CN111804427A - Collection structure for magnetic particles - Google Patents

Abstract

The invention discloses a collecting structure for magnetic particles, which is characterized by comprising a pipe body, a first magnetic block body and a second magnetic block body, wherein the second magnetic block body and the first magnetic block body are adjacently arranged, the right end face of the first magnetic block body and the right end face of the second magnetic block body are respectively contacted with the pipe body or the distance between the right end face of the first magnetic block body and the pipe body is less than 2mm, the polarization direction of the first magnetic block body and the polarization direction of the second magnetic block body are opposite, the polarization direction of the first magnetic block body and the polarization direction of the second magnetic block body are both vertical to the axial direction of the pipe body, and a gap between the first magnetic block body and the second magnetic block body is parallel to the axial direction of the pipe body. According to the invention, a stronger magnetic field is generated inside the pipe body, so that magnetic particles can be collected more effectively and more stably.

Description

Collection structure for magnetic particles

technical field

The invention belongs to the technical field of magnetic particles, and in particular relates to a collection structure for magnetic particles.

Background technique

At present, many technologies have been developed, such as using micromagnetic particles as carriers to carry out various reactions in solution, and separating the reaction products from the solution by separating magnetic microspheres, including immunoassays, methods for extracting and analyzing nucleic acids, and analyzing Methods for proteins or ligands, combinatorial chemistry, etc.

For example, in various immunoassay methods widely used to detect various early diseases or to detect trace substances, magnetic particle technology with antigens or antibodies is used, which provides high sensitivity and allows simple B /F Detach operation. B/F separation refers to the step of separating the unreacted material and the antigen-antibody reaction product by removing the unreacted material from the reaction mixture in the reaction vessel, and repeating the washing operation including supplying and removing the washing solution . When using magnetic particles with antigens or antibodies, the magnetic particles and the sample are mixed together for an antigen-antibody reaction, and then magnetic particles containing the generated immune complexes can be quickly collected, and unreacted antigens or unreacted antigens can be separated and washed. Antibody.

For these methods, improving the recovery rate of magnetic particles is an important issue. Although the micromagnetic particles can perform various reactions with high efficiency, the micromagnetic particles tend to be suspended in the solution, resulting in lower recovery rates, and the magnetic particles may be lost during washing, separation, etc., resulting in lower reliability of the measurement results. Especially in the case of immunoassays, where multiple B/F separations are performed, the loss of magnetic particles can greatly affect the measured value.

An existing magnetic particle collection device is shown in Figure 1 of the description, which includes a plurality of magnets with alternately arranged magnetic poles and a suction head with a liquid flow direction parallel to the arrangement direction of the magnets. The alternately arranged magnets are generated inside the suction head. A magnetic field that alternates along the liquid flow direction, the reaction solution in the suction head flows back and forth along the liquid flow direction in the suction head, and the magnetic particles in the reaction solution are adsorbed on the wall of the suction head under the action of the magnetic field, so as to achieve the purpose of collecting magnetic particles . However, although the magnetic field generated by this method in the direction parallel to the surface of the magnet inside the suction head has a strong peak intensity, which can reach 180mT (as shown in Figure 2 of the description), the magnetic field is in the direction of the liquid flow (that is, the axial direction of the suction head). It is strong and weak alternately, the overall magnetic field is still weak, the average magnetic field is only about 90mT, the magnetic force on the magnetic particles is still insufficient, the speed of the liquid flow needs to be reduced to increase the action time of the liquid flow in the magnetic field range, in order to achieve a better performance Magnetic particle collection effect.

Therefore, the existing technology needs to be improved.

SUMMARY OF THE INVENTION

The main purpose of the present invention is to propose a collection structure for magnetic particles, aiming to solve the technical problems mentioned in the background art. A strong magnetic field will be generated inside the tube along the direction parallel to the end face of the magnetic block, and the peak value The intensity can reach 160mT, and the magnetic field intensity changes very little along the axis of the tube body, and the average magnetic field is about 150mT.

A collection structure for magnetic particles of the present invention includes a tube body, a first magnetic block and a second magnetic block, the second magnetic block and the first magnetic block are arranged adjacent to each other, and the right end of the first magnetic block is The surface and the right end surface of the second magnetic block are respectively in contact with the tube body or the distance between the tube body and the tube body is less than 2mm, and the polarization direction of the first magnetic block is opposite to that of the second magnetic block. The polarization direction of the first magnetic block and the polarization direction of the second magnetic block are both perpendicular to the axis direction of the pipe body, and the gap between the first magnetic block body and the second magnetic block body is parallel to the axis direction of the pipe body.

Preferably, the thickness of the slit is 0-5mm.

Preferably, the first magnetic block body is provided with a first space-saving structure on the side close to the tube body, and the second magnetic block body is provided with a second space-avoidance structure at the side close to the pipe body.

Preferably, the first hollow structure is a first chamfered structure, a first rounded structure or a first concave structure.

Preferably, the second hollow structure is a second chamfered structure, a second rounded structure or a second concave structure.

It also includes a clamp structure and a moving mechanism that drives the clamp structure to move. The clamp structure includes a magnet mounting portion, and the moving mechanism includes a drive mechanism, a rack provided with guide rails, and a slider that can slide on the guide rail. The slider is connected to the drive mechanism through a screw rod. The slider is connected with the magnet mounting part, and the magnet mounting part is provided with a first positioning cavity for placing the first magnetic block and a second positioning cavity for placing the second magnetic block.

The collection structure for magnetic particles of the present invention is based on the fact that the gap between the first magnetic block and the second magnetic block is parallel to the axial direction of the tube and both are in contact with the tube or the distance from the tube is less than 2 mm , the first magnetic block and the second magnetic block will generate a strong magnetic field in the pipe body along the direction parallel to the right end face of the magnetic block body, the peak intensity can reach 160mT, and the magnetic field varies greatly along the liquid flow direction of the pipe body Small, the average magnetic field is about 150mT, so that the magnetic particles in the liquid flow can be subjected to a strong magnetic force directed to the right end face of the magnetic block for a long time when flowing along the pipe body, and are quickly adsorbed to the adjacent magnetic block. The inner wall of the tube can achieve the effect of collecting magnetic particles.

Description of drawings

Fig. 1 is the prior art magnetic particle collection device mentioned in the background;

FIG. 2 is a distribution diagram of the magnetic field intensity achieved by the prior art magnetic particle collecting device mentioned in the background;

3 is a three-dimensional schematic diagram of the collection structure for magnetic particles of the present invention;

4 is a first top view of the collection structure for magnetic particles of the present invention;

5 is a second top view of the collection structure for magnetic particles of the present invention;

FIG. 6 is a schematic structural diagram of a first embodiment of a first hollow structure and a second hollow structure in the collection structure for magnetic particles of the present invention;

FIG. 7 is a schematic structural diagram of a second embodiment of a first void-escape structure and a second void-avoidance structure in the collection structure for magnetic particles of the present invention;

FIG. 8 is a schematic structural diagram of a third embodiment of a first air-removing structure and a second air-removing structure in the collection structure for magnetic particles of the present invention;

Fig. 9 is the magnetic field intensity distribution diagram of the collecting structure for magnetic particles of the present invention;

10 is a first three-dimensional schematic diagram of a clamp structure;

FIG. 11 is a second three-dimensional schematic view of the clamp structure.

The realization, functional characteristics and advantages of the present invention will be further described with reference to the accompanying drawings in conjunction with the embodiments.

Detailed ways

It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention. It should be noted that related terms such as "first", "second", etc. may be used to describe various components, but these terms do not limit the component. These terms are only used to distinguish one component from another. For example, a first component could be termed a second component, and a second component could similarly be termed a first component, without departing from the scope of the present invention. The term "and/or" refers to a combination of any one or more of the associated and described items.

As shown in Fig. 3, Fig. 4, Fig. 5, Fig. 3 is a three-dimensional schematic diagram of the collection structure for magnetic particles of the present invention; Fig. 4 is the first top view of the collection structure for magnetic particles of the present invention; Fig. 5 is The second top view of the collection structure for magnetic particles of the present invention; a collection structure for magnetic particles of the present invention includes a tube body 10, a first magnetic block 21 and a second magnetic block 22, and the second magnetic The block and the first magnetic block are arranged adjacently, and the right end face of the first magnetic block and the right end face of the second magnetic block are respectively in contact with the pipe body or the distance between them and the pipe body is less than 2mm, and the polarization of the first magnetic block The direction is opposite to the polarization direction of the second magnetic block. The polarization direction of the first magnetic block and the polarization direction of the second magnetic block are both perpendicular to the axis direction of the tube body. The gap 40 between the blocks is parallel to the axial direction of the pipe body 10 . Wherein, as shown in FIG. 4 , the gap 40 represents the interval between the first magnetic block and the second magnetic block, which can be understood as a plane; the collection structure for magnetic particles of the present invention is based on the first magnetic block The gap with the second magnetic block is parallel to the axis direction of the tube body and the distance between the two and the tube body is less than 2mm, only the first magnetic block and the second magnetic block are used inside the tube body along parallel to the magnetic block. The direction of the right end face will generate a strong magnetic field, the peak strength can reach 160mT, and the magnetic field changes little along the liquid flow direction of the pipe body, the average magnetic field is about 150mT (as shown in Figure 9), and the magnetic field strength is perpendicular to the magnetic field. The direction of the right end face of the block gradually decreases outward. The direction of the magnetic force received by the magnetic particles in the magnetic field is perpendicular to the direction of the magnetic field, and points from the weak magnetic field to the strong magnetic field, so that the magnetic particles in the liquid flow can grow longer when flowing along the pipe body. The time is subjected to a strong magnetic force directed towards the right end face of the magnetic block, and is quickly adsorbed to the inner wall of the tube adjacent to the magnetic block to achieve the effect of collecting magnetic particles.

The polarization direction of the first magnetic block refers to the direction in which the N pole points to the S pole; the polarization direction of the second magnetic block refers to the direction in which the N pole points to the S pole.

Wherein, the axial direction of the pipe body represents the vertical upward direction X or the vertical downward direction Y.

Wherein, the first magnetic block represents a block structure made of magnetic material; the second magnetic block represents a block structure made of magnetic material; the first magnetic block 21 is provided with a first The void structure 51 means that the end face of the first magnetic block 21 close to the tube body 10 is provided with a first void structure 51 , and the first void structure can be a first chamfered structure, a first rounded corner structure or a first inner concave structure. Similarly, a second hollow structure 52 is formed on the side of the second magnetic block close to the tube body 10 , and the second hollow structure can be a second chamfered structure, a second rounded corner structure or a second concave structure.

As shown in FIG. 6 , when the first hollow structure is the first chamfered structure, and the second hollow structure is the second chamfered structure; as shown in FIG. 7 , when the first hollow structure is the first rounded structure, The second hollow structure is a second rounded structure; as shown in FIG. 8 , when the first hollow structure is a first concave structure, the second hollow structure is a second concave structure.

As shown in Fig. 4, preferably, the thickness of the slit is 0-5mm. ; For example, the magnetic field intensity generated when the gap thickness is 2mm is greater than the magnetic field intensity generated when the gap thickness is 0mm. Specifically, as shown in FIG. 10 and FIG. 11 , it also includes a clamp structure and a moving mechanism that drives the clamp structure to move. The clamp structure includes a magnet mounting portion 501 , and the moving mechanism includes a drive mechanism 500 , a frame 502 provided with guide rails 505 and a movable mechanism. The slider 503 slides on the guide rail 505. The slider 503 is connected to the driving mechanism 500 through the screw rod 504. The slider 503 is connected to the magnet mounting part 501. The magnet mounting part 501 is provided with a first place for placing the first magnetic block 21 A positioning cavity, a second positioning cavity for placing the second magnetic block 22 . The magnet mounting part 501 is set based on two positioning cavities, so that the gap between the first magnetic block and the second magnetic block can reach 0-5 mm; in order to achieve stable and efficient collection of micro-magnetic particles. The moving mechanism is arranged in the instrument to smoothly drive the clamp structure to move, so as to realize the function that the right end face of the first magnetic block and the right end face of the second magnetic block are in contact with the tube body or the distance between them is less than 2mm.

The above are only preferred embodiments of the present invention, and are not intended to limit the scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the description and drawings of the present invention, or directly or indirectly applied in other related technical fields , are similarly included in the scope of patent protection of the present invention.

Claims (6)

1. A collection structure for magnetic particles, characterized in that it comprises a pipe body, a first magnetic block and a second magnetic block, the second magnetic block and the first magnetic block are arranged adjacently, and the first magnetic block is arranged adjacent to the first magnetic block. The right end face of the block and the right end face of the second magnetic block are respectively in contact with the tube body or the distance between them is less than 2mm, and the polarization direction of the first magnetic block is opposite to that of the second magnetic block. The polarization direction of the magnetic block body and the polarization direction of the second magnetic block body are both perpendicular to the axis direction of the pipe body, and the gap between the first magnetic block body and the second magnetic block body is parallel to the axis direction of the pipe body. 2 . The collecting structure for magnetic particles according to claim 1 , wherein the thickness of the slit is 0-5 mm. 3 . 3. The collecting structure for magnetic particles according to claim 1, wherein the first magnetic block body is provided with a first space-saving structure on the side close to the pipe body, and the second magnetic block body is provided with a first space close to the side of the pipe body. 2. Evacuation structure. 4 . The collecting structure for magnetic particles according to claim 3 , wherein the first void avoidance structure is a first chamfered structure, a first rounded corner structure or a first concave structure. 5 . 5 . The collecting structure for magnetic particles according to claim 4 , wherein the second void avoidance structure is a second chamfered structure, a second rounded corner structure or a second concave structure. 6 . 6. The collection structure for magnetic particles according to claim 1, further comprising a clamp structure and a moving mechanism for driving the clamp structure to move, the clamp structure comprising a magnet mounting portion, the moving mechanism comprising a driving mechanism, a rack provided with guide rails and a movable mechanism. The slider slides on the guide rail, the slider is connected with the driving mechanism through the screw rod, the slider is connected with the magnet mounting part, and the magnet mounting part is provided with a first positioning cavity for placing the first magnetic block and a second magnetic The second positioning cavity of the block.

Images