CN111454839A - A novel coronavirus nucleic acid preparation and PCR detection integrated device - Google Patents

Abstract

The present application relates to a novel coronavirus nucleic acid preparation and PCR detection integrated device, comprising: a housing; a sample processing module for drawing samples from the sample stage; a nucleic acid extraction module for extracting nucleic acid; a nucleic acid transfer module, including a Three conveyor belts and a nucleic acid extraction gun, the nucleic acid extraction gun extracts nucleic acid from the nucleic acid extraction plate; PCR detection module, including PCR tube, temperature control bottom plate, laser emitter, fluorescent signal detection device, data processing and control elements and data output panel; after the nucleic acid extraction gun adds the extracted nucleic acid into the PCR tube, the laser transmitter emits laser light to irradiate the PCR tube, and the fluorescent signal detection device detects the fluorescent signal in the PCR tube, so the The data processing and control element receives the detection signal of the fluorescence signal detection device and displays the analysis result on the data output panel.

Description

A novel coronavirus nucleic acid preparation and PCR detection integrated device

technical field

The invention relates to the technical field of molecular detection, in particular to a novel coronavirus nucleic acid preparation and PCR detection integrated device.

Background technique

The statements in this section merely provide background information related to the present disclosure and do not necessarily constitute prior art.

The new coronavirus (severe acute respiratory syndrome coronavirus 2, 2019-nCoV, severe acute respiratory syndrome coronavirus 2) is particularly contagious, and nucleic acid testing needs to be carried out in the P3 laboratory, and operators need to wear protective clothing. Isolation gowns, goggles, etc. enter the P3 laboratory for extraction, and then the nucleic acid is spotted into the PCR system for real-time fluorescence quantitative PCR detection. It is extremely inconvenient and time-consuming to wear protective clothing when extracting nucleic acids. During the operation, operators are prone to hypoxia, and long-term operation is prone to fatigue, which increases the probability of virus infection. However, real-time quantitative PCR requires a 2-hour process, and requires centralized samples to be loaded on the machine together. It cannot be used for scattered samples, and it cannot be used for emergency projects. All these make the detection of the new coronavirus unable to be carried out in a timely and convenient manner.

SUMMARY OF THE INVENTION

In view of the above, it is necessary to provide an integrated device for nucleic acid preparation and PCR detection of novel coronavirus, which integrates the functions of nucleic acid preparation and PCR detection of novel coronavirus.

A novel coronavirus nucleic acid preparation and PCR detection integrated device, comprising:

case;

A sample processing module includes a sniffer gun, a first conveyor belt, and a sample stage, the sample stage being connected to the first conveyor belt to transport the sample stage; the sniffer gun is movably connected to the housing and located at the upper part of the sample stage, for drawing samples from the sample stage;

The nucleic acid extraction module includes a first telescopic rod, a second telescopic rod, an extraction magnetic frame, a nucleic acid extraction plate and a second transmission belt, and the first telescopic rod is connected to the magnetic rod of the extraction magnetic frame to drive the magnetic rod Move in the vertical direction; the second telescopic rod is connected to the extraction magnetic frame to drive the extraction magnetic frame to move in the horizontal direction, the extraction magnetic frame is provided with a small upper and a large through hole, and the extraction magnetic frame It also includes a suction head, the suction head is connected to a plastic sleeve, and the suction head is magnetically adsorbed on the bottom of the through hole; the nucleic acid extraction plate is connected to the second transfer belt, the second transfer belt moving in the horizontal direction to drive the nucleic acid extraction plate to move in the horizontal direction;

The nucleic acid transfer module includes a third conveyor belt and a nucleic acid extraction gun, the nucleic acid extraction gun expands and contracts in a vertical direction to extract nucleic acid from the nucleic acid extraction plate, and the third conveyor belt is connected to the nucleic acid extraction gun to moving the nucleic acid extraction gun horizontally;

The PCR detection module includes a PCR tube, a temperature control base plate, a laser emitter, a fluorescent signal detection device, a data processing and control element, and a data output panel. The PCR tube is set on the temperature control base plate, and the laser emitter faces the The PCR tube is irradiated with laser light to the PCR tube, the fluorescent signal detection device is located above the PCR tube and the fluorescent signal detection device is connected to the data processing control element, and the data processing control element is connected to the Data output panel; after the nucleic acid extraction gun adds the extracted nucleic acid into the PCR tube, the laser transmitter emits laser light to irradiate the PCR tube, and the fluorescence signal detection device detects the fluorescence signal in the PCR tube, The data processing and control element receives the detection signal of the fluorescence signal detection device and displays the analysis result on the data output panel.

Preferably, the housing includes a main processing chamber and a preprocessing chamber, the nucleic acid extraction module is located in the main processing chamber, one end of the first conveyor belt is located in the preprocessing chamber, and the other end is located in the main processing chamber and located below the sniffer gun.

Preferably, the housing is provided with a PCR processing chamber, the PCR processing chamber is provided with a normally closed door, the PCR detection module is provided in the PCR processing chamber, and the nucleic acid extraction gun passes through the nucleic acid extraction gun after extracting nucleic acid. The nucleic acid was added to the PCR tube with the door normally closed.

Preferably, a waste board processing chamber is further provided in the casing, one end of the third conveyor belt is located in the main processing chamber and below the extraction magnetic frame, and the other end extends into the waste board processing chamber to drive the The nucleic acid extraction plate enters the waste plate processing chamber.

Preferably, it also includes a telescopic table and a cover plate, the telescopic table is telescopic in a vertical direction and connected to the cover plate, the cover plate is located above the second conveyor belt, and follows the nucleic acid extraction plate. When the second conveyor belt enters the waste plate processing chamber, the telescopic stage drives the cover plate to fasten the nucleic acid extraction plate to seal the nucleic acid extraction plate.

Preferably, one or more ultraviolet disinfection tubes are provided in the main processing chamber, the waste plate processing chamber and the PCR processing chamber.

Preferably, the nucleic acid extraction plate comprises a first extraction box, a second extraction box and a third extraction box, the first extraction box is provided with magnetic beads, the second extraction box is provided with a washing solution, and the first extraction box is provided with a washing solution. Three extraction boxes are provided with eluent.

Preferably, a pipette tip box is further provided in the housing, and a pipette tip corresponding to the nucleic acid extraction gun is arranged in the pipette tip box.

Preferably, a first collector is arranged in the pretreatment chamber, and a hypochlorous acid pine liquor is arranged in the first collector for collecting the discarded sample stage.

Preferably, the waste plate treatment chamber is provided with a second collector, and the second collector is provided with hypochlorous acid pine liquor for collecting waste nucleic acid extraction plates.

Compared with the prior art, the above-mentioned novel coronavirus nucleic acid preparation and PCR detection integrated device can integrate nucleic acid extraction and real-time fluorescence quantitative PCR. The sample is added to the nucleic acid extraction plate of the nucleic acid extraction module, and the high-purity nucleic acid is extracted after the vibration of the extraction magnetic frame, and then the nucleic acid transfer module transfers the extracted nucleic acid to the PCR tube of the pre-placed PCR detection module. The excitation generates fluorescence, and the fluorescence signal is received by the fluorescence signal detection device, analyzed by data processing and control elements, and finally output to the data output panel for display.

The novel coronavirus nucleic acid preparation and PCR detection integrated device greatly shortens the entire process of nucleic acid extraction and PCR, and the use of a closed and UV-sterilizable shell has effectively processed and sealed the treated waste, which can effectively ensure safety. Since the amplification enzyme can be amplified at a constant temperature, the samples can be extracted at any time and PCR amplification can be performed at any time, so that the operation can be carried out around the clock, and there is no need for an appointment time. It can be used for scattered samples, and it can also meet emergency needs. By replacing the probe system for the new crown nucleic acid in the PCR tube with probes for other viruses or bacteria, or there are different probes in different tubes, the same sample nucleic acid can also detect multiple viruses at the same time, which is widely used and has broad market prospects. The new coronavirus nucleic acid preparation and PCR detection integrated device can be used not only in the laboratory department of the hospital, but also in various public places that need to be tested.

Description of drawings

In order to explain the technical solutions of the embodiments of the present invention more clearly, the following briefly introduces the accompanying drawings used in the description of the embodiments. Obviously, the drawings in the following description are some embodiments of the present invention, which are of great significance to the art For those of ordinary skill, other drawings can also be obtained from these drawings without any creative effort.

Figure 1 is a schematic diagram of the appearance and structure of the integrated device for nucleic acid preparation and PCR detection of the new coronavirus.

Figure 2 is a schematic diagram of the internal structure of the integrated device for nucleic acid preparation and PCR detection of the new coronavirus.

Figure 3 is a schematic diagram of the structure of the extraction magnetic frame of the integrated device for nucleic acid preparation and PCR detection of the novel coronavirus.

Description of main component symbols

The following specific embodiments will further illustrate the present invention in conjunction with the above drawings.

Detailed ways

In order to more clearly understand the above objects, features and advantages of the present invention, the present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that the embodiments of the present application and the features of the embodiments may be combined with each other unless there is conflict. In the following description, many specific details are set forth in order to facilitate a full understanding of the present invention, and the described embodiments are only some, but not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention.

In various embodiments of the present invention, in order to facilitate the description but not limit the present invention, the term "connection" used in the patent application description and claims of the present invention is not limited to physical or mechanical connections, but may include electrical connections. connection, whether direct or indirect. "Up", "Down", "Down", "Left", "Right", etc. are only used to indicate the relative positional relationship, and when the absolute position of the described object changes, the relative positional relationship also changes accordingly.

Figure 1 is a schematic diagram of the appearance and structure of the integrated device for new coronavirus nucleic acid preparation and PCR detection, and Figure 2 is a schematic diagram of the internal structure of the integrated device for new coronavirus nucleic acid preparation and PCR detection. As shown in Figures 1 and 2, the integrated device for nucleic acid preparation and PCR detection of the novel coronavirus includes a housing 10, a sample processing module, a nucleic acid extraction module, a nucleic acid transfer module and a PCR detection module. When in use, the sample is pretreated by the sample processing module and input into the housing 10 . Then, the nucleic acid of the sample is extracted through the nucleic acid extraction module, and transferred to the PCR detection module for detection through the nucleic acid transfer module, so as to realize the functions of nucleic acid preparation and PCR detection of the new coronavirus.

The casing 10 is hollow inside, the outer wall is provided with a first window 111 and a second window 141 , and the interior is provided with a pretreatment chamber 11 , a main processing chamber 12 , a PCR processing chamber 13 and a waste plate processing chamber 14 . One or more ultraviolet disinfection tubes 15 are arranged in the main processing chamber 12 , the waste plate processing chamber 14 and the PCR processing chamber 13 . The chambers are normally isolated from each other by isolation doors. When interactive items are required, the isolation door (which can be an automatic door) is opened to realize interconnection between the chambers.

The sample processing module includes a suction gun 22 , a first conveyor belt 20 and a sample stage 21 . The sample stage 21 is connected to the first conveyor belt 20 to transport the sample stage 21, one end of the first conveyor belt 20 is located in the pre-processing chamber 11, and the other end is located in the main processing chamber 12 and is located in the below the sniffer gun 22. The sample suction gun 22 is provided in the main processing chamber 12 and is movably connected to the housing 10 and located above the sample stage 21 , and is used for sucking samples from the sample stage 21 . In addition, the pretreatment chamber 11 is provided with a first collector 112 , and the first collector 112 is provided with hypochlorous acid pine liquor for collecting the discarded sample stage 21 .

Figure 3 is a schematic structural diagram of the extraction magnetic frame of the integrated device for nucleic acid preparation and PCR detection of the novel coronavirus. As shown in Figures 2 and 3, the nucleic acid extraction module is located in the main processing chamber 12, including a first telescopic rod 31, a second The telescopic rod 32 , the extraction magnetic stand 33 , the nucleic acid extraction plate 34 and the second conveyor belt 30 . The first telescopic rod 31 is connected to the magnetic bar 311 of the extraction magnetic stand 33 to drive the magnetic bar 311 to move in the vertical direction. In this embodiment, the first telescopic rod 31 can be connected to a plurality of magnetic rods 311 through a plate body, so as to operate multiple sets of nucleic acid extraction plates 34 at the same time. The second telescopic rod 32 is connected to the extraction magnetic frame 33 to drive the extraction magnetic frame 33 to move in the horizontal direction. The extraction magnetic frame 33 is provided with a plurality of through holes 332 with small cross-sections and large areas, and the length direction of the through holes 332 is arranged along the vertical direction. The extraction magnetic stand 33 further includes a suction head 333 , the suction head 333 is sleeved on the end of the plastic sleeve 331 , and the suction head 333 is magnetically attracted to the bottom of the through hole 332 . In this way, the extraction magnetic stand 33 is connected to the plastic sleeve 331 through the suction head 333, wherein the plastic sleeve 331 is replaceable.

The nucleic acid extraction plate 34 is connected to the second conveyor belt 30. In this embodiment, multiple groups of nucleic acid extraction plates 34 along the direction perpendicular to the paper surface can be arranged on the second conveyor belt 30, so that multiple nucleic acid extraction plates 34 can be processed simultaneously. The extraction plate 34 simultaneously performs nucleic acid extraction operations. Corresponding to the magnetic bar 311 , the nucleic acid extraction plate 34 may also be provided with multiple sets of nucleic acid extraction plates 34 along the length direction of the second conveyor belt 30 to perform nucleic acid extraction operations simultaneously. The nucleic acid extraction plate 34 includes a first extraction box, three second extraction boxes and a third extraction box. The first extraction box is provided with magnetic beads, the three second extraction boxes are respectively provided with washing liquid, and the third extraction box is provided with eluent. The second conveyor belt 30 moves in the horizontal direction to drive the nucleic acid extraction plate 34 to move in the horizontal direction. The novel coronavirus nucleic acid preparation and PCR detection integrated device further includes a telescopic table and a cover plate, the telescopic table is telescopic in the vertical direction and connected to the cover plate, and the cover plate is located at the bottom of the second conveyor belt 30 . Above, when the nucleic acid extraction plate 34 follows the second conveyor belt 30 into the waste plate processing chamber 14, the telescopic stage drives the cover plate to fasten on the nucleic acid extraction plate 34 to seal the Nucleic acid extraction plate 34. The waste plate processing chamber 14 is provided with a second collector, and the second collector is provided with a hypochlorous acid pine liquid for collecting the waste nucleic acid extraction plates 34 .

The nucleic acid transfer module includes a third conveyor belt 40 and a nucleic acid extraction gun 41 that expands and contracts in a vertical direction to extract nucleic acid from the nucleic acid extraction plate 34 . The third conveyor belt 40 is connected to the nucleic acid extraction gun 41 to move the nucleic acid extraction gun 41 in a horizontal direction. One end of the third conveyor belt 40 is located in the main processing chamber 12 and below the extraction magnetic frame 33 , and the other end extends into the waste plate processing chamber 14 to drive the nucleic acid extraction plate 34 into the waste plate Board processing chamber 14 . The main processing chamber 12 is further provided with one or more tip boxes 16 , and the nucleic acid extraction gun 41 can automatically replace the tips from the tip box 16 when needed.

The PCR detection module includes a PCR tube 50 , a temperature control base plate 51 , a laser emitter 52 , a fluorescence signal detection device 53 , a data processing and control element 54 and a data output panel 55 . The PCR detection module is set in the PCR processing chamber 13, and the PCR processing chamber 13 is provided with a normally closed door. After the nucleic acid extraction gun 41 extracts nucleic acid, the nucleic acid is added to the chamber through the normally closed door. inside the PCR tube 50. The PCR tube 50 is set on the temperature control bottom plate 51 , the laser transmitter 52 faces the PCR tube 50 to irradiate laser light to the PCR tube 50 , and the fluorescent signal detection device 53 is located at the side of the PCR tube 50 . Above and the fluorescent signal detection device 53 is connected to the data processing control element, and the data processing control element is connected to the data output panel 55 .

The working process of the novel coronavirus nucleic acid preparation and PCR detection integrated device is described in detail below.

When in use, the whole specimen is put into the sample table 21 for ultraviolet disinfection, and then transferred to the bottom of the suction gun 22 by the first conveyor belt 20; Inside the nucleic acid extraction plate 34 of the magnetic beads and washing solution;

Then, the first telescopic rod 31 drives the magnetic rod 311 to move downward until it extends into the plastic sleeve 331, so that the suction head 333 is detached from the extraction magnetic frame 33 and adsorbed on the magnetic rod 311. At this time, the plastic sleeve 331 is covered It is located at the bottom of the magnetic bar 311 .

Next, the first telescopic rod 31 continues to drive the magnetic bar 311 to move downwards and extend into the first hole of the nucleic acid extraction plate 34 to absorb the magnetic beads in the first hole of the nucleic acid extraction plate 34, and then move upward until the magnetic bar 311 is detached Nucleic acid extraction plate 34. At this time, the plastic sleeve 331 is still located below the magnetic extraction frame 33 , the suction head 333 is adsorbed on the magnetic rod 331 , and the plastic sleeve 331 is sleeved on the bottom of the magnetic rod 331 .

Then, the second conveyor belt 30 moves, so that the second hole of the nucleic acid extraction plate 34 moves to the bottom of the magnetic rod 311; the first telescopic rod 31 continues to move downward until the magnetic beads adsorbed on the plastic sleeve 331 enter the nucleic acid extraction plate Wash in the second well of 34. Then enter the third hole in the same way to continue washing, and then enter the fourth hole.

Next, the first telescopic rod 31 drives the magnetic bar 311 to move upward until the suction head 333 abuts against the extraction magnetic stand 33 . Since the through-hole 332 is wide down and up and down, the suction head 333 is blocked by the extraction magnetic stand 33 and is attracted to the extraction magnetic stand 33 . When the magnetic bar 311 continues to move upward, the suction head 333 and the plastic sleeve 331 can be separated from the magnetic bar 311, and the magnetic beads fall into the fourth hole of the nucleic acid extraction plate 34 due to the loss of magnetic force.

Finally, the magnetic bar 311 is moved above the extraction magnetic stand 33 to prepare for the next nucleic acid extraction operation. When needed, the second telescopic rod 32 is retracted to replace the suction head 333 and the plastic sleeve 331 located on the extraction magnetic stand 33 . After this step, high-purity nucleic acid is obtained by elution, and the entire extraction process takes about 20 minutes.

Next, the second conveyor belt 30 drives the nucleic acid extraction plate 34 to move to a suitable position, the nucleic acid extraction gun 41 moved by the third conveyor belt 40 sucks nucleic acid from the nucleic acid extraction plate 34, and adds the pre-placed PCR tube 50 (containing the prepared PCR tube 50). In the system), there is a temperature-controlled bottom plate 51 under the PCR tube 50, and there is an enzyme for constant temperature amplification in the PCR system, and DNA amplification can be performed at 65 degrees, without denaturation annealing and extension. DNA amplification is started as long as it is added, and each sample is set to stand for 1 hour. After excitation by the laser emitter 52, fluorescence is generated. The fluorescence signal is received by the fluorescence signal detection device 53, and analyzed by the data processing and control element 54, and finally output to the data output panel 55 for display.

The above-mentioned novel coronavirus nucleic acid preparation and PCR detection integrated device can integrate nucleic acid extraction and real-time fluorescence quantitative PCR. After placing the entire specimen into the sample stage 21, the sample suction gun 22 of the sample processing module adds the sample to the nucleic acid extraction module. In the nucleic acid extraction plate 34, the nucleic acid with high purity is extracted after the vibration of the extraction magnetic frame 33, and then the nucleic acid transfer module transfers the extracted nucleic acid to the PCR tube 50 of the pre-placed PCR detection module, and is excited by the laser emitter 52. Fluorescence is generated, and the fluorescence signal is received by the fluorescence signal detection device 53 , analyzed by the data processing and control element 54 , and finally output to the data output panel 55 for display.

The novel coronavirus nucleic acid preparation and PCR detection integrated device greatly shortens the entire process of nucleic acid extraction and PCR, and the use of a closed and UV-sterilizable shell has effectively processed and sealed the treated waste, which can effectively ensure safety. Since the amplification enzyme can be amplified at a constant temperature, the samples can be extracted at any time and PCR amplification can be performed at any time, so that the operation can be carried out around the clock, and there is no need for an appointment time. It can be used for scattered samples, and it can also meet emergency needs. Replace the probe system for new crown nucleic acid in PCR tube 50 with probes for other viruses or bacteria, or there are different probes in different tubes, the same sample nucleic acid can also detect multiple viruses at the same time, which has a wide range of uses and market prospects. broad. The new coronavirus nucleic acid preparation and PCR detection integrated device can be used not only in the laboratory department of the hospital, but also in various public places that need to be tested.

In the several specific embodiments provided by the present invention, it should be understood that it is obvious to those skilled in the art that the present invention is not limited to the details of the above-mentioned exemplary embodiments, and does not depart from the spirit or essential characteristics of the present invention. In other cases, the present invention can be implemented in other specific forms. Therefore, the embodiments are to be regarded in all respects as illustrative and not restrictive, and the scope of the invention is to be defined by the appended claims rather than the foregoing description, which are therefore intended to fall within the scope of the claims. All changes within the meaning and range of the equivalents of , are included in the present invention. Any reference signs in the claims shall not be construed as limiting the involved claim. Furthermore, it is clear that the word "comprising" does not exclude other units or steps and the singular does not exclude the plural. The terms first, second, etc. are used to denote names and do not denote any particular order.

The above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the above preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced. Neither should depart from the spirit and scope of the technical solutions of the present invention.

Claims (10)

1. An integrated device for preparing and PCR detecting new coronavirus nucleic acid is characterized by comprising:

a housing;

the sample processing module comprises a sample suction gun, a first conveying belt and a sample table, and the sample table is connected to the first conveying belt to convey the sample table; the sample suction gun is movably connected with the shell and is positioned above the sample table and used for sucking a sample from the sample table;

the nucleic acid extraction module comprises a first telescopic rod, a second telescopic rod, an extraction magnetic frame, a nucleic acid extraction plate and a second conveyor belt, wherein the first telescopic rod is connected to the magnetic rod of the extraction magnetic frame so as to drive the magnetic rod to move along the vertical direction; the second telescopic rod is connected to the magnetic force extraction frame to drive the magnetic force extraction frame to move along the horizontal direction, the magnetic force extraction frame is provided with a through hole with a small upper part and a large lower part, the magnetic force extraction frame further comprises a suction head, the suction head is connected to the plastic sleeve, and the suction head is adsorbed at the bottom of the through hole through magnetic force; the nucleic acid extraction plate is connected to the second conveying belt, and the second conveying belt moves along the horizontal direction to drive the nucleic acid extraction plate to move along the horizontal direction;

a nucleic acid transfer module including a third conveyor belt and a nucleic acid extraction gun, the nucleic acid extraction gun being extended and retracted in a vertical direction to extract nucleic acids from the nucleic acid extraction plate, the third conveyor belt being connected to the nucleic acid extraction gun to move the nucleic acid extraction gun in a horizontal direction;

the PCR detection module comprises a PCR tube, a temperature control bottom plate, a laser emitter, a fluorescent signal detection device, a data processing and control element and a data output panel, wherein the PCR tube is arranged on the temperature control bottom plate, the laser emitter faces the PCR tube to irradiate laser to the PCR tube, the fluorescent signal detection device is positioned above the PCR tube, the fluorescent signal detection device is connected with the data processing and control element, and the data processing and control element is connected with the data output panel; after the nucleic acid extraction gun adds the extracted nucleic acid into the PCR tube, the laser emitter emits laser to irradiate the PCR tube, the fluorescence signal detection device detects the fluorescence signal in the PCR tube, and the data processing and control element receives the detection signal of the fluorescence signal detection device and displays the analysis result on the data output panel.

2. The integrated apparatus for preparing and PCR detecting a new coronavirus nucleic acid of claim 1, wherein the housing comprises a main processing chamber and a pre-processing chamber, the nucleic acid extraction module is located in the main processing chamber, and the first conveyor belt has one end located in the pre-processing chamber and the other end located in the main processing chamber and located under the pipette.

3. The integrated apparatus for preparing and detecting nucleic acid of new coronavirus according to claim 2, wherein the casing is provided with a PCR processing chamber, the PCR processing chamber is provided with a normally closed door, the PCR detection module is arranged in the PCR processing chamber, and after nucleic acid is extracted by the nucleic acid extraction gun, the nucleic acid is added into the PCR tube through the normally closed door.

4. The integrated apparatus for preparing nucleic acid from a novel coronavirus and detecting PCR of claim 3, wherein a waste plate processing chamber is further disposed in the housing, one end of the third conveyor belt is disposed in the main processing chamber and below the magnetic rack, and the other end of the third conveyor belt extends into the waste plate processing chamber to drive the nucleic acid extraction plate into the waste plate processing chamber.

5. The integrated apparatus for preparing nucleic acid from a novel coronavirus and detecting PCR as claimed in claim 4, further comprising a retractable stage and a cover plate, wherein the retractable stage is retractable in a vertical direction and connected to the cover plate, the cover plate is located above the second conveyor belt, and when the nucleic acid extraction plate follows the second conveyor belt and enters the waste plate treatment chamber, the retractable stage drives the cover plate to be fastened on the nucleic acid extraction plate so as to seal the nucleic acid extraction plate.

6. The integrated apparatus for preparing and PCR detecting the nucleic acid of the new coronavirus according to claim 5, wherein one or more UV disinfection tubes are disposed in the main processing chamber, the waste plate processing chamber and the PCR processing chamber.

7. The integrated apparatus for preparing nucleic acid of novel coronavirus and detecting PCR of claim 6, wherein the nucleic acid extraction plate comprises a first extraction cartridge, a second extraction cartridge and a third extraction cartridge, wherein the first extraction cartridge is provided with magnetic beads, the second extraction cartridge is provided with a washing solution, and the third extraction cartridge is provided with an eluent.

8. The integrated apparatus for preparing nucleic acid of new coronavirus and detecting PCR according to claim 7, wherein a gun head box is further arranged in the casing, and a gun head corresponding to the nucleic acid extraction gun is arranged in the gun head box.

9. The integrated apparatus for preparing and PCR detecting new coronavirus nucleic acid of claim 8, wherein a first collector is arranged in the pretreatment chamber, and a pine hypochlorite solution is arranged in the first collector and is used for collecting a waste sample table.

10. The integrated apparatus for preparing nucleic acid from a novel coronavirus and detecting PCR of claim 9, wherein the waste plate treatment chamber is provided with a second collector, and the second collector is provided with a pine hypochlorite solution for collecting the waste nucleic acid extraction plate.

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