CN111096368A - Method for splitting nutrient substances of soybean milk powder - Google Patents
Method for splitting nutrient substances of soybean milk powder Download PDFInfo
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- CN111096368A CN111096368A CN201911298500.7A CN201911298500A CN111096368A CN 111096368 A CN111096368 A CN 111096368A CN 201911298500 A CN201911298500 A CN 201911298500A CN 111096368 A CN111096368 A CN 111096368A
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- milk powder
- soybean milk
- soybean
- nutrient substances
- sodium alginate
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C11/00—Milk substitutes, e.g. coffee whitener compositions
- A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
- A23C11/10—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
- A23C11/103—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C11/00—Milk substitutes, e.g. coffee whitener compositions
- A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
- A23C11/10—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
- A23C11/103—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
- A23C11/106—Addition of, or treatment with, microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C7/00—Other dairy technology
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Microbiology (AREA)
- Beans For Foods Or Fodder (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for splitting nutrient substances of soybean milk powder, which comprises the following steps: step one, pretreatment of soybean milk powder: emulsifying soybean milk powder, and then adopting60CorTreating with radiation source, wherein the total radiation dose is 3.5-4.5kGy, and the radiation time is 10-20 min; step two, treating soybean germ cells: adding the soybean milk powder pretreated in the step one into the nutrient solution, and then adding the soybean germ cells, wherein the reaction temperature is 33-37 ℃, and the reaction time is 25-35 min. The soybean milk powder is pretreated and emulsified firstly, the purpose of magnetic sodium alginate added in the emulsification process is to realize more complete emulsification, and the emulsification reaction is more sufficient by matching magnetization treatment, so that the soybean milk powder can be more easily split by soybean germ cells and yeast cells by matching with a sodium maleate solution.
Description
Technical Field
The invention relates to the technical field of nutrient substance resolution, in particular to a method for resolving nutrient substances of soybean milk powder.
Background
The milk powder is prepared from milk of cow by sterilizing, defatting, dewatering, and drying. It may also be a product produced using the milk of other animals such as goats. The milk powder can be prepared into modified milk powder, common milk powder, skimmed milk powder and the like according to different production processes and different additive materials, and can be infant milk powder, children milk powder, adult milk powder, milk powder for pregnant women, old people milk powder, special animal milk powder such as cats, dogs and the like according to different using objects. Milk powder is also called milk powder as another name. The milk powder is a food which is prepared by using fresh milk as a raw material, removing almost all water in the milk by a freezing or heating method, drying and adding a proper amount of white granulated sugar. The milk powder is easy to be mixed, convenient to carry and rich in nutrition. The instant milk powder has larger and more loose particles than common milk powder, and can be dissolved quickly by using warm water. Substitutes in animal proteins, also known as surrogate proteins, are of great interest. Thus, many plant milks have emerged. Vegetable milks produced from vegetable ingredients can be used as a substitute for animal derived milk. They overcome and avoid CMPA. The vegetable milk is free of casein, lactose and cholesterol, is rich in vitamins and minerals, and is also rich in essential fatty acids with low content of saturated fatty acids. Some plant milks also contain higher levels of dietary fiber.
The prior art is not mature in the resolution of the nutrient substances of the soybean milk powder and has not good separation effect on protein, grease and carbohydrate, so that further improvement treatment is still needed.
Disclosure of Invention
The invention aims to provide a method for splitting nutrient substances of soybean milk powder, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for resolving soybean milk powder nutrient substances comprises the following steps:
step one, pretreatment of soybean milk powder: emulsifying soybean milk powder, and then adopting60CorTreating with radiation source, wherein the total radiation dose is 3.5-4.5kGy, and the radiation time is 10-20 min;
step two, treating soybean germ cells: adding the soybean milk powder pretreated in the step one into a nutrient solution, and then adding soybean germ cells, wherein the reaction temperature is 33-37 ℃, and the reaction time is 25-35 min;
step three, splitting and strengthening treatment: continuously reacting the soybean milk powder treated by the soybean germ cells in the step two for 20-30min by adopting the strengthening treatment liquid, wherein the reaction temperature is 40-45 ℃;
step four, processing the yeast cells: mixing the soybean milk powder treated in the third step with yeast cells, reacting at 35 ℃ for 20-30min, adding glucose solution during the reaction, and finishing the reaction.
Preferably, the soybean milk powder is emulsified by the following specific steps: stirring the soybean milk powder in deionized water for 20-30min at the stirring speed of 300-500r/min, adding magnetic sodium alginate accounting for 20-30% of the total amount of the soybean milk powder, magnetizing in a magnetizing tank, centrifuging, and drying.
Preferably, a sodium maleate solution with the mass fraction of 0.25-0.35% is also added before the magnetic sodium alginate is added.
Preferably, the preparation method of the magnetic sodium alginate comprises the steps of adding ferroferric oxide into a sodium alginate solution, and then adding calcium chloride to perform solidification for 20-25min to obtain the magnetic sodium alginate.
Preferably, the specific conditions of the magnetization treatment are that the frequency of an electric field is 25-35Hz, the pulse width is 20-30us, the strength of a battery is 30-40v/m, and the action time of the battery is 15-25 min.
Preferably, the specific conditions of the magnetization treatment are that the frequency of an electric field is 30Hz, the pulse width is 25us, the strength of a battery is 35v/m, and the action time of the battery is 20 min.
Preferably, the preparation method of the nutrient solution in the second step is to add the radish juice into the glucose solution, then add the sodium sulfate and the sodium molybdate, and then stir at the rotation speed of 300-350r/min for 20-30min to obtain the nutrient solution.
Preferably, the strengthening treatment liquid in the third step comprises the following raw materials in parts by weight: 10-20 parts of ethyl maltol and 15-25 parts of ethylene diamine tetraacetic acid.
Preferably, the strengthening treatment liquid in the third step comprises the following raw materials in parts by weight: 15 parts of ethyl maltol and 20 parts of ethylene diamine tetraacetic acid.
Compared with the prior art, the invention has the following beneficial effects:
the soybean milk powder is pretreated, the pretreatment is firstly emulsified, the magnetic sodium alginate added in the emulsification process aims to realize more complete emulsification, and the magnetic treatment is matched, so that the emulsification reaction is more sufficient, the soybean milk powder can be more easily split by soybean germ cells and yeast cells by matching with a sodium maleate solution, the separation of protein, grease, cellulose and the like is firstly completed by the soybean germ cells, then macromolecular protein is further hydrolyzed by the yeast cells, so that the protein content and the nutrient components are improved, the splitting strengthening treatment is adopted in the treatment of the soybean germ cells and the yeast cells, the splitting of the nutrient components is strengthened, and the splitting efficiency is improved.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the method for separating the nutrient substances of the soybean milk powder comprises the following steps:
step one, pretreatment of soybean milk powder: emulsifying soybean milk powder, and then adopting60CorTreating with a radiation source, wherein the total radiation dose is 3.5kGy, and the radiation lasts for 10 min;
step two, treating soybean germ cells: adding the soybean milk powder pretreated in the step one into a nutrient solution, and then adding soybean germ cells, wherein the reaction temperature is 33 ℃, and the reaction time is 25 min;
step three, splitting and strengthening treatment: continuously reacting the soybean milk powder treated by the soybean germ cells in the step two for 20-30min by adopting the strengthening treatment liquid, wherein the reaction temperature is 40 ℃;
step four, processing the yeast cells: mixing the soybean milk powder treated in the third step with yeast cells, then placing the mixture at 35 ℃ for reaction for 20min, adding a glucose solution into the mixture during the reaction, and finishing the reaction.
The specific steps of the soybean milk powder emulsification of the embodiment are as follows: placing soybean milk powder in deionized water, stirring for 20min at a stirring speed of 300r/min, adding magnetic sodium alginate in an amount of 20% of the total amount of the soybean milk powder, magnetizing in a magnetizing tank, centrifuging, and drying.
The sodium alginate solution with the mass fraction of 0.25% is also added before the addition of the magnetic sodium alginate.
The preparation method of the magnetic sodium alginate in the embodiment includes adding ferroferric oxide into a sodium alginate solution, and then adding calcium chloride to perform solidification for 20min to obtain the magnetic sodium alginate.
The specific conditions of the magnetization treatment in this example were that the electric field frequency was 25Hz, the pulse width was 20us, the battery strength was 30v/m, and the battery operation time was 15 min.
In the second step of this example, the preparation method of the nutrient solution includes adding the radish juice into the glucose solution, then adding the sodium sulfate and the sodium molybdate, and then stirring at a rotation speed of 300r/min for 20min to obtain the nutrient solution.
The strengthening treatment liquid in the third step of the embodiment comprises the following raw materials in parts by weight: 10 parts of ethyl maltol and 15 parts of ethylene diamine tetraacetic acid.
Example 2:
the method for separating the nutrient substances of the soybean milk powder comprises the following steps:
step one, pretreatment of soybean milk powder: emulsifying soybean milk powder, and then adopting60CorTreating with a radiation source, wherein the total radiation dose is 4.5kGy, and the radiation is carried out for 20 min;
step two, treating soybean germ cells: adding the soybean milk powder pretreated in the step one into a nutrient solution, and then adding soybean germ cells, wherein the reaction temperature is 37 ℃, and the reaction time is 35 min;
step three, splitting and strengthening treatment: continuously reacting the soybean milk powder treated by the soybean germ cells in the step two for 30min by adopting the strengthening treatment liquid, wherein the reaction temperature is 45 ℃;
step four, processing the yeast cells: mixing the soybean milk powder treated in the third step with yeast cells, then placing the mixture at 35 ℃ for reaction for 30min, adding a glucose solution into the mixture during the reaction, and finishing the reaction.
The specific steps of the soybean milk powder emulsification of the embodiment are as follows: placing soybean milk powder in deionized water, stirring for 30min at a stirring speed of 500r/min, adding 30% of magnetic sodium alginate, magnetizing in a magnetizing tank, centrifuging, and drying.
The sodium maleate solution with the mass fraction of 0.35% is also added before the magnetic sodium alginate of the embodiment is added.
The preparation method of the magnetic sodium alginate in the embodiment includes adding ferroferric oxide into a sodium alginate solution, and then adding calcium chloride to perform solidification for 25min to obtain the magnetic sodium alginate.
The specific conditions of the magnetization treatment in this example were an electric field frequency of 35Hz, a pulse width of 30us, a battery strength of 40v/m, and a battery operation time of 25 min.
In the second step of this example, the preparation method of the nutrient solution includes adding the radish juice into the glucose solution, then adding the sodium sulfate and the sodium molybdate, and then stirring at a rotation speed of 350r/min for 30min to obtain the nutrient solution.
The strengthening treatment liquid in the third step of the embodiment comprises the following raw materials in parts by weight: 20 parts of ethyl maltol and 25 parts of ethylene diamine tetraacetic acid.
Example 3:
the method for separating the nutrient substances of the soybean milk powder comprises the following steps:
step one, pretreatment of soybean milk powder: emulsifying soybean milk powder, and then adopting60CorTreating with a radiation source, wherein the total radiation dose is 4.0kGy, and the radiation is carried out for 15 min;
step two, treating soybean germ cells: adding the soybean milk powder pretreated in the step one into a nutrient solution, and then adding soybean germ cells, wherein the reaction temperature is 35 ℃, and the reaction time is 30 min;
step three, splitting and strengthening treatment: continuously reacting the soybean milk powder treated by the soybean germ cells in the step two for 25min by adopting the strengthening treatment liquid, wherein the reaction temperature is 42.5 ℃;
step four, processing the yeast cells: mixing the soybean milk powder treated in the third step with yeast cells, then placing the mixture at 35 ℃ for reaction for 25min, adding a glucose solution into the mixture during the reaction, and finishing the reaction.
The specific steps of the soybean milk powder emulsification of the embodiment are as follows: placing soybean milk powder in deionized water, stirring for 25min at a stirring speed of 400r/min, adding 25% of magnetic sodium alginate, magnetizing in a magnetizing tank, centrifuging, and drying.
The sodium alginate solution with the mass fraction of 0.30% is also added before the addition of the magnetic sodium alginate.
The preparation method of the magnetic sodium alginate in the embodiment includes adding ferroferric oxide into a sodium alginate solution, and then adding calcium chloride to perform solidification for 22.5min to obtain the magnetic sodium alginate.
The specific conditions of the magnetization treatment in this example were an electric field frequency of 30Hz, a pulse width of 25us, a battery strength of 35v/m, and a battery operation time of 20 min.
In the second step of this example, the preparation method of the nutrient solution includes adding the radish juice into the glucose solution, then adding the sodium sulfate and the sodium molybdate, and then stirring at 325r/min for 25min to obtain the nutrient solution.
The strengthening treatment liquid in the third step of the embodiment comprises the following raw materials in parts by weight: 15 parts of ethyl maltol and 20 parts of ethylene diamine tetraacetic acid.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (9)
1. A method for splitting nutrients of soybean milk powder is characterized by comprising the following steps:
step one, pretreatment of soybean milk powder: emulsifying soybean milk powder, and then adopting60CorTreating with radiation source, wherein the total radiation dose is 3.5-4.5kGy, and the radiation time is 10-20 min;
step two, treating soybean germ cells: adding the soybean milk powder pretreated in the step one into a nutrient solution, and then adding soybean germ cells, wherein the reaction temperature is 33-37 ℃, and the reaction time is 25-35 min;
step three, splitting and strengthening treatment: continuously reacting the soybean milk powder treated by the soybean germ cells in the step two for 20-30min by adopting the strengthening treatment liquid, wherein the reaction temperature is 40-45 ℃;
step four, processing the yeast cells: mixing the soybean milk powder treated in the third step with yeast cells, reacting at 35 ℃ for 20-30min, adding glucose solution during the reaction, and finishing the reaction.
2. The method for splitting the nutrient substances of the soybean milk powder according to claim 1, wherein the specific steps of the soybean milk powder emulsification are as follows: stirring the soybean milk powder in deionized water for 20-30min at the stirring speed of 300-500r/min, adding magnetic sodium alginate accounting for 20-30% of the total amount of the soybean milk powder, magnetizing in a magnetizing tank, centrifuging, and drying.
3. The method for resolving the nutrient substances of the soybean milk powder as claimed in claim 2, wherein a 0.25-0.35% sodium maleate solution is added before the magnetic sodium alginate is added.
4. The method for resolving the nutrient substances of the soybean milk powder according to claim 1, wherein the preparation method of the magnetic sodium alginate comprises the steps of adding ferroferric oxide into a sodium alginate solution, then adding calcium chloride, and solidifying for 20-25min to obtain the magnetic sodium alginate.
5. The method for resolving soybean milk powder nutrient substances as claimed in claim 2, wherein the specific conditions of the magnetization treatment are that the electric field frequency is 25-35Hz, the pulse width is 20-30us, the battery intensity is 30-40v/m, and the battery action time is 15-25 min.
6. The method for resolving soybean milk powder nutrient substances as claimed in claim 5, wherein the specific conditions of the magnetization treatment are that the electric field frequency is 30Hz, the pulse width is 25us, the battery intensity is 35v/m, and the battery action time is 20 min.
7. The method for resolving soybean milk powder nutrient substances as claimed in claim 1, wherein the nutrient solution in the second step is prepared by adding radish juice into glucose solution, then adding sodium sulfate and sodium molybdate, and then stirring at a rotation speed of 300-350r/min for 20-30min to obtain the nutrient solution.
8. The method for resolving the nutrient substances of the soybean milk powder as claimed in claim 1, wherein the strengthening treatment liquid in the third step comprises the following raw materials in parts by weight: 10-20 parts of ethyl maltol and 15-25 parts of ethylene diamine tetraacetic acid.
9. The method for resolving the nutrient substances of the soybean milk powder as claimed in claim 8, wherein the strengthening treatment liquid in the third step comprises the following raw materials in parts by weight: 15 parts of ethyl maltol and 20 parts of ethylene diamine tetraacetic acid.
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Inventor after: Gu Ting Inventor after: Wen Chunlan Inventor before: Gu Ting |
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Effective date of registration: 20220316 Address after: Room 409 and 412, building C, Caohu science and Technology Park, Xijiao University, No. 1, Guantang Road, Caohu street, Xiangcheng District, Suzhou, Jiangsu 215100 Applicant after: Suzhou herneng Biotechnology Co.,Ltd. Address before: 730000 403, No. 73, Zhongshan forest, Chengguan District, Lanzhou City, Gansu Province Applicant before: Gu Ting |
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Application publication date: 20200505 |