CN111135879B - A centrifugal microfluidic chip and sample processing method - Google Patents
A centrifugal microfluidic chip and sample processing method Download PDFInfo
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- CN111135879B CN111135879B CN201811313920.3A CN201811313920A CN111135879B CN 111135879 B CN111135879 B CN 111135879B CN 201811313920 A CN201811313920 A CN 201811313920A CN 111135879 B CN111135879 B CN 111135879B
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- 238000003672 processing method Methods 0.000 title claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 238000001514 detection method Methods 0.000 claims abstract description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 12
- 239000002699 waste material Substances 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 239000013039 cover film Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 239000010408 film Substances 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 238000007731 hot pressing Methods 0.000 claims 1
- 238000010586 diagram Methods 0.000 description 3
- 238000011166 aliquoting Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00495—Centrifuges
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1032—Dilution or aliquotting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention relates to a centrifugal microfluidic chip and a sample processing method. The centrifugal microfluidic chip comprises a liquid inlet hole, an equal-part hole, a first siphon pipeline, a sample pretreatment area, a second siphon pipeline, a reaction hole and a waste liquid pool which are sequentially connected, wherein the sample pretreatment area is also connected with an exhaust hole. The equal-volume hole comprises two chambers with equal volumes and is used for realizing equal-volume equal division of the sample; the volume of each reaction hole is equal to realize secondary equal division of the sample, and the sample pretreatment area and each reaction hole are loaded with different reaction reagents according to the requirement to realize multiple detection applications or the same reaction reagent to realize parallel detection of the same sample. The invention can realize pretreatment and multiple equal parts treatment of the sample, is convenient to operate and saves time.
Description
Technical Field
The invention belongs to the technical field of medical consumables, and particularly relates to a centrifugal microfluidic chip and a sample processing method adopting the centrifugal microfluidic chip, which can realize pretreatment and multiple equal-part treatment of samples.
Background
The centrifugal microfluidic chip belongs to medical consumables, is applied to the field of instant diagnosis, and can conveniently and rapidly output reaction results. The method is characterized in that a microfluidic structure is integrated on a wafer-shaped chip, and the flow of the microfluid is driven by centrifugal force, so that the detection and analysis of a sample are realized. The centrifugal system can complete operations such as sample pretreatment, mixing, accurate volume separation, detection and the like. In recent years, centrifugal microfluidic systems have been rapidly developed with the advantages of high throughput, high integration, multiple parallel analysis, portability, low cost, automation, small consumption of samples and reagents, and the like, and have been widely used in fields of biochemical detection, immunoassay, nucleic acid amplification, environmental monitoring, cell sorting, food safety, and the like.
Patent CN205379906U discloses a multipurpose microfluidic chip. The sample is distributed more uniformly by limiting the proportion of the cross section of the main channel, but the design of the cross section of the channel with narrow peaks and wide troughs of the main channel of the chip influences the flow velocity of liquid in the main channel, so that the problem of uniform sample distribution and the problem of bubble generation in the main channel cannot be completely solved.
Patent 108246373A discloses a centrifugal microfluidic chip, based on the U-shaped tube liquid seal principle, through the equal design of crest trough channel cross section of main channel, make the interior liquid velocity of flow of main channel even, realize the more even distribution of sample. The chip converts an unmeasured sample into a plurality of equal parts of accurate samples through capillary action and centrifugal force when the detection chip rotates, so that multi-index detection can be realized, but the structure is not suitable when the samples need pretreatment.
Disclosure of Invention
The invention aims at the problems and provides a centrifugal microfluidic chip and a sample processing method adopting the centrifugal microfluidic chip, which can realize pretreatment and multiple equal-part treatment of samples, are convenient to operate and save time.
The technical scheme adopted by the invention is as follows:
The centrifugal microfluidic chip comprises a liquid inlet hole, an equal part hole, a first siphon pipeline, a sample pretreatment area, a second siphon pipeline, a reaction hole and a waste liquid pool which are sequentially connected, wherein the sample pretreatment area is also connected with an exhaust hole.
Further, the equal-volume cavity comprises two equal-volume cavities for realizing equal-volume division of the sample.
Further, the number of the reaction holes is at least two.
Further, the volume of each reaction hole is equal to realize secondary aliquoting of the sample.
Further, the sample pretreatment area and each reaction well are loaded with different reagents as needed to realize multiple detection applications, or are loaded with the same reagents to realize parallel detection of the same sample.
Further, the reagent is loaded by one of freeze drying, vacuum drying and high temperature drying.
The sealing method is characterized by further comprising a cover plate or a cover film, wherein the cover plate or the cover film is used for forming a complete structure through sealing, and the sealing mode is one of ultrasonic bonding, thermocompression bonding and simple film pasting treatment.
A centrifugal microfluidic system comprises the centrifugal microfluidic chip and a centrifuge, wherein the centrifugal microfluidic chip is arranged on the centrifuge, and the centrifuge drives microfluid in the centrifugal microfluidic chip to flow through centrifugal force.
A sample processing method adopting the centrifugal microfluidic chip comprises the following steps:
1) Adding a certain amount of sample into the liquid inlet hole;
2) Starting the centrifugal machine, and after a certain time at a first rotation speed, realizing equal-quantity equipartition of samples at the equal-quantity holes;
3) Standing for a certain time to enable the sample to fill the first siphon pipeline through capillary action;
4) Starting the centrifugal machine for the second time, and enabling the sample to enter a sample pretreatment area after a certain time at a second rotating speed;
5) Standing for a certain time to enable the sample to fill the second siphon pipeline through capillary action;
6) And starting the centrifugal machine for the third time, and enabling the sample to sequentially enter the reaction hole and the waste liquid pool after a certain time at a third rotating speed.
Further, the first rotating speed in the step 2) is 100-3000rpm/s, the certain time is 20-40s, the second rotating speed in the step 4) is 100-3000rpm/s, the certain time is 30-100s, the third rotating speed in the step 6) is 100-3000rpm/s, and the certain time is 30-100s.
Compared with the prior art, the invention has the following beneficial effects:
1) The centrifugal microfluidic chip designed by the invention not only can realize the equal parts of samples, but also can perform pretreatment and secondary equal parts on the equal parts of samples, and is suitable for the situations that the samples need pretreatment and multiple equal parts.
2) The centrifugal microfluidic chip designed by the invention only needs one-step sample adding operation for operators, avoids a complicated sample pretreatment process, does not need professional training, is convenient to operate, and saves time.
Drawings
Fig. 1 is a schematic structural diagram of a centrifugal microfluidic chip in example 1.
Fig. 2 is a schematic structural diagram of a centrifugal microfluidic chip in example 2.
Detailed Description
The present invention will be further described in detail with reference to the following examples and drawings, so that the above objects, features and advantages of the present invention can be more clearly understood.
Example 1:
Fig. 1 is a schematic structural diagram of a centrifugal microfluidic chip 100 according to the present embodiment. The centrifugal microfluidic chip 100 comprises a liquid inlet 101, an equal-part hole 102, a first siphon pipeline 103, a sample pretreatment area 104, an exhaust hole 105, a second siphon pipeline 106, reaction holes 107 and 108 and a waste liquid pool 109. In FIG. 1, there are two sets 101-109, the two sets 101-109 being connected by 102.
The centrifugal microfluidic chip 100 is mounted on a centrifuge to form a centrifugal microfluidic system, so as to realize sample processing, and the method comprises the following steps:
1) A certain amount of sample is added to the inlet 101.
2) The centrifuge is started, the rotating speed is in the range of 100-3000rpm/s, and the time is in the range of 20-40 s. Since the equal-dividing hole 102 comprises two equal-dividing structures, namely two chambers with equal volumes, the liquid level after centrifugation is in the same radian, and equal-dividing of the sample can be realized at the equal-dividing hole 102.
3) After 10-60 seconds of rest the sample fills the first siphon 103 by capillary action.
4) The centrifuge is started a second time, at a speed in the range of 100-3000rpm/s, for a time in the range of 30-100s, and enters the sample pretreatment zone 104.
5) After 100-300 seconds of standing, the solution fills the second siphon 106 by capillary action.
6) The centrifuge is started for the third time, the rotating speed is in the range of 100-3000rpm/s, the time is in the range of 30-100s, and the solution sequentially enters the reaction holes 107 and 108 and the waste liquid pool 109. The volumes of the reaction wells 107, 108 may be set equal to achieve a secondary aliquoting of the solution at the reaction wells 107, 108.
Wherein 100 is used as a chip substrate and can be sealed with a cover plate or a cover film to form a complete structure. The sealing mode can be ultrasonic bonding, hot-press bonding or simple film pasting treatment.
The sample pretreatment area 104 and the reaction holes 107 and 108 can be loaded with different reagents according to the needs so as to realize various detection applications, and can also be loaded with the same reagents so as to realize parallel detection of the same sample. The reagent loading mode can be freeze drying, vacuum drying, high temperature drying and the like.
Wherein, after the third start of the centrifuge, the reaction holes 107, 108 are completely filled, and the surplus liquid enters the waste liquid pool 109.
Example 2:
in the structure of embodiment 1 shown in fig. 1, two groups 101 to 109 are provided on the centrifugal microfluidic chip 100. In this embodiment, more groups 101 to 109 are provided and uniformly distributed on the centrifugal microfluidic chip 100, such as the structure shown in fig. 2, so as to improve the processing efficiency of the sample.
Other structures of the centrifugal microfluidic chip of this embodiment are the same as those of embodiment 1.
Example 3:
in the structure of example 1 shown in FIG. 1, there are two reaction wells, i.e., 107, 108. In this embodiment, one or more than two reaction holes are provided.
Other structures of the centrifugal microfluidic chip of this embodiment are the same as those of embodiment 1.
The above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and those skilled in the art may modify or substitute the technical solution of the present invention without departing from the spirit and scope of the present invention, and the protection scope of the present invention shall be defined by the claims.
Claims (7)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811313920.3A CN111135879B (en) | 2018-11-06 | 2018-11-06 | A centrifugal microfluidic chip and sample processing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811313920.3A CN111135879B (en) | 2018-11-06 | 2018-11-06 | A centrifugal microfluidic chip and sample processing method |
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| Publication Number | Publication Date |
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| CN111135879A CN111135879A (en) | 2020-05-12 |
| CN111135879B true CN111135879B (en) | 2025-02-11 |
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| CN201811313920.3A Active CN111135879B (en) | 2018-11-06 | 2018-11-06 | A centrifugal microfluidic chip and sample processing method |
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| CN114660306A (en) * | 2020-12-22 | 2022-06-24 | 苏州含光微纳科技有限公司 | Centrifugal microfluidic chip liquid separation structure and liquid separation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103537329A (en) * | 2012-07-11 | 2014-01-29 | 三星电子株式会社 | Microfluidic structure, microfluidic device having the same and method of controlling the microfluidic device |
| CN205570360U (en) * | 2016-04-26 | 2016-09-14 | 杭州霆科生物科技有限公司 | Many indexs of multi -sample food additive detects micro -fluidic chip |
| CN209393198U (en) * | 2018-11-06 | 2019-09-17 | 苏州含光微纳科技有限公司 | A kind of centrifugal type microfludic chip and centrifugal type microfludic system |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101335727B1 (en) * | 2007-08-22 | 2013-12-04 | 삼성전자주식회사 | Centrifugal force-based disk type microfluidic device for blood chemistry analysis |
| WO2017027384A1 (en) * | 2015-08-07 | 2017-02-16 | Poc Medical Systems, Inc. | Microfluidic devices and methods of use thereof |
| CN206334683U (en) * | 2016-07-28 | 2017-07-18 | 上海速创诊断产品有限公司 | A kind of CD plate-likes micro-fluidic chip |
| CN207586245U (en) * | 2017-09-29 | 2018-07-06 | 深圳国际旅行卫生保健中心 | Centrifugal type microfludic chip |
| CN108490197B (en) * | 2018-03-07 | 2020-11-06 | 清华大学 | Multi-index analysis chip with pre-reaction function and method of using the same |
| CN108642141B (en) * | 2018-06-07 | 2021-09-10 | 国家纳米科学中心 | Nucleic acid detection reagent mixing and adding device |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103537329A (en) * | 2012-07-11 | 2014-01-29 | 三星电子株式会社 | Microfluidic structure, microfluidic device having the same and method of controlling the microfluidic device |
| CN205570360U (en) * | 2016-04-26 | 2016-09-14 | 杭州霆科生物科技有限公司 | Many indexs of multi -sample food additive detects micro -fluidic chip |
| CN209393198U (en) * | 2018-11-06 | 2019-09-17 | 苏州含光微纳科技有限公司 | A kind of centrifugal type microfludic chip and centrifugal type microfludic system |
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