CN111097067A - A kind of antibacterial medical dressing for promoting rapid wound healing and preparation method thereof - Google Patents
A kind of antibacterial medical dressing for promoting rapid wound healing and preparation method thereof Download PDFInfo
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
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- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0019—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
- A61L2300/406—Antibiotics
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
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Abstract
本发明属于医用敷料领域，具体涉及一种促伤口快速愈合的抗菌性医用敷料及其制备方法。本发明的医用敷料通过将血管内皮生长因子、丝素蛋白、海藻酸盐和抗菌药物等多种活性成分进行联用，不仅能够实现长效抑菌，防止创面感染，同时在促进创面肉芽组织快速生长方面具有协同增效作用，进而实现伤口快速愈合的目的，治疗结束后无复发现象。The invention belongs to the field of medical dressings, and in particular relates to an antibacterial medical dressing for promoting rapid wound healing and a preparation method thereof. By combining various active ingredients such as vascular endothelial growth factor, silk fibroin, alginate and antibacterial drugs, the medical dressing of the present invention can not only achieve long-term bacteriostasis, prevent wound infection, but also promote the rapid development of wound granulation tissue. It has a synergistic effect in terms of growth, thereby achieving the purpose of rapid wound healing, and there is no recurrence after treatment.
Description
Technical Field
The invention belongs to the field of medical dressings, and particularly relates to an antibacterial medical dressing for promoting rapid wound healing and a preparation method thereof.
Background
A wound may be defined as a skin defect caused by mechanical, electrical, thermal, chemical, etc. factors or due to physiological metamorphosis. Clinically, poor wound healing includes necrosis of wound margins, skin necrosis, skin erosion, incision dehiscence, fat liquefaction, sinus formation, hematoma formation, etc., and the incidence of these complications is 2-37%. The non-healing of the wound brings great pain to the patient and also occupies a large amount of medical resources. For some wounds which are difficult to heal, the common bandaging treatment is often difficult to heal, and special means are needed. The wound is difficult to heal, and human immune cells are damaged due to certain systemic diseases; insufficient cell regeneration ability due to aging, physical weakness, malnutrition, etc.; and wound infection, inflammatory reaction, etc., which are caused by poor collagen synthesis and impaired fibroblast metabolism.
The use of dressings is an important means of maintaining a good healing environment for wounds. Traditional dressings are used for wounds to mainly play a role in isolation and bacteriostasis, but often cause the wounds to be dry, destroy healthy growth factors and easily adhere to new tissues, and secondary trauma to the wounds can be caused when the dressings are removed. With the improvement of living standard and medical standard of people, people also put higher requirements on the dressing. The medical dressing is used as a wound surface covering material, can replace damaged skin to play a role of temporary barrier in the wound healing process, keeps a balanced exchange environment of moisture and gas, avoids or controls wound infection, and provides a good healing environment for the wound surface. However, few medical dressings can achieve the effects of controlling infection, helping cell proliferation and promoting wound healing. Therefore, in order to better meet the requirements of clinical treatment and use, the development of a novel antibacterial medical dressing for promoting the rapid healing of wounds is urgently needed in the market, and has important clinical significance in the aspect of treating skin wounds.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an antibacterial medical dressing for promoting the rapid healing of wounds and a preparation method thereof. The medical dressing combines a plurality of active ingredients such as vascular endothelial growth factor, silk fibroin, alginate, antibacterial drug and the like, so that long-acting bacteriostasis can be realized, wound infection can be prevented, wound granulation tissues can be effectively promoted to grow rapidly and orderly, and the purpose of rapid healing of wounds can be further realized.
In order to solve the technical problems, the invention adopts the technical scheme that:
an antibacterial medical dressing for promoting rapid wound healing comprises the following raw material components in percentage by weight: 0.2-0.6% of vascular endothelial growth factor, 1-2% of silk fibroin aqueous solution, 2-4% of alginate, 1-6% of sodium hyaluronate, 1-3% of antibacterial drug, 20-30% of polyethylene glycol, 5-9% of carbomer and the balance of deionized water.
Preferably, the antibacterial medical dressing comprises the following raw material components in percentage by weight: 0.3-0.5% of vascular endothelial growth factor, 1.2-1.8% of silk fibroin aqueous solution, 2.5-3.5% of alginate, 2-4% of sodium hyaluronate, 1.5-2.5% of antibacterial drug, 22-28% of polyethylene glycol, 6-8% of carbomer and the balance of deionized water.
Preferably, the antibacterial medical dressing comprises the following raw material components in percentage by weight: 0.4% of vascular endothelial growth factor, 1.6% of silk fibroin aqueous solution, 3% of alginate, 3% of sodium hyaluronate, 2% of antibacterial drug, 26% of polyethylene glycol, 7% of carbomer and the balance of deionized water.
Preferably, the concentration of the silk fibroin aqueous solution is 10-20%.
Preferably, the alginate is selected from one or more of sodium alginate, zinc alginate, calcium alginate and magnesium alginate, and the molecular weight range is 10000-.
Preferably, the antibacterial drug is one or more of moxifloxacin hydrochloride, chlorhexidine, levofloxacin and gentamicin.
Preferably, the polyethylene glycol is selected from one or more of polyethylene glycol 400, polyethylene glycol 600, polyethylene glycol 800 and polyethylene glycol 1000.
Furthermore, the invention also provides application of the antibacterial medical dressing in preparing a surgical wound material for promoting rapid wound healing.
Further, the invention also provides a preparation method of the antibacterial medical dressing for promoting the wound to heal quickly, which comprises the following steps: putting the vascular endothelial growth factor, the silk fibroin aqueous solution, the alginate, the sodium hyaluronate, the polyethylene glycol, the carbomer and a proper amount of deionized water into a container according to the weight ratio, stirring and mixing to fully dissolve all the components, then adding the antibacterial drug and the rest of the deionized water, stirring uniformly, and performing sterilization treatment to obtain the antibacterial medical dressing.
Preferably, the sterilization treatment adopts Co-60 gamma ray irradiation for sterilization, and the sterilization dose is 60-80 kGy.
Vascular endothelial growth factor was first discovered in serum-free tumor cell culture and in animal cancerous ascites in 1979 by Dvorak et al, and was identified as a dimeric heparin affinity glycoprotein bound by disulfide bonds after analysis by Senger et al (1983) and was designated as Vascular Permeability Factor (VPF). Gospodalowicz and Ferrara et al (1989) isolated from bovine pituitary follicular cells an analog named as Vascular Endothelial Growth Factor (VEGF), which was analyzed to be the same cytokine with specificity for vascular endothelial cells. The vascular endothelial growth factor is an endogenous bioactive substance existing in a human body, can stimulate the proliferation of various cells, and has the effects of promoting the growth, proliferation and synthesis of the cells. VEGF can regulate the wound repair process and is an important factor influencing wound healing, so that the VEGF has good therapeutic effect on various diseases such as various wounds, burns, scalds and ulcers.
Silk Fibroin (SF) is a natural high molecular protein, contains 18 amino acids, and has good biocompatibility and biodegradability. The silk fibroin has the functions of promoting the regeneration of skin tissues and healing wounds, and the healing speed of skin wounds treated by smearing the silk fibroin is faster than that of skin wounds treated by normal saline. The wound dressing with good sealing property, antibacterial property and moisture retention plays an important role in wound healing. The characteristics of air permeability, strong moisture retention, easy shape plasticity, safety and no toxicity of the silk fibroin make the silk fibroin become a candidate material for preparing the skin wound dressing. The silk fibroin wound dressing developed at present mainly comprises silk fibroin films, silk fibroin gel, silk fibroin sponge, silk fibroin nano fibers, a silk fibroin wound dressing loaded with growth factors and plant active ingredients and the like, and can meet various wound treatments with different requirements.
Alginic acid is a natural polysaccharide widely found in brown algae, and dried brown algae generally contains about 20% alginic acid. Because the quantity of guluronic acid (G) and mannuronic acid (M) contained in alginic acid molecules is different, and the composition and relative molecular mass of alginic acid also show diversity, the alginate and the mixture thereof have functional diversity, namely good hygroscopicity, easy uncovering property, high oxygen permeability, gel blocking property, biodegradability, compatibility, metal ion adsorbability and the like, and are widely applied to the medical field, especially to the hemostasis of various wounds as wound dressings. Alginate can absorb 20 times of liquid by self mass due to the action of the hydrophilic group and the particularity of the three-dimensional structure, while the non-wet dressing commonly used in clinic at present only has 14 to 20 percent of the capacity. In addition, alginate forms gel on the surface of the wound in the process of swelling to saturation after absorbing water, and the structure not only ensures a moist environment required for healing, but also further ensures the tightness of the wound. Meanwhile, calcium alginate formed in the cross-linking process can adsorb bacteria and prevent the bacteria from entering the wound surface in the healing process, and can continuously release calcium ions to the wound to induce platelet activation, so that the healing of the wound surface is accelerated.
Compared with the prior art, the invention has the beneficial effects that: the inventor unexpectedly discovers that the medical dressing disclosed by the invention can realize long-acting bacteriostasis and prevent wound infection by combining a plurality of active ingredients such as vascular endothelial growth factor, silk fibroin, alginate and antibacterial drugs, and has a synergistic effect on promoting the rapid growth of granulation tissues of the wound so as to realize the purpose of rapid healing of the wound, and no recurrence phenomenon exists after the treatment is finished. The medical dressing has definite curative effect and safe and reliable use, can be used for wounds such as surgical wounds, burns, scalds and the like, plays the roles of analgesia, hemostasis, inflammation diminishing and antibiosis, does not cause stimulation to skin, has quick and simple preparation method and has wide practical application value.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
Example 1
An antibacterial medical dressing for promoting rapid wound healing comprises the following raw material components in percentage by weight: 0.2% of vascular endothelial growth factor, 2% of silk fibroin aqueous solution, 2.5% of sodium alginate, 2% of sodium hyaluronate, 3% of levofloxacin, 40020% of polyethylene glycol, 8% of carbomer and the balance of deionized water.
Among them, vascular endothelial growth factor was purchased from Sigma, usa; the silk fibroin is purchased from Sichuan bioscience GmbH (molecular weight: 5000-; alginate was purchased from jonan dongxuan bioengineering ltd; other products and reagents are all conventional and commercially available.
The preparation method of the antibacterial medical dressing for promoting the rapid healing of the wound comprises the following steps: putting the vascular endothelial growth factor, the silk fibroin aqueous solution, the sodium alginate, the sodium hyaluronate, the polyethylene glycol 400, the carbomer and a proper amount of deionized water into a container according to the weight ratio, stirring and mixing to fully dissolve all the components, then adding the levofloxacin and the rest deionized water, stirring uniformly, and performing sterilization treatment to obtain the antibacterial medical dressing.
Example 2
An antibacterial medical dressing for promoting rapid wound healing comprises the following raw material components in percentage by weight: 0.5% of vascular endothelial growth factor, 1.8% of silk fibroin aqueous solution, 2.8% of calcium alginate, 4% of sodium hyaluronate, 2.5% of gentamicin, 60025% of polyethylene glycol, 6% of carbomer and the balance of deionized water.
The preparation method of the antibacterial medical dressing for promoting the rapid healing of the wound is the same as that of the example 1.
Example 3
An antibacterial medical dressing for promoting rapid wound healing comprises the following raw material components in percentage by weight: 0.4% of vascular endothelial growth factor, 1.6% of silk fibroin aqueous solution, 3% of zinc alginate, 3% of sodium hyaluronate, 2% of moxifloxacin hydrochloride, 80026% of polyethylene glycol, 7% of carbomer and the balance of deionized water.
The preparation method of the antibacterial medical dressing for promoting the rapid healing of the wound is the same as that of the example 1.
Comparative example 1(in contrast to example 3, no vascular endothelial growth factor)
An antibacterial medical dressing for promoting rapid wound healing comprises the following raw material components in percentage by weight: 1.8% of silk fibroin aqueous solution, 3.2% of zinc alginate, 3% of sodium hyaluronate, 2% of moxifloxacin hydrochloride, 80026% of polyethylene glycol, 7% of carbomer and the balance of deionized water.
The preparation method of the antibacterial medical dressing for promoting the rapid healing of the wound is the same as that of the example 1.
Comparative example 2(in contrast to example 3, without aqueous fibroin solution)
An antibacterial medical dressing for promoting rapid wound healing comprises the following raw material components in percentage by weight: 1% of vascular endothelial growth factor, 4% of zinc alginate, 3% of sodium hyaluronate, 2% of moxifloxacin hydrochloride, 80026% of polyethylene glycol, 7% of carbomer and the balance of deionized water.
The preparation method of the antibacterial medical dressing for promoting the rapid healing of the wound is the same as that of the example 1.
Comparative example 3(in contrast to example 3, without zinc alginate)
An antibacterial medical dressing for promoting rapid wound healing comprises the following raw material components in percentage by weight: 1% of vascular endothelial growth factor, 4% of silk fibroin aqueous solution, 3% of sodium hyaluronate, 2% of moxifloxacin hydrochloride, 80026% of polyethylene glycol, 7% of carbomer and the balance of deionized water.
The preparation method of the antibacterial medical dressing for promoting the rapid healing of the wound is the same as that of the example 1.
Experiment of bacteriostatic effect
The antibacterial medical dressings of examples 1 to 3 were subjected to the experiment of the antibacterial effect with reference to the operation of 2012 edition "disinfection technical Specification" of Ministry of public health of the people's republic of China, and meanwhile, the medical dressings were used for medical purposes without adding antibacterial drugsThe dressing served as a blank. The experimental process comprises the following steps: prepared at a concentration of 105-106CFU/ml bacterial suspension. Adding 1ml of bacterial suspension into 10ml of liquid medical dressing, mixing uniformly, keeping contact for 20-30 minutes, diluting by 10 times, taking 0.5ml of liquid, coating the liquid on a sterile plate, pouring 20-25ml of sterile agar culture medium with the temperature of 40-50 ℃, culturing for 48-72 hours at 37 ℃, counting colonies, and calculating the bacteriostasis rate of the embodiment 1-3 according to the number of the colonies of a blank group. The results of the experiment are shown in table 1.
TABLE 1 in vitro test of antibacterial effect of antibacterial medical dressing (antibacterial rate,%)
| Example 1 | Example 2 | Example 3 | |
| Escherichia coli | 98.5％ | 99.5％ | 100％ |
| Staphylococcus aureus | 100％ | 98.6％ | 100％ |
| Candida albicans | 98.8％ | 100％ | 98.9％ |
| Streptococcus pyogenes | 97.6％ | 99.5％ | 100％ |
| Pseudomonas aeruginosa | 99.2％ | 100％ | 99.6％ |
The test results in table 1 show that the antibacterial medical dressing has a good antibacterial effect in vitro, has a killing rate of more than 97% on escherichia coli, staphylococcus aureus, candida albicans, streptococcus pyogenes and pseudomonas aeruginosa, and can effectively inhibit the growth of microorganisms and reduce the incidence rate of wound infection.
Wound healing test
20 Wistar rats were divided into 4 groups of 5 rats each corresponding to example 3 and comparative examples 1-3, respectively. Each rat was anesthetized with 1.5ml/kg by intraperitoneal injection of 10% chloral hydrate, and after removal of the dorsal hairs, the skin was disinfected with 75% ethanol and iodine III. A1 cm area was cut from the dorsal defeathering area of each rat2Full thickness wounds of size. The dressing is respectively coated on each rat, the dressing is locally changed at the wound every day, and the wound healing conditions of the rats are observed on the 2 nd, 4 th, 6 th and 8 th days respectively. The size of the wound is traced by using semitransparent weighing paper, the wound is accurately weighed, and the weight of the weighing paper in unit area is converted into the wound area. The wound healing rate (postoperative wound area-nth wound area)/postoperative wound area × 100%, and the experimental results are shown in table 2.
Table 2 healing promoting effect of antibacterial medical dressing on rat back wound
The test results in table 2 show that the antibacterial medical dressing has an obvious healing promoting effect on the back wound of a rat. Through contrast tests, the combination of three active ingredients, namely vascular endothelial growth factor, silk fibroin and alginate, has a synergistic effect in promoting the rapid growth of granulation tissues of a wound surface compared with the absence of a certain active ingredient, can obviously shorten the healing time of the wound surface, and the healing rate of the wound surface can be close to 100% in 8 days, so that the purpose of rapid healing of the wound is realized. The medical dressing has definite curative effect and safe and reliable use, can be used for wounds such as surgical wounds, burns, scalds and the like, plays the roles of analgesia, hemostasis, inflammation diminishing and antibiosis, does not cause stimulation to skin, has quick and simple preparation method and has wide practical application value.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications or equivalents may be made to the technical solution without departing from the principle of the present invention, and these modifications or equivalents should also be regarded as the protection scope of the present invention.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113616849A (en) * | 2021-08-16 | 2021-11-09 | 牡丹江医学院 | A kind of liquid dressing for external wound care and preparation method thereof |
| CN113679883A (en) * | 2021-09-17 | 2021-11-23 | 吉林省七维生物科技有限公司 | Preparation method of gel dressing containing silk fibroin |
| CN114028608A (en) * | 2021-12-07 | 2022-02-11 | 佳木斯大学 | Rapid hemostatic dressing for trauma nursing and preparation method thereof |
| CN114631934A (en) * | 2022-03-14 | 2022-06-17 | 中国人民解放军联勤保障部队第九二〇医院 | Dressing capable of creating hypoxia microenvironment to promote early healing of acute wound |
| CN118949119A (en) * | 2024-10-11 | 2024-11-15 | 皖南医学院 | A kind of medical cosmetic wound dressing and preparation method thereof |
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