CN111409942A

CN111409942A - Fluorescent quantitative PCR kit for detecting African swine fever virus

Info

Publication number: CN111409942A
Application number: CN202010228261.4A
Authority: CN
Inventors: 吴佳珉
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-03-27
Filing date: 2020-03-27
Publication date: 2020-07-14

Abstract

The invention discloses a fluorescent quantitative PCR kit for detecting African swine fever virus, which comprises a box cover, wherein the lower end of the box cover is hinged with a box body, a fixed seat is fixedly connected inside the box body, a fixed groove is formed in the fixed seat, the bottom of the fixed groove is fixedly connected with a supporting seat, the upper end of the supporting seat is fixedly connected with a first spring, and the upper end of the first spring is fixedly connected with a supporting pad. Through passing the test tube with the stopper, the lid is closed to the upper end joint of spacing stopper and test tube, pushes down the bracing piece for the test tube can be fixed, when the condition of rocking appears, also can not cause the harm to the test tube, and when opening the lid, ascending power of test tube can be given to first spring, thereby can make the test tube pop out. Through the dry ice box, when the reagent needs to be transported and stored, dry ice is added into the dry ice box, so that the temperature in the reagent box is not too high.

Description

Fluorescent quantitative PCR kit for detecting African swine fever virus

Technical Field

The invention relates to the technical field of biological kits, in particular to a fluorescent quantitative PCR kit for detecting African swine fever viruses.

Background

African Swine Fever Virus (ASFV), a large, complex and ancient DNA virus. Approximately 100 years ago, the African swine fever virus is firstly determined in African kenya, and 8 months and 3 days in 2018, a news office in China agricultural rural area notifies the first African swine fever epidemic situation in China, then the virus is quickly spread to most areas in China, the research and development of the African swine fever virus in China are advanced, a scientist team successfully separates the African swine fever virus epidemic strain found in domestic pig production, the three-dimensional structure of the whole particle of the African swine fever virus is firstly analyzed by adopting a method of three-dimensional reconstruction of a single particle of a cryoelectron microscope, and conditions are created for developing a novel vaccine for preventing and treating the African swine fever.

Present kit is when the storage, and the reagent of depositing in the kit is not fixed by especial, probably will lead to the collision damage of test tube to lead to reagent to flow out unable use, and also need artifical taking out the test tube one by one when using, it is more troublesome, when reagent needs transport, also not have comparatively convenient and fast's method to store reagent, thereby probably lead to the inside high temperature of kit so that reagent can't the safe handling.

Disclosure of Invention

The invention aims to provide a fluorescent quantitative PCR kit for detecting African swine fever virus, which has the advantages of fixing a test tube and being convenient to store and take, and solves the problem that reagents cannot be used due to overhigh temperature during reagent transportation.

In order to achieve the purpose, the invention provides the following technical scheme: a fluorescence quantitative PCR kit for detecting African swine fever viruses comprises a box cover, wherein the lower end of the box cover is hinged with a box body, a fixed seat is fixedly connected inside the box body, a fixed groove is formed in the fixed seat, the bottom of the fixed groove is fixedly connected with a supporting seat, the upper end of the supporting seat is fixedly connected with a first spring, the upper end of the first spring is fixedly connected with a supporting pad, a test tube is placed at the upper end of the supporting pad, and a limiting block is clamped outside the test tube;

the dry ice box is clamped on the inner side of the box cover, a hole groove is formed in the inner portion of the dry ice box, a clamping block is fixedly connected to the left end of the box cover, a metal block is fixedly connected to the upper end of the box cover, and a piston is slidably connected to the inner portion of the metal block.

Through adopting the above technical scheme, the design supporting seat, the cooperation of first spring and supporting pad, and place the test tube in the upper end of supporting pad, make overall structure have certain elasticity, and pass the test tube with the stopper, the upper end joint of spacing stopper and test tube, through closing the lid, push down the bracing piece, drive the stopper and push down, the stopper drives spacing stopper and pushes down, make the test tube can obtain fixing, when the condition of rocking appears, also can not cause the harm to the test tube, when opening the lid, an ascending power of test tube can be given to first spring, thereby can make the test tube pop out, more convenient and fast.

The invention is further provided with: the upper end fixedly connected with of stopper, the lower extreme joint of stopper has the test tube.

Through adopting above-mentioned technical scheme, design the joint of spacing stopper and test tube, can be so that the kit when the closed condition, it is more stable to the fixed of test tube.

The invention is further provided with: the lower extreme fixedly connected with bracing piece of dry ice box, the lower extreme and the stopper contact of bracing piece.

Through adopting above-mentioned technical scheme, design the bracing piece, when the lid downwardly rotating, can drive the bracing piece downstream, the bracing piece contacts with the stopper and drives the stopper downstream.

The invention is further provided with: the left end fixedly connected with draw-in groove of box body, the inside joint of draw-in groove has the fixture block.

Through adopting above-mentioned technical scheme, the joint of design fixture block and draw-in groove is fixed lid and box body, prevents lid rebound.

The invention is further provided with: the upper end fixedly connected with second spring of piston, the upper end fixedly connected with fixed lid of second spring, fixed lid and metal block fixed connection.

Through adopting above-mentioned technical scheme, design piston, second spring and fixed lid, constituted a piston structure.

The invention is further provided with: the right end of the metal block is provided with a vent hole.

Through adopting above-mentioned technical scheme, the design air vent can make gas flow out through the air vent.

Compared with the prior art, the invention has the following beneficial effects:

1. according to the test tube ejection device, the supporting seat, the first spring and the supporting pad are matched, the test tube is placed at the upper end of the supporting pad, the integral structure has certain elasticity, the limiting block penetrates through the test tube, the limiting plug is clamped with the upper end of the test tube, the supporting rod is pressed downwards by closing the box cover to drive the limiting block to press downwards, the limiting block drives the limiting plug to press downwards, the test tube can be fixed, the test tube cannot be damaged when the box cover shakes, the first spring can exert an upward force on the test tube when the box cover is opened, and therefore the test tube can be ejected out conveniently and quickly.

2. According to the invention, by designing the dry ice box, when the reagent needs to be transported and stored, dry ice is added into the dry ice box, so that the temperature in the reagent box is not too high, the reagent cannot deteriorate and cannot be used, the upper end of the box cover is provided with the metal block, the piston, the second spring and the fixing cover which are matched to form the piston structure, and the piston structure can be ventilated through the vent hole, so that the explosion caused by too high pressure in the reagent box is prevented.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a bottom view of a portion of the present invention;

FIG. 3 is an enlarged view of the portion A of FIG. 1 according to the present invention.

In the figure: 1. a box cover; 2. a box body; 3. a fixed seat; 4. fixing grooves; 5. a supporting seat; 6. a first spring; 7. a support pad; 8. a test tube; 9. a limiting block; 10. a limiting plug; 11. a dry ice box; 12. a hole groove; 13. a support bar; 14. a clamping block; 15. a card slot; 16. a metal block; 17. a piston; 18. a second spring; 19. a fixed cover; 20. and (4) a vent hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

Please refer to fig. 1, fig. 2 and fig. 3, a fluorescence quantitative PCR kit for detecting african swine fever virus, comprising a box cover 1, wherein the lower end of the box cover 1 is hinged with a box body 2, the box cover 1 can rotate upwards, a fixing seat 3 is fixedly connected inside the box body 2, a fixing groove 4 is formed inside the fixing seat 3, the bottom of the fixing groove 4 is fixedly connected with a supporting seat 5, the upper end of the supporting seat 5 is fixedly connected with a first spring 6, the upper end of the first spring 6 is fixedly connected with a supporting pad 7 which is made of soft rubber material, a test tube 8 is arranged at the upper end of the supporting pad 7, a limiting block 9 is clamped at the outer side of the test tube 8, and the test;

the inside joint of lid 1 has dry ice box 11, and dry ice box 11 is inside to be seted up hole groove 12, and the left end fixedly connected with fixture block 14 of lid 1, the upper end fixedly connected with metal block 16 of lid 1, the inside sliding connection of metal block 16 has piston 17. Through design dry ice box 11, can put into dry ice box 11 with the dry ice to can be to the interior cooling of reagent box through hole groove 12, through design metal block 16 and piston 17, when atmospheric pressure was too big, can promote piston 17 and upwards remove.

Referring to fig. 1, the upper end of the limiting block 9 is fixedly connected with a limiting plug 10, and the lower end of the limiting plug 10 is clamped with a test tube 8. Through the joint of design spacing stopper 10 and test tube 8, can be so that the kit when the closed condition, it is more stable to test tube 8's fixed.

Referring to fig. 1, a support rod 13 is fixedly connected to the lower end of the dry ice box 11, and the lower end of the support rod 13 is in contact with the limiting block 9. Through the design of the supporting rod 13, when the box cover 1 rotates downwards, the supporting rod 13 is driven to move downwards, and the supporting rod 13 contacts with the limiting block 9 to drive the limiting block 9 to move downwards.

Referring to fig. 1, a clamping groove 15 is fixedly connected to the left end of the box body 2, and a clamping block 14 is clamped inside the clamping groove 15. The box cover 1 and the box body 2 are fixed by the clamping of the clamping block 14 and the clamping groove 15, so that the box cover 1 is prevented from moving upwards.

Referring to fig. 1 and 3, the upper end of the piston 17 is fixedly connected with a second spring 18, the upper end of the second spring 18 is fixedly connected with a fixed cover 19, and the fixed cover 19 is fixedly connected with the metal block 16. By designing the piston 17, the second spring 18 and the stationary cover 19, a piston structure is formed.

Referring to fig. 1 and 3, the right end of the metal block 16 is provided with a vent hole 20. The vent holes 20 are designed to allow gas to escape through the vent holes 20.

During the use, rotate lid 1 lapse, lid 1 drives dry ice box 11 lapse, dry ice box 11 drives bracing piece 13 lapse, bracing piece 13 lapse is driven stopper 9 lapse when lapse, stopper 9 drives stopper 10 lapse, and stopper 10 and test tube 8 joint, drive test tube 8 lapse, test tube 8 drives supporting pad 7 lapse, supporting pad 7 can give a decurrent power of first spring 6, make first spring 6 be in the state of tightening, and with 14 joints of fixture block in draw-in groove 15, just so can be so that test tube 8 is in fixed state can not lead to the damage because of rocking. When needs use reagent, move draw-in groove 15 left, make fixture block 14 and draw-in groove 15 separately, the first spring 6 that is in the state of tightening can give an ascending power of supporting pad 7 for supporting pad 7 moves upwards, thereby drives the automatic upwards pop-up of test tube 8, like this can be more convenient take out reagent. When the kit needs to store and transport, dry ice is put into the dry ice box 11, then the dry ice box 11 is clamped in the box cover 1, the box cover 1 is closed to cool the interior of the kit, thereby protecting the reagent, when the air pressure in the kit is overlarge, the air has an upward force, the piston 17 is driven to move upwards, the piston 17 can be driven to be in a tight state by the second spring 18 when moving upwards, the air can be discharged through the vent hole 20, when the air pressure in the kit is normal, the piston 17 can be given a downward force by the second spring 18, the piston 17 can be returned to the original position again, so that the effect of the dry ice in the kit can not explode due to the overlarge air pressure, the transportation of the reagent is ensured under the condition of controlling the internal temperature, and the reagent box has better safety.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A fluorescence quantitative PCR kit for detecting African swine fever virus comprises a box cover (1) and is characterized in that: the lower end of the box cover (1) is hinged with a box body (2), a fixed seat (3) is fixedly connected inside the box body (2), a fixed groove (4) is formed in the fixed seat (3), a supporting seat (5) is fixedly connected to the bottom of the fixed groove (4), a first spring (6) is fixedly connected to the upper end of the supporting seat (5), a supporting pad (7) is fixedly connected to the upper end of the first spring (6), a test tube (8) is placed at the upper end of the supporting pad (7), and a limiting block (9) is clamped on the outer side of the test tube (8);

the inner side of the box cover (1) is clamped with a dry ice box (11), a hole groove (12) is formed in the dry ice box (11), a clamping block (14) is fixedly connected to the left end of the box cover (1), a metal block (16) is fixedly connected to the upper end of the box cover (1), and a piston (17) is slidably connected to the inner portion of the metal block (16).

2. The fluorescent quantitative PCR kit for detecting African swine fever virus according to claim 1, wherein: the upper end fixedly connected with of stopper (9) stopper (10), the lower extreme joint of stopper (10) has test tube (8).

3. The fluorescent quantitative PCR kit for detecting African swine fever virus according to claim 1, wherein: the lower end of the dry ice box (11) is fixedly connected with a supporting rod (13), and the lower end of the supporting rod (13) is in contact with a limiting block (9).

4. The fluorescent quantitative PCR kit for detecting African swine fever virus according to claim 1, wherein: the left end fixedly connected with draw-in groove (15) of box body (2), the inside joint of draw-in groove (15) has fixture block (14).

5. The fluorescent quantitative PCR kit for detecting African swine fever virus according to claim 1, wherein: the upper end of the piston (17) is fixedly connected with a second spring (18), the upper end of the second spring (18) is fixedly connected with a fixed cover (19), and the fixed cover (19) is fixedly connected with the metal block (16).

6. The fluorescent quantitative PCR kit for detecting African swine fever virus according to claim 1, wherein: the right end of the metal block (16) is provided with a vent hole (20).