CN111413465B - Method for determining plant total photosynthetic carbon assimilation capacity - Google Patents
Method for determining plant total photosynthetic carbon assimilation capacity Download PDFInfo
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- CN111413465B CN111413465B CN202010254339.XA CN202010254339A CN111413465B CN 111413465 B CN111413465 B CN 111413465B CN 202010254339 A CN202010254339 A CN 202010254339A CN 111413465 B CN111413465 B CN 111413465B
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 57
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 57
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 16
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000002689 soil Substances 0.000 claims abstract description 32
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 26
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 26
- 239000012528 membrane Substances 0.000 claims abstract description 18
- 238000004364 calculation method Methods 0.000 claims abstract description 8
- 238000005286 illumination Methods 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 6
- 230000008635 plant growth Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000009792 diffusion process Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 62
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
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- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Cultivation Of Plants (AREA)
Abstract
本发明涉及生态环境技术领域，尤其涉及一种测定植物总光合碳同化能力的方法，包括以下步骤：S1、培养槽的制作；S2、植株的选择；S3、植物的种植；S4、测定植物的碳含量；S5、培养土碳含量的测定；S6、综合计算，本发明可快速、便捷、精确的测定植物无机碳总同化能力，本发明通过二氧化碳过滤膜能够有效的避免培养土中碳酸根的扩散，即外界的二氧化碳不易进入到培养土内，培养土内的二氧化碳也不易扩散到外界，此时培养土内碳的消耗量基本为植物利用，据此可以方便、精准的计算出植株总光合碳同化能力。
The invention relates to the technical field of ecological environment, in particular to a method for measuring the total photosynthetic carbon assimilation ability of plants, comprising the following steps: S1, making a culture tank; S2, selecting plants; S3, planting plants; S4, measuring the Carbon content; S5, determination of carbon content in the culture soil; S6, comprehensive calculation, the present invention can quickly, conveniently and accurately measure the total assimilation capacity of plant inorganic carbon, and the present invention can effectively avoid carbon dioxide in the culture soil through the carbon dioxide filter membrane. Diffusion, that is, the carbon dioxide from the outside is not easy to enter the culture soil, and the carbon dioxide in the culture soil is not easy to diffuse to the outside. carbon assimilation capacity.
Description
Technical Field
The invention relates to the technical field of ecological environment, in particular to a method for determining the assimilation ability of total photosynthetic carbon of a plant.
Background
In recent years, numerous experiments have demonstrated that plants can not only photosynthesize using carbon dioxide in the atmosphere as a substrate, but also photosynthesize using bicarbonate ions stored in leaves from the soil as a substrate. In view of the fact that the utilization capacity of the plant to the bicarbonate ions is not negligible, the method has important effects on accurately measuring the utilization shares of the plant to different exogenous inorganic carbon sources, measuring the total photosynthetic carbon assimilation capacity including carbon dioxide and bicarbonate ion assimilation, and accurately evaluating the productivity of the plant and screening a plant variety with high productivity.
Therefore, we propose a method for determining the total photosynthetic carbon assimilation ability of plants to solve the above problems.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a method for measuring the total photosynthetic carbon assimilation capacity of a plant.
A method for determining the total photosynthetic carbon assimilation capability of a plant comprises the following steps:
s1, preparation of culture tank: selecting a culture tank with an open upper end, filling culture soil in the culture tank, measuring the initial carbon content in the culture soil, recording the initial mass of the culture soil, measuring and adding a proper amount of water, and covering a carbon dioxide filter membrane on the upper end of the culture tank;
s2, selection of plants: selecting robust plants, dividing the plants into a group A and a group B, recording the quality of each plant, recording the quality of the plant in the group A as ma, and the quality of the plant in the group B as mb, measuring the total carbon content m1 of the plant in the group A, calculating the ratio of the total carbon content of the plant to the plant quality, recording the ratio as N, and calculating the initial total carbon content of the plant in the group B as m2 according to N;
s3, planting plants: opening an opening on a carbon dioxide filter membrane at the upper end of the culture tank, planting the plants in the group B into the culture tank, simulating a sunlight environment to illuminate the plants, recording illumination time, recording the illumination time as T, and watering in time;
s4, determining the carbon content of the plant: after a period of plant growth, measuring the total carbon content of the plant, and recording the total carbon content as m 3;
s5, measuring the carbon content of the culture soil: drying the culture soil in the culture tank, measuring the carbon content in the culture soil in the culture tank after drying, and recording as b;
s6, comprehensive calculation: recording the total photosynthetic carbon assimilation capability of the plant as A according to a formula:
and calculating the total photosynthetic carbon assimilation capacity of the plant.
The calculation method of N is as follows: and N is m 1/ma.
The calculation method of m2 is as follows: m2 is mb × N.
And S3, filling plant gum between the opening of the carbon dioxide filter membrane and the plant.
In S3, the illumination time is 100h-200 h.
The invention has the beneficial effects that: the method can rapidly, conveniently and accurately measure the total assimilation ability of the inorganic carbon of the plant, and can effectively avoid the diffusion of carbonate in the culture soil through the carbon dioxide filter membrane, namely the external carbon dioxide is not easy to enter the culture soil, the carbon dioxide in the culture soil is not easy to diffuse to the outside, at the moment, the consumption of the carbon in the culture soil is almost utilized by the plant, and therefore the total photosynthetic carbon assimilation ability of the plant can be conveniently and accurately calculated.
Drawings
FIG. 1 is a schematic diagram of a simulation in an embodiment of a method for determining the total photosynthetic carbon assimilation ability of a plant according to the present invention;
in the figure: 1 culture tank, 2 culture soil, 3 carbon dioxide filter membrane, 4 plants and 5 plant gum.
Detailed Description
The invention is further illustrated by the following figures and specific examples in conjunction with the description.
Referring to fig. 1, a method for determining total photosynthetic carbon assimilation ability of a plant, comprising the steps of:
s1, preparation of culture tank: selecting a culture tank with an open upper end, filling culture soil in the culture tank, measuring the initial carbon content in the culture soil, recording the initial mass of the culture soil, measuring and adding a proper amount of water, covering a carbon dioxide filter membrane on the upper end of the culture tank, wherein the carbon dioxide filter membrane is a common filter membrane, and the aperture of the filter membrane is smaller than the diameter of carbon dioxide molecules, so that the carbon dioxide molecules can be prevented from diffusing through the membrane;
s2, selection of plants: selecting a robust plant, selecting a seedling (one of crops such as corn, wheat, soybean and the like or a tree seedling), wherein the plant height is 8cm-12cm, specifically, in the embodiment, the plant height is 10cm +/-0.5 cm, dividing the plant into a group A and a group B, recording the quality of each plant, recording the quality of the group A plant as ma, the quality of the group B plant as mb, determining the total carbon content m1 of the plant in the group A, calculating the ratio of the total carbon content of the plant to the plant quality, recording the ratio as N, calculating the initial total carbon content of the plant in the group B according to N, and recording the initial total carbon content as m 2;
s3, planting plants: opening an opening on a carbon dioxide filter membrane at the upper end of the culture tank, planting the plants in the group B into the culture tank, simulating a sunlight environment to illuminate the plants, recording illumination time, recording the illumination time as T, and watering in time;
s4, determining the carbon content of the plant: after a period of plant growth, measuring the total carbon content of the plant, and recording the total carbon content as m 3;
s5, measuring the carbon content of the culture soil: drying the culture soil in the culture tank, measuring the carbon content in the culture soil in the culture tank after drying, and recording as b;
s6, comprehensive calculation: recording the total photosynthetic carbon assimilation capability of the plant as A according to a formula:
and calculating the total photosynthetic carbon assimilation capacity of the plant.
The calculation method of N is as follows: and N is m 1/ma.
The calculation method of m2 is as follows: m2 is mb × N.
In S3, plant gum is filled between the opening of the carbon dioxide filtering membrane and the plant, the plant gum can improve the air tightness between the plant and the carbon dioxide filtering membrane, and errors are reduced.
In S3, the illumination time is 100h-200 h.
In the embodiment, the total assimilation capacity of the inorganic carbon of the plant can be rapidly, conveniently and accurately measured, the diffusion of carbonate in the culture soil can be effectively avoided through the carbon dioxide filtering membrane, namely, external carbon dioxide is not easy to enter the culture soil, the carbon dioxide in the culture soil is not easy to diffuse to the outside, at the moment, the consumption of the carbon in the culture soil is almost utilized by the plant, and therefore the total photosynthetic carbon assimilation capacity of the plant can be conveniently and accurately calculated.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. A method for determining the total photosynthetic carbon assimilation capacity of a plant is characterized by comprising the following steps of:
s1, preparation of culture tank: selecting a culture tank with an open upper end, filling culture soil in the culture tank, measuring the initial carbon content in the culture soil, recording the initial mass of the culture soil, measuring and adding a proper amount of water, and covering a carbon dioxide filter membrane on the upper end of the culture tank;
s2, selection of plants: selecting robust plants, dividing the plants into a group A and a group B, recording the quality of each plant, recording the quality of the plant in the group A as ma, and the quality of the plant in the group B as mb, measuring the total carbon content m1 of the plant in the group A, calculating the ratio of the total carbon content of the plant to the plant quality, recording the ratio as N, and calculating the initial total carbon content of the plant in the group B as m2 according to N;
s3, planting plants: opening an opening on a carbon dioxide filter membrane at the upper end of the culture tank, planting the plants in the group B into the culture tank, simulating a sunlight environment to illuminate the plants, recording illumination time, recording the illumination time as T, and watering in time;
s4, determining the carbon content of the plant: after a period of plant growth, measuring the total carbon content of the plant, and recording the total carbon content as m 3;
s5, measuring the carbon content of the culture soil: drying the culture soil in the culture tank, measuring the carbon content in the culture soil in the culture tank after drying, and recording as b;
s6, comprehensive calculation: recording the total photosynthetic carbon assimilation capability of the plant as A according to a formula:
and calculating the total photosynthetic carbon assimilation capacity of the plant.
2. The method of claim 1, wherein N is calculated by: and N is m 1/ma.
3. The method for determining total photosynthetic carbon assimilation ability of a plant according to claim 1, wherein m2 is calculated by: m2 is mb × N.
4. The method of claim 1, wherein in S3, a plant gum is filled between the opening of the carbon dioxide filter membrane and the plant.
5. The method of claim 1, wherein the illumination time of S3 is 100-200 h.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5887547A (en) * | 1997-07-03 | 1999-03-30 | Enviromentally Correct Concepts, Inc. | Method for measuring and quantifying amounts of carbon from certain greenhouse gases sequestered in grassy and herbaceous plants above and below the soil surface |
| CN102440153A (en) * | 2011-09-09 | 2012-05-09 | 中国科学院东北地理与农业生态研究所 | A pulsed 13CO2 quantitative labeling device and plant labeling method |
| CN108333311A (en) * | 2018-02-12 | 2018-07-27 | 中国科学院地球化学研究所 | A kind of method of quick obtaining plant inorganic carbon supplement reuse share |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0386578B1 (en) * | 1989-03-08 | 1994-11-30 | Decisions Investments Corp. | Closed ecological system |
| JP2005102508A (en) * | 2003-01-17 | 2005-04-21 | Yuichi Mori | Plant cultivation implement and plant cultivation method |
| US7871826B2 (en) * | 2007-09-26 | 2011-01-18 | Air Products And Chemicals, Inc. | Method for determining carbon content of a hydrocarbon-containing mixture |
| AU2010207964B2 (en) * | 2009-02-02 | 2015-04-02 | Planetary Emissions Management | System of systems for monitoring greenhouse gas fluxes |
| WO2011094328A1 (en) * | 2010-01-26 | 2011-08-04 | The Penn State Research Foundation | Method of increasing soil resource capture in a plant |
| CN104483456B (en) * | 2015-01-04 | 2016-06-29 | 中国海洋大学 | A kind of assay method of tangleweed group carbon sequestration efficiency |
| CN105067772B (en) * | 2015-08-10 | 2016-09-21 | 中国科学院地球化学研究所 | A method for measuring plant total photosynthetic carbon assimilation capacity |
| CN105594572B (en) * | 2016-01-12 | 2018-06-22 | 沈阳农业大学 | A kind of succulent quickly enhances the cultural method of coloring |
| CN105987991B (en) * | 2016-05-10 | 2018-02-13 | 中国科学院地球化学研究所 | A kind of method for determining mangrove carbon sink |
| CN110024797A (en) * | 2019-03-25 | 2019-07-19 | 济南大学 | A kind of preparation improving photosynthesis of plant ability |
-
2020
- 2020-04-02 CN CN202010254339.XA patent/CN111413465B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5887547A (en) * | 1997-07-03 | 1999-03-30 | Enviromentally Correct Concepts, Inc. | Method for measuring and quantifying amounts of carbon from certain greenhouse gases sequestered in grassy and herbaceous plants above and below the soil surface |
| CN102440153A (en) * | 2011-09-09 | 2012-05-09 | 中国科学院东北地理与农业生态研究所 | A pulsed 13CO2 quantitative labeling device and plant labeling method |
| CN108333311A (en) * | 2018-02-12 | 2018-07-27 | 中国科学院地球化学研究所 | A kind of method of quick obtaining plant inorganic carbon supplement reuse share |
Non-Patent Citations (5)
| Title |
|---|
| The physiological processes and mechanisms for superior water productivity of a popular ground cover rice production system;Sen Li 等;《Agricultural Water Management》;20180331;第201卷;第11-20页 * |
| 不同覆盖方式下旱植水稻N、P、K吸收特性研究;卓儒洞 等;《作物研究》;20121115;第26卷(第6期);第623-626页 * |
| 水体无机碳条件对常见沉水植物生长和生理的影响;张彦辉 等;《水生生物学报》;20091130;第33卷(第6期);第1020-1030页 * |
| 荒漠植物白刺新固定碳在植物-土壤系统中的分配;李新乐 等;《草业学报》;20190228;第28卷(第2期);第33-40页 * |
| 设施土壤有机氮组分及番茄产量对水氮调控的响应;吴汉卿 等;《植物营养与肥料学报》;20190531;第25卷(第5期);第805-813页 * |
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