CN1128103A - Powdered milk containing active bifidobacterium and its producing method - Google Patents
Powdered milk containing active bifidobacterium and its producing method Download PDFInfo
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- CN1128103A CN1128103A CN 95111712 CN95111712A CN1128103A CN 1128103 A CN1128103 A CN 1128103A CN 95111712 CN95111712 CN 95111712 CN 95111712 A CN95111712 A CN 95111712A CN 1128103 A CN1128103 A CN 1128103A
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- bifidobacterium
- milk powder
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- bacterium
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- 241000186000 Bifidobacterium Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 17
- 235000008476 powdered milk Nutrition 0.000 title abstract 2
- 239000000843 powder Substances 0.000 claims abstract description 45
- 241000894006 Bacteria Species 0.000 claims abstract description 32
- 238000007710 freezing Methods 0.000 claims abstract description 7
- 230000008014 freezing Effects 0.000 claims abstract description 7
- 238000004108 freeze drying Methods 0.000 claims abstract description 5
- 230000008569 process Effects 0.000 claims abstract description 5
- 235000013336 milk Nutrition 0.000 claims description 30
- 239000008267 milk Substances 0.000 claims description 30
- 210000004080 milk Anatomy 0.000 claims description 30
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 239000003223 protective agent Substances 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
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- 230000004083 survival effect Effects 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 4
- 238000007711 solidification Methods 0.000 claims description 4
- 230000008023 solidification Effects 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 239000002778 food additive Substances 0.000 claims description 3
- 235000013373 food additive Nutrition 0.000 claims description 3
- 238000000859 sublimation Methods 0.000 claims description 3
- 230000008022 sublimation Effects 0.000 claims description 3
- 108010082495 Dietary Plant Proteins Proteins 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- 239000006071 cream Substances 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 235000020183 skimmed milk Nutrition 0.000 claims description 2
- 230000005059 dormancy Effects 0.000 claims 1
- 238000011081 inoculation Methods 0.000 claims 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 4
- 230000036039 immunity Effects 0.000 abstract description 2
- 239000004310 lactic acid Substances 0.000 abstract description 2
- 235000014655 lactic acid Nutrition 0.000 abstract description 2
- 230000007423 decrease Effects 0.000 abstract 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 230000036541 health Effects 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000004821 distillation Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000004051 gastric juice Anatomy 0.000 description 3
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- 230000004060 metabolic process Effects 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000186012 Bifidobacterium breve Species 0.000 description 2
- 241001608472 Bifidobacterium longum Species 0.000 description 2
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
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- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000186018 Bifidobacterium adolescentis Species 0.000 description 1
- 241000186015 Bifidobacterium longum subsp. infantis Species 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
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- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 229940004120 bifidobacterium infantis Drugs 0.000 description 1
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- 230000008859 change Effects 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
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- 239000010985 leather Substances 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
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- 230000002265 prevention Effects 0.000 description 1
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000192 social effect Effects 0.000 description 1
- -1 spiramvcin Chemical compound 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
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- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Bifidobacterium as one kind of benign lactic acid bacteria inside enteron can raise human immunity and its amount decreases owing to senility and other causes. The powdered milk powder of the present invention contains sufficient freeze-dried Bifidobacterium powder and is prepared through the technological process designed based on the growth conditions of Bifidobacterium. By means of the control of freezing and lyophilization conditions, the live bacteria are made into dormant state and vacuum packed. The said techn process is suitable for mass prodn.
Description
The present invention relates to microorganism culture process and dairy products processing technology.
According to Caucasia, Bulgarian certain areas long lived elder are investigated, the reason that confirms their longevity is that the more lactic acid bacteria than ordinary person is arranged in the enteron aisle.For this reason, the problem of food additives is all made in countries in the world how with these viable bacterias in research from the fifties end beginning.So that under the situation that does not change the flavour of food products quality, improve the health care of food function, keep the breeding balance of enteron aisle flora, play diseases prevention, the effect of curing the disease, safeguard human health longevity.
Bifidobacterium is a kind of leather Lan Shi positive bacteria that colonizes in the enteron aisle, produces organic enzyme in metabolic process, and the effect that suppresses the growth of enteron aisle saprophytic bacteria is arranged.Thereby play anticancerly, strong liver is anti-ly treated constipation, suffers from diarrhoea, and reduces cholesterol, improves vitamin metabolism, improves effects such as immunity function, and health is had multinomial beneficial functional.The Bifidobacterium of survival in human body intestinal canal is a kind of anaerobic bacteria, and its oxygen-resistant ability is different and different with mushroom.For example, bifidobacterium infantis (Bifiaobacteriuminfartrs), bifidobacterium breve (B.breve), bifidobacterium longum (B.Longum) has certain oxytolerant power, and being grown under the low oxygen partial pressure of bifidobacterium adolescentis (B.acblescentis) promptly is suppressed.In addition, the people takes a large amount of antibiolics because of ill need usually.As: erythromycin, spiramvcin, chloramphenicol, they have very big lethality to Bifidobacterium.So, with manual method this class viable bacteria is made food, beverage, constantly human body is replenished, adds, then have very big health nutrient and be worth and social effect.
Bifidobacterium growth condition harshness, its growth temperature are at 37～43 ℃, and growth acidity pH value is 6.5～7.0.Make oral active Bifidobacterium can field planting in enteron aisle, numerous planting, must accomplish 2 points: the one, by stomach the time, do not killed (prove according to the study the normal Bifidobacterium of metabolism has to understand 90% or more be killed) by hydrochloric acid in gastric juice by hydrochloric acid in gastric juice; The 2nd, certain Bifidobacterium amount be arranged, its nutrition health-care functions of competence exertion (is generally 10
5-10
6Individual/more than the milliliter).The Bifidobacterium oral liquid of present city pin, Bifidobacterium food, most because of cultivating condition restriction such as processing, preservation, the survival bacterium is measured not high problem ubiquity.Final products are semi-fluid or liquid in addition, so shortcomings such as transportation inconvenience, viable bacteria storage life weak point are arranged again, do not reach desirable health care purpose.91 years Xinjiang Ya Xi health products milk sheet factories once proposed to make with eight kinds of bacterium such as France, Japan, the Soviet Union method (patent publication No.: CN1059993A) of activiated lactacidase milk tablet, because relate to that bacterial classification is many, manufacturing process and plant machinery degree be low, be not suitable for suitability for industrialized production, and it is also big to the survival rate influence of bacterium to add tablet forming technique.
The objective of the invention is to adopt good bifidobacterium species; according to these bacteria growing condition needs; under anaerobic environment, cultivate breed a certain amount of after; add nourishment protecting agent; and viable bacteria is converted into the live body freeze-dried powder of resting state with freezing, distillation, drying means; mix in proportion with milk powder, packing is made and is contained active Bifidobacterium milk powder again.Take after mixing it with water with warm water when edible, time and term harmonization that the viable bacteria hypopus in the freeze-dried powder is just in time recovered with viable bacteria to time of enteron aisle by stomach, thus reach the purpose that most viable bacterias can field planting in enteron aisle.
Manufacture method of the present invention mainly comprises six technical process and relevant prescription:
One, bacterial classification: the good edible Bifidobacterium of identifying through Institute of Microorganism, Academia Sinica.
Two, medium preparation: 1, the 1200ml fresh milk is carried out degreasing (being about to the milk liquid that fresh milk obtains after centrifugal filtration), moist heat sterilization under 15KPa pressure, the cooling back is standby; 2, in defatted milk, add 1% sterilized glucose, 3% sterilized yellow seriflux, and use NH
3H
2O regulates PH=6.8, and it is standby that cooling is placed on 0-7 ℃ of refrigerator cold-storage.
Three, bacterial classification is handled: main technique is bacterial classification to be placed on carry out inoculated and cultured in the culture medium, till three expansion cultivations reach three-class strain always.Concrete steps are as follows: the test tube that 1, strain inclined plane is inserted fluid nutrient medium is cultivated, and regulates PH=6.8, and 42 ℃ of temperature about 3 hours, promptly get second class inoculum.2, second class inoculum is inserted in the 500ml nutrient solution, transfer 37 ℃ of PH=6.8, temperature, after 16 hours, promptly get three-class strain, it is standby to store in refrigerator.
Four, thalline is cultivated: 1, create anaerobic environment: putting into concentration by the amount of volume 1% in the drier is 15%NaOH and 1 gram pyrogallic acid, and lighted candle produces CO with oxygen
2, form anaerobic environment.2, cultivate the thalline group: the 2000ml nutrient solution is put into the small-sized fermentation jar, and insert three grades of seeds of 5% amount, regulate PH=6.8,37 ℃ of temperature were cultivated 16 hours.3, counting: dilution suitable multiple, microscopic count reach 10
10Individual/milliliter then stops to cultivate, less than 10
10Individual/milliliter, then continue to prolong to cultivate, up to reaching 10
10Till individual/milliliter.
Five, add nourishment protecting agent, freezing, distillation, drying:
1, preparation protective agent: nourishment protecting agent is the hotbed that viable bacteria depends on for existence, and the emulsion that it is made up of glucose, skimmed milk power, cream belongs to protective agent.
2, with three-class strain with after protective agent fully mixes, put into freeze dryer and treat that freeze-drying handles.
3, freezingly contain protectant flora, freezing condition is as follows: 1. solidification point is-30 ℃～-20 ℃; 2. the time by normal temperature arrival solidification point is 60～150 minutes; (Best Times 120 minutes) 3. freeze-drying vacuum 1.33～13.2Pa.
4, distillation is freezing.Sublimation condition: temperature of charge-20 ℃, chilling temperature-40 ℃ after 36 hours, can obtain water content and be 0.13～1.3% white Bifidobacterium freeze-dried powder.
Six, sieve, mix, pack:
1, freeze-dried powder is pulverized, 60 orders-80 order sieves.
2, freeze-dried powder is mixed in proportion with milk powder, the every gram of the mixed milk powder of proportion requirement contains the viable bacteria amount and is no less than 2,000,000.
3, will contain bacterium milk powder and carry out packing by pouch, every pouch can contain the bacterium spore more than 4,000 ten thousand.
4, vacuum packaging contains bacterium milk powder.
The viable bacteria milk powder of being produced in this way that contains has the following advantages: fully cultivate in conjunction with Bifidobacterium life condition and environment, make every gram milk powder obtain nearly 2,000,000 viable bacterias; Utilize Bifidobacterium in cryogenic freezing lyophilization process, to form the characteristics and spore recovery 3-5 hour the time difference of palpus of resting state live body, guarantee to contain and in human stomach, avoid the injury of hydrochloric acid in gastric juice after bacterium milk powder is taken after mixing it with water, make it have more viable bacteria to enter the enteron aisle survival and reproduction it; It is high (if still can guarantee 10 with 1: 7 water Instant Drinks that this milk powder not only contains the viable bacteria amount
5～10
6Individual/milliliter), and easily preserve (more than 1 year) because of vacuum packaging, manufacturing process is simple, and cost is low, and convenient transportation is of high nutritive value, and Instant Drinks are easy to use.
Accompanying drawing: for containing Bifidobacterium milk powder manufacturing process flow diagram, wherein C is that glucose, N are yellow seriflux.
Other embodiment of the present invention:
1, except that milk powder, can also select edible pulvis such as bean powder, ground rice, flour, corn flour, powdered glucose, pollen, medicinal powder, animal/vegetable protein powder for use with the mixture of Bifidobacterium freeze-dried powder.
2, the also available enteric material of Bifidobacterium freeze-dried powder that obtains of the present invention is directly made enteric solubility powder or particle as food additives or directly edible.
3, two sloping bacillus milk powder and Bifidobacterium freeze-dried powder preparation thereof packing can be packed, box-packed, canned, tinned, bottled, or packs with capsule.
Claims (7)
1, contains active Bifidobacterium milk powder and it is characterized in that it mixes the flour of forming by containing the active Bifidobacterium freeze-dried powder in proportion with milk powder.
2, the viable bacteria milk powder that contains according to claim 1 is characterized in that the freeze-dried powder that contains active Bifidobacterium can mix composition in proportion with edible pulvis such as bean powder, ground rice, powdered glucose, pollen, medicinal powder, animal/vegetable protein powder.
3, the manufacture method that contains active Bifidobacterium milk powder comprises inoculation, the cultivation of Bifidobacterium; forming freeze-dried powder mixes with milk powder; technical process such as packing; the inoculated and cultured that it is characterized in that active Bifidobacterium is to form in special culture medium and anaerobic environment; and survive in special nourishment protecting agent, its freezing and sublimation, drying condition are according to designed processing of specified conditions such as flora survival, dormancy, recoveries.
4, the bacterium milk powder manufacture method that contains according to claim 3 is characterized in that the emulsion that special nourishment protecting agent is made up of glucose, skimmed milk power, cream.
5, the bacterium milk powder manufacture method that contains according to claim 3 is characterized in that the lyophilisation condition of designed processing is meant that 1. solidification point is-30 ℃～-20 ℃; 2. the time by normal temperature arrival solidification point is 60～150 minutes; 3. freeze-drying vacuum 1.33～13.2Pa; 4. sublimation condition: temperature of charge-20 ℃, condenser temperature-40 ℃.
6, the bacterium milk powder manufacture method that contains according to claim 3, it can be packed, box-packed, canned that the packing that it is characterized in that containing viable bacteria milk powder forms, or packs with capsule.
7,, it is characterized in that the also available enteric material of the Bifidobacterium freeze-dried powder that obtains directly makes enteric solubility granular food additive or directly edible according to claim 3, the 5 described bacterium milk powder manufacture methods that contain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95111712 CN1128103A (en) | 1995-07-31 | 1995-07-31 | Powdered milk containing active bifidobacterium and its producing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95111712 CN1128103A (en) | 1995-07-31 | 1995-07-31 | Powdered milk containing active bifidobacterium and its producing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1128103A true CN1128103A (en) | 1996-08-07 |
Family
ID=5078970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95111712 Pending CN1128103A (en) | 1995-07-31 | 1995-07-31 | Powdered milk containing active bifidobacterium and its producing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1128103A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305383C (en) * | 2003-11-03 | 2007-03-21 | 东北农业大学 | Active bifidobacteria contained milk powder and its preparation method |
| CN101873806A (en) * | 2007-11-26 | 2010-10-27 | 雀巢产品技术援助有限公司 | Age-tailored infant nutrition system |
-
1995
- 1995-07-31 CN CN 95111712 patent/CN1128103A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305383C (en) * | 2003-11-03 | 2007-03-21 | 东北农业大学 | Active bifidobacteria contained milk powder and its preparation method |
| CN101873806A (en) * | 2007-11-26 | 2010-10-27 | 雀巢产品技术援助有限公司 | Age-tailored infant nutrition system |
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