechanism, and is used for magnetically separating and cleaning a sample liquid to be tested.

The optical detection module (not shown) is disposed on the rack and is erected above the reagent disk assembly, the measurement needle assembly 490 is erected above the magnetic separation mechanism 480, the measurement needle assembly 490 comprises a measurement mixing mechanism and a measurement needle mechanism, the measurement mixing mechanism is used for mixing the reacted sample liquid, and the measurement needle mechanism sucks the mixed sample liquid to the optical detection module for flow fluorescence detection.

The cup grabbing mechanism 492, the waste cup slideway 494 and the waste cup bin 496, wherein the cup grabbing mechanism 492 is arranged on the frame and is arranged above the magnetic separation mechanism 480, and is used for taking out the reaction cup sampled by the measuring needle mechanism and placing the reaction cup on the waste cup slideway to enter the waste cup bin 496.

As shown in fig. 3, the impedance cell assembly 450 includes a metal shield case 451, a counter cell, a syringe (not shown), and a pressure chamber (not shown).

The metal shield case 451 is provided with a sample introduction hole 452. The counting cell is arranged in the metal shielding box 451 and aligned with the sample inlet. The injector is fixed through the frame and is used for inputting the reagent into the counting cell or uniformly mixing by bubbling. The pressure chamber is fixed through the frame and is used for providing negative pressure so that particles to be detected in the sample liquid to be detected in the counting cell linearly flow.

Specifically, the impedance cell assembly 450 further includes a fill tube 453, an isolation chamber 454, a metal shield 456, a first cell 457, a second cell 458, a first electrode, and a second electrode.

The liquid filling pipe 453 penetrates the metal shielding case 451 to feed a reagent into the counting chamber. The isolation chamber 454 is arranged in the metal shielding box 451 and is positioned below the counting cell, and the isolation chamber 454 has a certain volume for gas-liquid isolation, wherein the isolation chamber 454 is used for receiving the detected waste liquid and also used for transferring the gas flow for bubbling and uniformly mixing.

The metal shielding tube 456 is connected to the metal shielding case 451 to form a grounding effect for switching the flexible reagent line, so as to prevent the sample liquid in the flexible reagent line from interfering with the electrical signal.

Specifically, the counting cell includes a jewel hole, a first cell 457, a second cell 458, a first electrode, and a second electrode.

The jewel hole is used for allowing particles to be measured to pass through one by one. The first well 457 is disposed on a first side of the gemstone aperture for receiving a reagent and whole blood sample. The second cell 458 is disposed on a second side of the jewel hole and is in communication with the first cell 457 via the jewel hole. The first electrode and the second electrode are arranged on the side edge of the jewel hole and are used for receiving electric signals when particles to be detected pass through. Specifically, a constant electric field is generated in the jewel hole of the first electrode and the second electrode, when the sample liquid in the first tank body 457 is pumped through the jewel hole by the negative pressure provided by the pressure chamber, a pulse signal appears in the cell particles through the electric field, and the RBC (red blood cell number) parameter and the MCV (average red blood cell volume) parameter can be obtained through analyzing the pulse size and the number, so that the HCT (red blood cell packed volume) parameter is calculated through hct=rbc.

When the sample is a serum sample or a plasma sample, the computer system analyzes the detection data of the optical detection module and directly reports the result, and the result is marked as A;

When a whole blood sample is obtained, the computer system analyzes, calculates and reports the data of the optical detection module and the data of the impedance cell assembly 450, and the result is denoted as B. Wherein,

As shown in fig. 4, cuvette assembly 550 includes a metal shield 551, a fill tube 553, and a cuvette 554.

The metal shielding box 551 is provided with a sample injection hole 552, and a liquid injection pipe 553 penetrates the metal shielding box 551 and is used for inputting a reagent into the cuvette 554, the cuvette 554 is arranged in the metal shielding box 551 and aligned with the sample injection hole 552, and the optical detection assembly 555 is arranged in the metal shielding box 551 and is positioned at two sides of the cuvette 554 for optical detection.

The HCT counting mode of the cuvette is: the HGB (hemoglobin) number and MCHC (mean hemoglobin concentration) were obtained by measuring the absorbance of the solution to a laser light of 554.+ -.20 nm wavelength. From the following componentsHCT values can be obtained.

Referring to fig. 5, fig. 5 is a flow chart of an embodiment of a detection method of an immunoassay analyzer according to the present application, the method includes:

Step 51: and obtaining a sample to be detected.

Optionally, before step 51, the method further includes: judging whether a sample rack is detected; if yes, detecting the type of the sample container on the sample rack; and detecting the type of the sample to be detected in the corresponding sample container according to the type of the sample container.

The type of the sample container on the sample rack can be detected by the following modes: detecting whether a sample tube exists on the sample rack at a first height position by adopting a first sensor; and detecting a height type of the sample tube on the sample rack at a second height position, which is higher than the first height position, using the second sample sensor; detecting the height type of the sample tube on the sample rack at a third height position by using a third sensor, wherein the third height position is higher than the second height position; the type of the sample container on the sample rack is determined according to the detection results of the first sensor, the second sensor and the third sensor.

In addition, a fourth sensor can be further arranged, the shape type of the sample tube on the sample rack is detected at a fourth height position by the fourth sensor, the fourth height position is lower than the first height position, and the type of the sample container on the sample rack is determined according to detection results of the first sensor, the second sensor, the third sensor and the fourth sensor.

Referring to fig. 6 to fig. 9 together, fig. 6 is a schematic perspective view of an automatic sample injection device according to the present application; FIG. 7 is a schematic perspective view of a partial assembly of the autosampler of FIG. 6; FIG. 8 is a schematic perspective view of a partial assembly of the autosampler of FIG. 6; fig. 9 is a schematic side view of a sample tube, and the present embodiment further provides a sample tube identification device, which includes a first sensor 310, a second sensor 320, and a third sensor 330.

The first sensor 310 is configured to detect whether there is a sample tube on the sample rack at a first height position, which may be slightly higher than the rack; the second sensor 320 is used to detect the height type of the sample tube on the sample rack at a second height position, which is higher than the first height position; the third sensor 330 is used to detect the height type of the sample tube on the sample rack at a third height position, which is higher than the second height position. The second sensor 320, the third sensor 330 can be used to identify whether the normal sample tube of two different heights (e.g., a sample tube of 100mm in height and a sample tube of 75mm in height) on the left side in fig. 12 is capped.

In an alternative embodiment, the sample tube identification device further comprises a fourth sensor 340, the fourth sensor 340 being adapted to detect the shape type of the sample tube on the sample rack at a fourth height position, the fourth height position being lower than the first height position. The fourth sensor 340 may be used to identify a particular sample tube on the right in fig. 12. If the fourth sensor 340 is not provided, the information of the pipe can be input through a software interface of the instrument to identify, or the fourth sensor 340 and the software interface can be provided to judge simultaneously, so that the reliability is improved.

In the embodiment of the present invention, the second sensor 320 and the third sensor 330 are correlation sensors. Specifically, the second sensor 320 and the third sensor 330 may be optocouplers, each including a transmitting end and a receiving end, and whether the sample tube is capped or not may be determined according to the light receiving state of the receiving end.

In an actual product, the first sensor 310, the second sensor 320, the third sensor 330, and the fourth sensor 340 are aligned in a vertical direction so that the product is compact.

The first sensor 310, the second sensor 320, the third sensor 330 and the fourth sensor 340 may also be arranged in a deviated manner in the vertical direction to form a plurality of recognition stations in a linear distribution, so that maintenance is facilitated.

As shown in fig. 7, the second sensor 320 and the third sensor 330 are aligned in the vertical direction. The first sensor 310 and the fourth sensor 340 are disposed on both left and right sides of the second sensor 320 and the third sensor 330.

As shown in fig. 7, the sample tube identification device further comprises a first mounting frame 301, which may be arched, comprising two mounting arms arranged at intervals, and the second sensor 320 and the third sensor 330 are arranged at different height positions of the mounting arms.

As shown in fig. 8, the sample tube identification device further comprises a second mounting frame 302, and the first sensor 310 and/or the fourth sensor 340 are disposed on the second mounting frame 302.

The invention also provides an automatic sample injection device, which comprises the sample tube identification device.

The automatic sample injection device further comprises a rotating mechanism and a code scanner, the rotating mechanism is used for rotating the sample tube, the rotating mechanism comprises a transmission piece and a driving motor, the driving motor is used for driving the transmission piece to rotate so as to link the sample tube to conduct autorotation based on the axis of the sample tube, and the code scanner is convenient to shoot the identification code on the sample tube. For a specific structure of the rotation mechanism, reference may be made to the rotation mechanism 100 in the foregoing embodiment.

The application also provides an automatic sample injection device, which comprises a sample injection mechanism 300, a first sensor 310, a second sensor 320 and a third sensor 330.

The sample feeding mechanism 300 is used for conveying sample racks to pass through the sampling station 123, the sample feeding mechanism 300 comprises an X-direction sample feeding mechanism and a Y-direction sample feeding mechanism, the X-direction sample feeding mechanism is responsible for conveying the sample racks placed on the sample feeding mechanism 300 to a Y-direction sample feeding channel, and the Y-direction sample feeding mechanism is responsible for sequentially conveying the sample racks to each operation station along the Y-direction sample feeding channel according to fixed steps. An opposite-injection sensor (a side area or other sensors) is arranged in the middle area of the X-direction sample injection mechanism, and a switch or a sensor is arranged at the starting position of the Y-direction sample injection channel; the first sensor 310 is configured to detect whether there is a sample tube on the sample rack at a first height position, which may be slightly higher than the rack; the second sensor 320 is used to detect the height type of the sample tube on the sample rack at a second height position, which is higher than the first height position; the third sensor 330 is used to detect the height type of the sample tube on the sample rack at a third height position, which is higher than the second height position, and the second sensor 320 and the third sensor 330 can be used to identify the common sample tube of two different heights (e.g. a sample tube of 100mm in height and a sample tube of 75mm in height) on the left side in fig. 7 and whether the cap is present or not; wherein the sampling station 123 is located downstream of the first sensor 310, the second sensor 320, and the third sensor 330, i.e. the identification of the sample tube is completed before sampling.

In an alternative embodiment, the sample tube identification device further comprises a fourth sensor 340, the fourth sensor 340 being adapted to detect the shape type of the sample tube on the sample rack at a fourth height position, the fourth height position being lower than the first height position. The fourth sensor 340 may be used to identify a particular sample tube on the right in fig. 7. If the fourth sensor 340 is not provided, the information of the pipe can be input through a software interface of the instrument to identify, or the fourth sensor 340 and the software interface can be provided to judge simultaneously, so that the reliability is improved.

In the embodiment of the present invention, the second sensor 320 and the third sensor 330 are correlation sensors. Specifically, the second sensor 320 and the third sensor 330 may be optocouplers, each including a transmitting end and a receiving end, and whether the sample tube is capped or not may be determined according to the light receiving state of the receiving end

The first sensor 310, the second sensor 320, the third sensor 330 and the fourth sensor 340 may also be arranged in a deviated manner in the vertical direction to form a plurality of recognition stations in a linear distribution, so that the maintenance is convenient.

As shown in fig. 7, the sample tube identification device further comprises a second mounting frame 302, and the first sensor 310 and/or the fourth sensor 340 are disposed on the second mounting frame 302.

The automatic sample injection device also comprises a rotating mechanism and a code scanner, wherein the rotating mechanism is used for rotating the sample tube. The rotating mechanism comprises a transmission part and a driving motor, and the driving motor is used for driving the transmission part to rotate so as to link the sample tube to conduct autorotation based on the axis of the sample tube, so that the code scanner can conveniently shoot the identification code on the sample tube. For a specific structure of the rotation mechanism, reference may be made to the rotation mechanism 100 in the foregoing embodiment.

In addition, the automatic sample injection device further includes a shaking mechanism 350, the shaking mechanism 350 is used for grabbing a sample tube and shaking the sample tube, and the shaking mechanism 350 is disposed between the third sensor 330 and the sampling station 123 and can be disposed at the same station as the fourth sensor 340.

In the embodiment of the present invention, the shaking mechanism 350 may be omitted, and the mixing effect may be achieved by the high-speed rotation of the rotation mechanism 100. Or the rotating mechanism 100 is omitted, and the identification codes of the sample tubes are uniformly oriented on the sample rack and can be shot by the code scanner.

The control flow of the automatic sample injection device provided by the embodiment of the invention is as follows:

When a correlation sensor (or other sensors) arranged on the X-direction sampling mechanism detects that a sample is put in, the X-direction sampling mechanism conveys the sample to be tested to the Y-direction sampling channel along the X direction, and when the sample frame touches or approaches a switch or a sensor at the starting position of the Y-direction sampling channel, the Y-direction sampling mechanism starts to start.

The Y-direction sample feeding mechanism conveys the sample rack to a sample tube presence or absence detection station along the Y-direction sample feeding path, and the first sensor 310 performs a first-step detection on the type of the sample tube on the sample rack.

The Y-direction sample feeding mechanism conveys the sample rack to a high-low sample tube detection station along the Y-direction sample feeding channel, and the second sensor 320 and the third sensor 330 which are respectively arranged at the upper position and the lower position detect the sample tube in the second step.

The Y-direction sample feeding mechanism conveys the sample rack to a code scanning station along the Y-direction sample feeding channel, the rotating mechanism drives the sample tube to rotate, and a scanner (not shown) arranged at the station scans and identifies sample information on the sample tube. If the rotating mechanism is not arranged, the step is canceled, and correspondingly, the sample tubes are required to be regularly arranged so that the identification codes are opposite to the code scanner.

The Y-direction sample feeding mechanism conveys the sample rack to the shaking station along the Y-direction sample feeding channel, the X-direction moving mechanism of the shaking mechanism 350 moves along the X-direction, after the sample tube is clamped, the Z-direction moving mechanism of the shaking mechanism 350 lifts the clamped sample tube to a shaking position along the Z-direction, and the shaking mechanism shakes the sample to be measured. After thoroughly mixing, the sample tube is returned to its original position. If the shaking mechanism 350 is not arranged, the step is canceled, and correspondingly, the shaking mechanism rotates at a high speed to achieve the mixing effect.

The Y-direction sampling mechanism conveys the sample rack to the auto-sampling station 123 along the Y-direction sampling channel, a sample needle assembly (not shown) disposed directly above horizontally moves to the auto-sampling station 123, and then the sample needle sampling mechanism descends to a sample sucking position along the vertical direction to suck samples. After the sample is sucked, the sample needle sampling mechanism returns to the initial position in the vertical direction, and the next operation is ready.

The Y-direction sample feeding mechanism conveys the sample rack to the exit area along the Y-direction sample feeding channel, and when the sample rack touches or approaches to an end switch or a sensor of the Y-direction sample discharging channel, the swing mechanism ejects the sample rack.

The sample tube identification device provided by the invention has a simple structure, can conveniently identify multiple types of sample tubes, and solves the problem that medical staff need to regularly place the similar sample tubes.

Step 52: if the sample to be detected is a serum sample or a plasma sample, the sample to be detected is input into an immunoassay detection channel for detection, and a first immunoassay result is obtained.

Optionally, as shown in fig. 10, fig. 10 is a first flowchart of step 52 in fig. 9, where step 52 may specifically include:

step 52a1: a first reagent is added to the target cuvette.

Referring to fig. 1 and 2 described above, the reagent needle assembly 440 is controlled to travel to a first reagent station of the reagent storage tray 430, draw a first reagent from the first reagent station, then rotate to a reagent filling station of the reaction tray assembly 410, and fill a first reagent into a target reaction cup in the reaction tray assembly 410.

Step 52a2: and adding a sample to be detected into the target reaction cup.

Referring to fig. 1 and 2, the reaction cup to which the first reagent has been added in the reaction disk assembly 410 rotates with the reaction disk assembly 410 to the sample adding station, the sample needle assembly 420 moves to the sample sucking station of the automatic sample feeding assembly, and the sample to be measured is sucked from the sample tube and then moved to the sample adding station of the reaction disk assembly 410 for sample adding.

Step 52a3: and sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup.

With reference to fig. 1 and 2 described above:

and (3) uniformly mixing: the reaction cup with the first reagent and sample added to the reaction plate assembly 410 rotates with the reaction plate assembly 410 to the stirring station, and the stirring mechanism 470 stirs and mixes the reactants.

Incubation operation: the reactants, which have been stirred and mixed in the reaction tray assembly 410, are incubated at 37 ℃ or a set target temperature for a certain period of time, and then the cuvette carrying the reaction that has completed the first stage reaction is transferred by the cuvette handling mechanism 492 to the magnetic separation mechanism 480.

Magnetic separation operation: the magnetic separation mechanism 480 performs magnetic separation on reactants in the reaction cup, the target cup position rotates to the liquid injection station after a certain time of magnetic separation, the liquid injection mechanism injects cleaning liquid into the reaction cup, then the turntable drives the target cup position to rotate to the liquid suction station, the liquid suction mechanism removes supernatant liquid of the reaction cup after a certain time of magnetic separation (the process can be performed only once or multiple times according to actual requirements), the turntable drives the target cup position to rotate to the liquid injection station of the measuring solution, the liquid injection needle special for the measuring solution injects quantitative solution into the target cup position, finally the turntable drives the target cup position to rotate to the cup discharge station of the magnetic separation disc, and the cup grabbing mechanism 492 transfers the target cup position to the measuring and mixing mechanism.

Step 52a4: and detecting the liquid in the target reaction cup, and obtaining a first immune detection result.

Specifically, uniformly mixing the liquid in the target reaction cup; inputting the liquid in the target reaction cup to an optical detection module so that the optical detection module detects the liquid and a first immune detection result is obtained.

Optionally, as shown in fig. 11, fig. 11 is a second flow chart of step 52 in fig. 9, and step 52 may specifically include:

step 52b1: a first reagent is added to the target cuvette.

Step 52b2: and adding a sample to be detected into the target reaction cup.

Step 52b3: and sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup.

The foregoing steps are similar to those of the first embodiment described above, except that: after the magnetic separation, the turntable rotates the target cup position to the cup ejection position of the magnetic separation mechanism 480, and the cup gripping mechanism 492 transfers the target cup position back to the reaction disk assembly 410, since the second reagent addition is performed.

Step 52b4: a second reagent is added to the target cuvette.

Step 52b5: and (3) sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup for the second time.

Step 52b6: and detecting the liquid in the target reaction cup, and obtaining a first immune detection result.

Optionally, as shown in fig. 12, fig. 12 is a third flow chart of step 52 in fig. 9, and step 52 may specifically include:

Step 52c1: a first reagent is added to the target cuvette.

Step 52c2: and adding a sample to be detected into the target reaction cup.

Step 52c3: and sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup.

Step 52c4: a second reagent is added to the target cuvette.

Step 52c5: and (3) sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup for the second time.

After the second magnetic separation, the turntable rotates the target cup position to the cup discharge station of the magnetic separation mechanism 480, and the cup grasping mechanism 492 transfers the target cup position back to the reaction disk assembly 410.

Step 52c6: a third reagent is added to the target cuvette.

Step 52c7: and (3) sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup for the third time.

Step 52c8: and detecting the liquid in the target reaction cup, and obtaining a first immune detection result.

It will be appreciated that the above three embodiments correspond to a one-step method, a two-step method and a three-step method, respectively, and that in actual operation, different step flows may be performed according to the types of reagents or manual selection.

Step 53: if the sample to be detected is a whole blood sample, inputting part of the sample to be detected into an immunoassay detection channel for detection to obtain a whole blood detection result, inputting part of the sample to be detected into an HCT calculation channel to obtain a blood cell detection result, and calculating to obtain a second immunoassay result according to the whole blood detection result and the blood cell detection result.

The part of the sample to be detected is input into the immunoassay detection channel for detection, and the detection steps of obtaining the whole blood detection result on the serum sample or the plasma sample are consistent, which is not described herein.

Referring to fig. 13, fig. 13 is a flowchart of an embodiment of impedance detection according to the present application, where the method includes:

Step 131: a diluent is added to the impedance detection cell.

Step 132: and adding a sample to be detected into the impedance detection cell.

Step 133: and uniformly mixing the liquid in the impedance detection tank.

Step 134: and detecting the liquid after the uniform mixing operation in the impedance detection pool, and obtaining a blood cell detection result.

Specifically, step 134 may specifically be: detecting the number RBC and average red blood cell volume MCV of a sample to be detected; the hematocrit HCT was calculated as a blood cell test result using the following formula:

HCT＝RBC*MCV。

Wherein, according to the whole blood detection result and the blood cell detection result, a second immune detection result is obtained by calculation, specifically: calculating to obtain a second immune detection result by adopting the following formula;

wherein A is the whole blood detection result, and HCT is the blood cell detection result.

Referring to fig. 14, fig. 14 is a flow chart of an embodiment of colorimetric detection provided in the present application, where the method includes:

Step 141: adding a hemolytic agent into the colorimetric detection cell.

Step 142: and adding a sample to be detected into the colorimetric detection pool.

Step 143: and (5) uniformly mixing the liquid in the contrast color detection pool.

Step 144: detecting the liquid after the uniform mixing operation in the colorimetric detection pool, and obtaining a blood cell detection result.

Specifically, step 134 may specifically be: detecting the quantity HGB and the average hemoglobin concentration MCHC of a sample to be detected; the hematocrit HCT was calculated as a blood cell test result using the following formula:

Different from the prior art, the detection method of the immunoassay analyzer provided by the application comprises the following steps: obtaining a sample to be detected; if the sample to be detected is a serum sample or a plasma sample, inputting the sample to be detected into an immunoassay detection channel for detection, and obtaining a first immunoassay result; if the sample to be detected is a whole blood sample, inputting part of the sample to be detected into an immunoassay detection channel for detection to obtain a whole blood detection result, inputting part of the sample to be detected into an HCT calculation channel to obtain a blood cell detection result, and calculating to obtain a second immunoassay result according to the whole blood detection result and the blood cell detection result. Through the mode, the serum sample, the plasma sample and the whole blood sample can be subjected to immunoassay detection on the same immunoassay analyzer, so that the equipment cost is saved, and the detection efficiency is improved.

Referring to fig. 15, fig. 15 is a schematic structural diagram of a second embodiment of an immunoassay analyzer provided by the present application, the immunoassay analyzer includes a processor 151 and a memory 152, wherein the memory 152 stores program data, and the processor 151 is configured to execute the program data to implement the following method:

obtaining a sample to be detected; if the sample to be detected is a serum sample or a plasma sample, inputting the sample to be detected into an immunoassay detection channel for detection, and obtaining a first immunoassay result; if the sample to be detected is a whole blood sample, inputting part of the sample to be detected into an immunoassay detection channel for detection to obtain a whole blood detection result, inputting part of the sample to be detected into an HCT calculation channel to obtain a blood cell detection result, and calculating to obtain a second immunoassay result according to the whole blood detection result and the blood cell detection result.

Referring to fig. 16, fig. 16 is a schematic structural diagram of an embodiment of a computer readable storage medium provided in the present application, where the computer readable storage medium 160 stores program data 161, and the program data 161, when executed by a processor, is configured to implement the following method steps:

In an embodiment of the immunoassay analyzer and the computer readable storage medium described above, the program data, when executed by the processor, is further for implementing the steps of:

Judging whether a sample rack is detected; if yes, detecting the type of the sample container on the sample rack; and detecting the type of the sample to be detected in the corresponding sample container according to the type of the sample container.

Optionally, it is further used to implement: detecting whether a sample tube exists on the sample rack at a first height position by adopting a first sensor; and detecting a height type of the sample tube on the sample rack at a second height position, which is higher than the first height position, using the second sample sensor; detecting the height type of the sample tube on the sample rack at a third height position by using a third sensor, wherein the third height position is higher than the second height position; the type of the sample container on the sample rack is determined according to the detection results of the first sensor, the second sensor and the third sensor.

Optionally, it is further used to implement: a fourth sensor is combined with the first sensor, the second sensor and the third sensor, and the type of the sample tube on the sample rack is confirmed at a fourth height position, wherein the fourth height position is lower than the first height position; determining the type of the sample container on the sample rack according to the detection results of the first sensor, the second sensor and the third sensor comprises: the type of the sample container on the sample rack is determined according to the detection results of the first sensor, the second sensor, the third sensor and the fourth sensor.

Optionally, it is further used to implement: adding a first reagent into a target reaction cup; adding a sample to be detected into a target reaction cup; sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup; and detecting the liquid in the target reaction cup, and obtaining a first immune detection result.

Optionally, it is further used to implement: adding a second reagent into the target reaction cup; and (3) sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup for the second time.

Optionally, it is further used to implement: adding a third reagent into the target reaction cup; and (3) sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup for the third time.

Optionally, it is further used to implement: sequentially and uniformly mixing the liquid in the target reaction cup; placing the target reaction cup in a set temperature environment for incubation operation for a set time length; and performing magnetic separation operation on the liquid in the target reaction cup.

Optionally, it is further used to implement: uniformly mixing the liquid in the target reaction cup; inputting the liquid in the target reaction cup to an optical detection module so that the optical detection module detects the liquid and a first immune detection result is obtained.

Optionally, it is further used to implement: adding a diluent into the impedance detection cell; adding a sample to be detected into the impedance detection pool; uniformly mixing the liquid in the impedance detection pool; and detecting the liquid after the uniform mixing operation in the impedance detection pool, and obtaining a blood cell detection result.

Optionally, it is further used to implement: detecting the number RBC and average red blood cell volume MCV of a sample to be detected; the hematocrit HCT was calculated as a blood cell test result using the following formula: hct=rbc MCV.

Calculating to obtain a second immune detection result by adopting the following formula; wherein A is the whole blood detection result, and HCT is the blood cell detection result.

Optionally, it is further used to implement: adding a hemolytic agent into the impedance detection cell; adding the sample to be detected into the impedance detection pool; uniformly mixing the liquid in the impedance detection tank; and detecting the liquid after the uniform mixing operation in the impedance detection pool, and obtaining a blood cell detection result.

Optionally, it is further used to implement: detecting the hemoglobin quantity HGB and the average hemoglobin concentration MCHC of the sample to be detected; the hematocrit HCT was calculated as a blood cell test result using the following formula:

Optionally, it is further used to implement: calculating to obtain a second immune detection result by adopting the following formula; wherein A is the whole blood detection result, and HCT is the blood cell detection result.

In the several embodiments provided in the present application, it should be understood that the disclosed method and apparatus may be implemented in other manners. For example, the above-described device embodiments are merely illustrative, e.g., the division of the modules or units is merely a logical functional division, and there may be additional divisions when actually implemented, e.g., multiple units or components may be combined or integrated into another system, or some features may be omitted, or not performed.

The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the embodiment.

In addition, each functional unit in each embodiment of the present application may be integrated in one processing unit, each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in software functional units.

The foregoing description is only of embodiments of the present application, and is not intended to limit the scope of the application, and all equivalent structures or equivalent processes according to the present application and the accompanying drawings, or direct or indirect application in other related technical fields, are included in the scope of the present application.

Claims

1. A detection method of an immunoassay analyzer, applied to an immunoassay analyzer, characterized in that the immunoassay analyzer comprises:

A frame;

The reaction disc assembly is arranged on the frame and is used for loading reaction cups and circulating the reaction cups;

The sample needle assembly is arranged on the rack and used for reciprocating motion in a first direction so as to sample and divide samples;

The reagent storage disc is arranged on the frame and positioned at the side edge of the reaction disc assembly and is used for refrigerating and storing one, two or more reagents;

The reagent needle assembly is arranged on the rack in a swinging way and is positioned between the reaction disk assembly and the reagent storage disk and used for taking the reagent and adding the reagent into the reaction cup; the reagent storage disc is provided with a plurality of reagent stations on the swing track of the reagent needle assembly, or can rotate a plurality of reagents to the reagent stations;

The magnetic separation mechanism is arranged on the rack and positioned at the side edge of the reaction disk assembly, and the magnetic separation mechanism is used for carrying out magnetic separation on the sample liquid to be detected;

the optical detection module is arranged on the rack and is erected above the reagent disk assembly;

the measuring needle assembly is erected above the magnetic separation mechanism and comprises a measuring and mixing mechanism and a measuring needle mechanism, the measuring and mixing mechanism is used for mixing the reacted sample liquid uniformly, and the measuring needle mechanism absorbs the mixed sample liquid to the optical detection module for flow fluorescence detection;

An impedance tank assembly;

The method comprises the following steps:

judging whether a sample rack is detected;

if yes, detecting whether a sample tube exists on the sample rack at a first height position by adopting a first sensor; and

Detecting a height type of a sample tube on the sample rack at a second height position with a second sensor, the second height position being higher than the first height position;

Detecting a height type of a sample tube on the sample rack at a third height position with a third sensor, the third height position being higher than the second height position;

determining the type of the sample container on the sample rack according to the detection results of the first sensor, the second sensor and the third sensor;

Detecting the type of a sample to be detected in the corresponding sample container according to the type of the sample container;

Obtaining a sample to be detected;

If the sample to be detected is a serum sample or a plasma sample, inputting the sample to be detected into an immunoassay detection channel for detection, and obtaining a first immunoassay result; wherein the immunoassay detection passageway comprises the reaction disk assembly, a magnetic separation mechanism, a measurement needle assembly and the optical detection module;

If the sample to be detected is a whole blood sample, inputting part of the sample to be detected into the immunoassay detection channel for detection to obtain a whole blood detection result, inputting part of the sample to be detected into the HCT calculation channel to obtain a blood cell detection result, and calculating to obtain a second immunoassay result according to the whole blood detection result and the blood cell detection result; wherein the HCT calculation channel includes an impedance cell assembly.

2. The method of claim 1, wherein the step of determining the position of the substrate comprises,

The method further comprises the steps of:

Identifying the type of sample tube on the sample rack at a fourth elevation position, which is lower than the first elevation position, using a fourth sensor in combination with the first sensor, the second sensor and the third sensor;

The determining the type of the sample container on the sample rack according to the detection results of the first sensor, the second sensor and the third sensor comprises the following steps:

And determining the type of the sample container on the sample rack according to the detection results of the first sensor, the second sensor, the third sensor and the fourth sensor.

3. The method of claim 1, wherein the step of determining the position of the substrate comprises,

Inputting the sample to be detected into an immunoassay detection passageway for detection, and obtaining a first immunoassay result, wherein the method comprises the following steps:

Adding a first reagent into a target reaction cup;

Adding the sample to be detected into the target reaction cup;

sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup;

and detecting the liquid in the target reaction cup, and obtaining a first immune detection result.

4. The method of claim 3, wherein the step of,

After the mixing operation, the incubation operation and the magnetic separation operation are sequentially performed on the liquid in the target reaction cup, the method further comprises the following steps:

Adding a second reagent into the target reaction cup;

And (3) sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup for the second time.

5. The method of claim 4, wherein the step of determining the position of the first electrode is performed,

After the second mixing operation, the incubation operation and the magnetic separation operation are sequentially performed on the liquid in the target reaction cup, the method further comprises the following steps:

Adding a third reagent into the target reaction cup;

and thirdly, sequentially carrying out uniform mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup.

6. The method according to any one of claims 3 to 5, wherein,

The step of sequentially carrying out mixing operation, incubation operation and magnetic separation operation on the liquid in the target reaction cup comprises the following steps:

Sequentially and uniformly mixing the liquid in the target reaction cup;

Placing the target reaction cup in a set temperature environment for incubation operation for a set time length;

And performing magnetic separation operation on the liquid in the target reaction cup.

7. The method of claim 3, wherein the step of,

The detecting the liquid in the target reaction cup and obtaining a first immune detection result comprises the following steps:

Uniformly mixing the liquid in the target reaction cup;

Inputting the liquid in the target reaction cup to an optical detection module so that the optical detection module detects the liquid and a first immune detection result is obtained.

8. The method of claim 1, wherein the step of determining the position of the substrate comprises,

Inputting part of the sample to be detected into an HCT calculation channel, and obtaining a blood cell detection result, wherein the method comprises the following steps:

Adding a diluent into the impedance detection cell;

adding the sample to be detected into the impedance detection pool;

Uniformly mixing the liquid in the impedance detection tank;

and detecting the liquid after the uniform mixing operation in the impedance detection pool, and obtaining a blood cell detection result.

9. The method of claim 8, wherein the step of determining the position of the first electrode is performed,

The step of detecting the liquid after the uniform mixing operation in the impedance detection pool and obtaining a blood cell detection result comprises the following steps:

Detecting the number of red blood cells of the sample to be detected And mean erythrocyte volume/>；

The following formula is adopted to calculate and obtain the hematocritAs a result of blood cell detection:

。

10. the method of claim 8, wherein the step of determining the position of the first electrode is performed,

The calculating to obtain a second immune detection result according to the whole blood detection result and the blood cell detection result comprises the following steps:

calculating to obtain a second immune detection result by adopting the following formula;

；

Wherein, For whole blood detection result,/>Is the blood cell detection result.

11. The method of claim 1, wherein the step of determining the position of the substrate comprises,

Inputting part of the sample to be detected into a colorimetric detection channel, and obtaining a blood cell detection result, wherein the method comprises the following steps:

Adding a hemolytic agent into the impedance detection cell;

adding the sample to be detected into the impedance detection pool;

Uniformly mixing the liquid in the impedance detection tank;

12. The method of claim 11, wherein the step of determining the position of the probe is performed,

Detecting the amount of hemoglobin of the sample to be detected And mean hemoglobin concentration/>；

。

13. the method of claim 11, wherein the step of determining the position of the probe is performed,

；

Wherein, For whole blood detection result,/>Is the blood cell detection result.

14. An immunoassay analyzer, comprising:

A frame;

An impedance tank assembly;

interconnected processor and memory for storing program data, the processor being adapted to execute the program data to implement the method according to any one of claims 1-13.

15. A computer readable storage medium, characterized in that the computer readable storage medium has stored therein program data, which when executed by a processor, is adapted to carry out the method according to any one of claims 1-13.