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CN111479818A - 合成寡核苷酸的方法及系统 - Google Patents

合成寡核苷酸的方法及系统 Download PDF

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Publication number
CN111479818A
CN111479818A CN201980006472.9A CN201980006472A CN111479818A CN 111479818 A CN111479818 A CN 111479818A CN 201980006472 A CN201980006472 A CN 201980006472A CN 111479818 A CN111479818 A CN 111479818A
Authority
CN
China
Prior art keywords
synthesis
nucleic acids
synthesizing oligonucleotides
synthesizing
polybasic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201980006472.9A
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English (en)
Inventor
王勇
陈世宏
周超
孙宝策
冯利鹤
黄小罗
沈玥
李汉东
章文蔚
徐讯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BGI Shenzhen Co Ltd
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BGI Shenzhen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BGI Shenzhen Co Ltd filed Critical BGI Shenzhen Co Ltd
Publication of CN111479818A publication Critical patent/CN111479818A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

提供“合成池”合成核酸的方法及系统。所述方法基于亚磷酰胺法四步循环固相合成核酸，其中脱保护、偶联、盖帽、氧化在彼此独立的第一反应容器、第二反应容器、第三反应容器、第四反应容器中进行。所述方法利用多碱基核苷酸作为核酸合成单体能够快速地合成链长更长的核酸，合成的错误率低，且试剂可以重复使用，合成效率大幅提高。

Description

PCT国内申请，说明书已公开。

Claims (39)

  1. PCT国内申请，权利要求书已公开。
CN201980006472.9A 2018-02-13 2019-02-02 合成寡核苷酸的方法及系统 Pending CN111479818A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2018101494384 2018-02-13
CN201810149438 2018-02-13
PCT/CN2019/074588 WO2019158007A1 (zh) 2018-02-13 2019-02-02 合成寡核苷酸的方法及系统

Publications (1)

Publication Number Publication Date
CN111479818A true CN111479818A (zh) 2020-07-31

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ID=67619658

Family Applications (1)

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CN201980006472.9A Pending CN111479818A (zh) 2018-02-13 2019-02-02 合成寡核苷酸的方法及系统

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CN (1) CN111479818A (zh)
WO (1) WO2019158007A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113041967A (zh) * 2021-02-26 2021-06-29 通用生物系统（安徽）有限公司 一种超长链核酸合成生产工艺

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021110079A1 (en) * 2019-12-03 2021-06-10 Bgi Shenzhen Co., Ltd. Immersion synthesis of oligonucleotides
CN111704644B (zh) * 2020-08-18 2020-12-04 苏州金唯智生物科技有限公司 一种氨解液及氨解方法
CN116903690A (zh) * 2023-07-14 2023-10-20 中国人民解放军军事科学院军事医学研究院 一种核苷二聚体及其制备方法和在dna合成中的应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1190973A (zh) * 1995-06-07 1998-08-19 拉卓拉药物公司 寡核苷酸合成的改进方法
CN1471537A (zh) * 2000-09-07 2004-01-28 ƽ 用于低聚核苷酸合成的合成子
CN1549820A (zh) * 2001-07-03 2004-11-24 ƽ 用于寡核苷酸合成的活化剂
WO2007033176A2 (en) * 2005-09-14 2007-03-22 Illumina, Inc. Continuous polymer synthesizer
CN101133073A (zh) * 2005-03-04 2008-02-27 集润德斯股份公司 寡核苷酸的合成
CN102272363A (zh) * 2008-11-06 2011-12-07 新加坡科技研究局 生物聚合物合成装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1190973A (zh) * 1995-06-07 1998-08-19 拉卓拉药物公司 寡核苷酸合成的改进方法
CN1471537A (zh) * 2000-09-07 2004-01-28 ƽ 用于低聚核苷酸合成的合成子
CN1549820A (zh) * 2001-07-03 2004-11-24 ƽ 用于寡核苷酸合成的活化剂
CN101133073A (zh) * 2005-03-04 2008-02-27 集润德斯股份公司 寡核苷酸的合成
WO2007033176A2 (en) * 2005-09-14 2007-03-22 Illumina, Inc. Continuous polymer synthesizer
CN102272363A (zh) * 2008-11-06 2011-12-07 新加坡科技研究局 生物聚合物合成装置

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
G. KUMAR AND M. S. POONIAN: "Improvements in Oligodeoxyribonucleotide Synthesis: Methyl N,N-Dialkylphosphoramidite Dimer Units for Solid Support Phosphite Methodology", 《J. ORG. CHEM.》 *
J.L. FOURREY AND D.J. SHIRE: "NEW INTERMEDIATES FOR THE SYNTHESIS OF OLIGONUCLEOTIDES BY THE PHOSPHITE TRIESTER APPROACH", 《TETRAHEDRON LETTERS》 *
M. D. MATTEUCCI AND M. H. CARUTHERS: "Synthesis of Deoxyoligonucleotides on a Polymer Support", 《J. AM. CHEM. SOC.》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113041967A (zh) * 2021-02-26 2021-06-29 通用生物系统（安徽）有限公司 一种超长链核酸合成生产工艺

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Application publication date: 20200731