CN111175086A - Method for manufacturing dropping bottle for metering and transferring trace liquid - Google Patents
Method for manufacturing dropping bottle for metering and transferring trace liquid Download PDFInfo
- Publication number
- CN111175086A CN111175086A CN202010103403.4A CN202010103403A CN111175086A CN 111175086 A CN111175086 A CN 111175086A CN 202010103403 A CN202010103403 A CN 202010103403A CN 111175086 A CN111175086 A CN 111175086A
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- Prior art keywords
- tip
- dropper bottle
- dropper
- bottle
- combination
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- 239000007788 liquid Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 12
- 239000002184 metal Substances 0.000 claims abstract description 31
- 229910052751 metal Inorganic materials 0.000 claims abstract description 31
- 238000010438 heat treatment Methods 0.000 claims abstract description 20
- 238000005520 cutting process Methods 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000004026 adhesive bonding Methods 0.000 claims description 3
- 238000002844 melting Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 6
- 238000003745 diagnosis Methods 0.000 description 5
- 230000005484 gravity Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000012123 point-of-care testing Methods 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F19/00—Calibrated capacity measures for fluids or fluent solid material, e.g. measuring cups
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- Life Sciences & Earth Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Fluid Mechanics (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
本发明公开了一种用于微量液体计量和移液的滴瓶制作方法，包括以下步骤：切割一次性移液枪头，获得枪头针尖，所述枪头针尖的内径大于滴瓶尖头；将所述枪头针尖套设在滴瓶尖头上，并将所述枪头针尖与滴瓶尖头密封固定，获得滴瓶组合尖头；取预定外径的金属丝，将所述金属丝穿设在滴瓶组合尖头的孔径内；将穿有金属丝的滴瓶组合尖头竖直放置，对所述穿有金属丝的滴瓶组合尖头加热，使得滴瓶组合尖头融化并与金属丝贴合，之后停止加热；将金属丝从加热后的滴瓶组合尖头中抽出；切割去除滴瓶组合尖头的下端部，获得具有目标内径的滴瓶组合尖头。其可以用于重复分配固定体积的样本或试剂，成本低，适用范围广。
The invention discloses a method for manufacturing a dropper bottle for measuring and pipetting of trace liquid, comprising the following steps: cutting a disposable pipette tip to obtain a tip of the tip, wherein the inner diameter of the tip of the tip is larger than the tip of the dropper bottle; The tip of the gun tip is sleeved on the tip of the dropper bottle, and the tip of the gun tip and the tip of the dropper bottle are sealed and fixed to obtain the tip of the dropper bottle; It is arranged in the aperture of the dropper bottle combination tip; the dropper bottle combination tip with metal wire is placed vertically, and the dropper bottle combination tip with metal wire is heated, so that the dropper bottle combination tip is melted and melted. Fit with the metal wire, and then stop heating; pull the metal wire out of the heated dropper combination tip; cut and remove the lower end of the dropper combination tip to obtain the dropper bottle combination tip with the target inner diameter. It can be used to repeatedly dispense a fixed volume of samples or reagents, with low cost and a wide range of applications.
Description
Technical Field
The invention relates to the technical field of liquid transfer, in particular to a method for manufacturing a dropping bottle for metering and transferring trace liquid.
Background
High precision liquid handling is the basis for disease diagnosis, and commercial pipettors have been widely used in the field of point-of-care (POC) diagnostics. Pipetting modules for use in polydimethylsiloxane-based microfluidic platforms have emerged; with the advent of paper-based microfluidic platforms, several specific volumetric metering principles were also developed. However, the current commercial pipettes are expensive and have narrow application range, and are not generally suitable for instant rapid diagnosis equipment. Furthermore, due to the urgent need for immediate and rapid diagnostics in developing countries, it becomes necessary to solve the low cost volumetric metering/pipetting problem.
The liquid transfer device is used for solving the problems that the current commercial liquid transfer device is expensive, has narrow application range and is not generally suitable for the instant rapid diagnosis equipment.
Disclosure of Invention
The invention aims to provide a dropping bottle manufacturing method for micro-liquid metering and liquid transferring, which can be used for repeatedly distributing samples or reagents with fixed volumes, and has low cost and wide application range.
In order to solve the technical problem, the invention provides a dropping bottle manufacturing method for metering and pipetting trace liquid, which comprises the following steps:
cutting a disposable pipette tip to obtain a tip of the pipette tip, wherein the inner diameter of the tip of the pipette tip is larger than that of a tip of a dropping bottle;
step two, sleeving the needle point of the gun head on the tip of the dropping bottle, and sealing and fixing the needle point of the gun head and the tip of the dropping bottle to obtain a combined tip of the dropping bottle;
taking a metal wire with a preset outer diameter, and penetrating the metal wire into the aperture of the combined tip of the dropping bottle;
step four, vertically placing the dropping bottle combined tip penetrated with the metal wire, heating the dropping bottle combined tip penetrated with the metal wire to enable the dropping bottle combined tip to be melted and attached to the metal wire, and then stopping heating;
step five, drawing the metal wire out of the heated dropping bottle combined tip;
and step six, cutting and removing the lower end part of the dropping bottle combined tip to obtain the dropping bottle combined tip with the target inner diameter.
Preferably, the first step includes:
the outer diameter of the dropping bottle tip is measured, the disposable pipette head is cut according to the measured outer diameter of the dropping bottle tip, and the cutting position of the disposable pipette head ensures that the corresponding outer diameter is more than or equal to 90% of the outer diameter of the dropping bottle tip.
Preferably, in the second step, "the tip of the gun head and the tip of the dropper bottle are sealed and fixed" specifically includes:
the tip of the gun head is hermetically connected with the tip of the drip bottle in a hot melting or gluing mode.
Preferably, the metal wire is a copper wire.
Preferably, the outer diameter of the wire is 0.05mm to 0.3 mm.
Preferably, in the fourth step, the metal wire-threaded dropper bottle combination tip is heated, and the heating source is positioned right below the dropper bottle combination tip.
Preferably, the heating source is an alcohol lamp.
Preferably, in the fourth step, the metal wire-threaded dropper combined tip is heated, and the heating source is arranged around the dropper combined tip.
Preferably, the step six specifically includes: and cutting and removing the lower end part of the dropper combination tip under a microscope to obtain the dropper combination tip with the target inner diameter.
Preferably, the disposable pipette tip is a 10 μ L disposable pipette tip
The invention has the beneficial effects that:
1. the invention has the advantages of simple and easily obtained raw materials, low cost, quick and simple manufacturing process, easy operation, wide application range and capability of designing the liquid transfer amount according to disposable liquid transfer gun heads with different specifications.
2. The invention can be used for repeatedly distributing samples or reagents with fixed volumes, can effectively improve the working efficiency of a plurality of testing processes needing repeated liquid transfer, and can also be used as a liquid transfer pipe for sucking and distributing samples or reagents with fixed volumes. Therefore, the invention can be used as a low-cost instant quick diagnosis dropper/pipettor.
Drawings
FIG. 1 is a schematic flow chart of the present invention for obtaining a dropper bottle assembly tip;
FIG. 2 is a schematic view of the heating of a combination spike of a dropper bottle of the present invention; wherein, (a) is a schematic diagram of the heating process of the combined tip of the dropping bottle; (b) the heating of the combined tip of the dropping bottle is shown.
The reference numbers in the figures illustrate: 10. a disposable pipette tip; 11. cutting to obtain a tip of the gun head; 12. a dropping bottle is to be modified; 13. a dropping bottle combined tip; 20. a metal wire; 30. a heat source.
Detailed Description
The present invention is further described below in conjunction with the following figures and specific examples so that those skilled in the art may better understand the present invention and practice it, but the examples are not intended to limit the present invention.
Referring to fig. 1, the invention discloses a method for manufacturing a dropping bottle for metering and pipetting a trace amount of liquid, which comprises the following steps:
step one, cutting the disposable pipette tip 10 to obtain a tip of the pipette tip, wherein the inner diameter of the tip of the pipette tip is larger than that of a tip of a dropper.
In this step, the outer diameter of the dropper tip can be measured, the disposable pipette tip 10 is cut according to the measured outer diameter of the dropper tip, and the cutting position of the disposable pipette tip 10 ensures that the corresponding inner diameter is greater than or equal to 90% of the outer diameter of the dropper tip. The inner diameter of the disposable gun head can be smaller than the outer diameter of the tip of the drip bottle, and because the materials are plastic, the disposable gun head can provide initial pretightening force through the external force sleeve during assembly, and interference fit is realized.
And step two, sleeving the tip of the gun head on the tip of the dropping bottle to realize clearance fit between the tip of the gun head and the tip of the dropping bottle, and sealing and fixing the tip of the gun head and the tip of the dropping bottle to obtain a combined tip 13 of the dropping bottle.
In this step, the tip of the gun head and the tip of the dropper bottle can be connected in a sealing way by hot melting or gluing.
And step three, taking the metal wire 20 with the preset outer diameter, and penetrating the metal wire 20 into the hole diameter of the dropper combination sharp head 13.
The metal wire 20 is a copper wire, and may be a metal wire made of other materials such as a steel wire. The outer diameter of the wire 20 is 0.05mm to 0.3mm, for example, the outer diameter of the wire may be 0.127mm or 0.2032 mm. The outer diameter of the wire 20 can be selected according to the operational requirements.
And step four, vertically placing the dropping bottle combined tip 13 penetrated with the metal wire 20, heating the dropping bottle combined tip 13 penetrated with the metal wire 20 to enable the dropping bottle combined tip 13 to be melted and attached to the metal wire 20, and then stopping heating.
During the heating process, the dropper combination tip 13 is elongated due to gravity, and the outer diameter of the dropper combination tip 13 becomes smaller.
In this step, the heating source 30 is located directly below the dropper combination tip 13, and the heating source 30 may be an alcohol burner. The heating source 30 may also be provided around the dropper tip 13, such as a heating wire.
And step five, drawing the metal wire 20 out of the heated dropping bottle combined sharp head 13.
The wire 20 is required to be slightly pulled out, and after the wire 20 is pulled out, the inside of the combination tip 13 of the dropping bottle is provided with a through hole with a specific hole diameter, and the hole diameter of the through hole is the same as the outer diameter of the wire 20.
And step six, cutting and removing the lower end part of the dropper combination sharp head 13 to obtain the dropper combination sharp head 13 with the target inner diameter. To enhance accuracy, the cut can be made under a microscope.
A disposable pipette tip 10 having a disposable pipette tip 10 of 10 may be selected for use in the present invention.
In the present invention, when using a dropper bottle or a pipette to transfer liquid, when the liquid drop is squeezed out, the gravity and the surface tension of the liquid drop will keep balance, and further squeezing will break the balance of the forces to cause the liquid drop to drop from the tip of the dropper bottle or the pipette, therefore, the inner diameter of the dropper bottle combination tip 13 determines the volume of the liquid drop per drop.
The invention can be used for repeatedly distributing samples or reagents with fixed volumes, can effectively improve the working efficiency of a plurality of testing processes needing repeated liquid transfer, and can also be used as a liquid transfer pipe for sucking and distributing samples or reagents with fixed volumes. Therefore, the invention can be used as a low-cost immediate and rapid diagnosis dropper/pipettor, namely POCT.
The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. The equivalent substitution or change made by the technical personnel in the technical field on the basis of the invention is all within the protection scope of the invention. The protection scope of the invention is subject to the claims.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010103403.4A CN111175086A (en) | 2020-02-19 | 2020-02-19 | Method for manufacturing dropping bottle for metering and transferring trace liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010103403.4A CN111175086A (en) | 2020-02-19 | 2020-02-19 | Method for manufacturing dropping bottle for metering and transferring trace liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111175086A true CN111175086A (en) | 2020-05-19 |
Family
ID=70625221
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010103403.4A Pending CN111175086A (en) | 2020-02-19 | 2020-02-19 | Method for manufacturing dropping bottle for metering and transferring trace liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111175086A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4309912A (en) * | 1980-08-01 | 1982-01-12 | Smith Kendall O | Micro-analysis process and device |
| US6066297A (en) * | 1997-01-03 | 2000-05-23 | Matrix Technologies Corporation | Small sample volume displacement pipette tips |
| US20080089811A1 (en) * | 2004-10-16 | 2008-04-17 | Olympus Life And Material Science Europa Gmbh | Pipetting Device |
| CN101553406A (en) * | 2006-12-07 | 2009-10-07 | 太阳医药高级研发有限公司 | Metered drop bottle for dispensing microliter amounts of a liquid in the form of a drop |
| US20160067705A1 (en) * | 2013-04-11 | 2016-03-10 | Brand Gmbh + Co Kg | Pipette device having a micro-dosing unit |
| CN206248379U (en) * | 2016-12-07 | 2017-06-13 | 重庆中元生物技术有限公司 | Portable micro sampling dilution Dropping feeder |
| US20190360898A1 (en) * | 2016-12-13 | 2019-11-28 | Hitachi, Ltd. | Dispensing device and dispensing method |
-
2020
- 2020-02-19 CN CN202010103403.4A patent/CN111175086A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4309912A (en) * | 1980-08-01 | 1982-01-12 | Smith Kendall O | Micro-analysis process and device |
| US6066297A (en) * | 1997-01-03 | 2000-05-23 | Matrix Technologies Corporation | Small sample volume displacement pipette tips |
| US20080089811A1 (en) * | 2004-10-16 | 2008-04-17 | Olympus Life And Material Science Europa Gmbh | Pipetting Device |
| CN101553406A (en) * | 2006-12-07 | 2009-10-07 | 太阳医药高级研发有限公司 | Metered drop bottle for dispensing microliter amounts of a liquid in the form of a drop |
| US20160067705A1 (en) * | 2013-04-11 | 2016-03-10 | Brand Gmbh + Co Kg | Pipette device having a micro-dosing unit |
| CN206248379U (en) * | 2016-12-07 | 2017-06-13 | 重庆中元生物技术有限公司 | Portable micro sampling dilution Dropping feeder |
| US20190360898A1 (en) * | 2016-12-13 | 2019-11-28 | Hitachi, Ltd. | Dispensing device and dispensing method |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200519 |