CN111345904A - A heart fixation device for confocal imaging of small animal hearts - Google Patents

Abstract

The invention relates to a heart fixing device for confocal imaging of a small animal heart, which comprises a transparent bottom plate, a piston tube and a piston, wherein a groove body is formed in the bottom of the transparent bottom plate, the piston tube penetrates through the top of the transparent bottom plate and extends into the groove body, the piston is arranged in the piston tube, and the heart for an experiment is fixed between the piston and an experiment table. Compared with the prior art, the invention has simple structure and convenient operation, can fix the heart for experiment by manually pushing and pulling the piston, and avoids the adverse effect of the heart beating on the heart confocal imaging.

Description

A heart fixation device for confocal imaging of small animal hearts

technical field

The invention belongs to the technical field of organ imaging fixing devices, and relates to a small animal heart confocal imaging heart fixing device.

Background technique

The isolated heart is a common model for studying cardiac function, and the demand for perfusion heart imaging is increasing. In order to maintain the function of the heart, the isolated heart is in a perfusion state, and the beating heart will affect the focus of the microscope during imaging. Even if a drug that inhibits the heartbeat is added to the perfusion fluid, the myocardium will still beat to a certain extent, and the flow of the perfused fluid will also The focus of the heart is affected and a clear image cannot be obtained.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a small animal cardiac confocal imaging heart fixation device in order to overcome the above-mentioned defects in the prior art, which is used to solve the problem of unclear cardiac confocal imaging due to experimental heart beating.

The object of the present invention can be realized through the following technical solutions:

A heart fixation device for confocal imaging of small animal hearts, the heart fixation device is arranged on a laboratory table, and the heart fixation device comprises a transparent bottom plate, a piston tube and a piston;

The bottom of the transparent bottom plate is provided with a groove body;

The piston tube penetrates the top of the transparent bottom plate and extends into the tank;

The piston is arranged in the piston tube;

The experimental heart is fixed between the piston and the experimental table.

According to the size of the experimental heart, adjust the position of the piston in the piston tube by moving up and down, so as to fix the experimental heart between the piston and the experimental table, and avoid the influence of heart beat on the clarity of cardiac confocal imaging.

Further, the piston includes a piston head, a piston shaft and a piston handle which are connected in sequence from bottom to top along the axial direction of the piston tube.

The experimental heart is specifically fixed between the piston head and the experimental table, and the position of the piston head in the piston tube can be adjusted by manually pushing and pulling the piston handle, so as to adapt to the size of different experimental hearts.

Further, the piston tube is a transparent piston tube, so as to facilitate cardiac confocal imaging characterization.

Further, the piston tube is arranged in the center of the top of the transparent bottom plate.

Further, a gap of 8-15 mm is set between the lower end of the piston tube and the experimental table, which is used to provide a reclining setting of the perfused heart and a space for placing the perfusion tube.

Further, the side surface of the transparent tank body is provided with a side opening communicated with the tank body, and the side opening is used as the inlet and outlet of the cardiac perfusion capillary.

Further, the side openings extend to the top and bottom of the transparent bottom plate, so as to facilitate the angle adjustment of the cardiac perfusion capillary and the construction of the test device.

Further, the cross section of the side opening is in a "V" shape.

Working principle: According to the size of the experimental heart, the present invention adjusts the position where the bottom of the piston head extends out of the piston tube by manually pushing and pulling the piston handle to adapt to the size of different experimental hearts, thereby fixing the experimental heart between the piston head and the experimental bench. time, and avoid the impact of heart beat on the resolution of cardiac confocal imaging.

Compared with the prior art, the present invention has the following characteristics:

1) The present invention is simple in structure and convenient in operation, and the experimental heart can be fixed by manually pushing and pulling the piston, and the adverse effect of heart beat on cardiac confocal imaging can be avoided;

2) The stepless adjustment of the fixed height can be realized by moving the piston, so as to adapt to the isolated hearts of different sizes.

Description of drawings

1 is a schematic structural diagram of a small animal heart confocal imaging heart fixation device in the present invention;

Description of marks in the figure:

1-transparent bottom plate, 2-piston tube, 3-piston, 301-piston head, 302-piston shaft, 303-piston handle, 4-opening.

Detailed ways

The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. This embodiment is implemented on the premise of the technical solution of the present invention, and provides a detailed implementation manner and a specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

Example 1:

A small animal heart confocal imaging heart fixation device as shown in FIG. 1 , the heart fixation device is set on a laboratory bench and includes a transparent bottom plate 1, a piston tube 2 and a piston 3;

Wherein, the bottom of the transparent base plate 1 is provided with a groove body; the piston tube 2 is a transparent piston tube, and the transparent piston tube penetrates the top center of the transparent base plate 1 and extends into the groove body, and between the lower end of the piston tube 2 and the test bench is also provided with A clearance of 12 mm; the piston 3 includes a piston head 301 , a piston shaft 302 and a piston handle 303 connected in sequence from bottom to top along the axial direction of the piston tube 2 , and the piston head 301 is arranged in the piston tube 2 .

In addition, the side surface of the transparent tank 1 is provided with a side opening 4 that communicates with the tank and has a "V"-shaped cross section. The side opening 4 extends up and down to the top and bottom of the transparent bottom plate 1 .

Working principle: According to the size of the experimental heart, the present invention adjusts the position where the bottom of the piston head 301 protrudes out of the piston tube 2 by manually pushing and pulling the piston handle 303 to adapt to the size of different experimental hearts, thereby fixing the experimental heart to the piston head 301. between the laboratory bench and to avoid the impact of heart beat on the clarity of cardiac confocal imaging.

Example 2:

In this embodiment, a gap of 15 mm is set between the lower end of the piston tube 2 and the test table, and the rest are the same as those in Embodiment 1.

Example 3:

In this embodiment, a gap of 8 mm is set between the lower end of the piston tube 2 and the test table, and the rest are the same as those in Embodiment 1.

The foregoing description of the embodiments is provided to facilitate understanding and use of the invention by those of ordinary skill in the art. It will be apparent to those skilled in the art that various modifications to these embodiments can be readily made, and the generic principles described herein can be applied to other embodiments without inventive step. Therefore, the present invention is not limited to the above-mentioned embodiments, and improvements and modifications made by those skilled in the art according to the disclosure of the present invention without departing from the scope of the present invention should all fall within the protection scope of the present invention.

Claims (8)

1. A heart fixing device for small animal confocal cardiac imaging is arranged on an experiment table and is characterized by comprising a transparent bottom plate (1), a piston tube (2) and a piston (3);

the bottom of the transparent bottom plate (1) is provided with a groove body;

the piston tube (2) penetrates through the top of the transparent bottom plate (1) and extends into the groove body;

the piston (3) is arranged in the piston pipe (2);

the experimental heart is fixed between the piston (3) and the experimental table.

2. The heart fixing device for confocal small animal cardiac imaging according to claim 1, wherein the piston (3) comprises a piston head (301), a piston shaft (302) and a piston handle (303) which are sequentially connected from bottom to top along the axial direction of the piston tube (2).

3. The heart fixation device for confocal small animal cardiac imaging according to claim 2, wherein the piston tube (2) is a transparent piston tube.

4. The heart fixation device for confocal small animal cardiac imaging according to claim 1, wherein the piston tube (2) is disposed at the center of the top of the transparent base plate (1).

5. The heart fixing device for confocal small animal cardiac imaging according to claim 1, wherein a gap of 8-15mm is provided between the lower end of the piston tube (2) and the experiment table.

6. The heart fixing device for the confocal small animal cardiac imaging according to claim 1, wherein a side opening (4) communicated with the groove body is formed in the side surface of the transparent groove body (1).

7. The heart fixation device for confocal small animal cardiac imaging according to claim 6, wherein the side openings (4) extend to the top and bottom of the transparent base plate (1).

8. The small animal confocal cardiac imaging cardiac fixation device as claimed in claim 1, wherein the lateral opening (4) has a V-shaped cross-section.

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