[go: up one dir, main page]

CN111918965A - 一种胎儿游离核酸的富集方法及其应用 - Google Patents

一种胎儿游离核酸的富集方法及其应用 Download PDF

Info

Publication number
CN111918965A
CN111918965A CN201880091896.5A CN201880091896A CN111918965A CN 111918965 A CN111918965 A CN 111918965A CN 201880091896 A CN201880091896 A CN 201880091896A CN 111918965 A CN111918965 A CN 111918965A
Authority
CN
China
Prior art keywords
nucleic acid
fetal
detection
free nucleic
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880091896.5A
Other languages
English (en)
Inventor
杨林
王逸丛
高雅
陈芳
张翔涵
于靖
张海萍
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BGI Shenzhen Co Ltd
Original Assignee
BGI Shenzhen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BGI Shenzhen Co Ltd filed Critical BGI Shenzhen Co Ltd
Publication of CN111918965A publication Critical patent/CN111918965A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

一种胎儿游离核酸富集方法，包括对含胎儿游离核酸的样品进行PCR扩增，获得胎儿游离核酸富集产物；PCR扩增包括先采用引物对的正向或反向引物对样品进行第一次线性扩增，然后再向第一次扩增产物中加入另一引物进行第二次指数扩增，即获得胎儿游离核酸富集产物。

Description

PCT国内申请，说明书已公开。

Claims (19)

  1. PCT国内申请，权利要求书已公开。
CN201880091896.5A 2018-04-28 2018-04-28 一种胎儿游离核酸的富集方法及其应用 Pending CN111918965A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2018/085080 WO2019205132A1 (zh) 2018-04-28 2018-04-28 一种胎儿游离核酸的富集方法及其应用

Publications (1)

Publication Number Publication Date
CN111918965A true CN111918965A (zh) 2020-11-10

Family

ID=68294760

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880091896.5A Pending CN111918965A (zh) 2018-04-28 2018-04-28 一种胎儿游离核酸的富集方法及其应用

Country Status (2)

Country Link
CN (1) CN111918965A (zh)
WO (1) WO2019205132A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110846382B (zh) * 2019-11-29 2023-02-28 北京科迅生物技术有限公司 胎儿游离dna的富集方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103403183B (zh) * 2011-06-29 2014-10-15 深圳华大基因医学有限公司 胎儿遗传异常的无创性检测
WO2016095736A1 (zh) * 2014-12-18 2016-06-23 深圳华大基因研究院 一种基于多重pcr的目标区域富集方法和试剂
CN107083440A (zh) * 2017-06-13 2017-08-22 深圳华大智造科技有限公司 一种检测染色体非整倍性的试剂盒及其制备方法和应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2467460A1 (en) * 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
GB201410646D0 (en) * 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103403183B (zh) * 2011-06-29 2014-10-15 深圳华大基因医学有限公司 胎儿遗传异常的无创性检测
WO2016095736A1 (zh) * 2014-12-18 2016-06-23 深圳华大基因研究院 一种基于多重pcr的目标区域富集方法和试剂
CN107083440A (zh) * 2017-06-13 2017-08-22 深圳华大智造科技有限公司 一种检测染色体非整倍性的试剂盒及其制备方法和应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
XIAOLI LOU ET AL.: "A novel Alu-based real-time PCR method for the quantitative detection of plasma circulating cell-free DNA: Sensitivity and specificity for the diagnosis of myocardial infarction" *
孙艺祯 等: "肾综合征出血热患者血浆cf-DNA 特征及其深度测序分析" *

Also Published As

Publication number Publication date
WO2019205132A1 (zh) 2019-10-31

Similar Documents

Publication Publication Date Title
US9890375B2 (en) Isolated oligonucleotide and use thereof in nucleic acid sequencing
JP6739339B2 (ja) カバーされた配列変換ｄｎａおよび検出方法
JP6321757B2 (ja) Ｎｇｓライブラリ作製用プライマーセットおよびこれを用いたｎｇｓライブラリ作製法およびキット
JP2022036975A (ja) ナノポア技術を用いた短いｄｎａ断片の迅速な配列決定
WO2018112806A1 (zh) 将线性测序文库转换为环状测序文库的方法
EP3607065B1 (en) Method and kit for constructing nucleic acid library
RU2013103795A (ru) Новый способ пцр-секвенирования и его применение в генотипировании hla
WO2012159564A1 (zh) 甲基化高通量检测方法
JP7539770B2 (ja) ゲノム再編成検出のための配列決定方法
WO2016181128A1 (en) Methods, compositions, and kits for preparing sequencing library
JP2015516814A (ja) 標的化されたｄｎａの濃縮および配列決定
CN103571822B (zh) 一种用于新一代测序分析的多重目的dna片段富集方法
CN107709557A (zh) 基于lna的突变型富集下一代测序测定
CN108251503A (zh) 一种快速构建链特异性rna高通量测序文库的方法
CN106754903A (zh) 一种用于人类线粒体的全基因组扩增的引物对及方法
CN108707648A (zh) Pkd1基因和pkd2基因突变的检测方法和检测试剂盒
US20210102246A1 (en) Genetic test for detecting congenital adrenal hyperplasia
CN106929504A (zh) 检测急性早幼粒细胞白血病相关融合基因的试剂盒
TW201321520A (zh) 用於病毒檢測的方法和系統
WO2017113148A1 (zh) 检测急性早幼粒细胞白血病相关融合基因的试剂盒
CN111918965A (zh) 一种胎儿游离核酸的富集方法及其应用
WO2012083845A1 (zh) 用于除去测序文库中载体片段的方法及其用途
US10590451B2 (en) Methods of constructing a circular template and detecting DNA molecules
US20180291369A1 (en) Error-proof nucleic acid library construction method and kit
CN106967833B (zh) 用于二倍体a基因组棉种和/或四倍体棉种鉴定的引物及其pcr鉴定方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20201110

RJ01 Rejection of invention patent application after publication