CN111334406A - A Novel Nucleic Acid Propulsion Extraction and Purification Instrument - Google Patents

Abstract

The invention relates to a novel nucleic acid push type extraction and purification instrument which comprises an instrument body, a power supply, an electric wire, a heating sheet, a temperature controller, a reaction hole, a reaction tube, a liquid collecting tube, a liquid conveying tube and an injection and sample adding device, wherein the power supply is connected with the temperature controller and the heating sheet, and the liquid conveying tube is connected with the injection and sample adding device and the reaction tube. The purification instrument is provided with a heating plate, and the heating plate is arranged in the reaction hole. The purification instrument can be used for extracting and purifying nucleic acid in families, remote areas, epidemic situations, fields and other occasions. The reaction reagent of the purifier is in an isolated state in the whole extraction operation process, so that the exogenous pollution and cross infection can be reduced. The reaction reagents are all configured in advance, and field manual sample adding is not needed, so that the operation procedure of nucleic acid purification is greatly simplified. In a word, the device is simple to operate and short in time, and can rapidly, independently and efficiently complete nucleic acid extraction and purification without other experimental equipment.

Description

A Novel Nucleic Acid Propulsion Extraction and Purification Instrument

technical field

The invention relates to the technical field of nucleic acid purification and extraction, in particular to a novel nucleic acid push-type extraction and purification instrument.

Background technique

Nucleic acid extraction and purification is the starting point for downstream nucleic acid detection, research or product development, and the quality and integrity of the isolated nucleic acid directly affects the results of research or diagnosis. Therefore, nucleic acid extraction is one of the key methods of molecular biology. At present, there are many specialized nucleic acid extraction methods that can extract DNA, RNA or total nucleic acid from various biological samples. Most of these methods have been developed into commercial kits that can be combined with instruments to automate extraction, thereby greatly simplifying the extraction process.

At present, the kits sold on the market can basically only carry out nucleic acid extraction and purification work in places with laboratory conditions. Most of the kits need to be used with a water bath and a centrifuge. Such testing is inconvenient to be carried out in remote areas, epidemic sites, and in the wild. Moreover, when DNA extraction is performed by traditional methods, multiple tubes will be transferred, which will lead to fewer target samples to be tested when there are few samples to be tested, which greatly affects the authenticity and accuracy of the test.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a novel nucleic acid push-type extraction and purification apparatus to solve the problems raised in the above background art.

In order to achieve the above purpose, the present invention provides the following technical solutions: a novel nucleic acid propulsion extraction and purification instrument, comprising a body, a wire, a liquid collection tube and an injection and sample addition device, a power supply is arranged under the interior of the body, and a part of the power supply is provided. A temperature controller is arranged on the side, a reaction hole is arranged on the inside of the body, and a heating sheet is fixedly connected to the body wall of the reaction hole. Inside the reaction hole, a reaction tube is arranged inside the liquid collection tube, and a filter membrane is arranged inside the reaction tube.

Preferably, a plurality of the reaction holes are provided, and the reaction holes are sequentially arranged inside the body.

Preferably, the cross section of the heating sheet is annular, and the heating sheet, the temperature controller and the power source are connected by wires.

Preferably, the liquid collection tube sleeve is outside the reaction tube, and there is a space between the liquid collection tube and the reaction tube.

Preferably, an infusion tube is connected to the lower part of the injection device, and a conduit is connected to the infusion tube, and the conduit is inserted inside the hollow plug

Preferably, the injection and sample adding device comprises a first syringe, a second syringe, a third syringe and a fourth syringe, the first syringe, the second syringe, the third syringe and the fourth syringe are arranged in sequence and connected with the infusion tube connected.

Preferably, the first syringe, the second syringe, the third syringe and the fourth syringe are respectively filled with lysis solution, cleaning solution, air and eluent (detection solution).

Compared with the prior art, the present invention has the advantages that the purifier is suitable for nucleic acid extraction and purification in homes, remote areas, epidemic sites and field situations. The purifier is provided with heating pieces, and the heating pieces are all surrounded in the reaction hole of the instrument, which can better heat the liquid in the reaction tube and the liquid collection tube, avoiding the use of a water bath and a centrifuge. At the same time, the heating temperature and time can be controlled according to the previous settings. All the reaction reagents are in an isolated state, and the setting of the air layer in the infusion tube can well seal and store all the reagents participating in the reaction, which reduces foreign contamination and cross infection. The reaction reagents are all configured in advance (lysing solution, cleaning solution, air and eluent (detection solution) are configured in the injection device), and manual sample addition is not required on site, which greatly reduces the workload of nucleic acid purification. In short, the purifier is simple to operate and takes a short time, and can quickly, independently and efficiently complete nucleic acid extraction and purification without the aid of any other experimental equipment.

Description of drawings

1 is a schematic structural diagram of a novel nucleic acid push-type extraction and purification apparatus of the present invention;

2 is a schematic diagram of the internal structure of the instrument reaction well of a novel nucleic acid push-type extraction and purification instrument of the present invention;

FIG. 3 is a schematic structural diagram of the injection and sample addition device of a novel nucleic acid propulsion extraction and purification apparatus of the present invention.

In the picture: 1. Body, 2. Power supply, 3. Temperature controller, 4. Electric wire, 5. Reaction hole, 6. Heating plate, 7. Liquid collection tube, 8. Reaction tube, 9. Filter membrane, 10. Hollow Stopper, 11, catheter, 12, infusion tube, 13, injection and sample addition device, 1301, first syringe, 1302, second syringe, 1303, third syringe, 1304, fourth syringe.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Please refer to Figure 1-3, a new type of nucleic acid propulsion extraction and purification instrument, including body 1, power supply 2, temperature controller 3, electric wire 4, reaction hole 5, heating plate 6, liquid collection tube 7, reaction tube 8, filter The membrane 9, the hollow plug 10, the conduit 11, the infusion tube 12 and the injection device 13 are characterized in that: a power supply 2 is arranged under the inside of the body 1, and a temperature controller 3 is arranged on one side of the power supply 2, so The interior of the body 1 is provided with a reaction hole 5, the body wall of the reaction hole 5 is fixedly connected with a heating sheet 6, the heating sheet 6 is connected with the temperature controller 3 through the wire 4, and the liquid collection pipe 7 is provided with Inside the reaction hole 5 , a reaction tube 8 is arranged inside the liquid collection tube 7 , and a filter membrane 9 is arranged inside the reaction tube 8 .

Further, a plurality of the reaction holes 5 are provided, and the reaction holes 5 are sequentially arranged inside the body 1 .

Further, the cross section of the heating sheet 6 is annular, and the heating sheet 6 , the temperature controller 3 and the power source 2 are connected by wires 4 .

Further, the liquid collection tube 7 is covered with the outside of the reaction tube 8 , and there is a space between the liquid collection tube 7 and the reaction tube 8 .

Further, an infusion tube 12 is connected to the lower part of the injection and sample adding device 13 , and a conduit 11 is connected to the infusion tube 12 , and the conduit 11 is inserted into the interior of the hollow plug 10

Further, the injection and sample addition device 13 includes the first syringe 1301 , the second syringe 1302 , the third syringe 1303 and the fourth syringe 1304 , the first syringe 1301 , the second syringe 1302 , the third syringe 1303 and the The fourth syringes 1304 are arranged in sequence and connected to the infusion tube 12 .

Further, the first syringe 1301, the second syringe 1302, the third syringe 1303 and the fourth syringe 1304 are respectively filled with lysing solution, cleaning solution, air, eluent (detection solution) and air.

The working principle of this embodiment: the new nucleic acid extraction and purification instrument suitable for remote areas and epidemics, when in use, the staff adds the sample to be detected in the reaction tube 8, then turns on the power supply 2, and starts the temperature controller 3 to make the heating plate 10. Start heating, heat the sample for 10 minutes, and then slowly push the first syringe 1301 in the injection device 13, so that the lysate in the first syringe 1301 enters the reaction tube 8, and slowly push the second syringe 1302, The cleaning liquid enters the reaction tube 8, and at the same time, the cracking hydraulic pressure is pushed out of the reaction tube 8, and the third syringe 1303 is slowly pushed, and the air enters the reaction tube 8. Syringe 1304, the eluent (detection solution) is injected into the reaction tube 8, and part of the eluent (detection solution) enters the replaced liquid collection tube 7, then the replaced liquid collection tube 7 is covered with a cover, and then the heating chip is activated 6. Heat the replaced liquid collection tube 7 for 30 minutes, and then perform fluorescence detection on it. That's how the device works.

Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, it is still possible to modify the technical solutions described in the foregoing embodiments, or to perform equivalent replacements for some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims (7)

1. A novel nucleic acid propulsion extraction and purification instrument, comprising a body (1), an electric wire (4), a liquid collection tube (7) and an injection device (13), characterized in that: the lower part of the interior of the body (1) A power supply (2) is provided, a temperature controller (3) is provided on one side of the power supply (2), a reaction hole (5) is provided on the inside of the body (1), and the body of the reaction hole (5) is provided A heating sheet (6) is fixedly connected on the wall, the heating sheet (6) is connected with the temperature controller (3) through a wire (4), and the liquid collecting pipe (7) is arranged inside the reaction hole (5) , a reaction tube (8) is arranged inside the liquid collecting tube (7), and a filter membrane (9) is arranged inside the reaction tube (8). 2 . The novel nucleic acid propulsion extraction and purification apparatus according to claim 1 , wherein a plurality of the reaction holes ( 5 ) are provided, and the reaction holes ( 5 ) are sequentially arranged inside the body ( 1 ). 3 . 3. A kind of novel nucleic acid push-type extraction and purification apparatus according to claim 1, is characterized in that: the cross section of described heating sheet (6) is annular, and heating sheet (6), temperature controller (3) It is connected with the power supply (2) through a wire (4). 4. A new type of nucleic acid propulsion extraction and purification apparatus according to claim 1, characterized in that: the liquid collection tube (7) is covered with the outside of the reaction tube (8), and the liquid collection tube (7) is connected to the reaction tube (8). There are spaces between the tubes (8). 5. A novel nucleic acid push-type extraction and purification apparatus according to claim 1, characterized in that: an infusion tube (12) is connected to the lower part of the injection device (13), and the infusion tube (12) is connected to the lower part. There is a conduit (11) which is inserted inside the hollow plug (10). 6. A novel nucleic acid push-type extraction and purification apparatus according to claim 1, characterized in that: the injection and sample addition device (13) comprises a first syringe (1301), a second syringe (1302), and a third syringe (1303) and the fourth syringe (1304), the first syringe (1301), the second syringe (1302), the third syringe (1303) and the fourth syringe (1304) are arranged in sequence, and are connected with the infusion tube (12) connected. 7. A novel nucleic acid push-type extraction and purification apparatus according to claim 1, characterized in that: the first syringe (1301), the second syringe (1302), the third syringe (1303) and the fourth syringe ( 1304) were filled with lysing solution, cleaning solution, air and eluent (detection solution).

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