CN1116088C - Simulated moving bed chromatographic separation process - Google Patents
Simulated moving bed chromatographic separation process Download PDFInfo
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- CN1116088C CN1116088C CN94109531A CN94109531A CN1116088C CN 1116088 C CN1116088 C CN 1116088C CN 94109531 A CN94109531 A CN 94109531A CN 94109531 A CN94109531 A CN 94109531A CN 1116088 C CN1116088 C CN 1116088C
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- 229920002401 polyacrylamide Polymers 0.000 description 1
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Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
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Abstract
在模拟移动床色谱分离方法中，用超临界流体作为洗脱剂，因而分离效率和操作明显改善，而且无需色谱分离后的浓缩步骤。In the simulated moving bed chromatographic separation method, the supercritical fluid is used as the eluent, so the separation efficiency and operation are obviously improved, and the concentration step after the chromatographic separation is not required.
Description
The present invention relates to a kind of simulated moving bed chromatographic separation process.Particularly, the present invention relates to a kind of new simulated moving bed chromatographic separation process, the theoretical cam curve of unit post significantly increases in this method, separates and operating efficiency height and do not need follow-up concentration step.
Chromatography separating method is in the industrial means of separating specific composition from the liquid storage that contains multiple material such as heterogeneous mixture that have been widely used as.Chromatography separating method comprises batch process and simulated moving bed process.Under two kinds of situations, sorbent used is ion exchange resin, zeolite, silica gel etc., and used eluant, eluent is water and organic solvent.In chromatographic isolation, when liquid storage with a kind of eluant, eluent during by the post that adsorbent is housed, specific composition in the liquid storage separates with other composition, and this is because adsorbent to their adsorptivity difference, therefore obtains a kind of specific composition solution and another kind of solution that does not contain said composition of containing.Can contain specific composition solution and it is concentrated by recovery and obtain this specific composition.
Yet the diffusion rate of eluant, eluent is limited in the unit post, therefore, and for the theoretical cam curve of improving separating effect increase unit post is difficult.Owing to target components and concentration in the solution after separating is low, so just need carry out concentration simultaneously, and need a storage equipment that is used for depositing weak solution.From the industrial production viewpoint see weak solution concentrated be poor efficiency and fall behind.That is to say, for extensive separation process, need large number quipments and need integrated mill.
The present invention will provide a kind of improved simulated moving bed chromatographic separation process, has overcome above-mentioned defective by the theoretical cam curve that increases the unit post, and this method is separated and operating efficiency is high and do not need follow-up concentration step.
For addressing the above problem, through simulated moving bed chromatographic separation process is deeply and carefully studied, we find, for for separating the specific purpose material the storing that contains multiple material, if in analog stream movable bed chromatographic isolation, use supercritical fluid, to increase the theoretical cam curve of unit post, thereby separation is more effective, and can saves the concentration operation step.
The invention provides a kind of SMBC method that is used for separating mixture, this method comprises: form a closed circuit of being made up of a plurality of posts, each post has an import and an outlet, and solid absorbent is housed, and said each post series connection and annular connect; To import through first module column inlet (first inlet) as the supercritical fluid of eluant, eluent and cycle through the loop to impel; With said fluid make be adsorbed on the said post and thereafter several posts on the material desorption; Emit through last outlet (first outlet) of these posts and to be rich on these posts absorption and the solution of the material of desorption (extract) from it; To contain the liquid storage of the multiple material that will separate and import next post, target substance is adsorbed on the adsorbent in said post and the several posts thereafter through the inlet of next post (second inlet); The solution that is rich in other material (raffinate) that does not adsorb is emitted in outlet (second outlet) through last post of these posts on these posts; Allow remaining solution and supercritical fluid by thereafter several pillars and be recycled to first post; The first work inlet, first exit from do, the second work inlet and second exit from do switch by post continuously with predetermined time interval on fluid flow direction successively, therefore strong adsorbent are separated with weak adsorbent.
The present invention also provides a kind of aforesaid simulated moving bed chromatographic separation process, and wherein solid absorbent is an optically-active disassemble filler.
The present invention also provides a kind of aforesaid simulated moving bed chromatographic separation process in addition, and wherein solid absorbent is selected from cellulose esters derivative particles and cellulose carbamate derivative particles and is carried on these materials on the carrier.
It is optical isomerism mixture, non-enantiomer mixture or constitutional isomer mixture that the present invention also provides the wherein separated mixture of a kind of aforesaid simulated moving bed chromatographic separation process in addition.
The present invention also provide in addition a kind of aforesaid simulated moving bed chromatographic separation process wherein in all posts a per quart pillar be used to adsorption step, purification step, desorption and eluant, eluent recycling step.
The sum of post is at least four in theory.Therefore, term " a series of " comprises zero, but is generally 8 or multicolumn more.Usually the number of post is an even number, and distributes to each step with the equal amount post.
With reference now to accompanying drawing, invention is described.
Fig. 1 is the exemplary apparatus schematic diagram that is used for simulated moving bed chromatographic separation process of the present invention.
Fig. 2 is the rough schematic of the embodiment of 12 unit posts of a kind of employing of simulated moving bed chromatographic separation process of the present invention.
In the embodiment of following the inventive method, object is separated from the fluid (A) of the multiple material that contains desire and separate by simulation moving-bed (B).
(A) contain the liquid storage of multiple material
Be applicable to that liquid storage of the present invention does not have particular determination.The example of these solution is to contain the solution that can be used for the compound in medicine, agricultural chemicals, foodstuff, feed, the spices etc.For example as many Meads sedative, chloroquine etc. in the wiping of medicine; EPN as organophosphorus pesticide; As the monosodium glutamate of flavoring, the methyl alcohol that in spices, uses.
The mixture that also comprises the optical isomer of optically-active alcohols, ester, amine, acid amides, carboxylic acid etc.Their example is just like camphorsulfonic acid ester non-enantiomer mixture of the amino acid whose Tartaric acid ester of optically-active non-enantiomer mixture, optically active amine etc.; Geometric isomer mixture such as cis-trans-isomer, or the like.
In addition, the inventive method can be used for separating the mixture of n-hexane and cyclohexane; Mixture as the close compound of boiling points such as hydrocarbon, alcohol, aldehyde and ketone; The aqueous solution that contains glucose and fructose; Maltose and greater than the polysaccharide mixture of maltotriose; The mixture that comprises the isomerized sugar of oligosaccharides; The mixture of aliphatic acid and triglycerides etc.
Simultaneously, in " US Pharmaceutical Dictionaryof Drug Names " institute's medicine of publishing in 1980 and " " agricultural chemicals described in (1979) is included in the material that the method for the invention is suitable for The Pesticicle Mannuals.
In more than describing, the solution that contains mixture of optical isomers, non-enantiomer mixture and constitutional isomer mixture can be used as the most suitable material of the inventive method.
(B) equipment of SMBC
The SMBC equipment of Shi Yonging comprises a closed circuit in the methods of the invention, and this loop is made up of a plurality of posts that solid absorbent are housed and have an inlet, an outlet.In this loop, be provided with one first inlet, import supercritical fluid by it; Be provided with one first outlet, emitted enrichment the stream of strong adsorbent (being called extract) by it; Be provided with second inlet, the liquid storage and the supercritical fluid that will contain multiple material by it import; Be provided with one second outlet, by its weak adsorbent solution (being called raffinate) of having emitted enrichment, loop direction fluid each of series of columns of flowing through according to this.This equipment further is provided with valve gear, can move the position of first and second inlets and first and second outlets at the fixed time at interval by post.When operation, liquid storage and supercritical fluid import loop through second inlet, and raffinate and extract are emitted through first and second outlets.Add the supercritical fluid of another part through first inlet as eluant, eluent.
Provide a kind of example of SMBC equipment at Fig. 1.This equipment comprises 8 unit posts.Via line (pipeline) 13, pump, rotary valve B, an a plurality of pipeline are supplied with eluant, eluent, i.e. supercritical fluid to each post.Eluant, eluent is by circulating pump 18 and the unidirectional circulation of check-valves 7a-7h.Check-valves 7a-7h prevents the eluant, eluent reverse flow.Via line (pipeline) 15, rotary valve A and a plurality of pipeline provide liquid storage to each post.First pipeline that connects rotary valve B can be delivered to the second unit post with supercritical fluid and liquid storage with first pipeline that is connected rotary valve A (as two 8 parallel circuits in Fig. 1 bottom circuit topmost).With the same manner, second pipeline can be sent to the 3rd unit post, and the 3rd pipeline is delivered to the 4th unit post and so gone down.Extract is emitted from arbitrary unit post by operation rotary valve D and connected pump via line (pipeline) 14.Raffinate can be emitted by operation rotary valve E and connected pump via line (pipeline) 16.Remaining liquid storage and part supercritical fluid via line (pipeline) 17 recirculation.The part supercritical fluid is recycled to the first module post through rotary valve C, pump 18 and circuit (pipeline) 19.
The control rotary valve is opened the entrance and exit that need open.Particularly, for example, in this case, the first work inlet, first exit from do and be loaded on the second work inlet and second exit from do of next post switch by post with predetermined time interval on fluid flow direction.Operation to rotary valve is control automatically.Equipment shown in Figure 1 can be made by those of ordinary skills, does not need to further describe.
The number of coupled columns does not have strict restriction, only needs to consider the operation scale and carry out suitable selection from the angle of Chemical Engineering.
The internal pressure of keeping closed circuit is enough to make the eluant, eluent fluid to keep supercriticality.In fact this pressure is not less than 60atm.Preferably be not less than 70atm.Loop temperature is not less than 30 ℃ usually, preferably is not less than 40 ℃.
If pressure is lower than the above value, eluent can not keep the supercritical fluid state, so it circulates with gaseous state, and this will cause separating effect to descend.Temperature range is lower than above-mentioned value, also will reduce separating effect.
Supercritical fluid
Term " supercritical fluid " is meant in temperature and is higher than critical-temperature and pressure greater than the fluid that exists with liquid under the critical pressure.The instantiation that can be in the utility of this state has carbon dioxide, nitric oxide, ammonia, sulfur dioxide, hydrogen halides, hydrogen sulfide, methane, ethane, propane, ethene, propylene, halogenated hydrocarbons etc.
When consider explosivity, during to toxicity of people etc., carbon dioxide is most preferably.When this gas when the supercritical fluid, be not less than 31.3 ℃ and pressure in temperature and be not less than under the 79.9atm. and use, just the critical state of these gases.
In described supercritical fluid, can sneak into a small amount of solvent.
The example of spendable solvent has alcohol, for example ethanol, methyl alcohol, 2-propyl alcohol etc.; Organic acid is as acetate, propionic acid etc.; Amine is as diethylamine, MEA, triethylamine etc.; Aldehyde such as acetaldehyde etc.; Ether such as oxolane, ether etc.
Used solid absorbent is also unrestricted, as long as they can adsorb the specific composition in the liquid storage.The example of adoptable adsorbent has silica gel, ion exchange resin, zeolite; The surface is through the silica gel of chemical modification or polymer coating etc.The example of the silica of a surface modification is ODS.
Among the present invention, can use a kind of filler (adsorbent) that is used for separating optical isomers aptly.
As optically-active disassemble filler, can be with being loaded with optically-active compound on it, the silica gel of low molecular compound, protein or their derivative of optically-active disassemble ability being arranged; Perhaps a kind of solid absorbent that comprises any above-claimed cpd itself and have and do not have any carrier with particle form.
The example of above optical rotatory substance has polysaccharide ester derivant, polysaccharide carbamate derivative, polyacrylate, polyamide derivative etc.
Glycan such as above-mentioned polysaccharide ester derivant and polysaccharide carbamate derivative do not have particular determination, as long as they are optically-actives.Example has natural polysaccharide, modified natural polysaccharide, synthetic polysaccharide, oligosaccharides etc.
The concrete example of above-mentioned polysaccharide has α-1,4-glucan (amylose, aminopectin), β-1,4-glucan (cellulose), α-1,6-glucan (glucan), β-1,3-glucan (curdlan, disofilan, etc.), α-1, the 3-glucan, β-1,2-glucan (Crawn Gall polysaccharide), α-1, the 6-mannosan, β-1, the 4-mannosan, β-1,2-levulan (fructan) (levulan), β-2,6-levulan (levulan), β-1, the 4-xylan, β-1, the 3-xylan, β-1, the 4-chitosan, β-1,4-N-chitin (chitin), amylopectin, agalose, arginic acid, Deng.
Wherein, most preferably cellulose, amylose, β-1,4-xylan, β-1,4-chitosan, chitin etc.
The upper limit of the average degree of polymerization of these polysaccharide (furanose average in the pyranose that contains in each molecule) is to be no more than 2000, does not preferably exceed 500 from the viewpoint of being convenient to grasp.
The example of above-mentioned oligosaccharides has maltose, Fructus Hordei Germinatus-tetrose, maltopentaose, MALTOHAXAOASE, Fructus Hordei Germinatus seven sugar, isomaltose, eluose, paratinose, maltitol, malt-orisotol, malt-tetraisotol, hydroxyl isomaltulose, alpha-cyclodextrin, beta-schardinger dextrin-, gamma-cyclodextrin etc.
Above-mentioned polysaccharide ester derivant and polysaccharide amino methanol ester derivant are the compound of polysaccharide that at least one hydrogen atom is represented by following chemical formula in its hydroxyl or the amino group replaces:
Wherein R represents one not replace common or the heteroaromatic alkyl, or a common heteroaromatic alkyl that is replaced by following substituting group: the alkyl of one or more 1-12 of containing carbon atom; one or more alkoxyls that contain 1-12 carbon atom; one or more alkylthio groups; one or more cyano group; one or more halogen atoms; one or more acyl groups that contain 1-8 carbon atom; one or more acyloxy that contain 1-8 carbon atom; one or more hydroxyls; one or more alkoxy carbonyl groups that contain 1-12 carbon atom; one or more nitros; one or more amino; one or more dialkyl amidos that contain 1-8 carbon atom.
The example of above aryl radical has phenyl, naphthyl, phenan tholyl, anthryl, indenyl, 2,3-indanyl, furyl, sulfinyl, pyryl, benzofuranyl, benzo sulfinyl, indyl, pyridine radicals, pyrimidine radicals, quinolyl, isoquinolyl etc.Wherein most preferably phenyl, naphthyl and pyridine radicals.
The x representative contains the bivalent hydrocarbon radical of 1-4 carbon atom, can contain one or more pairs of keys and a triple bond.The example of x has methylene, 1,2-ethylidene, ethyl: ethylidene e, ethenylidene, 1,2 or 1,3-propylidene, 1,1 or 2,2 one propylidynes etc.
The replacement degree of above group (1)～(4) is not less than 30%, preferably is not less than 50%, more preferably is not less than 80%.
Above-mentioned can contain substituent polysaccharide can be by acid chloride or isocyanic acid (salt) hydroxyl or amino prepared in reaction with polysaccharide.
Above-mentioned have the low molecular compound of optically-active disassemble performance that crown ether and derivative, cyclodextrin and derivative thereof are arranged.
Above-mentioned protein and derivative thereof are various antibody proteins, α
1Acidoglycoprotein, various seralbumin, egg-albumin and their derivative etc.
In the methods of the invention, the particle of above-mentioned optically-active compound, the low molecular compound that optically-active disassemble performance is arranged, protein or their derivative can be as the filler of separating optical isomers.At this moment, the particle diameter of said optically-active compound, the low molecular compound that optically-active disassemble performance is arranged, protein or derivatives thereof is generally 1 μ m-1mm, preferred 5 μ m-300 μ m, and this depends on the size of used post and piece-rate system certainly.
The particle of said optically-active compound, the low molecular compound with optically-active disassemble performance, protein or derivatives thereof can be atresia, but porous preferably.When they were porous, the aperture was 10A-100 μ m, preferred 10A-5000A.
In the methods of the invention, can optically-active compound, low molecular compound with optically-active disassemble performance, protein or their derivative of working load on suitable carrier.
As long as can the said optically-active compound of load, the low molecular compound with optically-active disassemble performance, protein or their derivative, any organic or inorganic carrier all can use.
The example of organic carrier has polystyrene, polyacrylamide, polyacrylate etc.
The example of inorganic carrier has silica gel, aluminium oxide, magnesia, glass, kaolin, titanium oxide, silicate, diatomite etc.
Wherein, silica gel, aluminium oxide and silicate are most preferably.
These carriers can be that its surface nature is modified.
The size of these carriers is 1 μ m-1mm, and preferred 5 μ m-300 μ m.Though preferred vector is a porous, it can be an atresia.When using porous carrier, its aperture is 10 -100 μ m, preferred 100 -5000 .
The amount that is carried on the described optically-active compound on the carrier, the low molecular compound with optically-active disassemble performance, protein or their derivative is the 1-100wt% of carrier, is preferably the 5-50wt% of carrier amount.When this amount during, can not realize containing the optically-active disassemble of the liquid storage of multiple material effectively less than 1wt%.When surpassing 100wt%, can not expect technique effect corresponding to this quantity.
For the inventive method, preferably comprise the solid absorbent of graininess cellulose esters derivative, cellulose carbamate derivative or these compounds of silica gel load.
Particularly " CHIRALCEL OA ", " CHIRALCEL OB ", " CHRALCEL OC ", " CHIRAL CEL OD ", " CHIRALCEL OJ ", " CHIRALCEL OG ", " CHIRALCELOF ", " CHIRALPAK AS ", " CHIRALPAK AD ", " CROHWNPAK CR (; ) ", " CHIRLCEL CA-1 ", " CHIRALCEL OK ", " CHIRALPAK WH ", " CHIRALPAKWM ", " CHIRALPAK OT (+) ", " CHIRALPAK OP (+) ", etc., be the registration mark of Daicel chemistry Co., Ltd and sell by described company.
The average grain diameter of solid absorbent is generally 1-100 μ m with kind, the simulation moving-bed variations such as volume flow of flowing through of desire separation component, preferred 5-100 μ m.Yet the average grain diameter of wishing adsorbent is 20-100 μ m.So that make the pressure loss of analog stream movable bed keep less.In this particle size range, little in the loss of simulation moving-bed upward pressure, and this pressure loss remains on and is not more than 10kgf/cm
2On the other hand, particle diameter is big more, and the theoretical cam curve of bed is more little.Therefore, if only consider the actual theoretical cam curve that absorption reaches, the average grain diameter of said solid absorbent should be 20-5.0 μ m.
Separate with SMBC
In the methods of the invention, separating the liquid storage that contains multiple components with SMBC finishes through following steps continuously by allowing this solution circulate: adsorption step (1), purification step (2), desorption (3) and eluant, eluent recycling step (4).(1) adsorption step
Allow the liquid storage that contains multiple components contact, adsorb by force composition with solid absorbent and be adsorbed on the adsorbent, weak absorption composition reclaims as raffinate with used supercritical fluid.(2) purification step
The solid absorbent that has adsorbed strong absorption composition recycles extract with part and contacts, and keeps weak in bed absorption composition like this and is removed from solid absorbent, and this process will be explained below.(3) desorption
Adsorbed the solid absorbent of strong absorption composition and contacted with supercritical fluid as eluant, eluent, therefore strong absorption composition is got off by desorption from adsorbent, and emits as extract with supercritical fluid.(4) eluant, eluent recycling step
Basically the adsorbent that only keeps supercritical fluid contacts with a part of raffinate, and a part of supercritical fluid in adsorbent is recovered like this.
Describe (1)-(4) step in detail referring now to accompanying drawing 2
In accompanying drawing 2, in 12 unit posts (1-12) solid absorbent is housed and an inlet is arranged and an outlet, their series circulation connect, and fluid can the Continuous Flow mistake like this.At first step, be that supercritical fluid is imported into post 1 by the first inlet eluant, eluent through eluant, eluent circuit 13.Extract is emitted by first outlet through extracting liquid outlet circuit 14.Provide circuit 15 to import post 7 through the stock by the liquid storage that second inlet will contain multiple components.Emit raffinate by raffinate egress line 16 from post 9.Make eluant, eluent through pipeline 17 recirculation by pump 18.
At state shown in the accompanying drawing 2, desorb is finished at unit post 1-3, and purifying is finished at unit 4-6, and absorption is finished at post 7-9, and recovery eluant, eluent just supercritical fluid is finished at post 10-12.
When operation should be simulation moving-bed, eluant, eluent provided circuit, extracting liquid outlet circuit, liquid storage to provide circuit and raffinate egress line to pursue post with a predetermined time interval and switch by operating a plurality of valves.
That is to say that in second step, desorb is finished at post 2-4, purifying is finished at post 5-7, is adsorbed on post 8-10 and finishes, and reclaims supercritical fluid and finishes at post 11-1.In kind, the post of finishing each step is switched continuously.Therefore the separation that contains the liquid storage of multiple components is to realize continuously and effectively.
From above-mentioned simulation moving-bed extraction liquid storage of emitting, the target component content is very high, is no less than 90%.Especially, when separating optical rotatory substance, the percentage of the contained optical isomer of extract is no less than 95% or be no less than 98%.The optical purity of contained other optical isomer is identical in the raffinate.
Need not say that this equipment is not limited to shown in accompanying drawing 1 and the accompanying drawing 2, but operable any kind of.
With embodiment the present invention is specified now.Yet the present invention is not limited to this, all is fine without prejudice to any suitable modifications of the present invention in this field.
Embodiment 1
SMBC equipment comprises 8 posts, column internal diameter 1cm, and column length 250cm is equipped with the cellulose iii one (3,5 one xylyl carbamate) (" CHIPALCEL OD " that is carried on the silica gel
, Daicel chemistry Co., Ltd sells, and particle diameter: 20 μ m), it is as optically-active disassemble adsorbent, and the speed of dividing with 1.5ml/ provides trans-stilbene oxide (racemic compound concentration is 10mg/ml) and finishes chromatographic isolation under the following conditions.Each step is distributed two posts.
Supercritical fluid: 3: 0.2 (volume) mixtures of carbon dioxide/ethanol
Supercritical fluid charging rate: 50ml/ branch
Extracting liquid outlet flow velocity: 35ml/ branch
Raffinate exit velocity: 16.5ml/ branch
The post switching interval time: 2.5 minutes
Simulation moving-bed temperature: 60 ℃
Simulation moving-bed pressure: (in the back pressure of outlet): 100kgf/cm
2
Obtain (+)-anti--stilbene oxide from extracting liquid outlet with 5.1mg/ branch speed, its polarimetry purity is 96%ee, is 99%ee from the raffinate outlet with the polarimetry purity that 4.9mg/ branch speed obtains (-)-anti--stilbene oxide.
Embodiment 2
SMBC equipment comprises 8 posts, column internal diameter 1cm, column length 250cm, the cellulose iii (probenicid methyl esters) that is carried on the silica gel is housed, and (" CHIRALCEL OJ " Daicel chemistry Co., Ltd sells, particle diameter: 20 μ m), be optically-active disassemble adsorbent, provide α-trityl benzyl alcohol (racemic compound concentration is 12mg/ml) chromatographic isolation under following condition, to finish with 2.5ml/ branch speed.
Supercritical fluid: carbon dioxide/3: 0.2 (volume) mixtures of 2-propyl alcohol
Supercritical fluid charging rate: 50ml/ branch
Extract is at exit velocity: the 32ml/ branch
Raffinate is at exit velocity: the 20.5ml/ branch
The post switching interval time: 11 minutes
Simulation moving-bed temperature: 60 ℃
Simulation moving-bed pressure (in the back pressure of outlet): 100kgf/cm
2
Obtain (+) that polarimetry purity is 90.5%ee-α-trityl benzyl alcohol with the 6.3mg/ branch from extracting liquid outlet, divide (-)-α-triphen methylbenzyl alcohol that obtains polarimetry purity 99%ee from the raffinate outlet with 5.7mg/.
Among the present invention, the theoretical cam curve of post has increased.In other words, the invention provides all excellent mobile chromatography separating method of simulation of a kind of separative efficiency and operating efficiency.Because eluant, eluent is evaporated automatically and removes, thereby need not step that the solution that forms is concentrated.
Claims (3)
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| CN100444920C (en) * | 2002-12-21 | 2008-12-24 | 奥代科公司 | Simulated Moving Bed Focusing Chromatography |
| FR2904776B1 (en) * | 2006-08-08 | 2009-01-23 | Inst Francais Du Petrole | METHOD AND DEVICE FOR SEPARATING A MOBILE BED SIMUL WITH A REDUCED NUMBER OF VALVES |
| FR2907021B1 (en) * | 2006-10-16 | 2009-02-06 | Inst Francais Du Petrole | METHOD AND DEVICE FOR SEPARATING A MOBILE BED SIMULATED AT A NUMBER OF LOW DIAMETER REDUCED VALVES |
| FR2956037B1 (en) * | 2010-02-11 | 2012-02-17 | Inst Francais Du Petrole | SIMPLE MOBILE BED SEPARATION METHOD AND DEVICE HAVING TWO BED DERIVATION LINES WITH CONTROLLED SWEEP FLOWS DURING INJECTION AND RETRIEVING |
| EP2552584A4 (en) * | 2010-03-31 | 2014-06-11 | Ge Healthcare Bio Sciences Ab | PARALLEL SEPARATION SYSTEM |
| GB201111589D0 (en) * | 2011-07-06 | 2011-08-24 | Equateq Ltd | New modified process |
| GB201111591D0 (en) * | 2011-07-06 | 2011-08-24 | Equateq Ltd | Further new process |
| CN103301650A (en) * | 2012-03-12 | 2013-09-18 | 江苏汉邦科技有限公司 | Intelligent control method for simulated moving bed chromatographic apparatus |
| CN103331036B (en) * | 2013-06-21 | 2014-11-26 | 浙江大学宁波理工学院 | A Chromatographic Separation Method for Separating and Enriching Target Components from a Mixture |
| CN103961902B (en) * | 2014-03-27 | 2015-08-19 | 浙江大学宁波理工学院 | Be separated from material liquid and the separation system of simulated moving bed chromatography of concentrated target components and method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5064539A (en) * | 1988-05-17 | 1991-11-12 | Ryoka Techno Engineering & Construction Co. | Method of chromatographic separation |
| EP0471082A1 (en) * | 1990-02-23 | 1992-02-19 | Daicel Chemical Industries, Ltd. | Process for separating optical isomers |
| WO1993022022A1 (en) * | 1992-04-29 | 1993-11-11 | Institut Français Du Petrole | Method and device for fractionating a mixture in a simulated fluidized bed in the presence of a compressed gas, a supercritical fluid or a subcritical fluid |
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1994
- 1994-06-17 CN CN94109531A patent/CN1116088C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5064539A (en) * | 1988-05-17 | 1991-11-12 | Ryoka Techno Engineering & Construction Co. | Method of chromatographic separation |
| EP0471082A1 (en) * | 1990-02-23 | 1992-02-19 | Daicel Chemical Industries, Ltd. | Process for separating optical isomers |
| WO1993022022A1 (en) * | 1992-04-29 | 1993-11-11 | Institut Français Du Petrole | Method and device for fractionating a mixture in a simulated fluidized bed in the presence of a compressed gas, a supercritical fluid or a subcritical fluid |
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| CN1126628A (en) | 1996-07-17 |
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