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CN1111224A - PGI bacteria suspension for activating rhizosphere soil phosphorus and its preparation - Google Patents

PGI bacteria suspension for activating rhizosphere soil phosphorus and its preparation Download PDF

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CN1111224A
CN1111224A CN 94105175 CN94105175A CN1111224A CN 1111224 A CN1111224 A CN 1111224A CN 94105175 CN94105175 CN 94105175 CN 94105175 A CN94105175 A CN 94105175A CN 1111224 A CN1111224 A CN 1111224A
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culture
preparation
microbial inoculum
gram
phosphorus
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CN1061964C (en
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关桂兰
王卫平
杨玉锁
覃楠
陈理
孟颂东
郭朝辉
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XINJIANG INST OF BIOLOGY SOIL AND DESERT CHINESE ACADEMY OF SCIENCES
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XINJIANG INST OF BIOLOGY SOIL AND DESERT CHINESE ACADEMY OF SCIENCES
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Abstract

The said PG1 bacteria are separated from the soil around the roots of some medicine plant and it is one species of pseudomonas with strong reproductive capacity. PG1 bacteria produce gibberellin and cytokinin promoting the growth of root system and have strong phosphate-decomposing effect promoting the coversion of insoluble phosphate. Its use can increase the yield of wheat.

Description

PGI bacteria suspension for activating rhizosphere soil phosphorus and its preparation
The present invention relates to a kind of PG that is used for the activating rhizosphere soil phosphorus and farm crop is had remarkable production-increasing function 1Microbiobacterial agent and preparation method thereof.
Worldwide, it is very wide that soil lacks the phosphorus area distributions, and especially in arid, semiarid zone, neutral and meta-alkalescence soil available phosphorus more lacks.In order to improve crop yield, apply various phosphate fertilizer, though available phosphorus has increase in the soil, but most of phosphorus still is fixed in the soil.The utilization ratio of phosphate fertilizer is greatly between 5-25%.Phosphate fertilizer price increase both at home and abroad in recent years, the phosphorus ore grade descends, and the phosphate fertilizer source is subjected to restriction to a certain degree.The utilization ratio of the utilization of phosphorus and raising phosphate fertilizer is still the major issue in the agriculture production in the soil.For this reason, countries in the world are for utilizing soil microorganisms to improve the rhizosphere condition, and the activating rhizosphere soil phosphorus strengthens the conversion of indissoluble phosphorus, improves phosphate fertilizer utilization efficiency, gives to pay close attention to widely.China's phosphorus bacteria research starting is early screened and is imposed in the soil as phosphorus bacteria fertilizer but be limited to phosphate-solubilizing bacteria more, and effect is very not remarkable.
The object of the invention is, the activating rhizosphere soil phosphorus of development also has the PG of production-increasing function 1Microbial inoculum and preparation method thereof is according to root system, soil and rhizospheric microorganism interaction principle, and the directed change rhizosphere microflora is preponderated its useful rhizospheric microorganism, improves rhizosphere environment, promotes crop growthing development, improves crop yield.
Task of the present invention is: PG 1Bacterial strain is a kind in the isolated Pseudomonas (Pseudomonas) from plant rhizosphere soil, reproductivity is strong, and fermentation culture can reach 20,000,000,000/milliliter, low temperature resistant and high temperature in 24-36 hour, salt tolerant alkali and arid have stronger resistivity to adverse circumstance.PG 1Microbial inoculum seed dressing is bonded on the seed, enters after the soil with seed, and with the seed germination growth, after taking root, a large amount of breedings, after the local temperature rise, when the crop root ramp is grown, PG 1Bacterium breeds and is gathered in rhizosphere in a large number, preponderate in rhizosphere microflora (confirming with the antibiotic labelling method).PG 1Produce organic acid, reduce root district pH value, make the rhizosphere environment acidifying, promote the conversion of indissoluble phosphorus, improve the rhizosphere soil available phosphorus content.While PG 1Bacterium produces Plant hormones regulators,gibberellins and bacterium mitogen, can promote root growth and development, promotes the transportation and the absorption of phosphorus.
The activating rhizosphere soil phosphorus of the present invention development also has the PG of production-increasing function 1Microbial inoculum and preparation method thereof belongs to a kind PG in the Rhodopseudomonas (Pseudomonas) through evaluation 1And at Beijing China Committee for Culture Collection of Microorganisms common micro-organisms center (CGNCC) preservation, No.0208.
The activating rhizosphere soil phosphorus of the present invention development also has the PG of production-increasing function 1Microbial inoculum and preparation method thereof, its principal feature:
(a), PG 1The bacterial strain colonial morphology, garden shape, moistening on the BPM flat board, neat in edge, projection, clear, colorless element;
PG 1The test tube slant cultural characteristic, projection is produced more mucus, and is khaki, the liquid culture muddiness;
PG 1Individual morphology: the straight thin bar attitude of cell, 0.5-0.6 * 2-3 μ n, Gram-negative, no gemma, polar flagella, motion, protoplasm dyeing is inhomogeneous.
(b), PG 1The bacterium physicochemical property: catalase reaction is positive; The catalase reaction is positive; The arginine dihydrolase reaction is positive.Aerobic, nonfermented, not aerogenesis.The V.P reaction negative; PH value dropped to 5.4 after the V.P nutrient solution was grown 4 days, and the methyl red experiment is positive; Hydrolyzed casein; Liquefy gelatin, hydrolyzed starch does not reduce nitrate; Sucrose hydrolysis does not form Polylevulosan; Produce soluble pigment, do not produce carotenoids, aspect utilization of carbon source, can utilize glycerine, gluconate, malonate, N.F,USP MANNITOL, pectinose, glucose, wood sugar, inositol, rhamnosyl, trehalose, sorbyl alcohol, ribose and Citrate trianion.Do not utilize tartrate, lactose and sucrose.On the substratum of saltiness 7% on the PH7.2-7.5 substratum, can grow.95 ℃ of the highest tolerable temperatures are minimum at 0-4 ℃; All can grow for 20-40 ℃.
It mainly is that (a), former spawn culture based formulas are (is radix with 1000 milliliters of capacity) extractum carnis 0.8-4 gram on the preparation method, peptone 1-4 gram, with pol is fixed molten 1000 milliliters of the wort of 7 °-10 ° in Bahrain, transfers PH7.0-7.5 with sodium hydroxide, agar powder 18-20 gram, culture temperature 20-30 ℃, activate twice, be 36-38 hour at every turn, enters triangular flask or eggplant bottle shaking table and cultivate, time is 24-36 hour, connects the seeding tank fermentation culture then;
(b), liquid fermentation medium prescription: Semen Maydis powder 2-4%, soybean cake powder 1-2%, calcium chloride 0.1-0.3%, sulfate of ammoniac 0.4-0.6%, SODIUMNITRATE 0.1-0.3%, Sodium phosphate dibasic 0.2-0.4%, zinc chloride 0.1-0.2%, pol are that wort 2-4%, the surplus of 7 °-10 ° in Bahrain is water, transfer PH7.2-7.5, changing big jar of culture temperature over to is 25-30 ℃, air flow is 1: 0.5-0.8, the time is 24-36 hour.Other operation is all carried out the routine sterilization by microbial liquid fermentation requirement, available light calcium carbonate absorption in aftertreatment, oven dry then, crushing packing.
The activating rhizosphere soil phosphorus of the present invention development also has the PG of production-increasing function 1Microbial inoculum and preparation method thereof is through a large amount of evidences, PG 1Microbial inoculum effect on phosphorus decomposing and increasing crop yield is extremely remarkable.PG particularly 1On the inorganic phosphorus culture medium flat plate, show stronger phosphate solubilization, inoculate back 2 days phosphorus decomposing loop diameters and reach 2-4 centimetre, cultivate, cultivated 3 days for 28 ℃, contrast and be 0.25ppm, inoculation PG with inorganic phosphorus decomposing medium liquid 1Microbial inoculum reaches 50.0ppm, and for contrasting 200 times, cultivating 7 days is 307 times of contrasts, cultivates to reach 640 times in 15 days.Do activation rhizosphere soil phosphorus transformation experiment with culturing pot and show, inoculation rhizosphere soil available phosphorus contents average out to 49ppm contrasts and is 12ppm, is 4 times of contrast.Show that with the further checking of peviform culturing pot survey the rapid available phosphorus of its rhizosphere soil, contrast on average is 20.0ppm, handling on average is 48.6ppm, is 2.3 times of contrast.From the above PG that experimental results show that 1Microbial inoculum has stronger conversion power to indissoluble phosphorus in the rhizosphere soil, can improve the content of the available phosphorus of rhizosphere soil, by plant is utilized.In addition, PG 1Bacterium also secretes Plant hormones regulators,gibberellins and phytokinin, inoculation PG 1Bacteria fermentation reaches 0.29ppm and 0.35ppm respectively after 2 days, Plant hormones regulators,gibberellins and phytokinin promote the transportation of phosphorus in the plant materials and the absorption of phosphorus, and promotes plant-growth and tiller the number of productive ear of raising wheat crop.PG 1Microbial inoculum application wheat, barley, seeding corn and other crops have production-increasing function by a relatively large margin.PG 1The seed dressing wheat is potted plant, and output improves 59.7%, improves 117.7% after adding phosphate fertilizer.Field plot trial improves output 63.7%.Land for growing field crops wheat and barley test increase rate are also bigger, are respectively 36.5% and 30.6%.Corn field plot trial volume increase 27.6%, land for growing field crops volume increase 21.5%, beet increases sugariness 1.3-2.5%.Crops such as air exercise melon seeds, rape, cotton, beans all have production-increasing function in various degree.
The activating rhizosphere soil phosphorus that the present invention developed also has the PG of production-increasing function 1Microbial inoculum and preparation method thereof, its using method is:
Seed dressing: dress seed before the crop seeding, the big kinds of crop seeding amount such as wheat are with 60-80 milliliter PG 1Microbial inoculum is mixed the grain weight of 0.07 hectare.Medium level is the 40-50 milliliter as seed dressing amounts such as corns during sowing, mixes the 0.07 hectare grain weight, for the less picture rape etc. of application rate, seed dressing amount 20-30 milliliter.Seed dressing must be even, makes every seed all can be stained with microbial inoculum.
Be stained with root: the farm crop of transplanting can make microbial inoculum enter the root district with the method for being stained with root as paddy rice and vegetables, tomato, spicy etc.
Spray: spray with every mu of ground 100-150 milliliter microbial inoculum dilution in vegetative period as cotton, melon etc. for cash crop.
Embodiment 1:
(1) at first prepares PG 1Microbial inoculum
Former spawn culture: (is radix with 1000 milliliters of capacity) restrains with extractum carnis 1.5 grams, peptone 2, with pol is fixed molten 1000 milliliters of the wort of 7 °-10 ° in Bahrain, transfer PH7.0-7.5 with sodium hydroxide, again agar powder 18 is restrained in the wort mixed solution that is dissolved in 1000 milliliters, 25 ℃ of culture temperature activate twice, are 36-38 hour at every turn, enter triangular flask or eggplant bottle shaking table and cultivate, connect the seeding tank fermentation culture then; Time is 24-36 hour.Wherein liquid fermentation and culture is that to get Semen Maydis powder 2%, soybean cake powder 2%, calcium chloride 0.3%, ammonium sulfate 0.5%, SODIUMNITRATE 0.2%, Sodium phosphate dibasic 0.4%, zinc chloride 0.2%, pol be that wort 3%, the surplus of 7 °-10 ° in Bahrain is water, fully stir and be mixed, transfer PH7.2-7.5, changing big jar over to cultivates, time is 24-36 hour, air flow is 1: 0.5-0.8, temperature is 25 ℃, adopts light calcium carbonate absorption, oven dry, crushing packing to get final product (all carrying out the routine sterilization by microbial liquid fermentation requirement in operation) in aftertreatment.
(2) using method:
With 10 mu of winter wheat lands for growing field crops, contrast is radix for 3 mu, takes by weighing 25 kilograms of wheat seeds, uses PG 180 milliliters of seed dressings of microbial inoculum, its result is surveyed in sowing immediately after the seed dressing during results, and every mu of ground increases by 740,000 of number of productive ears, and thousand seed weight increases by 0.14 gram, and every mu of ground increases by 112 kilograms, and stimulation ratio is 36.5%.
Embodiment 2:
(1) at first prepares PG 1Microbial inoculum
Former spawn culture: (is radix with 1000 milliliters of capacity) restrains with extractum carnis 3 grams, peptone 4, with pol is fixed molten 1000 milliliters of the wort of 7 °-10 ° in Bahrain, transfer PH7.0-7.5 with sodium hydroxide, again agar powder 2 is restrained in the wort mixed solution that is dissolved in 1000 milliliters, 30 ℃ of culture temperature activate twice, and the time is each 36-38 hour, enter triangular flask or eggplant bottle shaking table and cultivate, connect the seeding tank fermentation culture then; Time is 24-36 hour.Wherein liquid fermentation and culture is that to get Semen Maydis powder 4%, soybean cake powder 1%, calcium chloride 0.15%, ammonium sulfate 0.25%, SODIUMNITRATE 0.3%, Sodium phosphate dibasic 0.25%, zinc chloride 0.15%, pol be that wort 4%, the surplus of 7 °-10 ° in Bahrain is water, fully stir and be mixed, transfer PH7.2-7.5, changing big jar over to cultivates, time is 36 hours, air flow is 1: 0.5-0.8, temperature is 30 ℃, adopts bottled in aftertreatment or adopts light calcium carbonate absorption, oven dry, crushing packing get final product (all carrying out routine by microbial liquid fermentation requirement in will operating sterilizes).
(2) using method:
With 6 mu of barley fields, 7 mu of barleys of contrast is radix, takes by weighing 15 kilograms of wheat seeds, uses PG 1Sowing is immediately mixed evenly in 50 milliliters of seed dressings of microbial inoculum, surveys its result during results, and every mu of ground increases by 340,000 of number of productive ears, and thousand seed weight increases by 0.26 gram, and every mu of ground increases by 92 kilograms, and stimulation ratio is 30.6%.
Embodiment 3:
(1) at first prepares PG 1Microbial inoculum
Former spawn culture: (is radix with 1000 milliliters of capacity) restrains with extractum carnis 4 grams, peptone 3, with pol is fixed molten 1000 milliliters of the wort of 7 °-10 ° in Bahrain, transfer PH7.0-7.5 with sodium hydroxide, again agar powder 15 is restrained in the wort mixed solution that is dissolved in 1000 milliliters, 30 ℃ of culture temperature activate twice, and the time is each 36-38 hour, enter triangular flask or eggplant bottle shaking table and cultivate, connect the seeding tank fermentation culture then; Time is 24-36 hour.Wherein liquid fermentation and culture is that to get Semen Maydis powder 3%, soybean cake powder 1.5%, calcium chloride 0.2%, ammonium sulfate 0.5%, SODIUMNITRATE 0.2%, Sodium phosphate dibasic 0.3%, zinc chloride 0.15%, pol be that wort 2.5%, the surplus of 7 °-10 ° in Bahrain is water, fully stir and be mixed, transfer PH7.2-7.5, changing big jar over to cultivates, time is 30 hours, air flow is 1: 0.5-0.8, temperature is 30 ℃, adopts light calcium carbonate absorption, oven dry, crushing packing to get final product (all carrying out the routine sterilization by microbial liquid fermentation requirement in operation) in aftertreatment.
(2) using method:
With 1 mu of ground is the application rate PG that radix takes by weighing 1 mu of corn 150 milliliters of seed dressings of microbial inoculum, its result is surveyed in sowing immediately after the seed dressing during results, every mu of ground volume increase 27.6%.
Figure 941051757_IMG1

Claims (3)

1, a kind of PG that is used for the activating rhizosphere soil phosphorus and production-increasing function is arranged 1Microbial inoculum and preparation method thereof is characterized in that it is a kind PG in the Pseudomonas (Pseudononas) 1All at China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation (CGNCC) No.0208.
2, activating rhizosphere the soil phosphorus according to claim 1 and PG of production-increasing function is arranged 1Microbial inoculum and preparation method thereof is characterized in that:
(a), PG 1The bacterial strain colonial morphology, garden shape, moistening on the BPN flat board, neat in edge, projection, clear, colorless element;
PG 1The test tube slant cultural characteristic, projection is produced more mucus, and is khaki, the liquid culture muddiness;
PG 1Individual morphology: the straight thin bar attitude of cell, 0.5-0.6 * 2-3 μ n, Gram-negative, no gemma, polar flagella, motion, protoplasm dyeing is inhomogeneous.
(b), PG 1The bacterium physicochemical property: catalase reaction is positive; The catalase reaction is positive; The arginine dihydrolase reaction is positive.Aerobic, nonfermented, not aerogenesis.The V.P reaction negative; PH value dropped to 5.4 after the V.P nutrient solution was grown 4 days, and the methyl red experiment is positive; Hydrolysis is extremely plain; Liquefy gelatin, hydrolyzed starch does not reduce nitrate; Sucrose hydrolysis does not form Polylevulosan; Produce soluble pigment, do not produce carotenoids, aspect utilization of carbon source, can utilize glycerine, gluconate, malonate, N.F,USP MANNITOL, pectinose, glucose, wood sugar, inositol, rhamnosyl, trehalose, sorbyl alcohol, ribose and Citrate trianion.Do not utilize tartrate, lactose and sucrose.On the substratum of saltiness 7% on the PH 7.2-7.5 substratum, can grow.95 ℃ of the highest tolerable temperatures are minimum at 0-4 ℃; All can grow for 20-40 ℃.
3, activating rhizosphere soil phosphorus according to claim 1 and have production-increasing function that PG is arranged 1Microbial inoculum and preparation method thereof is characterized in that:
(a), former spawn culture based formulas is (is radix with 1000 milliliters of capacity) extractum carnis 0.8-4 gram, peptone 1-4 gram, with pol is fixed molten 1000 milliliters of the wort of 7 °-10 ° in Bahrain, transfers PH7.0-7.5 with sodium hydroxide, agar powder 18-20 gram, culture temperature 20-30 ℃, activate twice, be 36-38 hour at every turn, enters triangular flask or eggplant bottle shaking table and cultivate, time is 24-36 hour, connects the seeding tank fermentation culture then;
(b), liquid fermentation medium prescription: Semen Maydis powder 2-4%, soybean cake powder 1-2%, calcium chloride 0.1-0.3%, sulfate of ammoniac 0.4-0.6%, SODIUMNITRATE 0.1-0.3%, Sodium phosphate dibasic 0.2-0.4%, zinc chloride 0.1-0.2%, pol are that wort 2-4%, the surplus of 7 °-10 ° in Bahrain is water, transfer PH 7.2-7.5, changing big jar of culture temperature over to is 25-30 ℃, air flow is 1: 0.5-0.8, the time is 24-36 hour.
CN 94105175 1994-05-06 1994-05-06 PGI bacteria suspension for activating rhizosphere soil phosphorus and its preparation Expired - Fee Related CN1061964C (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100400648C (en) * 2005-12-23 2008-07-09 南京农业大学 A high-efficiency phosphorus-accumulating bacterium and the bacterial agent produced therewith
CN102174540B (en) * 2000-06-16 2013-08-07 托马斯·施穆林 Methods to alter plant morphology, biochemistry and physiology
CN104045383A (en) * 2014-06-17 2014-09-17 贵阳飞龙雨实业有限公司 Preparation method of compound microorganism fertilizer for planting nursery stock
CN107089848A (en) * 2016-12-20 2017-08-25 广西壮族自治区林业科学研究院 A kind of forest land Phosphorus in Red Soil activates microbial inoculum
CN118165892A (en) * 2024-05-09 2024-06-11 南京农业大学三亚研究院 A Pseudomonas composite bacterial agent and its application

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102174540B (en) * 2000-06-16 2013-08-07 托马斯·施穆林 Methods to alter plant morphology, biochemistry and physiology
CN102174541B (en) * 2000-06-16 2014-03-19 托马斯·施穆林 Methods to alter plant morphology, biochemistry and physiology
CN100400648C (en) * 2005-12-23 2008-07-09 南京农业大学 A high-efficiency phosphorus-accumulating bacterium and the bacterial agent produced therewith
CN104045383A (en) * 2014-06-17 2014-09-17 贵阳飞龙雨实业有限公司 Preparation method of compound microorganism fertilizer for planting nursery stock
CN107089848A (en) * 2016-12-20 2017-08-25 广西壮族自治区林业科学研究院 A kind of forest land Phosphorus in Red Soil activates microbial inoculum
CN118165892A (en) * 2024-05-09 2024-06-11 南京农业大学三亚研究院 A Pseudomonas composite bacterial agent and its application
CN118165892B (en) * 2024-05-09 2024-07-05 南京农业大学三亚研究院 A Pseudomonas composite bacterial agent and its application

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