CN111568953A - Glossy privet fruit extract for resisting diabetic nephropathy and preparation method and application thereof - Google Patents

Abstract

The invention discloses an extract of Ligustrum lucidum for resisting diabetic nephropathy. By adding rice wine and/or water to Ligustrum lucidum, steaming for 10-30 hours, taking out Ligustrum lucidum, drying at 50-80 DEG C for 3-8 hours , pulverized into coarse powder, add 30-80wt% ethanol of coarse powder for ultrasonic extraction 2-3 times, each time for 0.5-2 h, combine the filtrates, recover ethanol under reduced pressure until there is no alcohol smell to obtain extract, add water to disperse the extract, add AB-8 type macroporous resin is eluted with 5BV water and 5BV ethanol solution with a volume concentration of 50% is eluted at a flow rate of 2BV/h, the ethanol eluent is collected, the ethanol is recovered, and freeze-dried to obtain the extract of Ligustrum lucidum. After the conversion of the active components of Ligustrum lucidum, the utilization value of Ligustrum lucidum is improved, and the activity is strong, and can be developed into a medicine for treating DN or a health food, and can be made into various dosage forms, which is convenient for clinical application. And the effect of the extracted compound components is better than that of salidroside, which avoids the complicated extraction procedure of traditional extraction of salidroside, and solves the problem of limited therapeutic effect of salidroside.

Description

A kind of Ligustrum lucidum extract with anti-diabetic nephropathy and preparation method and application

technical field

The invention belongs to the field of extraction of effective components of traditional Chinese medicines, and particularly relates to an extract of Ligustrum lucidum for resisting diabetic nephropathy, a preparation method and application thereof.

Background technique

Diabetic nephropathy (DN) is a common microvascular complication of diabetes and one of the leading causes of death in diabetic patients. In recent years, the incidence of DN has been on the rise, but there is still no effective drug for the treatment of DN. Traditional Chinese medicine contains a variety of active ingredients, which can produce comprehensive therapeutic effects through multiple pathways and multiple targets, and have obvious advantages and characteristics in the prevention and treatment of chronic metabolic diseases. In-depth research on traditional Chinese medicines with definite efficacy in the treatment of DN will help to better develop new drugs for the treatment of DN.

Ligustrum lucidum is the dried and ripe fruit of Ligustrum lucidum Ait., a plant in the Oleaceae family. In traditional Chinese medicine, this medicine is used to treat internal heat and quench thirst. It is now commonly used for diabetes and its complications, and is the core drug for the treatment of DN. In the past dynasties, the processing methods for women mainly included steaming, wine steaming, and nine steaming and nine drying with wine mixed with wine, etc. Now, the methods of wine steaming and wine stewing are mainly used. The 2015 edition of "Chinese Pharmacopoeia" contains two kinds of processing specifications: Ligustrum lucidum and Ligustrum lucidum. Long-term clinical practice has proved that Ligustrum lucidum can alleviate its cool and slippery properties after being prepared with wine, and enhance the effect of nourishing liver and kidney. The main active ingredients contained in Ligustrum lucidum are iridoid glycosides, triterpenes, and phenethyl alcohol. The iridoid glycosides isolated from Ligustrum lucidum mainly include specneuzhenide, privet G13 (G13), oleonuezhenide, nuezhenidic acid, neonuezhenide etc. Triterpenoids mainly include oleanolic acid, ursolic acid, etc. Phenylethanols mainly include salidroside, hydroxytyrosol, tyrosol ( tyrosol), verbascoside (acteoside), echinacoside, etc. Ligustrum is also a common green tree and street tree. Every autumn, a large number of mature privet trees fall on the ground, dyeing the ground black, causing environmental pollution and waste of resources.

Salidroside is one of the main active components of Rhodiola rosea. According to literature reports, salidroside has antioxidant function, which can reduce the production of ROS in cells, scavenge free radicals, and increase the activity of antioxidant enzymes such as CAT and SOD. Studies have confirmed that salidroside can inhibit the proliferation of mesangial cells induced by high glucose, and can also protect the kidneys from ischemia-reperfusion injury and chronic kidney disease. Studies have shown that Rhodiola has the effect of treating or inhibiting DN. Rhodiola rosea grows in plateau areas, where resources are scarce and expensive. As a substitute for it, the traditional Chinese medicine Ligustrum lucidum contains a variety of active ingredients, including salidroside, but there are also the following problems: The content of salidroside is limited, and there are many kinds of active components in Ligustrum lucidum, and the steps to separate and obtain salidroside are complicated.

At present, there is no report on extracting and isolating the extract with anti-DN from Ligustrum lucidum as raw material. In order to make full use of the resources of Ligustrum lucidum, reduce the waste of resources and environmental pollution, it is necessary to carry out in-depth research on the active components and pharmacological effects of Ligustrum lucidum, and then expand the medicinal ways of Ligustrum lucidum, for the Provide guidance on clinical application.

SUMMARY OF THE INVENTION

Aiming at the problems of the waste of resources and the high price of Rhodiola rosea in the prior art, the present invention provides an anti-DN extract of Ligustrum lucidum and a preparation method and application. When the components are transformed, the content of salidroside is significantly increased, and the anti-DN effect is enhanced by co-acting with other transformed components.

The present invention is achieved through the following technical solutions:

A kind of Ligustrum lucidum extract for anti-diabetic nephropathy is prepared by the following method:

(1) Add rice wine and/or water to Ligustrum lucidum, steam for 10 to 30 hours, take out Ligustrum lucidum, dry at 50 to 80°C for 3 to 8 hours, and pulverize into coarse powder;

(2) ultrasonically extract the coarse powder in step (1) with 30-80wt% ethanol with 30-80 wt% ethanol, each time for 0.5-2 h, filter, combine the filtrates, and recover ethanol under reduced pressure until there is no alcohol smell to obtain extract;

(3) Add water to disperse the extract, apply AB-8 macroporous resin, elute with 5BV water and 5BV 50% ethanol solution at a flow rate of 2BV/h, collect the alcohol eluent, and recover ethanol under reduced pressure , and freeze-dried extract of Ligustrum lucidum.

Further, the Ligustrum lucidum described in the step (1) is dried Ligustrum lucidum pieces, and 10-30 g of rice wine and 50-100 g of water are added to each 100 g of Ligustrum lucidum pieces.

Further, the Ligustrum lucidum described in step (1) is placed in a stainless steel container and steamed at a temperature of 80-100°C.

Further, the drying in step (1) adopts a hot air circulating drying oven to dry.

Further, the ethanol extract described in step (2) is a 50% ethanol solution.

In the present invention, the preparation method of described anti-diabetic nephropathy Ligustrum lucidum extract comprises the following steps:

(1) Add rice wine and/or water to Ligustrum lucidum, steam for 10 to 30 hours, take out Ligustrum lucidum, dry at 50 to 80°C for 3 to 8 hours, and pulverize into coarse powder;

(2) The coarse powder in step (1) is ultrasonically extracted with 30-80% ethanol of 30-80% coarse powder for 2-3 times, each time is 0.5-2 h, filtered, the filtrate is combined, and the ethanol is recovered under reduced pressure until there is no alcohol smell to obtain extract;

(3) Add water to disperse the extract, apply AB-8 macroporous resin, elute with 5BV water and 5BV 50% ethanol solution at a flow rate of 2BV/h, collect the ethanol eluent, and recover ethanol under reduced pressure , and freeze-dried extract of Ligustrum lucidum.

In the present invention, the application of the anti-diabetic nephropathy Ligustrum lucidum extract is used to prepare anti-diabetic nephropathy medicines or health care products; the present invention makes full use of the medicinal material resources of Ligustrum lucidum, and through research on the active components of Ligustrum lucidum, The prepared Ligustrum lucidum extract with high content of active ingredients and strong anti-DN activity has a protective effect on DN rats and few adverse reactions, and is expected to be developed into a new anti-DN drug and health care product.

Further, the anti-diabetic nephropathy drug or health care product is in the dosage form of tablet, capsule, granule, pill, micropill, powder, drop pill, decoction, syrup, mixture, decoction or extract.

beneficial effect

(1) The present invention mainly uses Ligustrum lucidum as a raw material, and after the active components are converted, its active parts are extracted, which improves the available value of Ligustrum lucidum, realizes the comprehensive utilization of Ligustrum lucidum resources, and develops Ligustrum lucidum anti-DN. The refined active part of the drug has strong activity and can be developed into a new drug for treating DN or a health food, and a convenient and pharmaceutically acceptable carrier can be prepared into a drug in various dosage forms, which is convenient for clinical application.

(2) The present invention through the research and screening of the chemical components and pharmacological activities of Ligustrum lucidum, shows that the content of the active components of the Ligustrum lucidum extract obtained by the present invention is as high as more than 50%; it can significantly reduce blood sugar, urine protein, and serum creatinine in DN rats. (SCr) and blood urea nitrogen (BUN), and can adjust blood lipid disorders in DN rats, improve glomerular atrophy and sclerosis, reduce renal tubular epithelial cell fatty degeneration and inflammatory cell infiltration; and the effect of the extracted compound components is better than that of the individual components. Salidroside, further demonstrating the synergistic effect of the complex components.

(3) On the basis of making full use of the traditional Chinese medicine resources of Ligustrum lucidum, the present invention not only avoids the problem of complex extraction procedures for traditional extraction of salidroside, but also solves the problem of limited therapeutic effect of salidroside. The treatment effect of DN is better.

Description of drawings

Fig. 1 is the high performance liquid chromatogram of embodiment 1 Ligustrum lucidum extract;

Figure 2 is the high performance liquid chromatogram of the extract of Ligustrum lucidum in Comparative Example 1, 1. hydroxytyrosol 2. salidroside 3. tyrosol 4. echinacoside 5. neoprivatide 6. verbasin 7. Special privet glycoside 8. privet acid 9. privet glycoside G13 10. oleonuezhenide;

Figure 3 is the morphological map of the rat kidney in each group (×200).

Detailed ways

In order to further understand the present invention, the preferred embodiments of the present invention are described below with reference to examples, but it should be understood that these descriptions are only to further illustrate the features and advantages of the present invention, rather than limit the present invention.

Example 1

Ligustrum lucidum extract with anti-DN effect is prepared by the following method:

(1) Take 100g of dry Ligustrum lucidum pieces, add 20g of rice wine and 80g of water, stir evenly in a stainless steel container, seal it, place it in a steamer, steam it over water, steam at a temperature of 100°C, steam for 24 hours, and take out Ligustrum lucidum, dried in a hot air circulation drying oven at 80 °C for 5 hours, and then pulverized into coarse powder after drying;

(2) Coarse powder in step (1) was ultrasonically extracted 3 times with 50% ethanol (50vt%) of coarse powder, 1h each time, filtered, combined filtrate, and ethanol was recovered under reduced pressure until there was no alcohol smell to obtain extract ;

(3) The extract in step (2) is dispersed with water, applied with AB-8 macroporous resin, eluted with 5BV water and 5BV ethanol solution with a volume concentration of 50% at a flow rate of 2BV/h, and collected 50% The ethanol eluent is recovered under reduced pressure, and then freeze-dried to obtain the Ligustrum lucidum extract. The high-performance liquid chromatograms of the obtained Ligustrum lucidum extract at 280 nm and 240 nm are shown in FIG. 1 .

The weight percentages of salidroside, hydroxytyrosol, tyrosol, echinacoside, verbascoside, privatide, privatide G13 and privetate in the extract of Ligustrum lucidum were determined by high performance liquid chromatography. The content was determined, and it was found that the extract of Ligustrum lucidum contained 30.0% salidroside, 1.0% hydroxytyrosol, 2.6% tyrosol, 1.5% echinacoside, 1.0% verbascoside, 11.2% secretinoside, The contents of 4.0% privet acid, 1.7% privet glycoside G13, other components oleonuezhenide and new privet glycoside were all lower than 1.0%, and the content of controllable components had reached 53.0%. Studies have shown that the extracts of Ligustrum lucidum mainly contain salidroside and salidroside, and the content of salidroside is significantly higher than that of espressoside.

Comparative Example 1

(1) Take 100 g of dried Ligustrum lucidum pieces, pulverize them into coarse powder, add 50% ethanol for ultrasonic extraction 3 times, 1 hour each time, filter, combine the filtrates, and recover ethanol under reduced pressure until there is no alcohol smell to obtain the extract;

(2) The extract is dispersed in water, applied with AB-8 macroporous resin, eluted with 5BV water and 5BV 50% ethanol solution at a flow rate of 2BV/h, collected 50% ethanol eluent, reduced The ethanol is recovered by pressure and freeze-dried to obtain the raw Ligustrum lucidum extract. The high-performance liquid chromatograms at 280 nm and 240 nm of the obtained raw Ligustrum lucidum extract are shown in FIG. 2 .

The weight percentages of salidroside, echinacoside, special privet glycoside and neoprivet glycoside in the raw Ligustrum lucidum extract prepared in Comparative Example 1 were determined by high performance liquid chromatography. The extract contains 1.0% salidroside, 1.1% echinacoside, 28.6% special privet glycoside, 29.8% privet glycoside G13, 10.3% oleonuezhenide and 8.3% new privet glycoside, and other components hydroxyphenol The respective contents of alcohol, tyrosol, verbascoside and privet acid were all lower than 1.0%. Studies have shown that the extract of raw privet seed mainly contains iridoid glycosides, of which the total amount of special privet glycosides, privet glycosides G13, oleonuezhenide and new privet glycosides reaches 77.0%.

Study on the protective effect of Ligustrum lucidum extract on DN rats

1. Experimental method

70 SD rats were taken and fed adaptively for 1 week, 10 SD rats were randomly selected according to body weight as normal group, and the remaining 60 rats were used as model group. The normal group was fed with normal diet for 4 weeks, and the model group was fed with high-sugar and high-fat diet (formula: 18% fat, 20% sucrose, 3% cholesterol and 59% basic diet) for 4 weeks, and fasted on the 4th weekend 12h (no water was allowed), the rats in the model group were given streptozotocin 35mg/kg (dissolved in 0.1mol/L, pH4.5 sodium citrate buffer before use to prepare a 1% streptozotocin solution) ) was intraperitoneally injected for 2 consecutive days, and the rats in the normal group were intraperitoneally injected with the same volume of sodium citrate buffer. After 72 hours, blood was collected from the tail vein to measure the fasting blood glucose of the rats (fasting for 10 hours before the test). The hyperglycemia model was successfully established in rats whose blood glucose value was greater than 11.1 mmol/L. Forty rats with successful modeling were selected and randomly divided into 4 groups, namely model group, Example 1 Ligustrum lucidum extract group, Comparative Example 1 Ligustrum lucidum extract group, and salidroside group, with 10 rats in each group. Example 1 The Ligustrum lucidum extract group was given 300 mg/kg of the Ligustrum lucidum extract prepared in Example 1 (gavage administration) and normal feed, and the Comparative Example 1 Ligustrum lucidum extract group was given the Ligustrum lucidum extract 330 mg/kg (the rate of obtaining paste is different, equivalent to 300 mg/kg of the Ligustrum lucidum extract prepared in Example 1, intragastric administration) and normal feed, the salidroside group was given Rhodiola rosea Glycoside 90mg/kg (gavage administration) and normal feed, the model group was given normal feed. Dosing for 8 consecutive weeks. During the experiment, the general condition, body weight, water intake, food intake and urine output of the rats were observed. At the end of the 8th week, the fasting blood glucose of the rats in each group was detected; the rats in each group were placed in the rat metabolism cage, and the 24-hour urine was collected to determine the 24-hour urine protein content. Fasting in the evening on the day of the last administration, blood was collected from the abdominal aorta on the second day to detect the levels of serum SCr, BUN, TG, TC, HDL-C and LDL-C. After the rats were blood, the kidneys were separated, weighed, and the kidney index was calculated. Kidney index = kidney weight (g)/rat body weight (g) × 1000. Some kidneys were fixed with 10% neutral formalin and stained with HE to observe the morphological and pathological changes of kidney tissue.

2. Experimental results

(1) General observation of rats

(2) During the experiment, the rats in the normal group grew well, were active, white and shiny, and had normal diet, water and urine; the rats in the model group were lethargic, sluggish, yellowish in color, rough and undisturbed. Luster, food intake, water intake and urine volume increased significantly, body weight decreased significantly, the symptoms of "three more and one less" were obvious, and the rat urine had a sour smell, and various diabetic complications gradually appeared in the later stage of the experiment, such as neck swelling. There are recurrent ulcers at the corners of the mouth, periodontal abscess bleeding, loose teeth, and diabetic fundus lesions. With the later stage of intragastric administration, the changes of physical signs of the rats in the administration groups (Example 1 Ligustrum lucidum extract group, Comparative Example 1 Ligustrum lucidum extract group and salidroside group) improved to varying degrees, and their body weights improved to varying degrees. increased, and its mental state and coat color were also improved. With the later gavage administration, the effect of the Ligustrum lucidum extract group in Example 1 was the most significant. The results of the effect on blood glucose, 24h urine protein and renal index of DN rats are shown in Table 1. Blood sugar and blood lipids are important indicators for judging renal parenchymal damage. When the kidneys are damaged, the structure and function of the kidneys will change, and the changes in blood sugar and blood lipids will directly reflect the damage to the kidneys. The study showed that compared with the normal group, the blood sugar level of the model group was significantly higher (P<0.01), indicating that the modeling was successful. Compared with the model group, the blood sugar level of the rats in the extract group of Example 1 Ligustrum lucidum was significantly decreased (P<0.01), and the blood sugar of the rats in the salidroside group was also significantly decreased (P<0.05). The blood sugar of the rats in the extract group decreased more than that in the salidroside group, but there was no significant change in the blood sugar level of the rats in the control group 1 Ligustrum lucidum extract (P>0.05).

Compared with the normal group, the 24h urine protein quantification in the model group was significantly higher (P<0.01). Compared with the model group, the 24h urine protein quantification of the rats in Example 1 Ligustrum lucidum extract group, Comparative Example 1 Ligustrum lucidum extract group and salidroside group all decreased, and there was a significant difference (P<0.01). or P<0.05), in each treatment group, the 24h urine protein quantitative reduction in Example 1 Ligustrum lucidum extract group was the most obvious.

Compared with the normal group, the renal index of the rats in the model group was significantly increased, and renal hypertrophy appeared (P<0.01). Compared with the model group, the kidney index of the rats in Example 1 Ligustrum lucidum extract group and salidroside group was significantly reduced (P<0.01 or P<0.05), and the effect of Example 1 Ligustrum lucidum extract group was more effective. Obviously, the kidney index of the rats in Comparative Example 1 was not significantly decreased, indicating that the extract of Ligustrum lucidum in Example 1 has a certain protective effect on organ damage in DN rats.

±SD，n=10）Table 1 Effects on blood glucose, 24h urine protein and renal index in DN rats (

±SD, n=10)

(3) Effects on serum creatinine SCr and blood urea nitrogen BUN in DN rats

The results are shown in Table 2. The results showed that compared with the normal group, the values of SCr and BUN in the model group were significantly increased (P<0.01). Compared with the model group, Example 1 Ligustrum lucidum extract group, Comparative Example 1 Ligustrum lucidum extract group and salidroside group all had a tendency to reduce SCr and BUN, and Example 1 Ligustrum lucidum extract group had a tendency to reduce SCr and BUN. The decreasing trend was more obvious, and it had the strongest protective effect on renal function of DN rats.

±SD，n=10）Table 2 Effects on serum SCr and BUN of DN rats (

±SD, n=10)

(4) Effects on blood lipids in rats

The results are shown in Table 3. Kidney damage is often accompanied by hyperlipidemia. In the early stage of DN, TG is mainly elevated, and in the later stage, the levels of TG, TC and LDL-C are significantly increased, and the level of HDL-C is significantly decreased. As shown in Table 3, compared with the normal group, the serum levels of LDL-C, TC and TG in the model group were significantly increased, and there was a significant difference (P < 0.01), and the level of HDL-C was significantly decreased, with a significant Sex difference (P<0.01). Compared with the model group, the levels of LDL-C, TC, and TG of the rats in the Ligustrum lucidum extract group and the salidroside group in Example 1 were significantly decreased (P<0.01 or P<0.05), and the level of HDL-C was increased (P<0.01 or P<0.05). P < 0.01); in the control group 1, the levels of TC and TG were significantly decreased (P < 0.05), the level of HDL-C was increased (P < 0.05), and there was no significant difference in the level of LDL-C. It is shown that the effect of regulating blood lipids is more significant with the extract group of Ligustrum lucidum in Example 1.

±SD）Table 3 Effects of Ligustrum lucidum extract on blood lipids in rats with diabetic nephropathy (

±SD)

(5) Effects on the morphology and pathology of rat kidneys

Figure 3 shows the morphology of the kidney tissue of the rats in each group. Observed under light microscope, the structure of the glomerulus in the normal group was normal and there was no congestion and atrophy, no degeneration and necrosis of the renal tubular epithelium, the structure of the renal tubule and interstitium was clear, and there was no hemorrhage and inflammation in the renal interstitium. Calcification, atrophy, local fibrosis, inflammatory cell infiltration, partial degeneration of renal tubular epithelium, and interstitial congestion were observed in the glomerulus of the rats in the model group, indicating that the kidneys of the rats in the model group were damaged and the modeling was successful. Example 1 The rats in the Ligustrum lucidum extract group basically returned to normal, and there was no substantial damage to the kidneys. Comparative Example 1 In the L. lucidum extract group, a small part of the glomerulus was atrophied, and the renal tubular epithelium was basically normal, with small focal inflammation in the interstitium. In the salidroside group, the glomerulus was slightly congested, a small part of the renal tubules appeared vacuolar degeneration, and the renal tubule epithelium was basically normal. Compared with the model group, there was a certain degree of improvement. The results showed that compared with the model group, the kidney damage of the rats in Example 1 Ligustrum lucidum extract group, Comparative Example 1 Ligustrum lucidum extract group and salidroside group improved to varying degrees, especially in Example 1. Ligustrum lucidum extract group was the most obvious.

Through the analysis of the experimental effects, we can draw the following conclusions: both the natural and the processed Ligustrum lucidum have protective effects on DN rats, and the processed Ligustrum lucidum is more effective than the natural Ligustrum lucidum. Comparing the processed Ligustrum lucidum extract group and the salidroside group, it was found that the protective effect of the processed Ligustrum lucidum extract group on DN rats was significantly better than that of the salidroside group. The other components of the Radix Fructus also play a certain protective effect on DN, and further illustrate that the active ingredient of the patent Ligustrum lucidum extract anti-DN is the effect of multiple active components in the Ligustrum lucidum extract working together; In terms of treatment, the patented active ingredient conversion and extraction method has also been proved to be the most effective method.

The invention makes full use of the medicinal material resources of Ligustrum lucidum, through researching the active components of Ligustrum lucidum, and prepares the extract of Ligustrum lucidum with high active component content and strong anti-DN activity, which has a protective effect on DN rats and has adverse reactions. It is expected to be developed into new anti-DN drugs and health care products.

Claims (8)

1. A glossy privet fruit extract for resisting diabetic nephropathy is characterized by being prepared by the following method:

(1) adding yellow wine and/or water into glossy privet fruit, steaming for 10-30 h in a water-proof manner, taking out the glossy privet fruit, drying for 3-8 h at 50-80 ℃, and crushing into coarse powder;

(2) adding ethanol with the amount of 30-80 wt% of the coarse powder into the coarse powder in the step (1), performing ultrasonic extraction for 2-3 times, each time for 0.5-2 hours, filtering, combining filtrates, and recovering ethanol under reduced pressure until no ethanol smell exists, thereby obtaining an extract;

(3) dispersing the extract with water, loading onto AB-8 type macroporous resin, sequentially eluting with 5BV water and 5BV 50% ethanol solution at flow rate of 2BV/h, collecting ethanol eluate, recovering ethanol under reduced pressure, and freeze drying to obtain fructus Ligustri Lucidi extract.

2. The fructus ligustri lucidi extract of claim 1, wherein the fructus ligustri lucidi in step (1) is dry fructus ligustri lucidi decoction pieces, 10-30 g yellow wine and 50-100 g water are added to each 100g fructus ligustri lucidi decoction pieces.

3. The fructus Ligustri Lucidi extract as claimed in claim 1, wherein the fructus Ligustri Lucidi in step (1) is placed in a stainless steel container and steamed in water, and the steaming temperature is 80-100 ℃.

4. The fructus ligustri lucidi extract as claimed in claim 1, wherein the drying in step (1) is performed by a hot air circulation drying oven.

5. The fructus Ligustri Lucidi extract as claimed in claim 1, wherein the ethanol extract in step (2) is 50% ethanol solution.

6. A method for preparing the extract of fructus Ligustri Lucidi for treating diabetic nephropathy according to any one of claims 1 to 5, comprising the steps of:

(1) adding yellow wine and/or water into glossy privet fruit, steaming for 10-30 h in a water-proof manner, taking out the glossy privet fruit, drying for 3-8 h at 50-80 ℃, and crushing into coarse powder;

(2) adding ethanol with the amount of 30-80% of the coarse powder into the coarse powder in the step (1), performing ultrasonic extraction for 2-3 times, each time for 0.5-2 hours, filtering, combining filtrates, and recovering ethanol under reduced pressure until no ethanol smell exists, thereby obtaining an extract;

(3) dispersing the extract with water, loading onto AB-8 type macroporous resin, sequentially eluting with 5BV water and 5BV 50% ethanol solution at flow rate of 2BV/h, collecting ethanol eluate, recovering ethanol under reduced pressure, and freeze drying to obtain fructus Ligustri Lucidi extract.

7. The application of the glossy privet fruit extract for resisting diabetic nephropathy in any one of claims 1-5, which is used for preparing a medicament or health product for resisting diabetic nephropathy.

8. The use of claim 7, wherein the anti-diabetic nephropathy pharmaceutical or health product is in the form of tablets, capsules, granules, pills, pellets, powder, dripping pills, decoction, syrup, mixture, soft extract or extract.

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