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CN112400903A - Beauveria bassiana and cyenopyrafen compound insecticidal composition - Google Patents

Beauveria bassiana and cyenopyrafen compound insecticidal composition Download PDF

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Publication number
CN112400903A
CN112400903A CN202011385067.3A CN202011385067A CN112400903A CN 112400903 A CN112400903 A CN 112400903A CN 202011385067 A CN202011385067 A CN 202011385067A CN 112400903 A CN112400903 A CN 112400903A
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cyenopyrafen
beauveria bassiana
suspending agent
mites
zju435
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夏玉先
隆静
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Chongqing Gubaiao Biological Research Institute Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles

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  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
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Abstract

The invention belongs to the technical field of biocides for fungus microorganisms, and particularly relates to a compound composition for killing tetranychus urticae. The tetranychicide composition comprises beauveria bassiana and cyenopyrafen. The pesticide has good effect of preventing and treating spider mites.

Description

Beauveria bassiana and cyenopyrafen compound insecticidal composition
Technical Field
The invention belongs to the technical field of biocides containing fungus microorganisms, and particularly relates to a Beauveria bassiana and cyenopyrafen compound insecticidal composition.
Background
Mites belong to a class of small animals of the class of the subclass Guangdong-web of the phylum Arthropoda, Arachnida. The mites are generally oval or round cysts with a combination of head, chest and abdomen, generally flat back and abdomen, generally about 0.1-0.4mm in body size, and most species are less than 2mm in diameter. The body of the worm is composed of an oval body and a jaw body in front of the oval body, a lower jaw plate is arranged below the center of the jaw body, and a pair of chela is arranged on the back of the jaw body and is used as a feeding organ. About 30000 species of mites have been found in the world, second only to insects. Mites are widely distributed, rapidly propagated, parthenogenesis is possible, life styles are diverse, adaptability to the environment is strong, and the mites can live in various environments ("recent progress of mite research in our country, wu ma · arbuli, etc., biological report, vol 44, No. 4, page 12, left column, lines 1-8, left column, lines 1-6, publication day 2009, month 12, 31").
According to the nature of food, the mites can be divided into phytophagous mites and carnivorous mites, and the phytophagous mites mainly comprise tetranychus, gall mites, pink mites, tarsal mites, pyemotes, dwarf pyemotes, tetranychus, Pukotes, root mites, and onychomycosis, and are harmful to piercing and sucking or chewing, and most of the mites are destructive to human production. Tetranychus is one of five worldwide pests (fruit fly, peach aphid, chilo suppressalis, scabies and tetranychus). The spider mites suck the chlorophyll of the plants to cause green spots to fade, and the leaves are yellowed and shed. In recent years, single chemical agents are adopted for human beings to control pest control measures once, so that spider mites are promoted from secondary pests to main pests. Spider mites have become a prominent problem in agriculture and forestry production (the recent progress in mite research in our country, wu ma er arbulimu et al, biological bulletin, vol 44, No. 4, page 13, left column, No. 3, No. 1 to No. 4, No. 10, published 2009, No. 12 and 31).
There are also mite-controlling methods using microorganisms, such as Shiweifen, which discloses a Beauveria bassiana Bb2860 capable of infecting spider mite eggs (research on microbial control of mite-killing plants by biocontrol fungi, Shiweifen, doctor's academic thesis of Zhejiang university, 2007, paragraph 2, lines 1-5 of Abstract, published 2007, 10-15.10.2007). However, compared with chemical pesticides, microorganisms have unsatisfactory mite control effects, poor quick-acting properties and unstable pesticide effects.
Disclosure of Invention
In view of the above, the invention aims to provide a tetranychus-killing compound composition.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the compound composition for killing tetranychus urticae comprises beauveria bassiana and cyenopyrafen.
The tetranychicide refers to the ability of a substance to increase the mortality rate or inhibit the growth rate of an animal of the family spider-class, eurycotina, order acarina, or spider mite.
In the research process, the inventor unexpectedly finds that the beauveria bassiana and the cyenopyrafen have a remarkable synergistic effect on the tetranychus urticae.
Further, the beauveria bassiana is beauveria bassiana ZJU 435.
Further, the proportion of beauveria bassiana and cyenopyrafen is 1 x 400 hundred million spores to 10 x 400 hundred million spores: 0.3-3g cyenopyrafen.
Further, the proportion of beauveria bassiana and cyenopyrafen is 7 x 400 hundred million spores: 1.2g cyenopyrafen.
The invention also aims at protecting the pesticide combination for killing the tetranychus, and the active ingredients of the pesticide combination comprise beauveria bassiana and cyenopyrafen.
Further, the beauveria bassiana is beauveria bassiana ZJU 435.
Further, the proportion of beauveria bassiana and cyenopyrafen is 1 x 400 hundred million spores to 10 x 400 hundred million spores: 0.3-3g cyenopyrafen.
Further, the proportion of beauveria bassiana and cyenopyrafen is 7 x 400 hundred million spores: 1.2g cyenopyrafen.
The invention has the beneficial effects that:
the pesticide has good effect of preventing and treating spider mites.
The beauveria bassiana and the cyenopyrafen have a synergistic effect on spider mites.
The medicament has stable drug effect.
The medicament of the invention has good quick-acting property.
The drug pest of the invention is not easy to generate drug resistance.
Detailed Description
The examples are provided for better illustration of the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art should make insubstantial modifications and adaptations to the embodiments of the present invention in light of the above teachings and remain within the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
The following 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent is produced by Chongqing Juxin bioengineering company Limited (the spore rate is 97.6%);
the following 30% cyenopyrafen suspension was produced by Nippon chemical Co.
Indoor live test and compound combined toxicity test
Detecting the toxicity of the beauveria bassiana ZJU435 oil suspending agent, the pyrauxifen suspending agent and the compound composition (see table 1) thereof on tetranychus cinnabarinus, and meanwhile, calculating the cotoxicity coefficient, wherein the test result is shown in table 1;
a test insect source: tetranychus cinnabarinus, the seedling of kidney bean in plant base of Chongqing university, the temperature is 27 +/-1 ℃, the relative humidity is 60-80%, and the photoperiod is 16:8h (L: D).
Reagent to be tested: 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent is diluted by 500 times, and the concentration is prepared to be 2 multiplied by 107Spores/ml; diluting 30% cyenopyrafen suspending agent by 2000 times, and mixing the beauveria bassiana oil suspending agent diluent and the 30% cyenopyrafen suspending agent diluent according to the volume ratio shown in the table 1;
the test method comprises the following steps:
adhering a 2cm multiplied by 2cm double-sided tape to one end of a glass slide by adopting a slide dipping method recommended by FAO (1980), slightly picking up the mites with a small brush pen to adhere the backs of the mites to the tape (the mites cannot adhere feet, tentacles and mouthparts), adhering 4 rows of the mites, and adhering 10 heads of each row;
putting the glass slide with 40 heads of red spiders into a clean and nontoxic large culture dish, putting a moisture-preserving cotton ball in the culture dish for moisture preservation, covering the culture dish, placing the culture dish at room temperature for 2 hours, then checking the adhesion condition of the red spiders under a binocular dissecting mirror, removing dead and inactive individuals, and counting the number of live spiders as the test base number;
respectively immersing the counted number of slides into liquid medicines with different concentrations, slightly shaking for 5s, taking out, absorbing redundant liquid medicines by using absorbent paper, putting the liquid medicines into a culture dish, putting a moisturizing cotton ball into the culture dish, covering the cotton ball, putting the culture dish into an incubator with the temperature of 27 +/-1 ℃, the relative humidity of 75% and the illumination L: D of 16:8, checking the death condition under a binocular dissecting mirror after 48h, slightly touching the mite body by using a writing brush, considering the immobile person with the chela as dead, and selecting the effective concentration according to the investigation result;
and setting clear water treatment as a blank control; each treatment was set to 3 replicates.
Data processing:
calculating a virulence regression equation by using POLO Plus software, wherein the calculation parameters comprise: slope b value and standard error, chi-squared, degree of freedom, LC50Values and 95% confidence limits.
The combined virulence evaluation method comprises the following steps:
adopting a Sun Yunpei co-toxicity coefficient method:
virulence index (TI) ═ LC of standard agents50LC of test agent50)×100
Actual Toxicity Index (ATI) of mixed preparation, LC for single use of A-agent50LC mixed with medicament A and B50)×100
Mixture theoretical virulence index (TTI) ═ ti (a) × a + ti (b) × b
Wherein a represents the percentage of the medicament A in the mixture, and B represents the percentage of the medicament B in the mixture.
The cotoxicity coefficient CTC is (mixed agent actual virulence index ATI/mixed agent theoretical virulence index TTI) x 100;
according to the regulation of agricultural chemical examination institute of Ministry of agriculture, the judgment standard of the synergy of the mixture formula is as follows: the co-toxicity coefficient is more than 120, so that the synergistic effect is achieved; the co-toxicity coefficient is 80-120, which is additive effect; the co-toxicity coefficient is less than 80, and the antagonism is achieved;
except different medicaments, the treatment modes of the groups are the same; the results are shown in Table 1.
TABLE 1 Combined virulence test results
Figure BDA0002809416520000031
Figure BDA0002809416520000041
As can be seen from the table 1, in the range of the volume ratio of 500 times of the beauveria bassiana ZJU435 oil suspending agent to 2000 times of the cyenopyrafen 2000 diluent, the two are compounded, the co-toxicity coefficient is more than 120, and the synergistic effect is shown; when the volume ratio of the two is 7:4, the synergistic effect is most remarkable, and the co-toxicity coefficient can reach 458.8. Therefore, the beauveria bassiana ZJU435 and the cyenopyrafen have a synergistic effect on tetranychus cinnabarinus.
Example 1
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 10:1, mixing for standby.
Example 2
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 9: 2, mixing for standby.
Example 3
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 8: 3, mixing for later use.
Example 4
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 7:4, mixing for standby.
Example 5
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 6: 5, mixing for standby.
Example 6
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 5: 6, mixing for standby.
Example 7
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 4: 7, mixing for standby.
Example 8
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 3: 8, mixing for later use.
Example 9
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 2: 9, mixing for standby.
Example 10
Respectively diluting 500 times of 100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent, diluting 2000 times of solution of 30% cyenopyrafen suspending agent, and mixing the beauveria bassiana ZJU435 oil suspending agent diluent and cyenopyrafen suspending agent diluent according to the volume ratio of 1:10 mixing for standby.
Example 11
100 hundred million spores/ml beauveria bassiana ZJU435 oil suspending agent is diluted by 500 times, and the concentration is prepared to be 2 multiplied by 107Spores/ml for use.
Example 12
And diluting 2000 times of solution by using 30% cyenopyrafen suspending agent for later use.
Indoor compatibility test
The wettable powder of examples 1-11 was incubated in an incubator at 37 ℃ and then the spore germination rates were examined on day 3 and 14, respectively, to verify whether cyenopyrafen had an effect on the germination of beauveria bassiana spores;
the spore germination rate detection method comprises the following steps: each preparation sample was diluted with sterile water to a spore content of 1X 107Taking 50uL spore suspension, uniformly coating the suspension on an 1/4SDAY plate, and detecting the germination rate after culturing for 14h at 28 ℃;
the above groups were administered in the same manner except for the different agents.
TABLE 2 germination Rate test results
Figure BDA0002809416520000051
Figure BDA0002809416520000061
Remarking: lower case differences indicate significant differences at the 0.05 level and upper case differences indicate significant differences at the 0.01 level.
As can be seen from Table 2, the germination rates of examples 1-10 were not significantly different from that of example 11. Therefore, the compatibility of cyenopyrafen and beauveria bassiana ZJU435 is good.
And (3) field application test: panonychus citri field test
The panonychus citri field control test was performed with the combinations of examples 1-12 while setting a blank control (i.e. no application of any agent);
the method specifically comprises the following steps: the test is carried out in a Pedun district of Chongqing city in a Padente agricultural science and technology garden in the citrus field, the variety is elegant No. 1, and the field planting is carried out for 3 years;
the drug is applied for 1 time in the initial stage of the panonychus citri, and the number of mites is investigated before, 3 days, 7 days and 21 days after the drug application;
respectively selecting 1 branch tip in the east, west, south and north directions of the tree in each cell, marking 5 leaves on each tip, and surveying and recording the number of the active mites on 20 leaves on 4 tips;
and (3) calculating the control effect according to the following formula:
Figure BDA0002809416520000062
Figure BDA0002809416520000063
wherein PT0 represents before administration, PT1 represents after administration, PT represents the drug-treated area, and CK represents the blank control group;
except different medicaments, the treatment modes of the groups are the same; the results are shown in Table 3.
TABLE 3 field test results for Panonychus citri
Figure BDA0002809416520000064
Figure BDA0002809416520000071
Remarking: capital letters differ significantly at the 0.05 level, lowercase letters differ significantly at the 0.01 level; the application amount of each treatment is converted into the cost of the original drug and is consistent, and the application amount is set to be 1 yuan/15L of water.
As can be seen from table 3, the control effect of the combination of examples 1 to 10 on panonychus citri is significantly improved at the same cost compared with those of example 11 (single dose) and example 12 (single dose). Therefore, the compound medicament disclosed by the invention has an excellent control effect on panonychus citri, and is good in quick-acting property and long in lasting period.
And (3) field application test: eggplant tetranychus urticae field test
A field control test of tetranychus urticae was carried out using the combinations of examples 1-12, while a blank control group (i.e. no agent applied) was set;
the method specifically comprises the following steps: the test is carried out in an eggplant field of a Yuxi vegetable research and development center base in Chongqing;
the application is carried out 1 time in the initial stage of the two-spotted spider mite of eggplant, and the number of the mites is investigated before the application, 3 days, 7 days and 21 days after the application;
randomly marking 10 eggplants in each cell, and marking 5 leaves in each eggplant;
the number of active mites on 50 leaves was recorded in a total survey.
And (3) calculating the control effect according to the following formula:
Figure BDA0002809416520000072
Figure BDA0002809416520000073
wherein PT0 represents before administration, PT1 represents after administration, PT represents the drug-treated area, and CK represents the blank control group;
except different medicaments, the treatment modes of the groups are the same; the results are shown in Table 4.
TABLE 4 field test results for two-spotted spider mite eggplant
A：B Test drugs The dosage of the water is ml/15L 3d control effect% 7d control effect% Control effect for 21 days%
10:1 Example 1 24.69 76.11bB 90.02abA 81.11abA
9:2 Example 2 17.78 82.16abAB 91.82abA 80.80abA
8:3 Example 3 13.89 84.27abA 90.99abA 83.12abA
7:4 Example 4 11.40 86.17aA 94.01aA 84.00aA
6:5 Example 5 9.66 80.33abAB 92.22abA 83.11abA
5:6 Example 6 8.39 81.27abAB 90.17abA 79.92abA
4:7 Example 7 7.41 83.27abA 91.77abA 80.05abA
3:8 Example 8 6.63 81.06abA 91.06abA 76.19bAB
2:9 Example 9 6.01 82.71abA 90.03abA 80.98abA
1:10 Example 10 5.49 80.21abAB 91.04abA 82.17abA
1:0 Example 11 55.56 62.81cC 73.09cB 68.99cC
0:1 Example 12 5.56 85.11aA 88.35bA 70.08cB
Remarking: capital letters differ significantly at the 0.05 level, lowercase letters differ significantly at the 0.01 level; the application amount of each treatment is converted into the cost of the original drug and is consistent, and the application amount is set to be 1 yuan/15L of water.
As can be seen from Table 4, compared with the single dose of the compound of the example 11 and the single dose of the example 12, the compound combination of the examples 1 to 10 has obviously improved control effect on the tetranychus solani at the same cost. Therefore, the compound combination disclosed by the invention has an excellent control effect on the two-spotted spider mites of eggplants, and is good in quick-acting property and long in lasting period.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (7)

1. A tetranychicide composition, characterized in that the active ingredients comprise beauveria bassiana and cyenopyrafen.
2. The composition of claim 1, wherein the beauveria bassiana is beauveria bassiana ZJU 435.
3. The composition according to claim 1 or 2, wherein the ratio of beauveria bassiana to cyenopyrafen is 1 x 400 million spores to 10 x 400 million spores: 0.3-3g cyenopyrafen.
4. The composition of claim 3, wherein the ratio of beauveria bassiana to cyenopyrafen is 7 x 400 hundred million spores: 1.2g cyenopyrafen.
5. The tetranychicide is characterized in that active ingredients comprise beauveria bassiana and cyenopyrafen.
6. The medicament of claim 5, wherein the ratio of beauveria bassiana to cyenopyrafen is 1 x 400 million spores to 10 x 400 million spores: 0.3-3g cyenopyrafen.
7. The medicament of claim 6, wherein the ratio of beauveria bassiana to cyenopyrafen is 7 x 400 hundred million spores: 1.2g cyenopyrafen.
CN202011385067.3A 2020-12-01 2020-12-01 Beauveria bassiana and cyenopyrafen compound insecticidal composition Pending CN112400903A (en)

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JIN‑CUI CHEN等: "Field evolved resistance and cross resistance of the two spotted spider mite, Tetranychus urticae, to bifenazate, cyenopyrafen and SYP 9625", 《EXPERIMENTAL AND APPLIED ACAROLOGY》 *
佚名: "真菌农药再添猛将，球孢白僵菌ZJU435强势登场", 《HTTPS://WWW.SOHU.COM/A/401348961_100204915》 *
彭国雄等: "杀虫真菌对草地贪夜蛾不同虫态的室内活性", 《中国生物防治学报》 *
程岩等: "腈吡螨酯（cyenopyrafen）的合成与杀螨活性", 《现代农药》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113925056A (en) * 2021-08-30 2022-01-14 广东中迅农科股份有限公司 Acaricidal composition
CN117178791A (en) * 2023-09-25 2023-12-08 南京金色庄园农产品有限公司 A biological control method for fruit and vegetable pest mites

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