CN112816437A - Food analyzer based on total reflection refraction method - Google Patents
Food analyzer based on total reflection refraction method Download PDFInfo
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- CN112816437A CN112816437A CN202110200984.8A CN202110200984A CN112816437A CN 112816437 A CN112816437 A CN 112816437A CN 202110200984 A CN202110200984 A CN 202110200984A CN 112816437 A CN112816437 A CN 112816437A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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Abstract
一种基于全反射折光法的食品分析仪，其特征在于：它包括单波长LED光源(1)，所述单波长LED光源(1)发出的单色光的光路上装有针孔板(2)，所述单色光在针孔板(2)的小孔处发生衍射光后形成一定角度的发散光进入高折射率棱镜(3)，在高折射率棱镜(3)和被测液体(4)的耦合面处发生全反射作用形成全反光反射回高折射率棱镜(3)，反射光线照射在线阵CCD采集系统(8)敏感面上形成黑白图像；本分析仪可完全实现现有食品分析仪的功能，并且还增加了物质折射率的测量功能，仪器结构简单，可靠性高，成本低廉。
A food analyzer based on total reflection and refraction method is characterized in that: it comprises a single-wavelength LED light source (1), and a pinhole plate (2) is installed on the optical path of the monochromatic light emitted by the single-wavelength LED light source (1). ), the monochromatic light is diffracted at the small holes of the pinhole plate (2) and then forms a certain angle of divergent light into the high-refractive index prism (3), where the high-refractive index prism (3) and the measured liquid ( 4) Total reflection occurs at the coupling surface of the CCD to form a total reflection back to the high-refractive index prism (3), and the reflected light irradiates the sensitive surface of the linear array CCD acquisition system (8) to form a black and white image; this analyzer can completely realize the existing food The function of the analyzer is also increased, and the measurement function of the refractive index of the material is also added. The instrument has a simple structure, high reliability and low cost.
Description
Technical Field
The invention belongs to the technical field of material analysis instruments, and particularly relates to a food analyzer based on a total reflection refraction method.
Background
The refractive index of a liquid is one of the important physical properties of a liquid, and the refractometry is one of the detection methods commonly used in food analysis and food safety detection. The purity of quality, doping condition and the like can be known by the aid of the refractive index. With the development of science and technology, the rapid measurement of the refractive index of liquid is widely used in many fields.
With the development of social economy, the existing handheld visual refractometer in the field of food analysis can not meet the market demand more and more, at present, the handheld visual refractometers existing in a large number in the market are simple pure optical instruments, and the defects of complex operation, low measurement speed, high operation strength, low measurement precision and the like are caused by adopting visual aiming to manually read.
The colorimeter method is a common analysis method, can be used for quantitative or qualitative analysis of substances, and is widely applied to the fields of chemistry, clinical medicine, life science, food, pharmacy, environmental monitoring and the like. Therefore, the photoelectric colorimeter is a basic instrument widely applied to food safety detection and biochemical medicine inspection, and has a wide market. The existing food analyzer is designed based on the principle of colorimetry, but can only analyze components and contents of a sample and cannot measure the refractive index of an object. Therefore, the range of applications of the food analyzer is limited.
Disclosure of Invention
The invention aims to provide a food analyzer based on a total reflection refraction method aiming at the defects of the existing food analyzer, which can completely realize the functions of the existing food analyzer and also adds the function of measuring the refractive index of a substance, and has the advantages of simple instrument structure, high reliability and low cost.
Technical scheme
In order to achieve the technical purpose, the food analyzer based on the total reflection refraction method comprises a single-wavelength LED light source, wherein a pinhole plate is arranged on a light path of monochromatic light emitted by the single-wavelength LED light source, the monochromatic light forms divergent light with a certain angle after being diffracted at small holes of the pinhole plate and enters a high-refractive-index prism, total reflection is carried out at a coupling surface of the high-refractive-index prism and a liquid to be measured to form a total reflection and reflection back to the high-refractive-index prism, and the reflected light irradiates on a sensitive surface of a linear array CCD (charge coupled device) acquisition system to form a black-and-white image;
white light emitted by the LED light source is changed into parallel light through the condensing lens, the parallel light irradiates on a detected liquid on the surface of the high-refractive-index prism 3, a part of transmitted light absorbed by the detected liquid 4 is split through the light splitting element 5 and then enters the imaging lens and is focused on one part of photosensitive surface of the area array CCD acquisition system through the imaging lens, and the other part of transmitted light directly enters the imaging lens and is focused on the other part of photosensitive surface of the area array CCD through the imaging lens.
Further, the single-wavelength LED light source emits single-color light with the wavelength of 589 nm.
Further, the aperture of the pinhole plate is 0.5 micron.
Further, the linear array CCD acquisition system is controlled by an ARM controller.
Advantageous effects
The invention provides a food analyzer based on a total reflection refraction method, which can completely realize the functions of the existing food analyzer and also adds the function of measuring the refractive index of a substance, and has the advantages of simple instrument structure, high reliability and low cost. Can realize the colorimetric measurement of the refractive index and the luminosity of the substance. The device has the advantages that the device can take pictures through videos, and can observe defects such as bubbles or impurities in the measured substance, so that the consistency and the correctness of the measurement result are ensured. The method is widely applied to quality measurement of transparent products, and can be used for qualitative and quantitative analysis of a plurality of substances which can generate color reaction.
Drawings
FIG. 1 is a light path diagram in an embodiment of the present invention.
FIG. 2 is a schematic diagram illustrating the calculation principle of the refractive index of the measured liquid according to the embodiment of the invention.
FIG. 3 is a schematic diagram of different parts of a sensor of an area array CCD acquisition system according to an embodiment of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "inside", "outside", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, which are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and operate, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted" and "connected" are to be interpreted broadly, e.g., as being either fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The present invention will be described in further detail below with reference to specific embodiments and with reference to the attached drawings.
Examples
As shown in fig. 1, a total reflection refractometry-based food analyzer includes a single-wavelength LED light source 1, and in this embodiment, the single-wavelength LED light source 1 emits monochromatic light with a wavelength of 589 nm. The light path of the monochromatic light emitted by the single-wavelength LED light source 1 is provided with a pinhole plate 2, and in the embodiment, the aperture of the pinhole plate 2 is 0.5 micrometer. The monochromatic light is diffracted at the small hole of the pinhole plate 2 to form divergent light with a certain angle, the divergent light enters the high-refractive-index prism 3, the total reflection is performed at the coupling surface of the high-refractive-index prism 3 and the liquid 4 to be detected to form total reflection, the total reflection is reflected back to the high-refractive-index prism 3, and the reflected light irradiates the sensitive surface of the linear array CCD acquisition system 8 to form a black-and-white image; the linear array CCD acquisition system 8 is controlled by an ARM controller.
White light emitted by the LED light source 10 is changed into parallel light through the condenser lens 9, the parallel light irradiates the liquid 4 to be detected on the surface of the high-refractive-index prism 3, a part of transmitted light absorbed by the liquid 4 to be detected is split through the light splitting element 5 and then enters the imaging lens 6 and is focused on one part of the photosensitive surface of the area array CCD acquisition system 7 through the imaging lens 6, and the other part of transmitted light directly enters the imaging lens 6 and is focused on the other part of the photosensitive surface of the area array CCD7 through the imaging lens 6.
In the embodiment, after monochromatic light with the wavelength of 589nm emitted by a single-wavelength LED light source 1 directly irradiates a pinhole plate 2 with the aperture of 0.5 micron, diffraction light is generated at a pinhole, divergent light with a certain angle enters a high-refractive-index prism 3, total reflection is generated at a coupling surface of the high-refractive-index prism 3 and a measured liquid 4, after total reflection is reflected back to the high-refractive-index prism 3, a reflected image is finally irradiated on a sensitive surface of a linear array CCD acquisition system 8, and a black and white image is formed; the ARM controller controls the linear array CCD acquisition system 8 to acquire a total reflection image of the measured liquid 4 for determining a total reflection angle and calculating the refractive index of the measured object corresponding to the wavelength,
as shown in fig. 2, the calculation formula of the refractive index is: the ABCD is a refractive prism with refractive index n1. On the AB surface is the object to be measured with refractive index n2The incident angle of the total reflection critical light on the measurement surface AB is alpha, the incident angle of the light on the BC surface is beta, the refraction angle is i, the upper vertex angle of the refraction prism is phi, which is obtained from the law of refraction:
the angle i can be calculated by an arctangent function obtained by dividing the distance from the boundary to the center of the linear array CCD acquisition system 8 by the distance from the light spot to the surface of the linear array CCD acquisition system 8.
And simultaneously controlling the photoelectric colorimetric measuring part to measure the photometric quantity of the liquid sample.
Photometric measurement its calculation formula: a ═ lg (I/I)0)
A-absorbance of the liquid to be detected; i-the intensity of light transmitted through the liquid to be measured; i is0The intensity of light transmitted through the cuvette without the liquid to be tested.
When the photoelectric colorimetry to transparent liquid is measured, the white light that instrument LED light source 10 sent becomes the parallel light through condensing lens 9, directly shines on the measured liquid 4 on the high refractive index prism 3 surface, through the absorptive transmission light of sample, is divided into two parts: one part of the light is split by a light splitting element 5{ the light splitting can be realized by a triangular prism or a grating }, and then is focused on part of a photosensitive surface of an area array CCD acquisition system 7 by an imaging lens 6, and absorption data of monochromatic light is obtained by a data acquisition system and is used for realizing the measurement of a photoelectric colorimeter; and the other part of light is focused on the other part of photosensitive surface of the area array CCD acquisition system 7 through the imaging lens 6, and a liquid image on the surface of the high-refractive-index prism 3 is obtained through the data acquisition system and is used for detecting bubbles and impurities in the liquid on the surface of the high-refractive-index prism 3 when a sample is loaded.
The ARM controller controls the area array CCD acquisition system 7 to firstly realize image acquisition of a tested sample, processes and analyzes the image to obtain defect state evaluation of the tested sample, prompts a user to eliminate interference factors such as bubbles and impurities, continues calculation and measurement of the refractive index and the photometric value of an object and the content of the sample, and finally controls the touch liquid crystal color screen to realize the refractive index and the photometric value of the tested sample and the content of the component of the tested object.
The ARM controller controls the area array CCD collection system to firstly realize image collection of a tested sample, processes and analyzes the image to obtain defect state evaluation of the tested sample, prompts a user to eliminate interference factors such as bubbles and impurities, continues calculation and measurement of the refractive index and the photometric value of an object and the content of the sample, and finally controls the touch liquid crystal color screen to realize the refractive index and the photometric value of the tested sample and the content of the component of the tested object. The different parts of the area array CCD sensor are shown in figure 3. The light sensing part a of the area array CCD is used for receiving the monochromatic light after light splitting, namely the light of red, orange, yellow, green, blue and purple, and is used for colorimeter measurement of required wavelength; the rest photosensitive part b of the area array CCD is used for imaging the object to be detected and detecting the defect of the object.
Claims (4)
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| CN202110200984.8A CN112816437A (en) | 2021-02-23 | 2021-02-23 | Food analyzer based on total reflection refraction method |
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2021
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| JPH0658874A (en) * | 1992-08-11 | 1994-03-04 | Nippondenso Co Ltd | Liquid property detector |
| US5477318A (en) * | 1992-09-16 | 1995-12-19 | Nippondenso Co., Ltd. | Apparatus for detecting a property of a liquid |
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