CN112903401B - Ultrathin section dyeing device - Google Patents

Abstract

The invention discloses an ultrathin section dyeing device which comprises a dyeing box cover, a dyeing box body, a section box cover, a section box body, a clamping groove and an injector. The invention provides a safe, simple and convenient high-flux section dyeing device which can prevent sections from being disordered or falling off and lost in the dyeing and rinsing processes, avoid the section from being polluted by contacting with air, effectively control the flow rate of the double distilled water for rinsing the sections, improve the dyeing efficiency and quality of ultrathin sections, effectively control the dyeing liquid amount and reduce the pollution to the environment.

Description

A kind of ultra-thin slice dyeing device

technical field

The invention relates to a technique for dyeing a sample, in particular to an ultrathin section dyeing device. The slice staining device is used for transmission electron microscope observation and is applied to research in the fields of medical biology and the like.

Background technique

Transmission electron microscopy is applied to observe the ultrastructure of biological samples and plays an important role in medical biology research. The traditional preparation method of transmission electron microscope specimens fixes, dehydrates, dries, embeds and ultra-thinly slices the biological samples, then scoops up the ultra-thin slices onto a grid such as a copper grid or a nickel grid, and then carries out acetic acid double-dipping on the ultra-thin slices. Double staining with uranyl and lead citrate. The traditional staining methods for ultra-thin sections mostly use "drip staining" or "folding staining" and so on. Among them, the steps of the drop dyeing method: drop the dye solution on the wax sheet or parafilm, use tweezers to pick up the carrier net with the ultra-thin section of the biological sample, and the sample surface faces the dye solution drop to cover and float on the drop, and use the utensil Cover the droplet to seal it for staining. After staining, use tweezers to pick up the carrier grid and rinse it with double distilled water. The disadvantages of this method are: 1. Each droplet of the drop dyeing method covers a sample carrier net. When multi-sample slices are required for dyeing, it takes a long time to pick up the carrier net piece by piece and cover it on the droplet, and the dyeing solution is exposed to If the time in the air is long, the dye solution is easy to combine with carbon dioxide in the air to form lead carbonate particles to precipitate and cause pollution, which affects the subsequent section observation; It will cause the dye solution to combine with carbon dioxide in the air to form lead carbonate particles, which will increase the probability of pollution and affect the subsequent section observation; 3. The drip dyeing method can only be dyed and rinsed one by one for a single sample, and sections are prone to appear when the number of samples is large Disorderly, it is difficult to control the consistency of the dyeing time of each sample, and it takes a long time, and the dyeing efficiency is low; Damage the carrier grid or sample sections on the carrier grid during the extraction process. Another commonly used traditional dyeing method is the clip dyeing method. The steps are: vertically clip the carrier net with ultra-thin slices into the paraffin dyeing board, or sandwich the dyeing board made of elastic materials such as silica gel boards and rubber boards or In the section hole on the rubber tube, add the dye solution dropwise to the sample surface of the ultra-thin section grid, use the vessel to cover the staining plate or tube for staining, and use tweezers to pick up the staining plate after dyeing and rinse with double distilled water. The disadvantages of this method are: 1. The pinch dyeing method needs to clamp the grid with ultra-thin slices into the dyeing plate, which is easy to pinch and damage the grid during the operation, causing the wrinkle of the grid to affect the subsequent dyeing and affect the experiment. Results; 2. At present, the commercial dyeing plate is dyed by clamp dyeing method, and the number of samples at one time is mostly about 25 pieces, which is difficult to meet the dyeing requirements of one-time high-throughput samples; 3. A few can dye more than 50 pieces at one time Elastic dyeing boards, the samples need to be tightly clamped together, and cannot be marked independently one by one, which is easy to be confused; 4. During the operation of the clip dyeing method, it is often necessary to open the container covering the dyeing board multiple times, and the dyeing board is clamped out for sample Rinsing, the sample is easy to contact with the air to cause pollution, which affects the subsequent section observation; 5. The clip dyeing method is prone to the situation that the carrier net will fall off during the dyeing and rinsing process due to the instability of the clamp. In addition, if the clip with the carrier net The stained plate is directly rinsed under double-distilled water, and the water flow rate is difficult to control effectively. It is also easy to cause the grid to fall off or the ultra-thin slices of biological samples attached to the grid to be broken or lost, which will affect the subsequent electron microscope observation.

Contents of the invention

Aiming at the defects in the prior art, the invention provides an ultra-thin slice dyeing device.

The technical scheme that the present invention solves the problems of the technologies described above is as follows:

An ultra-thin section dyeing device is composed of a dyeing box cover, a dyeing box body, a section box cover, a section box body, a card slot and a syringe;

1. The structure of each part

The dyeing box cover and the dyeing box body are hollow trapezoids whose top and bottom are rectangular, the bottom area of the dyeing box cover is larger than the top area, the bottom area of the dyeing box body is smaller than the top area, and the bottom of the dyeing box cover can tightly cover the dyeing top of the box.

The upper left corner of the top of the dyeing lid has a dyeing lid liquid injection hole, and the diameter of the hole is 3mm.

The dyeing box body is divided into the upper layer of the dyeing box body, the middle layer of the dyeing box body, and the lower layer of the dyeing box body. The bottom of the lower layer has a length of 74.05mm and a width of 31.05mm.

There is a drain hole on the short side wall at the junction of the long and short side walls of the dyeing box of the dyeing box body, the hole diameter is 3mm, and the connected long side wall is provided with a drain hole plug through a flat silica gel strip, and the hole plug is Solid cylinder with a diameter of 2.99mm and the same height as the thickness of the staining box.

The length of the slice box cover is 74mm, the width is 53mm, and the height is 3mm. At the upper left corner of the top of the cover, the hole diameter is 3mm, the length of the slot on one side of the slicing box cover slot is 74mm, the width is 9mm, and the height is 1mm.

The lid holes of the slicing box are round holes, with 5 rows and 20 columns in total, each column is aligned at the same height, and each column is arranged in parallel at equal intervals. There are 100 holes in total, and the diameter of the holes is 2.5mm.

The slicing box is a T-shaped body with a slicing box liquid injection hole, a slicing hole, a slicing hole label, a slicing box wing, and a bottom hole with a slicing hole at the bottom of the reverse side. The length is 74 mm, the front width is 51 mm, and the back side The width of the bottom is 31mm, and the overall height is 5mm. Among them, the liquid injection hole of the slicing box is located in the upper left corner of the front of the slicing box, and the diameter of the hole is 3mm. The width of the slicing box wing is 10mm, and the height is 0.95mm. After the slicing box is set into the slicing box cover, the overall length is 74mm, the width is 53mm, and the height is 6mm.

The slicing hole is a diamond-shaped hole partially closed at the bottom, with 5 rows and 20 columns in total. Each column is aligned at the same height and each column is arranged in parallel at equal intervals. There are 100 holes in total. The diagonal length of the hole is 3.05mm, and the depth of the hole is 4mm.

The bottom hole of the slicing hole is located at the bottom of the opposite side of the slicing box, and is a round hole, which is a through hole at the bottom of the diamond-shaped slicing hole. There are 5 rows and 20 columns in total, and each column is aligned at the same height, and each column is arranged in parallel at equal intervals, totaling 100 holes , the diameter of the hole is 2.5mm.

The draw-in slot has four side arms in total, which are located 3 mm above the bottom of the two short side walls of the upper layer of the dyeing box body, and extend from the short side walls to the dyeing box body. The length of each side arm slot is 10 mm, and the width is 10mm and a height of 3mm.

The syringe is a universal syringe with a specification of 2ml-10ml and a nipple diameter of 3mm.

The hole of the dyeing box cover has a cross cut, and the cut is sealed under no pressure.

2. The connection relationship of each component

The slicing box cover slot is set outside the wing of the slicing box body, and the slicing box cover and the slicing box body form a tightly connected whole, which is tightly fitted in the card slot; by vertically pressing the upper layer of the dyeing box body, the dyeing box The middle layer of the body is folded down to form the upper layer of the dyeing box body, the middle layer of the dyeing box body, and the lower layer of the dyeing box body from outside to inside, so that the bottom of the slicing box fits flat on the bottom of the lower layer of the dyeing box, thereby realizing a T-shaped slicing box The lower part of the body is tightly wrapped in the lower layer of the dyeing box body; when the dyeing box body is folded, the liquid injection hole of the dyeing box cover, the liquid injection hole of the section box cover, and the liquid injection hole of the section box body are connected vertically and parallelly; before dyeing, the dyeing box cover is set Tightly connect the dyeing box body, plug the drain hole plug into the drain hole, and form a closed environment in the whole dyeing box; use a syringe to suck the air in the dyeing box through the liquid injection hole or drain hole of the dyeing box cover; dyeing When using a syringe, inject the dye solution into the slice box through the liquid injection hole of the dyeing box cover, the liquid injection hole of the slice box cover, and the liquid injection hole of the slice box body in sequence for dyeing; Stretch the upper layer and the middle layer of the dyeing box upwards to restore the original position, pull out the plug of the drain hole to open the drain hole, use a syringe to suck out the dye solution through the drain hole and recover it, and then drain the double distilled water for rinsing the slices.

The above-mentioned dyeing box cover, dyeing box body and card slot are made of transparent silica gel with good elasticity, the sectioning box cover is made of transparent plastic with stable acid and alkali resistance, and the sectioning box body is made of plastic with stable acid and alkali resistance.

The above slicing hole labels use Arabic numerals or Greek letters and English letters respectively, and correspond to the rows and columns of the slicing holes in order.

The bottom of the lower layer of the dyeing box body can be better adhered to a smooth plane and is not easy to slide, and an external force is applied to the side of the bottom of the lower layer of the dyeing box body to lift up, so as to realize the separation of the lower layer of the color box body from the originally firmly adhered smooth plane .

Advantage or beneficial effect of the present invention:

1. The present invention can dye up to 100 samples at a time by utilizing the slicing box with the slicing box lid hole, slicing hole, slicing hole label, and slicing hole bottom hole to dye ultra-thin slices, which satisfies the It needs to be stained with multiple samples at the same time, and each slice is placed in a slice hole separately. The cover of the slice box can prevent the slices from falling out. The corresponding slice hole label can help identify the samples in each well. During staining and In the process of rinsing, there will be no confusion in the position of the slices and loss of loss, and the staining efficiency will be significantly improved.

2. The present invention utilizes the slicing box with slicing box lid hole, slicing hole, bottom hole of slicing hole to carry out the dyeing of ultra-thin section, and three kinds of holes are vertically connected, and dyeing solution and double distilled water can pass through the hole that slices in the hole. The ultra-thin sections are completely submerged. During the dyeing and rinsing process, only the dye solution and double-distilled water need to be injected and drained, and there is no need to use tweezers to clamp the grid, which effectively avoids the easy damage of the sections caused by multiple clamping.

3. In the present invention, the dyeing box cover and the dyeing box body made of transparent silica gel are sealed for dyeing. After the air in the dyeing box is sucked with a syringe before dyeing, the separation box is not needed in the subsequent dyeing and rinsing process of ultra-thin sections. The cover and the box body only need to dye and inject double distilled water through the liquid injection hole on the dyeing box cover, and recover the dye solution and discharge double distilled water through the liquid drainage hole on the dyeing box body, effectively avoiding the separation of slices and Air contact greatly reduces the probability of pollution caused by the combination of dye liquor and carbon dioxide in the air to form precipitation.

4. The present invention uses a card slot made of silica gel with better elasticity, which can firmly fit the slicing box containing the ultra-thin slices on the upper layer of the dyeing box body, and press and fold the dyeing box when dyeing the ultra-thin slices body, so that the bottom of the slicing box fits flat on the bottom of the staining box, use a syringe to inject about 2.5ml of dye solution into the slicing box through the liquid injection hole of the staining box cover and the liquid injection hole of the slicing box cover from the outside to the inside In the liquid injection hole, the hydrophobicity of the bottom surface of the dyeing box will make the dyeing solution enter the slicing hole through the bottom hole of the slicing hole, and then fill all the slicing holes of the entire slicing box, which can effectively avoid possible damage caused by directly injecting the dyeing solution against the slice. Washing damage; after dyeing, lift the dyeing box body to restore it, so that the section box can be suspended in the dyeing box body, and when the double distilled water is injected through the liquid injection hole of the dyeing box cover for rinsing, the double distilled water can flow through the dyeing box cover and inject Liquid hole, slicing box cover hole, slicing hole, and slicing hole bottom hole realize gentle rinsing of ultra-thin slices in the slicing hole, effectively avoiding damage to slices caused by direct rinsing.

5. The present invention uses a dyeing box made of transparent silica gel for dyeing, which is easy to observe the sample situation through the box cover; and the bottom of the section box is tightly wrapped in the lower layer of the dyeing box, and the volume is fixed, which can better control the amount of dyeing solution and avoid The waste of dyeing solution is also conducive to reducing the pollution of heavy metal dyeing solution to the environment.

Description of drawings

Fig. 1 is a schematic diagram of the structure of the slice staining device of the present invention.

In the figure: dyeing box cover 1, dyeing box body 2, slice box cover 3, slice box body 4, card slot 5, syringe 6, dyeing box cover liquid injection hole 7, dyeing box upper layer 8, dyeing box middle layer 9, Dyeing box lower layer 10, drain hole 11, drain hole plug 12, slice box cover liquid injection hole 13, slice box cover hole 14, slice box cover slot 15, slice box body liquid injection hole 16, slice hole 17, Slicing hole label 18, slicing box body wing 19, slicing hole bottom hole 20, cross cut 21.

Fig. 2 is a transmission electron microscope image obtained after staining with a section staining device according to an embodiment of the present invention.

Detailed ways

The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

An ultra-thin section dyeing device of the present invention is shown in Figure 1, and an ultra-thin section dyeing device is composed of a dyeing box cover 1, a dyeing box body 2, a section box cover 3, a section box body 4, a card slot 5 and a syringe 6 .

1. The structure of each part

The dyeing box cover 1 and the dyeing box body 2 are hollow trapezoids whose top and bottom are rectangular, the bottom area of the dyeing box cover 1 is greater than the top area, and the bottom area of the dyeing box body 2 is smaller than the top area, and the dyeing box cover 1 The bottom can tightly cover the top of the staining box body 2.

The upper left corner of the top of the dyeing lid 1 has a dyeing lid liquid injection hole 7, and the diameter of the hole is 3mm.

Described dyeing box body 2 is divided into dyeing box body upper layer 8, dyeing box body middle layer 9, dyeing box body lower layer 10, the height of dyeing box body upper layer 8 is 25mm, and the height of dyeing box body middle layer 9 and dyeing box body lower floor 10 is identical , is 3mm, and the bottom length of dyeing box lower layer 10 is 74.05mm, and width is 31.05mm.

The dyeing box lower layer 10 of the dyeing box 2 has a drain hole 11 on the short side wall at the junction of the long and short side walls, and the diameter of the hole is 3 mm, and the connected long side wall is provided with a drain hole plug 12 through a flat silica gel strip. , the hole plug is a solid cylinder with a diameter of 2.99mm and the same height as the thickness of the lower layer 10 of the dyeing box body.

The length of described slicing lid 3 is 74mm, and width is 53mm, high 3mm, is the hollow cuboid that has slicing lid liquid injection hole 13, slicing lid hole 14, slicing lid groove 15, and slicing lid injection Hole 13 is positioned at the top left corner of slice box cover 3, and hole diameter is 3mm, and the length in the single side groove of slice box cover draw-in groove 15 is 74mm, and width is 9mm, and height is 1mm.

The slicing box lid holes 14 are round holes, with 5 rows and 20 columns in total, each column is aligned at the same height, and each column is arranged in parallel at equal intervals. There are 100 holes in total, and the diameter of the holes is 2.5mm.

The slicing box body 4 is a T-shaped body with a slicing box body liquid injection hole 16, a slicing hole 17, a slicing hole label 18, a slicing box body wing 19 and a bottom hole 20 on the reverse side, and the length is 74 mm. The width of the front is 51mm, the width of the bottom of the back is 31mm, and the overall height is 5mm. Among them, the liquid injection hole 16 of the slicing box is located at the upper left corner of the front of the slicing box 4, the hole diameter is 3mm, and the label 18 of the slicing hole is respectively located at the slicing box 4. The front left side and the lower side of the front side, the width of the unilateral slicing box body wing 19 is 10mm, and the height is 0.95mm.

The slicing hole 17 is a diamond-shaped hole partially closed at the bottom, with 5 rows and 20 columns in total, each column is aligned at the same height, and each column is arranged in parallel at equal intervals. There are 100 holes in total. The diagonal length of the hole is 3.05mm, and the depth of the hole is is 4mm.

The bottom hole 20 of the slicing hole is located at the bottom of the opposite side of the slicing box body 4, and is a round hole, which is a through hole at the bottom of the rhombus-shaped slicing hole 17. There are 5 rows and 20 columns in total, and each column is aligned at the same height, and the columns are arranged in parallel at equal intervals. There are 100 holes in total, and the diameter of the holes is 2.5mm.

Described draw-in groove 5 has four side arms altogether, is positioned at dyeing box body top layer 8 two short side wall bottoms up 3mm place, stretches in the air from short side wall to dyeing box body 2, and the length in each side arm groove is 10mm, the width is 10mm, and the height is 3mm.

The syringe 6 is a universal syringe with a specification of 2ml to 10ml and a nipple diameter of 3mm;

The dyeing box cover hole 7 has a cross cut 21, and the cut is sealed under no pressure.

The connection relationship of each component

The described slicing box cover slot 15 is set on the outside of the slicing box body wing 19, the slicing box cover 3 and the slicing box body 4 form a tightly connected whole, which is tightly fitted in the slot 5; by pressing down vertically The upper layer 8 of the dyeing box and the middle layer 9 of the dyeing box are folded down to form the upper layer 8 of the dyeing box, the middle layer 9 of the dyeing box, and the lower layer 10 of the dyeing box from outside to inside, so that the bottom of the section box fits flat Dye the bottom of the lower layer 10 of the box body, and then realize that the bottom of the T-shaped section box body 4 is tightly wrapped in the lower layer 10 of the dyeing box body; 1. The liquid injection hole 16 of the slicing box body realizes vertical and parallel communication; before dyeing, the dyeing box cover 1 is set on the dyeing box body 2 and tightly connected, and the drain hole plug 12 is inserted into the drain hole 11, and the entire dyeing box forms an airtight Environment; use syringe 6 to suck the air in the dyeing box through the dyeing box cover liquid injection hole 7 or liquid drainage hole 11; Hole 13 and injection hole 16 of the slicing box body are injected into the slicing box for dyeing; after dyeing, the upper layer 8 of the dyeing box body and the middle layer 9 of the dyeing box body are stretched upwards and restored to their original positions by pulling the upper layer 8 of the dyeing box body, and the liquid is drained out. The hole plug 12 opens the drain hole 11, and the syringe 6 is used to suck out and recover the dye solution through the drain hole 11, and then discharge the double distilled water for rinsing the slices.

The above-mentioned dyeing box cover 1, dyeing box body 2, and draw-in slot 5 are made of transparent silica gel with good elasticity; the sectioning box cover 3 is made of transparent plastic with stable acid and alkali resistance; plastic

The above slicing hole labels 18 respectively use Arabic numerals or Greek letters and English letters, correspondingly marking the rows and columns of the slicing holes 17 in order.

The bottom of the lower layer 10 of the dyeing box body can be better adhered to a smooth plane and is not easy to slide. By applying an external force to the side of the bottom of the lower layer of the dyeing box body 10, it is lifted up to realize the firm adhesion of the lower layer 10 of the color box body to the original smooth plane separation.

The steps of using the ultra-thin section dyeing device of the present invention to dye the sample are:

1. Preparation: put the ultra-thin slices to be stained into the slice hole 17 of the slice box 4 one by one in order, and put the slice box 4 into the slice box cover slot 15 through the slice box wing 19 in parallel, so that the slice box The cover 3 is tightly fitted outside the slicing box body 4 to form the whole slicing box, and then tightly fitted in the card slot 5, and the upper layer 8 of the dyeing box body 2 is pressed vertically downward to realize the folding down of the middle layer 9 of the dyeing box body , forming the upper layer 8 of the dyeing box body, the middle layer 9 of the dyeing box body, and the lower layer 10 of the dyeing box body from the outside to the inside, so that the bottom of the section box tightly fitted in the card slot 5 fits and lays flat on the dyeing box body 2. The bottom of the lower layer 10 of the box body, and then realize that the bottom of the T-shaped section box body 4 is tightly wrapped in the lower layer of the dyeing box body 10; The liquid injection hole 16 of the slicing box realizes vertical and parallel communication.

2. Put the dyeing box cover 1 on the dyeing box body 2 to realize tight connection, put the drain hole plug 12 into the drain hole 11, and form a closed environment in the whole dyeing box.

3. Use the syringe 6 to suck out the air in the airtight dyeing box from the liquid injection hole 7 of the dyeing box cover.

4. Use syringe 6 to inject about 2.5ml of uranyl acetate dye solution from the outside to the inside through the liquid injection hole of the dyeing box cover and the liquid injection hole of the section box cover into the liquid injection hole of the section box body. The dyeing solution will enter the slicing hole through the bottom hole of the slicing hole, and then fill all the slicing holes of the whole slicing box. Observe the height of the dyeing solution through the transparent staining box cover until the level of the dyeing solution is slightly higher than or flush with the hole of the slicing box cover. , ensure that the dye solution can completely submerge the ultra-thin slices in the slice hole, pull out the syringe, and let it stand for 15min to 25min for staining.

5. Lift the upper layer of the dyeing box, and stretch the folded down middle layer of the dyeing box upwards to recover, so that the upper layer of the dyeing box, the middle layer of the dyeing box, and the lower layer of the dyeing box are restored to their original positions, and the section box is suspended in the air. In the dyeing box, pull out the drain hole plug to open the drain hole, and use a syringe to suck out the dye solution through the drain hole for recovery.

6. Plug the drain hole plug into the drain hole to close it, use a syringe to slowly inject double distilled water into the staining box along the liquid injection hole of the staining box cover, observe the liquid level in the staining box, and ensure that the double distilled water will cover the slices The box is completely submerged, and the staining box can be gently shaken to further improve the rinsing effect. After rinsing for 1-5 minutes, use a syringe to drain the double-distilled water for rinsing the slices. This step is repeated at least 3 times.

7. Gently shake the dyeing box to promote the outflow of residual rinse solution, and use a syringe to suck it all out.

8. Vertically press down the upper layer 8 of the dyeing box body 2, and then set the upper layer 8 of the dyeing box body, the middle layer 9 of the dyeing box body, and the lower layer 10 of the dyeing box body in order from outside to inside, so that they are tightly fitted in the card slot 5 The bottom of the inner slicing box fits flatly on the bottom of the lower layer 10 of the dyeing box, so that the lower part of the T-shaped slicing box 4 is tightly wrapped in the lower layer 10 of the dyeing box.

9. Put the drain hole plug 12 into the drain hole 11 to form a sealed environment in the entire dyeing box.

10. Use the syringe 6 to suck out the air in the airtight dyeing box from the liquid injection hole 7 of the dyeing box cover

11. Use syringe 6 to inject about 2.5ml of lead citrate dye solution from outside to inside through the liquid injection hole of the dyeing box cover and the liquid injection hole of the section box cover into the liquid injection hole of the section box body, and observe the staining through the transparent dyeing box cover. Make sure that the dye solution can completely submerge the ultra-thin slices in the section hole until the level of the dye solution is slightly higher than or flush with the hole of the slicing box cover. Pull out the syringe and let it stand for 5 to 10 minutes for staining.

12. Lift the upper layer of the dyeing box body, and stretch the folded down middle layer of the dyeing box body upward to recover, so that the upper layer of the dyeing box body, the middle layer of the dyeing box body, and the lower layer of the dyeing box body are restored to their original positions, and the section box is suspended in the air. In the dyeing box, pull out the drain hole plug to open the drain hole, and use a syringe to suck out the dye solution through the drain hole for recovery.

13. Insert the drain hole plug into the drain hole to close it, use a syringe to slowly inject double distilled water into the staining box along the liquid injection hole of the staining box cover, observe the liquid level in the staining box, and ensure that the double distilled water will cover the slices. The box is completely submerged, and the staining box can be gently shaken to further improve the rinsing effect. After rinsing for 1-5 minutes, use a syringe to drain the double-distilled water for rinsing the slices. This step is repeated at least 3 times.

14. Gently shake the dyeing box to promote the outflow of residual rinse solution, and use a syringe to suck it all out.

15. Open the staining box and slicing box one by one, use tweezers to take out the ultra-thin section clips one by one from the section hole, use dust-free filter paper to dry up the rinse solution that may remain on the section, and then put the section into the clean room in a glass petri dish. Dust filter paper, placed in a 37 ℃ oven to dry.

16. Conduct transmission electron microscope observation and image collection of the dyed sample, as shown in Figure 2.

In a word, the above descriptions are only preferred embodiments of the present invention, and all equivalent changes and modifications made according to the scope of the patent application of the present invention shall fall within the scope of the patent of the present invention.

Claims (4)

1. An ultra-thin section dyeing device, characterized in that: the device consists of a dyeing box cover (1), a dyeing box body (2), a section box cover (3), a section box body (4), a card slot (5) and The syringe (6) consists of; Combination of components The dyeing box cover (1) and the dyeing box body (2) are hollow trapezoids whose top and bottom are rectangular, the bottom area of the dyeing box cover (1) is larger than the top area, and the bottom area of the dyeing box body (2) is smaller than Top area, the bottom of the dyeing box cover (1) can tightly cover the top of the dyeing box body (2); The top left corner of the dyeing box cover (1) has a dyeing box cover liquid injection hole (7), and the diameter of the hole is 3 mm; The dyeing box (2) is divided into the upper layer of the dyeing box (8), the middle layer of the dyeing box (9), and the lower layer of the dyeing box (10). The height of the upper layer of the dyeing box (8) is 25 mm, and the middle layer of the dyeing box (9) The height of the lower layer of the dyeing box body (10) is the same as 3mm, the length of the bottom of the lower layer of the dyeing box body (10) is 74.05mm, and the width is 31.05mm; The lower layer of the dyeing box (10) of the dyeing box (2) has a drain hole (11) on the short side wall at the junction of the long and short side walls. The diameter of the hole is 3mm. There is a drain hole plug (12), the hole plug is a solid cylinder with a diameter of 2.99 mm, and the height is the same as the thickness of the lower layer of the dyeing box body (10); The length of the slicing box cover (3) is 74mm, the width is 53mm, and the height is 3mm. Hollow cuboid, the liquid injection hole (13) of the slicing box cover (3) is located at the upper left corner of the top of the slicing box cover (3), the hole diameter is 3 mm, the length of the single side of the slicing box cover slot (15) is 74 mm, and the width is 9 mm. The height is 1mm; The slicing box cover holes (14) are round holes, with 5 rows and 20 columns in total, each column is aligned at the same height, and each column is arranged in parallel at equal intervals, totaling 100 holes, and the diameter of the holes is 2.5mm; The slicing box (4) is equipped with a slicing box liquid injection hole (16), a slicing hole (17), a slicing hole label (18), a slicing box body wing (19) and a bottom hole with a slicing hole at the bottom of the reverse side The T-shaped body of (20) has a length of 74 mm, a front width of 51 mm, a back bottom width of 31 mm, and an overall height of 5 mm. The liquid injection hole (16) of the slicing box is located at the upper left corner of the front of the slicing box (4) , the diameter of the hole is 3mm, the slice hole label (18) is located on the front left side and the lower side of the slice box (4), the width of the single side slice box wing (19) is 10mm, the height is 0.95mm, the slice box (4) After being put into the slice box cover (3), the overall length is 74mm, the width is 53mm, and the height is 6mm; The slicing holes (17) are diamond-shaped holes partially closed at the bottom, with 5 rows and 20 columns in total. Each column is aligned at the same height and each column is arranged in parallel at equal intervals. There are 100 holes in total. The diagonal length of the holes is 3.05mm. The depth is 4mm; The bottom hole (20) of the slice hole (20) is located at the bottom of the reverse side of the slice box body (4). The columns are arranged in parallel at equal intervals, with a total of 100 holes, and the diameter of the holes is 2.5mm; The card slot (5) has a total of four side arms, which are located 3 mm above the bottom of the two short side walls of the upper layer of the dyeing box body (8), extending from the short side walls to the inside of the dyeing box body (2), each The length of the side arm slot is 10mm, the width is 10mm, and the height is 3mm; The syringe (6) is a universal syringe with a specification of 2ml to 10ml and a nipple diameter of 3mm; The liquid injection hole (7) of the dyeing box cover has a cross cut (21), and the cut is sealed under no pressure; The connection relationship of each component The slicing box cover slot (15) is set on the outside of the slicing box body wing (19), and the slicing box cover (3) and the slicing box body (4) form a tightly connected whole, which is tightly fitted in the slot (5 ); by vertically pressing down the upper layer of the dyeing box body (8) and the middle layer of the dyeing box body (9) to fold down, the upper layer of the dyeing box body (8), the middle layer of the dyeing box body (9), and the lower layer of the dyeing box body (10 ) from the outside to the inside, so that the bottom of the slice box fits flat on the bottom of the lower layer of the dyeing box (10), and then the lower part of the T-shaped slice box (4) is tightly wrapped in the lower layer of the dyeing box (10) ; When the dyeing box body (2) is folded, the liquid injection hole (7) of the staining box cover, the liquid injection hole (13) of the section box body, and the liquid injection hole (16) of the section box body are connected vertically and parallelly; before dyeing, the staining box cover (1) The set is tightly connected to the dyeing box body (2), and the drain hole plug (12) is inserted into the drain hole (11), forming a closed environment in the entire dyeing box; use the syringe (6) to pass through the dyeing box cover The liquid injection hole (7) or the liquid drainage hole (11) sucks the air in the dyeing box; when dyeing, use the syringe (6) to pass the dyeing solution through the liquid injection hole (7) of the staining box cover and the liquid injection hole of the slice box cover in sequence (13), the liquid injection hole (16) of the slicing box body is injected into the slicing box for dyeing; after staining, the upper layer of the dyeing box body (8) and the middle layer of the dyeing box body (9) are drawn by pulling the upper layer of the dyeing box body Stretch upwards to return to the original position, pull out the drain hole plug (12) to open the drain hole (11), use the syringe (6) to suck out the dye solution through the drain hole (11) and recover, and then double-evaporate the rinsed slices. water to discharge. 2. A dyeing device for ultra-thin slices according to claim 1, characterized in that: the dyeing box cover (1), the dyeing box body (2), and the slot (5) are made of transparent silica gel with good elasticity The slicing box cover (3) is made of transparent plastic with stable acid and alkali resistance, and the slicing box body (4) is made of plastic with stable acid and alkali resistance. 3 . The ultra-thin section staining device according to claim 1 , characterized in that: the section hole labels ( 18 ) respectively use Arabic numerals to correspond to the rows and columns of the section holes ( 17 ) in order. 4 . 4. A dyeing device for ultra-thin slices according to claim 1, characterized in that: the bottom of the lower layer (10) of the dyeing box body can be better adhered to a smooth plane and is not easy to slide, and the bottom of the dyeing box body The side of the bottom of the lower layer (10) is lifted upwards by applying an external force, so as to separate the lower layer (10) of the color box body from the originally firmly adhered smooth plane.

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