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CN111094569A - 光控性病毒蛋白质、其基因及包含该基因的病毒载体 - Google Patents

光控性病毒蛋白质、其基因及包含该基因的病毒载体 Download PDF

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CN111094569A
CN111094569A CN201880046636.6A CN201880046636A CN111094569A CN 111094569 A CN111094569 A CN 111094569A CN 201880046636 A CN201880046636 A CN 201880046636A CN 111094569 A CN111094569 A CN 111094569A
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谷宪三朗
佐藤守俊
竹田诚
田原舞乃
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Abstract

本发明的目的在于开发可实现病毒载体活性的控制的病毒载体。通过提供在病毒蛋白质的可导入外源基因的区域以可表达的方式插入有编码光开关蛋白质的基因的来源于RNA病毒的病毒蛋白质基因实现。通过该病毒载体，可通过光照控制病毒蛋白质的酶活性以及基于其的病毒载体的活性。

Description

光控性病毒蛋白质、其基因及包含该基因的病毒载体
技术领域
本发明涉及通过光照控制病毒蛋白质的酶活性的技术。
背景技术
借助于生物技术的进步，使用病毒载体的基因导入技术得已确立，正在进行着针对以腺苷脱氨酶缺乏症等严重的遗传疾病、癌症、艾滋病为代表的各种疾病的基因治疗的研究。作为基因导入技术，大致分为非病毒的导入法和使用病毒的导入法。但是，非病毒的转导技术一般存在导入效率低的倾向，所导入的基因的表达效率也低。基因治疗中，采用使用病毒的基因导入技术，大量的病毒载体正在开发中。
病毒载体可利用病毒原来具有的感染性，导入外源基因。通过在启动子控制下配置外源基因，可在感染细胞中实现外源基因的表达。作为用作这样的病毒载体的病毒，可利用各种病毒，例如以整合入基因组为目的，广泛采用逆转录病毒，以暂时性的基因表达为目的，广泛采用腺病毒或腺伴随病毒。
然而，使用至今为止的病毒载体的情况下，未开发感染后控制病毒载体的活性的方法。使用以整合入基因组为目的的逆转录病毒的情况下，向基因组的整合完成后，病毒也残存，会引发副作用。此外，以暂时性的基因表达为目的使用腺病毒或腺伴随病毒的情况下，也被指出体内的各种组织中残存病毒，还存在与已感染人并处于潜伏状态的病毒之间发生载体拯救(vector rescue)的危险性。
现有技术文献
非专利文献
非专利文献1:J.General virology(1990),71,1153-1162；J.General virology(1997),78,571-576
非专利文献2:Virology(2010)vol.406p.212-227
非专利文献3:J.Virology(2006)vol.80,No.8,p.4122-4134
非专利文献4:J.Virology(2002)vol.76,No.14,7322-7328
非专利文献5:J.Virology(2007)vol.81,No.24,13649-13658
非专利文献6:Vaccine(2010)vol.28,p.1181-1187
非专利文献7:J Virology,(2009)vol.83,p.3549-3555
非专利文献8:Nature Chemical Biology,DOI10.1038/nchembio.2205
非专利文献9:Nature Biotechnology(2015)vol.33,755-760,doi:10.1038/nbt.3245
非专利文献10:Embo J.(2002)vol.21(10):2364-72
非专利文献11:J.Virol.(2006)vol.80:4242-4248
非专利文献12:Virus Res(2005)vol.108:161-165
非专利文献13:J Virological Methods(2006)vol.137:152-155
非专利文献14:Journal of Virological methods(2005)vol.125:35-40
非专利文献15:Journal of Virology(2014)vol.88:11187-11198
非专利文献16:Science,(2012)vol.338,810-814
发明内容
发明所要解决的技术问题
本发明的目的在于开发可实现病毒载体活性的控制的病毒载体。
解决技术问题所采用的技术方案
本发明人发现，通过向包含多个功能结构域的病毒蛋白质的可导入外源基因的区域插入编码光开关蛋白质的基因，可用光照控制病毒蛋白质的酶活性，从而完成了本发明。
因此，本发明涉及下述的发明。
[1]一种病毒蛋白质基因，该病毒蛋白质基因在具有酶活性的病毒蛋白质的可导入外源基因的区域以可表达的方式插入有编码光开关蛋白质的基因，其中，所述光开关蛋白质包含至少2个亚基，编码各亚基的基因介以编码接头的基因连接或者不介以接头而直接连接。
[2]如项目1所述的病毒蛋白质基因，其中，所述接头为10～100个氨基酸残基。
[3]如项目1或2所述的病毒蛋白质基因，其中，所述编码光开关蛋白质的基因为Magnet基因。
[4]如项目3所述的病毒蛋白质基因，其中，该基因可通过照射波长450～490nm的光来调节所述病毒蛋白质的活性。
[5]如项目1～4中的任一项所述的病毒蛋白质基因，其中，具有酶活性的病毒蛋白质为具有聚合酶或蛋白质酶结构域的病毒蛋白质。
[6]如项目1～5中的任一项所述的病毒蛋白质基因，其中，所述病毒为RNA病毒。
[7]如项目1～6中的任一项所述的病毒蛋白质基因，其中，所述病毒蛋白质为RNA依赖性RNA聚合酶。
[8]如项目7所述的病毒蛋白质基因，其中，所述RNA依赖性RNA聚合酶为RNA依赖性RNA聚合酶L蛋白质。
[9]如项目7或8所述的病毒蛋白质基因，其中，所述可导入外源基因的区域为Ln结构域与Lc结构域之间的区域。
[10]如项目1～9中的任一项所述的病毒蛋白质基因，其中，在所述编码光开关蛋白质的基因的上游和/或下游还包含接头。
[11]一种核酸，其中，该核酸编码项目1～10中的任一项所述的病毒蛋白质基因。
[12]一种载体，其中，包含编码项目1～10中的任一项所述的病毒蛋白质基因的核酸。
[13]一种病毒或病毒载体，其中，包含项目1～10中的任一项所述的病毒蛋白质基因。
[14]一种试剂盒，其中，包含项目11所述的核酸、项目11所述的载体、或项目12所述的病毒或病毒载体。
[15]一种细胞，其中，包含项目11所述的核酸。
[16]如项目15所述的细胞，其中，所述细胞为iPS细胞或其后代细胞。
[17]一种通过病毒载体暂时性感染细胞的方法，其中，包括：
使包含导入基因的项目13所述的病毒或病毒载体感染细胞的工序；
在光照下培养已感染的细胞的工序；以及
在无光照条件下培养已感染的细胞的工序。
[18]一种使导入基因表达的方法，其中，包括：
使包含导入基因的项目13所述的病毒或病毒载体感染细胞的工序；以及
在光照下培养已感染的细胞的工序。
[19]一种iPS的制造方法，其中，包括：
使包含选自Oct3/4、Sox2、Klf4、l-Myc(或c-Myc)、Nanog和Lin28的至少1种导入基因的项目13所述的病毒或病毒载体感染体细胞的工序；以及
在光照下培养已感染的细胞的工序。
[20]如项目18或19所述的方法，其中，还包括：在无光照条件下培养已感染的细胞的工序。
发明的效果
本发明的光控性病毒蛋白质可通过光照的有无来控制其酶活性。病毒蛋白质的酶活性有助于该病毒的增殖和感染等活性，所以可通过光照的有无来控制具有这样的光控性病毒蛋白质基因的病毒载体或病毒的活性。
附图说明
图1模式化表示编码麻疹病毒的基因组序列的质粒以及麻疹病毒和重组麻疹病毒的基因组。
图2是表示使本发明的光敏性麻疹病毒载体感染细胞后的对应于感染后天数的EGFP的表达的照片。
图3是表示使本发明的光敏性麻疹病毒载体感染细胞后的对应于感染后天数的蓝色光照射下、红色光照射下或遮光下培养时的麻疹病毒数(PFU/ml)的图表。
图4是表示使本发明的光敏性麻疹病毒载体感染细胞后的对应于感染后天数的蓝色光照射下、红色光照射下或遮光下培养时的麻疹病毒数(PFU/ml)的图表。该图表显示，通过在蓝色光照射下培养至第3天后，遮光下培养，病毒数减少。
图5是表示使本发明的光敏性麻疹病毒载体感染细胞后的对应于感染后天数的蓝色光照射下、红色光照射下或遮光下培养时的麻疹病毒数(PFU/ml)的图表。该图表显示，如果遮光下培养至第7天后，从第7天后在蓝色光照射下培养，则病毒活性恢复并增殖。
图6表示麻疹病毒微型基因组表达质粒的模式图。在EF1α启动子的下游，分别以可表达的方式结合有锤头型核酶、麻疹病毒基因组5'末端序列、纳米荧光素酶、麻疹病毒3'末端序列、HDV核酶。如果该质粒被转染至细胞内，则通过EF1α启动子的作用，RNA被转录，核酶的序列经核酶的作用而被剪切，产生麻疹病毒微型基因组。
图7表示在CAG启动子的下游分别配置有N基因、P基因和L基因的N蛋白表达质粒、P蛋白表达质粒、L(或修饰L)蛋白表达质粒和麻疹病毒微型基因组表达质粒的模式图。这些质粒如果被共转染至细胞，则被翻译而生成N蛋白、P蛋白、L蛋白(或修饰L蛋白)，同时生成麻疹病毒微型基因组。它们在细胞内生成核糖核蛋白复合物，通过L蛋白的RNA依赖性RNA聚合酶的作用，生成mRNA，表达微型基因组内所编码的蛋白质(纳米荧光素酶)。
图8表示与N蛋白表达质粒、P蛋白表达质粒、麻疹病毒微型基因组表达质粒一起共转染L蛋白或修饰L蛋白的情况下的荧光素酶活性。该图表示遮光或蓝色光照射下进行培养的结果。
图9是分别模式化表示在麻疹病毒载体的L基因的位置分别具有L基因的N末端侧与pMag连接而得的基因以及L基因的C末端侧与nMaghigh基因连接而得的基因的病毒载体的图。
图10是在狂犬病病毒的序列上追加EGFP转录单元、以及光开关蛋白质单元(pMag-linker-nMaghigh)、光开关蛋白质单元(pMag-linker-nMag)和直接结合光开关蛋白质单元(pMag-Direct-nMaghigh)而得的序列的模式图。
图11是在风疹病毒的序列中的p150蛋白质的可导入外源基因的区域导入AG1和光开关蛋白质单元(pMag-linker-nMaghigh)而得的序列的模式图。
图12是在风疹病毒的序列中的p150蛋白质的可导入外源基因的区域导入光开关蛋白质单元(pMag-linker-nMaghigh)并进一步用报告基因(纳米荧光素酶)取代结构蛋白质的区域而得的报告基因表达亚基因组复制子的模式图。
图13模式化表示麻疹病毒和重组麻疹病毒的基因组。重组麻疹病毒中所导入的光开关蛋白质单元中，制成了使用26个残基的接头的单元(pMag-linker-nMaghigh)、使连接亚基的接头的长度变为约4倍的单元(pMag-4×linker-nMaghigh)和不使用接头而直接连接的单元(pMag-direct-nMaghigh)。此外，制成了作为光开关蛋白质的亚基使用nMag代替nMaghigh的重组麻疹病毒(pMag-linker-nMag)。
图14是表示使图13中制成的重组麻疹病毒感染培养细胞并在蓝色光照射下或遮光下培养时的麻疹病毒数(PFU/ml)的图表。
图15是表示将图10中制成的重组狂犬病病毒感染培养细胞在蓝色光照射下或遮光下培养时的狂犬病病毒数(PFU/ml)的图表。
图16是表示肿瘤小鼠模型中使肿瘤感染重组麻疹病毒后基于蓝色光照射的肿瘤体积的变迁的图表(A)和表示存活率的图表(B)。
图17表示iPS细胞制造用的麻疹病毒的基因组的模式图。图17B表示通过iPS细胞制造用的病毒载体感染而制成的iPS细胞以及细胞中所表达的EGFP。
具体实施方式
本发明涉及在包含多个功能结构域的病毒蛋白质的可导入外源基因的区域以可表达的方式插入有编码光开关蛋白质的基因的病毒蛋白质基因。在此，本发明的特征在于，光开关蛋白质包含至少2个亚基，编码各亚基的基因介以接头连接。
另一形态中，本发明涉及在包含多个功能结构域的病毒蛋白质的可导入外源基因的区域以可表达的方式插入有编码光开关蛋白质的基因的病毒蛋白质基因。在此，本发明的特征在于，光开关蛋白质包含至少2个亚基，编码各亚基的基因不介以接头而直接连接。
各亚基可在能够发挥所期望的活性的范围内赋予1个或数个氨基酸的置换、缺失和添加。特别是在介以接头或不介以接头连接的部位，亚基中可存在氨基酸的缺失。
病毒根据基因组的种类大致分为RNA病毒和DNA病毒。另外，根据基因组由单链构成还是由双链构成而区分，单链的情况下，还根据正链或负链而细分。本发明中，病毒是指DNA病毒和RNA病毒这两者，但较好是RNA病毒。RNA病毒被国际病毒分类委员会分类为第三组～第六组，本发明中，病毒可涉及属于第三组～第六组的任意组的RNA病毒。特别是从向具有RNA依赖性RNA聚合酶作用的L蛋白质导入光开关蛋白质的观点来看，本发明的病毒较好是涉及属于单链RNA负链(第五组)的病毒。此外，从向p150导入光开关蛋白质的观点来看，本发明的病毒可涉及属于单链RNA正链(第四组)的病毒。
作为属于国际病毒分类第五组的具有单链RNA负链的基因组的病毒，可例举单股反链病毒目的副黏液病毒科、炮弹病毒科、丝状病毒科、玻那病毒科和无归属目的正粘病毒科、沙粒病毒科、布尼亚病毒科的病毒。这些病毒的基因组复制需要RNA依赖性RNA蛋白质，具有L蛋白质或其家族。因此，可在这些病毒的L蛋白质或其家族蛋白质的可导入外源基因的区域导入光开关蛋白质。已知特别是属于炮弹病毒科的水疱性口炎病毒(Vesicularstomatitis virus)、狂犬病病毒(Rabies virus)以及属于副黏液病毒科的麻疹病毒(Measles virus)、仙台病毒(Sendai virus)、新城疫病毒(Newcastle disease virus)、犬瘟热病毒(Canine distemper virus)、海豹瘟热病毒(Phocine distemper virus)、牛瘟病毒(Rinderpest virus)的L蛋白质分别具有非常高的同源性(非专利文献1:J.Generalvirology(1990),71,1153-1162；J.General virology(1997),78,571-576)。
作为属于国际病毒分类第四组的具有单链RNA正链的基因组的病毒，可例举网巢病毒目的冠状病毒科、动脉炎病毒科、属于微小核糖核酸病毒目的微小核糖核酸病毒科、无归属目的披膜病毒科、黄病毒科、杯状病毒科、星状病毒科、戊型肝炎病毒科的病毒。这些病毒中，较好是属于具有p150蛋白质和p90蛋白质的披膜病毒科风疹病毒属或者具有nsP1、nsP2、nsP3、nsP4的披膜病毒科甲病毒属的病毒，特别好是风疹病毒属的风疹病毒。此外，作为以具有单链RNA正链且具有逆转录酶为特征的国际病毒分类第六组，可例举无归属目的逆转录病毒科的病毒。
DNA病毒中，发现有最大具有30万碱基的基因组尺寸的病毒。另一方面，RNA病毒具有比DNA病毒小的尺寸的基因组，平均具有1万碱基、至多3万碱基左右的基因组尺寸。因此，RNA病毒在自身的病毒基因组上所编码的病毒蛋白质的数量也较少。例如，被分类为单股反链病毒目副黏液病毒科麻疹病毒属的麻疹病毒中，具有约16k碱基的负链的单链RNA基因组，自3'末端起，由被命名为N、P、M、F、H、L的6个基因构成。此外，被分类为披膜病毒科风疹病毒属的风疹病毒为约12k碱基的正链的单链RNA，自3'末端起，存在被命名为p150、p90、衣壳、E2、E1的基因。
病毒蛋白质主要大致分为成为病毒粒子的构成成分的结构蛋白质以及在感染细胞内表达而在病毒的RNA合成和抗免疫作用中发挥作用、但未整合入病毒粒子中的非结构蛋白质。本发明中的“具有酶活性的病毒蛋白质”可以是结构蛋白质，也可以是非结构蛋白质。作为具有酶活性的病毒蛋白质，可例举聚合酶、蛋白质酶、帽甲基转移酶、解旋酶、整合酶、神经氨酸酶等。具有酶活性的蛋白质通常是该病毒的感染和增殖所必需的蛋白质，如果其功能被抑制，则病毒的活性下降，有可能会失活。具有酶活性的蛋白质大多具有1个或多个功能结构域。作为功能结构域，可例举聚合酶结构域和蛋白质酶等直接影响酶活性的结构域以及对酶活性结构域起到辅助作用的结构域。有时在这样的结构域间具有铰链结构域和功能未知(或不发挥功能)的结构域，可在这样的结构域间导入外源基因。将即使导入外源基因也依然可维持具有酶活性的蛋白质的活性的区域称为可导入外源基因的区域。
作为具有可导入外源基因的区域的具酶活性的蛋白质，可例举例如麻疹病毒的L蛋白质和风疹病毒的p150(非专利文献2:Virology 406(2010)p.212-227)、辛德比斯病毒的非结构蛋白质P3(非专利文献3:J.Virology(2006),vol.80,No.8,p.4122-4134)，但并不仅限于这些例子。如果是本领域技术人员，可任意地选择具有可导入外源基因的区域的病毒蛋白质基因。能够导入可导入外源基因的区域的基因尺寸根据包含可导入外源基因的区域的病毒蛋白质的种类而不同。一般来说，即使是可导入外源基因的区域，可导入的基因也根据其尺寸和翻译后的立体结构而不同。可导入的基因的尺寸的上限可在维持病毒蛋白质的活性的范围内任意选择，例如为1000个氨基酸残基以下，较好是500个氨基酸残基以下，更好是400个氨基酸残基以下。可导入的基因的尺寸的下限无需特别限定。
L蛋白质是属于单股反链病毒目的病毒共有的RNA依赖性RNA聚合酶。单股反链病毒目包括5个家族，即使是属于不同家族的单股反链病毒，在L蛋白质的结构上也存在许多共同点，具有RNA依赖性RNA聚合酶结构域、加帽结构域、连接结构域、甲基转移酶结构域、C末端结构域。将单股反链病毒目的L蛋白质内氨基酸序列的同源性非常高的区域称为结构域I、II、III、IV、V、VI。单股反链病毒的L蛋白质之间还具有氨基酸序列的高同源性，同源性高的区域被称为D1～D3结构域。这些结构域通过可变铰链结构域(H1和H2)连接。在此，H2结构域成为可导入外源基因的区域。因此，L蛋白质分为包含D1、H1及D2的N末端结构域(Ln)和包含D3结构域的C末端结构域(Lc)，可在Ln与Lc之间导入外源基因。更具体来说，麻疹病毒的L蛋白质的情况下，可将从起始密码子至1707残基作为Ln结构域，从1708残基至2181残基作为Lc结构域。已知能够向该可导入外源基因的区域在不影响L蛋白质的活性的情况下导入250残基左右的外源多肽(非专利文献4:J.Virology(2002)vol.76,No.14,7322-7328；非专利文献5:J.Virology(2007)vol.81,No.24,13649-13658)，但只要不会失去L蛋白质的活性，可导入任意尺寸的多肽。理论上不进行限定，但如果可使Ln结构域与Lc结构域存在于邻近的位置，则L蛋白质的活性得到发挥，所以即使是800残基以上的多肽也可导入，作为上限，较好是800残基以下，更好是600残基以下，进一步更好是400残基以下，再进一步更好是300残基以下。
p150蛋白质在氨基末端由甲基转移酶结构域、大结构域(macrodomain，X结构域)和蛋白质酶结构域构成。X结构域的上游存在脯氨酸铰链(PH)结构域和功能未知的Y结构域。已知包含PH结构域的Q结构域具有可导入外源基因的区域，能够在不影响p150蛋白质的活性的情况下导入250残基左右的外源多肽(非专利文献6:Vaccine 28(2010),p.1181-1187；非专利文献7:J Virology,2009,vol.83,p.3549-3555)。在可发挥p150的活性的范围内，即使是800残基以上的多肽也可导入，作为上限，较好是800残基以下，更好是600残基以下，进一步更好是400残基以下，再进一步更好是300残基以下。
光开关蛋白质是指光敏性蛋白质中，可通过具有特定波长的光的照射，控制自身或其他蛋白质的活性的蛋白质。作为光敏性蛋白质，已知以视紫质和光受体蛋白质CRY2为代表的各种蛋白质，其中特别好是分为2个以上的亚基且看通过光照控制亚基间的聚集和解离的蛋白质。作为由2个以上的亚基构成的光开关蛋白质基因，可使用例如Magnet基因(pMag(序列编号1)-nMagHigh(序列编号2)、Dronpa基因(DPK(序列编号3)-DPN(序列编号4))(非专利文献16:X.X.Zhou,K.Chung,A.J.Lam and M.Z.Lin,Science,338,810-814(2012))、Cry2(序列编号5)-CIB1N(序列编号6)系、以及它们的修饰基因，但并不仅限于这些例子。从整合于L基因的观点来看，较好是Magnet基因(pMag-nMag)。
照射的光的波长可根据使用的光开关蛋白质的种类适当选择。使用Magnet基因(pMag-nMag)的情况下，可使用450～490nm的波长；使用Dronpa基因(DPK-DPN)的情况下，可使用390～500nm的波长；使用Cry2-CIB1N基因的情况下，可使用450～490nm的波长。从控制生物体内的光开关蛋白质的开与关的观点来看，较好是生物体内的透射性高的波长，例如450～900nm的波长，更好是650～900nm。光开关蛋白质可为改变敏感性的波长区域而任意地进行修饰。因此，如果是本领域技术人员，可修饰公知的光开关蛋白质，根据本发明的使用方式使其达到优选的吸收波长。
由2个以上的亚基构成的光开关蛋白质通常具有独立游离的亚基在特定波长的光的照射下聚集的性质。利用该性质，设计出了通过将光开关蛋白质与Cre-loxP系统组合，可用光控制DNA重组反应的系统(非特許文献8:Nature Chemical Biology,DOI10.1038/nchembio.2205)。具体为将作为DNA重组酶的Cre分为两部分，在N末端侧(CreN)和C末端侧(CreC)分别连接作为光开关蛋白质的Magnet基因的亚基(pMag和nMag)，独立进行表达的系统。CreN-pMag和CreC-nMag在无光照的条件下分别作为游离亚基存在，但在蓝色光照射下pMag与nMag聚集，发挥Cre的酶活性。同样地，设计出了通过将作为光开关蛋白质的Magnet基因与Crisper-Cas9系统组合，可用光控制DNA重组反应的系统(非特許文献9:NatureBiotechnology 33,755-760(2015)doi:10.1038/nbt.3245)。用作Magnet基因的pMag亚基(序列编号1)与nMag亚基(序列编号7)分别具有序列编号1的氨基酸序列和序列编号2的氨基酸序列，作为游离的蛋白质表达，在蓝色光照射下聚集。已知聚集速度不同的修饰型基因，也可使用经修饰的Magnet基因。作为pMag亚基(序列编号1)的修饰型基因，已知pMagFast1(序列编号76)、pMagFast2(序列编号77)等。此外，作为nMag亚基(序列编号7)的修饰型基因，已知nMagHigh(序列编号2)等。
本发明中，由2个以上的亚基构成的光开关蛋白质在亚基间介以接头连接。亚基数为n的情况下，亚基间的接头存在n-1个。各接头的长度可根据既发挥光开关基因的功能又发挥病毒蛋白质的功能的观点任意地进行选择。将光开关基因整合于可导入外源基因的区域的情况下，只要可实现病毒拯救，可通过调整接头残基数来调节光开关基因的功能。作为其理由，是因为可实现病毒拯救可认为指光照下光开关蛋白质的亚基聚集，可维持聚集状态下的病毒蛋白质的活性，接头的长度不影响聚集状态，而未聚集状态(非光照条件)下，如果以一定的长度延伸接头，能够降低病毒蛋白质的活性。
接头残基数的上限能够根据可导入外源基因的区域中的导入基因的尺寸的上限和导入的光开关蛋白质的亚基的尺寸适当选择，例如较好是100残基以下，更好是50残基以下，进一步更好是30残基以下。从允许亚基的游离和聚集的观点来看，接头残基数的下限较好是10残基以上，更好是15残基以上，进一步更好是20残基以上。接头的序列可以是任意的序列，较好是不形成β折叠和α螺旋等二级结构。因此，接头中的甘氨酸的含量较好是25％以上，更好是40％以上，进一步更好是50％以上。
此外，光开关蛋白质与病毒蛋白质之间可再介以接头连接，也可直接连接。可将N末端侧的亚基与病毒蛋白质之间的接头称为N-接头(或上游接头)，C末端侧的亚基与病毒蛋白质之间的接头称为C-接头(或下游接头)。N-接头和c-接头的残基的上限和下限可根据导入基因的尺寸的上限和导入的光开关蛋白质的亚基的尺寸适当选择。例如，较好是50残基以下，更好是30残基以下，进一步更好是15残基以下。可包含用于检测是否能够导入外源基因的标签来代替接头或与接头同时包含。
本发明的一种形式中，涉及编码以可表达的方式插入有编码本发明所涉及的光开关蛋白质的基因的编码病毒蛋白质基因的核酸。“编码基因的核酸”中，除原本的核酸序列之外，还通过简并而包含编码同一基因的核酸。本发明的另一种形式中，还涉及包含所述核酸的质粒载体和病毒载体。
除编码病毒基因的核酸之外，本发明的病毒载体还可包含编码任意的外源基因的核酸。本发明的病毒载体可通过光照进行局部的病毒载体的激活，所以给予病毒载体后，可通过光照进行部位特异性的病毒载体的激活，表达外源基因。光可从体外照射，也可使用内窥镜从体内照射。可将这样的病毒载体称为光敏性病毒载体。此外，本发明的光敏性病毒可利用该病毒具有的细胞溶解性，特别是肿瘤溶解活性，用于癌症等疾病的治疗。光敏性病毒感染后，可通过对包含作为目标的细胞、特别是癌细胞的区域照射光，部位特异性地引发细胞溶解。
麻疹病毒一般可将多条基因组整合于粒子内(非专利文献10:Emboj.2002 May15,21(10):2364-72)。因此，即使将基因组分为2条或3条，也可进行增殖(非专利文献11:J.Virol.2006；80:4242-4248)。因此，本发明的病毒载体并不一定仅由1条基因组构成，也可根据情况包含2条、3条或更多条数的基因组。
导入光敏性病毒载体的外源基因可根据其目的适当进行选择。例如，用于针对癌症的基因治疗的情况下，也可向癌细胞直接导入抑癌基因(p53、Rb、RCA1等)，能够使淋巴细胞表达T细胞受体(TCR)等的基因，提高肿瘤抗原特异性的细胞杀伤活性。此外，作为针对基因缺陷伴随的遗传病的患者的基因导入治疗，可将能够补足基因缺陷的基因作为外源基因导入病毒载体。作为这样的基因，可使用例如肌肉营养不良症的治疗中所用的抗肌萎缩蛋白、层粘连蛋白a2、肌营养不良蛋白聚糖等。对于为了治疗对象的疾病的治疗而导入的基因，本领域技术人员可适当选择。
除治疗目的以外，还可为了研究目的等而利用本发明的病毒载体。用本发明的病毒载体感染细胞后，通过照射规定的光，可实现外源基因的暂时表达，其后还可通过停止光照，使病毒的活性下降，从细胞清除病毒。iPS细胞的制作需要使体细胞表达OCT3/4、SOX2、Klf4、l-Myc(或c-Myc)等重编程因子。采用以往的病毒载体的实验中，无法控制病毒的活性。这样的话，使用以往的病毒载体的实验中，无法在诱导成iPS细胞后清除病毒。另一方面，导入的因子的一部分还已知为癌基因，所以被指出存在iPS细胞癌症化的危险。通过利用本发明的病毒载体进行基因导入，进行光照，可暂时在细胞中表达1个或多个重编程因子。并且，通过停止光照，可降低病毒活性或使其失活。由此，在诱导iPS细胞后，通过反复传代，可将病毒完全清除。
本发明的另一种形式中，还涉及使用本发明的光敏性病毒载体的使细胞暂时感染病毒载体的方法。更具体来说，该方法包括使细胞感染本发明的光敏性病毒载体的工序、在光的存在下培养已感染的细胞的工序以及在不存在光的条件下培养已感染的细胞的工序。通过在光的存在下进行培养，可激活病毒载体，促进导入基因的基因表达。然后，通过在不存在光的条件下进行培养，可使病毒失活，清除病毒。为了将病毒完全清除，可进行在暗处的传代培养。通过数代的传代培养，可将病毒完全清除。
暂时感染病毒载体的方法可应用于iPS细胞的制造方法。该方法中包括使包含选自Oct3/4、Sox2、Klf4、l-Myc(或c-Myc)、Nanog和Lin28的至少1种导入基因的本发明的光敏性病毒载体感染体细胞的工序以及在光的存在下培养已感染的细胞的工序。通过在光的存在下进行培养，导入基因在细胞内被表达，表达特定的组合、例如Oct3/4、Sox2、Klf4和l-Myc或者Oct3/4、Nanog和Lin28的情况下，被诱导为iPS细胞。对于诱导得到的iPS细胞，可还实施在不存在光的条件下进行培养的工序。通过该工序，感染iPS细胞的病毒载体失活。为了将病毒载体完全清除，可进行在暗处的传代培养。通过数代的传代培养，可将病毒载体完全清除。由此，可制成不含导入基因的iPS细胞。不含导入基因的iPS细胞是指实质上不含为了诱导iPS细胞而导入的导入基因和用于导入该导入基因的病毒载体的iPS细胞或其后代细胞。“实质上不含病毒载体”是指通过例如RT-PCR的方法确认病毒载体的存在时未检出。
本说明书中所提及的所有文献通过引用将其整体纳入本说明书中。
以下说明的本发明的实施例仅用于示例，并不限定本发明的技术范围。本发明的技术范围仅由权利要求书的记载限定。以不脱离本发明的主旨为条件，可进行本发明的变更、例如本发明的构成要件的追加、删除和置换。
实施例1:导入有光开关蛋白质的病毒的制备
重组麻疹病毒生成用质粒的制备
以编码麻疹病毒IC-B株的基因组序列的质粒p(+)MV323为基础，制成了重组麻疹病毒生成用质粒。在麻疹病毒的基因组的前端追加了EGFP转录单元(图1)(序列编号8)。由此，可通过EGFP的表达确认感染了病毒的细胞。向L蛋白基因的可导入外源基因的区域导入作为光应答性基因的DP(序列编号9)、pMag-nMaghigh(序列编号10)、CRY2-CIB1N(序列编号11)和CRY2PHR-CIB1N(序列编号12)的基因，制成修饰L蛋白基因。这些基因导入使用In-fusion试剂盒(克隆泰克公司(Clontech))进行。为了制备该质粒而使用的PCR引物如下所述。导入了这些基因的质粒的序列为p(+)MV323-DPK-DPN(序列编号13)、p(+)MV323-pMag-nMaghigh(序列编号14)、p(+)MV323-CRY2-CIB1N(序列编号15)和p(+)MV323-CRYPHR-CIB1N(序列编号16)。
[表1]
Figure BDA0002364350710000161
重组麻疹病毒的合成
使用X-tremeGENE HP(罗氏公司)向在6孔板生长的BHK/T7-9细胞转染了0.8μg的N蛋白表达质粒(pCITE-IC-N)(序列编号33)、0.6μg的P蛋白表达质粒(pCITE-IC-PΔC)(序列编号34)、1.6μg的L蛋白表达质粒(pCITE-9301B-wtL)(序列编号35)、上述的重组麻疹病毒生成用质粒(5μg)。培养2天后，将BHK/T7-9细胞叠层于Vero/hSLAM细胞，考察了病毒拯救的有无。
[表2]
Figure BDA0002364350710000171
光应答性的确认
使被拯救的重组麻疹病毒以MOI达到0.01的条件感染于Vero/hSLAM细胞。感染后，将感染了整合有光应答性基因的麻疹病毒的细胞分为蓝色LED(峰波长:蓝色470nm)照射组、暗处组、红色LED(峰波长:655nm)照射组进行培养，考察了EGFP的表达的有无。作为光开关蛋白质导入了pMag-nMaghigh的情况下，蓝色LED照射组中可见EGFP的表达，而暗处组中EGFP的表达少(图2)。将该光应答性的病毒称为“Magnet-L光敏性麻疹病毒”。
实施例2:光敏性麻疹病毒的基于光照的活性控制
光敏性麻疹病毒的增殖曲线
使实施例1中获得的Magnet-L光敏性麻疹病毒以MOI达到0.01的条件感染于在6孔板生长的Vero/hSLAM细胞。照射蓝色或红色LED的情况下，在CO2培养箱内，用ISLM-150×150-RB(CCS)照射各颜色的LED(峰波长:红655nm，蓝色470nm)的同时进行培养。以各种时间回收细胞和培养液，测定了增殖的病毒的感染效价(PFU)(图3)。仅蓝色LED照射组中确认了病毒的增殖(图3)。通过用荧光显微镜确认EGFP的荧光，也确认了病毒依存于蓝色LED照射增殖(图2)。实施同样的实验，在蓝色LED照射组中，一部分从第4天停止蓝色LED照射，观察了病毒增殖的变化(图4)。停止蓝色LED的照射后，病毒增殖的停止，效价逐渐降低(图4)。实施同样的实验，将感染了Magnet-L光敏性麻疹病毒的Vero/hSLAM细胞在暗处培养7天，从第7天开始蓝色LED照射。蓝色LED照射开始后，病毒的增殖开始，第10天起确认到高病毒效价(图5)。
实施例3:微型基因组测试
麻疹病毒微型基因组表达质粒的制备
用pEF-DEST51(赛默飞世尔科技公司)的EF1α启动子序列替代pcDNA3(赛默飞世尔科技公司)的CMV启动子区域，制成在该EF1α启动子序列的下游整合有锤头型核酶序列(序列编号42)、麻疹病毒5'末端序列(序列编号43)、nano luc基因序列(普洛麦格公司(Promega社)，来源于pNL3.3)(序列编号44)、麻疹病毒3'末端序列(序列编号45)和HDV核酶序列(序列编号46)的麻疹病毒微型基因组表达质粒(序列编号47)(图6)。如果将该麻疹病毒微型基因组表达质粒转染至细胞内，则通过EF1α启动子的作用，5'末端具有锤头型核酶、3'末端具有HDV核酶的RNA被转录。所转录的RNA的核酶部分通过这些核酶的作用被剪切，生成模拟麻疹病毒基因组的具有nano luc基因的麻疹病毒微型基因组(图6)。
麻疹病毒蛋白表达质粒的制备微型基因组测试
在pCA7质粒(与pCAG-T7质粒相同)的CAG启动子的下游导入麻疹病毒IC-B株的N基因序列，获得N蛋白质表达质粒(pCA7-IC-N:序列编号48)。同样地，在pCA7质粒的CAG启动子的下游导入麻疹病毒IC-B株的P基因序列，获得P蛋白质表达质粒(pCA7-IC-PΔC:序列编号49)。另外，在pCA7质粒的CAG启动子的下游导入麻疹病毒9301B株的L基因序列，获得L蛋白质表达质粒(pCA7-9301b-wtL:序列编号50)。这些质粒按照非专利文献12(Takeda等2005Virus Res 108:161-165)和非专利文献13(Nakatsu等2006J Virological Methods 137:152-155)的记载获得。另外，代替L基因序列，以上述的p(+)MV323-pMagnMaghigh(序列编号14)的修饰L蛋白基因为基础，与上述的方法同样地进行操作，获得修饰L蛋白质(modifiedL)表达质粒(序列编号51)。
[表3]
Figure BDA0002364350710000191
微型基因组测试
将上述的麻疹病毒微型基因组表达质粒(0.03μg)、N蛋白质表达质粒(0.06μg)、P蛋白质表达质粒(0.015μg)、L蛋白质表达质粒或L修饰蛋白质表达质粒(0.06μg)用TransITLT1(米鲁生物科技公司(Mirus))转染于接种在96孔板的293T细胞。转染后的细胞中，生成麻疹病毒微型基因组的同时，表达N蛋白质、P蛋白质和L蛋白质(或L修饰蛋白质)，这些蛋白质全部具备时，形成核糖核蛋白复合物(图7)。核糖核蛋白复合物通过L蛋白的RNA依赖性RNA聚合酶活性转录nano Luc基因，通过细胞内的翻译系统表达nano Luc(图7)。于是，在转染后的细胞中，在暗处或规定波长(蓝色LED照射(470nm))的光照下培养2天后，回收10μl的培养上清，用Nano-Glo荧光素酶测试试剂盒(普洛麦格公司)测定了荧光素酶活性。其结果示于图8。图8中，将表达野生型L蛋白质的情况下的在暗处的荧光素酶活性作为100％。表达以接头序列整合了pMag和nMaghigh的L蛋白质(pMag-nMaghigh)的细胞组中，在暗处(dark)可见荧光素酶活性的显著下降，而蓝色LED照射下(blue)，检出荧光素酶活性(图8)。如实施例2所示，具有以接头序列整合了pMag和nMaghigh的修饰L蛋白质(pMag-nMaghigh)的Magnet-L光敏性麻疹病毒显示可通过蓝色光的照射控制其活性。本实验的微型基因组测试中，也显示可进行修饰L蛋白质的光控制，所述微型基因组测试显示可替代实际采用病毒的试验使用。
实施例4:接头的探讨
利用2条麻疹病毒基因组，尝试了是否可通过不具有接头的pMag、nMaghigh合成光控性的麻疹病毒。具体来说，对于一方的基因组的L基因，制成L基因的N末端侧与pMag连接的病毒载体，而对于另一方的基因组的L基因，制成nMaghigh基因与L基因的C末端侧连接的病毒载体(序列编号74和75，图9)。使这些病毒载体共感染细胞并转录翻译后，如果照射蓝色光，则pMag与nMaghigh聚集，因此被认为生成活性型的L蛋白质，但实际上未能实现病毒拯救。无接头的情况下，通过蓝色光照射也未形成活性型L蛋白，而pMag与nMaghigh介以接头连接于1个蛋白质上，因而被认为可通过蓝色光照射的有无控制活性。
实施例1中实现了病毒拯救，而对于未显示光应答性的修饰L蛋白质，可进行接头的探讨。可实现病毒拯救的情况下，可认为光照下开关蛋白质的亚基聚集，可维持聚集状态下的病毒蛋白质的活性，接头的长度不影响聚集状态，而未聚集状态下，如果延伸接头，达到一定程度时，能够降低病毒蛋白质的活性。因此，通过使用比目前采用的接头更长的接头，可实现光应答性。
实施例5:以狂犬病病毒为基础的光控性病毒载体的制备
重组狂犬病病毒生成用质粒的制备
以编码狂犬病病毒HEP-Flury株的基因组序列的质粒pHEP为基础，制成了重组狂犬病病毒生成用质粒。在狂犬病病毒的基因组的G基因与L基因之间追加了EGFP转录单元(图10)(序列编号52)。由此，可通过EGFP的表达确认感染了病毒的细胞。该质粒按照非专利文献14(Khawplod等2005Journal of Virological methods 125:35-40)的记载获得。向L基因的可导入外源基因的区域导入了光应答性基因。作为所导入的光应答性基因，使用以26残基的接头结合的pMag-linker-nMaghigh(序列编号10)、不介以接头直接结合的pMag-direct-nMaghigh(序列编号78)、以nMag代替nMaghigh的pMag-linker-nMag(序列编号79)，制成修饰L基因。这些基因导入使用In-fusion试剂盒(克隆泰克公司)进行。为了制备该质粒而使用的PCR引物如下所述。导入了这些基因的质粒的序列为pHEP-pMag-linker-nMaghigh(序列编号53)、pHEP-pMag-direct-nMaghigh(序列编号84)、pHEP-pMag-linker-nMag(序列编号85)。
[表4]
引物 序列
正向1 TGTTACCTCCAACACGTGCTACG(序列编号54)
正向2 CAACAAGCTTAAGagcCATACTCTTTATGCCCCCG(序列编号55)
正向3 cgGG<u>CCTTAAG</u>GTATCTCGCAAGGCAGGAT(序列编号56)
反向1 CTCTTAAGCTTGTTGGGGCTGTAATCTC(序列编号57)
反向2 ATACCTTAAGGCCCGCGTAATCTGGGAC(序列编号58)
反向3 ATGCCCAGGTCGGACCG(序列编号59)
重组狂犬病病毒的合成
使用X-tremeGENE HP(罗氏公司)向在6孔板生长的BHK/T7-9细胞分别转染了0.8μg的N蛋白表达质粒(pH-N)(序列编号60)、0.6μg的P蛋白表达质粒(pH-P)(序列编号61)、1.6μg的L蛋白表达质粒(pH-L)(序列编号62)、0.5μg的G蛋白表达质粒(pH-G)(序列编号63)、上述的重组狂犬病病毒生成用质粒pHEP-pMag-linker-nMaghigh(序列编号53)、pHEP-pMag-direct-nMaghigh(序列编号84)和pHEP-pMag-linker-nMag(序列编号85)(5μg)。对BHK/T7-9细胞进行传代的同时持续培养而获得病毒。
光应答性的确认
使被拯救的重组狂犬病病毒以MOI达到0.01的条件感染于BHK细胞。感染后，将感染了整合有光应答性基因的狂犬病病毒的细胞分为蓝色LED(峰波长：蓝色470nm)照射组和暗处组进行培养，考察了EGFP的表达的有无。照射蓝色LED的情况下，作为光应答性基因导入了pMag-linker-nMaghigh(序列编号10)、pMag-direct-nMaghigh(序列编号78)、以及pMag-linker-nMag(序列编号79)的病毒均同等增殖。另一方面，暗处组中，显示按照pMag-linker-nMaghigh(序列编号10)、pMag-direct-nMag(序列编号79)和pMag-linker-nMaghigh(序列编号78)的顺序病毒增殖活性被抑制(图15)。
实施例6：以风疹病毒为基础的光控性病毒载体的制备
重组风疹病毒生成用质粒的制备
以编码风疹病毒Hiroshima株的基因组序列的质粒pHS为基础，制成了重组风疹病毒生成用质粒。向风疹病毒的基因组的P150基因内部的氨基酸编号717的可导入外源基因的区域插入Azami Green 1(AG1)(MBL)(图11)(序列编号64)。该质粒按照非专利文献15(Sakata等2014 Journal of Virology 88：11187-11198)的记载获得。由此，可通过AG1的表达确认感染了病毒的细胞。向存在于P150基因内的另一个可导入外源基因的区域(氨基酸编号1005)导入pMag-nMaghigh(序列编号10)、制成修饰P150基因。这些基因导入使用In-fusion试剂盒(克隆泰克公司)进行。为了制备各质粒而使用的PCR引物如下所述。导入了这些基因的质粒的序列为pHS-717AG1-1005pMag-nMaghigh(序列编号65)。还制成将AG1与nMaghigh的位置交换而得的pHS-717pMag-nMaghigh-1005AG1(序列编号66)。
[表5]
引物 序列
717正向 CGGCCAGTCCaagcttagcCATACTCTTTATGCCCCCGGT(序列编号67)
1005正向 TTGCCCCGCCaagcttagcCATACTCTTTATGCCCCCGGT(序列编号68)
717反向 ACGTGGCCGCAAGCTTGCCCGCGTAATCTGGGACG(序列编号69)
1005反向 CCGGGTCGCCAAGCTTGCCCGCGTAATCTGGGAC(序列编号70)
重组风疹病毒的合成
以上述的质粒为模板，使用体外转录试剂盒(生命技术公司(LifeTechnologies))合成RNA。使用DMRIE-C(生命技术公司)将2.5μg的RNA转染于在6孔板生长的BHK细胞。对BHK细胞进行传代的同时持续培养而获得病毒。
复制子测试
风疹病毒复制子表达质粒的制备
向由风疹病毒Hiroshima株使编码结构蛋白质(C、E2和E1)的ORF缺失的基因组(亚基因组复制子)克隆而得的质粒的使结构蛋白质缺失的区域导入nano Luc基因(序列编号71)(图11)。该质粒是将非专利文献15：Sakata等2014 Journal of Virology 88：11187-11198中记载的质粒的海参荧光素酶基因部分变为nano Luc基因的质粒。另外，向P150序列的2处可导入外源基因的区域(氨基酸编号717和氨基酸编号1005)分别导入pMag-nMaghigh，获得修饰P150蛋白质(modified L)表达复制子(序列编号72和序列编号73)。
[表6]
名称 质粒序列
wt P150 序列编号71
P150-717pmag-nMaghigh 序列编号72
P150-1005pmag-nMaghigh 序列编号73
复制子测试
以上述的复制子质粒为模板，使用体外转录试剂盒(生命技术公司)合成RNA。使用DMRIE-C(生命技术公司)将1.5μg复制子RNA和1.5μg风疹病毒衣壳RNA转染于在24孔板生长的BHK细胞。转染后的细胞中，表达P150蛋白质和P90蛋白质，通过这些蛋白的聚合酶活性转录nano Luc基因，通过细胞内的翻译系统表达nano Luc。
实施例7：iPS细胞的制备
向实施例1的Magnet-L光敏性麻疹病毒的基因组中，作为转录单元整合作为重编码因子的Oct3/4、Sox2、Klf4和1-Myc，制成病毒载体。具体来说，向由包含N基因、P基因、M6489基因的第一基因组以及包含H基因和修饰L基因的第二基因组形成的双链的麻疹基因组中导入了重编码基因。第一基因组中，在N基因的上游配置Oct3、Sox2基因和1-myc基因(序列编号86)。第二基因组中，在H基因的上游配置EGFP和Klf4基因(序列编号87)(图17A)。将纤维母细胞和从人外周血获得的体细胞用适合于各细胞的培养基在37℃、5％CO2加湿气氛下培养。弃去培养基，使病毒载体以MOI＝0.1以上的条件感染细胞。感染后，将细胞用ES/iPS细胞用培养液进行培养。感染后，通过照射蓝色LED光，使各重编码因子在体细胞内表达。考察各重编码因子的表达，持续培养20天以上，诱导成iPS细胞(图17B)。
实施例8:接头的长度的探讨
向实施例1中制成的追加了EGFP转录单元的重组麻疹病毒(序列编号8)的L蛋白基因的可导入外源基因的区域导入作为光应答性基因的pMag-nMaghigh时，为了确认接头的作用，变更了接头的长度。作为所导入的光应答性基因，制成以26残基的接头结合的pMag-linker-nMaghigh(序列编号10)、以其约4倍的114残基的接头结合的pMag-4×linker-nMaghigh(序列编号80)、不介以接头直接结合的pMag-direct-nMaghigh(序列编号78)。此外，制成了使用nMag代替nMaghigh的pMag-linker-nMag(序列编号79)。将这些光应答性基因导入L蛋白质的可导入外源基因的区域，制成包含修饰L蛋白基因的麻疹病毒基因组生成用质粒。这些基因导入使用In-fusion试剂盒(克隆泰克公司)进行。导入了这些光应答性基因的质粒的序列为p(+)MV323-pMag-linker-nMaghigh(序列编号14)、p(+)MV323-pMag-4×linker-nMaghigh(序列编号81)、p(+)MV323-pMag-direct-nMaghigh(序列编号82)、p(+)MV323-pMag-linker-nMag(序列编号83)。
重组麻疹病毒的合成
使用X-tremeGENE HP(罗氏公司)向在6孔板生长的BHK/T7-9细胞转染了0.8μg的N蛋白表达质粒(pCITE-IC-N)(序列编号33)、0.6μg的P蛋白表达质粒(pCITE-IC-PΔC)(序列编号34)、1.6μg的L蛋白表达质粒(pCITE-9301B-wtL)(序列编号35)、上述的重组麻疹病毒生成用质粒(5μg)。培养2天后，将BHK/T7-9细胞叠层于Vero/hSLAM细胞，进行了病毒拯救。
光应答性的确认
使被拯救的重组麻疹病毒以MOI达到0.01的条件感染于Vero/hSLAM细胞。感染后，将感染了整合有光应答性基因的麻疹病毒的细胞分为蓝色LED(峰波长:蓝色470nm)照射组和暗处组进行培养，考察了EGFP的表达的有无(图14)。照射蓝色LED的情况下，作为光应答性基因的pMag-linker-nMaghigh(序列编号10)、pMag-4×linker-nMaghigh(序列编号80)和pMag-direct-nMaghigh(序列编号78)均同等增殖。另一方面，对于导入了pMag-linker-nMag(序列编号79)的病毒，与pMag-linker-nMaghigh相比，增殖速度差。暗处组中，对于导入了pMag-linker-nMaghigh(序列编号10)和pMag-4×linker-nMaghigh(序列编号80)的病毒，第2天起显示弱的增殖。另一方面，pMag-direct-nMaghigh(序列编号78)和pMag-linker-nMag(序列编号79)几乎未见病毒增殖。
实施例9:基于麻疹病毒的治疗活性
向5～6周龄的雌性Balb-c nu/nu小鼠以1×107细胞/小鼠从侧腹部皮下给予癌细胞株(MDA-MB-468)的悬浮液。每隔1天测定了肿瘤直径和体重。从肿瘤长径达到2mm以上时起，每隔1天以5×105IU/小鼠的浓度向肿瘤内给予重组麻疹病毒5次。使用的病毒使用作为光应答基因导入了pMag-linker-nMaghigh(序列编号10)的麻疹病毒。将小鼠分成蓝色光照射组(4只)和暗处(自然光)组(4只)饲养60天。作为对照，使用未给予病毒的暗处(自然光)组(4只)。每天观察肿瘤直径，长径达到10mm时实施安乐死。结果示于图16A和B。蓝色光照射组中，带来肿瘤尺寸的缩小，而暗处(自然光)组中，虽然可见肿瘤生长的抑制，但肿瘤尺寸未减小。可通过蓝色光照射的有无，调节麻疹病毒对癌细胞的治疗活性。
SEQUENCE LISTING
<110> 国立大学法人东京大学
<120> 光控性病毒蛋白质、其基因及包含该基因的病毒载体
<130> P19115160WP
<150> JP 2017-135579
<151> 2017-07-11
<160> 87
<170> PatentIn version 3.5
<210> 1
<211> 450
<212> DNA
<213> Artificial Sequence
<220>
<223> pMag
<400> 1
catactcttt atgcccccgg tggatatgac attatgggat atctgaggca gatcaggaac 60
cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt 120
gacctgaaac agaaagacac cccgatagtt tacgcgagtg aagccttcct ctacatgaca 180
ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc 240
atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc 300
aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga 360
cagcgatttg ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga 420
tactctatgg gattccagtg cgaaacagaa 450
<210> 2
<211> 450
<212> DNA
<213> Artificial Sequence
<220>
<223> nMaghigh
<400> 2
catactcttt atgcccccgg tggatatgac attatgggat atctggacca gatcggcaac 60
cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt 120
gacctgaaac agaaagacac cccgatagtt tacgcgagtg aagccttcct ctacatgaca 180
ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc 240
atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatccgc 300
aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga 360
cagcgatttg ttaatttcct gactatcata cctgttaggg acgaaacagg cgagtatcga 420
tactctatgg gattccagtg cgaaacagaa 450
<210> 3
<211> 675
<212> DNA
<213> Artificial Sequence
<220>
<223> DPK
<400> 3
atggtgagcg tgatcaagcc cgacatgaag atcaagctgc gcatggaggg cgccgtgaac 60
ggccacccct tcgccatcga gggcgtgggc ctgggcaagc ccttcgaggg caagcagagc 120
atggacctga aggtgaagga gggcggcccc ctgcccttcg cctacgacat cctgaccacc 180
gtgttctgct acggcaaccg cgtgttcgcc aagtaccccg agaacatcgt ggactacttc 240
aagcagagct tccccgaggg ctacagctgg gagcgcagca tgaactacga ggacggcggc 300
atctgcaacg ccaccaacga catcaccctg gacggcgact gctacatcta cgagatccgc 360
ttcgacggcg tgaacttccc cgccaacggc cccgtgatgc agaagcgcac cgtgaagtgg 420
gagcccagca ccgagaagct gtacgtgcgc gacggcgtgc tgaagggcga cgtgaacatg 480
gccctgagcc tggagggcgg cggccactac cgctgcgact tcaagaccac ctacaaggcc 540
aagaaggtgg tgcagctgcc cgactaccac ttcgtggacc accacatcga gatcaagagc 600
cacgacaagg actacagcaa cgtgaacctg cacgagcacg ccgaggccca cagcgagctg 660
ccccgccagg ccaag 675
<210> 4
<211> 675
<212> DNA
<213> Artificial Sequence
<220>
<223> DPN
<400> 4
atggtgagcg tgatcaagcc cgacatgaag atcaagctgc gcatggaggg cgccgtgaac 60
ggccacccct tcgccatcga gggcgtgggc ctgggcaagc ccttcgaggg caagcagagc 120
atggacctga aggtgaagga gggcggcccc ctgcccttcg cctacgacat cctgaccacc 180
gtgttctgct acggcaaccg cgtgttcgcc aagtaccccg agaacatcgt ggactacttc 240
aagcagagct tccccgaggg ctacagctgg gagcgcagca tgaactacga ggacggcggc 300
atctgcaacg ccaccaacga catcaccctg gacggcgact gctacatcta cgagatccgc 360
ttcgacggcg tgaacttccc cgccaacggc cccgtgatgc agaagcgcac cgtgaagtgg 420
gagcccagca ccgagaatct gtacgtgcgc gacggcgtgc tgaagggcga cgtgaacatg 480
gccctgagcc tggagggcgg cggccactac cgctgcgact tcaagaccac ctacaaggcc 540
aagaaggtgg tgcagctgcc cgactaccac ttcgtggacc accacatcga gatcaagagc 600
cacgacaagg actacagcaa cgtgaacctg cacgagcacg ccgaggccca cagcgagctg 660
ccccgccagg ccaag 675
<210> 5
<211> 1833
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<220>
<223> Cry2
<400> 5
aagatggaca aaaagactat agtttggttt agaagagacc taaggattga ggataatcct 60
gcattagcag cagctgctca cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa 120
gaagaaggac agttttatcc tggaagagct tcaagatggt ggatgaaaca atcacttgct 180
cacttatctc aatccttgaa ggctcttgga tctgacctca ctttaatcaa aacccacaac 240
acgatttcag cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac 300
cacctctatg atcctgtttc gttagttcgg gaccataccg taaaggagaa gctggtggaa 360
cgtgggatct ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg ggagatatac 420
tgcgaaaagg gcaaaccttt tacgagtttc aattcttact ggaagaaatg cttagatatg 480
tcgattgaat ccgttatgct tcctcctcct tggcggttga tgccaataac tgcagcggct 540
gaagcgattt gggcgtgttc gattgaagaa ctagggctgg agaatgaggc cgagaaaccg 600
agcaatgcgt tgttaactag agcttggtct ccaggatgga gcaatgctga taagttacta 660
aatgagttca tcgagaagca gttgatagat tatgcaaaga acagcaagaa agttgttggg 720
aattctactt cactactttc tccgtatctc catttcgggg aaataagcgt cagacacgtt 780
ttccagtgtg cccggatgaa acaaattata tgggcaagag ataagaacag tgaaggagaa 840
gaaagtgcag atctttttct taggggaatc ggtttaagag agtattctcg gtatatatgt 900
ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg 960
gatgctgatg ttgataagtt caaggcctgg agacaaggca ggaccggtta tccgttggtg 1020
gatgccggaa tgagagagct ttgggctacc ggatggatgc ataacagaat aagagtgatt 1080
gtttcaagct ttgctgtgaa gtttcttctc cttccatgga aatggggaat gaagtatttc 1140
tgggatacac ttttggatgc tgatttggaa tgtgacatcc ttggctggca gtatatctct 1200
gggagtatcc ccgatggcca cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc 1260
aaatatgacc cagaaggtga gtacataagg caatggcttc ccgagcttgc gagattgcca 1320
actgaatgga tccatcatcc atgggacgct cctttaaccg tactcaaagc ttctggtgtg 1380
gaactcggaa caaactatgc gaaacccatt gtagacatcg acacagctcg tgagctacta 1440
gctaaagcta tttcaagaac ccgtgaagca cagatcatga tcggagcagc acctgatgag 1500
attgtagcag atagcttcga ggccttaggg gctaatacca ttaaagaacc tggtctttgc 1560
ccatctgtgt cttctaatga ccaacaagta ccttcggctg ttcgttacaa cgggtcagcg 1620
gcagtgaaac ctgaggaaga agaagagaga gacatgaaga aatctagggg attcgatgaa 1680
agggagttgt tttcgactgc tgaatcttct tcttcttcga gtgtgttttt cgtttcgcag 1740
tcttgctcgt tggcatcaga agggaagaat ctggaaggta ttcaagattc atctgatcag 1800
attactacaa gtttgggaaa aaatggttgc aaa 1833
<210> 6
<211> 510
<212> DNA
<213> Artificial Sequence
<220>
<223> CIB1N
<400> 6
atgaatggag ctataggagg tgaccttttg ctcaattttc ctgacatgtc ggtcctagag 60
cgccaaaggg ctcacctcaa gtacctcaat cccacctttg attctcctct cgccggcttc 120
tttgccgatt cttcaatgat taccggcggc gagatggaca gctatctttc gactgccggt 180
ttgaatcttc cgatgatgta cggtgagacg acggtggaag gtgattcaag actctcaatt 240
tcgccggaaa cgacgcttgg gactggaaat ttcaaggcag cgaagtttga tacagagact 300
aaggattgta atgaggcggc gaagaagatg acgatgaaca gagatgacct agtagaagaa 360
ggagaagaag agaagtcgaa aataacagag caaaacaatg ggagcacaaa aagcatcaag 420
aagatgaaac acaaagccaa gaaagaagag aacaatttct ctaatgattc atctaaagtg 480
acgaaggaat tggagaaaac ggattatatt 510
<210> 7
<211> 450
<212> DNA
<213> Artificial Sequence
<220>
<223> nMag
<400> 7
catactcttt atgcccccgg tggatatgac attatgggat atctggacca gatcggcaac 60
cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt 120
gacctgaaac agaaagacac cccgatagtt tacgcgagtg aagccttcct ctacatgaca 180
ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc 240
atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc 300
aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga 360
cagcgatttg ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga 420
tactctatgg gattccagtg cgaaacagaa 450
<210> 8
<211> 16752
<212> DNA
<213> Artificial Sequence
<220>
<223> EGFP-Measles virus基因组
<400> 8
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
acgagatggc cacacttttg aggagcttag cattgttcaa aagaaacaag gacaaaccac 1020
ccattacatc aggatccggt ggagccatca gaggaatcaa acacattatt atagtaccaa 1080
ttcctggaga ttcctcaatt accactcgat ccagactact ggaccggttg gtcaggttaa 1140
ttggaaaccc ggatgtgagc gggcccaaac taacaggggc actaataggt atattatcct 1200
tatttgtgga gtctccaggt caattgattc agaggatcac cgatgaccct gacgttagca 1260
tcaggctgtt agaggttgtt cagagtgacc agtcacaatc tggccttacc ttcgcatcaa 1320
gaggtaccaa catggaggat gaggcggacc aatacttttc acatgatgat ccaagcagta 1380
gtgatcaatc caggtccgga tggttcgaga acaaggaaat ctcagatatt gaagtgcaag 1440
accctgaggg attcaacatg attctgggta ccattctagc ccagatctgg gtcttgctcg 1500
caaaggcggt tacggcccca gacacggcag ctgattcgga gctaagaagg tggataaagt 1560
acacccaaca aagaagggta gttggtgaat ttagattgga gagaaaatgg ttggatgtgg 1620
tgaggaacag gattgccgag gacctctctt tacgccgatt catggtggct ctaatcctgg 1680
atatcaagag gacacccggg aacaaaccta ggattgctga aatgatatgt gacattgata 1740
catatatcgt agaggcagga ttagccagtt ttatcctgac tattaagttt gggatagaaa 1800
ctatgtatcc tgctcttgga ctgcatgaat ttgctggtga gttatccaca cttgagtcct 1860
tgatgaatct ttaccagcaa atgggagaaa ctgcacccta catggtaatc ctagagaact 1920
caattcagaa caagttcagt gcaggatcat accctctgct ctggagctat gccatgggag 1980
taggagtgga acttgaaaac tccatgggag gtttgaactt tggtcgatct tactttgatc 2040
cagcatattt tagattaggg caagagatgg tgaggaggtc agctggaaag gtcagttcca 2100
cattggcatc cgaactcggt atcactgccg aggatgcaag gcttgtttca gagattgcaa 2160
tgcatactac tgaggacagg atcagtagag cggtcggacc cagacaagcc caagtgtcat 2220
ttctacacgg tgatcaaagt gagaatgagc taccaggatt ggggggcaag gaagatagga 2280
gggtcaaaca gggtcgggga gaagccaggg agagctacag agaaaccggg tccagcagag 2340
caagtgatgc gagagctgcc catcctccaa ccagcatgcc cctagacatt gacactgcat 2400
cggagtcagg ccaagatccg caggacagtc gaaggtcagc tgacgccctg ctcaggctgc 2460
aagccatggc aggaatcttg gaagaacaag gctcagacac ggacacccct agggtataca 2520
atgacagaga tcttctagac taggtgcgag aggccgagga ccagaacaac atccgcctac 2580
cctccatcat tgttataaaa aacttaggaa ccaggtccac acagccgcca gccaaccaac 2640
catccactcc cacgactgga gccgatggca gaagagcagg cacgccatgt caaaaacgga 2700
ctggaatgca tccgggctct caaggccgag cccatcggct cactggccgt cgaggaagcc 2760
atggcagcat ggtcagaaat atcagacaac ccaggacagg accgagccac ctgcaaggaa 2820
gaggaggcag gcagttcggg tctcagcaaa ccatgcctct cagcaattgg atcaactgaa 2880
ggcggtgcac ctcgcatccg cggtcaggga tctggagaaa gcgatgacga cgctgaaact 2940
ttgggaatcc cctcaagaaa tctccaggca tcaagcactg ggttacagtg ttatcatgtt 3000
tatgatcaca gcggtgaagc ggttaaggga atccaagatg ctgactctat catggttcaa 3060
tcaggccttg atggtgatag caccctctca ggaggagacg atgaatctga aaacagcgat 3120
gtggatattg gcgaacctga taccgaggga tatgctatca ctgaccgggg atctgctccc 3180
atctctatgg ggttcagggc ttctgatgtt gaaactgcag aaggagggga gatccacgag 3240
ctcctgaaac tccaatccag aggcaacaac tttccgaagc ttgggaaaac tctcaatgtt 3300
cctccgcccc cgaaccccag tagggccagc acttccgaga cacccattaa aaagggcaca 3360
gacgcgagat tggcctcatt tggaacggag atcgcgtctt tattgacagg tggtgcaacc 3420
caatgtgctc gaaagtcacc ctcggaacca tcagggccag gtgcacctgc ggggaatgtc 3480
cccgagtgtg tgagcaatgc cgcactgata caggagtgga cacccgaatc tggtaccaca 3540
atctccccga gatcccagaa taatgaagaa gggggagact attatgatga tgagctgttc 3600
tccgatgtcc aagacatcaa aacagccttg gccaaaatac acgaggataa tcagaagata 3660
atctccaagc tagaatcatt gctgttattg aagggagaag ttgagtcaat taagaagcag 3720
atcaacaggc aaaatatcag catatccacc ctggaaggac acctctcaag catcatgatt 3780
gccattcctg gacttgggaa ggatcccaac gaccccactg cagatgtcga actcaatccc 3840
gacctgaaac ccatcatagg cagagattca ggccgagcac tggccgaagt tctcaagaag 3900
cccgttgcca gccgacaact ccagggaatg actaatggac ggaccagttc cagaggacag 3960
ctgctgaagg aatttcaact aaagccgatc gggaaaaagg tgagctcagc cgtcgggttt 4020
gttcctgaca ccggccctgc atcacgcagt gtaatccgct ccattataaa atccagccgg 4080
ctagaggagg atcggaagcg ttacctgatg actctccttg atgatatcaa aggagccaac 4140
gatcttgcca agttccacca gatgctgatg aagataataa tgaagtagct acagctcaac 4200
ttacctgcca accccatgcc agtcgaccta attagtacaa cctaaatcca ttataaaaaa 4260
cttaggagca aagtgattgc ctcctaagtt ccacaatgac agagatctac gatttcgaca 4320
agtcggcatg ggacatcaaa gggtcgatcg ctccgataca acctaccacc tacagtgatg 4380
gcaggctggt gccccaggtc agagtcatag atcctggtct aggtgatagg aaggatgaat 4440
gctttatgta catgtttctg ctgggggttg ttgaggacag cgatccccta gggcctccaa 4500
tcgggcgagc attcgggtcc ctgcccttag gtgttggtag atccacagca aaacccgagg 4560
aactcctcaa agaggccact gagcttgaca tagttgttag acgtacagca gggctcaatg 4620
aaaaactggt gttctacaac aacaccccac taaccctcct cacaccttgg agaaaggtcc 4680
taacaacagg gagtgtcttc aatgcaaacc aagtgtgcaa tgcggttaat ctaataccgc 4740
tggacacccc gcagaggttc cgtgttgttt atatgagcat cacccgtctt tcggataacg 4800
ggtattacac cgttcccaga agaatgctgg aattcagatc ggtcaatgca gtggccttca 4860
acctgctagt gacccttagg attgacaagg cgattggccc tgggaagatc atcgacaatg 4920
cagagcaact tcctgaggca acatttatgg tccacatcgg gaacttcagg agaaagaaga 4980
gtgaagtcta ctctgccgat tattgcaaaa tgaaaatcga aaagatgggc ctggtttttg 5040
cacttggtgg gatagggggc accagtcttc acattagaag cacaggcaaa atgagcaaga 5100
ctctccatgc acaactcggg ttcaagaaga ccttatgtta cccactgatg gatatcaatg 5160
aagaccttaa tcggttactc tggaggagca gatgcaagat agtaagaatc caggcagttt 5220
tgcagccatc agttcctcaa gaattccgca tttacgacga cgtgatcata aatgatgacc 5280
aaggactatt caaagttctg tagaccgcag tgcccagcaa tacccgaaaa cgacccccct 5340
cataatgaca gccagaaggc ccggacaaaa aagccccctc cagaagactc cacggaccaa 5400
gcgagaggcc agccagcagc cgacagcaag tgtggacacc aggcggccca agcacagaac 5460
agccccgaca caaggccacc accagccatc ccaatctgcg tcctcctcgt gggacccccg 5520
aggaccaacc ccgaaggtcg ctccgaacac agaccaccaa ccgcatcccc acagctcccg 5580
ggaaaggaac ccccagcaac tggaaggccc ctccccccct cccccaacgc aagaacccca 5640
caaccgaacc gcacaagcga ccgaggtgac ccaaccgcag gcatccgact ccttagacag 5700
atcctctccc cccggcatac taaacaaaac ttagggccaa ggaacacaca cactcgacag 5760
aacccagacc ccggcccgcg gcaccgcgcc cccacccccc gaaaaccaga gggagccccc 5820
aaccaaaccc gccggccccc ccggtgccca caggtaggca caccaacccc cgaccagacc 5880
cagcacccag ccaccgacaa tccaagacgg ggggcccccc ccaaaaaaag gcccccaggg 5940
gccgacagcc agcatcgcga ggaagcacac ccaccccaca cacgaccacg gcaaccgaac 6000
cagagtccag accaccctgg gccaccagct cccagactcg gccatcaccc cgcaaaaagg 6060
aaaggccaca acccgcgcac cccagccccg atccggcggg cagccactca acccgaacca 6120
gcacccaaga gcgatccctg ggggaccccc aaaccgcaaa agacatcagt atcccacagc 6180
ctctccaagt cccccggtct cctcctcttc tcgaagggac caaaagatca atccaccaca 6240
tccgacgaca ctcaattccc cacccccaaa ggagacaccg ggaatcccag aatcaagact 6300
catccagtgt ccatcatggg tctcaaggtg aacgtctctg ccatattcat ggcagtactg 6360
ttaactctcc aaacacccac cggtcaaatc cattggggca atctctctaa gataggggtg 6420
gtagggatag gaagtgcaag ctacaaagtt atgactcgtt ccagccatca atcattggtc 6480
ataaaattaa tgcccaatat aactctcctc aataactgca cgagggtaga gatcgcagaa 6540
tacaggagac tactgagaac agttttggaa ccaattagag atgcacttaa tgcaatgacc 6600
cagaatataa gaccggttca gagtgtagct tcaagtagga gacacaagag atttgcggga 6660
gttgtcctgg caggtgcggc cctaggcgtt gccacagctg ctcagataac agccggcatt 6720
gcacttcacc agtccatgct gaactctcaa gccatcgaca atctgagagc aagcctggaa 6780
actactaatc aggcaattga ggcaatcaga caagcagggc aggagatgat attggctgtt 6840
cagggtgtcc aagactacat caataatgag ctgataccgt ctatgaacca actatcttgt 6900
gatttaatcg gccagaagct agggctcaaa ttgctcagat actatacaga aatcctgtca 6960
ttatttggcc ccagcttacg ggaccccata tctgcggaga tatccatcca ggctttgagc 7020
tatgcgcttg gaggagatat caataaggta ttagaaaagc tcggatacag tggaggtgat 7080
ttactgggca tcttagagag cagaggaata aaggcccgga taactcacgt cgacacagag 7140
tcctacttca ttgtactcag tatagcctat ccgacgctgt ccgagattaa gggggtgatt 7200
gtccaccggc tagagggggt ctcgtacaat ataggctctc aagagtggta taccactgtg 7260
cccaagtatg ttgcaaccca agggtacctt atctcgaatt ttgatgagtc atcgtgtact 7320
ttcatgccag aggggactgt gtgcagccaa aatgccttgt acccgatgag tcctctgctc 7380
caagaatgcc tccgggggtc caccaagtcc tgtgctcgta cactcgtatc cgggtctttt 7440
gggaaccggt tcattttatc acaagggaac ctaatagcca attgtgcatc aatcctctgc 7500
aagtgttaca caacaggaac gatcattaat caagaccctg acaagatcct aacatacatt 7560
gctgccgatc actgcccggt ggtcgaggtg aacggtgtga ccatccaagt cgggagcagg 7620
aggtatccgg acgcggtgta cctgcacaga attgacctcg gtcctcccat atcattggag 7680
aggttggacg tagggacaaa tctggggaat gcaattgcta agttggagga tgccaaggaa 7740
ttgttggagt catcggacca gatattgagg agtatgaaag gtttatcgag cactagcata 7800
gtttacatcc tgattgcagt gtgtcttgga gggttgatag ggatccccgc tttaatatgt 7860
tgctgcaggg ggcgctgtaa caaaaaggga gaacaagttg gtatgtcaag accaggccta 7920
aagcctgatc ttacagggac atcaaaatcc tatgtaaggt cgctctgatc ctctacaact 7980
cttgaaacac agatttccca caagtctcct cttcgtcatc aagcaaccac cgcatccagc 8040
atcaagccca cctgaaattg tctccggctt ccctctggcc gaacgatatc ggtagttaat 8100
taaaacttag ggtgcaagat catccacaat gtcaccacaa cgagaccgaa taaatgcctt 8160
ctacaaagac aacccacatc ctaagggaag taggatagtt attaacagag aacatcttat 8220
gattgataga ccttatgttt tgctggctgt tctattcgtc atgtttctga gcttgatcgg 8280
gttgctagcc attgcaggca ttagactcca tcgtgcagcc atctacaccg cagagatcca 8340
taagagcctc agcaccaatc tagatgtaac taactcgatc gagcatcagg tcaaggacgt 8400
gctgacacca ctcttcaaga tcattggtga tgaagtgggc ctgaggacac ctcagagatt 8460
cactgaccta gtgaaattca tctctgacaa aattaaattc cttaatccgg atagggagta 8520
cgacttcaga gatctcactt ggtgtatcaa cccgccagag agaatcaaat tggattatga 8580
tcaatactgt gcagatgtgg ctgctgaaga actcatgaat gcattggtga actcaactct 8640
actggaggcc agggcaacca atcagttcct agctgtctca aagggaaact gctcagggcc 8700
cactacaatc agaggtcaat tctcaaacat gtcgctgtcc ctgttggact tgtatttaag 8760
tcgaggttac aatgtgtcat ctatagtcac tatgacatcc cagggaatgt acgggggaac 8820
ttacctagtg ggaaagccta atctgagcag taaagggtca gagttgtcac aactgagcat 8880
gcaccgagtg tttgaagtag gggttatcag aaatccgggt ttgggggctc cggtgttcca 8940
tatgacaaac tattttgagc aaccagtcag taatgatttc agcaactgca tggtggcttt 9000
gggggagctt aaattcgcag ccctctgtca cagggaagat tctatcacaa ttccctatca 9060
ggggtcaggg aaaggtgtca gcttccagct cgtcaagcta ggtgtctgga aatccccaac 9120
cgacatgcga tcctgggtcc ccctatcaac ggatgatcca gtgatagata ggctttatct 9180
ctcatctcac agaggtgtta tcgctgacaa tcaagcaaaa tgggctgtcc cgacaacacg 9240
gacagatgac aagttgcgaa tggagacatg cttccagcag gcgtgtaagg gtaaaaacca 9300
agcactctgc gagaatcccg agtgggcacc attgaaggat aacaggattc cttcatacgg 9360
ggtcttgtct gttaatctga gtctgacagt tgagcttaaa atcaaaattg cttcaggatt 9420
cgggccattg atcacacacg gttcagggat ggacctatac aaaaccaacc acaacaatgt 9480
gtattggctg actatcccgc caatgaagaa cctagcctta ggtgtaatca acacattgga 9540
gtggataccg agattcaagg ttagtcccaa cctcttcact gttccaatca aggaagcagg 9600
cgaggactgc catgccccaa catacctacc tgcggaggtg gatggtgatg tcaaactcag 9660
ttccaatctg gtaattctac ctggtcagga tctccaatat gttttggcaa cctacgatac 9720
ttccagggtt gaacatgctg tggtttatta tgtttacagc ccaagccgct cattttctta 9780
cttttatcct tttaggttgc ctataaaggg ggtcccaatc gaattacaag tggaatgctt 9840
cacatgggac aaaaaactct ggtgccgtca cttctgtgtg cttgcggact cagaatctgg 9900
tggacatatc actcactctg ggatggtggg catgggagtc agctgcacag tcactcggga 9960
agatggaacc aatcgcagat agggctgcca gtgaaccgat cacatgatgt cacccagaca 10020
tcaggcatac ccactagtgt gaaatagaca tcagaattaa gaaaaacgta gggtccaagt 10080
ggtttcccgt tatggactcg ctatctgtca accagatctt ataccctgaa gttcacctag 10140
atagcccgat agttaccaat aagatagtag ctatcctgga gtatgctcga gtccctcacg 10200
cttacagcct ggaggaccct acactgtgtc agaacatcaa gcaccgccta aaaaacggat 10260
tctccaacca aatgattata aacaatgtgg aagttgggaa tgtcatcaag tccaagctta 10320
ggagttatcc ggcccactct catattccat atccaaattg taatcaggat ttatttaaca 10380
tagaagacaa agagtcaaca aggaagatcc gtgagctcct aaaaaaggga aattcgctgt 10440
actccaaagt cagtgataag gttttccaat gcctgaggga cactaactca cggcttggcc 10500
taggctccga attgagggag gacatcaagg agaaaattat taacttggga gtttacatgc 10560
acagctccca atggtttgag ccctttctgt tttggtttac agtcaagact gagatgaggt 10620
cagtgattaa atcacaaacc catacttgcc ataggaggag acacacacct gtattcttca 10680
ctggtagttc agttgagctg ttaatctctc gtgaccttgt tgctataatc agtaaggaat 10740
ctcaacatgt atattacctg acgtttgaac tggttttgat gtattgtgat gtcatagagg 10800
ggaggttaat gacagagacc gctatgacca ttgatgctag gtatgcagaa cttctaggaa 10860
gagtcagata catgtggaaa ctgatagatg gtttcttccc tgcactcggg aatccaactt 10920
atcaaattgt agccatgctg gagccacttt cacttgctta cctgcaactg agggatataa 10980
cagtagaact cagaggtgct ttccttaacc actgctttac tgaaatacat gatgttcttg 11040
accaaaacgg gttttctgat gaaggtactt atcatgagtt aattgaagcc ctagattaca 11100
ttttcataac tgatgacata catctgacag gggagatttt ctcatttttc agaagtttcg 11160
gccaccccag acttgaagca gtaacggctg ctgaaaatgt caggaaatac atgaatcagc 11220
ctaaagtcat tgtgtatgag actctgatga aaggtcatgc catattttgt ggaatcataa 11280
tcaacggcta tcgtgacagg cacggaggca gttggccacc cctgaccctc cccctgcatg 11340
ctgcagacac aatccggaat gctcaagctt caggtgaagg gttaacacat gagcagtgcg 11400
ttgataactg gaaatcattt gctggagtga gatttggctg ttttatgcct cttagcctgg 11460
acagtgatct gacaatgtac ctaaaggaca aggcacttgc tgctctccaa agggaatggg 11520
attcagttta cccgaaagag ttcctgcgtt acgatcctcc caagggaacc gggtcacgga 11580
ggcttgtaga tgttttcctt aatgattcga gctttgaccc atatgatatg ataatgtatg 11640
tcgtaagtgg agcctacctc catgaccctg agttcaacct gtcttacagc ctgaaagaaa 11700
aggagatcaa ggaaacaggt agacttttcg ctaaaatgac ttacaaaatg agggcatgcc 11760
aagtgatcgc tgaaaatcta atctcaaacg ggattggcaa gtattttaag gacaatggga 11820
tggccaagga tgagcacgat ttgactaagg cactccacac tctggctgtc tcaggagtcc 11880
ccaaagatct caaagaaagt cacagggggg ggccagtctt aaaaacctac tcccgaagcc 11940
cagtccacac aagtaccagg aacgttaaag cagaaaaagg gtttgtagga ttccctcatg 12000
taattcggca gaatcaagac actgatcatc cggagaatat agaaacctac gagacagtca 12060
gcgcatttat cacgactgat ctcaagaagt actgccttaa ttggagatat gagaccatca 12120
gcttatttgc acagaggcta aatgagattt acggattacc ctcatttttt cagtggctgc 12180
ataagaggct tgaaacctct gtcctctatg taagtgaccc tcattgcccc cccgaccttg 12240
acgcccatgt cccgttatgc aaagtcccca atgaccaaat cttcatcaag taccctatgg 12300
gaggtataga agggtattgt cagaagctgt ggaccatcag caccattccc tacttatacc 12360
tggctgctta tgagagcggg gtaaggattg cttcgttagt gcaaggggac aatcagacca 12420
tagccgtaac aaaaagggta cccagcacat ggccttacaa ccttaagaaa cgggaagctg 12480
ctagagtaac tagagattac tttgtaattc ttaggcaaag gctacatgac attggccatc 12540
acctcaaggc aaatgagaca attgtttcat cacatttttt tgtctattca aaaggaatat 12600
attatgatgg gctacttgtg tcccaatcac tcaagagcat cgcaagatgt gtattctggt 12660
cagagactat agttgatgaa acaagggcgg catgcagtaa tattgctaca acaatggcca 12720
aaagcattga gagaggttat gaccgttatc ttgcatattc cctgaacgtc ctaaaagtga 12780
tacagcaaat tttgatctcc cttggcttca caatcaattc aaccatgacc cgggatgtag 12840
tcatacccct cctcacaaac aacgatctct taataaggat ggcactgttg cccgctccta 12900
ttgggggtat gaattatctg aatatgagca ggctgtttgt cagaaacatc ggtgatccag 12960
taacatcatc aattgctgat ctcaagagaa tgattctcgc atcactaatg cctgaagaga 13020
ccctccatca agtaatgaca caacaaccgg gggactcttc attcctagac tgggctagcg 13080
acccttactc agcaaatctt gtatgcgtcc agagcatcac tagactcctc aagaacataa 13140
ctgcaaggtt tgtcctgatc cacagtccaa acccaatgtt aaaagggtta ttccatgatg 13200
acagcaaaga agaggacgag agactggcgg cattcctcat ggacaggcat attatagtac 13260
ctagggcagc tcatgaaatc ctggatcata gtgtcacagg ggcaagagag tctattgcag 13320
gcatgctaga taccacaaaa ggcctgattc gagccagcat gaggaagggg gggttaacct 13380
ctcgagtgat aaccagattg tccaattatg actatgaaca atttagagca gggatggtgc 13440
tattgacagg aagaaagaga aatgtcctca ttgacaaaga gtcatgttca gtgcagctgg 13500
ctagagccct aagaagccat atgtgggcaa gactagctcg aggacggcct atttacggcc 13560
ttgaggtccc tgatgtacta gaatctatgc gaggccacct tattcggcgt catgagacat 13620
gtgtcatctg cgagtgtgga tcagtcaact acggatggtt ttttgtcccc tcgggttgcc 13680
aactggatga tattgacaag gaaacatcat ccttgagagt cccatatatt ggttctacca 13740
ctgatgagag aacagacatg aagctcgcct tcgtaagagc cccaagtaga tccttgcgat 13800
ctgccgttag aatagcaaca gtgtactcat gggcttacgg tgatgatgat agctcttgga 13860
acgaagcctg gttgttggca aggcaaaggg ccaatgtgag cctggaggag ctaagggtga 13920
tcactcccat ctcgacttcg actaatttag cgcataggtt gagggatcgt agcactcaag 13980
tgaaatactc aggtacatcc cttgtccgag tggcaaggta taccacaatc tccaacgaca 14040
atctctcatt tgtcatatca gataagaagg ttgatactaa ctttatatac caacaaggaa 14100
tgcttctagg gttgggtgtt ttagaaacat tgtttcgact cgagaaagat actggatcat 14160
ctaacacggt attacatctt cacgtcgaaa cagattgttg cgtgatcccg atgatagatc 14220
atcccaggat acccagctcc cgcaagctag agctgagggc agagctatgt accaacccat 14280
tgatatatga taatgcacct ttaattgaca gagatgcaac aaggctatac acccagagcc 14340
ataggaggca ccttgtggaa tttgttacat ggtccacacc ccaactatat cacattctag 14400
ctaagtccac agcactatct atgattgacc tggtaacaaa atttgagaag gaccatatga 14460
atgaaatttc agctctcata ggggatgacg atatcaatag tttcataact gagtttctgc 14520
ttatagagcc aagattattc accatctact tgggccagtg tgcagccatc aattgggcat 14580
ttgatgtaca ttatcataga ccatcaggga aatatcagat gggtgagctg ttgtcttcgt 14640
tcctttctag aatgagcaaa ggagtgttta aggtgcttgt caatgctcta agccacccaa 14700
agatctacaa gaaattctgg cattgtggta ttatagagcc tatccatggt ccttcacttg 14760
atgctcaaaa cttgcacaca actgtgtgca acatggttta cacatgctat atgacctacc 14820
tcgacctgtt gttgaatgaa gagttagaag agttcacatt tcttttgtgt gaaagcgatg 14880
aggatgtagt accggacaga ttcgacaaca tccaggcaaa acacttgtgt gttctggcag 14940
atttgtactg tcaaccaggg acctgcccac cgattcgagg tctaaggccg gtagagaaat 15000
gtgcagttct aaccgatcat atcaaggcag aggctaggtt atctccagca ggatcttcgt 15060
ggaacataaa tccaattatt gtagaccatt actcatgctc tctgacttat ctccgtcgag 15120
gatctatcaa acagataaga ttgagagttg atccaggatt catttttgac gccctcgctg 15180
aggtaaatgt cagtcagcca aaggtcggca gcaacaacat ctcaaatatg agcatcaagg 15240
atttcagacc tccacacgat gatgttgcaa aattgctcaa agatatcaac acaagcaagc 15300
acaatcttcc catttcaggg ggtagtctcg ccaattatga aatccatgct ttccgcagaa 15360
tcgggttaaa ctcatctgct tgctacaaag ctgttgagat atcaacatta attaggagat 15420
gccttgagcc aggggaagac ggcttgttct tgggtgaggg gtcgggttct atgttgatca 15480
cttataagga gatactaaaa ctaaacaagt gcttctataa tagtggggtt tccgccaatt 15540
ctagatctgg tcaaagggaa ttagcaccct atccctccga agttggcctt gtcgaacaca 15600
gaatgggagt aggtaatatt gtcaaggtgc tctttaacgg gaggcccgaa gtcacgtggg 15660
taggcagtat agattgcttc aatttcatag tcagtaatat ccctacctct agtgtggggt 15720
ttatccattc agatatagag accttaccta acaaagatac tatagagaag ctagaggaat 15780
tggcagccat cttatcgatg gctctactcc ttggcaaaat aggatcaata ctggtgatta 15840
agcttatgcc tttcagcggg gattttgttc agggatttat aagctatgta gggtctcatt 15900
atagagaagt gaaccttgtc taccctaggt acagcaactt catatctact gaatcttatt 15960
tagttatgac agatctcaaa gctaaccggc taatgaatcc tgaaaagatc aagcagcaga 16020
taattgaatc atctgtacgg acttcacctg gacttatagg tcacatccta tccattaagc 16080
aactaagctg catacaagca attgtgggag gcgcggttag tagaggtgat atcaacccta 16140
ttctgaaaaa acttacacct atagagcagg tgctgatcag ttgcgggttg gcaattaacg 16200
gacctaaact gtgcaaagaa ttaatccacc atgatgttgc ctcagggcaa gatggattgc 16260
ttaactctat actcatcctc tacagggagt tggcaagatt caaagacaac caaagaagtc 16320
aacaagggat gttccacgct taccccgtat tggtaagtag taggcaacga gaacttgtat 16380
ctaggatcac tcgcaaattt tgggggcata ttcttcttta ctccgggaac agaaagttga 16440
taaatcggtt tatccagaat ctcaagtccg gttatctagt actagactta caccagaata 16500
tcttcgttaa gaatctatcc aagtcagaga aacagattat tatgacgggg ggtttaaaac 16560
gtgagtgggt ttttaaggta acagtcaagg agaccaaaga atggtacaag ttagtcggat 16620
acagcgctct gattaaggat taattggttg aactccggaa ccctaatcct gccctaggta 16680
gttaggcatt atttgcaata tattaaagaa aactttgaaa atacgaagtt tctattccca 16740
gctttgtctg gt 16752
<210> 9
<211> 1428
<212> DNA
<213> Artificial Sequence
<220>
<223> DP
<400> 9
atggtgagcg tgatcaagcc cgacatgaag atcaagctgc gcatggaggg cgccgtgaac 60
ggccacccct tcgccatcga gggcgtgggc ctgggcaagc ccttcgaggg caagcagagc 120
atggacctga aggtgaagga gggcggcccc ctgcccttcg cctacgacat cctgaccacc 180
gtgttctgct acggcaaccg cgtgttcgcc aagtaccccg agaacatcgt ggactacttc 240
aagcagagct tccccgaggg ctacagctgg gagcgcagca tgaactacga ggacggcggc 300
atctgcaacg ccaccaacga catcaccctg gacggcgact gctacatcta cgagatccgc 360
ttcgacggcg tgaacttccc cgccaacggc cccgtgatgc agaagcgcac cgtgaagtgg 420
gagcccagca ccgagaagct gtacgtgcgc gacggcgtgc tgaagggcga cgtgaacatg 480
gccctgagcc tggagggcgg cggccactac cgctgcgact tcaagaccac ctacaaggcc 540
aagaaggtgg tgcagctgcc cgactaccac ttcgtggacc accacatcga gatcaagagc 600
cacgacaagg actacagcaa cgtgaacctg cacgagcacg ccgaggccca cagcgagctg 660
ccccgccagg ccaagaccgg taattccgct gacggcggcg gaggatcggg tggtagtggt 720
ggttcaggag gaggatcgac ccaaggagga tccatggtga gcgtgatcaa gcccgacatg 780
aagatcaagc tgcgcatgga gggcgccgtg aacggccacc ccttcgccat cgagggcgtg 840
ggcctgggca agcccttcga gggcaagcag agcatggacc tgaaggtgaa ggagggcggc 900
cccctgccct tcgcctacga catcctgacc accgtgttct gctacggcaa ccgcgtgttc 960
gccaagtacc ccgagaacat cgtggactac ttcaagcaga gcttccccga gggctacagc 1020
tgggagcgca gcatgaacta cgaggacggc ggcatctgca acgccaccaa cgacatcacc 1080
ctggacggcg actgctacat ctacgagatc cgcttcgacg gcgtgaactt ccccgccaac 1140
ggccccgtga tgcagaagcg caccgtgaag tgggagccca gcaccgagaa tctgtacgtg 1200
cgcgacggcg tgctgaaggg cgacgtgaac atggccctga gcctggaggg cggcggccac 1260
taccgctgcg acttcaagac cacctacaag gccaagaagg tggtgcagct gcccgactac 1320
cacttcgtgg accaccacat cgagatcaag agccacgaca aggactacag caacgtgaac 1380
ctgcacgagc acgccgaggc ccacagcgag ctgccccgcc aggccaag 1428
<210> 10
<211> 981
<212> DNA
<213> Artificial Sequence
<220>
<223> pMag-linker-nMaghigh
<400> 10
catactcttt atgcccccgg tggatatgac attatgggat atctgaggca gatcaggaac 60
cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt 120
gacctgaaac agaaagacac cccgatagtt tacgcgagtg aagccttcct ctacatgaca 180
ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc 240
atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc 300
aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga 360
cagcgatttg ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga 420
tactctatgg gattccagtg cgaaacagaa accggtaatt ccgctgacgg cggcggagga 480
tcgggtggta gtggtggttc aggaggagga tcgacccaag gaggatccat gcatactctt 540
tatgcccccg gtggatatga cattatggga tatctggacc agatcggcaa ccggccaaac 600
ccgcaggtgg aactgggccc cgtggataca tcctgcgcct tgattctttg tgacctgaaa 660
cagaaagaca ccccgatagt ttacgcgagt gaagccttcc tctacatgac aggttacagc 720
aacgcagagg tgctgggccg gaattgccgg tttctgcaaa gccctgacgg catggtgaag 780
cccaagagca cccggaagta cgtggatagt aacacaatca atactatccg caaggcaatc 840
gacaggaatg ccgaggtgca ggttgaagta gtcaatttta aaaagaatgg acagcgattt 900
gttaatttcc tgactatcat acctgttagg gacgaaacag gcgagtatcg atactctatg 960
ggattccagt gcgaaacaga a 981
<210> 11
<211> 2079
<212> DNA
<213> Artificial Sequence
<220>
<223> Cry2-linker-CIB1N
<400> 11
aagatggaca aaaagactat agtttggttt agaagagacc taaggattga ggataatcct 60
gcattagcag cagctgctca cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa 120
gaagaaggac agttttatcc tggaagagct tcaagatggt ggatgaaaca atcacttgct 180
cacttatctc aatccttgaa ggctcttgga tctgacctca ctttaatcaa aacccacaac 240
acgatttcag cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac 300
cacctctatg atcctgtttc gttagttcgg gaccataccg taaaggagaa gctggtggaa 360
cgtgggatct ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg ggagatatac 420
tgcgaaaagg gcaaaccttt tacgagtttc aattcttact ggaagaaatg cttagatatg 480
tcgattgaat ccgttatgct tcctcctcct tggcggttga tgccaataac tgcagcggct 540
gaagcgattt gggcgtgttc gattgaagaa ctagggctgg agaatgaggc cgagaaaccg 600
agcaatgcgt tgttaactag agcttggtct ccaggatgga gcaatgctga taagttacta 660
aatgagttca tcgagaagca gttgatagat tatgcaaaga acagcaagaa agttgttggg 720
aattctactt cactactttc tccgtatctc catttcgggg aaataagcgt cagacacgtt 780
ttccagtgtg cccggatgaa acaaattata tgggcaagag ataagaacag tgaaggagaa 840
gaaagtgcag atctttttct taggggaatc ggtttaagag agtattctcg gtatatatgt 900
ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg 960
gatgctgatg ttgataagtt caaggcctgg agacaaggca ggaccggtta tccgttggtg 1020
gatgccggaa tgagagagct ttgggctacc ggatggatgc ataacagaat aagagtgatt 1080
gtttcaagct ttgctgtgaa gtttcttctc cttccatgga aatggggaat gaagtatttc 1140
tgggatacac ttttggatgc tgatttggaa tgtgacatcc ttggctggca gtatatctct 1200
gggagtatcc ccgatggcca cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc 1260
aaatatgacc cagaaggtga gtacataagg caatggcttc ccgagcttgc gagattgcca 1320
actgaatgga tccatcatcc atgggacgct cctttaaccg tactcaaagc ttctggtgtg 1380
gaactcggaa caaactatgc gaaacccatt gtagacatcg acacagctcg tgagctacta 1440
gctaaagcta tttcaagaac ccgtgaagca cagatcatga tcggagcagc aaccggtaat 1500
tccgctgacg gcggcggagg atcgggtggt agtggtggtt caggaggagg atcgacccaa 1560
ggaggatcca tgaatggagc tataggaggt gaccttttgc tcaattttcc tgacatgtcg 1620
gtcctagagc gccaaagggc tcacctcaag tacctcaatc ccacctttga ttctcctctc 1680
gccggcttct ttgccgattc ttcaatgatt accggcggcg agatggacag ctatctttcg 1740
actgccggtt tgaatcttcc gatgatgtac ggtgagacga cggtggaagg tgattcaaga 1800
ctctcaattt cgccggaaac gacgcttggg actggaaatt tcaaggcagc gaagtttgat 1860
acagagacta aggattgtaa tgaggcggcg aagaagatga cgatgaacag agatgaccta 1920
gtagaagaag gagaagaaga gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa 1980
agcatcaaga agatgaaaca caaagccaag aaagaagaga acaatttctc taatgattca 2040
tctaaagtga cgaaggaatt ggagaaaacg gattatatt 2079
<210> 12
<211> 2079
<212> DNA
<213> Artificial Sequence
<220>
<223> CRY2PHR-linker-CIB1N
<400> 12
aagatggaca aaaagactat agtttggttt agaagagacc taaggattga ggataatcct 60
gcattagcag cagctgctca cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa 120
gaagaaggac agttttatcc tggaagagct tcaagatggt ggatgaaaca atcacttgct 180
cacttatctc aatccttgaa ggctcttgga tctgacctca ctttaatcaa aacccacaac 240
acgatttcag cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac 300
cacctctatg atcctgtttc gttagttcgg gaccataccg taaaggagaa gctggtggaa 360
cgtgggatct ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg ggagatatac 420
tgcgaaaagg gcaaaccttt tacgagtttc aattcttact ggaagaaatg cttagatatg 480
tcgattgaat ccgttatgct tcctcctcct tggcggttga tgccaataac tgcagcggct 540
gaagcgattt gggcgtgttc gattgaagaa ctagggctgg agaatgaggc cgagaaaccg 600
agcaatgcgt tgttaactag agcttggtct ccaggatgga gcaatgctga taagttacta 660
aatgagttca tcgagaagca gttgatagat tatgcaaaga acagcaagaa agttgttggg 720
aattctactt cactactttc tccgtatctc catttcgggg aaataagcgt cagacacgtt 780
ttccagtgtg cccggatgaa acaaattata tgggcaagag ataagaacag tgaaggagaa 840
gaaagtgcag atctttttct taggggaatc ggtttaagag agtattctcg gtatatatgt 900
ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg 960
gatgctgatg ttgataagtt caaggcctgg agacaaggca ggaccggtta tccgttggtg 1020
gatgccggaa tgagagagct ttgggctacc ggatggatgc ataacagaat aagagtgatt 1080
gtttcaagct ttgctgtgaa gtttcttctc cttccatgga aatggggaat gaagtatttc 1140
tgggatacac ttttggatgc tgatttggaa tgtgacatcc ttggctggca gtatatctct 1200
gggagtatcc ccgatggcca cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc 1260
aaatatgacc cagaaggtga gtacataagg caatggcttc ccgagcttgc gagattgcca 1320
actgaatgga tccatcatcc atgggacgct cctttaaccg tactcaaagc ttctggtgtg 1380
gaactcggaa caaactatgc gaaacccatt gtagacatcg acacagctcg tgagctacta 1440
gctaaagcta tttcaagaac ccgtgaagca cagatcatga tcggagcagc aaccggtaat 1500
tccgctgacg gcggcggagg atcgggtggt agtggtggtt caggaggagg atcgacccaa 1560
ggaggatcca tgaatggagc tataggaggt gaccttttgc tcaattttcc tgacatgtcg 1620
gtcctagagc gccaaagggc tcacctcaag tacctcaatc ccacctttga ttctcctctc 1680
gccggcttct ttgccgattc ttcaatgatt accggcggcg agatggacag ctatctttcg 1740
actgccggtt tgaatcttcc gatgatgtac ggtgagacga cggtggaagg tgattcaaga 1800
ctctcaattt cgccggaaac gacgcttggg actggaaatt tcaaggcagc gaagtttgat 1860
acagagacta aggattgtaa tgaggcggcg aagaagatga cgatgaacag agatgaccta 1920
gtagaagaag gagaagaaga gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa 1980
agcatcaaga agatgaaaca caaagccaag aaagaagaga acaatttctc taatgattca 2040
tctaaagtga cgaaggaatt ggagaaaacg gattatatt 2079
<210> 13
<211> 21146
<212> DNA
<213> Artificial Sequence
<220>
<223> p(+)MV323-DPK?DPN
<400> 13
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
acgagatggc cacacttttg aggagcttag cattgttcaa aagaaacaag gacaaaccac 1020
ccattacatc aggatccggt ggagccatca gaggaatcaa acacattatt atagtaccaa 1080
ttcctggaga ttcctcaatt accactcgat ccagactact ggaccggttg gtcaggttaa 1140
ttggaaaccc ggatgtgagc gggcccaaac taacaggggc actaataggt atattatcct 1200
tatttgtgga gtctccaggt caattgattc agaggatcac cgatgaccct gacgttagca 1260
tcaggctgtt agaggttgtt cagagtgacc agtcacaatc tggccttacc ttcgcatcaa 1320
gaggtaccaa catggaggat gaggcggacc aatacttttc acatgatgat ccaagcagta 1380
gtgatcaatc caggtccgga tggttcgaga acaaggaaat ctcagatatt gaagtgcaag 1440
accctgaggg attcaacatg attctgggta ccattctagc ccagatctgg gtcttgctcg 1500
caaaggcggt tacggcccca gacacggcag ctgattcgga gctaagaagg tggataaagt 1560
acacccaaca aagaagggta gttggtgaat ttagattgga gagaaaatgg ttggatgtgg 1620
tgaggaacag gattgccgag gacctctctt tacgccgatt catggtggct ctaatcctgg 1680
atatcaagag gacacccggg aacaaaccta ggattgctga aatgatatgt gacattgata 1740
catatatcgt agaggcagga ttagccagtt ttatcctgac tattaagttt gggatagaaa 1800
ctatgtatcc tgctcttgga ctgcatgaat ttgctggtga gttatccaca cttgagtcct 1860
tgatgaatct ttaccagcaa atgggagaaa ctgcacccta catggtaatc ctagagaact 1920
caattcagaa caagttcagt gcaggatcat accctctgct ctggagctat gccatgggag 1980
taggagtgga acttgaaaac tccatgggag gtttgaactt tggtcgatct tactttgatc 2040
cagcatattt tagattaggg caagagatgg tgaggaggtc agctggaaag gtcagttcca 2100
cattggcatc cgaactcggt atcactgccg aggatgcaag gcttgtttca gagattgcaa 2160
tgcatactac tgaggacagg atcagtagag cggtcggacc cagacaagcc caagtgtcat 2220
ttctacacgg tgatcaaagt gagaatgagc taccaggatt ggggggcaag gaagatagga 2280
gggtcaaaca gggtcgggga gaagccaggg agagctacag agaaaccggg tccagcagag 2340
caagtgatgc gagagctgcc catcctccaa ccagcatgcc cctagacatt gacactgcat 2400
cggagtcagg ccaagatccg caggacagtc gaaggtcagc tgacgccctg ctcaggctgc 2460
aagccatggc aggaatcttg gaagaacaag gctcagacac ggacacccct agggtataca 2520
atgacagaga tcttctagac taggtgcgag aggccgagga ccagaacaac atccgcctac 2580
cctccatcat tgttataaaa aacttaggaa ccaggtccac acagccgcca gccaaccaac 2640
catccactcc cacgactgga gccgatggca gaagagcagg cacgccatgt caaaaacgga 2700
ctggaatgca tccgggctct caaggccgag cccatcggct cactggccgt cgaggaagcc 2760
atggcagcat ggtcagaaat atcagacaac ccaggacagg accgagccac ctgcaaggaa 2820
gaggaggcag gcagttcggg tctcagcaaa ccatgcctct cagcaattgg atcaactgaa 2880
ggcggtgcac ctcgcatccg cggtcaggga tctggagaaa gcgatgacga cgctgaaact 2940
ttgggaatcc cctcaagaaa tctccaggca tcaagcactg ggttacagtg ttatcatgtt 3000
tatgatcaca gcggtgaagc ggttaaggga atccaagatg ctgactctat catggttcaa 3060
tcaggccttg atggtgatag caccctctca ggaggagacg atgaatctga aaacagcgat 3120
gtggatattg gcgaacctga taccgaggga tatgctatca ctgaccgggg atctgctccc 3180
atctctatgg ggttcagggc ttctgatgtt gaaactgcag aaggagggga gatccacgag 3240
ctcctgaaac tccaatccag aggcaacaac tttccgaagc ttgggaaaac tctcaatgtt 3300
cctccgcccc cgaaccccag tagggccagc acttccgaga cacccattaa aaagggcaca 3360
gacgcgagat tggcctcatt tggaacggag atcgcgtctt tattgacagg tggtgcaacc 3420
caatgtgctc gaaagtcacc ctcggaacca tcagggccag gtgcacctgc ggggaatgtc 3480
cccgagtgtg tgagcaatgc cgcactgata caggagtgga cacccgaatc tggtaccaca 3540
atctccccga gatcccagaa taatgaagaa gggggagact attatgatga tgagctgttc 3600
tccgatgtcc aagacatcaa aacagccttg gccaaaatac acgaggataa tcagaagata 3660
atctccaagc tagaatcatt gctgttattg aagggagaag ttgagtcaat taagaagcag 3720
atcaacaggc aaaatatcag catatccacc ctggaaggac acctctcaag catcatgatt 3780
gccattcctg gacttgggaa ggatcccaac gaccccactg cagatgtcga actcaatccc 3840
gacctgaaac ccatcatagg cagagattca ggccgagcac tggccgaagt tctcaagaag 3900
cccgttgcca gccgacaact ccagggaatg actaatggac ggaccagttc cagaggacag 3960
ctgctgaagg aatttcaact aaagccgatc gggaaaaagg tgagctcagc cgtcgggttt 4020
gttcctgaca ccggccctgc atcacgcagt gtaatccgct ccattataaa atccagccgg 4080
ctagaggagg atcggaagcg ttacctgatg actctccttg atgatatcaa aggagccaac 4140
gatcttgcca agttccacca gatgctgatg aagataataa tgaagtagct acagctcaac 4200
ttacctgcca accccatgcc agtcgaccta attagtacaa cctaaatcca ttataaaaaa 4260
cttaggagca aagtgattgc ctcctaagtt ccacaatgac agagatctac gatttcgaca 4320
agtcggcatg ggacatcaaa gggtcgatcg ctccgataca acctaccacc tacagtgatg 4380
gcaggctggt gccccaggtc agagtcatag atcctggtct aggtgatagg aaggatgaat 4440
gctttatgta catgtttctg ctgggggttg ttgaggacag cgatccccta gggcctccaa 4500
tcgggcgagc attcgggtcc ctgcccttag gtgttggtag atccacagca aaacccgagg 4560
aactcctcaa agaggccact gagcttgaca tagttgttag acgtacagca gggctcaatg 4620
aaaaactggt gttctacaac aacaccccac taaccctcct cacaccttgg agaaaggtcc 4680
taacaacagg gagtgtcttc aatgcaaacc aagtgtgcaa tgcggttaat ctaataccgc 4740
tggacacccc gcagaggttc cgtgttgttt atatgagcat cacccgtctt tcggataacg 4800
ggtattacac cgttcccaga agaatgctgg aattcagatc ggtcaatgca gtggccttca 4860
acctgctagt gacccttagg attgacaagg cgattggccc tgggaagatc atcgacaatg 4920
cagagcaact tcctgaggca acatttatgg tccacatcgg gaacttcagg agaaagaaga 4980
gtgaagtcta ctctgccgat tattgcaaaa tgaaaatcga aaagatgggc ctggtttttg 5040
cacttggtgg gatagggggc accagtcttc acattagaag cacaggcaaa atgagcaaga 5100
ctctccatgc acaactcggg ttcaagaaga ccttatgtta cccactgatg gatatcaatg 5160
aagaccttaa tcggttactc tggaggagca gatgcaagat agtaagaatc caggcagttt 5220
tgcagccatc agttcctcaa gaattccgca tttacgacga cgtgatcata aatgatgacc 5280
aaggactatt caaagttctg tagaccgcag tgcccagcaa tacccgaaaa cgacccccct 5340
cataatgaca gccagaaggc ccggacaaaa aagccccctc cagaagactc cacggaccaa 5400
gcgagaggcc agccagcagc cgacagcaag tgtggacacc aggcggccca agcacagaac 5460
agccccgaca caaggccacc accagccatc ccaatctgcg tcctcctcgt gggacccccg 5520
aggaccaacc ccgaaggtcg ctccgaacac agaccaccaa ccgcatcccc acagctcccg 5580
ggaaaggaac ccccagcaac tggaaggccc ctccccccct cccccaacgc aagaacccca 5640
caaccgaacc gcacaagcga ccgaggtgac ccaaccgcag gcatccgact ccttagacag 5700
atcctctccc cccggcatac taaacaaaac ttagggccaa ggaacacaca cactcgacag 5760
aacccagacc ccggcccgcg gcaccgcgcc cccacccccc gaaaaccaga gggagccccc 5820
aaccaaaccc gccggccccc ccggtgccca caggtaggca caccaacccc cgaccagacc 5880
cagcacccag ccaccgacaa tccaagacgg ggggcccccc ccaaaaaaag gcccccaggg 5940
gccgacagcc agcatcgcga ggaagcacac ccaccccaca cacgaccacg gcaaccgaac 6000
cagagtccag accaccctgg gccaccagct cccagactcg gccatcaccc cgcaaaaagg 6060
aaaggccaca acccgcgcac cccagccccg atccggcggg cagccactca acccgaacca 6120
gcacccaaga gcgatccctg ggggaccccc aaaccgcaaa agacatcagt atcccacagc 6180
ctctccaagt cccccggtct cctcctcttc tcgaagggac caaaagatca atccaccaca 6240
tccgacgaca ctcaattccc cacccccaaa ggagacaccg ggaatcccag aatcaagact 6300
catccagtgt ccatcatggg tctcaaggtg aacgtctctg ccatattcat ggcagtactg 6360
ttaactctcc aaacacccac cggtcaaatc cattggggca atctctctaa gataggggtg 6420
gtagggatag gaagtgcaag ctacaaagtt atgactcgtt ccagccatca atcattggtc 6480
ataaaattaa tgcccaatat aactctcctc aataactgca cgagggtaga gatcgcagaa 6540
tacaggagac tactgagaac agttttggaa ccaattagag atgcacttaa tgcaatgacc 6600
cagaatataa gaccggttca gagtgtagct tcaagtagga gacacaagag atttgcggga 6660
gttgtcctgg caggtgcggc cctaggcgtt gccacagctg ctcagataac agccggcatt 6720
gcacttcacc agtccatgct gaactctcaa gccatcgaca atctgagagc aagcctggaa 6780
actactaatc aggcaattga ggcaatcaga caagcagggc aggagatgat attggctgtt 6840
cagggtgtcc aagactacat caataatgag ctgataccgt ctatgaacca actatcttgt 6900
gatttaatcg gccagaagct agggctcaaa ttgctcagat actatacaga aatcctgtca 6960
ttatttggcc ccagcttacg ggaccccata tctgcggaga tatccatcca ggctttgagc 7020
tatgcgcttg gaggagatat caataaggta ttagaaaagc tcggatacag tggaggtgat 7080
ttactgggca tcttagagag cagaggaata aaggcccgga taactcacgt cgacacagag 7140
tcctacttca ttgtactcag tatagcctat ccgacgctgt ccgagattaa gggggtgatt 7200
gtccaccggc tagagggggt ctcgtacaat ataggctctc aagagtggta taccactgtg 7260
cccaagtatg ttgcaaccca agggtacctt atctcgaatt ttgatgagtc atcgtgtact 7320
ttcatgccag aggggactgt gtgcagccaa aatgccttgt acccgatgag tcctctgctc 7380
caagaatgcc tccgggggtc caccaagtcc tgtgctcgta cactcgtatc cgggtctttt 7440
gggaaccggt tcattttatc acaagggaac ctaatagcca attgtgcatc aatcctctgc 7500
aagtgttaca caacaggaac gatcattaat caagaccctg acaagatcct aacatacatt 7560
gctgccgatc actgcccggt ggtcgaggtg aacggtgtga ccatccaagt cgggagcagg 7620
aggtatccgg acgcggtgta cctgcacaga attgacctcg gtcctcccat atcattggag 7680
aggttggacg tagggacaaa tctggggaat gcaattgcta agttggagga tgccaaggaa 7740
ttgttggagt catcggacca gatattgagg agtatgaaag gtttatcgag cactagcata 7800
gtttacatcc tgattgcagt gtgtcttgga gggttgatag ggatccccgc tttaatatgt 7860
tgctgcaggg ggcgctgtaa caaaaaggga gaacaagttg gtatgtcaag accaggccta 7920
aagcctgatc ttacagggac atcaaaatcc tatgtaaggt cgctctgatc ctctacaact 7980
cttgaaacac agatttccca caagtctcct cttcgtcatc aagcaaccac cgcatccagc 8040
atcaagccca cctgaaattg tctccggctt ccctctggcc gaacgatatc ggtagttaat 8100
taaaacttag ggtgcaagat catccacaat gtcaccacaa cgagaccgaa taaatgcctt 8160
ctacaaagac aacccacatc ctaagggaag taggatagtt attaacagag aacatcttat 8220
gattgataga ccttatgttt tgctggctgt tctattcgtc atgtttctga gcttgatcgg 8280
gttgctagcc attgcaggca ttagactcca tcgtgcagcc atctacaccg cagagatcca 8340
taagagcctc agcaccaatc tagatgtaac taactcgatc gagcatcagg tcaaggacgt 8400
gctgacacca ctcttcaaga tcattggtga tgaagtgggc ctgaggacac ctcagagatt 8460
cactgaccta gtgaaattca tctctgacaa aattaaattc cttaatccgg atagggagta 8520
cgacttcaga gatctcactt ggtgtatcaa cccgccagag agaatcaaat tggattatga 8580
tcaatactgt gcagatgtgg ctgctgaaga actcatgaat gcattggtga actcaactct 8640
actggaggcc agggcaacca atcagttcct agctgtctca aagggaaact gctcagggcc 8700
cactacaatc agaggtcaat tctcaaacat gtcgctgtcc ctgttggact tgtatttaag 8760
tcgaggttac aatgtgtcat ctatagtcac tatgacatcc cagggaatgt acgggggaac 8820
ttacctagtg ggaaagccta atctgagcag taaagggtca gagttgtcac aactgagcat 8880
gcaccgagtg tttgaagtag gggttatcag aaatccgggt ttgggggctc cggtgttcca 8940
tatgacaaac tattttgagc aaccagtcag taatgatttc agcaactgca tggtggcttt 9000
gggggagctt aaattcgcag ccctctgtca cagggaagat tctatcacaa ttccctatca 9060
ggggtcaggg aaaggtgtca gcttccagct cgtcaagcta ggtgtctgga aatccccaac 9120
cgacatgcga tcctgggtcc ccctatcaac ggatgatcca gtgatagata ggctttatct 9180
ctcatctcac agaggtgtta tcgctgacaa tcaagcaaaa tgggctgtcc cgacaacacg 9240
gacagatgac aagttgcgaa tggagacatg cttccagcag gcgtgtaagg gtaaaaacca 9300
agcactctgc gagaatcccg agtgggcacc attgaaggat aacaggattc cttcatacgg 9360
ggtcttgtct gttaatctga gtctgacagt tgagcttaaa atcaaaattg cttcaggatt 9420
cgggccattg atcacacacg gttcagggat ggacctatac aaaaccaacc acaacaatgt 9480
gtattggctg actatcccgc caatgaagaa cctagcctta ggtgtaatca acacattgga 9540
gtggataccg agattcaagg ttagtcccaa cctcttcact gttccaatca aggaagcagg 9600
cgaggactgc catgccccaa catacctacc tgcggaggtg gatggtgatg tcaaactcag 9660
ttccaatctg gtaattctac ctggtcagga tctccaatat gttttggcaa cctacgatac 9720
ttccagggtt gaacatgctg tggtttatta tgtttacagc ccaagccgct cattttctta 9780
cttttatcct tttaggttgc ctataaaggg ggtcccaatc gaattacaag tggaatgctt 9840
cacatgggac aaaaaactct ggtgccgtca cttctgtgtg cttgcggact cagaatctgg 9900
tggacatatc actcactctg ggatggtggg catgggagtc agctgcacag tcactcggga 9960
agatggaacc aatcgcagat agggctgcca gtgaaccgat cacatgatgt cacccagaca 10020
tcaggcatac ccactagtgt gaaatagaca tcagaattaa gaaaaacgta gggtccaagt 10080
ggtttcccgt tatggactcg ctatctgtca accagatctt ataccctgaa gttcacctag 10140
atagcccgat agttaccaat aagatagtag ctatcctgga gtatgctcga gtccctcacg 10200
cttacagcct ggaggaccct acactgtgtc agaacatcaa gcaccgccta aaaaacggat 10260
tctccaacca aatgattata aacaatgtgg aagttgggaa tgtcatcaag tccaagctta 10320
ggagttatcc ggcccactct catattccat atccaaattg taatcaggat ttatttaaca 10380
tagaagacaa agagtcaaca aggaagatcc gtgagctcct aaaaaaggga aattcgctgt 10440
actccaaagt cagtgataag gttttccaat gcctgaggga cactaactca cggcttggcc 10500
taggctccga attgagggag gacatcaagg agaaaattat taacttggga gtttacatgc 10560
acagctccca atggtttgag ccctttctgt tttggtttac agtcaagact gagatgaggt 10620
cagtgattaa atcacaaacc catacttgcc ataggaggag acacacacct gtattcttca 10680
ctggtagttc agttgagctg ttaatctctc gtgaccttgt tgctataatc agtaaggaat 10740
ctcaacatgt atattacctg acgtttgaac tggttttgat gtattgtgat gtcatagagg 10800
ggaggttaat gacagagacc gctatgacca ttgatgctag gtatgcagaa cttctaggaa 10860
gagtcagata catgtggaaa ctgatagatg gtttcttccc tgcactcggg aatccaactt 10920
atcaaattgt agccatgctg gagccacttt cacttgctta cctgcaactg agggatataa 10980
cagtagaact cagaggtgct ttccttaacc actgctttac tgaaatacat gatgttcttg 11040
accaaaacgg gttttctgat gaaggtactt atcatgagtt aattgaagcc ctagattaca 11100
ttttcataac tgatgacata catctgacag gggagatttt ctcatttttc agaagtttcg 11160
gccaccccag acttgaagca gtaacggctg ctgaaaatgt caggaaatac atgaatcagc 11220
ctaaagtcat tgtgtatgag actctgatga aaggtcatgc catattttgt ggaatcataa 11280
tcaacggcta tcgtgacagg cacggaggca gttggccacc cctgaccctc cccctgcatg 11340
ctgcagacac aatccggaat gctcaagctt caggtgaagg gttaacacat gagcagtgcg 11400
ttgataactg gaaatcattt gctggagtga gatttggctg ttttatgcct cttagcctgg 11460
acagtgatct gacaatgtac ctaaaggaca aggcacttgc tgctctccaa agggaatggg 11520
attcagttta cccgaaagag ttcctgcgtt acgatcctcc caagggaacc gggtcacgga 11580
ggcttgtaga tgttttcctt aatgattcga gctttgaccc atatgatatg ataatgtatg 11640
tcgtaagtgg agcctacctc catgaccctg agttcaacct gtcttacagc ctgaaagaaa 11700
aggagatcaa ggaaacaggt agacttttcg ctaaaatgac ttacaaaatg agggcatgcc 11760
aagtgatcgc tgaaaatcta atctcaaacg ggattggcaa gtattttaag gacaatggga 11820
tggccaagga tgagcacgat ttgactaagg cactccacac tctggctgtc tcaggagtcc 11880
ccaaagatct caaagaaagt cacagggggg ggccagtctt aaaaacctac tcccgaagcc 11940
cagtccacac aagtaccagg aacgttaaag cagaaaaagg gtttgtagga ttccctcatg 12000
taattcggca gaatcaagac actgatcatc cggagaatat agaaacctac gagacagtca 12060
gcgcatttat cacgactgat ctcaagaagt actgccttaa ttggagatat gagaccatca 12120
gcttatttgc acagaggcta aatgagattt acggattacc ctcatttttt cagtggctgc 12180
ataagaggct tgaaacctct gtcctctatg taagtgaccc tcattgcccc cccgaccttg 12240
acgcccatgt cccgttatgc aaagtcccca atgaccaaat cttcatcaag taccctatgg 12300
gaggtataga agggtattgt cagaagctgt ggaccatcag caccattccc tacttatacc 12360
tggctgctta tgagagccgg gtaaggattg cttcgttagt gcaaggggac aatcagacca 12420
tagccgtaac aaaaagggta cccagcacat ggcattacaa ccttaagaaa cgggaagctg 12480
ctagagtaac tagagattac tttgtaattc ttaggcaaag gctacatgac attggccatc 12540
acctcaaggc aaatgagaca attgtttcat cacatttttt tgtctattca aaaggaatat 12600
attatgatgg gctacttgtg tcccaatcac tcaagagcat cgcaagatgt gtattctggt 12660
cagagactat agttgatgaa acaagggcgg catgcagtaa tattgctaca acaatggcca 12720
aaagcattga gagaggttat gaccgttatc ttgcatattc cctgaacgtc ctaaaagtga 12780
tacagcaaat tttgatctcc cttggcttca caatcaattc aaccatgacc cgggatgtag 12840
tcatacccct cctcacaaac aacgatctct taataaggat ggcactgttg cccgctccta 12900
ttgggggtat gaattatctg aatatgagca ggctgtttgt cagaaacatc ggtgatccag 12960
taacatcatc aattgctgat ctcaagagaa tgattctcgc atcactaatg cctgaagaga 13020
ccctccatca agtaatgaca caacaaccgg gggactcttc attcctagac tgggctagcg 13080
acccttactc agcaaatctt gtatgcgtcc agagcatcac tagactcctc aagaacataa 13140
ctgcaaggtt tgtcctgatc cacagtccaa acccaatgtt aaaagggtta ttccatgatg 13200
acagcaaaga agaggacgag agactggcgg cattcctcat ggacaggcat attatagtac 13260
ctagggcagc tcatgaaatc ctggatcata gtgtcacagg ggcaagagag tctattgcag 13320
gcatgctaga taccacaaaa ggcctgattc gagccagcat gaggaagggg gggttaacct 13380
ctcgagtgat aaccagattg tccaattatg actatgaaca atttagagca gggatggtgc 13440
tattgacagg aagaaagaga aatgtcctca ttgacaaaga gtcatgttca gtgcagctgg 13500
ctagagccct aagaagccat atgtgggcaa gactagctcg aggacggcct atttacggcc 13560
ttgaggtccc tgatgtacta gaatctatgc gaggccacct tattcggcgt catgagacat 13620
gtgtcatctg cgagtgtgga tcagtcaact acggatggtt ttttgtcccc tcgggttgcc 13680
aactggatga tattgacaag gaaacatcat ccttgagagt cccatatatt ggttctacca 13740
ctgatgagag aacagacatg aagctcgcct tcgtaagagc cccaagtaga tccttgcgat 13800
ctgccgttag aatagcaaca gtgtactcat gggcttacgg tgatgatgat agctcttgga 13860
acgaagcctg gttgttggca aggcaaaggg ccaatgtgag cctggaggag ctaagggtga 13920
tcactcccat ctcgacttcg actaatttag cgcataggtt gagggatcgt agcactcaag 13980
tgaaatactc aggtacatcc cttgtccgag tggcaaggta taccacaatc tccaacgaca 14040
atctctcatt tgtcatatca gataagaagg ttgatactaa ctttatatac caacaaggaa 14100
tgcttctagg gttgggtgtt ttagaaacat tgtttcgact cgagaaagat actggatcat 14160
ctaacacggt attacatctt cacgtcgaaa cagattgttg cgtgatcccg atgatagatc 14220
atcccaggat acccagctcc cgcaagctag agctgagggc agagctatgt accaacccat 14280
tgatatatga taatgcacct ttaattgaca gagatgcaac aaggctatac acccagagcc 14340
ataggaggca ccttgtggaa tttgttacat ggtccacacc ccaactatat cacattctag 14400
ctaagtccac agcactatct atgattgacc tggtaacaaa atttgagaag gaccatatga 14460
atgaaatttc agctctcata ggggatgacg atatcaatag tttcataact gagtttctgc 14520
ttatagagcc aagattattc accatctact tgggccagtg tgcagccatc aattgggcat 14580
ttgatgtaca ttatcataga ccatcaggga aatatcagat gggtgagctg ttgtcttcgt 14640
tcctttctag aatgagcaaa ggagtgttta aggtgcttgt caatgctcta agccacccaa 14700
agatctacaa gaaattctgg cattgtggta ttatagagcc tatccatggt ccttcacttg 14760
atgctcaaaa cttgcacaca actgtgtgca acatggttta cacatgctat atgacctacc 14820
tcgacctgtt gttgaatgaa gagttagaag agttcacatt tcttttgtgt gaaagcgatg 14880
aggatgtagt accggacaga ttcgacaaca tccaggcaaa acacttgtgt gttctggcag 14940
atttgtactg tcaaccaggg acctgcccac cgattcgagg tctaaggccg gtagagaaat 15000
gtgcagttct aaccgatcat atcaaggcag aggctaggtt atctccagca ggatcttcgt 15060
ggaacataaa tccaattatt gtagaccatt actcatgctc tctgacttat ctccgtcgag 15120
gatctatcaa acagataaga ttgagagttg atccaggatt catttttgac gccctcgctg 15180
aggtaaatgt cagtcagcca aaggtcggca gcgacgtcat ggtgagcgtg atcaagcccg 15240
acatgaagat caagctgcgc atggagggcg ccgtgaacgg ccaccccttc gccatcgagg 15300
gcgtgggcct gggcaagccc ttcgagggca agcagagcat ggacctgaag gtgaaggagg 15360
gcggccccct gcccttcgcc tacgacatcc tgaccaccgt gttctgctac ggcaaccgcg 15420
tgttcgccaa gtaccccgag aacatcgtgg actacttcaa gcagagcttc cccgagggct 15480
acagctggga gcgcagcatg aactacgagg acggcggcat ctgcaacgcc accaacgaca 15540
tcaccctgga cggcgactgc tacatctacg agatccgctt cgacggcgtg aacttccccg 15600
ccaacggccc cgtgatgcag aagcgcaccg tgaagtggga gcccagcacc gagaagctgt 15660
acgtgcgcga cggcgtgctg aagggcgacg tgaacatggc cctgagcctg gagggcggcg 15720
gccactaccg ctgcgacttc aagaccacct acaaggccaa gaaggtggtg cagctgcccg 15780
actaccactt cgtggaccac cacatcgaga tcaagagcca cgacaaggac tacagcaacg 15840
tgaacctgca cgagcacgcc gaggcccaca gcgagctgcc ccgccaggcc aagaccggta 15900
attccgctga cggcggcgga ggatcgggtg gtagtggtgg ttcaggagga ggatcgaccc 15960
aaggaggatc catggtgagc gtgatcaagc ccgacatgaa gatcaagctg cgcatggagg 16020
gcgccgtgaa cggccacccc ttcgccatcg agggcgtggg cctgggcaag cccttcgagg 16080
gcaagcagag catggacctg aaggtgaagg agggcggccc cctgcccttc gcctacgaca 16140
tcctgaccac cgtgttctgc tacggcaacc gcgtgttcgc caagtacccc gagaacatcg 16200
tggactactt caagcagagc ttccccgagg gctacagctg ggagcgcagc atgaactacg 16260
aggacggcgg catctgcaac gccaccaacg acatcaccct ggacggcgac tgctacatct 16320
acgagatccg cttcgacggc gtgaacttcc ccgccaacgg ccccgtgatg cagaagcgca 16380
ccgtgaagtg ggagcccagc accgagaatc tgtacgtgcg cgacggcgtg ctgaagggcg 16440
acgtgaacat ggccctgagc ctggagggcg gcggccacta ccgctgcgac ttcaagacca 16500
cctacaaggc caagaaggtg gtgcagctgc ccgactacca cttcgtggac caccacatcg 16560
agatcaagag ccacgacaag gactacagca acgtgaacct gcacgagcac gccgaggccc 16620
acagcgagct gccccgccag gccaaggacg tcatctcaaa tatgagcatc aaggatttca 16680
gacctccaca cgatgatgtt gcaaaattgc tcaaagatat caacacaagc aagcacaatc 16740
ttcccatttc agggggtagt ctcgccaatt atgaaatcca tgctttccgc agaatcgggt 16800
taaactcatc tgcttgctac aaagctgttg agatatcaac attaattagg agatgccttg 16860
agccagggga agacggcttg ttcttgggtg aggggtcggg ttctatgttg atcacttata 16920
aggagatact aaaactaaac aagtgcttct ataatagtgg ggtttccgcc aattctagat 16980
ctggtcaaag ggaattagca ccctatccct ccgaagttgg ccttgtcgaa cacagaatgg 17040
gagtaggtaa tattgtcaag gtgctcttta acgggaggcc cgaagtcacg tgggtaggca 17100
gtatagattg cttcaatttc atagtcagta atatccctac ctctagtgtg gggtttatcc 17160
attcagatat agagacctta cctaacaaag atactataga gaagctagag gaattggcag 17220
ccatcttatc gatggctcta ctccttggca aaataggatc aatactggtg attaagctta 17280
tgcctttcag cggggatttt gttcagggat ttataagcta tgtagggtct cattatagag 17340
aagtgaacct tgtctaccct aggtacagca acttcatatc tactgaatct tatttagtta 17400
tgacagatct caaagctaac cggctaatga atcctgaaaa gatcaagcag cagataattg 17460
aatcatctgt acggacttca cctggactta taggtcacat cctatccatt aagcaactaa 17520
gctgcataca agcaattgtg ggaggcgcgg ttagtagagg tgatatcaac cctattctga 17580
aaaaacttac acctatagag caggtgctga tcagttgcgg gttggcaatt aacggaccta 17640
aactgtgcaa agaattaatc caccatgatg ttgcctcagg gcaagatgga ttgcttaact 17700
ctatactcat cctctacagg gagttggcaa gattcaaaga caaccaaaga agtcaacaag 17760
ggatgttcca cgcttacccc gtattggtaa gtagtaggca acgagaactt gtatctagga 17820
tcactcgcaa attttggggg catattcttc tttactccgg gaacagaaag ttgataaatc 17880
ggtttatcca gaatctcaag tccggttatc tagtactaga cttacaccag aatatcttcg 17940
ttaagaatct atccaagtca gagaaacaga ttattatgac ggggggttta aaacgtgagt 18000
gggtttttaa ggtaacagtc aaggagacca aagaatggta caagttagtc ggatacagcg 18060
ctctgattaa ggattaattg gttgaactcc ggaaccctaa tcctgcccta ggtagttagg 18120
cattatttgc aatatattaa agaaaacttt gaaaatacga agtttctatt cccagctttg 18180
tctggtggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac attccgaggg 18240
gaccgtcccc tcggtaatgg cgaatgggac gcggccgccg cgctcactgg ccgtcgtttt 18300
acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 18360
ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 18420
gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 18480
ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 18540
tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 18600
gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 18660
gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 18720
ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 18780
ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 18840
tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 18900
ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 18960
tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 19020
aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 19080
tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 19140
ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 19200
tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 19260
gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 19320
aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 19380
agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 19440
acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 19500
actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 19560
accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 19620
actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 19680
cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 19740
cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 19800
gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 19860
ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 19920
tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 19980
aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 20040
gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 20100
acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 20160
tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 20220
ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 20280
atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 20340
agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 20400
cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 20460
agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 20520
acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 20580
gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 20640
ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 20700
gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 20760
gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 20820
gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 20880
tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 20940
gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 21000
ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 21060
gccaagcgcg ccagtgaatt gtaatacgac tcactatagg gtgtttggtc tgatgaggcc 21120
gaaaggccga aactccgtaa ggagtc 21146
<210> 14
<211> 20678
<212> DNA
<213> Artificial Sequence
<220>
<223> p(+)MV323-pMag-nMaghigh
<400> 14
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
acgagatggc cacacttttg aggagcttag cattgttcaa aagaaacaag gacaaaccac 1020
ccattacatc aggatccggt ggagccatca gaggaatcaa acacattatt atagtaccaa 1080
ttcctggaga ttcctcaatt accactcgat ccagactact ggaccggttg gtcaggttaa 1140
ttggaaaccc ggatgtgagc gggcccaaac taacaggggc actaataggt atattatcct 1200
tatttgtgga gtctccaggt caattgattc agaggatcac cgatgaccct gacgttagca 1260
tcaggctgtt agaggttgtt cagagtgacc agtcacaatc tggccttacc ttcgcatcaa 1320
gaggtaccaa catggaggat gaggcggacc aatacttttc acatgatgat ccaagcagta 1380
gtgatcaatc caggtccgga tggttcgaga acaaggaaat ctcagatatt gaagtgcaag 1440
accctgaggg attcaacatg attctgggta ccattctagc ccagatctgg gtcttgctcg 1500
caaaggcggt tacggcccca gacacggcag ctgattcgga gctaagaagg tggataaagt 1560
acacccaaca aagaagggta gttggtgaat ttagattgga gagaaaatgg ttggatgtgg 1620
tgaggaacag gattgccgag gacctctctt tacgccgatt catggtggct ctaatcctgg 1680
atatcaagag gacacccggg aacaaaccta ggattgctga aatgatatgt gacattgata 1740
catatatcgt agaggcagga ttagccagtt ttatcctgac tattaagttt gggatagaaa 1800
ctatgtatcc tgctcttgga ctgcatgaat ttgctggtga gttatccaca cttgagtcct 1860
tgatgaatct ttaccagcaa atgggagaaa ctgcacccta catggtaatc ctagagaact 1920
caattcagaa caagttcagt gcaggatcat accctctgct ctggagctat gccatgggag 1980
taggagtgga acttgaaaac tccatgggag gtttgaactt tggtcgatct tactttgatc 2040
cagcatattt tagattaggg caagagatgg tgaggaggtc agctggaaag gtcagttcca 2100
cattggcatc cgaactcggt atcactgccg aggatgcaag gcttgtttca gagattgcaa 2160
tgcatactac tgaggacagg atcagtagag cggtcggacc cagacaagcc caagtgtcat 2220
ttctacacgg tgatcaaagt gagaatgagc taccaggatt ggggggcaag gaagatagga 2280
gggtcaaaca gggtcgggga gaagccaggg agagctacag agaaaccggg tccagcagag 2340
caagtgatgc gagagctgcc catcctccaa ccagcatgcc cctagacatt gacactgcat 2400
cggagtcagg ccaagatccg caggacagtc gaaggtcagc tgacgccctg ctcaggctgc 2460
aagccatggc aggaatcttg gaagaacaag gctcagacac ggacacccct agggtataca 2520
atgacagaga tcttctagac taggtgcgag aggccgagga ccagaacaac atccgcctac 2580
cctccatcat tgttataaaa aacttaggaa ccaggtccac acagccgcca gccaaccaac 2640
catccactcc cacgactgga gccgatggca gaagagcagg cacgccatgt caaaaacgga 2700
ctggaatgca tccgggctct caaggccgag cccatcggct cactggccgt cgaggaagcc 2760
atggcagcat ggtcagaaat atcagacaac ccaggacagg accgagccac ctgcaaggaa 2820
gaggaggcag gcagttcggg tctcagcaaa ccatgcctct cagcaattgg atcaactgaa 2880
ggcggtgcac ctcgcatccg cggtcaggga tctggagaaa gcgatgacga cgctgaaact 2940
ttgggaatcc cctcaagaaa tctccaggca tcaagcactg ggttacagtg ttatcatgtt 3000
tatgatcaca gcggtgaagc ggttaaggga atccaagatg ctgactctat catggttcaa 3060
tcaggccttg atggtgatag caccctctca ggaggagacg atgaatctga aaacagcgat 3120
gtggatattg gcgaacctga taccgaggga tatgctatca ctgaccgggg atctgctccc 3180
atctctatgg ggttcagggc ttctgatgtt gaaactgcag aaggagggga gatccacgag 3240
ctcctgaaac tccaatccag aggcaacaac tttccgaagc ttgggaaaac tctcaatgtt 3300
cctccgcccc cgaaccccag tagggccagc acttccgaga cacccattaa aaagggcaca 3360
gacgcgagat tggcctcatt tggaacggag atcgcgtctt tattgacagg tggtgcaacc 3420
caatgtgctc gaaagtcacc ctcggaacca tcagggccag gtgcacctgc ggggaatgtc 3480
cccgagtgtg tgagcaatgc cgcactgata caggagtgga cacccgaatc tggtaccaca 3540
atctccccga gatcccagaa taatgaagaa gggggagact attatgatga tgagctgttc 3600
tccgatgtcc aagacatcaa aacagccttg gccaaaatac acgaggataa tcagaagata 3660
atctccaagc tagaatcatt gctgttattg aagggagaag ttgagtcaat taagaagcag 3720
atcaacaggc aaaatatcag catatccacc ctggaaggac acctctcaag catcatgatt 3780
gccattcctg gacttgggaa ggatcccaac gaccccactg cagatgtcga actcaatccc 3840
gacctgaaac ccatcatagg cagagattca ggccgagcac tggccgaagt tctcaagaag 3900
cccgttgcca gccgacaact ccagggaatg actaatggac ggaccagttc cagaggacag 3960
ctgctgaagg aatttcaact aaagccgatc gggaaaaagg tgagctcagc cgtcgggttt 4020
gttcctgaca ccggccctgc atcacgcagt gtaatccgct ccattataaa atccagccgg 4080
ctagaggagg atcggaagcg ttacctgatg actctccttg atgatatcaa aggagccaac 4140
gatcttgcca agttccacca gatgctgatg aagataataa tgaagtagct acagctcaac 4200
ttacctgcca accccatgcc agtcgaccta attagtacaa cctaaatcca ttataaaaaa 4260
cttaggagca aagtgattgc ctcctaagtt ccacaatgac agagatctac gatttcgaca 4320
agtcggcatg ggacatcaaa gggtcgatcg ctccgataca acctaccacc tacagtgatg 4380
gcaggctggt gccccaggtc agagtcatag atcctggtct aggtgatagg aaggatgaat 4440
gctttatgta catgtttctg ctgggggttg ttgaggacag cgatccccta gggcctccaa 4500
tcgggcgagc attcgggtcc ctgcccttag gtgttggtag atccacagca aaacccgagg 4560
aactcctcaa agaggccact gagcttgaca tagttgttag acgtacagca gggctcaatg 4620
aaaaactggt gttctacaac aacaccccac taaccctcct cacaccttgg agaaaggtcc 4680
taacaacagg gagtgtcttc aatgcaaacc aagtgtgcaa tgcggttaat ctaataccgc 4740
tggacacccc gcagaggttc cgtgttgttt atatgagcat cacccgtctt tcggataacg 4800
ggtattacac cgttcccaga agaatgctgg aattcagatc ggtcaatgca gtggccttca 4860
acctgctagt gacccttagg attgacaagg cgattggccc tgggaagatc atcgacaatg 4920
cagagcaact tcctgaggca acatttatgg tccacatcgg gaacttcagg agaaagaaga 4980
gtgaagtcta ctctgccgat tattgcaaaa tgaaaatcga aaagatgggc ctggtttttg 5040
cacttggtgg gatagggggc accagtcttc acattagaag cacaggcaaa atgagcaaga 5100
ctctccatgc acaactcggg ttcaagaaga ccttatgtta cccactgatg gatatcaatg 5160
aagaccttaa tcggttactc tggaggagca gatgcaagat agtaagaatc caggcagttt 5220
tgcagccatc agttcctcaa gaattccgca tttacgacga cgtgatcata aatgatgacc 5280
aaggactatt caaagttctg tagaccgcag tgcccagcaa tacccgaaaa cgacccccct 5340
cataatgaca gccagaaggc ccggacaaaa aagccccctc cagaagactc cacggaccaa 5400
gcgagaggcc agccagcagc cgacagcaag tgtggacacc aggcggccca agcacagaac 5460
agccccgaca caaggccacc accagccatc ccaatctgcg tcctcctcgt gggacccccg 5520
aggaccaacc ccgaaggtcg ctccgaacac agaccaccaa ccgcatcccc acagctcccg 5580
ggaaaggaac ccccagcaac tggaaggccc ctccccccct cccccaacgc aagaacccca 5640
caaccgaacc gcacaagcga ccgaggtgac ccaaccgcag gcatccgact ccttagacag 5700
atcctctccc cccggcatac taaacaaaac ttagggccaa ggaacacaca cactcgacag 5760
aacccagacc ccggcccgcg gcaccgcgcc cccacccccc gaaaaccaga gggagccccc 5820
aaccaaaccc gccggccccc ccggtgccca caggtaggca caccaacccc cgaccagacc 5880
cagcacccag ccaccgacaa tccaagacgg ggggcccccc ccaaaaaaag gcccccaggg 5940
gccgacagcc agcatcgcga ggaagcacac ccaccccaca cacgaccacg gcaaccgaac 6000
cagagtccag accaccctgg gccaccagct cccagactcg gccatcaccc cgcaaaaagg 6060
aaaggccaca acccgcgcac cccagccccg atccggcggg cagccactca acccgaacca 6120
gcacccaaga gcgatccctg ggggaccccc aaaccgcaaa agacatcagt atcccacagc 6180
ctctccaagt cccccggtct cctcctcttc tcgaagggac caaaagatca atccaccaca 6240
tccgacgaca ctcaattccc cacccccaaa ggagacaccg ggaatcccag aatcaagact 6300
catccagtgt ccatcatggg tctcaaggtg aacgtctctg ccatattcat ggcagtactg 6360
ttaactctcc aaacacccac cggtcaaatc cattggggca atctctctaa gataggggtg 6420
gtagggatag gaagtgcaag ctacaaagtt atgactcgtt ccagccatca atcattggtc 6480
ataaaattaa tgcccaatat aactctcctc aataactgca cgagggtaga gatcgcagaa 6540
tacaggagac tactgagaac agttttggaa ccaattagag atgcacttaa tgcaatgacc 6600
cagaatataa gaccggttca gagtgtagct tcaagtagga gacacaagag atttgcggga 6660
gttgtcctgg caggtgcggc cctaggcgtt gccacagctg ctcagataac agccggcatt 6720
gcacttcacc agtccatgct gaactctcaa gccatcgaca atctgagagc aagcctggaa 6780
actactaatc aggcaattga ggcaatcaga caagcagggc aggagatgat attggctgtt 6840
cagggtgtcc aagactacat caataatgag ctgataccgt ctatgaacca actatcttgt 6900
gatttaatcg gccagaagct agggctcaaa ttgctcagat actatacaga aatcctgtca 6960
ttatttggcc ccagcttacg ggaccccata tctgcggaga tatccatcca ggctttgagc 7020
tatgcgcttg gaggagatat caataaggta ttagaaaagc tcggatacag tggaggtgat 7080
ttactgggca tcttagagag cagaggaata aaggcccgga taactcacgt cgacacagag 7140
tcctacttca ttgtactcag tatagcctat ccgacgctgt ccgagattaa gggggtgatt 7200
gtccaccggc tagagggggt ctcgtacaat ataggctctc aagagtggta taccactgtg 7260
cccaagtatg ttgcaaccca agggtacctt atctcgaatt ttgatgagtc atcgtgtact 7320
ttcatgccag aggggactgt gtgcagccaa aatgccttgt acccgatgag tcctctgctc 7380
caagaatgcc tccgggggtc caccaagtcc tgtgctcgta cactcgtatc cgggtctttt 7440
gggaaccggt tcattttatc acaagggaac ctaatagcca attgtgcatc aatcctctgc 7500
aagtgttaca caacaggaac gatcattaat caagaccctg acaagatcct aacatacatt 7560
gctgccgatc actgcccggt ggtcgaggtg aacggtgtga ccatccaagt cgggagcagg 7620
aggtatccgg acgcggtgta cctgcacaga attgacctcg gtcctcccat atcattggag 7680
aggttggacg tagggacaaa tctggggaat gcaattgcta agttggagga tgccaaggaa 7740
ttgttggagt catcggacca gatattgagg agtatgaaag gtttatcgag cactagcata 7800
gtttacatcc tgattgcagt gtgtcttgga gggttgatag ggatccccgc tttaatatgt 7860
tgctgcaggg ggcgctgtaa caaaaaggga gaacaagttg gtatgtcaag accaggccta 7920
aagcctgatc ttacagggac atcaaaatcc tatgtaaggt cgctctgatc ctctacaact 7980
cttgaaacac agatttccca caagtctcct cttcgtcatc aagcaaccac cgcatccagc 8040
atcaagccca cctgaaattg tctccggctt ccctctggcc gaacgatatc ggtagttaat 8100
taaaacttag ggtgcaagat catccacaat gtcaccacaa cgagaccgaa taaatgcctt 8160
ctacaaagac aacccacatc ctaagggaag taggatagtt attaacagag aacatcttat 8220
gattgataga ccttatgttt tgctggctgt tctattcgtc atgtttctga gcttgatcgg 8280
gttgctagcc attgcaggca ttagactcca tcgtgcagcc atctacaccg cagagatcca 8340
taagagcctc agcaccaatc tagatgtaac taactcgatc gagcatcagg tcaaggacgt 8400
gctgacacca ctcttcaaga tcattggtga tgaagtgggc ctgaggacac ctcagagatt 8460
cactgaccta gtgaaattca tctctgacaa aattaaattc cttaatccgg atagggagta 8520
cgacttcaga gatctcactt ggtgtatcaa cccgccagag agaatcaaat tggattatga 8580
tcaatactgt gcagatgtgg ctgctgaaga actcatgaat gcattggtga actcaactct 8640
actggaggcc agggcaacca atcagttcct agctgtctca aagggaaact gctcagggcc 8700
cactacaatc agaggtcaat tctcaaacat gtcgctgtcc ctgttggact tgtatttaag 8760
tcgaggttac aatgtgtcat ctatagtcac tatgacatcc cagggaatgt acgggggaac 8820
ttacctagtg ggaaagccta atctgagcag taaagggtca gagttgtcac aactgagcat 8880
gcaccgagtg tttgaagtag gggttatcag aaatccgggt ttgggggctc cggtgttcca 8940
tatgacaaac tattttgagc aaccagtcag taatgatttc agcaactgca tggtggcttt 9000
gggggagctt aaattcgcag ccctctgtca cagggaagat tctatcacaa ttccctatca 9060
ggggtcaggg aaaggtgtca gcttccagct cgtcaagcta ggtgtctgga aatccccaac 9120
cgacatgcga tcctgggtcc ccctatcaac ggatgatcca gtgatagata ggctttatct 9180
ctcatctcac agaggtgtta tcgctgacaa tcaagcaaaa tgggctgtcc cgacaacacg 9240
gacagatgac aagttgcgaa tggagacatg cttccagcag gcgtgtaagg gtaaaaacca 9300
agcactctgc gagaatcccg agtgggcacc attgaaggat aacaggattc cttcatacgg 9360
ggtcttgtct gttaatctga gtctgacagt tgagcttaaa atcaaaattg cttcaggatt 9420
cgggccattg atcacacacg gttcagggat ggacctatac aaaaccaacc acaacaatgt 9480
gtattggctg actatcccgc caatgaagaa cctagcctta ggtgtaatca acacattgga 9540
gtggataccg agattcaagg ttagtcccaa cctcttcact gttccaatca aggaagcagg 9600
cgaggactgc catgccccaa catacctacc tgcggaggtg gatggtgatg tcaaactcag 9660
ttccaatctg gtaattctac ctggtcagga tctccaatat gttttggcaa cctacgatac 9720
ttccagggtt gaacatgctg tggtttatta tgtttacagc ccaagccgct cattttctta 9780
cttttatcct tttaggttgc ctataaaggg ggtcccaatc gaattacaag tggaatgctt 9840
cacatgggac aaaaaactct ggtgccgtca cttctgtgtg cttgcggact cagaatctgg 9900
tggacatatc actcactctg ggatggtggg catgggagtc agctgcacag tcactcggga 9960
agatggaacc aatcgcagat agggctgcca gtgaaccgat cacatgatgt cacccagaca 10020
tcaggcatac ccactagtgt gaaatagaca tcagaattaa gaaaaacgta gggtccaagt 10080
ggtttcccgt tatggactcg ctatctgtca accagatctt ataccctgaa gttcacctag 10140
atagcccgat agttaccaat aagatagtag ctatcctgga gtatgctcga gtccctcacg 10200
cttacagcct ggaggaccct acactgtgtc agaacatcaa gcaccgccta aaaaacggat 10260
tctccaacca aatgattata aacaatgtgg aagttgggaa tgtcatcaag tccaagctta 10320
ggagttatcc ggcccactct catattccat atccaaattg taatcaggat ttatttaaca 10380
tagaagacaa agagtcaaca aggaagatcc gtgagctcct aaaaaaggga aattcgctgt 10440
actccaaagt cagtgataag gttttccaat gcctgaggga cactaactca cggcttggcc 10500
taggctccga attgagggag gacatcaagg agaaaattat taacttggga gtttacatgc 10560
acagctccca atggtttgag ccctttctgt tttggtttac agtcaagact gagatgaggt 10620
cagtgattaa atcacaaacc catacttgcc ataggaggag acacacacct gtattcttca 10680
ctggtagttc agttgagctg ttaatctctc gtgaccttgt tgctataatc agtaaggaat 10740
ctcaacatgt atattacctg acgtttgaac tggttttgat gtattgtgat gtcatagagg 10800
ggaggttaat gacagagacc gctatgacca ttgatgctag gtatgcagaa cttctaggaa 10860
gagtcagata catgtggaaa ctgatagatg gtttcttccc tgcactcggg aatccaactt 10920
atcaaattgt agccatgctg gagccacttt cacttgctta cctgcaactg agggatataa 10980
cagtagaact cagaggtgct ttccttaacc actgctttac tgaaatacat gatgttcttg 11040
accaaaacgg gttttctgat gaaggtactt atcatgagtt aattgaagcc ctagattaca 11100
ttttcataac tgatgacata catctgacag gggagatttt ctcatttttc agaagtttcg 11160
gccaccccag acttgaagca gtaacggctg ctgaaaatgt caggaaatac atgaatcagc 11220
ctaaagtcat tgtgtatgag actctgatga aaggtcatgc catattttgt ggaatcataa 11280
tcaacggcta tcgtgacagg cacggaggca gttggccacc cctgaccctc cccctgcatg 11340
ctgcagacac aatccggaat gctcaagctt caggtgaagg gttaacacat gagcagtgcg 11400
ttgataactg gaaatcattt gctggagtga gatttggctg ttttatgcct cttagcctgg 11460
acagtgatct gacaatgtac ctaaaggaca aggcacttgc tgctctccaa agggaatggg 11520
attcagttta cccgaaagag ttcctgcgtt acgatcctcc caagggaacc gggtcacgga 11580
ggcttgtaga tgttttcctt aatgattcga gctttgaccc atatgatatg ataatgtatg 11640
tcgtaagtgg agcctacctc catgaccctg agttcaacct gtcttacagc ctgaaagaaa 11700
aggagatcaa ggaaacaggt agacttttcg ctaaaatgac ttacaaaatg agggcatgcc 11760
aagtgatcgc tgaaaatcta atctcaaacg ggattggcaa gtattttaag gacaatggga 11820
tggccaagga tgagcacgat ttgactaagg cactccacac tctggctgtc tcaggagtcc 11880
ccaaagatct caaagaaagt cacagggggg ggccagtctt aaaaacctac tcccgaagcc 11940
cagtccacac aagtaccagg aacgttaaag cagaaaaagg gtttgtagga ttccctcatg 12000
taattcggca gaatcaagac actgatcatc cggagaatat agaaacctac gagacagtca 12060
gcgcatttat cacgactgat ctcaagaagt actgccttaa ttggagatat gagaccatca 12120
gcttatttgc acagaggcta aatgagattt acggattacc ctcatttttt cagtggctgc 12180
ataagaggct tgaaacctct gtcctctatg taagtgaccc tcattgcccc cccgaccttg 12240
acgcccatgt cccgttatgc aaagtcccca atgaccaaat cttcatcaag taccctatgg 12300
gaggtataga agggtattgt cagaagctgt ggaccatcag caccattccc tacttatacc 12360
tggctgctta tgagagcggg gtaaggattg cttcgttagt gcaaggggac aatcagacca 12420
tagccgtaac aaaaagggta cccagcacat ggccttacaa ccttaagaaa cgggaagctg 12480
ctagagtaac tagagattac tttgtaattc ttaggcaaag gctacatgac attggccatc 12540
acctcaaggc aaatgagaca attgtttcat cacatttttt tgtctattca aaaggaatat 12600
attatgatgg gctacttgtg tcccaatcac tcaagagcat cgcaagatgt gtattctggt 12660
cagagactat agttgatgaa acaagggcgg catgcagtaa tattgctaca acaatggcca 12720
aaagcattga gagaggttat gaccgttatc ttgcatattc cctgaacgtc ctaaaagtga 12780
tacagcaaat tttgatctcc cttggcttca caatcaattc aaccatgacc cgggatgtag 12840
tcatacccct cctcacaaac aacgatctct taataaggat ggcactgttg cccgctccta 12900
ttgggggtat gaattatctg aatatgagca ggctgtttgt cagaaacatc ggtgatccag 12960
taacatcatc aattgctgat ctcaagagaa tgattctcgc atcactaatg cctgaagaga 13020
ccctccatca agtaatgaca caacaaccgg gggactcttc attcctagac tgggctagcg 13080
acccttactc agcaaatctt gtatgcgtcc agagcatcac tagactcctc aagaacataa 13140
ctgcaaggtt tgtcctgatc cacagtccaa acccaatgtt aaaagggtta ttccatgatg 13200
acagcaaaga agaggacgag agactggcgg cattcctcat ggacaggcat attatagtac 13260
ctagggcagc tcatgaaatc ctggatcata gtgtcacagg ggcaagagag tctattgcag 13320
gcatgctaga taccacaaaa ggcctgattc gagccagcat gaggaagggg gggttaacct 13380
ctcgagtgat aaccagattg tccaattatg actatgaaca atttagagca gggatggtgc 13440
tattgacagg aagaaagaga aatgtcctca ttgacaaaga gtcatgttca gtgcagctgg 13500
ctagagccct aagaagccat atgtgggcaa gactagctcg aggacggcct atttacggcc 13560
ttgaggtccc tgatgtacta gaatctatgc gaggccacct tattcggcgt catgagacat 13620
gtgtcatctg cgagtgtgga tcagtcaact acggatggtt ttttgtcccc tcgggttgcc 13680
aactggatga tattgacaag gaaacatcat ccttgagagt cccatatatt ggttctacca 13740
ctgatgagag aacagacatg aagctcgcct tcgtaagagc cccaagtaga tccttgcgat 13800
ctgccgttag aatagcaaca gtgtactcat gggcttacgg tgatgatgat agctcttgga 13860
acgaagcctg gttgttggca aggcaaaggg ccaatgtgag cctggaggag ctaagggtga 13920
tcactcccat ctcgacttcg actaatttag cgcataggtt gagggatcgt agcactcaag 13980
tgaaatactc aggtacatcc cttgtccgag tggcaaggta taccacaatc tccaacgaca 14040
atctctcatt tgtcatatca gataagaagg ttgatactaa ctttatatac caacaaggaa 14100
tgcttctagg gttgggtgtt ttagaaacat tgtttcgact cgagaaagat actggatcat 14160
ctaacacggt attacatctt cacgtcgaaa cagattgttg cgtgatcccg atgatagatc 14220
atcccaggat acccagctcc cgcaagctag agctgagggc agagctatgt accaacccat 14280
tgatatatga taatgcacct ttaattgaca gagatgcaac aaggctatac acccagagcc 14340
ataggaggca ccttgtggaa tttgttacat ggtccacacc ccaactatat cacattctag 14400
ctaagtccac agcactatct atgattgacc tggtaacaaa atttgagaag gaccatatga 14460
atgaaatttc agctctcata ggggatgacg atatcaatag tttcataact gagtttctgc 14520
ttatagagcc aagattattc accatctact tgggccagtg tgcagccatc aattgggcat 14580
ttgatgtaca ttatcataga ccatcaggga aatatcagat gggtgagctg ttgtcttcgt 14640
tcctttctag aatgagcaaa ggagtgttta aggtgcttgt caatgctcta agccacccaa 14700
agatctacaa gaaattctgg cattgtggta ttatagagcc tatccatggt ccttcacttg 14760
atgctcaaaa cttgcacaca actgtgtgca acatggttta cacatgctat atgacctacc 14820
tcgacctgtt gttgaatgaa gagttagaag agttcacatt tcttttgtgt gaaagcgatg 14880
aggatgtagt accggacaga ttcgacaaca tccaggcaaa acacttgtgt gttctggcag 14940
atttgtactg tcaaccaggg acctgcccac cgattcgagg tctaaggccg gtagagaaat 15000
gtgcagttct aaccgatcat atcaaggcag aggctaggtt atctccagca ggatcttcgt 15060
ggaacataaa tccaattatt gtagaccatt actcatgctc tctgacttat ctccgtcgag 15120
gatctatcaa acagataaga ttgagagttg atccaggatt catttttgac gccctcgctg 15180
aggtaaatgt cagtcagcca aaggtcggca gcgacgtcag ccatactctt tatgcccccg 15240
gtggatatga cattatggga tatctgaggc agatcaggaa ccggccaaac ccgcaggtgg 15300
aactgggccc cgtggataca tcctgcgcct tgattctttg tgacctgaaa cagaaagaca 15360
ccccgatagt ttacgcgagt gaagccttcc tctacatgac aggttacagc aacgcagagg 15420
tgctgggccg gaattgccgg tttctgcaaa gccctgacgg catggtgaag cccaagagca 15480
cccggaagta cgtggatagt aacacaatca atactatgcg caaggcaatc gacaggaatg 15540
ccgaggtgca ggttgaagta gtcaatttta aaaagaatgg acagcgattt gttaatttcc 15600
tgactatgat acctgttagg gacgaaacag gcgagtatcg atactctatg ggattccagt 15660
gcgaaacaga aaccggtaat tccgctgacg gcggcggagg atcgggtggt agtggtggtt 15720
caggaggagg atcgacccaa ggaggatcca tgcatactct ttatgccccc ggtggatatg 15780
acattatggg atatctggac cagatcggca accggccaaa cccgcaggtg gaactgggcc 15840
ccgtggatac atcctgcgcc ttgattcttt gtgacctgaa acagaaagac accccgatag 15900
tttacgcgag tgaagccttc ctctacatga caggttacag caacgcagag gtgctgggcc 15960
ggaattgccg gtttctgcaa agccctgacg gcatggtgaa gcccaagagc acccggaagt 16020
acgtggatag taacacaatc aatactatcc gcaaggcaat cgacaggaat gccgaggtgc 16080
aggttgaagt agtcaatttt aaaaagaatg gacagcgatt tgttaatttc ctgactatca 16140
tacctgttag ggacgaaaca ggcgagtatc gatactctat gggattccag tgcgaaacag 16200
aatatccgta cgacgtccca gattacgcgg gcatctcaaa tatgagcatc aaggatttca 16260
gacctccaca cgatgatgtt gcaaaattgc tcaaagatat caacacaagc aagcacaatc 16320
ttcccatttc agggggtagt ctcgccaatt atgaaatcca tgctttccgc agaatcgggt 16380
taaactcatc tgcttgctac aaagctgttg agatatcaac attaattagg agatgccttg 16440
agccagggga agacggcttg ttcttgggtg aggggtcggg ttctatgttg atcacttata 16500
aggagatact aaaactaaac aagtgcttct ataatagtgg ggtttccgcc aattctagat 16560
ctggtcaaag ggaattagca ccctatccct ccgaagttgg ccttgtcgaa cacagaatgg 16620
gagtaggtaa tattgtcaag gtgctcttta acgggaggcc cgaagtcacg tgggtaggca 16680
gtatagattg cttcaatttc atagtcagta atatccctac ctctagtgtg gggtttatcc 16740
attcagatat agagacctta cctaacaaag atactataga gaagctagag gaattggcag 16800
ccatcttatc gatggctcta ctccttggca aaataggatc aatactggtg attaagctta 16860
tgcctttcag cggggatttt gttcagggat ttataagcta tgtagggtct cattatagag 16920
aagtgaacct tgtctaccct aggtacagca acttcatatc tactgaatct tatttagtta 16980
tgacagatct caaagctaac cggctaatga atcctgaaaa gatcaagcag cagataattg 17040
aatcatctgt acggacttca cctggactta taggtcacat cctatccatt aagcaactaa 17100
gctgcataca agcaattgtg ggaggcgcgg ttagtagagg tgatatcaac cctattctga 17160
aaaaacttac acctatagag caggtgctga tcagttgcgg gttggcaatt aacggaccta 17220
aactgtgcaa agaattaatc caccatgatg ttgcctcagg gcaagatgga ttgcttaact 17280
ctatactcat cctctacagg gagttggcaa gattcaaaga caaccaaaga agtcaacaag 17340
ggatgttcca cgcttacccc gtattggtaa gtagtaggca acgagaactt gtatctagga 17400
tcactcgcaa attttggggg catattcttc tttactccgg gaacagaaag ttgataaatc 17460
ggtttatcca gaatctcaag tccggttatc tagtactaga cttacaccag aatatcttcg 17520
ttaagaatct atccaagtca gagaaacaga ttattatgac ggggggttta aaacgtgagt 17580
gggtttttaa ggtaacagtc aaggagacca aagaatggta caagttagtc ggatacagcg 17640
ctctgattaa ggattaattg gttgaactcc ggaaccctaa tcctgcccta ggtagttagg 17700
cattatttgc aatatattaa agaaaacttt gaaaatacga agtttctatt cccagctttg 17760
tctggtggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac attccgaggg 17820
gaccgtcccc tcggtaatgg cgaatgggac gcggccgccg cgctcactgg ccgtcgtttt 17880
acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 17940
ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 18000
gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 18060
ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 18120
tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 18180
gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 18240
gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 18300
ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 18360
ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 18420
tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 18480
ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 18540
tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 18600
aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 18660
tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 18720
ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 18780
tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 18840
gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 18900
aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 18960
agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 19020
acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 19080
actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 19140
accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 19200
actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 19260
cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 19320
cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 19380
gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 19440
ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 19500
tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 19560
aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 19620
gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 19680
acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 19740
tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 19800
ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 19860
atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 19920
agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 19980
cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 20040
agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 20100
acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 20160
gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 20220
ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 20280
gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 20340
gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 20400
gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 20460
tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 20520
gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 20580
ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 20640
gccaagcgcg ccagtgaatt gtaatacgac tcactata 20678
<210> 15
<211> 22118
<212> DNA
<213> Artificial Sequence
<220>
<223> p(+)MV323-CRY2-CIB1N
<400> 15
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
acgagatggc cacacttttg aggagcttag cattgttcaa aagaaacaag gacaaaccac 1020
ccattacatc aggatccggt ggagccatca gaggaatcaa acacattatt atagtaccaa 1080
ttcctggaga ttcctcaatt accactcgat ccagactact ggaccggttg gtcaggttaa 1140
ttggaaaccc ggatgtgagc gggcccaaac taacaggggc actaataggt atattatcct 1200
tatttgtgga gtctccaggt caattgattc agaggatcac cgatgaccct gacgttagca 1260
tcaggctgtt agaggttgtt cagagtgacc agtcacaatc tggccttacc ttcgcatcaa 1320
gaggtaccaa catggaggat gaggcggacc aatacttttc acatgatgat ccaagcagta 1380
gtgatcaatc caggtccgga tggttcgaga acaaggaaat ctcagatatt gaagtgcaag 1440
accctgaggg attcaacatg attctgggta ccattctagc ccagatctgg gtcttgctcg 1500
caaaggcggt tacggcccca gacacggcag ctgattcgga gctaagaagg tggataaagt 1560
acacccaaca aagaagggta gttggtgaat ttagattgga gagaaaatgg ttggatgtgg 1620
tgaggaacag gattgccgag gacctctctt tacgccgatt catggtggct ctaatcctgg 1680
atatcaagag gacacccggg aacaaaccta ggattgctga aatgatatgt gacattgata 1740
catatatcgt agaggcagga ttagccagtt ttatcctgac tattaagttt gggatagaaa 1800
ctatgtatcc tgctcttgga ctgcatgaat ttgctggtga gttatccaca cttgagtcct 1860
tgatgaatct ttaccagcaa atgggagaaa ctgcacccta catggtaatc ctagagaact 1920
caattcagaa caagttcagt gcaggatcat accctctgct ctggagctat gccatgggag 1980
taggagtgga acttgaaaac tccatgggag gtttgaactt tggtcgatct tactttgatc 2040
cagcatattt tagattaggg caagagatgg tgaggaggtc agctggaaag gtcagttcca 2100
cattggcatc cgaactcggt atcactgccg aggatgcaag gcttgtttca gagattgcaa 2160
tgcatactac tgaggacagg atcagtagag cggtcggacc cagacaagcc caagtgtcat 2220
ttctacacgg tgatcaaagt gagaatgagc taccaggatt ggggggcaag gaagatagga 2280
gggtcaaaca gggtcgggga gaagccaggg agagctacag agaaaccggg tccagcagag 2340
caagtgatgc gagagctgcc catcctccaa ccagcatgcc cctagacatt gacactgcat 2400
cggagtcagg ccaagatccg caggacagtc gaaggtcagc tgacgccctg ctcaggctgc 2460
aagccatggc aggaatcttg gaagaacaag gctcagacac ggacacccct agggtataca 2520
atgacagaga tcttctagac taggtgcgag aggccgagga ccagaacaac atccgcctac 2580
cctccatcat tgttataaaa aacttaggaa ccaggtccac acagccgcca gccaaccaac 2640
catccactcc cacgactgga gccgatggca gaagagcagg cacgccatgt caaaaacgga 2700
ctggaatgca tccgggctct caaggccgag cccatcggct cactggccgt cgaggaagcc 2760
atggcagcat ggtcagaaat atcagacaac ccaggacagg accgagccac ctgcaaggaa 2820
gaggaggcag gcagttcggg tctcagcaaa ccatgcctct cagcaattgg atcaactgaa 2880
ggcggtgcac ctcgcatccg cggtcaggga tctggagaaa gcgatgacga cgctgaaact 2940
ttgggaatcc cctcaagaaa tctccaggca tcaagcactg ggttacagtg ttatcatgtt 3000
tatgatcaca gcggtgaagc ggttaaggga atccaagatg ctgactctat catggttcaa 3060
tcaggccttg atggtgatag caccctctca ggaggagacg atgaatctga aaacagcgat 3120
gtggatattg gcgaacctga taccgaggga tatgctatca ctgaccgggg atctgctccc 3180
atctctatgg ggttcagggc ttctgatgtt gaaactgcag aaggagggga gatccacgag 3240
ctcctgaaac tccaatccag aggcaacaac tttccgaagc ttgggaaaac tctcaatgtt 3300
cctccgcccc cgaaccccag tagggccagc acttccgaga cacccattaa aaagggcaca 3360
gacgcgagat tggcctcatt tggaacggag atcgcgtctt tattgacagg tggtgcaacc 3420
caatgtgctc gaaagtcacc ctcggaacca tcagggccag gtgcacctgc ggggaatgtc 3480
cccgagtgtg tgagcaatgc cgcactgata caggagtgga cacccgaatc tggtaccaca 3540
atctccccga gatcccagaa taatgaagaa gggggagact attatgatga tgagctgttc 3600
tccgatgtcc aagacatcaa aacagccttg gccaaaatac acgaggataa tcagaagata 3660
atctccaagc tagaatcatt gctgttattg aagggagaag ttgagtcaat taagaagcag 3720
atcaacaggc aaaatatcag catatccacc ctggaaggac acctctcaag catcatgatt 3780
gccattcctg gacttgggaa ggatcccaac gaccccactg cagatgtcga actcaatccc 3840
gacctgaaac ccatcatagg cagagattca ggccgagcac tggccgaagt tctcaagaag 3900
cccgttgcca gccgacaact ccagggaatg actaatggac ggaccagttc cagaggacag 3960
ctgctgaagg aatttcaact aaagccgatc gggaaaaagg tgagctcagc cgtcgggttt 4020
gttcctgaca ccggccctgc atcacgcagt gtaatccgct ccattataaa atccagccgg 4080
ctagaggagg atcggaagcg ttacctgatg actctccttg atgatatcaa aggagccaac 4140
gatcttgcca agttccacca gatgctgatg aagataataa tgaagtagct acagctcaac 4200
ttacctgcca accccatgcc agtcgaccta attagtacaa cctaaatcca ttataaaaaa 4260
cttaggagca aagtgattgc ctcctaagtt ccacaatgac agagatctac gatttcgaca 4320
agtcggcatg ggacatcaaa gggtcgatcg ctccgataca acctaccacc tacagtgatg 4380
gcaggctggt gccccaggtc agagtcatag atcctggtct aggtgatagg aaggatgaat 4440
gctttatgta catgtttctg ctgggggttg ttgaggacag cgatccccta gggcctccaa 4500
tcgggcgagc attcgggtcc ctgcccttag gtgttggtag atccacagca aaacccgagg 4560
aactcctcaa agaggccact gagcttgaca tagttgttag acgtacagca gggctcaatg 4620
aaaaactggt gttctacaac aacaccccac taaccctcct cacaccttgg agaaaggtcc 4680
taacaacagg gagtgtcttc aatgcaaacc aagtgtgcaa tgcggttaat ctaataccgc 4740
tggacacccc gcagaggttc cgtgttgttt atatgagcat cacccgtctt tcggataacg 4800
ggtattacac cgttcccaga agaatgctgg aattcagatc ggtcaatgca gtggccttca 4860
acctgctagt gacccttagg attgacaagg cgattggccc tgggaagatc atcgacaatg 4920
cagagcaact tcctgaggca acatttatgg tccacatcgg gaacttcagg agaaagaaga 4980
gtgaagtcta ctctgccgat tattgcaaaa tgaaaatcga aaagatgggc ctggtttttg 5040
cacttggtgg gatagggggc accagtcttc acattagaag cacaggcaaa atgagcaaga 5100
ctctccatgc acaactcggg ttcaagaaga ccttatgtta cccactgatg gatatcaatg 5160
aagaccttaa tcggttactc tggaggagca gatgcaagat agtaagaatc caggcagttt 5220
tgcagccatc agttcctcaa gaattccgca tttacgacga cgtgatcata aatgatgacc 5280
aaggactatt caaagttctg tagaccgcag tgcccagcaa tacccgaaaa cgacccccct 5340
cataatgaca gccagaaggc ccggacaaaa aagccccctc cagaagactc cacggaccaa 5400
gcgagaggcc agccagcagc cgacagcaag tgtggacacc aggcggccca agcacagaac 5460
agccccgaca caaggccacc accagccatc ccaatctgcg tcctcctcgt gggacccccg 5520
aggaccaacc ccgaaggtcg ctccgaacac agaccaccaa ccgcatcccc acagctcccg 5580
ggaaaggaac ccccagcaac tggaaggccc ctccccccct cccccaacgc aagaacccca 5640
caaccgaacc gcacaagcga ccgaggtgac ccaaccgcag gcatccgact ccttagacag 5700
atcctctccc cccggcatac taaacaaaac ttagggccaa ggaacacaca cactcgacag 5760
aacccagacc ccggcccgcg gcaccgcgcc cccacccccc gaaaaccaga gggagccccc 5820
aaccaaaccc gccggccccc ccggtgccca caggtaggca caccaacccc cgaccagacc 5880
cagcacccag ccaccgacaa tccaagacgg ggggcccccc ccaaaaaaag gcccccaggg 5940
gccgacagcc agcatcgcga ggaagcacac ccaccccaca cacgaccacg gcaaccgaac 6000
cagagtccag accaccctgg gccaccagct cccagactcg gccatcaccc cgcaaaaagg 6060
aaaggccaca acccgcgcac cccagccccg atccggcggg cagccactca acccgaacca 6120
gcacccaaga gcgatccctg ggggaccccc aaaccgcaaa agacatcagt atcccacagc 6180
ctctccaagt cccccggtct cctcctcttc tcgaagggac caaaagatca atccaccaca 6240
tccgacgaca ctcaattccc cacccccaaa ggagacaccg ggaatcccag aatcaagact 6300
catccagtgt ccatcatggg tctcaaggtg aacgtctctg ccatattcat ggcagtactg 6360
ttaactctcc aaacacccac cggtcaaatc cattggggca atctctctaa gataggggtg 6420
gtagggatag gaagtgcaag ctacaaagtt atgactcgtt ccagccatca atcattggtc 6480
ataaaattaa tgcccaatat aactctcctc aataactgca cgagggtaga gatcgcagaa 6540
tacaggagac tactgagaac agttttggaa ccaattagag atgcacttaa tgcaatgacc 6600
cagaatataa gaccggttca gagtgtagct tcaagtagga gacacaagag atttgcggga 6660
gttgtcctgg caggtgcggc cctaggcgtt gccacagctg ctcagataac agccggcatt 6720
gcacttcacc agtccatgct gaactctcaa gccatcgaca atctgagagc aagcctggaa 6780
actactaatc aggcaattga ggcaatcaga caagcagggc aggagatgat attggctgtt 6840
cagggtgtcc aagactacat caataatgag ctgataccgt ctatgaacca actatcttgt 6900
gatttaatcg gccagaagct agggctcaaa ttgctcagat actatacaga aatcctgtca 6960
ttatttggcc ccagcttacg ggaccccata tctgcggaga tatccatcca ggctttgagc 7020
tatgcgcttg gaggagatat caataaggta ttagaaaagc tcggatacag tggaggtgat 7080
ttactgggca tcttagagag cagaggaata aaggcccgga taactcacgt cgacacagag 7140
tcctacttca ttgtactcag tatagcctat ccgacgctgt ccgagattaa gggggtgatt 7200
gtccaccggc tagagggggt ctcgtacaat ataggctctc aagagtggta taccactgtg 7260
cccaagtatg ttgcaaccca agggtacctt atctcgaatt ttgatgagtc atcgtgtact 7320
ttcatgccag aggggactgt gtgcagccaa aatgccttgt acccgatgag tcctctgctc 7380
caagaatgcc tccgggggtc caccaagtcc tgtgctcgta cactcgtatc cgggtctttt 7440
gggaaccggt tcattttatc acaagggaac ctaatagcca attgtgcatc aatcctctgc 7500
aagtgttaca caacaggaac gatcattaat caagaccctg acaagatcct aacatacatt 7560
gctgccgatc actgcccggt ggtcgaggtg aacggtgtga ccatccaagt cgggagcagg 7620
aggtatccgg acgcggtgta cctgcacaga attgacctcg gtcctcccat atcattggag 7680
aggttggacg tagggacaaa tctggggaat gcaattgcta agttggagga tgccaaggaa 7740
ttgttggagt catcggacca gatattgagg agtatgaaag gtttatcgag cactagcata 7800
gtttacatcc tgattgcagt gtgtcttgga gggttgatag ggatccccgc tttaatatgt 7860
tgctgcaggg ggcgctgtaa caaaaaggga gaacaagttg gtatgtcaag accaggccta 7920
aagcctgatc ttacagggac atcaaaatcc tatgtaaggt cgctctgatc ctctacaact 7980
cttgaaacac agatttccca caagtctcct cttcgtcatc aagcaaccac cgcatccagc 8040
atcaagccca cctgaaattg tctccggctt ccctctggcc gaacgatatc ggtagttaat 8100
taaaacttag ggtgcaagat catccacaat gtcaccacaa cgagaccgaa taaatgcctt 8160
ctacaaagac aacccacatc ctaagggaag taggatagtt attaacagag aacatcttat 8220
gattgataga ccttatgttt tgctggctgt tctattcgtc atgtttctga gcttgatcgg 8280
gttgctagcc attgcaggca ttagactcca tcgtgcagcc atctacaccg cagagatcca 8340
taagagcctc agcaccaatc tagatgtaac taactcgatc gagcatcagg tcaaggacgt 8400
gctgacacca ctcttcaaga tcattggtga tgaagtgggc ctgaggacac ctcagagatt 8460
cactgaccta gtgaaattca tctctgacaa aattaaattc cttaatccgg atagggagta 8520
cgacttcaga gatctcactt ggtgtatcaa cccgccagag agaatcaaat tggattatga 8580
tcaatactgt gcagatgtgg ctgctgaaga actcatgaat gcattggtga actcaactct 8640
actggaggcc agggcaacca atcagttcct agctgtctca aagggaaact gctcagggcc 8700
cactacaatc agaggtcaat tctcaaacat gtcgctgtcc ctgttggact tgtatttaag 8760
tcgaggttac aatgtgtcat ctatagtcac tatgacatcc cagggaatgt acgggggaac 8820
ttacctagtg ggaaagccta atctgagcag taaagggtca gagttgtcac aactgagcat 8880
gcaccgagtg tttgaagtag gggttatcag aaatccgggt ttgggggctc cggtgttcca 8940
tatgacaaac tattttgagc aaccagtcag taatgatttc agcaactgca tggtggcttt 9000
gggggagctt aaattcgcag ccctctgtca cagggaagat tctatcacaa ttccctatca 9060
ggggtcaggg aaaggtgtca gcttccagct cgtcaagcta ggtgtctgga aatccccaac 9120
cgacatgcga tcctgggtcc ccctatcaac ggatgatcca gtgatagata ggctttatct 9180
ctcatctcac agaggtgtta tcgctgacaa tcaagcaaaa tgggctgtcc cgacaacacg 9240
gacagatgac aagttgcgaa tggagacatg cttccagcag gcgtgtaagg gtaaaaacca 9300
agcactctgc gagaatcccg agtgggcacc attgaaggat aacaggattc cttcatacgg 9360
ggtcttgtct gttaatctga gtctgacagt tgagcttaaa atcaaaattg cttcaggatt 9420
cgggccattg atcacacacg gttcagggat ggacctatac aaaaccaacc acaacaatgt 9480
gtattggctg actatcccgc caatgaagaa cctagcctta ggtgtaatca acacattgga 9540
gtggataccg agattcaagg ttagtcccaa cctcttcact gttccaatca aggaagcagg 9600
cgaggactgc catgccccaa catacctacc tgcggaggtg gatggtgatg tcaaactcag 9660
ttccaatctg gtaattctac ctggtcagga tctccaatat gttttggcaa cctacgatac 9720
ttccagggtt gaacatgctg tggtttatta tgtttacagc ccaagccgct cattttctta 9780
cttttatcct tttaggttgc ctataaaggg ggtcccaatc gaattacaag tggaatgctt 9840
cacatgggac aaaaaactct ggtgccgtca cttctgtgtg cttgcggact cagaatctgg 9900
tggacatatc actcactctg ggatggtggg catgggagtc agctgcacag tcactcggga 9960
agatggaacc aatcgcagat agggctgcca gtgaaccgat cacatgatgt cacccagaca 10020
tcaggcatac ccactagtgt gaaatagaca tcagaattaa gaaaaacgta gggtccaagt 10080
ggtttcccgt tatggactcg ctatctgtca accagatctt ataccctgaa gttcacctag 10140
atagcccgat agttaccaat aagatagtag ctatcctgga gtatgctcga gtccctcacg 10200
cttacagcct ggaggaccct acactgtgtc agaacatcaa gcaccgccta aaaaacggat 10260
tctccaacca aatgattata aacaatgtgg aagttgggaa tgtcatcaag tccaagctta 10320
ggagttatcc ggcccactct catattccat atccaaattg taatcaggat ttatttaaca 10380
tagaagacaa agagtcaaca aggaagatcc gtgagctcct aaaaaaggga aattcgctgt 10440
actccaaagt cagtgataag gttttccaat gcctgaggga cactaactca cggcttggcc 10500
taggctccga attgagggag gacatcaagg agaaaattat taacttggga gtttacatgc 10560
acagctccca atggtttgag ccctttctgt tttggtttac agtcaagact gagatgaggt 10620
cagtgattaa atcacaaacc catacttgcc ataggaggag acacacacct gtattcttca 10680
ctggtagttc agttgagctg ttaatctctc gtgaccttgt tgctataatc agtaaggaat 10740
ctcaacatgt atattacctg acgtttgaac tggttttgat gtattgtgat gtcatagagg 10800
ggaggttaat gacagagacc gctatgacca ttgatgctag gtatgcagaa cttctaggaa 10860
gagtcagata catgtggaaa ctgatagatg gtttcttccc tgcactcggg aatccaactt 10920
atcaaattgt agccatgctg gagccacttt cacttgctta cctgcaactg agggatataa 10980
cagtagaact cagaggtgct ttccttaacc actgctttac tgaaatacat gatgttcttg 11040
accaaaacgg gttttctgat gaaggtactt atcatgagtt aattgaagcc ctagattaca 11100
ttttcataac tgatgacata catctgacag gggagatttt ctcatttttc agaagtttcg 11160
gccaccccag acttgaagca gtaacggctg ctgaaaatgt caggaaatac atgaatcagc 11220
ctaaagtcat tgtgtatgag actctgatga aaggtcatgc catattttgt ggaatcataa 11280
tcaacggcta tcgtgacagg cacggaggca gttggccacc cctgaccctc cccctgcatg 11340
ctgcagacac aatccggaat gctcaagctt caggtgaagg gttaacacat gagcagtgcg 11400
ttgataactg gaaatcattt gctggagtga gatttggctg ttttatgcct cttagcctgg 11460
acagtgatct gacaatgtac ctaaaggaca aggcacttgc tgctctccaa agggaatggg 11520
attcagttta cccgaaagag ttcctgcgtt acgatcctcc caagggaacc gggtcacgga 11580
ggcttgtaga tgttttcctt aatgattcga gctttgaccc atatgatatg ataatgtatg 11640
tcgtaagtgg agcctacctc catgaccctg agttcaacct gtcttacagc ctgaaagaaa 11700
aggagatcaa ggaaacaggt agacttttcg ctaaaatgac ttacaaaatg agggcatgcc 11760
aagtgatcgc tgaaaatcta atctcaaacg ggattggcaa gtattttaag gacaatggga 11820
tggccaagga tgagcacgat ttgactaagg cactccacac tctggctgtc tcaggagtcc 11880
ccaaagatct caaagaaagt cacagggggg ggccagtctt aaaaacctac tcccgaagcc 11940
cagtccacac aagtaccagg aacgttaaag cagaaaaagg gtttgtagga ttccctcatg 12000
taattcggca gaatcaagac actgatcatc cggagaatat agaaacctac gagacagtca 12060
gcgcatttat cacgactgat ctcaagaagt actgccttaa ttggagatat gagaccatca 12120
gcttatttgc acagaggcta aatgagattt acggattacc ctcatttttt cagtggctgc 12180
ataagaggct tgaaacctct gtcctctatg taagtgaccc tcattgcccc cccgaccttg 12240
acgcccatgt cccgttatgc aaagtcccca atgaccaaat cttcatcaag taccctatgg 12300
gaggtataga agggtattgt cagaagctgt ggaccatcag caccattccc tacttatacc 12360
tggctgctta tgagagcggg gtaaggattg cttcgttagt gcaaggggac aatcagacca 12420
tagccgtaac aaaaagggta cccagcacat ggccttacaa ccttaagaaa cgggaagctg 12480
ctagagtaac tagagattac tttgtaattc ttaggcaaag gctacatgac attggccatc 12540
acctcaaggc aaatgagaca attgtttcat cacatttttt tgtctattca aaaggaatat 12600
attatgatgg gctacttgtg tcccaatcac tcaagagcat cgcaagatgt gtattctggt 12660
cagagactat agttgatgaa acaagggcgg catgcagtaa tattgctaca acaatggcca 12720
aaagcattga gagaggttat gaccgttatc ttgcatattc cctgaacgtc ctaaaagtga 12780
tacagcaaat tttgatctcc cttggcttca caatcaattc aaccatgacc cgggatgtag 12840
tcatacccct cctcacaaac aacgatctct taataaggat ggcactgttg cccgctccta 12900
ttgggggtat gaattatctg aatatgagca ggctgtttgt cagaaacatc ggtgatccag 12960
taacatcatc aattgctgat ctcaagagaa tgattctcgc atcactaatg cctgaagaga 13020
ccctccatca agtaatgaca caacaaccgg gggactcttc attcctagac tgggctagcg 13080
acccttactc agcaaatctt gtatgcgtcc agagcatcac tagactcctc aagaacataa 13140
ctgcaaggtt tgtcctgatc cacagtccaa acccaatgtt aaaagggtta ttccatgatg 13200
acagcaaaga agaggacgag agactggcgg cattcctcat ggacaggcat attatagtac 13260
ctagggcagc tcatgaaatc ctggatcata gtgtcacagg ggcaagagag tctattgcag 13320
gcatgctaga taccacaaaa ggcctgattc gagccagcat gaggaagggg gggttaacct 13380
ctcgagtgat aaccagattg tccaattatg actatgaaca atttagagca gggatggtgc 13440
tattgacagg aagaaagaga aatgtcctca ttgacaaaga gtcatgttca gtgcagctgg 13500
ctagagccct aagaagccat atgtgggcaa gactagctcg aggacggcct atttacggcc 13560
ttgaggtccc tgatgtacta gaatctatgc gaggccacct tattcggcgt catgagacat 13620
gtgtcatctg cgagtgtgga tcagtcaact acggatggtt ttttgtcccc tcgggttgcc 13680
aactggatga tattgacaag gaaacatcat ccttgagagt cccatatatt ggttctacca 13740
ctgatgagag aacagacatg aagctcgcct tcgtaagagc cccaagtaga tccttgcgat 13800
ctgccgttag aatagcaaca gtgtactcat gggcttacgg tgatgatgat agctcttgga 13860
acgaagcctg gttgttggca aggcaaaggg ccaatgtgag cctggaggag ctaagggtga 13920
tcactcccat ctcgacttcg actaatttag cgcataggtt gagggatcgt agcactcaag 13980
tgaaatactc aggtacatcc cttgtccgag tggcaaggta taccacaatc tccaacgaca 14040
atctctcatt tgtcatatca gataagaagg ttgatactaa ctttatatac caacaaggaa 14100
tgcttctagg gttgggtgtt ttagaaacat tgtttcgact cgagaaagat actggatcat 14160
ctaacacggt attacatctt cacgtcgaaa cagattgttg cgtgatcccg atgatagatc 14220
atcccaggat acccagctcc cgcaagctag agctgagggc agagctatgt accaacccat 14280
tgatatatga taatgcacct ttaattgaca gagatgcaac aaggctatac acccagagcc 14340
ataggaggca ccttgtggaa tttgttacat ggtccacacc ccaactatat cacattctag 14400
ctaagtccac agcactatct atgattgacc tggtaacaaa atttgagaag gaccatatga 14460
atgaaatttc agctctcata ggggatgacg atatcaatag tttcataact gagtttctgc 14520
ttatagagcc aagattattc accatctact tgggccagtg tgcagccatc aattgggcat 14580
ttgatgtaca ttatcataga ccatcaggga aatatcagat gggtgagctg ttgtcttcgt 14640
tcctttctag aatgagcaaa ggagtgttta aggtgcttgt caatgctcta agccacccaa 14700
agatctacaa gaaattctgg cattgtggta ttatagagcc tatccatggt ccttcacttg 14760
atgctcaaaa cttgcacaca actgtgtgca acatggttta cacatgctat atgacctacc 14820
tcgacctgtt gttgaatgaa gagttagaag agttcacatt tcttttgtgt gaaagcgatg 14880
aggatgtagt accggacaga ttcgacaaca tccaggcaaa acacttgtgt gttctggcag 14940
atttgtactg tcaaccaggg acctgcccac cgattcgagg tctaaggccg gtagagaaat 15000
gtgcagttct aaccgatcat atcaaggcag aggctaggtt atctccagca ggatcttcgt 15060
ggaacataaa tccaattatt gtagaccatt actcatgctc tctgacttat ctccgtcgag 15120
gatctatcaa acagataaga ttgagagttg atccaggatt catttttgac gccctcgctg 15180
aggtaaatgt cagtcagcca aaggtcggca gcgacgtcaa gatggacaaa aagactatag 15240
tttggtttag aagagaccta aggattgagg ataatcctgc attagcagca gctgctcacg 15300
aaggatctgt ttttcctgtc ttcatttggt gtcctgaaga agaaggacag ttttatcctg 15360
gaagagcttc aagatggtgg atgaaacaat cacttgctca cttatctcaa tccttgaagg 15420
ctcttggatc tgacctcact ttaatcaaaa cccacaacac gatttcagcg atcttggatt 15480
gtatccgcgt taccggtgct acaaaagtcg tctttaacca cctctatgat cctgtttcgt 15540
tagttcggga ccataccgta aaggagaagc tggtggaacg tgggatctct gtgcaaagct 15600
acaatggaga tctattgtat gaaccgtggg agatatactg cgaaaagggc aaacctttta 15660
cgagtttcaa ttcttactgg aagaaatgct tagatatgtc gattgaatcc gttatgcttc 15720
ctcctccttg gcggttgatg ccaataactg cagcggctga agcgatttgg gcgtgttcga 15780
ttgaagaact agggctggag aatgaggccg agaaaccgag caatgcgttg ttaactagag 15840
cttggtctcc aggatggagc aatgctgata agttactaaa tgagttcatc gagaagcagt 15900
tgatagatta tgcaaagaac agcaagaaag ttgttgggaa ttctacttca ctactttctc 15960
cgtatctcca tttcggggaa ataagcgtca gacacgtttt ccagtgtgcc cggatgaaac 16020
aaattatatg ggcaagagat aagaacagtg aaggagaaga aagtgcagat ctttttctta 16080
ggggaatcgg tttaagagag tattctcggt atatatgttt caacttcccg tttactcacg 16140
agcaatcgtt gttgagtcat cttcggtttt tcccttggga tgctgatgtt gataagttca 16200
aggcctggag acaaggcagg accggttatc cgttggtgga tgccggaatg agagagcttt 16260
gggctaccgg atggatgcat aacagaataa gagtgattgt ttcaagcttt gctgtgaagt 16320
ttcttctcct tccatggaaa tggggaatga agtatttctg ggatacactt ttggatgctg 16380
atttggaatg tgacatcctt ggctggcagt atatctctgg gagtatcccc gatggccacg 16440
agcttgatcg cttggacaat cccgcgttac aaggcgccaa atatgaccca gaaggtgagt 16500
acataaggca atggcttccc gagcttgcga gattgccaac tgaatggatc catcatccat 16560
gggacgctcc tttaaccgta ctcaaagctt ctggtgtgga actcggaaca aactatgcga 16620
aacccattgt agacatcgac acagctcgtg agctactagc taaagctatt tcaagaaccc 16680
gtgaagcaca gatcatgatc ggagcagcac ctgatgagat tgtagcagat agcttcgagg 16740
ccttaggggc taataccatt aaagaacctg gtctttgccc atctgtgtct tctaatgacc 16800
aacaagtacc ttcggctgtt cgttacaacg ggtcagcggc agtgaaacct gaggaagaag 16860
aagagagaga catgaagaaa tctaggggat tcgatgaaag ggagttgttt tcgactgctg 16920
aatcttcttc ttcttcgagt gtgtttttcg tttcgcagtc ttgctcgttg gcatcagaag 16980
ggaagaatct ggaaggtatt caagattcat ctgatcagat tactacaagt ttgggaaaaa 17040
atggttgcaa aaccggtaat tccgctgacg gcggcggagg atcgggtggt agtggtggtt 17100
caggaggagg atcgacccaa ggaggatcca tgaatggagc tataggaggt gaccttttgc 17160
tcaattttcc tgacatgtcg gtcctagagc gccaaagggc tcacctcaag tacctcaatc 17220
ccacctttga ttctcctctc gccggcttct ttgccgattc ttcaatgatt accggcggcg 17280
agatggacag ctatctttcg actgccggtt tgaatcttcc gatgatgtac ggtgagacga 17340
cggtggaagg tgattcaaga ctctcaattt cgccggaaac gacgcttggg actggaaatt 17400
tcaaggcagc gaagtttgat acagagacta aggattgtaa tgaggcggcg aagaagatga 17460
cgatgaacag agatgaccta gtagaagaag gagaagaaga gaagtcgaaa ataacagagc 17520
aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca caaagccaag aaagaagaga 17580
acaatttctc taatgattca tctaaagtga cgaaggaatt ggagaaaacg gattatattg 17640
gctatccgta cgacgtccca gattacgcgg gcatctcaaa tatgagcatc aaggatttca 17700
gacctccaca cgatgatgtt gcaaaattgc tcaaagatat caacacaagc aagcacaatc 17760
ttcccatttc agggggtagt ctcgccaatt atgaaatcca tgctttccgc agaatcgggt 17820
taaactcatc tgcttgctac aaagctgttg agatatcaac attaattagg agatgccttg 17880
agccagggga agacggcttg ttcttgggtg aggggtcggg ttctatgttg atcacttata 17940
aggagatact aaaactaaac aagtgcttct ataatagtgg ggtttccgcc aattctagat 18000
ctggtcaaag ggaattagca ccctatccct ccgaagttgg ccttgtcgaa cacagaatgg 18060
gagtaggtaa tattgtcaag gtgctcttta acgggaggcc cgaagtcacg tgggtaggca 18120
gtatagattg cttcaatttc atagtcagta atatccctac ctctagtgtg gggtttatcc 18180
attcagatat agagacctta cctaacaaag atactataga gaagctagag gaattggcag 18240
ccatcttatc gatggctcta ctccttggca aaataggatc aatactggtg attaagctta 18300
tgcctttcag cggggatttt gttcagggat ttataagcta tgtagggtct cattatagag 18360
aagtgaacct tgtctaccct aggtacagca acttcatatc tactgaatct tatttagtta 18420
tgacagatct caaagctaac cggctaatga atcctgaaaa gatcaagcag cagataattg 18480
aatcatctgt acggacttca cctggactta taggtcacat cctatccatt aagcaactaa 18540
gctgcataca agcaattgtg ggaggcgcgg ttagtagagg tgatatcaac cctattctga 18600
aaaaacttac acctatagag caggtgctga tcagttgcgg gttggcaatt aacggaccta 18660
aactgtgcaa agaattaatc caccatgatg ttgcctcagg gcaagatgga ttgcttaact 18720
ctatactcat cctctacagg gagttggcaa gattcaaaga caaccaaaga agtcaacaag 18780
ggatgttcca cgcttacccc gtattggtaa gtagtaggca acgagaactt gtatctagga 18840
tcactcgcaa attttggggg catattcttc tttactccgg gaacagaaag ttgataaatc 18900
ggtttatcca gaatctcaag tccggttatc tagtactaga cttacaccag aatatcttcg 18960
ttaagaatct atccaagtca gagaaacaga ttattatgac ggggggttta aaacgtgagt 19020
gggtttttaa ggtaacagtc aaggagacca aagaatggta caagttagtc ggatacagcg 19080
ctctgattaa ggattaattg gttgaactcc ggaaccctaa tcctgcccta ggtagttagg 19140
cattatttgc aatatattaa agaaaacttt gaaaatacga agtttctatt cccagctttg 19200
tctggtggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac attccgaggg 19260
gaccgtcccc tcggtaatgg cgaatgggac gcggccgccg cgctcactgg ccgtcgtttt 19320
acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 19380
ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 19440
gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 19500
ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 19560
tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 19620
gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 19680
gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 19740
ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 19800
ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 19860
tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 19920
ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 19980
tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 20040
aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 20100
tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 20160
ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 20220
tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 20280
gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 20340
aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 20400
agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 20460
acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 20520
actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 20580
accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 20640
actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 20700
cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 20760
cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 20820
gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 20880
ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 20940
tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 21000
aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 21060
gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 21120
acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 21180
tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 21240
ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 21300
atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 21360
agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 21420
cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 21480
agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 21540
acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 21600
gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 21660
ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 21720
gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 21780
gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 21840
gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 21900
tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 21960
gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 22020
ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 22080
gccaagcgcg ccagtgaatt gtaatacgac tcactata 22118
<210> 16
<211> 21776
<212> DNA
<213> Artificial Sequence
<220>
<223> p(+)MV323-CRYPHR-CIB1N
<400> 16
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
acgagatggc cacacttttg aggagcttag cattgttcaa aagaaacaag gacaaaccac 1020
ccattacatc aggatccggt ggagccatca gaggaatcaa acacattatt atagtaccaa 1080
ttcctggaga ttcctcaatt accactcgat ccagactact ggaccggttg gtcaggttaa 1140
ttggaaaccc ggatgtgagc gggcccaaac taacaggggc actaataggt atattatcct 1200
tatttgtgga gtctccaggt caattgattc agaggatcac cgatgaccct gacgttagca 1260
tcaggctgtt agaggttgtt cagagtgacc agtcacaatc tggccttacc ttcgcatcaa 1320
gaggtaccaa catggaggat gaggcggacc aatacttttc acatgatgat ccaagcagta 1380
gtgatcaatc caggtccgga tggttcgaga acaaggaaat ctcagatatt gaagtgcaag 1440
accctgaggg attcaacatg attctgggta ccattctagc ccagatctgg gtcttgctcg 1500
caaaggcggt tacggcccca gacacggcag ctgattcgga gctaagaagg tggataaagt 1560
acacccaaca aagaagggta gttggtgaat ttagattgga gagaaaatgg ttggatgtgg 1620
tgaggaacag gattgccgag gacctctctt tacgccgatt catggtggct ctaatcctgg 1680
atatcaagag gacacccggg aacaaaccta ggattgctga aatgatatgt gacattgata 1740
catatatcgt agaggcagga ttagccagtt ttatcctgac tattaagttt gggatagaaa 1800
ctatgtatcc tgctcttgga ctgcatgaat ttgctggtga gttatccaca cttgagtcct 1860
tgatgaatct ttaccagcaa atgggagaaa ctgcacccta catggtaatc ctagagaact 1920
caattcagaa caagttcagt gcaggatcat accctctgct ctggagctat gccatgggag 1980
taggagtgga acttgaaaac tccatgggag gtttgaactt tggtcgatct tactttgatc 2040
cagcatattt tagattaggg caagagatgg tgaggaggtc agctggaaag gtcagttcca 2100
cattggcatc cgaactcggt atcactgccg aggatgcaag gcttgtttca gagattgcaa 2160
tgcatactac tgaggacagg atcagtagag cggtcggacc cagacaagcc caagtgtcat 2220
ttctacacgg tgatcaaagt gagaatgagc taccaggatt ggggggcaag gaagatagga 2280
gggtcaaaca gggtcgggga gaagccaggg agagctacag agaaaccggg tccagcagag 2340
caagtgatgc gagagctgcc catcctccaa ccagcatgcc cctagacatt gacactgcat 2400
cggagtcagg ccaagatccg caggacagtc gaaggtcagc tgacgccctg ctcaggctgc 2460
aagccatggc aggaatcttg gaagaacaag gctcagacac ggacacccct agggtataca 2520
atgacagaga tcttctagac taggtgcgag aggccgagga ccagaacaac atccgcctac 2580
cctccatcat tgttataaaa aacttaggaa ccaggtccac acagccgcca gccaaccaac 2640
catccactcc cacgactgga gccgatggca gaagagcagg cacgccatgt caaaaacgga 2700
ctggaatgca tccgggctct caaggccgag cccatcggct cactggccgt cgaggaagcc 2760
atggcagcat ggtcagaaat atcagacaac ccaggacagg accgagccac ctgcaaggaa 2820
gaggaggcag gcagttcggg tctcagcaaa ccatgcctct cagcaattgg atcaactgaa 2880
ggcggtgcac ctcgcatccg cggtcaggga tctggagaaa gcgatgacga cgctgaaact 2940
ttgggaatcc cctcaagaaa tctccaggca tcaagcactg ggttacagtg ttatcatgtt 3000
tatgatcaca gcggtgaagc ggttaaggga atccaagatg ctgactctat catggttcaa 3060
tcaggccttg atggtgatag caccctctca ggaggagacg atgaatctga aaacagcgat 3120
gtggatattg gcgaacctga taccgaggga tatgctatca ctgaccgggg atctgctccc 3180
atctctatgg ggttcagggc ttctgatgtt gaaactgcag aaggagggga gatccacgag 3240
ctcctgaaac tccaatccag aggcaacaac tttccgaagc ttgggaaaac tctcaatgtt 3300
cctccgcccc cgaaccccag tagggccagc acttccgaga cacccattaa aaagggcaca 3360
gacgcgagat tggcctcatt tggaacggag atcgcgtctt tattgacagg tggtgcaacc 3420
caatgtgctc gaaagtcacc ctcggaacca tcagggccag gtgcacctgc ggggaatgtc 3480
cccgagtgtg tgagcaatgc cgcactgata caggagtgga cacccgaatc tggtaccaca 3540
atctccccga gatcccagaa taatgaagaa gggggagact attatgatga tgagctgttc 3600
tccgatgtcc aagacatcaa aacagccttg gccaaaatac acgaggataa tcagaagata 3660
atctccaagc tagaatcatt gctgttattg aagggagaag ttgagtcaat taagaagcag 3720
atcaacaggc aaaatatcag catatccacc ctggaaggac acctctcaag catcatgatt 3780
gccattcctg gacttgggaa ggatcccaac gaccccactg cagatgtcga actcaatccc 3840
gacctgaaac ccatcatagg cagagattca ggccgagcac tggccgaagt tctcaagaag 3900
cccgttgcca gccgacaact ccagggaatg actaatggac ggaccagttc cagaggacag 3960
ctgctgaagg aatttcaact aaagccgatc gggaaaaagg tgagctcagc cgtcgggttt 4020
gttcctgaca ccggccctgc atcacgcagt gtaatccgct ccattataaa atccagccgg 4080
ctagaggagg atcggaagcg ttacctgatg actctccttg atgatatcaa aggagccaac 4140
gatcttgcca agttccacca gatgctgatg aagataataa tgaagtagct acagctcaac 4200
ttacctgcca accccatgcc agtcgaccta attagtacaa cctaaatcca ttataaaaaa 4260
cttaggagca aagtgattgc ctcctaagtt ccacaatgac agagatctac gatttcgaca 4320
agtcggcatg ggacatcaaa gggtcgatcg ctccgataca acctaccacc tacagtgatg 4380
gcaggctggt gccccaggtc agagtcatag atcctggtct aggtgatagg aaggatgaat 4440
gctttatgta catgtttctg ctgggggttg ttgaggacag cgatccccta gggcctccaa 4500
tcgggcgagc attcgggtcc ctgcccttag gtgttggtag atccacagca aaacccgagg 4560
aactcctcaa agaggccact gagcttgaca tagttgttag acgtacagca gggctcaatg 4620
aaaaactggt gttctacaac aacaccccac taaccctcct cacaccttgg agaaaggtcc 4680
taacaacagg gagtgtcttc aatgcaaacc aagtgtgcaa tgcggttaat ctaataccgc 4740
tggacacccc gcagaggttc cgtgttgttt atatgagcat cacccgtctt tcggataacg 4800
ggtattacac cgttcccaga agaatgctgg aattcagatc ggtcaatgca gtggccttca 4860
acctgctagt gacccttagg attgacaagg cgattggccc tgggaagatc atcgacaatg 4920
cagagcaact tcctgaggca acatttatgg tccacatcgg gaacttcagg agaaagaaga 4980
gtgaagtcta ctctgccgat tattgcaaaa tgaaaatcga aaagatgggc ctggtttttg 5040
cacttggtgg gatagggggc accagtcttc acattagaag cacaggcaaa atgagcaaga 5100
ctctccatgc acaactcggg ttcaagaaga ccttatgtta cccactgatg gatatcaatg 5160
aagaccttaa tcggttactc tggaggagca gatgcaagat agtaagaatc caggcagttt 5220
tgcagccatc agttcctcaa gaattccgca tttacgacga cgtgatcata aatgatgacc 5280
aaggactatt caaagttctg tagaccgcag tgcccagcaa tacccgaaaa cgacccccct 5340
cataatgaca gccagaaggc ccggacaaaa aagccccctc cagaagactc cacggaccaa 5400
gcgagaggcc agccagcagc cgacagcaag tgtggacacc aggcggccca agcacagaac 5460
agccccgaca caaggccacc accagccatc ccaatctgcg tcctcctcgt gggacccccg 5520
aggaccaacc ccgaaggtcg ctccgaacac agaccaccaa ccgcatcccc acagctcccg 5580
ggaaaggaac ccccagcaac tggaaggccc ctccccccct cccccaacgc aagaacccca 5640
caaccgaacc gcacaagcga ccgaggtgac ccaaccgcag gcatccgact ccttagacag 5700
atcctctccc cccggcatac taaacaaaac ttagggccaa ggaacacaca cactcgacag 5760
aacccagacc ccggcccgcg gcaccgcgcc cccacccccc gaaaaccaga gggagccccc 5820
aaccaaaccc gccggccccc ccggtgccca caggtaggca caccaacccc cgaccagacc 5880
cagcacccag ccaccgacaa tccaagacgg ggggcccccc ccaaaaaaag gcccccaggg 5940
gccgacagcc agcatcgcga ggaagcacac ccaccccaca cacgaccacg gcaaccgaac 6000
cagagtccag accaccctgg gccaccagct cccagactcg gccatcaccc cgcaaaaagg 6060
aaaggccaca acccgcgcac cccagccccg atccggcggg cagccactca acccgaacca 6120
gcacccaaga gcgatccctg ggggaccccc aaaccgcaaa agacatcagt atcccacagc 6180
ctctccaagt cccccggtct cctcctcttc tcgaagggac caaaagatca atccaccaca 6240
tccgacgaca ctcaattccc cacccccaaa ggagacaccg ggaatcccag aatcaagact 6300
catccagtgt ccatcatggg tctcaaggtg aacgtctctg ccatattcat ggcagtactg 6360
ttaactctcc aaacacccac cggtcaaatc cattggggca atctctctaa gataggggtg 6420
gtagggatag gaagtgcaag ctacaaagtt atgactcgtt ccagccatca atcattggtc 6480
ataaaattaa tgcccaatat aactctcctc aataactgca cgagggtaga gatcgcagaa 6540
tacaggagac tactgagaac agttttggaa ccaattagag atgcacttaa tgcaatgacc 6600
cagaatataa gaccggttca gagtgtagct tcaagtagga gacacaagag atttgcggga 6660
gttgtcctgg caggtgcggc cctaggcgtt gccacagctg ctcagataac agccggcatt 6720
gcacttcacc agtccatgct gaactctcaa gccatcgaca atctgagagc aagcctggaa 6780
actactaatc aggcaattga ggcaatcaga caagcagggc aggagatgat attggctgtt 6840
cagggtgtcc aagactacat caataatgag ctgataccgt ctatgaacca actatcttgt 6900
gatttaatcg gccagaagct agggctcaaa ttgctcagat actatacaga aatcctgtca 6960
ttatttggcc ccagcttacg ggaccccata tctgcggaga tatccatcca ggctttgagc 7020
tatgcgcttg gaggagatat caataaggta ttagaaaagc tcggatacag tggaggtgat 7080
ttactgggca tcttagagag cagaggaata aaggcccgga taactcacgt cgacacagag 7140
tcctacttca ttgtactcag tatagcctat ccgacgctgt ccgagattaa gggggtgatt 7200
gtccaccggc tagagggggt ctcgtacaat ataggctctc aagagtggta taccactgtg 7260
cccaagtatg ttgcaaccca agggtacctt atctcgaatt ttgatgagtc atcgtgtact 7320
ttcatgccag aggggactgt gtgcagccaa aatgccttgt acccgatgag tcctctgctc 7380
caagaatgcc tccgggggtc caccaagtcc tgtgctcgta cactcgtatc cgggtctttt 7440
gggaaccggt tcattttatc acaagggaac ctaatagcca attgtgcatc aatcctctgc 7500
aagtgttaca caacaggaac gatcattaat caagaccctg acaagatcct aacatacatt 7560
gctgccgatc actgcccggt ggtcgaggtg aacggtgtga ccatccaagt cgggagcagg 7620
aggtatccgg acgcggtgta cctgcacaga attgacctcg gtcctcccat atcattggag 7680
aggttggacg tagggacaaa tctggggaat gcaattgcta agttggagga tgccaaggaa 7740
ttgttggagt catcggacca gatattgagg agtatgaaag gtttatcgag cactagcata 7800
gtttacatcc tgattgcagt gtgtcttgga gggttgatag ggatccccgc tttaatatgt 7860
tgctgcaggg ggcgctgtaa caaaaaggga gaacaagttg gtatgtcaag accaggccta 7920
aagcctgatc ttacagggac atcaaaatcc tatgtaaggt cgctctgatc ctctacaact 7980
cttgaaacac agatttccca caagtctcct cttcgtcatc aagcaaccac cgcatccagc 8040
atcaagccca cctgaaattg tctccggctt ccctctggcc gaacgatatc ggtagttaat 8100
taaaacttag ggtgcaagat catccacaat gtcaccacaa cgagaccgaa taaatgcctt 8160
ctacaaagac aacccacatc ctaagggaag taggatagtt attaacagag aacatcttat 8220
gattgataga ccttatgttt tgctggctgt tctattcgtc atgtttctga gcttgatcgg 8280
gttgctagcc attgcaggca ttagactcca tcgtgcagcc atctacaccg cagagatcca 8340
taagagcctc agcaccaatc tagatgtaac taactcgatc gagcatcagg tcaaggacgt 8400
gctgacacca ctcttcaaga tcattggtga tgaagtgggc ctgaggacac ctcagagatt 8460
cactgaccta gtgaaattca tctctgacaa aattaaattc cttaatccgg atagggagta 8520
cgacttcaga gatctcactt ggtgtatcaa cccgccagag agaatcaaat tggattatga 8580
tcaatactgt gcagatgtgg ctgctgaaga actcatgaat gcattggtga actcaactct 8640
actggaggcc agggcaacca atcagttcct agctgtctca aagggaaact gctcagggcc 8700
cactacaatc agaggtcaat tctcaaacat gtcgctgtcc ctgttggact tgtatttaag 8760
tcgaggttac aatgtgtcat ctatagtcac tatgacatcc cagggaatgt acgggggaac 8820
ttacctagtg ggaaagccta atctgagcag taaagggtca gagttgtcac aactgagcat 8880
gcaccgagtg tttgaagtag gggttatcag aaatccgggt ttgggggctc cggtgttcca 8940
tatgacaaac tattttgagc aaccagtcag taatgatttc agcaactgca tggtggcttt 9000
gggggagctt aaattcgcag ccctctgtca cagggaagat tctatcacaa ttccctatca 9060
ggggtcaggg aaaggtgtca gcttccagct cgtcaagcta ggtgtctgga aatccccaac 9120
cgacatgcga tcctgggtcc ccctatcaac ggatgatcca gtgatagata ggctttatct 9180
ctcatctcac agaggtgtta tcgctgacaa tcaagcaaaa tgggctgtcc cgacaacacg 9240
gacagatgac aagttgcgaa tggagacatg cttccagcag gcgtgtaagg gtaaaaacca 9300
agcactctgc gagaatcccg agtgggcacc attgaaggat aacaggattc cttcatacgg 9360
ggtcttgtct gttaatctga gtctgacagt tgagcttaaa atcaaaattg cttcaggatt 9420
cgggccattg atcacacacg gttcagggat ggacctatac aaaaccaacc acaacaatgt 9480
gtattggctg actatcccgc caatgaagaa cctagcctta ggtgtaatca acacattgga 9540
gtggataccg agattcaagg ttagtcccaa cctcttcact gttccaatca aggaagcagg 9600
cgaggactgc catgccccaa catacctacc tgcggaggtg gatggtgatg tcaaactcag 9660
ttccaatctg gtaattctac ctggtcagga tctccaatat gttttggcaa cctacgatac 9720
ttccagggtt gaacatgctg tggtttatta tgtttacagc ccaagccgct cattttctta 9780
cttttatcct tttaggttgc ctataaaggg ggtcccaatc gaattacaag tggaatgctt 9840
cacatgggac aaaaaactct ggtgccgtca cttctgtgtg cttgcggact cagaatctgg 9900
tggacatatc actcactctg ggatggtggg catgggagtc agctgcacag tcactcggga 9960
agatggaacc aatcgcagat agggctgcca gtgaaccgat cacatgatgt cacccagaca 10020
tcaggcatac ccactagtgt gaaatagaca tcagaattaa gaaaaacgta gggtccaagt 10080
ggtttcccgt tatggactcg ctatctgtca accagatctt ataccctgaa gttcacctag 10140
atagcccgat agttaccaat aagatagtag ctatcctgga gtatgctcga gtccctcacg 10200
cttacagcct ggaggaccct acactgtgtc agaacatcaa gcaccgccta aaaaacggat 10260
tctccaacca aatgattata aacaatgtgg aagttgggaa tgtcatcaag tccaagctta 10320
ggagttatcc ggcccactct catattccat atccaaattg taatcaggat ttatttaaca 10380
tagaagacaa agagtcaaca aggaagatcc gtgagctcct aaaaaaggga aattcgctgt 10440
actccaaagt cagtgataag gttttccaat gcctgaggga cactaactca cggcttggcc 10500
taggctccga attgagggag gacatcaagg agaaaattat taacttggga gtttacatgc 10560
acagctccca atggtttgag ccctttctgt tttggtttac agtcaagact gagatgaggt 10620
cagtgattaa atcacaaacc catacttgcc ataggaggag acacacacct gtattcttca 10680
ctggtagttc agttgagctg ttaatctctc gtgaccttgt tgctataatc agtaaggaat 10740
ctcaacatgt atattacctg acgtttgaac tggttttgat gtattgtgat gtcatagagg 10800
ggaggttaat gacagagacc gctatgacca ttgatgctag gtatgcagaa cttctaggaa 10860
gagtcagata catgtggaaa ctgatagatg gtttcttccc tgcactcggg aatccaactt 10920
atcaaattgt agccatgctg gagccacttt cacttgctta cctgcaactg agggatataa 10980
cagtagaact cagaggtgct ttccttaacc actgctttac tgaaatacat gatgttcttg 11040
accaaaacgg gttttctgat gaaggtactt atcatgagtt aattgaagcc ctagattaca 11100
ttttcataac tgatgacata catctgacag gggagatttt ctcatttttc agaagtttcg 11160
gccaccccag acttgaagca gtaacggctg ctgaaaatgt caggaaatac atgaatcagc 11220
ctaaagtcat tgtgtatgag actctgatga aaggtcatgc catattttgt ggaatcataa 11280
tcaacggcta tcgtgacagg cacggaggca gttggccacc cctgaccctc cccctgcatg 11340
ctgcagacac aatccggaat gctcaagctt caggtgaagg gttaacacat gagcagtgcg 11400
ttgataactg gaaatcattt gctggagtga gatttggctg ttttatgcct cttagcctgg 11460
acagtgatct gacaatgtac ctaaaggaca aggcacttgc tgctctccaa agggaatggg 11520
attcagttta cccgaaagag ttcctgcgtt acgatcctcc caagggaacc gggtcacgga 11580
ggcttgtaga tgttttcctt aatgattcga gctttgaccc atatgatatg ataatgtatg 11640
tcgtaagtgg agcctacctc catgaccctg agttcaacct gtcttacagc ctgaaagaaa 11700
aggagatcaa ggaaacaggt agacttttcg ctaaaatgac ttacaaaatg agggcatgcc 11760
aagtgatcgc tgaaaatcta atctcaaacg ggattggcaa gtattttaag gacaatggga 11820
tggccaagga tgagcacgat ttgactaagg cactccacac tctggctgtc tcaggagtcc 11880
ccaaagatct caaagaaagt cacagggggg ggccagtctt aaaaacctac tcccgaagcc 11940
cagtccacac aagtaccagg aacgttaaag cagaaaaagg gtttgtagga ttccctcatg 12000
taattcggca gaatcaagac actgatcatc cggagaatat agaaacctac gagacagtca 12060
gcgcatttat cacgactgat ctcaagaagt actgccttaa ttggagatat gagaccatca 12120
gcttatttgc acagaggcta aatgagattt acggattacc ctcatttttt cagtggctgc 12180
ataagaggct tgaaacctct gtcctctatg taagtgaccc tcattgcccc cccgaccttg 12240
acgcccatgt cccgttatgc aaagtcccca atgaccaaat cttcatcaag taccctatgg 12300
gaggtataga agggtattgt cagaagctgt ggaccatcag caccattccc tacttatacc 12360
tggctgctta tgagagcggg gtaaggattg cttcgttagt gcaaggggac aatcagacca 12420
tagccgtaac aaaaagggta cccagcacat ggccttacaa ccttaagaaa cgggaagctg 12480
ctagagtaac tagagattac tttgtaattc ttaggcaaag gctacatgac attggccatc 12540
acctcaaggc aaatgagaca attgtttcat cacatttttt tgtctattca aaaggaatat 12600
attatgatgg gctacttgtg tcccaatcac tcaagagcat cgcaagatgt gtattctggt 12660
cagagactat agttgatgaa acaagggcgg catgcagtaa tattgctaca acaatggcca 12720
aaagcattga gagaggttat gaccgttatc ttgcatattc cctgaacgtc ctaaaagtga 12780
tacagcaaat tttgatctcc cttggcttca caatcaattc aaccatgacc cgggatgtag 12840
tcatacccct cctcacaaac aacgatctct taataaggat ggcactgttg cccgctccta 12900
ttgggggtat gaattatctg aatatgagca ggctgtttgt cagaaacatc ggtgatccag 12960
taacatcatc aattgctgat ctcaagagaa tgattctcgc atcactaatg cctgaagaga 13020
ccctccatca agtaatgaca caacaaccgg gggactcttc attcctagac tgggctagcg 13080
acccttactc agcaaatctt gtatgcgtcc agagcatcac tagactcctc aagaacataa 13140
ctgcaaggtt tgtcctgatc cacagtccaa acccaatgtt aaaagggtta ttccatgatg 13200
acagcaaaga agaggacgag agactggcgg cattcctcat ggacaggcat attatagtac 13260
ctagggcagc tcatgaaatc ctggatcata gtgtcacagg ggcaagagag tctattgcag 13320
gcatgctaga taccacaaaa ggcctgattc gagccagcat gaggaagggg gggttaacct 13380
ctcgagtgat aaccagattg tccaattatg actatgaaca atttagagca gggatggtgc 13440
tattgacagg aagaaagaga aatgtcctca ttgacaaaga gtcatgttca gtgcagctgg 13500
ctagagccct aagaagccat atgtgggcaa gactagctcg aggacggcct atttacggcc 13560
ttgaggtccc tgatgtacta gaatctatgc gaggccacct tattcggcgt catgagacat 13620
gtgtcatctg cgagtgtgga tcagtcaact acggatggtt ttttgtcccc tcgggttgcc 13680
aactggatga tattgacaag gaaacatcat ccttgagagt cccatatatt ggttctacca 13740
ctgatgagag aacagacatg aagctcgcct tcgtaagagc cccaagtaga tccttgcgat 13800
ctgccgttag aatagcaaca gtgtactcat gggcttacgg tgatgatgat agctcttgga 13860
acgaagcctg gttgttggca aggcaaaggg ccaatgtgag cctggaggag ctaagggtga 13920
tcactcccat ctcgacttcg actaatttag cgcataggtt gagggatcgt agcactcaag 13980
tgaaatactc aggtacatcc cttgtccgag tggcaaggta taccacaatc tccaacgaca 14040
atctctcatt tgtcatatca gataagaagg ttgatactaa ctttatatac caacaaggaa 14100
tgcttctagg gttgggtgtt ttagaaacat tgtttcgact cgagaaagat actggatcat 14160
ctaacacggt attacatctt cacgtcgaaa cagattgttg cgtgatcccg atgatagatc 14220
atcccaggat acccagctcc cgcaagctag agctgagggc agagctatgt accaacccat 14280
tgatatatga taatgcacct ttaattgaca gagatgcaac aaggctatac acccagagcc 14340
ataggaggca ccttgtggaa tttgttacat ggtccacacc ccaactatat cacattctag 14400
ctaagtccac agcactatct atgattgacc tggtaacaaa atttgagaag gaccatatga 14460
atgaaatttc agctctcata ggggatgacg atatcaatag tttcataact gagtttctgc 14520
ttatagagcc aagattattc accatctact tgggccagtg tgcagccatc aattgggcat 14580
ttgatgtaca ttatcataga ccatcaggga aatatcagat gggtgagctg ttgtcttcgt 14640
tcctttctag aatgagcaaa ggagtgttta aggtgcttgt caatgctcta agccacccaa 14700
agatctacaa gaaattctgg cattgtggta ttatagagcc tatccatggt ccttcacttg 14760
atgctcaaaa cttgcacaca actgtgtgca acatggttta cacatgctat atgacctacc 14820
tcgacctgtt gttgaatgaa gagttagaag agttcacatt tcttttgtgt gaaagcgatg 14880
aggatgtagt accggacaga ttcgacaaca tccaggcaaa acacttgtgt gttctggcag 14940
atttgtactg tcaaccaggg acctgcccac cgattcgagg tctaaggccg gtagagaaat 15000
gtgcagttct aaccgatcat atcaaggcag aggctaggtt atctccagca ggatcttcgt 15060
ggaacataaa tccaattatt gtagaccatt actcatgctc tctgacttat ctccgtcgag 15120
gatctatcaa acagataaga ttgagagttg atccaggatt catttttgac gccctcgctg 15180
aggtaaatgt cagtcagcca aaggtcggca gcgacgtcaa gatggacaaa aagactatag 15240
tttggtttag aagagaccta aggattgagg ataatcctgc attagcagca gctgctcacg 15300
aaggatctgt ttttcctgtc ttcatttggt gtcctgaaga agaaggacag ttttatcctg 15360
gaagagcttc aagatggtgg atgaaacaat cacttgctca cttatctcaa tccttgaagg 15420
ctcttggatc tgacctcact ttaatcaaaa cccacaacac gatttcagcg atcttggatt 15480
gtatccgcgt taccggtgct acaaaagtcg tctttaacca cctctatgat cctgtttcgt 15540
tagttcggga ccataccgta aaggagaagc tggtggaacg tgggatctct gtgcaaagct 15600
acaatggaga tctattgtat gaaccgtggg agatatactg cgaaaagggc aaacctttta 15660
cgagtttcaa ttcttactgg aagaaatgct tagatatgtc gattgaatcc gttatgcttc 15720
ctcctccttg gcggttgatg ccaataactg cagcggctga agcgatttgg gcgtgttcga 15780
ttgaagaact agggctggag aatgaggccg agaaaccgag caatgcgttg ttaactagag 15840
cttggtctcc aggatggagc aatgctgata agttactaaa tgagttcatc gagaagcagt 15900
tgatagatta tgcaaagaac agcaagaaag ttgttgggaa ttctacttca ctactttctc 15960
cgtatctcca tttcggggaa ataagcgtca gacacgtttt ccagtgtgcc cggatgaaac 16020
aaattatatg ggcaagagat aagaacagtg aaggagaaga aagtgcagat ctttttctta 16080
ggggaatcgg tttaagagag tattctcggt atatatgttt caacttcccg tttactcacg 16140
agcaatcgtt gttgagtcat cttcggtttt tcccttggga tgctgatgtt gataagttca 16200
aggcctggag acaaggcagg accggttatc cgttggtgga tgccggaatg agagagcttt 16260
gggctaccgg atggatgcat aacagaataa gagtgattgt ttcaagcttt gctgtgaagt 16320
ttcttctcct tccatggaaa tggggaatga agtatttctg ggatacactt ttggatgctg 16380
atttggaatg tgacatcctt ggctggcagt atatctctgg gagtatcccc gatggccacg 16440
agcttgatcg cttggacaat cccgcgttac aaggcgccaa atatgaccca gaaggtgagt 16500
acataaggca atggcttccc gagcttgcga gattgccaac tgaatggatc catcatccat 16560
gggacgctcc tttaaccgta ctcaaagctt ctggtgtgga actcggaaca aactatgcga 16620
aacccattgt agacatcgac acagctcgtg agctactagc taaagctatt tcaagaaccc 16680
gtgaagcaca gatcatgatc ggagcagcaa ccggtaattc cgctgacggc ggcggaggat 16740
cgggtggtag tggtggttca ggaggaggat cgacccaagg aggatccatg aatggagcta 16800
taggaggtga ccttttgctc aattttcctg acatgtcggt cctagagcgc caaagggctc 16860
acctcaagta cctcaatccc acctttgatt ctcctctcgc cggcttcttt gccgattctt 16920
caatgattac cggcggcgag atggacagct atctttcgac tgccggtttg aatcttccga 16980
tgatgtacgg tgagacgacg gtggaaggtg attcaagact ctcaatttcg ccggaaacga 17040
cgcttgggac tggaaatttc aaggcagcga agtttgatac agagactaag gattgtaatg 17100
aggcggcgaa gaagatgacg atgaacagag atgacctagt agaagaagga gaagaagaga 17160
agtcgaaaat aacagagcaa aacaatggga gcacaaaaag catcaagaag atgaaacaca 17220
aagccaagaa agaagagaac aatttctcta atgattcatc taaagtgacg aaggaattgg 17280
agaaaacgga ttatattggc tatccgtacg acgtcccaga ttacgcgggc atctcaaata 17340
tgagcatcaa ggatttcaga cctccacacg atgatgttgc aaaattgctc aaagatatca 17400
acacaagcaa gcacaatctt cccatttcag ggggtagtct cgccaattat gaaatccatg 17460
ctttccgcag aatcgggtta aactcatctg cttgctacaa agctgttgag atatcaacat 17520
taattaggag atgccttgag ccaggggaag acggcttgtt cttgggtgag gggtcgggtt 17580
ctatgttgat cacttataag gagatactaa aactaaacaa gtgcttctat aatagtgggg 17640
tttccgccaa ttctagatct ggtcaaaggg aattagcacc ctatccctcc gaagttggcc 17700
ttgtcgaaca cagaatggga gtaggtaata ttgtcaaggt gctctttaac gggaggcccg 17760
aagtcacgtg ggtaggcagt atagattgct tcaatttcat agtcagtaat atccctacct 17820
ctagtgtggg gtttatccat tcagatatag agaccttacc taacaaagat actatagaga 17880
agctagagga attggcagcc atcttatcga tggctctact ccttggcaaa ataggatcaa 17940
tactggtgat taagcttatg cctttcagcg gggattttgt tcagggattt ataagctatg 18000
tagggtctca ttatagagaa gtgaaccttg tctaccctag gtacagcaac ttcatatcta 18060
ctgaatctta tttagttatg acagatctca aagctaaccg gctaatgaat cctgaaaaga 18120
tcaagcagca gataattgaa tcatctgtac ggacttcacc tggacttata ggtcacatcc 18180
tatccattaa gcaactaagc tgcatacaag caattgtggg aggcgcggtt agtagaggtg 18240
atatcaaccc tattctgaaa aaacttacac ctatagagca ggtgctgatc agttgcgggt 18300
tggcaattaa cggacctaaa ctgtgcaaag aattaatcca ccatgatgtt gcctcagggc 18360
aagatggatt gcttaactct atactcatcc tctacaggga gttggcaaga ttcaaagaca 18420
accaaagaag tcaacaaggg atgttccacg cttaccccgt attggtaagt agtaggcaac 18480
gagaacttgt atctaggatc actcgcaaat tttgggggca tattcttctt tactccggga 18540
acagaaagtt gataaatcgg tttatccaga atctcaagtc cggttatcta gtactagact 18600
tacaccagaa tatcttcgtt aagaatctat ccaagtcaga gaaacagatt attatgacgg 18660
ggggtttaaa acgtgagtgg gtttttaagg taacagtcaa ggagaccaaa gaatggtaca 18720
agttagtcgg atacagcgct ctgattaagg attaattggt tgaactccgg aaccctaatc 18780
ctgccctagg tagttaggca ttatttgcaa tatattaaag aaaactttga aaatacgaag 18840
tttctattcc cagctttgtc tggtggccgg catggtccca gcctcctcgc tggcgccggc 18900
tgggcaacat tccgagggga ccgtcccctc ggtaatggcg aatgggacgc ggccgccgcg 18960
ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 19020
tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 19080
tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 19140
attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 19200
agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 19260
tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 19320
ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 19380
ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 19440
aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 19500
ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 19560
attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 19620
ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 19680
cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 19740
cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 19800
aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 19860
ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 19920
gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 19980
cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 20040
acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 20100
gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 20160
aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 20220
ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 20280
ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 20340
gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 20400
aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 20460
aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 20520
aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 20580
gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 20640
gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 20700
tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 20760
gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 20820
caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 20880
actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 20940
acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 21000
cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 21060
gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 21120
cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 21180
gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 21240
tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 21300
tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 21360
gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 21420
aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 21480
agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 21540
cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 21600
gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 21660
atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 21720
agctatgacc atgattacgc caagcgcgcc agtgaattgt aatacgactc actata 21776
<210> 17
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> EGFP 正向引物
<400> 17
ggcgcgccat ggtgagcaag ggcgaggag 29
<210> 18
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> EGFP 反向引物
<400> 18
gacgtcttac ttgtacagct cgtccatgcc 30
<210> 19
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> DP 正向1引物
<400> 19
ggtcggcagc gacgtcatgg tgagcgtgat caagc 35
<210> 20
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> DP 正向2引物
<400> 20
atggtgagcg tgatcaagcc cg 22
<210> 21
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> DP 反向1引物
<400> 21
gatcacgctc accatggatc ctccttgggt cgatcctcct 40
<210> 22
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> DP 反向2引物
<400> 22
ctcatatttg agatgacgtc cttggcctgg cggggca 37
<210> 23
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> pMagnMaghigh 正向1引物
<400> 23
caaaggtcgg cagcgacgtc agccatactc tttatgcccc cggt 44
<210> 24
<211> 61
<212> DNA
<213> Artificial Sequence
<220>
<223> pMagnMaghigh 正向2引物
<400> 24
gggtggtagt ggtggttcag gaggaggatc gacccaagga ggatccatgc atactcttta 60
t 61
<210> 25
<211> 61
<212> DNA
<213> Artificial Sequence
<220>
<223> pMagnMaghigh 反向2引物
<400> 25
ccaccactac cacccgatcc tccgccgccg tcagcggaat taccggtttc tgtttcgcac 60
t 61
<210> 26
<211> 49
<212> DNA
<213> Artificial Sequence
<220>
<223> pMagnMaghigh 反向2引物
<400> 26
cccgcgtaat ctgggacgtc gtacggatat tctgtttcgc actggaatc 49
<210> 27
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> 反向3引物
<400> 27
ctcatatttg agatgcccgc gtaatctggg a 31
<210> 28
<211> 48
<212> DNA
<213> Artificial Sequence
<220>
<223> CRY2-CIB1N 正向1引物
<400> 28
caaaggtcgg cagcgacgtc aagatggaca aaaagactat agtttggt 48
<210> 29
<211> 61
<212> DNA
<213> Artificial Sequence
<220>
<223> CRY2-CIB1N 正向2引物
<400> 29
gggtggtagt ggtggttcag gaggaggatc gacccaagga ggatccatga atggagctat 60
a 61
<210> 30
<211> 64
<212> DNA
<213> Artificial Sequence
<220>
<223> CRY2-CIB1N 反向1引物
<400> 30
ccaccactac cacccgatcc tccgccgccg tcagcggaat taccggtttt gcaaccattt 60
tttc 64
<210> 31
<211> 54
<212> DNA
<213> Artificial Sequence
<220>
<223> CRY2-CIB1N 反向2引物
<400> 31
cccgcgtaat ctgggacgtc gtacggatag ccaatataat ccgttttctc caat 54
<210> 32
<211> 62
<212> DNA
<213> Artificial Sequence
<220>
<223> CRYPHR-CIB1N 反向1引物
<400> 32
ccaccactac cacccgatcc tccgccgccg tcagcggaat taccggttgc tgctccgatc 60
at 62
<210> 33
<211> 5313
<212> DNA
<213> Artificial Sequence
<220>
<223> pCITE-IC-N
<400> 33
gggcgaatta attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc 60
ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag 120
gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac 180
aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc 240
tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc 300
acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca 360
aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt 420
gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg 480
ggacgtggtt ttcctttgaa aaacacgatg ataataccat ggccacactt ttgaggagct 540
tagcattgtt caaaagaaac aaggacaaac cacccattac atcaggatcc ggtggagcca 600
tcagaggaat caaacacatt attatagtac caattcctgg agattcctca attaccactc 660
gatccagact actggaccgg ttggtcaggt taattggaaa cccggatgtg agcgggccca 720
aactaacagg ggcactaata ggtatattat ccttatttgt ggagtctcca ggtcaattga 780
ttcagaggat caccgatgac cctgacgtta gcatcaggct gttagaggtt gttcagagtg 840
accagtcaca atctggcctt accttcgcat caagaggtac caacatggag gatgaggcgg 900
accaatactt ttcacatgat gatccaagca gtagtgatca atccaggtcc ggatggttcg 960
agaacaagga aatctcagat attgaagtgc aagaccctga gggattcaac atgattctgg 1020
gtaccattct agcccagatc tgggtcttgc tcgcaaaggc ggttacggcc ccagacacgg 1080
cagctgattc ggagctaaga aggtggataa agtacaccca acaaagaagg gtagttggtg 1140
aatttagatt ggagagaaaa tggttggatg tggtgaggaa caggattgcc gaggacctct 1200
ctttacgccg attcatggtg gctctaatcc tggatatcaa gaggacaccc gggaacaaac 1260
ctaggattgc tgaaatgata tgtgacattg atacatatat cgtagaggca ggattagcca 1320
gttttatcct gactattaag tttgggatag aaactatgta tcctgctctt ggactgcatg 1380
aatttgctgg tgagttatcc acacttgagt ccttgatgaa tctttaccag caaatgggag 1440
aaactgcacc ctacatggta atcctagaga actcaattca gaacaagttc agtgcaggat 1500
cataccctct gctctggagc tatgccatgg gagtaggagt ggaacttgaa aactccatgg 1560
gaggtttgaa ctttggtcga tcttactttg atccagcata ttttagatta gggcaagaga 1620
tggtgaggag gtcagctgga aaggtcagtt ccacattggc atccgaactc ggtatcactg 1680
ccgaggatgc aaggcttgtt tcagagattg caatgcatac tactgaggac aggatcagta 1740
gagcggtcgg acccagacaa gcccaagtgt catttctaca cggtgatcaa agtgagaatg 1800
agctaccagg attggggggc aaggaagata ggagggtcaa acagggtcgg ggagaagcca 1860
gggagagcta cagagaaacc gggtccagca gagcaagtga tgcgagagct gcccatcctc 1920
caaccagcat gcccctagac attgacactg catcggagtc aggccaagat ccgcaggaca 1980
gtcgaaggtc agctgacgcc ctgctcaggc tgcaagccat ggcaggaatc ttggaagaac 2040
aaggctcaga cacggacacc cctagggtat acaatgacag agatcttcta gactagctcg 2100
agcaccacca ccaccccact gagatctccg atcctctaga gtcgatcgac ctgcaggcat 2160
gcaagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc atggtcatag 2220
ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 2280
ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 2340
tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 2400
cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 2460
ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 2520
ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 2580
gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 2640
gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 2700
taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 2760
accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 2820
tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 2880
cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 2940
agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 3000
gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 3060
gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 3120
tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 3180
acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 3240
cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 3300
acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 3360
acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 3420
tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 3480
ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 3540
ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 3600
tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 3660
aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 3720
ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 3780
ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 3840
gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 3900
gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 3960
cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 4020
actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 4080
ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 4140
tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 4200
ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 4260
agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 4320
aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 4380
attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 4440
cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 4500
tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 4560
gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 4620
atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt 4680
aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 4740
ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 4800
gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 4860
agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 4920
ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 4980
tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 5040
agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 5100
cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg ccattcaggc tgcgcaactg 5160
ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 5220
tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 5280
gacggccagt gaattgtaat acgactcact ata 5313
<210> 34
<211> 5259
<212> DNA
<213> Artificial Sequence
<220>
<223> pCITE-IC-PdeltaC
<400> 34
gggcgaatta attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc 60
ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag 120
gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac 180
aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc 240
tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc 300
acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca 360
aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt 420
gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg 480
ggacgtggtt ttcctttgaa aaacacgatg ataataccat ggcagaagag caggcacgcc 540
atgtgaaaaa cggactagaa tgcatccggg ctctcaaggc cgagcccatc ggctcactgg 600
ccgtcgagga agccatggca gcatggtcag aaatatcaga caacccagga caggaccgag 660
ccacctgcaa ggaagaggag gcaggcagtt cgggtctcag caaaccatgc ctctcagcaa 720
ttggatcaac tgaaggcggt gcacctcgca tccgcggtca gggatctgga gaaagcgatg 780
acgacgctga aactttggga atcccctcaa gaaatctcca ggcatcaagc actgggttac 840
agtgttatca tgtttatgat cacagcggtg aagcggttaa gggaatccaa gatgctgact 900
ctatcatggt tcaatcaggc cttgatggtg atagcaccct ctcaggagga gacgatgaat 960
ctgaaaacag cgatgtggat attggcgaac ctgataccga gggatatgct atcactgacc 1020
ggggatctgc tcccatctct atggggttca gggcttctga tgttgaaact gcagaaggag 1080
gggagatcca cgagctcctg aaactccaat ccagaggcaa caactttccg aagcttggga 1140
aaactctcaa tgttcctccg cccccgaacc ccagtagggc cagcacttcc gagacaccca 1200
ttaaaaaggg cacagacgcg agattggcct catttggaac ggagatcgcg tctttattga 1260
caggtggtgc aacccaatgt gctcgaaagt caccctcgga accatcaggg ccaggtgcac 1320
ctgcggggaa tgtccccgag tgtgtgagca atgccgcact gatacaggag tggacacccg 1380
aatctggtac cacaatctcc ccgagatccc agaataatga agaaggggga gactattatg 1440
atgatgagct gttctccgat gtccaagaca tcaaaacagc cttggccaaa atacacgagg 1500
ataatcagaa gataatctcc aagctagaat cattgctgtt attgaaggga gaagttgagt 1560
caattaagaa gcagatcaac aggcaaaata tcagcatatc caccctggaa ggacacctct 1620
caagcatcat gattgccatt cctggacttg ggaaggatcc caacgacccc actgcagatg 1680
tcgaactcaa tcccgacctg aaacccatca taggcagaga ttcaggccga gcactggccg 1740
aagttctcaa gaagcccgtt gccagccgac aactccaggg aatgactaat ggacggacca 1800
gttccagagg acagctgctg aaggaatttc aactaaagcc gatcgggaaa aaggtgagct 1860
cagccgtcgg gtttgttcct gacaccggcc ctgcatcacg cagtgtaatc cgctccatta 1920
taaaatccag ccggctagag gaggatcgga agcgttacct gatgactctc cttgatgata 1980
tcaaaggagc caacgatctt gccaagttcc accagatgct gatgaagata ataatgaagt 2040
agctcgagca ccaccaccac cccactgaga tctccgatcc tctagagtcg atcgacctgc 2100
aggcatgcaa gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg 2160
tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 2220
ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 2280
ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 2340
ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 2400
gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 2460
atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 2520
caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 2580
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 2640
taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 2700
ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 2760
tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 2820
gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 2880
ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 2940
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 3000
aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 3060
agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 3120
cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 3180
gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 3240
atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 3300
gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 3360
tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 3420
gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 3480
ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 3540
actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 3600
ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 3660
tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 3720
cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 3780
ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 3840
ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 3900
tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 3960
agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 4020
atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 4080
gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 4140
aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 4200
tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 4260
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 4320
gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 4380
ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 4440
acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 4500
gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg 4560
caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga 4620
aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 4680
ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 4740
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 4800
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 4860
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 4920
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 4980
gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 5040
acccgccgcg cttaatgcgc cgctacaggg cgcgtcgcgc cattcgccat tcaggctgcg 5100
caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 5160
gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 5220
taaaacgacg gccagtgaat tgtaatacga ctcactata 5259
<210> 35
<211> 10330
<212> DNA
<213> Artificial Sequence
<220>
<223> pCITE-9301B-wtL
<400> 35
gggcgaatta attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc 60
ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag 120
gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac 180
aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc 240
tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc 300
acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca 360
aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt 420
gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg 480
ggacgtggtt ttcctttgaa aaacacgatg ataataccat ggaattcatg gactcgctat 540
ctgtcaacca gatcctatac cctgaagttc acctagatag cccgatagtt accaataaga 600
tagtagctat cctggagtat gctcgagtcc ctcacgctta cagcctggag gaccctacac 660
tgtgtcagaa catcaagcac cgcctaaaaa acggattctc caaccaaatg attataaaca 720
atgtggaagt tgggaatgtc atcaagtcca agcttaggag ttatccggcc cactctcata 780
ttccatatcc aaattgtaat caggatttat ttaacataga agacaaagag tcaacaagga 840
agatccgtga gctcctaaaa aagggaaatt cgctgtactc caaggtcagt gataaggttt 900
tccaatgcct gagggacact aattcacggc ttggcttagg ctctgaattg agagaggaca 960
tcaaggagaa aattattaac ttgggagttt acatgcacag ctcccaatgg tttgagccct 1020
ttctgttttg gtttacagtc aagactgaga tgaggtcagt gatcaaatca caaacccata 1080
cttgccatag gaggagacac acacctgtat tcttcactgg tagttcagtt gagctgttaa 1140
tctctcgtga ccttgttgct ataattagta aagagtctca acatgtatat tacctgacgt 1200
ttgaactggt tttgatgtat tgtgatgtca tagaggggag gttaatgaca gagaccgcta 1260
tgaccattga tgctaggtat gcagaacttc taggaagagt cagatacatg tggaaactga 1320
tagatggttt cttccctgca ctcgggaacc caacttatca aattgtagcc atgctggagc 1380
cactttcact tgcttacctg caactgaggg atataacagt agaactcaga ggtgctttcc 1440
ttaaccactg ctttactgaa atacatgatg ttcttgacca aaacgggttt tctgatgaag 1500
gtacttatca tgagttaatt gaagccctag attacatttt cataactgat gacatacatt 1560
tgacagggga gattttctca tttttcagaa gtttcggcca ccccagactt gaagcagtaa 1620
cggctgctga aaatgttagg aaatacatga atcagcctaa agtcattgtg tatgagactc 1680
tgatgaaagg tcatgccata ttttgtggaa tcataatcaa cggctatcgt gacaggcacg 1740
gaggcagttg gccacccctg accctccccc tgcatgctgc agacacaatc cggaatgccc 1800
aagcctcagg cgaagggtta acacatgagc agtgcgttga taactggaaa tcatttgctg 1860
gagtgagatt tggctgtttt atgcctctta gcctggacag tgatctgaca atgtacctaa 1920
aggacaaggc acttgctgct ctccaaaggg aatgggattc agtttacccg aaagagtttc 1980
tgcgttacga tcctcccaag ggaaccgggt cacggaggct tgtagatgtt ttccttaatg 2040
attcgagctt tgacccatat gatatgataa tgtatgtcgt aagtggagct tacctccatg 2100
actctgagtt caacctgtct tacagcctga aagaaaagga gatcaaggaa acaggtagac 2160
tttttgctaa aatgacttac aaaatgaggg catgccaagt gattgctgaa aatctaatct 2220
caaacgggat tggcaaatat tttaaggaca atgggatggc caaggatgag cacgatttga 2280
ccaaggcact ccacactcta gctgtctcag gagtccccaa agatctcaaa gaaagtcaca 2340
ggggggggcc agtcttaaaa acctactccc gaagcccagt ccacacaagt accaggaacg 2400
ttaaagcagc aaaagggttt gtaggattcc ctcatgtaat tcgggagaat caagacactg 2460
atcatccgga gaatatagaa acttacgaga cagtcagcgc atttatcacg actgatctca 2520
agaagtactg ccttaattgg agatatgaga ccatcagctt atttgcacag aggctaaatg 2580
agatttacgg attaccctca ttttttcagt ggctgcataa gaggcttgaa acctctgtcc 2640
tctatgtaag tgaccctcat tgcccccccg accttgacgc ccatgtcccg ttatgcaaag 2700
tccctaatga ccaaatcttc atcaagtacc ctatgggagg catagaaggg tattgtcaga 2760
agttgtggac catcagcacc attccctact tatacctggc tgcttatgag agcggagtaa 2820
ggattgcttc gttagtgcaa ggggacaatc agaccatagc cgtaacaaaa agggtaccca 2880
gcacatggcc ttacaacctt aagaaacggg aagctgctag agtaactaga gattactttg 2940
taattcttag gcaaaggcta catgacattg gccatcacct caaggcaaat gagacaattg 3000
tttcatcaca tttttttgtc tattcaaaag gaatatatta tgatgggcta cttgtgtccc 3060
aatcactcaa gagcatcgca agatgtgtat tctggtcaga gactatagtt gatgaaacaa 3120
gggcggcatg cagtaatatt gctacaacaa tggccaaaag cattgagaga ggttatgacc 3180
gttatcttgc atattccctg aacgtcctaa aagtgataca gcaaattttg atctcccttg 3240
gcttcacaat caattcaacc atgacccggg atgtagtcat acccctcctc acaaacaacg 3300
atctcttaat aaggatggca ctgttgcccg ctcctattgg gggtatgaat tatctgaata 3360
tgagcaggct gtttgtcaga aacatcggtg atccagtaac atcatcaatt gctgatctca 3420
agagaatgat tctcgcatca ctaatgcctg aagagaccct ccatcaagta atgacacaac 3480
aaccggggga ctcttcattc ctagactggg ctagcgaccc ttactcagca aatcttgtat 3540
gcgtccagag catcactaga ctcctcaaga acataactgc aaggtttgtc ctgatccaca 3600
gtccaaaccc aatgttaaaa gggttattcc atgatgacag caaagaagag gacgagagac 3660
tggcggcatt cctcatggac aggcatatta tagtacctag ggcagctcat gaaatcctgg 3720
atcatagtgt cacaggggca agagagtcta ttgcaggcat gctagatacc acaaaaggcc 3780
tgattcgagc cagcatgagg aaaggggggt taacctctcg agtgataacc agattgtcca 3840
attatgacta tgaacaattt agagcaggga tggtgctatt gacaggaaga aagagaaatg 3900
tcctcattga caaagagtca tgttcagttc aactggctag agccctaaga agccatatgt 3960
gggcaagact agctcgagga cggcctattt acggccttga ggtccctgat gtactagaat 4020
ctatgcgagg ccaccttatt cggcgtcatg agacatgtgt catctgcgag tgtggatcag 4080
tcaactacgg atggtttttt gtcccctcgg gttgccaact ggatgatatt gacaaggaaa 4140
catcatcctt gagagtccca tatattggtt ctaccactga tgagagaaca gacatgaagc 4200
tcgccttcgt aagagcccca agtcgatcct tgcgatctgc tgttagaata gcaacagtgt 4260
actcatgggc ttacggtgat gatgatagtt cttggaacga agcctggttg ttggcaaggc 4320
aaagggccaa tgtgagcctg gaggagctaa gggtgatcac tcccatctca acttcgacta 4380
atttagcgca taggttgagg gatcgtagca ctcaagtgaa atactcaggt acatcccttg 4440
tccgagtggc aaggtatacc acaatctcca acgacaatct ctcatttgtc atatcagata 4500
agaaggttga tactaacttt atataccaac aaggaatgct tctagggttg ggtgttttag 4560
aaacattgtt tcgactcgag aaagatactg gatcatctaa cacggtatta catcttcacg 4620
tcgaaacaga ttgttgcgtg atcccgatga tagatcatcc caggataccc agctcccgca 4680
agctagagct gagggcagag ctatgtacca acccattgat atatgataat gcacctttaa 4740
ttgacagaga tgcaacaagg ctatacaccc agagccatag gaggcacctt gtggaatttg 4800
ttacatggtc cacaccccaa ctatatcaca ttctagctaa gtccacagca ctatctatga 4860
ttgacctggt aacaaaattt gagaaggacc atatgaatga aatttcagct ctcatagggg 4920
atgacgatat caatagtttc ataactgagt ttctgcttat agagccaaga ttattcacta 4980
tctacttggg ccagtgtgca gccatcaatt gggcatttga tatacattat catagaccat 5040
cagggaaata tcagatgggt gagctgttgt cttcgttcct ttctagaatg agcaaaggag 5100
tgtttaaggt gcttgtcaat gctctaagcc acccaaagat ctacaagaaa ttctggcact 5160
gtggtattat agagcctatc catggtcctt cacttgatgc tcaaaacttg cacacaactg 5220
tgtgcaacat ggtttacaca tgctatatga cctacctcga cctgttgttg aatgaagagt 5280
tagaagagtt cacatttctt ttgtgtgaaa gcgacgagga tgtagtaccg gacagattcg 5340
acaacatcca ggcaaaacac ttgtgtgttc tagcagattt gtactgtcaa ccagggacct 5400
gcccaccgat tcgaggtcta agaccggtag agaaatgtgc agttctaacc gatcatatca 5460
aggcagaggc taggttatct ccagcagggt cttcgtggaa cataaatcca attattgtag 5520
accattactc atgctctctg acttatctcc ggcggggatc tatcaaacag ataagattga 5580
gagttgatcc gggattcatt tttgacgccc tcgctgaggt aaatgtcagt caaccaaaga 5640
tcggcagcaa caacatctca aatatgagca tcaaggattt cagacctcca cacgatgatg 5700
ttgcaaaact gctcaaagat atcaacacaa gcaagcacaa tcttcccatt tcagggggta 5760
gtctcgccaa ttatgaaatc catgctttcc gcaggatcgg gttaaactca tctgcttgct 5820
acaaagctgt tgagatatca acattaatta ggagatgcct tgagccaggg gaagacggct 5880
tgttcttggg tgaggggtcg ggttctatgt tgatcactta taaggagata ctaaaactaa 5940
acaagtgctt ctataatagt ggggtttccg ccaattctag atctggtcaa agggaattag 6000
caccctatcc ctccgaagtt ggccttgtcg aacacagaat gggagtaggt aatattgtca 6060
aagtgctctt taacgggagg cccgaagtca cgtgggtagg cagtatagat tgcttcaatt 6120
tcatagtcag taacatccct acctctagtg tggggtttat ccattcagat atagagacct 6180
tacctaacaa agatactata gagaagctag aggaattggc agccatctta tcgatggctc 6240
tgctccttgg caaaatagga tcaatactgg tgattaagct tatgccttta agcggggatt 6300
ttgttcaggg atttataagc tatgtagggt ctcattatag agaagtgaac cttgtttacc 6360
ctaggtacag caacttcata tctactgaat cttatttagt tatgacagat ctcaaagcta 6420
accggctaat aaatcctgaa aagattaagc agcagataat cgaatcatct gtgcggactt 6480
cacctggact tataggtcac atcctatcca ttaagcaact aagctgcata caagcaattg 6540
tgggaggcgc agttagtaga ggtgatatca accctactct gaaaaaactt acacctatag 6600
agcaggtgct gatcagttgc gggttgacaa ttaacggacc taaactgtgc aaagaattaa 6660
tccaccatga tgttgcctca gggcaagatg gattgcttaa ctctatactc atcctctaca 6720
gggagttggc aagattcaaa gacaaccaaa gaagtcaaca agggatgttc cacgcttacc 6780
ccgtattggt aagtagcagg caacgagaac ttgtatctag gatcacccgc aaattttggg 6840
ggcatattct tctttactcc gggaacagaa agttgataaa tcggtttatc cagaatctca 6900
aatccggtta tctaatacta gacttacacc agaatatctt cgttaagaat ctatctaagt 6960
cagagaaaca gattattatg acggggggtt taaaacgtga gtgggttttt aaggtaacag 7020
tcaaggagac caaagaatgg tataagttgg tcggatacag tgccctgatt aaggattaag 7080
aattccttaa ttaatgcggc cgcagcgatc gcactcgagc accaccacca ccccactgag 7140
atctccgatc ctctagagtc gatcgacctg caggcatgca agcttttgtt ccctttagtg 7200
agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 7260
tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 7320
ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 7380
aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 7440
tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 7500
gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 7560
cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 7620
gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 7680
aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 7740
ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7800
cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7860
ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7920
cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7980
agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 8040
gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 8100
gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 8160
tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 8220
agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 8280
agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 8340
atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 8400
cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 8460
actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 8520
aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 8580
cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 8640
ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 8700
cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 8760
ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8820
cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8880
ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 8940
tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 9000
ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 9060
aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 9120
gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 9180
gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 9240
ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 9300
catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 9360
atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 9420
taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 9480
cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 9540
cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 9600
tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac 9660
agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 9720
tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 9780
tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 9840
agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 9900
gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 9960
aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 10020
cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 10080
gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 10140
gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 10200
cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 10260
taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg 10320
actcactata 10330
<210> 36
<211> 2181
<212> PRT
<213> Measles virus
<400> 36
Met Asp Ser Leu Ser Val Asn Gln Ile Leu Tyr Pro Glu Val His Leu
1 5 10 15
Asp Ser Pro Ile Val Thr Asn Lys Ile Val Ala Ile Leu Glu Tyr Ala
20 25 30
Arg Val Pro His Ala Tyr Ser Leu Glu Asp Pro Thr Leu Cys Gln Asn
35 40 45
Ile Lys His Arg Leu Lys Asn Gly Phe Ser Asn Gln Met Ile Ile Asn
50 55 60
Asn Val Glu Val Gly Asn Val Ile Lys Ser Lys Leu Arg Ser Tyr Pro
65 70 75 80
Ala His Ser His Ile Pro Tyr Pro Asn Cys Asn Gln Asp Leu Phe Asn
85 90 95
Ile Glu Asp Lys Glu Ser Thr Arg Lys Ile Arg Glu Leu Leu Lys Lys
100 105 110
Gly Asn Ser Leu Tyr Ser Lys Val Ser Asp Lys Val Phe Gln Cys Leu
115 120 125
Arg Asp Thr Asn Ser Arg Leu Gly Leu Gly Ser Glu Leu Arg Glu Asp
130 135 140
Ile Lys Glu Lys Ile Ile Asn Leu Gly Val Tyr Met His Ser Ser Gln
145 150 155 160
Trp Phe Glu Pro Phe Leu Phe Trp Phe Thr Val Lys Thr Glu Met Arg
165 170 175
Ser Val Ile Lys Ser Gln Thr His Thr Cys His Arg Arg Arg His Thr
180 185 190
Pro Val Phe Phe Thr Gly Ser Ser Val Glu Leu Leu Ile Ser Arg Asp
195 200 205
Leu Val Ala Ile Ile Ser Lys Glu Ser Gln His Val Tyr Tyr Leu Thr
210 215 220
Phe Glu Leu Val Leu Met Tyr Cys Asp Val Ile Glu Gly Arg Leu Met
225 230 235 240
Thr Glu Thr Ala Met Thr Ile Asp Ala Arg Tyr Ala Glu Leu Leu Gly
245 250 255
Arg Val Arg Tyr Met Trp Lys Leu Ile Asp Gly Phe Phe Pro Ala Leu
260 265 270
Gly Asn Pro Thr Tyr Gln Ile Val Ala Met Leu Glu Pro Leu Ser Leu
275 280 285
Ala Tyr Leu Gln Leu Arg Asp Ile Thr Val Glu Leu Arg Gly Ala Phe
290 295 300
Leu Asn His Cys Phe Thr Glu Ile His Asp Val Leu Asp Gln Asn Gly
305 310 315 320
Phe Ser Asp Glu Gly Thr Tyr His Glu Leu Ile Glu Ala Leu Asp Tyr
325 330 335
Ile Phe Ile Thr Asp Asp Ile His Leu Thr Gly Glu Ile Phe Ser Phe
340 345 350
Phe Arg Ser Phe Gly His Pro Arg Leu Glu Ala Val Thr Ala Ala Glu
355 360 365
Asn Val Arg Lys Tyr Met Asn Gln Pro Lys Val Ile Val Tyr Glu Thr
370 375 380
Leu Met Lys Gly His Ala Ile Phe Cys Gly Ile Ile Ile Asn Gly Tyr
385 390 395 400
Arg Asp Arg His Gly Gly Ser Trp Pro Pro Leu Thr Leu Pro Leu His
405 410 415
Ala Ala Asp Thr Ile Arg Asn Ala Gln Ala Ser Gly Glu Gly Leu Thr
420 425 430
His Glu Gln Cys Val Asp Asn Trp Lys Ser Phe Ala Gly Val Arg Phe
435 440 445
Gly Cys Phe Met Pro Leu Ser Leu Asp Ser Asp Leu Thr Met Tyr Leu
450 455 460
Lys Asp Lys Ala Leu Ala Ala Leu Gln Arg Glu Trp Asp Ser Val Tyr
465 470 475 480
Pro Lys Glu Phe Leu Arg Tyr Asp Pro Pro Lys Gly Thr Gly Ser Arg
485 490 495
Arg Leu Val Asp Val Phe Leu Asn Asp Ser Ser Phe Asp Pro Tyr Asp
500 505 510
Met Ile Met Tyr Val Val Ser Gly Ala Tyr Leu His Asp Pro Glu Phe
515 520 525
Asn Leu Ser Tyr Ser Leu Lys Glu Lys Glu Ile Lys Glu Thr Gly Arg
530 535 540
Leu Phe Ala Lys Met Thr Tyr Lys Met Arg Ala Cys Gln Val Ile Ala
545 550 555 560
Glu Asn Leu Ile Ser Asn Gly Ile Gly Lys Tyr Phe Lys Asp Asn Gly
565 570 575
Met Ala Lys Asp Glu His Asp Leu Thr Lys Ala Leu His Thr Leu Ala
580 585 590
Val Ser Gly Val Pro Lys Asp Leu Lys Glu Ser His Arg Gly Gly Pro
595 600 605
Val Leu Lys Thr Tyr Ser Arg Ser Pro Val His Thr Ser Thr Arg Asn
610 615 620
Val Lys Ala Glu Lys Gly Phe Val Gly Phe Pro His Val Ile Arg Gln
625 630 635 640
Asn Gln Asp Thr Asp His Pro Glu Asn Ile Glu Thr Tyr Glu Thr Val
645 650 655
Ser Ala Phe Ile Thr Thr Asp Leu Lys Lys Tyr Cys Leu Asn Trp Arg
660 665 670
Tyr Glu Thr Ile Ser Leu Phe Ala Gln Arg Leu Asn Glu Ile Tyr Gly
675 680 685
Leu Pro Ser Phe Phe Gln Trp Leu His Lys Arg Leu Glu Thr Ser Val
690 695 700
Leu Tyr Val Ser Asp Pro His Cys Pro Pro Asp Leu Asp Ala His Val
705 710 715 720
Pro Leu Cys Lys Val Pro Asn Asp Gln Ile Phe Ile Lys Tyr Pro Met
725 730 735
Gly Gly Ile Glu Gly Tyr Cys Gln Lys Leu Trp Thr Ile Ser Thr Ile
740 745 750
Pro Tyr Leu Tyr Leu Ala Ala Tyr Glu Ser Arg Val Arg Ile Ala Ser
755 760 765
Leu Val Gln Gly Asp Asn Gln Thr Ile Ala Val Thr Lys Arg Val Pro
770 775 780
Ser Thr Trp His Tyr Asn Leu Lys Lys Arg Glu Ala Ala Arg Val Thr
785 790 795 800
Arg Asp Tyr Phe Val Ile Leu Arg Gln Arg Leu His Asp Ile Gly His
805 810 815
His Leu Lys Ala Asn Glu Thr Ile Val Ser Ser His Phe Phe Val Tyr
820 825 830
Ser Lys Gly Ile Tyr Tyr Asp Gly Leu Leu Val Ser Gln Ser Leu Lys
835 840 845
Ser Ile Ala Arg Cys Val Phe Trp Ser Glu Thr Ile Val Asp Glu Thr
850 855 860
Arg Ala Ala Cys Ser Asn Ile Ala Thr Thr Met Ala Lys Ser Ile Glu
865 870 875 880
Arg Gly Tyr Asp Arg Tyr Leu Ala Tyr Ser Leu Asn Val Leu Lys Val
885 890 895
Ile Gln Gln Ile Leu Ile Ser Leu Gly Phe Thr Ile Asn Ser Thr Met
900 905 910
Thr Arg Asp Val Val Ile Pro Leu Leu Thr Asn Asn Asp Leu Leu Ile
915 920 925
Arg Met Ala Leu Leu Pro Ala Pro Ile Gly Gly Met Asn Tyr Leu Asn
930 935 940
Met Ser Arg Leu Phe Val Arg Asn Ile Gly Asp Pro Val Thr Ser Ser
945 950 955 960
Ile Ala Asp Leu Lys Arg Met Ile Leu Ala Ser Leu Met Pro Glu Glu
965 970 975
Thr Leu His Gln Val Met Thr Gln Gln Pro Gly Asp Ser Ser Phe Leu
980 985 990
Asp Trp Ala Ser Asp Pro Tyr Ser Ala Asn Leu Val Cys Val Gln Ser
995 1000 1005
Ile Thr Arg Leu Leu Lys Asn Ile Thr Ala Arg Phe Val Leu Ile
1010 1015 1020
His Ser Pro Asn Pro Met Leu Lys Gly Leu Phe His Asp Asp Ser
1025 1030 1035
Lys Glu Glu Asp Glu Arg Leu Ala Ala Phe Leu Met Asp Arg His
1040 1045 1050
Ile Ile Val Pro Arg Ala Ala His Glu Ile Leu Asp His Ser Val
1055 1060 1065
Thr Gly Ala Arg Glu Ser Ile Ala Gly Met Leu Asp Thr Thr Lys
1070 1075 1080
Gly Leu Ile Arg Ala Ser Met Arg Lys Gly Gly Leu Thr Ser Arg
1085 1090 1095
Val Ile Thr Arg Leu Ser Asn Tyr Asp Tyr Glu Gln Phe Arg Ala
1100 1105 1110
Gly Met Val Leu Leu Thr Gly Arg Lys Arg Asn Val Leu Ile Asp
1115 1120 1125
Lys Glu Ser Cys Ser Val Gln Leu Ala Arg Ala Leu Arg Ser His
1130 1135 1140
Met Trp Ala Arg Leu Ala Arg Gly Arg Pro Ile Tyr Gly Leu Glu
1145 1150 1155
Val Pro Asp Val Leu Glu Ser Met Arg Gly His Leu Ile Arg Arg
1160 1165 1170
His Glu Thr Cys Val Ile Cys Glu Cys Gly Ser Val Asn Tyr Gly
1175 1180 1185
Trp Phe Phe Val Pro Ser Gly Cys Gln Leu Asp Asp Ile Asp Lys
1190 1195 1200
Glu Thr Ser Ser Leu Arg Val Pro Tyr Ile Gly Ser Thr Thr Asp
1205 1210 1215
Glu Arg Thr Asp Met Lys Leu Ala Phe Val Arg Ala Pro Ser Arg
1220 1225 1230
Ser Leu Arg Ser Ala Val Arg Ile Ala Thr Val Tyr Ser Trp Ala
1235 1240 1245
Tyr Gly Asp Asp Asp Ser Ser Trp Asn Glu Ala Trp Leu Leu Ala
1250 1255 1260
Arg Gln Arg Ala Asn Val Ser Leu Glu Glu Leu Arg Val Ile Thr
1265 1270 1275
Pro Ile Ser Thr Ser Thr Asn Leu Ala His Arg Leu Arg Asp Arg
1280 1285 1290
Ser Thr Gln Val Lys Tyr Ser Gly Thr Ser Leu Val Arg Val Ala
1295 1300 1305
Arg Tyr Thr Thr Ile Ser Asn Asp Asn Leu Ser Phe Val Ile Ser
1310 1315 1320
Asp Lys Lys Val Asp Thr Asn Phe Ile Tyr Gln Gln Gly Met Leu
1325 1330 1335
Leu Gly Leu Gly Val Leu Glu Thr Leu Phe Arg Leu Glu Lys Asp
1340 1345 1350
Thr Gly Ser Ser Asn Thr Val Leu His Leu His Val Glu Thr Asp
1355 1360 1365
Cys Cys Val Ile Pro Met Ile Asp His Pro Arg Ile Pro Ser Ser
1370 1375 1380
Arg Lys Leu Glu Leu Arg Ala Glu Leu Cys Thr Asn Pro Leu Ile
1385 1390 1395
Tyr Asp Asn Ala Pro Leu Ile Asp Arg Asp Ala Thr Arg Leu Tyr
1400 1405 1410
Thr Gln Ser His Arg Arg His Leu Val Glu Phe Val Thr Trp Ser
1415 1420 1425
Thr Pro Gln Leu Tyr His Ile Leu Ala Lys Ser Thr Ala Leu Ser
1430 1435 1440
Met Ile Asp Leu Val Thr Lys Phe Glu Lys Asp His Met Asn Glu
1445 1450 1455
Ile Ser Ala Leu Ile Gly Asp Asp Asp Ile Asn Ser Phe Ile Thr
1460 1465 1470
Glu Phe Leu Leu Ile Glu Pro Arg Leu Phe Thr Ile Tyr Leu Gly
1475 1480 1485
Gln Cys Ala Ala Ile Asn Trp Ala Phe Asp Val His Tyr His Arg
1490 1495 1500
Pro Ser Gly Lys Tyr Gln Met Gly Glu Leu Leu Ser Ser Phe Leu
1505 1510 1515
Ser Arg Met Ser Lys Gly Val Phe Lys Val Leu Val Asn Ala Leu
1520 1525 1530
Ser His Pro Lys Ile Tyr Lys Lys Phe Trp His Cys Gly Ile Ile
1535 1540 1545
Glu Pro Ile His Gly Pro Ser Leu Asp Ala Gln Asn Leu His Thr
1550 1555 1560
Thr Val Cys Asn Met Val Tyr Thr Cys Tyr Met Thr Tyr Leu Asp
1565 1570 1575
Leu Leu Leu Asn Glu Glu Leu Glu Glu Phe Thr Phe Leu Leu Cys
1580 1585 1590
Glu Ser Asp Glu Asp Val Val Pro Asp Arg Phe Asp Asn Ile Gln
1595 1600 1605
Ala Lys His Leu Cys Val Leu Ala Asp Leu Tyr Cys Gln Pro Gly
1610 1615 1620
Thr Cys Pro Pro Ile Arg Gly Leu Arg Pro Val Glu Lys Cys Ala
1625 1630 1635
Val Leu Thr Asp His Ile Lys Ala Glu Ala Arg Leu Ser Pro Ala
1640 1645 1650
Gly Ser Ser Trp Asn Ile Asn Pro Ile Ile Val Asp His Tyr Ser
1655 1660 1665
Cys Ser Leu Thr Tyr Leu Arg Arg Gly Ser Ile Lys Gln Ile Arg
1670 1675 1680
Leu Arg Val Asp Pro Gly Phe Ile Phe Asp Ala Leu Ala Glu Val
1685 1690 1695
Asn Val Ser Gln Pro Lys Val Gly Ser Ile Ser Asn Met Ser Ile
1700 1705 1710
Lys Asp Phe Arg Pro Pro His Asp Asp Val Ala Lys Leu Leu Lys
1715 1720 1725
Asp Ile Asn Thr Ser Lys His Asn Leu Pro Ile Ser Gly Gly Ser
1730 1735 1740
Leu Ala Asn Tyr Glu Ile His Ala Phe Arg Arg Ile Gly Leu Asn
1745 1750 1755
Ser Ser Ala Cys Tyr Lys Ala Val Glu Ile Ser Thr Leu Ile Arg
1760 1765 1770
Arg Cys Leu Glu Pro Gly Glu Asp Gly Leu Phe Leu Gly Glu Gly
1775 1780 1785
Ser Gly Ser Met Leu Ile Thr Tyr Lys Glu Ile Leu Lys Leu Asn
1790 1795 1800
Lys Cys Phe Tyr Asn Ser Gly Val Ser Ala Asn Ser Arg Ser Gly
1805 1810 1815
Gln Arg Glu Leu Ala Pro Tyr Pro Ser Glu Val Gly Leu Val Glu
1820 1825 1830
His Arg Met Gly Val Gly Asn Ile Val Lys Val Leu Phe Asn Gly
1835 1840 1845
Arg Pro Glu Val Thr Trp Val Gly Ser Ile Asp Cys Phe Asn Phe
1850 1855 1860
Ile Val Ser Asn Ile Pro Thr Ser Ser Val Gly Phe Ile His Ser
1865 1870 1875
Asp Ile Glu Thr Leu Pro Asn Lys Asp Thr Ile Glu Lys Leu Glu
1880 1885 1890
Glu Leu Ala Ala Ile Leu Ser Met Ala Leu Leu Leu Gly Lys Ile
1895 1900 1905
Gly Ser Ile Leu Val Ile Lys Leu Met Pro Phe Ser Gly Asp Phe
1910 1915 1920
Val Gln Gly Phe Ile Ser Tyr Val Gly Ser His Tyr Arg Glu Val
1925 1930 1935
Asn Leu Val Tyr Pro Arg Tyr Ser Asn Phe Ile Ser Thr Glu Ser
1940 1945 1950
Tyr Leu Val Met Thr Asp Leu Lys Ala Asn Arg Leu Met Asn Pro
1955 1960 1965
Glu Lys Ile Lys Gln Gln Ile Ile Glu Ser Ser Val Arg Thr Ser
1970 1975 1980
Pro Gly Leu Ile Gly His Ile Leu Ser Ile Lys Gln Leu Ser Cys
1985 1990 1995
Ile Gln Ala Ile Val Gly Gly Ala Val Ser Arg Gly Asp Ile Asn
2000 2005 2010
Pro Ile Leu Lys Lys Leu Thr Pro Ile Glu Gln Val Leu Ile Ser
2015 2020 2025
Cys Gly Leu Ala Ile Asn Gly Pro Lys Leu Cys Lys Glu Leu Ile
2030 2035 2040
His His Asp Val Ala Ser Gly Gln Asp Gly Leu Leu Asn Ser Ile
2045 2050 2055
Leu Ile Leu Tyr Arg Glu Leu Ala Arg Phe Lys Asp Asn Gln Arg
2060 2065 2070
Ser Gln Gln Gly Met Phe His Ala Tyr Pro Val Leu Val Ser Ser
2075 2080 2085
Arg Gln Arg Glu Leu Val Ser Arg Ile Thr Arg Lys Phe Trp Gly
2090 2095 2100
His Ile Leu Leu Tyr Ser Gly Asn Arg Lys Leu Ile Asn Arg Phe
2105 2110 2115
Ile Gln Asn Leu Lys Ser Gly Tyr Leu Val Leu Asp Leu His Gln
2120 2125 2130
Asn Ile Phe Val Lys Asn Leu Ser Lys Ser Glu Lys Gln Ile Ile
2135 2140 2145
Met Thr Gly Gly Leu Lys Arg Glu Trp Val Phe Lys Val Thr Val
2150 2155 2160
Lys Glu Thr Lys Glu Trp Tyr Lys Leu Val Gly Tyr Ser Ala Leu
2165 2170 2175
Ile Lys Asp
2180
<210> 37
<211> 2425
<212> PRT
<213> Artificial Sequence
<220>
<223> Ln-EGFP-Lc
<400> 37
Met Asp Ser Leu Ser Val Asn Gln Ile Leu Tyr Pro Glu Val His Leu
1 5 10 15
Asp Ser Pro Ile Val Thr Asn Lys Ile Val Ala Ile Leu Glu Tyr Ala
20 25 30
Arg Val Pro His Ala Tyr Ser Leu Glu Asp Pro Thr Leu Cys Gln Asn
35 40 45
Ile Lys His Arg Leu Lys Asn Gly Phe Ser Asn Gln Met Ile Ile Asn
50 55 60
Asn Val Glu Val Gly Asn Val Ile Lys Ser Lys Leu Arg Ser Tyr Pro
65 70 75 80
Ala His Ser His Ile Pro Tyr Pro Asn Cys Asn Gln Asp Leu Phe Asn
85 90 95
Ile Glu Asp Lys Glu Ser Thr Arg Lys Ile Arg Glu Leu Leu Lys Lys
100 105 110
Gly Asn Ser Leu Tyr Ser Lys Val Ser Asp Lys Val Phe Gln Cys Leu
115 120 125
Arg Asp Thr Asn Ser Arg Leu Gly Leu Gly Ser Glu Leu Arg Glu Asp
130 135 140
Ile Lys Glu Lys Ile Ile Asn Leu Gly Val Tyr Met His Ser Ser Gln
145 150 155 160
Trp Phe Glu Pro Phe Leu Phe Trp Phe Thr Val Lys Thr Glu Met Arg
165 170 175
Ser Val Ile Lys Ser Gln Thr His Thr Cys His Arg Arg Arg His Thr
180 185 190
Pro Val Phe Phe Thr Gly Ser Ser Val Glu Leu Leu Ile Ser Arg Asp
195 200 205
Leu Val Ala Ile Ile Ser Lys Glu Ser Gln His Val Tyr Tyr Leu Thr
210 215 220
Phe Glu Leu Val Leu Met Tyr Cys Asp Val Ile Glu Gly Arg Leu Met
225 230 235 240
Thr Glu Thr Ala Met Thr Ile Asp Ala Arg Tyr Ala Glu Leu Leu Gly
245 250 255
Arg Val Arg Tyr Met Trp Lys Leu Ile Asp Gly Phe Phe Pro Ala Leu
260 265 270
Gly Asn Pro Thr Tyr Gln Ile Val Ala Met Leu Glu Pro Leu Ser Leu
275 280 285
Ala Tyr Leu Gln Leu Arg Asp Ile Thr Val Glu Leu Arg Gly Ala Phe
290 295 300
Leu Asn His Cys Phe Thr Glu Ile His Asp Val Leu Asp Gln Asn Gly
305 310 315 320
Phe Ser Asp Glu Gly Thr Tyr His Glu Leu Ile Glu Ala Leu Asp Tyr
325 330 335
Ile Phe Ile Thr Asp Asp Ile His Leu Thr Gly Glu Ile Phe Ser Phe
340 345 350
Phe Arg Ser Phe Gly His Pro Arg Leu Glu Ala Val Thr Ala Ala Glu
355 360 365
Asn Val Arg Lys Tyr Met Asn Gln Pro Lys Val Ile Val Tyr Glu Thr
370 375 380
Leu Met Lys Gly His Ala Ile Phe Cys Gly Ile Ile Ile Asn Gly Tyr
385 390 395 400
Arg Asp Arg His Gly Gly Ser Trp Pro Pro Leu Thr Leu Pro Leu His
405 410 415
Ala Ala Asp Thr Ile Arg Asn Ala Gln Ala Ser Gly Glu Gly Leu Thr
420 425 430
His Glu Gln Cys Val Asp Asn Trp Lys Ser Phe Ala Gly Val Arg Phe
435 440 445
Gly Cys Phe Met Pro Leu Ser Leu Asp Ser Asp Leu Thr Met Tyr Leu
450 455 460
Lys Asp Lys Ala Leu Ala Ala Leu Gln Arg Glu Trp Asp Ser Val Tyr
465 470 475 480
Pro Lys Glu Phe Leu Arg Tyr Asp Pro Pro Lys Gly Thr Gly Ser Arg
485 490 495
Arg Leu Val Asp Val Phe Leu Asn Asp Ser Ser Phe Asp Pro Tyr Asp
500 505 510
Met Ile Met Tyr Val Val Ser Gly Ala Tyr Leu His Asp Pro Glu Phe
515 520 525
Asn Leu Ser Tyr Ser Leu Lys Glu Lys Glu Ile Lys Glu Thr Gly Arg
530 535 540
Leu Phe Ala Lys Met Thr Tyr Lys Met Arg Ala Cys Gln Val Ile Ala
545 550 555 560
Glu Asn Leu Ile Ser Asn Gly Ile Gly Lys Tyr Phe Lys Asp Asn Gly
565 570 575
Met Ala Lys Asp Glu His Asp Leu Thr Lys Ala Leu His Thr Leu Ala
580 585 590
Val Ser Gly Val Pro Lys Asp Leu Lys Glu Ser His Arg Gly Gly Pro
595 600 605
Val Leu Lys Thr Tyr Ser Arg Ser Pro Val His Thr Ser Thr Arg Asn
610 615 620
Val Lys Ala Glu Lys Gly Phe Val Gly Phe Pro His Val Ile Arg Gln
625 630 635 640
Asn Gln Asp Thr Asp His Pro Glu Asn Ile Glu Thr Tyr Glu Thr Val
645 650 655
Ser Ala Phe Ile Thr Thr Asp Leu Lys Lys Tyr Cys Leu Asn Trp Arg
660 665 670
Tyr Glu Thr Ile Ser Leu Phe Ala Gln Arg Leu Asn Glu Ile Tyr Gly
675 680 685
Leu Pro Ser Phe Phe Gln Trp Leu His Lys Arg Leu Glu Thr Ser Val
690 695 700
Leu Tyr Val Ser Asp Pro His Cys Pro Pro Asp Leu Asp Ala His Val
705 710 715 720
Pro Leu Cys Lys Val Pro Asn Asp Gln Ile Phe Ile Lys Tyr Pro Met
725 730 735
Gly Gly Ile Glu Gly Tyr Cys Gln Lys Leu Trp Thr Ile Ser Thr Ile
740 745 750
Pro Tyr Leu Tyr Leu Ala Ala Tyr Glu Ser Arg Val Arg Ile Ala Ser
755 760 765
Leu Val Gln Gly Asp Asn Gln Thr Ile Ala Val Thr Lys Arg Val Pro
770 775 780
Ser Thr Trp His Tyr Asn Leu Lys Lys Arg Glu Ala Ala Arg Val Thr
785 790 795 800
Arg Asp Tyr Phe Val Ile Leu Arg Gln Arg Leu His Asp Ile Gly His
805 810 815
His Leu Lys Ala Asn Glu Thr Ile Val Ser Ser His Phe Phe Val Tyr
820 825 830
Ser Lys Gly Ile Tyr Tyr Asp Gly Leu Leu Val Ser Gln Ser Leu Lys
835 840 845
Ser Ile Ala Arg Cys Val Phe Trp Ser Glu Thr Ile Val Asp Glu Thr
850 855 860
Arg Ala Ala Cys Ser Asn Ile Ala Thr Thr Met Ala Lys Ser Ile Glu
865 870 875 880
Arg Gly Tyr Asp Arg Tyr Leu Ala Tyr Ser Leu Asn Val Leu Lys Val
885 890 895
Ile Gln Gln Ile Leu Ile Ser Leu Gly Phe Thr Ile Asn Ser Thr Met
900 905 910
Thr Arg Asp Val Val Ile Pro Leu Leu Thr Asn Asn Asp Leu Leu Ile
915 920 925
Arg Met Ala Leu Leu Pro Ala Pro Ile Gly Gly Met Asn Tyr Leu Asn
930 935 940
Met Ser Arg Leu Phe Val Arg Asn Ile Gly Asp Pro Val Thr Ser Ser
945 950 955 960
Ile Ala Asp Leu Lys Arg Met Ile Leu Ala Ser Leu Met Pro Glu Glu
965 970 975
Thr Leu His Gln Val Met Thr Gln Gln Pro Gly Asp Ser Ser Phe Leu
980 985 990
Asp Trp Ala Ser Asp Pro Tyr Ser Ala Asn Leu Val Cys Val Gln Ser
995 1000 1005
Ile Thr Arg Leu Leu Lys Asn Ile Thr Ala Arg Phe Val Leu Ile
1010 1015 1020
His Ser Pro Asn Pro Met Leu Lys Gly Leu Phe His Asp Asp Ser
1025 1030 1035
Lys Glu Glu Asp Glu Arg Leu Ala Ala Phe Leu Met Asp Arg His
1040 1045 1050
Ile Ile Val Pro Arg Ala Ala His Glu Ile Leu Asp His Ser Val
1055 1060 1065
Thr Gly Ala Arg Glu Ser Ile Ala Gly Met Leu Asp Thr Thr Lys
1070 1075 1080
Gly Leu Ile Arg Ala Ser Met Arg Lys Gly Gly Leu Thr Ser Arg
1085 1090 1095
Val Ile Thr Arg Leu Ser Asn Tyr Asp Tyr Glu Gln Phe Arg Ala
1100 1105 1110
Gly Met Val Leu Leu Thr Gly Arg Lys Arg Asn Val Leu Ile Asp
1115 1120 1125
Lys Glu Ser Cys Ser Val Gln Leu Ala Arg Ala Leu Arg Ser His
1130 1135 1140
Met Trp Ala Arg Leu Ala Arg Gly Arg Pro Ile Tyr Gly Leu Glu
1145 1150 1155
Val Pro Asp Val Leu Glu Ser Met Arg Gly His Leu Ile Arg Arg
1160 1165 1170
His Glu Thr Cys Val Ile Cys Glu Cys Gly Ser Val Asn Tyr Gly
1175 1180 1185
Trp Phe Phe Val Pro Ser Gly Cys Gln Leu Asp Asp Ile Asp Lys
1190 1195 1200
Glu Thr Ser Ser Leu Arg Val Pro Tyr Ile Gly Ser Thr Thr Asp
1205 1210 1215
Glu Arg Thr Asp Met Lys Leu Ala Phe Val Arg Ala Pro Ser Arg
1220 1225 1230
Ser Leu Arg Ser Ala Val Arg Ile Ala Thr Val Tyr Ser Trp Ala
1235 1240 1245
Tyr Gly Asp Asp Asp Ser Ser Trp Asn Glu Ala Trp Leu Leu Ala
1250 1255 1260
Arg Gln Arg Ala Asn Val Ser Leu Glu Glu Leu Arg Val Ile Thr
1265 1270 1275
Pro Ile Ser Thr Ser Thr Asn Leu Ala His Arg Leu Arg Asp Arg
1280 1285 1290
Ser Thr Gln Val Lys Tyr Ser Gly Thr Ser Leu Val Arg Val Ala
1295 1300 1305
Arg Tyr Thr Thr Ile Ser Asn Asp Asn Leu Ser Phe Val Ile Ser
1310 1315 1320
Asp Lys Lys Val Asp Thr Asn Phe Ile Tyr Gln Gln Gly Met Leu
1325 1330 1335
Leu Gly Leu Gly Val Leu Glu Thr Leu Phe Arg Leu Glu Lys Asp
1340 1345 1350
Thr Gly Ser Ser Asn Thr Val Leu His Leu His Val Glu Thr Asp
1355 1360 1365
Cys Cys Val Ile Pro Met Ile Asp His Pro Arg Ile Pro Ser Ser
1370 1375 1380
Arg Lys Leu Glu Leu Arg Ala Glu Leu Cys Thr Asn Pro Leu Ile
1385 1390 1395
Tyr Asp Asn Ala Pro Leu Ile Asp Arg Asp Ala Thr Arg Leu Tyr
1400 1405 1410
Thr Gln Ser His Arg Arg His Leu Val Glu Phe Val Thr Trp Ser
1415 1420 1425
Thr Pro Gln Leu Tyr His Ile Leu Ala Lys Ser Thr Ala Leu Ser
1430 1435 1440
Met Ile Asp Leu Val Thr Lys Phe Glu Lys Asp His Met Asn Glu
1445 1450 1455
Ile Ser Ala Leu Ile Gly Asp Asp Asp Ile Asn Ser Phe Ile Thr
1460 1465 1470
Glu Phe Leu Leu Ile Glu Pro Arg Leu Phe Thr Ile Tyr Leu Gly
1475 1480 1485
Gln Cys Ala Ala Ile Asn Trp Ala Phe Asp Val His Tyr His Arg
1490 1495 1500
Pro Ser Gly Lys Tyr Gln Met Gly Glu Leu Leu Ser Ser Phe Leu
1505 1510 1515
Ser Arg Met Ser Lys Gly Val Phe Lys Val Leu Val Asn Ala Leu
1520 1525 1530
Ser His Pro Lys Ile Tyr Lys Lys Phe Trp His Cys Gly Ile Ile
1535 1540 1545
Glu Pro Ile His Gly Pro Ser Leu Asp Ala Gln Asn Leu His Thr
1550 1555 1560
Thr Val Cys Asn Met Val Tyr Thr Cys Tyr Met Thr Tyr Leu Asp
1565 1570 1575
Leu Leu Leu Asn Glu Glu Leu Glu Glu Phe Thr Phe Leu Leu Cys
1580 1585 1590
Glu Ser Asp Glu Asp Val Val Pro Asp Arg Phe Asp Asn Ile Gln
1595 1600 1605
Ala Lys His Leu Cys Val Leu Ala Asp Leu Tyr Cys Gln Pro Gly
1610 1615 1620
Thr Cys Pro Pro Ile Arg Gly Leu Arg Pro Val Glu Lys Cys Ala
1625 1630 1635
Val Leu Thr Asp His Ile Lys Ala Glu Ala Arg Leu Ser Pro Ala
1640 1645 1650
Gly Ser Ser Trp Asn Ile Asn Pro Ile Ile Val Asp His Tyr Ser
1655 1660 1665
Cys Ser Leu Thr Tyr Leu Arg Arg Gly Ser Ile Lys Gln Ile Arg
1670 1675 1680
Leu Arg Val Asp Pro Gly Phe Ile Phe Asp Ala Leu Ala Glu Val
1685 1690 1695
Asn Val Ser Gln Pro Lys Val Gly Ser Asp Val Met Val Ser Lys
1700 1705 1710
Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu
1715 1720 1725
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly
1730 1735 1740
Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys
1745 1750 1755
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr
1760 1765 1770
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met
1775 1780 1785
Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val
1790 1795 1800
Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr
1805 1810 1815
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile
1820 1825 1830
Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
1835 1840 1845
His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met
1850 1855 1860
Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg
1865 1870 1875
His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln
1880 1885 1890
Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
1895 1900 1905
His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu
1910 1915 1920
Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly
1925 1930 1935
Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly Asp Val Ile Ser
1940 1945 1950
Asn Met Ser Ile Lys Asp Phe Arg Pro Pro His Asp Asp Val Ala
1955 1960 1965
Lys Leu Leu Lys Asp Ile Asn Thr Ser Lys His Asn Leu Pro Ile
1970 1975 1980
Ser Gly Gly Ser Leu Ala Asn Tyr Glu Ile His Ala Phe Arg Arg
1985 1990 1995
Ile Gly Leu Asn Ser Ser Ala Cys Tyr Lys Ala Val Glu Ile Ser
2000 2005 2010
Thr Leu Ile Arg Arg Cys Leu Glu Pro Gly Glu Asp Gly Leu Phe
2015 2020 2025
Leu Gly Glu Gly Ser Gly Ser Met Leu Ile Thr Tyr Lys Glu Ile
2030 2035 2040
Leu Lys Leu Asn Lys Cys Phe Tyr Asn Ser Gly Val Ser Ala Asn
2045 2050 2055
Ser Arg Ser Gly Gln Arg Glu Leu Ala Pro Tyr Pro Ser Glu Val
2060 2065 2070
Gly Leu Val Glu His Arg Met Gly Val Gly Asn Ile Val Lys Val
2075 2080 2085
Leu Phe Asn Gly Arg Pro Glu Val Thr Trp Val Gly Ser Ile Asp
2090 2095 2100
Cys Phe Asn Phe Ile Val Ser Asn Ile Pro Thr Ser Ser Val Gly
2105 2110 2115
Phe Ile His Ser Asp Ile Glu Thr Leu Pro Asn Lys Asp Thr Ile
2120 2125 2130
Glu Lys Leu Glu Glu Leu Ala Ala Ile Leu Ser Met Ala Leu Leu
2135 2140 2145
Leu Gly Lys Ile Gly Ser Ile Leu Val Ile Lys Leu Met Pro Phe
2150 2155 2160
Ser Gly Asp Phe Val Gln Gly Phe Ile Ser Tyr Val Gly Ser His
2165 2170 2175
Tyr Arg Glu Val Asn Leu Val Tyr Pro Arg Tyr Ser Asn Phe Ile
2180 2185 2190
Ser Thr Glu Ser Tyr Leu Val Met Thr Asp Leu Lys Ala Asn Arg
2195 2200 2205
Leu Met Asn Pro Glu Lys Ile Lys Gln Gln Ile Ile Glu Ser Ser
2210 2215 2220
Val Arg Thr Ser Pro Gly Leu Ile Gly His Ile Leu Ser Ile Lys
2225 2230 2235
Gln Leu Ser Cys Ile Gln Ala Ile Val Gly Gly Ala Val Ser Arg
2240 2245 2250
Gly Asp Ile Asn Pro Ile Leu Lys Lys Leu Thr Pro Ile Glu Gln
2255 2260 2265
Val Leu Ile Ser Cys Gly Leu Ala Ile Asn Gly Pro Lys Leu Cys
2270 2275 2280
Lys Glu Leu Ile His His Asp Val Ala Ser Gly Gln Asp Gly Leu
2285 2290 2295
Leu Asn Ser Ile Leu Ile Leu Tyr Arg Glu Leu Ala Arg Phe Lys
2300 2305 2310
Asp Asn Gln Arg Ser Gln Gln Gly Met Phe His Ala Tyr Pro Val
2315 2320 2325
Leu Val Ser Ser Arg Gln Arg Glu Leu Val Ser Arg Ile Thr Arg
2330 2335 2340
Lys Phe Trp Gly His Ile Leu Leu Tyr Ser Gly Asn Arg Lys Leu
2345 2350 2355
Ile Asn Arg Phe Ile Gln Asn Leu Lys Ser Gly Tyr Leu Val Leu
2360 2365 2370
Asp Leu His Gln Asn Ile Phe Val Lys Asn Leu Ser Lys Ser Glu
2375 2380 2385
Lys Gln Ile Ile Met Thr Gly Gly Leu Lys Arg Glu Trp Val Phe
2390 2395 2400
Lys Val Thr Val Lys Glu Thr Lys Glu Trp Tyr Lys Leu Val Gly
2405 2410 2415
Tyr Ser Ala Leu Ile Lys Asp
2420 2425
<210> 38
<211> 2661
<212> PRT
<213> Artificial Sequence
<220>
<223> Ln-DPK?DPN-Lc
<400> 38
Met Asp Ser Leu Ser Val Asn Gln Ile Leu Tyr Pro Glu Val His Leu
1 5 10 15
Asp Ser Pro Ile Val Thr Asn Lys Ile Val Ala Ile Leu Glu Tyr Ala
20 25 30
Arg Val Pro His Ala Tyr Ser Leu Glu Asp Pro Thr Leu Cys Gln Asn
35 40 45
Ile Lys His Arg Leu Lys Asn Gly Phe Ser Asn Gln Met Ile Ile Asn
50 55 60
Asn Val Glu Val Gly Asn Val Ile Lys Ser Lys Leu Arg Ser Tyr Pro
65 70 75 80
Ala His Ser His Ile Pro Tyr Pro Asn Cys Asn Gln Asp Leu Phe Asn
85 90 95
Ile Glu Asp Lys Glu Ser Thr Arg Lys Ile Arg Glu Leu Leu Lys Lys
100 105 110
Gly Asn Ser Leu Tyr Ser Lys Val Ser Asp Lys Val Phe Gln Cys Leu
115 120 125
Arg Asp Thr Asn Ser Arg Leu Gly Leu Gly Ser Glu Leu Arg Glu Asp
130 135 140
Ile Lys Glu Lys Ile Ile Asn Leu Gly Val Tyr Met His Ser Ser Gln
145 150 155 160
Trp Phe Glu Pro Phe Leu Phe Trp Phe Thr Val Lys Thr Glu Met Arg
165 170 175
Ser Val Ile Lys Ser Gln Thr His Thr Cys His Arg Arg Arg His Thr
180 185 190
Pro Val Phe Phe Thr Gly Ser Ser Val Glu Leu Leu Ile Ser Arg Asp
195 200 205
Leu Val Ala Ile Ile Ser Lys Glu Ser Gln His Val Tyr Tyr Leu Thr
210 215 220
Phe Glu Leu Val Leu Met Tyr Cys Asp Val Ile Glu Gly Arg Leu Met
225 230 235 240
Thr Glu Thr Ala Met Thr Ile Asp Ala Arg Tyr Ala Glu Leu Leu Gly
245 250 255
Arg Val Arg Tyr Met Trp Lys Leu Ile Asp Gly Phe Phe Pro Ala Leu
260 265 270
Gly Asn Pro Thr Tyr Gln Ile Val Ala Met Leu Glu Pro Leu Ser Leu
275 280 285
Ala Tyr Leu Gln Leu Arg Asp Ile Thr Val Glu Leu Arg Gly Ala Phe
290 295 300
Leu Asn His Cys Phe Thr Glu Ile His Asp Val Leu Asp Gln Asn Gly
305 310 315 320
Phe Ser Asp Glu Gly Thr Tyr His Glu Leu Ile Glu Ala Leu Asp Tyr
325 330 335
Ile Phe Ile Thr Asp Asp Ile His Leu Thr Gly Glu Ile Phe Ser Phe
340 345 350
Phe Arg Ser Phe Gly His Pro Arg Leu Glu Ala Val Thr Ala Ala Glu
355 360 365
Asn Val Arg Lys Tyr Met Asn Gln Pro Lys Val Ile Val Tyr Glu Thr
370 375 380
Leu Met Lys Gly His Ala Ile Phe Cys Gly Ile Ile Ile Asn Gly Tyr
385 390 395 400
Arg Asp Arg His Gly Gly Ser Trp Pro Pro Leu Thr Leu Pro Leu His
405 410 415
Ala Ala Asp Thr Ile Arg Asn Ala Gln Ala Ser Gly Glu Gly Leu Thr
420 425 430
His Glu Gln Cys Val Asp Asn Trp Lys Ser Phe Ala Gly Val Arg Phe
435 440 445
Gly Cys Phe Met Pro Leu Ser Leu Asp Ser Asp Leu Thr Met Tyr Leu
450 455 460
Lys Asp Lys Ala Leu Ala Ala Leu Gln Arg Glu Trp Asp Ser Val Tyr
465 470 475 480
Pro Lys Glu Phe Leu Arg Tyr Asp Pro Pro Lys Gly Thr Gly Ser Arg
485 490 495
Arg Leu Val Asp Val Phe Leu Asn Asp Ser Ser Phe Asp Pro Tyr Asp
500 505 510
Met Ile Met Tyr Val Val Ser Gly Ala Tyr Leu His Asp Pro Glu Phe
515 520 525
Asn Leu Ser Tyr Ser Leu Lys Glu Lys Glu Ile Lys Glu Thr Gly Arg
530 535 540
Leu Phe Ala Lys Met Thr Tyr Lys Met Arg Ala Cys Gln Val Ile Ala
545 550 555 560
Glu Asn Leu Ile Ser Asn Gly Ile Gly Lys Tyr Phe Lys Asp Asn Gly
565 570 575
Met Ala Lys Asp Glu His Asp Leu Thr Lys Ala Leu His Thr Leu Ala
580 585 590
Val Ser Gly Val Pro Lys Asp Leu Lys Glu Ser His Arg Gly Gly Pro
595 600 605
Val Leu Lys Thr Tyr Ser Arg Ser Pro Val His Thr Ser Thr Arg Asn
610 615 620
Val Lys Ala Glu Lys Gly Phe Val Gly Phe Pro His Val Ile Arg Gln
625 630 635 640
Asn Gln Asp Thr Asp His Pro Glu Asn Ile Glu Thr Tyr Glu Thr Val
645 650 655
Ser Ala Phe Ile Thr Thr Asp Leu Lys Lys Tyr Cys Leu Asn Trp Arg
660 665 670
Tyr Glu Thr Ile Ser Leu Phe Ala Gln Arg Leu Asn Glu Ile Tyr Gly
675 680 685
Leu Pro Ser Phe Phe Gln Trp Leu His Lys Arg Leu Glu Thr Ser Val
690 695 700
Leu Tyr Val Ser Asp Pro His Cys Pro Pro Asp Leu Asp Ala His Val
705 710 715 720
Pro Leu Cys Lys Val Pro Asn Asp Gln Ile Phe Ile Lys Tyr Pro Met
725 730 735
Gly Gly Ile Glu Gly Tyr Cys Gln Lys Leu Trp Thr Ile Ser Thr Ile
740 745 750
Pro Tyr Leu Tyr Leu Ala Ala Tyr Glu Ser Arg Val Arg Ile Ala Ser
755 760 765
Leu Val Gln Gly Asp Asn Gln Thr Ile Ala Val Thr Lys Arg Val Pro
770 775 780
Ser Thr Trp His Tyr Asn Leu Lys Lys Arg Glu Ala Ala Arg Val Thr
785 790 795 800
Arg Asp Tyr Phe Val Ile Leu Arg Gln Arg Leu His Asp Ile Gly His
805 810 815
His Leu Lys Ala Asn Glu Thr Ile Val Ser Ser His Phe Phe Val Tyr
820 825 830
Ser Lys Gly Ile Tyr Tyr Asp Gly Leu Leu Val Ser Gln Ser Leu Lys
835 840 845
Ser Ile Ala Arg Cys Val Phe Trp Ser Glu Thr Ile Val Asp Glu Thr
850 855 860
Arg Ala Ala Cys Ser Asn Ile Ala Thr Thr Met Ala Lys Ser Ile Glu
865 870 875 880
Arg Gly Tyr Asp Arg Tyr Leu Ala Tyr Ser Leu Asn Val Leu Lys Val
885 890 895
Ile Gln Gln Ile Leu Ile Ser Leu Gly Phe Thr Ile Asn Ser Thr Met
900 905 910
Thr Arg Asp Val Val Ile Pro Leu Leu Thr Asn Asn Asp Leu Leu Ile
915 920 925
Arg Met Ala Leu Leu Pro Ala Pro Ile Gly Gly Met Asn Tyr Leu Asn
930 935 940
Met Ser Arg Leu Phe Val Arg Asn Ile Gly Asp Pro Val Thr Ser Ser
945 950 955 960
Ile Ala Asp Leu Lys Arg Met Ile Leu Ala Ser Leu Met Pro Glu Glu
965 970 975
Thr Leu His Gln Val Met Thr Gln Gln Pro Gly Asp Ser Ser Phe Leu
980 985 990
Asp Trp Ala Ser Asp Pro Tyr Ser Ala Asn Leu Val Cys Val Gln Ser
995 1000 1005
Ile Thr Arg Leu Leu Lys Asn Ile Thr Ala Arg Phe Val Leu Ile
1010 1015 1020
His Ser Pro Asn Pro Met Leu Lys Gly Leu Phe His Asp Asp Ser
1025 1030 1035
Lys Glu Glu Asp Glu Arg Leu Ala Ala Phe Leu Met Asp Arg His
1040 1045 1050
Ile Ile Val Pro Arg Ala Ala His Glu Ile Leu Asp His Ser Val
1055 1060 1065
Thr Gly Ala Arg Glu Ser Ile Ala Gly Met Leu Asp Thr Thr Lys
1070 1075 1080
Gly Leu Ile Arg Ala Ser Met Arg Lys Gly Gly Leu Thr Ser Arg
1085 1090 1095
Val Ile Thr Arg Leu Ser Asn Tyr Asp Tyr Glu Gln Phe Arg Ala
1100 1105 1110
Gly Met Val Leu Leu Thr Gly Arg Lys Arg Asn Val Leu Ile Asp
1115 1120 1125
Lys Glu Ser Cys Ser Val Gln Leu Ala Arg Ala Leu Arg Ser His
1130 1135 1140
Met Trp Ala Arg Leu Ala Arg Gly Arg Pro Ile Tyr Gly Leu Glu
1145 1150 1155
Val Pro Asp Val Leu Glu Ser Met Arg Gly His Leu Ile Arg Arg
1160 1165 1170
His Glu Thr Cys Val Ile Cys Glu Cys Gly Ser Val Asn Tyr Gly
1175 1180 1185
Trp Phe Phe Val Pro Ser Gly Cys Gln Leu Asp Asp Ile Asp Lys
1190 1195 1200
Glu Thr Ser Ser Leu Arg Val Pro Tyr Ile Gly Ser Thr Thr Asp
1205 1210 1215
Glu Arg Thr Asp Met Lys Leu Ala Phe Val Arg Ala Pro Ser Arg
1220 1225 1230
Ser Leu Arg Ser Ala Val Arg Ile Ala Thr Val Tyr Ser Trp Ala
1235 1240 1245
Tyr Gly Asp Asp Asp Ser Ser Trp Asn Glu Ala Trp Leu Leu Ala
1250 1255 1260
Arg Gln Arg Ala Asn Val Ser Leu Glu Glu Leu Arg Val Ile Thr
1265 1270 1275
Pro Ile Ser Thr Ser Thr Asn Leu Ala His Arg Leu Arg Asp Arg
1280 1285 1290
Ser Thr Gln Val Lys Tyr Ser Gly Thr Ser Leu Val Arg Val Ala
1295 1300 1305
Arg Tyr Thr Thr Ile Ser Asn Asp Asn Leu Ser Phe Val Ile Ser
1310 1315 1320
Asp Lys Lys Val Asp Thr Asn Phe Ile Tyr Gln Gln Gly Met Leu
1325 1330 1335
Leu Gly Leu Gly Val Leu Glu Thr Leu Phe Arg Leu Glu Lys Asp
1340 1345 1350
Thr Gly Ser Ser Asn Thr Val Leu His Leu His Val Glu Thr Asp
1355 1360 1365
Cys Cys Val Ile Pro Met Ile Asp His Pro Arg Ile Pro Ser Ser
1370 1375 1380
Arg Lys Leu Glu Leu Arg Ala Glu Leu Cys Thr Asn Pro Leu Ile
1385 1390 1395
Tyr Asp Asn Ala Pro Leu Ile Asp Arg Asp Ala Thr Arg Leu Tyr
1400 1405 1410
Thr Gln Ser His Arg Arg His Leu Val Glu Phe Val Thr Trp Ser
1415 1420 1425
Thr Pro Gln Leu Tyr His Ile Leu Ala Lys Ser Thr Ala Leu Ser
1430 1435 1440
Met Ile Asp Leu Val Thr Lys Phe Glu Lys Asp His Met Asn Glu
1445 1450 1455
Ile Ser Ala Leu Ile Gly Asp Asp Asp Ile Asn Ser Phe Ile Thr
1460 1465 1470
Glu Phe Leu Leu Ile Glu Pro Arg Leu Phe Thr Ile Tyr Leu Gly
1475 1480 1485
Gln Cys Ala Ala Ile Asn Trp Ala Phe Asp Val His Tyr His Arg
1490 1495 1500
Pro Ser Gly Lys Tyr Gln Met Gly Glu Leu Leu Ser Ser Phe Leu
1505 1510 1515
Ser Arg Met Ser Lys Gly Val Phe Lys Val Leu Val Asn Ala Leu
1520 1525 1530
Ser His Pro Lys Ile Tyr Lys Lys Phe Trp His Cys Gly Ile Ile
1535 1540 1545
Glu Pro Ile His Gly Pro Ser Leu Asp Ala Gln Asn Leu His Thr
1550 1555 1560
Thr Val Cys Asn Met Val Tyr Thr Cys Tyr Met Thr Tyr Leu Asp
1565 1570 1575
Leu Leu Leu Asn Glu Glu Leu Glu Glu Phe Thr Phe Leu Leu Cys
1580 1585 1590
Glu Ser Asp Glu Asp Val Val Pro Asp Arg Phe Asp Asn Ile Gln
1595 1600 1605
Ala Lys His Leu Cys Val Leu Ala Asp Leu Tyr Cys Gln Pro Gly
1610 1615 1620
Thr Cys Pro Pro Ile Arg Gly Leu Arg Pro Val Glu Lys Cys Ala
1625 1630 1635
Val Leu Thr Asp His Ile Lys Ala Glu Ala Arg Leu Ser Pro Ala
1640 1645 1650
Gly Ser Ser Trp Asn Ile Asn Pro Ile Ile Val Asp His Tyr Ser
1655 1660 1665
Cys Ser Leu Thr Tyr Leu Arg Arg Gly Ser Ile Lys Gln Ile Arg
1670 1675 1680
Leu Arg Val Asp Pro Gly Phe Ile Phe Asp Ala Leu Ala Glu Val
1685 1690 1695
Asn Val Ser Gln Pro Lys Val Gly Ser Asp Val Met Val Ser Val
1700 1705 1710
Ile Lys Pro Asp Met Lys Ile Lys Leu Arg Met Glu Gly Ala Val
1715 1720 1725
Asn Gly His Pro Phe Ala Ile Glu Gly Val Gly Leu Gly Lys Pro
1730 1735 1740
Phe Glu Gly Lys Gln Ser Met Asp Leu Lys Val Lys Glu Gly Gly
1745 1750 1755
Pro Leu Pro Phe Ala Tyr Asp Ile Leu Thr Thr Val Phe Cys Tyr
1760 1765 1770
Gly Asn Arg Val Phe Ala Lys Tyr Pro Glu Asn Ile Val Asp Tyr
1775 1780 1785
Phe Lys Gln Ser Phe Pro Glu Gly Tyr Ser Trp Glu Arg Ser Met
1790 1795 1800
Asn Tyr Glu Asp Gly Gly Ile Cys Asn Ala Thr Asn Asp Ile Thr
1805 1810 1815
Leu Asp Gly Asp Cys Tyr Ile Tyr Glu Ile Arg Phe Asp Gly Val
1820 1825 1830
Asn Phe Pro Ala Asn Gly Pro Val Met Gln Lys Arg Thr Val Lys
1835 1840 1845
Trp Glu Pro Ser Thr Glu Lys Leu Tyr Val Arg Asp Gly Val Leu
1850 1855 1860
Lys Gly Asp Val Asn Met Ala Leu Ser Leu Glu Gly Gly Gly His
1865 1870 1875
Tyr Arg Cys Asp Phe Lys Thr Thr Tyr Lys Ala Lys Lys Val Val
1880 1885 1890
Gln Leu Pro Asp Tyr His Phe Val Asp His His Ile Glu Ile Lys
1895 1900 1905
Ser His Asp Lys Asp Tyr Ser Asn Val Asn Leu His Glu His Ala
1910 1915 1920
Glu Ala His Ser Glu Leu Pro Arg Gln Ala Lys Thr Gly Asn Ser
1925 1930 1935
Ala Asp Gly Gly Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly
1940 1945 1950
Gly Ser Thr Gln Gly Gly Ser Met Val Ser Val Ile Lys Pro Asp
1955 1960 1965
Met Lys Ile Lys Leu Arg Met Glu Gly Ala Val Asn Gly His Pro
1970 1975 1980
Phe Ala Ile Glu Gly Val Gly Leu Gly Lys Pro Phe Glu Gly Lys
1985 1990 1995
Gln Ser Met Asp Leu Lys Val Lys Glu Gly Gly Pro Leu Pro Phe
2000 2005 2010
Ala Tyr Asp Ile Leu Thr Thr Val Phe Cys Tyr Gly Asn Arg Val
2015 2020 2025
Phe Ala Lys Tyr Pro Glu Asn Ile Val Asp Tyr Phe Lys Gln Ser
2030 2035 2040
Phe Pro Glu Gly Tyr Ser Trp Glu Arg Ser Met Asn Tyr Glu Asp
2045 2050 2055
Gly Gly Ile Cys Asn Ala Thr Asn Asp Ile Thr Leu Asp Gly Asp
2060 2065 2070
Cys Tyr Ile Tyr Glu Ile Arg Phe Asp Gly Val Asn Phe Pro Ala
2075 2080 2085
Asn Gly Pro Val Met Gln Lys Arg Thr Val Lys Trp Glu Pro Ser
2090 2095 2100
Thr Glu Asn Leu Tyr Val Arg Asp Gly Val Leu Lys Gly Asp Val
2105 2110 2115
Asn Met Ala Leu Ser Leu Glu Gly Gly Gly His Tyr Arg Cys Asp
2120 2125 2130
Phe Lys Thr Thr Tyr Lys Ala Lys Lys Val Val Gln Leu Pro Asp
2135 2140 2145
Tyr His Phe Val Asp His His Ile Glu Ile Lys Ser His Asp Lys
2150 2155 2160
Asp Tyr Ser Asn Val Asn Leu His Glu His Ala Glu Ala His Ser
2165 2170 2175
Glu Leu Pro Arg Gln Ala Lys Asp Val Ile Ser Asn Met Ser Ile
2180 2185 2190
Lys Asp Phe Arg Pro Pro His Asp Asp Val Ala Lys Leu Leu Lys
2195 2200 2205
Asp Ile Asn Thr Ser Lys His Asn Leu Pro Ile Ser Gly Gly Ser
2210 2215 2220
Leu Ala Asn Tyr Glu Ile His Ala Phe Arg Arg Ile Gly Leu Asn
2225 2230 2235
Ser Ser Ala Cys Tyr Lys Ala Val Glu Ile Ser Thr Leu Ile Arg
2240 2245 2250
Arg Cys Leu Glu Pro Gly Glu Asp Gly Leu Phe Leu Gly Glu Gly
2255 2260 2265
Ser Gly Ser Met Leu Ile Thr Tyr Lys Glu Ile Leu Lys Leu Asn
2270 2275 2280
Lys Cys Phe Tyr Asn Ser Gly Val Ser Ala Asn Ser Arg Ser Gly
2285 2290 2295
Gln Arg Glu Leu Ala Pro Tyr Pro Ser Glu Val Gly Leu Val Glu
2300 2305 2310
His Arg Met Gly Val Gly Asn Ile Val Lys Val Leu Phe Asn Gly
2315 2320 2325
Arg Pro Glu Val Thr Trp Val Gly Ser Ile Asp Cys Phe Asn Phe
2330 2335 2340
Ile Val Ser Asn Ile Pro Thr Ser Ser Val Gly Phe Ile His Ser
2345 2350 2355
Asp Ile Glu Thr Leu Pro Asn Lys Asp Thr Ile Glu Lys Leu Glu
2360 2365 2370
Glu Leu Ala Ala Ile Leu Ser Met Ala Leu Leu Leu Gly Lys Ile
2375 2380 2385
Gly Ser Ile Leu Val Ile Lys Leu Met Pro Phe Ser Gly Asp Phe
2390 2395 2400
Val Gln Gly Phe Ile Ser Tyr Val Gly Ser His Tyr Arg Glu Val
2405 2410 2415
Asn Leu Val Tyr Pro Arg Tyr Ser Asn Phe Ile Ser Thr Glu Ser
2420 2425 2430
Tyr Leu Val Met Thr Asp Leu Lys Ala Asn Arg Leu Met Asn Pro
2435 2440 2445
Glu Lys Ile Lys Gln Gln Ile Ile Glu Ser Ser Val Arg Thr Ser
2450 2455 2460
Pro Gly Leu Ile Gly His Ile Leu Ser Ile Lys Gln Leu Ser Cys
2465 2470 2475
Ile Gln Ala Ile Val Gly Gly Ala Val Ser Arg Gly Asp Ile Asn
2480 2485 2490
Pro Ile Leu Lys Lys Leu Thr Pro Ile Glu Gln Val Leu Ile Ser
2495 2500 2505
Cys Gly Leu Ala Ile Asn Gly Pro Lys Leu Cys Lys Glu Leu Ile
2510 2515 2520
His His Asp Val Ala Ser Gly Gln Asp Gly Leu Leu Asn Ser Ile
2525 2530 2535
Leu Ile Leu Tyr Arg Glu Leu Ala Arg Phe Lys Asp Asn Gln Arg
2540 2545 2550
Ser Gln Gln Gly Met Phe His Ala Tyr Pro Val Leu Val Ser Ser
2555 2560 2565
Arg Gln Arg Glu Leu Val Ser Arg Ile Thr Arg Lys Phe Trp Gly
2570 2575 2580
His Ile Leu Leu Tyr Ser Gly Asn Arg Lys Leu Ile Asn Arg Phe
2585 2590 2595
Ile Gln Asn Leu Lys Ser Gly Tyr Leu Val Leu Asp Leu His Gln
2600 2605 2610
Asn Ile Phe Val Lys Asn Leu Ser Lys Ser Glu Lys Gln Ile Ile
2615 2620 2625
Met Thr Gly Gly Leu Lys Arg Glu Trp Val Phe Lys Val Thr Val
2630 2635 2640
Lys Glu Thr Lys Glu Trp Tyr Lys Leu Val Gly Tyr Ser Ala Leu
2645 2650 2655
Ile Lys Asp
2660
<210> 39
<211> 2521
<212> PRT
<213> Artificial Sequence
<220>
<223> Ln-pMag-nMaghigh-Lc
<400> 39
Met Asp Ser Leu Ser Val Asn Gln Ile Leu Tyr Pro Glu Val His Leu
1 5 10 15
Asp Ser Pro Ile Val Thr Asn Lys Ile Val Ala Ile Leu Glu Tyr Ala
20 25 30
Arg Val Pro His Ala Tyr Ser Leu Glu Asp Pro Thr Leu Cys Gln Asn
35 40 45
Ile Lys His Arg Leu Lys Asn Gly Phe Ser Asn Gln Met Ile Ile Asn
50 55 60
Asn Val Glu Val Gly Asn Val Ile Lys Ser Lys Leu Arg Ser Tyr Pro
65 70 75 80
Ala His Ser His Ile Pro Tyr Pro Asn Cys Asn Gln Asp Leu Phe Asn
85 90 95
Ile Glu Asp Lys Glu Ser Thr Arg Lys Ile Arg Glu Leu Leu Lys Lys
100 105 110
Gly Asn Ser Leu Tyr Ser Lys Val Ser Asp Lys Val Phe Gln Cys Leu
115 120 125
Arg Asp Thr Asn Ser Arg Leu Gly Leu Gly Ser Glu Leu Arg Glu Asp
130 135 140
Ile Lys Glu Lys Ile Ile Asn Leu Gly Val Tyr Met His Ser Ser Gln
145 150 155 160
Trp Phe Glu Pro Phe Leu Phe Trp Phe Thr Val Lys Thr Glu Met Arg
165 170 175
Ser Val Ile Lys Ser Gln Thr His Thr Cys His Arg Arg Arg His Thr
180 185 190
Pro Val Phe Phe Thr Gly Ser Ser Val Glu Leu Leu Ile Ser Arg Asp
195 200 205
Leu Val Ala Ile Ile Ser Lys Glu Ser Gln His Val Tyr Tyr Leu Thr
210 215 220
Phe Glu Leu Val Leu Met Tyr Cys Asp Val Ile Glu Gly Arg Leu Met
225 230 235 240
Thr Glu Thr Ala Met Thr Ile Asp Ala Arg Tyr Ala Glu Leu Leu Gly
245 250 255
Arg Val Arg Tyr Met Trp Lys Leu Ile Asp Gly Phe Phe Pro Ala Leu
260 265 270
Gly Asn Pro Thr Tyr Gln Ile Val Ala Met Leu Glu Pro Leu Ser Leu
275 280 285
Ala Tyr Leu Gln Leu Arg Asp Ile Thr Val Glu Leu Arg Gly Ala Phe
290 295 300
Leu Asn His Cys Phe Thr Glu Ile His Asp Val Leu Asp Gln Asn Gly
305 310 315 320
Phe Ser Asp Glu Gly Thr Tyr His Glu Leu Ile Glu Ala Leu Asp Tyr
325 330 335
Ile Phe Ile Thr Asp Asp Ile His Leu Thr Gly Glu Ile Phe Ser Phe
340 345 350
Phe Arg Ser Phe Gly His Pro Arg Leu Glu Ala Val Thr Ala Ala Glu
355 360 365
Asn Val Arg Lys Tyr Met Asn Gln Pro Lys Val Ile Val Tyr Glu Thr
370 375 380
Leu Met Lys Gly His Ala Ile Phe Cys Gly Ile Ile Ile Asn Gly Tyr
385 390 395 400
Arg Asp Arg His Gly Gly Ser Trp Pro Pro Leu Thr Leu Pro Leu His
405 410 415
Ala Ala Asp Thr Ile Arg Asn Ala Gln Ala Ser Gly Glu Gly Leu Thr
420 425 430
His Glu Gln Cys Val Asp Asn Trp Lys Ser Phe Ala Gly Val Arg Phe
435 440 445
Gly Cys Phe Met Pro Leu Ser Leu Asp Ser Asp Leu Thr Met Tyr Leu
450 455 460
Lys Asp Lys Ala Leu Ala Ala Leu Gln Arg Glu Trp Asp Ser Val Tyr
465 470 475 480
Pro Lys Glu Phe Leu Arg Tyr Asp Pro Pro Lys Gly Thr Gly Ser Arg
485 490 495
Arg Leu Val Asp Val Phe Leu Asn Asp Ser Ser Phe Asp Pro Tyr Asp
500 505 510
Met Ile Met Tyr Val Val Ser Gly Ala Tyr Leu His Asp Pro Glu Phe
515 520 525
Asn Leu Ser Tyr Ser Leu Lys Glu Lys Glu Ile Lys Glu Thr Gly Arg
530 535 540
Leu Phe Ala Lys Met Thr Tyr Lys Met Arg Ala Cys Gln Val Ile Ala
545 550 555 560
Glu Asn Leu Ile Ser Asn Gly Ile Gly Lys Tyr Phe Lys Asp Asn Gly
565 570 575
Met Ala Lys Asp Glu His Asp Leu Thr Lys Ala Leu His Thr Leu Ala
580 585 590
Val Ser Gly Val Pro Lys Asp Leu Lys Glu Ser His Arg Gly Gly Pro
595 600 605
Val Leu Lys Thr Tyr Ser Arg Ser Pro Val His Thr Ser Thr Arg Asn
610 615 620
Val Lys Ala Glu Lys Gly Phe Val Gly Phe Pro His Val Ile Arg Gln
625 630 635 640
Asn Gln Asp Thr Asp His Pro Glu Asn Ile Glu Thr Tyr Glu Thr Val
645 650 655
Ser Ala Phe Ile Thr Thr Asp Leu Lys Lys Tyr Cys Leu Asn Trp Arg
660 665 670
Tyr Glu Thr Ile Ser Leu Phe Ala Gln Arg Leu Asn Glu Ile Tyr Gly
675 680 685
Leu Pro Ser Phe Phe Gln Trp Leu His Lys Arg Leu Glu Thr Ser Val
690 695 700
Leu Tyr Val Ser Asp Pro His Cys Pro Pro Asp Leu Asp Ala His Val
705 710 715 720
Pro Leu Cys Lys Val Pro Asn Asp Gln Ile Phe Ile Lys Tyr Pro Met
725 730 735
Gly Gly Ile Glu Gly Tyr Cys Gln Lys Leu Trp Thr Ile Ser Thr Ile
740 745 750
Pro Tyr Leu Tyr Leu Ala Ala Tyr Glu Ser Arg Val Arg Ile Ala Ser
755 760 765
Leu Val Gln Gly Asp Asn Gln Thr Ile Ala Val Thr Lys Arg Val Pro
770 775 780
Ser Thr Trp His Tyr Asn Leu Lys Lys Arg Glu Ala Ala Arg Val Thr
785 790 795 800
Arg Asp Tyr Phe Val Ile Leu Arg Gln Arg Leu His Asp Ile Gly His
805 810 815
His Leu Lys Ala Asn Glu Thr Ile Val Ser Ser His Phe Phe Val Tyr
820 825 830
Ser Lys Gly Ile Tyr Tyr Asp Gly Leu Leu Val Ser Gln Ser Leu Lys
835 840 845
Ser Ile Ala Arg Cys Val Phe Trp Ser Glu Thr Ile Val Asp Glu Thr
850 855 860
Arg Ala Ala Cys Ser Asn Ile Ala Thr Thr Met Ala Lys Ser Ile Glu
865 870 875 880
Arg Gly Tyr Asp Arg Tyr Leu Ala Tyr Ser Leu Asn Val Leu Lys Val
885 890 895
Ile Gln Gln Ile Leu Ile Ser Leu Gly Phe Thr Ile Asn Ser Thr Met
900 905 910
Thr Arg Asp Val Val Ile Pro Leu Leu Thr Asn Asn Asp Leu Leu Ile
915 920 925
Arg Met Ala Leu Leu Pro Ala Pro Ile Gly Gly Met Asn Tyr Leu Asn
930 935 940
Met Ser Arg Leu Phe Val Arg Asn Ile Gly Asp Pro Val Thr Ser Ser
945 950 955 960
Ile Ala Asp Leu Lys Arg Met Ile Leu Ala Ser Leu Met Pro Glu Glu
965 970 975
Thr Leu His Gln Val Met Thr Gln Gln Pro Gly Asp Ser Ser Phe Leu
980 985 990
Asp Trp Ala Ser Asp Pro Tyr Ser Ala Asn Leu Val Cys Val Gln Ser
995 1000 1005
Ile Thr Arg Leu Leu Lys Asn Ile Thr Ala Arg Phe Val Leu Ile
1010 1015 1020
His Ser Pro Asn Pro Met Leu Lys Gly Leu Phe His Asp Asp Ser
1025 1030 1035
Lys Glu Glu Asp Glu Arg Leu Ala Ala Phe Leu Met Asp Arg His
1040 1045 1050
Ile Ile Val Pro Arg Ala Ala His Glu Ile Leu Asp His Ser Val
1055 1060 1065
Thr Gly Ala Arg Glu Ser Ile Ala Gly Met Leu Asp Thr Thr Lys
1070 1075 1080
Gly Leu Ile Arg Ala Ser Met Arg Lys Gly Gly Leu Thr Ser Arg
1085 1090 1095
Val Ile Thr Arg Leu Ser Asn Tyr Asp Tyr Glu Gln Phe Arg Ala
1100 1105 1110
Gly Met Val Leu Leu Thr Gly Arg Lys Arg Asn Val Leu Ile Asp
1115 1120 1125
Lys Glu Ser Cys Ser Val Gln Leu Ala Arg Ala Leu Arg Ser His
1130 1135 1140
Met Trp Ala Arg Leu Ala Arg Gly Arg Pro Ile Tyr Gly Leu Glu
1145 1150 1155
Val Pro Asp Val Leu Glu Ser Met Arg Gly His Leu Ile Arg Arg
1160 1165 1170
His Glu Thr Cys Val Ile Cys Glu Cys Gly Ser Val Asn Tyr Gly
1175 1180 1185
Trp Phe Phe Val Pro Ser Gly Cys Gln Leu Asp Asp Ile Asp Lys
1190 1195 1200
Glu Thr Ser Ser Leu Arg Val Pro Tyr Ile Gly Ser Thr Thr Asp
1205 1210 1215
Glu Arg Thr Asp Met Lys Leu Ala Phe Val Arg Ala Pro Ser Arg
1220 1225 1230
Ser Leu Arg Ser Ala Val Arg Ile Ala Thr Val Tyr Ser Trp Ala
1235 1240 1245
Tyr Gly Asp Asp Asp Ser Ser Trp Asn Glu Ala Trp Leu Leu Ala
1250 1255 1260
Arg Gln Arg Ala Asn Val Ser Leu Glu Glu Leu Arg Val Ile Thr
1265 1270 1275
Pro Ile Ser Thr Ser Thr Asn Leu Ala His Arg Leu Arg Asp Arg
1280 1285 1290
Ser Thr Gln Val Lys Tyr Ser Gly Thr Ser Leu Val Arg Val Ala
1295 1300 1305
Arg Tyr Thr Thr Ile Ser Asn Asp Asn Leu Ser Phe Val Ile Ser
1310 1315 1320
Asp Lys Lys Val Asp Thr Asn Phe Ile Tyr Gln Gln Gly Met Leu
1325 1330 1335
Leu Gly Leu Gly Val Leu Glu Thr Leu Phe Arg Leu Glu Lys Asp
1340 1345 1350
Thr Gly Ser Ser Asn Thr Val Leu His Leu His Val Glu Thr Asp
1355 1360 1365
Cys Cys Val Ile Pro Met Ile Asp His Pro Arg Ile Pro Ser Ser
1370 1375 1380
Arg Lys Leu Glu Leu Arg Ala Glu Leu Cys Thr Asn Pro Leu Ile
1385 1390 1395
Tyr Asp Asn Ala Pro Leu Ile Asp Arg Asp Ala Thr Arg Leu Tyr
1400 1405 1410
Thr Gln Ser His Arg Arg His Leu Val Glu Phe Val Thr Trp Ser
1415 1420 1425
Thr Pro Gln Leu Tyr His Ile Leu Ala Lys Ser Thr Ala Leu Ser
1430 1435 1440
Met Ile Asp Leu Val Thr Lys Phe Glu Lys Asp His Met Asn Glu
1445 1450 1455
Ile Ser Ala Leu Ile Gly Asp Asp Asp Ile Asn Ser Phe Ile Thr
1460 1465 1470
Glu Phe Leu Leu Ile Glu Pro Arg Leu Phe Thr Ile Tyr Leu Gly
1475 1480 1485
Gln Cys Ala Ala Ile Asn Trp Ala Phe Asp Val His Tyr His Arg
1490 1495 1500
Pro Ser Gly Lys Tyr Gln Met Gly Glu Leu Leu Ser Ser Phe Leu
1505 1510 1515
Ser Arg Met Ser Lys Gly Val Phe Lys Val Leu Val Asn Ala Leu
1520 1525 1530
Ser His Pro Lys Ile Tyr Lys Lys Phe Trp His Cys Gly Ile Ile
1535 1540 1545
Glu Pro Ile His Gly Pro Ser Leu Asp Ala Gln Asn Leu His Thr
1550 1555 1560
Thr Val Cys Asn Met Val Tyr Thr Cys Tyr Met Thr Tyr Leu Asp
1565 1570 1575
Leu Leu Leu Asn Glu Glu Leu Glu Glu Phe Thr Phe Leu Leu Cys
1580 1585 1590
Glu Ser Asp Glu Asp Val Val Pro Asp Arg Phe Asp Asn Ile Gln
1595 1600 1605
Ala Lys His Leu Cys Val Leu Ala Asp Leu Tyr Cys Gln Pro Gly
1610 1615 1620
Thr Cys Pro Pro Ile Arg Gly Leu Arg Pro Val Glu Lys Cys Ala
1625 1630 1635
Val Leu Thr Asp His Ile Lys Ala Glu Ala Arg Leu Ser Pro Ala
1640 1645 1650
Gly Ser Ser Trp Asn Ile Asn Pro Ile Ile Val Asp His Tyr Ser
1655 1660 1665
Cys Ser Leu Thr Tyr Leu Arg Arg Gly Ser Ile Lys Gln Ile Arg
1670 1675 1680
Leu Arg Val Asp Pro Gly Phe Ile Phe Asp Ala Leu Ala Glu Val
1685 1690 1695
Asn Val Ser Gln Pro Lys Val Gly Ser Asp Val Ser His Thr Leu
1700 1705 1710
Tyr Ala Pro Gly Gly Tyr Asp Ile Met Gly Tyr Leu Arg Gln Ile
1715 1720 1725
Arg Asn Arg Pro Asn Pro Gln Val Glu Leu Gly Pro Val Asp Thr
1730 1735 1740
Ser Cys Ala Leu Ile Leu Cys Asp Leu Lys Gln Lys Asp Thr Pro
1745 1750 1755
Ile Val Tyr Ala Ser Glu Ala Phe Leu Tyr Met Thr Gly Tyr Ser
1760 1765 1770
Asn Ala Glu Val Leu Gly Arg Asn Cys Arg Phe Leu Gln Ser Pro
1775 1780 1785
Asp Gly Met Val Lys Pro Lys Ser Thr Arg Lys Tyr Val Asp Ser
1790 1795 1800
Asn Thr Ile Asn Thr Met Arg Lys Ala Ile Asp Arg Asn Ala Glu
1805 1810 1815
Val Gln Val Glu Val Val Asn Phe Lys Lys Asn Gly Gln Arg Phe
1820 1825 1830
Val Asn Phe Leu Thr Met Ile Pro Val Arg Asp Glu Thr Gly Glu
1835 1840 1845
Tyr Arg Tyr Ser Met Gly Phe Gln Cys Glu Thr Glu Thr Gly Asn
1850 1855 1860
Ser Ala Asp Gly Gly Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly
1865 1870 1875
Gly Gly Ser Thr Gln Gly Gly Ser Met His Thr Leu Tyr Ala Pro
1880 1885 1890
Gly Gly Tyr Asp Ile Met Gly Tyr Leu Asp Gln Ile Gly Asn Arg
1895 1900 1905
Pro Asn Pro Gln Val Glu Leu Gly Pro Val Asp Thr Ser Cys Ala
1910 1915 1920
Leu Ile Leu Cys Asp Leu Lys Gln Lys Asp Thr Pro Ile Val Tyr
1925 1930 1935
Ala Ser Glu Ala Phe Leu Tyr Met Thr Gly Tyr Ser Asn Ala Glu
1940 1945 1950
Val Leu Gly Arg Asn Cys Arg Phe Leu Gln Ser Pro Asp Gly Met
1955 1960 1965
Val Lys Pro Lys Ser Thr Arg Lys Tyr Val Asp Ser Asn Thr Ile
1970 1975 1980
Asn Thr Ile Arg Lys Ala Ile Asp Arg Asn Ala Glu Val Gln Val
1985 1990 1995
Glu Val Val Asn Phe Lys Lys Asn Gly Gln Arg Phe Val Asn Phe
2000 2005 2010
Leu Thr Ile Ile Pro Val Arg Asp Glu Thr Gly Glu Tyr Arg Tyr
2015 2020 2025
Ser Met Gly Phe Gln Cys Glu Thr Glu Tyr Pro Tyr Asp Val Pro
2030 2035 2040
Asp Tyr Ala Gly Ile Ser Asn Met Ser Ile Lys Asp Phe Arg Pro
2045 2050 2055
Pro His Asp Asp Val Ala Lys Leu Leu Lys Asp Ile Asn Thr Ser
2060 2065 2070
Lys His Asn Leu Pro Ile Ser Gly Gly Ser Leu Ala Asn Tyr Glu
2075 2080 2085
Ile His Ala Phe Arg Arg Ile Gly Leu Asn Ser Ser Ala Cys Tyr
2090 2095 2100
Lys Ala Val Glu Ile Ser Thr Leu Ile Arg Arg Cys Leu Glu Pro
2105 2110 2115
Gly Glu Asp Gly Leu Phe Leu Gly Glu Gly Ser Gly Ser Met Leu
2120 2125 2130
Ile Thr Tyr Lys Glu Ile Leu Lys Leu Asn Lys Cys Phe Tyr Asn
2135 2140 2145
Ser Gly Val Ser Ala Asn Ser Arg Ser Gly Gln Arg Glu Leu Ala
2150 2155 2160
Pro Tyr Pro Ser Glu Val Gly Leu Val Glu His Arg Met Gly Val
2165 2170 2175
Gly Asn Ile Val Lys Val Leu Phe Asn Gly Arg Pro Glu Val Thr
2180 2185 2190
Trp Val Gly Ser Ile Asp Cys Phe Asn Phe Ile Val Ser Asn Ile
2195 2200 2205
Pro Thr Ser Ser Val Gly Phe Ile His Ser Asp Ile Glu Thr Leu
2210 2215 2220
Pro Asn Lys Asp Thr Ile Glu Lys Leu Glu Glu Leu Ala Ala Ile
2225 2230 2235
Leu Ser Met Ala Leu Leu Leu Gly Lys Ile Gly Ser Ile Leu Val
2240 2245 2250
Ile Lys Leu Met Pro Phe Ser Gly Asp Phe Val Gln Gly Phe Ile
2255 2260 2265
Ser Tyr Val Gly Ser His Tyr Arg Glu Val Asn Leu Val Tyr Pro
2270 2275 2280
Arg Tyr Ser Asn Phe Ile Ser Thr Glu Ser Tyr Leu Val Met Thr
2285 2290 2295
Asp Leu Lys Ala Asn Arg Leu Met Asn Pro Glu Lys Ile Lys Gln
2300 2305 2310
Gln Ile Ile Glu Ser Ser Val Arg Thr Ser Pro Gly Leu Ile Gly
2315 2320 2325
His Ile Leu Ser Ile Lys Gln Leu Ser Cys Ile Gln Ala Ile Val
2330 2335 2340
Gly Gly Ala Val Ser Arg Gly Asp Ile Asn Pro Ile Leu Lys Lys
2345 2350 2355
Leu Thr Pro Ile Glu Gln Val Leu Ile Ser Cys Gly Leu Ala Ile
2360 2365 2370
Asn Gly Pro Lys Leu Cys Lys Glu Leu Ile His His Asp Val Ala
2375 2380 2385
Ser Gly Gln Asp Gly Leu Leu Asn Ser Ile Leu Ile Leu Tyr Arg
2390 2395 2400
Glu Leu Ala Arg Phe Lys Asp Asn Gln Arg Ser Gln Gln Gly Met
2405 2410 2415
Phe His Ala Tyr Pro Val Leu Val Ser Ser Arg Gln Arg Glu Leu
2420 2425 2430
Val Ser Arg Ile Thr Arg Lys Phe Trp Gly His Ile Leu Leu Tyr
2435 2440 2445
Ser Gly Asn Arg Lys Leu Ile Asn Arg Phe Ile Gln Asn Leu Lys
2450 2455 2460
Ser Gly Tyr Leu Val Leu Asp Leu His Gln Asn Ile Phe Val Lys
2465 2470 2475
Asn Leu Ser Lys Ser Glu Lys Gln Ile Ile Met Thr Gly Gly Leu
2480 2485 2490
Lys Arg Glu Trp Val Phe Lys Val Thr Val Lys Glu Thr Lys Glu
2495 2500 2505
Trp Tyr Lys Leu Val Gly Tyr Ser Ala Leu Ile Lys Asp
2510 2515 2520
<210> 40
<211> 3001
<212> PRT
<213> Artificial Sequence
<220>
<223> Ln-CRY2-CIB1N-Lc
<400> 40
Met Asp Ser Leu Ser Val Asn Gln Ile Leu Tyr Pro Glu Val His Leu
1 5 10 15
Asp Ser Pro Ile Val Thr Asn Lys Ile Val Ala Ile Leu Glu Tyr Ala
20 25 30
Arg Val Pro His Ala Tyr Ser Leu Glu Asp Pro Thr Leu Cys Gln Asn
35 40 45
Ile Lys His Arg Leu Lys Asn Gly Phe Ser Asn Gln Met Ile Ile Asn
50 55 60
Asn Val Glu Val Gly Asn Val Ile Lys Ser Lys Leu Arg Ser Tyr Pro
65 70 75 80
Ala His Ser His Ile Pro Tyr Pro Asn Cys Asn Gln Asp Leu Phe Asn
85 90 95
Ile Glu Asp Lys Glu Ser Thr Arg Lys Ile Arg Glu Leu Leu Lys Lys
100 105 110
Gly Asn Ser Leu Tyr Ser Lys Val Ser Asp Lys Val Phe Gln Cys Leu
115 120 125
Arg Asp Thr Asn Ser Arg Leu Gly Leu Gly Ser Glu Leu Arg Glu Asp
130 135 140
Ile Lys Glu Lys Ile Ile Asn Leu Gly Val Tyr Met His Ser Ser Gln
145 150 155 160
Trp Phe Glu Pro Phe Leu Phe Trp Phe Thr Val Lys Thr Glu Met Arg
165 170 175
Ser Val Ile Lys Ser Gln Thr His Thr Cys His Arg Arg Arg His Thr
180 185 190
Pro Val Phe Phe Thr Gly Ser Ser Val Glu Leu Leu Ile Ser Arg Asp
195 200 205
Leu Val Ala Ile Ile Ser Lys Glu Ser Gln His Val Tyr Tyr Leu Thr
210 215 220
Phe Glu Leu Val Leu Met Tyr Cys Asp Val Ile Glu Gly Arg Leu Met
225 230 235 240
Thr Glu Thr Ala Met Thr Ile Asp Ala Arg Tyr Ala Glu Leu Leu Gly
245 250 255
Arg Val Arg Tyr Met Trp Lys Leu Ile Asp Gly Phe Phe Pro Ala Leu
260 265 270
Gly Asn Pro Thr Tyr Gln Ile Val Ala Met Leu Glu Pro Leu Ser Leu
275 280 285
Ala Tyr Leu Gln Leu Arg Asp Ile Thr Val Glu Leu Arg Gly Ala Phe
290 295 300
Leu Asn His Cys Phe Thr Glu Ile His Asp Val Leu Asp Gln Asn Gly
305 310 315 320
Phe Ser Asp Glu Gly Thr Tyr His Glu Leu Ile Glu Ala Leu Asp Tyr
325 330 335
Ile Phe Ile Thr Asp Asp Ile His Leu Thr Gly Glu Ile Phe Ser Phe
340 345 350
Phe Arg Ser Phe Gly His Pro Arg Leu Glu Ala Val Thr Ala Ala Glu
355 360 365
Asn Val Arg Lys Tyr Met Asn Gln Pro Lys Val Ile Val Tyr Glu Thr
370 375 380
Leu Met Lys Gly His Ala Ile Phe Cys Gly Ile Ile Ile Asn Gly Tyr
385 390 395 400
Arg Asp Arg His Gly Gly Ser Trp Pro Pro Leu Thr Leu Pro Leu His
405 410 415
Ala Ala Asp Thr Ile Arg Asn Ala Gln Ala Ser Gly Glu Gly Leu Thr
420 425 430
His Glu Gln Cys Val Asp Asn Trp Lys Ser Phe Ala Gly Val Arg Phe
435 440 445
Gly Cys Phe Met Pro Leu Ser Leu Asp Ser Asp Leu Thr Met Tyr Leu
450 455 460
Lys Asp Lys Ala Leu Ala Ala Leu Gln Arg Glu Trp Asp Ser Val Tyr
465 470 475 480
Pro Lys Glu Phe Leu Arg Tyr Asp Pro Pro Lys Gly Thr Gly Ser Arg
485 490 495
Arg Leu Val Asp Val Phe Leu Asn Asp Ser Ser Phe Asp Pro Tyr Asp
500 505 510
Met Ile Met Tyr Val Val Ser Gly Ala Tyr Leu His Asp Pro Glu Phe
515 520 525
Asn Leu Ser Tyr Ser Leu Lys Glu Lys Glu Ile Lys Glu Thr Gly Arg
530 535 540
Leu Phe Ala Lys Met Thr Tyr Lys Met Arg Ala Cys Gln Val Ile Ala
545 550 555 560
Glu Asn Leu Ile Ser Asn Gly Ile Gly Lys Tyr Phe Lys Asp Asn Gly
565 570 575
Met Ala Lys Asp Glu His Asp Leu Thr Lys Ala Leu His Thr Leu Ala
580 585 590
Val Ser Gly Val Pro Lys Asp Leu Lys Glu Ser His Arg Gly Gly Pro
595 600 605
Val Leu Lys Thr Tyr Ser Arg Ser Pro Val His Thr Ser Thr Arg Asn
610 615 620
Val Lys Ala Glu Lys Gly Phe Val Gly Phe Pro His Val Ile Arg Gln
625 630 635 640
Asn Gln Asp Thr Asp His Pro Glu Asn Ile Glu Thr Tyr Glu Thr Val
645 650 655
Ser Ala Phe Ile Thr Thr Asp Leu Lys Lys Tyr Cys Leu Asn Trp Arg
660 665 670
Tyr Glu Thr Ile Ser Leu Phe Ala Gln Arg Leu Asn Glu Ile Tyr Gly
675 680 685
Leu Pro Ser Phe Phe Gln Trp Leu His Lys Arg Leu Glu Thr Ser Val
690 695 700
Leu Tyr Val Ser Asp Pro His Cys Pro Pro Asp Leu Asp Ala His Val
705 710 715 720
Pro Leu Cys Lys Val Pro Asn Asp Gln Ile Phe Ile Lys Tyr Pro Met
725 730 735
Gly Gly Ile Glu Gly Tyr Cys Gln Lys Leu Trp Thr Ile Ser Thr Ile
740 745 750
Pro Tyr Leu Tyr Leu Ala Ala Tyr Glu Ser Arg Val Arg Ile Ala Ser
755 760 765
Leu Val Gln Gly Asp Asn Gln Thr Ile Ala Val Thr Lys Arg Val Pro
770 775 780
Ser Thr Trp His Tyr Asn Leu Lys Lys Arg Glu Ala Ala Arg Val Thr
785 790 795 800
Arg Asp Tyr Phe Val Ile Leu Arg Gln Arg Leu His Asp Ile Gly His
805 810 815
His Leu Lys Ala Asn Glu Thr Ile Val Ser Ser His Phe Phe Val Tyr
820 825 830
Ser Lys Gly Ile Tyr Tyr Asp Gly Leu Leu Val Ser Gln Ser Leu Lys
835 840 845
Ser Ile Ala Arg Cys Val Phe Trp Ser Glu Thr Ile Val Asp Glu Thr
850 855 860
Arg Ala Ala Cys Ser Asn Ile Ala Thr Thr Met Ala Lys Ser Ile Glu
865 870 875 880
Arg Gly Tyr Asp Arg Tyr Leu Ala Tyr Ser Leu Asn Val Leu Lys Val
885 890 895
Ile Gln Gln Ile Leu Ile Ser Leu Gly Phe Thr Ile Asn Ser Thr Met
900 905 910
Thr Arg Asp Val Val Ile Pro Leu Leu Thr Asn Asn Asp Leu Leu Ile
915 920 925
Arg Met Ala Leu Leu Pro Ala Pro Ile Gly Gly Met Asn Tyr Leu Asn
930 935 940
Met Ser Arg Leu Phe Val Arg Asn Ile Gly Asp Pro Val Thr Ser Ser
945 950 955 960
Ile Ala Asp Leu Lys Arg Met Ile Leu Ala Ser Leu Met Pro Glu Glu
965 970 975
Thr Leu His Gln Val Met Thr Gln Gln Pro Gly Asp Ser Ser Phe Leu
980 985 990
Asp Trp Ala Ser Asp Pro Tyr Ser Ala Asn Leu Val Cys Val Gln Ser
995 1000 1005
Ile Thr Arg Leu Leu Lys Asn Ile Thr Ala Arg Phe Val Leu Ile
1010 1015 1020
His Ser Pro Asn Pro Met Leu Lys Gly Leu Phe His Asp Asp Ser
1025 1030 1035
Lys Glu Glu Asp Glu Arg Leu Ala Ala Phe Leu Met Asp Arg His
1040 1045 1050
Ile Ile Val Pro Arg Ala Ala His Glu Ile Leu Asp His Ser Val
1055 1060 1065
Thr Gly Ala Arg Glu Ser Ile Ala Gly Met Leu Asp Thr Thr Lys
1070 1075 1080
Gly Leu Ile Arg Ala Ser Met Arg Lys Gly Gly Leu Thr Ser Arg
1085 1090 1095
Val Ile Thr Arg Leu Ser Asn Tyr Asp Tyr Glu Gln Phe Arg Ala
1100 1105 1110
Gly Met Val Leu Leu Thr Gly Arg Lys Arg Asn Val Leu Ile Asp
1115 1120 1125
Lys Glu Ser Cys Ser Val Gln Leu Ala Arg Ala Leu Arg Ser His
1130 1135 1140
Met Trp Ala Arg Leu Ala Arg Gly Arg Pro Ile Tyr Gly Leu Glu
1145 1150 1155
Val Pro Asp Val Leu Glu Ser Met Arg Gly His Leu Ile Arg Arg
1160 1165 1170
His Glu Thr Cys Val Ile Cys Glu Cys Gly Ser Val Asn Tyr Gly
1175 1180 1185
Trp Phe Phe Val Pro Ser Gly Cys Gln Leu Asp Asp Ile Asp Lys
1190 1195 1200
Glu Thr Ser Ser Leu Arg Val Pro Tyr Ile Gly Ser Thr Thr Asp
1205 1210 1215
Glu Arg Thr Asp Met Lys Leu Ala Phe Val Arg Ala Pro Ser Arg
1220 1225 1230
Ser Leu Arg Ser Ala Val Arg Ile Ala Thr Val Tyr Ser Trp Ala
1235 1240 1245
Tyr Gly Asp Asp Asp Ser Ser Trp Asn Glu Ala Trp Leu Leu Ala
1250 1255 1260
Arg Gln Arg Ala Asn Val Ser Leu Glu Glu Leu Arg Val Ile Thr
1265 1270 1275
Pro Ile Ser Thr Ser Thr Asn Leu Ala His Arg Leu Arg Asp Arg
1280 1285 1290
Ser Thr Gln Val Lys Tyr Ser Gly Thr Ser Leu Val Arg Val Ala
1295 1300 1305
Arg Tyr Thr Thr Ile Ser Asn Asp Asn Leu Ser Phe Val Ile Ser
1310 1315 1320
Asp Lys Lys Val Asp Thr Asn Phe Ile Tyr Gln Gln Gly Met Leu
1325 1330 1335
Leu Gly Leu Gly Val Leu Glu Thr Leu Phe Arg Leu Glu Lys Asp
1340 1345 1350
Thr Gly Ser Ser Asn Thr Val Leu His Leu His Val Glu Thr Asp
1355 1360 1365
Cys Cys Val Ile Pro Met Ile Asp His Pro Arg Ile Pro Ser Ser
1370 1375 1380
Arg Lys Leu Glu Leu Arg Ala Glu Leu Cys Thr Asn Pro Leu Ile
1385 1390 1395
Tyr Asp Asn Ala Pro Leu Ile Asp Arg Asp Ala Thr Arg Leu Tyr
1400 1405 1410
Thr Gln Ser His Arg Arg His Leu Val Glu Phe Val Thr Trp Ser
1415 1420 1425
Thr Pro Gln Leu Tyr His Ile Leu Ala Lys Ser Thr Ala Leu Ser
1430 1435 1440
Met Ile Asp Leu Val Thr Lys Phe Glu Lys Asp His Met Asn Glu
1445 1450 1455
Ile Ser Ala Leu Ile Gly Asp Asp Asp Ile Asn Ser Phe Ile Thr
1460 1465 1470
Glu Phe Leu Leu Ile Glu Pro Arg Leu Phe Thr Ile Tyr Leu Gly
1475 1480 1485
Gln Cys Ala Ala Ile Asn Trp Ala Phe Asp Val His Tyr His Arg
1490 1495 1500
Pro Ser Gly Lys Tyr Gln Met Gly Glu Leu Leu Ser Ser Phe Leu
1505 1510 1515
Ser Arg Met Ser Lys Gly Val Phe Lys Val Leu Val Asn Ala Leu
1520 1525 1530
Ser His Pro Lys Ile Tyr Lys Lys Phe Trp His Cys Gly Ile Ile
1535 1540 1545
Glu Pro Ile His Gly Pro Ser Leu Asp Ala Gln Asn Leu His Thr
1550 1555 1560
Thr Val Cys Asn Met Val Tyr Thr Cys Tyr Met Thr Tyr Leu Asp
1565 1570 1575
Leu Leu Leu Asn Glu Glu Leu Glu Glu Phe Thr Phe Leu Leu Cys
1580 1585 1590
Glu Ser Asp Glu Asp Val Val Pro Asp Arg Phe Asp Asn Ile Gln
1595 1600 1605
Ala Lys His Leu Cys Val Leu Ala Asp Leu Tyr Cys Gln Pro Gly
1610 1615 1620
Thr Cys Pro Pro Ile Arg Gly Leu Arg Pro Val Glu Lys Cys Ala
1625 1630 1635
Val Leu Thr Asp His Ile Lys Ala Glu Ala Arg Leu Ser Pro Ala
1640 1645 1650
Gly Ser Ser Trp Asn Ile Asn Pro Ile Ile Val Asp His Tyr Ser
1655 1660 1665
Cys Ser Leu Thr Tyr Leu Arg Arg Gly Ser Ile Lys Gln Ile Arg
1670 1675 1680
Leu Arg Val Asp Pro Gly Phe Ile Phe Asp Ala Leu Ala Glu Val
1685 1690 1695
Asn Val Ser Gln Pro Lys Val Gly Ser Asp Val Lys Met Asp Lys
1700 1705 1710
Lys Thr Ile Val Trp Phe Arg Arg Asp Leu Arg Ile Glu Asp Asn
1715 1720 1725
Pro Ala Leu Ala Ala Ala Ala His Glu Gly Ser Val Phe Pro Val
1730 1735 1740
Phe Ile Trp Cys Pro Glu Glu Glu Gly Gln Phe Tyr Pro Gly Arg
1745 1750 1755
Ala Ser Arg Trp Trp Met Lys Gln Ser Leu Ala His Leu Ser Gln
1760 1765 1770
Ser Leu Lys Ala Leu Gly Ser Asp Leu Thr Leu Ile Lys Thr His
1775 1780 1785
Asn Thr Ile Ser Ala Ile Leu Asp Cys Ile Arg Val Thr Gly Ala
1790 1795 1800
Thr Lys Val Val Phe Asn His Leu Tyr Asp Pro Val Ser Leu Val
1805 1810 1815
Arg Asp His Thr Val Lys Glu Lys Leu Val Glu Arg Gly Ile Ser
1820 1825 1830
Val Gln Ser Tyr Asn Gly Asp Leu Leu Tyr Glu Pro Trp Glu Ile
1835 1840 1845
Tyr Cys Glu Lys Gly Lys Pro Phe Thr Ser Phe Asn Ser Tyr Trp
1850 1855 1860
Lys Lys Cys Leu Asp Met Ser Ile Glu Ser Val Met Leu Pro Pro
1865 1870 1875
Pro Trp Arg Leu Met Pro Ile Thr Ala Ala Ala Glu Ala Ile Trp
1880 1885 1890
Ala Cys Ser Ile Glu Glu Leu Gly Leu Glu Asn Glu Ala Glu Lys
1895 1900 1905
Pro Ser Asn Ala Leu Leu Thr Arg Ala Trp Ser Pro Gly Trp Ser
1910 1915 1920
Asn Ala Asp Lys Leu Leu Asn Glu Phe Ile Glu Lys Gln Leu Ile
1925 1930 1935
Asp Tyr Ala Lys Asn Ser Lys Lys Val Val Gly Asn Ser Thr Ser
1940 1945 1950
Leu Leu Ser Pro Tyr Leu His Phe Gly Glu Ile Ser Val Arg His
1955 1960 1965
Val Phe Gln Cys Ala Arg Met Lys Gln Ile Ile Trp Ala Arg Asp
1970 1975 1980
Lys Asn Ser Glu Gly Glu Glu Ser Ala Asp Leu Phe Leu Arg Gly
1985 1990 1995
Ile Gly Leu Arg Glu Tyr Ser Arg Tyr Ile Cys Phe Asn Phe Pro
2000 2005 2010
Phe Thr His Glu Gln Ser Leu Leu Ser His Leu Arg Phe Phe Pro
2015 2020 2025
Trp Asp Ala Asp Val Asp Lys Phe Lys Ala Trp Arg Gln Gly Arg
2030 2035 2040
Thr Gly Tyr Pro Leu Val Asp Ala Gly Met Arg Glu Leu Trp Ala
2045 2050 2055
Thr Gly Trp Met His Asn Arg Ile Arg Val Ile Val Ser Ser Phe
2060 2065 2070
Ala Val Lys Phe Leu Leu Leu Pro Trp Lys Trp Gly Met Lys Tyr
2075 2080 2085
Phe Trp Asp Thr Leu Leu Asp Ala Asp Leu Glu Cys Asp Ile Leu
2090 2095 2100
Gly Trp Gln Tyr Ile Ser Gly Ser Ile Pro Asp Gly His Glu Leu
2105 2110 2115
Asp Arg Leu Asp Asn Pro Ala Leu Gln Gly Ala Lys Tyr Asp Pro
2120 2125 2130
Glu Gly Glu Tyr Ile Arg Gln Trp Leu Pro Glu Leu Ala Arg Leu
2135 2140 2145
Pro Thr Glu Trp Ile His His Pro Trp Asp Ala Pro Leu Thr Val
2150 2155 2160
Leu Lys Ala Ser Gly Val Glu Leu Gly Thr Asn Tyr Ala Lys Pro
2165 2170 2175
Ile Val Asp Ile Asp Thr Ala Arg Glu Leu Leu Ala Lys Ala Ile
2180 2185 2190
Ser Arg Thr Arg Glu Ala Gln Ile Met Ile Gly Ala Ala Pro Asp
2195 2200 2205
Glu Ile Val Ala Asp Ser Phe Glu Ala Leu Gly Ala Asn Thr Ile
2210 2215 2220
Lys Glu Pro Gly Leu Cys Pro Ser Val Ser Ser Asn Asp Gln Gln
2225 2230 2235
Val Pro Ser Ala Val Arg Tyr Asn Gly Ser Ala Ala Val Lys Pro
2240 2245 2250
Glu Glu Glu Glu Glu Arg Asp Met Lys Lys Ser Arg Gly Phe Asp
2255 2260 2265
Glu Arg Glu Leu Phe Ser Thr Ala Glu Ser Ser Ser Ser Ser Ser
2270 2275 2280
Val Phe Phe Val Ser Gln Ser Cys Ser Leu Ala Ser Glu Gly Lys
2285 2290 2295
Asn Leu Glu Gly Ile Gln Asp Ser Ser Asp Gln Ile Thr Thr Ser
2300 2305 2310
Leu Gly Lys Asn Gly Cys Lys Thr Gly Asn Ser Ala Asp Gly Gly
2315 2320 2325
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Gly Ser Thr Gln
2330 2335 2340
Gly Gly Ser Met Asn Gly Ala Ile Gly Gly Asp Leu Leu Leu Asn
2345 2350 2355
Phe Pro Asp Met Ser Val Leu Glu Arg Gln Arg Ala His Leu Lys
2360 2365 2370
Tyr Leu Asn Pro Thr Phe Asp Ser Pro Leu Ala Gly Phe Phe Ala
2375 2380 2385
Asp Ser Ser Met Ile Thr Gly Gly Glu Met Asp Ser Tyr Leu Ser
2390 2395 2400
Thr Ala Gly Leu Asn Leu Pro Met Met Tyr Gly Glu Thr Thr Val
2405 2410 2415
Glu Gly Asp Ser Arg Leu Ser Ile Ser Pro Glu Thr Thr Leu Gly
2420 2425 2430
Thr Gly Asn Phe Lys Ala Ala Lys Phe Asp Thr Glu Thr Lys Asp
2435 2440 2445
Cys Asn Glu Ala Ala Lys Lys Met Thr Met Asn Arg Asp Asp Leu
2450 2455 2460
Val Glu Glu Gly Glu Glu Glu Lys Ser Lys Ile Thr Glu Gln Asn
2465 2470 2475
Asn Gly Ser Thr Lys Ser Ile Lys Lys Met Lys His Lys Ala Lys
2480 2485 2490
Lys Glu Glu Asn Asn Phe Ser Asn Asp Ser Ser Lys Val Thr Lys
2495 2500 2505
Glu Leu Glu Lys Thr Asp Tyr Ile Gly Tyr Pro Tyr Asp Val Pro
2510 2515 2520
Asp Tyr Ala Gly Ile Ser Asn Met Ser Ile Lys Asp Phe Arg Pro
2525 2530 2535
Pro His Asp Asp Val Ala Lys Leu Leu Lys Asp Ile Asn Thr Ser
2540 2545 2550
Lys His Asn Leu Pro Ile Ser Gly Gly Ser Leu Ala Asn Tyr Glu
2555 2560 2565
Ile His Ala Phe Arg Arg Ile Gly Leu Asn Ser Ser Ala Cys Tyr
2570 2575 2580
Lys Ala Val Glu Ile Ser Thr Leu Ile Arg Arg Cys Leu Glu Pro
2585 2590 2595
Gly Glu Asp Gly Leu Phe Leu Gly Glu Gly Ser Gly Ser Met Leu
2600 2605 2610
Ile Thr Tyr Lys Glu Ile Leu Lys Leu Asn Lys Cys Phe Tyr Asn
2615 2620 2625
Ser Gly Val Ser Ala Asn Ser Arg Ser Gly Gln Arg Glu Leu Ala
2630 2635 2640
Pro Tyr Pro Ser Glu Val Gly Leu Val Glu His Arg Met Gly Val
2645 2650 2655
Gly Asn Ile Val Lys Val Leu Phe Asn Gly Arg Pro Glu Val Thr
2660 2665 2670
Trp Val Gly Ser Ile Asp Cys Phe Asn Phe Ile Val Ser Asn Ile
2675 2680 2685
Pro Thr Ser Ser Val Gly Phe Ile His Ser Asp Ile Glu Thr Leu
2690 2695 2700
Pro Asn Lys Asp Thr Ile Glu Lys Leu Glu Glu Leu Ala Ala Ile
2705 2710 2715
Leu Ser Met Ala Leu Leu Leu Gly Lys Ile Gly Ser Ile Leu Val
2720 2725 2730
Ile Lys Leu Met Pro Phe Ser Gly Asp Phe Val Gln Gly Phe Ile
2735 2740 2745
Ser Tyr Val Gly Ser His Tyr Arg Glu Val Asn Leu Val Tyr Pro
2750 2755 2760
Arg Tyr Ser Asn Phe Ile Ser Thr Glu Ser Tyr Leu Val Met Thr
2765 2770 2775
Asp Leu Lys Ala Asn Arg Leu Met Asn Pro Glu Lys Ile Lys Gln
2780 2785 2790
Gln Ile Ile Glu Ser Ser Val Arg Thr Ser Pro Gly Leu Ile Gly
2795 2800 2805
His Ile Leu Ser Ile Lys Gln Leu Ser Cys Ile Gln Ala Ile Val
2810 2815 2820
Gly Gly Ala Val Ser Arg Gly Asp Ile Asn Pro Ile Leu Lys Lys
2825 2830 2835
Leu Thr Pro Ile Glu Gln Val Leu Ile Ser Cys Gly Leu Ala Ile
2840 2845 2850
Asn Gly Pro Lys Leu Cys Lys Glu Leu Ile His His Asp Val Ala
2855 2860 2865
Ser Gly Gln Asp Gly Leu Leu Asn Ser Ile Leu Ile Leu Tyr Arg
2870 2875 2880
Glu Leu Ala Arg Phe Lys Asp Asn Gln Arg Ser Gln Gln Gly Met
2885 2890 2895
Phe His Ala Tyr Pro Val Leu Val Ser Ser Arg Gln Arg Glu Leu
2900 2905 2910
Val Ser Arg Ile Thr Arg Lys Phe Trp Gly His Ile Leu Leu Tyr
2915 2920 2925
Ser Gly Asn Arg Lys Leu Ile Asn Arg Phe Ile Gln Asn Leu Lys
2930 2935 2940
Ser Gly Tyr Leu Val Leu Asp Leu His Gln Asn Ile Phe Val Lys
2945 2950 2955
Asn Leu Ser Lys Ser Glu Lys Gln Ile Ile Met Thr Gly Gly Leu
2960 2965 2970
Lys Arg Glu Trp Val Phe Lys Val Thr Val Lys Glu Thr Lys Glu
2975 2980 2985
Trp Tyr Lys Leu Val Gly Tyr Ser Ala Leu Ile Lys Asp
2990 2995 3000
<210> 41
<211> 2887
<212> PRT
<213> Artificial Sequence
<220>
<223> Ln-CRY2PHR-CIB1N-Lc
<400> 41
Met Asp Ser Leu Ser Val Asn Gln Ile Leu Tyr Pro Glu Val His Leu
1 5 10 15
Asp Ser Pro Ile Val Thr Asn Lys Ile Val Ala Ile Leu Glu Tyr Ala
20 25 30
Arg Val Pro His Ala Tyr Ser Leu Glu Asp Pro Thr Leu Cys Gln Asn
35 40 45
Ile Lys His Arg Leu Lys Asn Gly Phe Ser Asn Gln Met Ile Ile Asn
50 55 60
Asn Val Glu Val Gly Asn Val Ile Lys Ser Lys Leu Arg Ser Tyr Pro
65 70 75 80
Ala His Ser His Ile Pro Tyr Pro Asn Cys Asn Gln Asp Leu Phe Asn
85 90 95
Ile Glu Asp Lys Glu Ser Thr Arg Lys Ile Arg Glu Leu Leu Lys Lys
100 105 110
Gly Asn Ser Leu Tyr Ser Lys Val Ser Asp Lys Val Phe Gln Cys Leu
115 120 125
Arg Asp Thr Asn Ser Arg Leu Gly Leu Gly Ser Glu Leu Arg Glu Asp
130 135 140
Ile Lys Glu Lys Ile Ile Asn Leu Gly Val Tyr Met His Ser Ser Gln
145 150 155 160
Trp Phe Glu Pro Phe Leu Phe Trp Phe Thr Val Lys Thr Glu Met Arg
165 170 175
Ser Val Ile Lys Ser Gln Thr His Thr Cys His Arg Arg Arg His Thr
180 185 190
Pro Val Phe Phe Thr Gly Ser Ser Val Glu Leu Leu Ile Ser Arg Asp
195 200 205
Leu Val Ala Ile Ile Ser Lys Glu Ser Gln His Val Tyr Tyr Leu Thr
210 215 220
Phe Glu Leu Val Leu Met Tyr Cys Asp Val Ile Glu Gly Arg Leu Met
225 230 235 240
Thr Glu Thr Ala Met Thr Ile Asp Ala Arg Tyr Ala Glu Leu Leu Gly
245 250 255
Arg Val Arg Tyr Met Trp Lys Leu Ile Asp Gly Phe Phe Pro Ala Leu
260 265 270
Gly Asn Pro Thr Tyr Gln Ile Val Ala Met Leu Glu Pro Leu Ser Leu
275 280 285
Ala Tyr Leu Gln Leu Arg Asp Ile Thr Val Glu Leu Arg Gly Ala Phe
290 295 300
Leu Asn His Cys Phe Thr Glu Ile His Asp Val Leu Asp Gln Asn Gly
305 310 315 320
Phe Ser Asp Glu Gly Thr Tyr His Glu Leu Ile Glu Ala Leu Asp Tyr
325 330 335
Ile Phe Ile Thr Asp Asp Ile His Leu Thr Gly Glu Ile Phe Ser Phe
340 345 350
Phe Arg Ser Phe Gly His Pro Arg Leu Glu Ala Val Thr Ala Ala Glu
355 360 365
Asn Val Arg Lys Tyr Met Asn Gln Pro Lys Val Ile Val Tyr Glu Thr
370 375 380
Leu Met Lys Gly His Ala Ile Phe Cys Gly Ile Ile Ile Asn Gly Tyr
385 390 395 400
Arg Asp Arg His Gly Gly Ser Trp Pro Pro Leu Thr Leu Pro Leu His
405 410 415
Ala Ala Asp Thr Ile Arg Asn Ala Gln Ala Ser Gly Glu Gly Leu Thr
420 425 430
His Glu Gln Cys Val Asp Asn Trp Lys Ser Phe Ala Gly Val Arg Phe
435 440 445
Gly Cys Phe Met Pro Leu Ser Leu Asp Ser Asp Leu Thr Met Tyr Leu
450 455 460
Lys Asp Lys Ala Leu Ala Ala Leu Gln Arg Glu Trp Asp Ser Val Tyr
465 470 475 480
Pro Lys Glu Phe Leu Arg Tyr Asp Pro Pro Lys Gly Thr Gly Ser Arg
485 490 495
Arg Leu Val Asp Val Phe Leu Asn Asp Ser Ser Phe Asp Pro Tyr Asp
500 505 510
Met Ile Met Tyr Val Val Ser Gly Ala Tyr Leu His Asp Pro Glu Phe
515 520 525
Asn Leu Ser Tyr Ser Leu Lys Glu Lys Glu Ile Lys Glu Thr Gly Arg
530 535 540
Leu Phe Ala Lys Met Thr Tyr Lys Met Arg Ala Cys Gln Val Ile Ala
545 550 555 560
Glu Asn Leu Ile Ser Asn Gly Ile Gly Lys Tyr Phe Lys Asp Asn Gly
565 570 575
Met Ala Lys Asp Glu His Asp Leu Thr Lys Ala Leu His Thr Leu Ala
580 585 590
Val Ser Gly Val Pro Lys Asp Leu Lys Glu Ser His Arg Gly Gly Pro
595 600 605
Val Leu Lys Thr Tyr Ser Arg Ser Pro Val His Thr Ser Thr Arg Asn
610 615 620
Val Lys Ala Glu Lys Gly Phe Val Gly Phe Pro His Val Ile Arg Gln
625 630 635 640
Asn Gln Asp Thr Asp His Pro Glu Asn Ile Glu Thr Tyr Glu Thr Val
645 650 655
Ser Ala Phe Ile Thr Thr Asp Leu Lys Lys Tyr Cys Leu Asn Trp Arg
660 665 670
Tyr Glu Thr Ile Ser Leu Phe Ala Gln Arg Leu Asn Glu Ile Tyr Gly
675 680 685
Leu Pro Ser Phe Phe Gln Trp Leu His Lys Arg Leu Glu Thr Ser Val
690 695 700
Leu Tyr Val Ser Asp Pro His Cys Pro Pro Asp Leu Asp Ala His Val
705 710 715 720
Pro Leu Cys Lys Val Pro Asn Asp Gln Ile Phe Ile Lys Tyr Pro Met
725 730 735
Gly Gly Ile Glu Gly Tyr Cys Gln Lys Leu Trp Thr Ile Ser Thr Ile
740 745 750
Pro Tyr Leu Tyr Leu Ala Ala Tyr Glu Ser Arg Val Arg Ile Ala Ser
755 760 765
Leu Val Gln Gly Asp Asn Gln Thr Ile Ala Val Thr Lys Arg Val Pro
770 775 780
Ser Thr Trp His Tyr Asn Leu Lys Lys Arg Glu Ala Ala Arg Val Thr
785 790 795 800
Arg Asp Tyr Phe Val Ile Leu Arg Gln Arg Leu His Asp Ile Gly His
805 810 815
His Leu Lys Ala Asn Glu Thr Ile Val Ser Ser His Phe Phe Val Tyr
820 825 830
Ser Lys Gly Ile Tyr Tyr Asp Gly Leu Leu Val Ser Gln Ser Leu Lys
835 840 845
Ser Ile Ala Arg Cys Val Phe Trp Ser Glu Thr Ile Val Asp Glu Thr
850 855 860
Arg Ala Ala Cys Ser Asn Ile Ala Thr Thr Met Ala Lys Ser Ile Glu
865 870 875 880
Arg Gly Tyr Asp Arg Tyr Leu Ala Tyr Ser Leu Asn Val Leu Lys Val
885 890 895
Ile Gln Gln Ile Leu Ile Ser Leu Gly Phe Thr Ile Asn Ser Thr Met
900 905 910
Thr Arg Asp Val Val Ile Pro Leu Leu Thr Asn Asn Asp Leu Leu Ile
915 920 925
Arg Met Ala Leu Leu Pro Ala Pro Ile Gly Gly Met Asn Tyr Leu Asn
930 935 940
Met Ser Arg Leu Phe Val Arg Asn Ile Gly Asp Pro Val Thr Ser Ser
945 950 955 960
Ile Ala Asp Leu Lys Arg Met Ile Leu Ala Ser Leu Met Pro Glu Glu
965 970 975
Thr Leu His Gln Val Met Thr Gln Gln Pro Gly Asp Ser Ser Phe Leu
980 985 990
Asp Trp Ala Ser Asp Pro Tyr Ser Ala Asn Leu Val Cys Val Gln Ser
995 1000 1005
Ile Thr Arg Leu Leu Lys Asn Ile Thr Ala Arg Phe Val Leu Ile
1010 1015 1020
His Ser Pro Asn Pro Met Leu Lys Gly Leu Phe His Asp Asp Ser
1025 1030 1035
Lys Glu Glu Asp Glu Arg Leu Ala Ala Phe Leu Met Asp Arg His
1040 1045 1050
Ile Ile Val Pro Arg Ala Ala His Glu Ile Leu Asp His Ser Val
1055 1060 1065
Thr Gly Ala Arg Glu Ser Ile Ala Gly Met Leu Asp Thr Thr Lys
1070 1075 1080
Gly Leu Ile Arg Ala Ser Met Arg Lys Gly Gly Leu Thr Ser Arg
1085 1090 1095
Val Ile Thr Arg Leu Ser Asn Tyr Asp Tyr Glu Gln Phe Arg Ala
1100 1105 1110
Gly Met Val Leu Leu Thr Gly Arg Lys Arg Asn Val Leu Ile Asp
1115 1120 1125
Lys Glu Ser Cys Ser Val Gln Leu Ala Arg Ala Leu Arg Ser His
1130 1135 1140
Met Trp Ala Arg Leu Ala Arg Gly Arg Pro Ile Tyr Gly Leu Glu
1145 1150 1155
Val Pro Asp Val Leu Glu Ser Met Arg Gly His Leu Ile Arg Arg
1160 1165 1170
His Glu Thr Cys Val Ile Cys Glu Cys Gly Ser Val Asn Tyr Gly
1175 1180 1185
Trp Phe Phe Val Pro Ser Gly Cys Gln Leu Asp Asp Ile Asp Lys
1190 1195 1200
Glu Thr Ser Ser Leu Arg Val Pro Tyr Ile Gly Ser Thr Thr Asp
1205 1210 1215
Glu Arg Thr Asp Met Lys Leu Ala Phe Val Arg Ala Pro Ser Arg
1220 1225 1230
Ser Leu Arg Ser Ala Val Arg Ile Ala Thr Val Tyr Ser Trp Ala
1235 1240 1245
Tyr Gly Asp Asp Asp Ser Ser Trp Asn Glu Ala Trp Leu Leu Ala
1250 1255 1260
Arg Gln Arg Ala Asn Val Ser Leu Glu Glu Leu Arg Val Ile Thr
1265 1270 1275
Pro Ile Ser Thr Ser Thr Asn Leu Ala His Arg Leu Arg Asp Arg
1280 1285 1290
Ser Thr Gln Val Lys Tyr Ser Gly Thr Ser Leu Val Arg Val Ala
1295 1300 1305
Arg Tyr Thr Thr Ile Ser Asn Asp Asn Leu Ser Phe Val Ile Ser
1310 1315 1320
Asp Lys Lys Val Asp Thr Asn Phe Ile Tyr Gln Gln Gly Met Leu
1325 1330 1335
Leu Gly Leu Gly Val Leu Glu Thr Leu Phe Arg Leu Glu Lys Asp
1340 1345 1350
Thr Gly Ser Ser Asn Thr Val Leu His Leu His Val Glu Thr Asp
1355 1360 1365
Cys Cys Val Ile Pro Met Ile Asp His Pro Arg Ile Pro Ser Ser
1370 1375 1380
Arg Lys Leu Glu Leu Arg Ala Glu Leu Cys Thr Asn Pro Leu Ile
1385 1390 1395
Tyr Asp Asn Ala Pro Leu Ile Asp Arg Asp Ala Thr Arg Leu Tyr
1400 1405 1410
Thr Gln Ser His Arg Arg His Leu Val Glu Phe Val Thr Trp Ser
1415 1420 1425
Thr Pro Gln Leu Tyr His Ile Leu Ala Lys Ser Thr Ala Leu Ser
1430 1435 1440
Met Ile Asp Leu Val Thr Lys Phe Glu Lys Asp His Met Asn Glu
1445 1450 1455
Ile Ser Ala Leu Ile Gly Asp Asp Asp Ile Asn Ser Phe Ile Thr
1460 1465 1470
Glu Phe Leu Leu Ile Glu Pro Arg Leu Phe Thr Ile Tyr Leu Gly
1475 1480 1485
Gln Cys Ala Ala Ile Asn Trp Ala Phe Asp Val His Tyr His Arg
1490 1495 1500
Pro Ser Gly Lys Tyr Gln Met Gly Glu Leu Leu Ser Ser Phe Leu
1505 1510 1515
Ser Arg Met Ser Lys Gly Val Phe Lys Val Leu Val Asn Ala Leu
1520 1525 1530
Ser His Pro Lys Ile Tyr Lys Lys Phe Trp His Cys Gly Ile Ile
1535 1540 1545
Glu Pro Ile His Gly Pro Ser Leu Asp Ala Gln Asn Leu His Thr
1550 1555 1560
Thr Val Cys Asn Met Val Tyr Thr Cys Tyr Met Thr Tyr Leu Asp
1565 1570 1575
Leu Leu Leu Asn Glu Glu Leu Glu Glu Phe Thr Phe Leu Leu Cys
1580 1585 1590
Glu Ser Asp Glu Asp Val Val Pro Asp Arg Phe Asp Asn Ile Gln
1595 1600 1605
Ala Lys His Leu Cys Val Leu Ala Asp Leu Tyr Cys Gln Pro Gly
1610 1615 1620
Thr Cys Pro Pro Ile Arg Gly Leu Arg Pro Val Glu Lys Cys Ala
1625 1630 1635
Val Leu Thr Asp His Ile Lys Ala Glu Ala Arg Leu Ser Pro Ala
1640 1645 1650
Gly Ser Ser Trp Asn Ile Asn Pro Ile Ile Val Asp His Tyr Ser
1655 1660 1665
Cys Ser Leu Thr Tyr Leu Arg Arg Gly Ser Ile Lys Gln Ile Arg
1670 1675 1680
Leu Arg Val Asp Pro Gly Phe Ile Phe Asp Ala Leu Ala Glu Val
1685 1690 1695
Asn Val Ser Gln Pro Lys Val Gly Ser Asp Val Lys Met Asp Lys
1700 1705 1710
Lys Thr Ile Val Trp Phe Arg Arg Asp Leu Arg Ile Glu Asp Asn
1715 1720 1725
Pro Ala Leu Ala Ala Ala Ala His Glu Gly Ser Val Phe Pro Val
1730 1735 1740
Phe Ile Trp Cys Pro Glu Glu Glu Gly Gln Phe Tyr Pro Gly Arg
1745 1750 1755
Ala Ser Arg Trp Trp Met Lys Gln Ser Leu Ala His Leu Ser Gln
1760 1765 1770
Ser Leu Lys Ala Leu Gly Ser Asp Leu Thr Leu Ile Lys Thr His
1775 1780 1785
Asn Thr Ile Ser Ala Ile Leu Asp Cys Ile Arg Val Thr Gly Ala
1790 1795 1800
Thr Lys Val Val Phe Asn His Leu Tyr Asp Pro Val Ser Leu Val
1805 1810 1815
Arg Asp His Thr Val Lys Glu Lys Leu Val Glu Arg Gly Ile Ser
1820 1825 1830
Val Gln Ser Tyr Asn Gly Asp Leu Leu Tyr Glu Pro Trp Glu Ile
1835 1840 1845
Tyr Cys Glu Lys Gly Lys Pro Phe Thr Ser Phe Asn Ser Tyr Trp
1850 1855 1860
Lys Lys Cys Leu Asp Met Ser Ile Glu Ser Val Met Leu Pro Pro
1865 1870 1875
Pro Trp Arg Leu Met Pro Ile Thr Ala Ala Ala Glu Ala Ile Trp
1880 1885 1890
Ala Cys Ser Ile Glu Glu Leu Gly Leu Glu Asn Glu Ala Glu Lys
1895 1900 1905
Pro Ser Asn Ala Leu Leu Thr Arg Ala Trp Ser Pro Gly Trp Ser
1910 1915 1920
Asn Ala Asp Lys Leu Leu Asn Glu Phe Ile Glu Lys Gln Leu Ile
1925 1930 1935
Asp Tyr Ala Lys Asn Ser Lys Lys Val Val Gly Asn Ser Thr Ser
1940 1945 1950
Leu Leu Ser Pro Tyr Leu His Phe Gly Glu Ile Ser Val Arg His
1955 1960 1965
Val Phe Gln Cys Ala Arg Met Lys Gln Ile Ile Trp Ala Arg Asp
1970 1975 1980
Lys Asn Ser Glu Gly Glu Glu Ser Ala Asp Leu Phe Leu Arg Gly
1985 1990 1995
Ile Gly Leu Arg Glu Tyr Ser Arg Tyr Ile Cys Phe Asn Phe Pro
2000 2005 2010
Phe Thr His Glu Gln Ser Leu Leu Ser His Leu Arg Phe Phe Pro
2015 2020 2025
Trp Asp Ala Asp Val Asp Lys Phe Lys Ala Trp Arg Gln Gly Arg
2030 2035 2040
Thr Gly Tyr Pro Leu Val Asp Ala Gly Met Arg Glu Leu Trp Ala
2045 2050 2055
Thr Gly Trp Met His Asn Arg Ile Arg Val Ile Val Ser Ser Phe
2060 2065 2070
Ala Val Lys Phe Leu Leu Leu Pro Trp Lys Trp Gly Met Lys Tyr
2075 2080 2085
Phe Trp Asp Thr Leu Leu Asp Ala Asp Leu Glu Cys Asp Ile Leu
2090 2095 2100
Gly Trp Gln Tyr Ile Ser Gly Ser Ile Pro Asp Gly His Glu Leu
2105 2110 2115
Asp Arg Leu Asp Asn Pro Ala Leu Gln Gly Ala Lys Tyr Asp Pro
2120 2125 2130
Glu Gly Glu Tyr Ile Arg Gln Trp Leu Pro Glu Leu Ala Arg Leu
2135 2140 2145
Pro Thr Glu Trp Ile His His Pro Trp Asp Ala Pro Leu Thr Val
2150 2155 2160
Leu Lys Ala Ser Gly Val Glu Leu Gly Thr Asn Tyr Ala Lys Pro
2165 2170 2175
Ile Val Asp Ile Asp Thr Ala Arg Glu Leu Leu Ala Lys Ala Ile
2180 2185 2190
Ser Arg Thr Arg Glu Ala Gln Ile Met Ile Gly Ala Ala Thr Gly
2195 2200 2205
Asn Ser Ala Asp Gly Gly Gly Gly Ser Gly Gly Ser Gly Gly Ser
2210 2215 2220
Gly Gly Gly Ser Thr Gln Gly Gly Ser Met Asn Gly Ala Ile Gly
2225 2230 2235
Gly Asp Leu Leu Leu Asn Phe Pro Asp Met Ser Val Leu Glu Arg
2240 2245 2250
Gln Arg Ala His Leu Lys Tyr Leu Asn Pro Thr Phe Asp Ser Pro
2255 2260 2265
Leu Ala Gly Phe Phe Ala Asp Ser Ser Met Ile Thr Gly Gly Glu
2270 2275 2280
Met Asp Ser Tyr Leu Ser Thr Ala Gly Leu Asn Leu Pro Met Met
2285 2290 2295
Tyr Gly Glu Thr Thr Val Glu Gly Asp Ser Arg Leu Ser Ile Ser
2300 2305 2310
Pro Glu Thr Thr Leu Gly Thr Gly Asn Phe Lys Ala Ala Lys Phe
2315 2320 2325
Asp Thr Glu Thr Lys Asp Cys Asn Glu Ala Ala Lys Lys Met Thr
2330 2335 2340
Met Asn Arg Asp Asp Leu Val Glu Glu Gly Glu Glu Glu Lys Ser
2345 2350 2355
Lys Ile Thr Glu Gln Asn Asn Gly Ser Thr Lys Ser Ile Lys Lys
2360 2365 2370
Met Lys His Lys Ala Lys Lys Glu Glu Asn Asn Phe Ser Asn Asp
2375 2380 2385
Ser Ser Lys Val Thr Lys Glu Leu Glu Lys Thr Asp Tyr Ile Gly
2390 2395 2400
Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Ile Ser Asn Met Ser
2405 2410 2415
Ile Lys Asp Phe Arg Pro Pro His Asp Asp Val Ala Lys Leu Leu
2420 2425 2430
Lys Asp Ile Asn Thr Ser Lys His Asn Leu Pro Ile Ser Gly Gly
2435 2440 2445
Ser Leu Ala Asn Tyr Glu Ile His Ala Phe Arg Arg Ile Gly Leu
2450 2455 2460
Asn Ser Ser Ala Cys Tyr Lys Ala Val Glu Ile Ser Thr Leu Ile
2465 2470 2475
Arg Arg Cys Leu Glu Pro Gly Glu Asp Gly Leu Phe Leu Gly Glu
2480 2485 2490
Gly Ser Gly Ser Met Leu Ile Thr Tyr Lys Glu Ile Leu Lys Leu
2495 2500 2505
Asn Lys Cys Phe Tyr Asn Ser Gly Val Ser Ala Asn Ser Arg Ser
2510 2515 2520
Gly Gln Arg Glu Leu Ala Pro Tyr Pro Ser Glu Val Gly Leu Val
2525 2530 2535
Glu His Arg Met Gly Val Gly Asn Ile Val Lys Val Leu Phe Asn
2540 2545 2550
Gly Arg Pro Glu Val Thr Trp Val Gly Ser Ile Asp Cys Phe Asn
2555 2560 2565
Phe Ile Val Ser Asn Ile Pro Thr Ser Ser Val Gly Phe Ile His
2570 2575 2580
Ser Asp Ile Glu Thr Leu Pro Asn Lys Asp Thr Ile Glu Lys Leu
2585 2590 2595
Glu Glu Leu Ala Ala Ile Leu Ser Met Ala Leu Leu Leu Gly Lys
2600 2605 2610
Ile Gly Ser Ile Leu Val Ile Lys Leu Met Pro Phe Ser Gly Asp
2615 2620 2625
Phe Val Gln Gly Phe Ile Ser Tyr Val Gly Ser His Tyr Arg Glu
2630 2635 2640
Val Asn Leu Val Tyr Pro Arg Tyr Ser Asn Phe Ile Ser Thr Glu
2645 2650 2655
Ser Tyr Leu Val Met Thr Asp Leu Lys Ala Asn Arg Leu Met Asn
2660 2665 2670
Pro Glu Lys Ile Lys Gln Gln Ile Ile Glu Ser Ser Val Arg Thr
2675 2680 2685
Ser Pro Gly Leu Ile Gly His Ile Leu Ser Ile Lys Gln Leu Ser
2690 2695 2700
Cys Ile Gln Ala Ile Val Gly Gly Ala Val Ser Arg Gly Asp Ile
2705 2710 2715
Asn Pro Ile Leu Lys Lys Leu Thr Pro Ile Glu Gln Val Leu Ile
2720 2725 2730
Ser Cys Gly Leu Ala Ile Asn Gly Pro Lys Leu Cys Lys Glu Leu
2735 2740 2745
Ile His His Asp Val Ala Ser Gly Gln Asp Gly Leu Leu Asn Ser
2750 2755 2760
Ile Leu Ile Leu Tyr Arg Glu Leu Ala Arg Phe Lys Asp Asn Gln
2765 2770 2775
Arg Ser Gln Gln Gly Met Phe His Ala Tyr Pro Val Leu Val Ser
2780 2785 2790
Ser Arg Gln Arg Glu Leu Val Ser Arg Ile Thr Arg Lys Phe Trp
2795 2800 2805
Gly His Ile Leu Leu Tyr Ser Gly Asn Arg Lys Leu Ile Asn Arg
2810 2815 2820
Phe Ile Gln Asn Leu Lys Ser Gly Tyr Leu Val Leu Asp Leu His
2825 2830 2835
Gln Asn Ile Phe Val Lys Asn Leu Ser Lys Ser Glu Lys Gln Ile
2840 2845 2850
Ile Met Thr Gly Gly Leu Lys Arg Glu Trp Val Phe Lys Val Thr
2855 2860 2865
Val Lys Glu Thr Lys Glu Trp Tyr Lys Leu Val Gly Tyr Ser Ala
2870 2875 2880
Leu Ile Lys Asp
2885
<210> 42
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> 锤头型核酶
<400> 42
ctgatgaggc cgaaaggccg aaactccgta aggagtc 37
<210> 43
<211> 109
<212> DNA
<213> Artificial Sequence
<220>
<223> Measles virus基因组5'末端序列
<400> 43
accagacaaa gctgggaata gaaacttcgt attttcaaag ttttctttaa tatattgcaa 60
ataatgccta accacctagg gcaggattag ggttccggag ttcaaccaa 109
<210> 44
<211> 600
<212> DNA
<213> Artificial Sequence
<220>
<223> nano luc序列
<400> 44
ttacgccaga atgcgttcgc acagccgcca gccggtcact ccgttgatgg ttactcggaa 60
cagcagggag ccgtcggggt tgatcaggcg ctcgtcgata attttgttgc cgttccacag 120
ggtccctgtt acagtgatct ttttgccgtc gaacacggcg atgccttcat acggccgtcc 180
gaaatagtcg atcatgttcg gcgtaacccc gtcgattacc agtgtgccat agtgcaggat 240
caccttaaag tgatgatcat ccacagggta caccacctta aaaatttttt cgatctggcc 300
catttggtcg ccgctcagac cttcatacgg gatgatgaca tggatgtcga tcttcagccc 360
attttcaccg ctcaggacaa tcctttggat cggagttacg gacaccccga gattctgaaa 420
caaactggac acacctccct gttcaaggac ttggtccagg ttgtagccgg ctgtctgtcg 480
ccagtcccca acgaaatctt cgagtgtgaa gactggggca gggaaggcag caggcaacac 540
caggagcagg cccagggaga aggcaactgg accgaaggcg cttgtggaga aggagttcat 600
<210> 45
<211> 107
<212> DNA
<213> Artificial Sequence
<220>
<223> Measles virus基因组3'末端序列
<400> 45
ctcggatatc cctaatcctg ctcttgtccc tgataatagg atcttgaatc ctaagtgcac 60
tagaagatga tcattgattg aactatcctt acccaacttt gtttggt 107
<210> 46
<211> 82
<212> DNA
<213> Artificial Sequence
<220>
<223> HDV 核酶序列
<400> 46
gggtcggcat ggcatctcca cctcctcgcg gtccgacctg ggcatccgaa ggagacgtcg 60
tccactcgga tggctaaggg ag 82
<210> 47
<211> 7286
<212> DNA
<213> Artificial Sequence
<220>
<223> Measles virus微型基因组表达质粒
<400> 47
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgctagctt tgcaaagatg gataaagttt taaacagaga 240
ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 300
cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 360
gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 420
actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 480
tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 540
cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 600
ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 660
ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 720
ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 780
ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 840
ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 900
acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 960
cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1020
cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1080
cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1140
cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1200
tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1260
gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1320
acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1380
aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1440
gtttttttct tccatttcag gtgtcgtgag gaattctcta ggcttggtac cgagctcgga 1500
tcccctgtaa tacgactcac tatagggtgt ctggtctgat gaggccgaaa ggccgaaact 1560
ccgtaaggag tcaccagaca aagctgggaa tagaaacttc gtattttcaa agttttcttt 1620
aatatattgc aaataatgcc taaccaccta gggcaggatt agggttccgg agttcaacca 1680
attacgccag aatgcgttcg cacagccgcc agccggtcac tccgttgatg gttactcgga 1740
acagcaggga gccgtcgggg ttgatcaggc gctcgtcgat aattttgttg ccgttccaca 1800
gggtccctgt tacagtgatc tttttgccgt cgaacacggc gatgccttca tacggccgtc 1860
cgaaatagtc gatcatgttc ggcgtaaccc cgtcgattac cagtgtgcca tagtgcagga 1920
tcaccttaaa gtgatgatca tccacagggt acaccacctt aaaaattttt tcgatctggc 1980
ccatttggtc gccgctcaga ccttcatacg ggatgatgac atggatgtcg atcttcagcc 2040
cattttcacc gctcaggaca atcctttgga tcggagttac ggacaccccg agattctgaa 2100
acaaactgga cacacctccc tgttcaagga cttggtccag gttgtagccg gctgtctgtc 2160
gccagtcccc aacgaaatct tcgagtgtga agactggggc agggaaggca gcaggcaaca 2220
ccaggagcag gcccagggag aaggcaactg gaccgaaggc gcttgtggag aaggagttca 2280
tctcggatat ccctaatcct gctcttgtcc ctgataatag gatcttgaat cctaagtgca 2340
ctagaagatg atcattgatt gaactatcct tacccaactt tgtttggtgg gtcggcatgg 2400
catctccacc tcctcgcggt ccgacctggg catccgaagg agacgtcgtc cactcggatg 2460
gctaagggag actagtccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg 2520
gtcttgaggg gttttttgct gaaaggttta aactataaac actagtccgc tgagcaataa 2580
ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga 2640
actatatccg ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 2700
cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaagc ttactagtaa 2760
cggccgccag tgtgctggaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg 2820
catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg 2880
actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 2940
ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 3000
ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 3060
tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 3120
agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 3180
gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 3240
cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 3300
aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 3360
cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 3420
ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 3480
aaccctatct cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg 3540
ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 3600
agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat 3660
ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3720
caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3780
cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3840
tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt 3900
tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct 3960
gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 4020
tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 4080
tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 4140
accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg 4200
gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 4260
tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 4320
gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 4380
tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 4440
ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 4500
ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 4560
gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 4620
cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 4680
gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 4740
tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt 4800
tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 4860
ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 4920
agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 4980
atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5040
attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 5100
cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 5160
cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 5220
aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 5280
acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 5340
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 5400
gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 5460
caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 5520
tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 5580
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 5640
ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 5700
cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 5760
tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 5820
tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 5880
cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 5940
agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 6000
agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 6060
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 6120
aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 6180
ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 6240
gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 6300
taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 6360
tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 6420
tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 6480
gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 6540
gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 6600
ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 6660
cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 6720
tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 6780
cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 6840
agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 6900
cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 6960
aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 7020
aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 7080
gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 7140
gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 7200
tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 7260
ttccccgaaa agtgccacct gacgtc 7286
<210> 48
<211> 6515
<212> DNA
<213> Artificial Sequence
<220>
<223> pCA7-IC-N
<400> 48
gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 60
gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 120
ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 180
ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 240
atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 300
cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 360
tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 420
atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 480
gcgatggggg cggggggggg ggggccgcgc gccagccggg gcggggcggg gcgaggggcg 540
gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 600
tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 660
gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 720
ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 780
gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 840
ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 900
tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 960
cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1020
ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1080
tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1140
cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1200
gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1260
ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1320
gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1380
gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct 1440
ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct 1500
tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg 1560
ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg 1620
cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg 1680
caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattattaa tacgactcac 1740
tatagggaga cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc 1800
tggaattctg gctaatggcc acacttttga ggagcttagc attgttcaaa agaaacaagg 1860
acaaaccacc cattacatca ggatccggtg gagccatcag aggaatcaaa cacattatta 1920
tagtaccaat tcctggagat tcctcaatta ccactcgatc cagactactg gaccggttgg 1980
tcaggttaat tggaaacccg gatgtgagcg ggcccaaact aacaggggca ctaataggta 2040
tattatcctt atttgtggag tctccaggtc aattgattca gaggatcacc gatgaccctg 2100
acgttagcat caggctgtta gaggttgttc agagtgacca gtcacaatct ggccttacct 2160
tcgcatcaag aggtaccaac atggaggatg aggcggacca atacttttca catgatgatc 2220
caagcagtag tgatcaatcc aggtccggat ggttcgagaa caaggaaatc tcagatattg 2280
aagtgcaaga ccctgaggga ttcaacatga ttctgggtac cattctagcc cagatctggg 2340
tcttgctcgc aaaggcggtt acggccccag acacggcagc tgattcggag ctaagaaggt 2400
ggataaagta cacccaacaa agaagggtag ttggtgaatt tagattggag agaaaatggt 2460
tggatgtggt gaggaacagg attgccgagg acctctcttt acgccgattc atggtggctc 2520
taatcctgga tatcaagagg acacccggga acaaacctag gattgctgaa atgatatgtg 2580
acattgatac atatatcgta gaggcaggat tagccagttt tatcctgact attaagtttg 2640
ggatagaaac tatgtatcct gctcttggac tgcatgaatt tgctggtgag ttatccacac 2700
ttgagtcctt gatgaatctt taccagcaaa tgggagaaac tgcaccctac atggtaatcc 2760
tagagaactc aattcagaac aagttcagtg caggatcata ccctctgctc tggagctatg 2820
ccatgggagt aggagtggaa cttgaaaact ccatgggagg tttgaacttt ggtcgatctt 2880
actttgatcc agcatatttt agattagggc aagagatggt gaggaggtca gctggaaagg 2940
tcagttccac attggcatcc gaactcggta tcactgccga ggatgcaagg cttgtttcag 3000
agattgcaat gcatactact gaggacagga tcagtagagc ggtcggaccc agacaagccc 3060
aagtgtcatt tctacacggt gatcaaagtg agaatgagct accaggattg gggggcaagg 3120
aagataggag ggtcaaacag ggtcggggag aagccaggga gagctacaga gaaaccgggt 3180
ccagcagagc aagtgatgcg agagctgccc atcctccaac cagcatgccc ctagacattg 3240
acactgcatc ggagtcaggc caagatccgc aggacagtcg aaggtcagct gacgccctgc 3300
tcaggctgca agccatggca ggaatcttgg aagaacaagg ctcagacacg gacaccccta 3360
gggtatacaa tgacagagat cttctagact aggtgcgaga gatccatcac actggcggcc 3420
gctcgagcat gcatctagag gatctttgtg aaggaaaatt cactcctcag gtgcaggctg 3480
cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc 3540
tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg 3600
gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac 3660
tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga 3720
gtttggcaac atatgcccat atgctggctg ccatgaacaa aggtggctat aaagaggtca 3780
tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 3840
aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 3900
ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 3960
tgtccctctt ctcttatgaa gatccctcga cctgcagccc aagcttggcg taatcatggt 4020
catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 4080
gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 4140
tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgatctcaat 4200
tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 4260
tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 4320
gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 4380
tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 4440
caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 4500
tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag 4560
aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 4620
cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 4680
atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 4740
taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 4800
aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 4860
tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 4920
gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct 4980
cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 5040
cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 5100
atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 5160
tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 5220
ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 5280
acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 5340
aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 5400
aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 5460
tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 5520
cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 5580
catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 5640
ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 5700
aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 5760
ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 5820
caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 5880
attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 5940
agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 6000
actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 6060
ttctgtgact ggtgagtact caaccaagtc attctgagaa tggtgtatgc ggcgaccgag 6120
ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 6180
gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 6240
atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 6300
cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 6360
gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 6420
gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 6480
ggttccgcgc acatttcccc gaaaagtgcc acctg 6515
<210> 49
<211> 6453
<212> DNA
<213> Artificial Sequence
<220>
<223> pCA7-IC-PdeltaC
<400> 49
gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 60
gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 120
ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 180
ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 240
atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 300
cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 360
tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 420
atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 480
gcgatggggg cggggggggg ggggccgcgc gccagccggg gcggggcggg gcgaggggcg 540
gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 600
tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 660
gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 720
ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 780
gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 840
ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 900
tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 960
cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1020
ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1080
tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1140
cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1200
gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1260
ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1320
gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1380
gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct 1440
ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct 1500
tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg 1560
ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg 1620
cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg 1680
caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattattaa tacgactcac 1740
tatagggaga cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc 1800
tggaattccg atggcagaag agcaggcacg ccatgtgaaa aacggactag aatgcatccg 1860
ggctctcaag gccgagccca tcggctcact ggccgtcgag gaagccatgg cagcatggtc 1920
agaaatatca gacaacccag gacaggaccg agccacctgc aaggaagagg aggcaggcag 1980
ttcgggtctc agcaaaccat gcctctcagc aattggatca actgaaggcg gtgcacctcg 2040
catccgcggt cagggatctg gagaaagcga tgacgacgct gaaactttgg gaatcccctc 2100
aagaaatctc caggcatcaa gcactgggtt acagtgttat catgtttatg atcacagcgg 2160
tgaagcggtt aagggaatcc aagatgctga ctctatcatg gttcaatcag gccttgatgg 2220
tgatagcacc ctctcaggag gagacgatga atctgaaaac agcgatgtgg atattggcga 2280
acctgatacc gagggatatg ctatcactga ccggggatct gctcccatct ctatggggtt 2340
cagggcttct gatgttgaaa ctgcagaagg aggggagatc cacgagctcc tgaaactcca 2400
atccagaggc aacaactttc cgaagcttgg gaaaactctc aatgttcctc cgcccccgaa 2460
ccccagtagg gccagcactt ccgagacacc cattaaaaag ggcacagacg cgagattggc 2520
ctcatttgga acggagatcg cgtctttatt gacaggtggt gcaacccaat gtgctcgaaa 2580
gtcaccctcg gaaccatcag ggccaggtgc acctgcgggg aatgtccccg agtgtgtgag 2640
caatgccgca ctgatacagg agtggacacc cgaatctggt accacaatct ccccgagatc 2700
ccagaataat gaagaagggg gagactatta tgatgatgag ctgttctccg atgtccaaga 2760
catcaaaaca gccttggcca aaatacacga ggataatcag aagataatct ccaagctaga 2820
atcattgctg ttattgaagg gagaagttga gtcaattaag aagcagatca acaggcaaaa 2880
tatcagcata tccaccctgg aaggacacct ctcaagcatc atgattgcca ttcctggact 2940
tgggaaggat cccaacgacc ccactgcaga tgtcgaactc aatcccgacc tgaaacccat 3000
cataggcaga gattcaggcc gagcactggc cgaagttctc aagaagcccg ttgccagccg 3060
acaactccag ggaatgacta atggacggac cagttccaga ggacagctgc tgaaggaatt 3120
tcaactaaag ccgatcggga aaaaggtgag ctcagccgtc gggtttgttc ctgacaccgg 3180
ccctgcatca cgcagtgtaa tccgctccat tataaaatcc agccggctag aggaggatcg 3240
gaagcgttac ctgatgactc tccttgatga tatcaaagga gccaacgatc ttgccaagtt 3300
ccaccagatg ctgatgaaga taataatgaa gtagctacag ctcaacttac ctgcggccgc 3360
tcgagcatgc atctagagga tctttgtgaa ggaaaattca ctcctcaggt gcaggctgcc 3420
tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt 3480
tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc 3540
taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc 3600
ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt 3660
ttggcaacat atgcccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc 3720
agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag 3780
gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt 3840
ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg 3900
tccctcttct cttatgaaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca 3960
tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 4020
agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 4080
cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg atctcaatta 4140
gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 4200
cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 4260
ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 4320
caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 4380
aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 4440
aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag 4500
gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4560
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4620
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4680
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4740
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4800
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4860
ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca 4920
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4980
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5040
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5100
cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 5160
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5220
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5280
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5340
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5400
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 5460
gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 5520
tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 5580
ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 5640
accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 5700
agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 5760
acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 5820
tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 5880
cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 5940
tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 6000
ctgtgactgg tgagtactca accaagtcat tctgagaatg gtgtatgcgg cgaccgagtt 6060
gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 6120
tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 6180
ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 6240
gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 6300
cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 6360
gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 6420
ttccgcgcac atttccccga aaagtgccac ctg 6453
<210> 50
<211> 11487
<212> DNA
<213> Artificial Sequence
<220>
<223> pCA7-9301B-wtL
<400> 50
gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 60
gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 120
ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 180
ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 240
atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 300
cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 360
tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 420
atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 480
gcgatggggg cggggggggg ggggccgcgc gccagccggg gcggggcggg gcgaggggcg 540
gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 600
tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 660
gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 720
ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 780
gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 840
ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 900
tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 960
cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1020
ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1080
tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1140
cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1200
gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1260
ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1320
gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1380
gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct 1440
ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct 1500
tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg 1560
ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg 1620
cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg 1680
caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattattaa tacgactcac 1740
tatagggaga cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc 1800
tggaattcat ggactcgcta tctgtcaacc agatcctata ccctgaagtt cacctagata 1860
gcccgatagt taccaataag atagtagcta tcctggagta tgctcgagtc cctcacgctt 1920
acagcctgga ggaccctaca ctgtgtcaga acatcaagca ccgcctaaaa aacggattct 1980
ccaaccaaat gattataaac aatgtggaag ttgggaatgt catcaagtcc aagcttagga 2040
gttatccggc ccactctcat attccatatc caaattgtaa tcaggattta tttaacatag 2100
aagacaaaga gtcaacaagg aagatccgtg agctcctaaa aaagggaaat tcgctgtact 2160
ccaaggtcag tgataaggtt ttccaatgcc tgagggacac taattcacgg cttggcttag 2220
gctctgaatt gagagaggac atcaaggaga aaattattaa cttgggagtt tacatgcaca 2280
gctcccaatg gtttgagccc tttctgtttt ggtttacagt caagactgag atgaggtcag 2340
tgatcaaatc acaaacccat acttgccata ggaggagaca cacacctgta ttcttcactg 2400
gtagttcagt tgagctgtta atctctcgtg accttgttgc tataattagt aaagagtctc 2460
aacatgtata ttacctgacg tttgaactgg ttttgatgta ttgtgatgtc atagagggga 2520
ggttaatgac agagaccgct atgaccattg atgctaggta tgcagaactt ctaggaagag 2580
tcagatacat gtggaaactg atagatggtt tcttccctgc actcgggaac ccaacttatc 2640
aaattgtagc catgctggag ccactttcac ttgcttacct gcaactgagg gatataacag 2700
tagaactcag aggtgctttc cttaaccact gctttactga aatacatgat gttcttgacc 2760
aaaacgggtt ttctgatgaa ggtacttatc atgagttaat tgaagcccta gattacattt 2820
tcataactga tgacatacat ttgacagggg agattttctc atttttcaga agtttcggcc 2880
accccagact tgaagcagta acggctgctg aaaatgttag gaaatacatg aatcagccta 2940
aagtcattgt gtatgagact ctgatgaaag gtcatgccat attttgtgga atcataatca 3000
acggctatcg tgacaggcac ggaggcagtt ggccacccct gaccctcccc ctgcatgctg 3060
cagacacaat ccggaatgcc caagcctcag gcgaagggtt aacacatgag cagtgcgttg 3120
ataactggaa atcatttgct ggagtgagat ttggctgttt tatgcctctt agcctggaca 3180
gtgatctgac aatgtaccta aaggacaagg cacttgctgc tctccaaagg gaatgggatt 3240
cagtttaccc gaaagagttt ctgcgttacg atcctcccaa gggaaccggg tcacggaggc 3300
ttgtagatgt tttccttaat gattcgagct ttgacccata tgatatgata atgtatgtcg 3360
taagtggagc ttacctccat gactctgagt tcaacctgtc ttacagcctg aaagaaaagg 3420
agatcaagga aacaggtaga ctttttgcta aaatgactta caaaatgagg gcatgccaag 3480
tgattgctga aaatctaatc tcaaacggga ttggcaaata ttttaaggac aatgggatgg 3540
ccaaggatga gcacgatttg accaaggcac tccacactct agctgtctca ggagtcccca 3600
aagatctcaa agaaagtcac aggggggggc cagtcttaaa aacctactcc cgaagcccag 3660
tccacacaag taccaggaac gttaaagcag caaaagggtt tgtaggattc cctcatgtaa 3720
ttcgggagaa tcaagacact gatcatccgg agaatataga aacttacgag acagtcagcg 3780
catttatcac gactgatctc aagaagtact gccttaattg gagatatgag accatcagct 3840
tatttgcaca gaggctaaat gagatttacg gattaccctc attttttcag tggctgcata 3900
agaggcttga aacctctgtc ctctatgtaa gtgaccctca ttgccccccc gaccttgacg 3960
cccatgtccc gttatgcaaa gtccctaatg accaaatctt catcaagtac cctatgggag 4020
gcatagaagg gtattgtcag aagttgtgga ccatcagcac cattccctac ttatacctgg 4080
ctgcttatga gagcggagta aggattgctt cgttagtgca aggggacaat cagaccatag 4140
ccgtaacaaa aagggtaccc agcacatggc cttacaacct taagaaacgg gaagctgcta 4200
gagtaactag agattacttt gtaattctta ggcaaaggct acatgacatt ggccatcacc 4260
tcaaggcaaa tgagacaatt gtttcatcac atttttttgt ctattcaaaa ggaatatatt 4320
atgatgggct acttgtgtcc caatcactca agagcatcgc aagatgtgta ttctggtcag 4380
agactatagt tgatgaaaca agggcggcat gcagtaatat tgctacaaca atggccaaaa 4440
gcattgagag aggttatgac cgttatcttg catattccct gaacgtccta aaagtgatac 4500
agcaaatttt gatctccctt ggcttcacaa tcaattcaac catgacccgg gatgtagtca 4560
tacccctcct cacaaacaac gatctcttaa taaggatggc actgttgccc gctcctattg 4620
ggggtatgaa ttatctgaat atgagcaggc tgtttgtcag aaacatcggt gatccagtaa 4680
catcatcaat tgctgatctc aagagaatga ttctcgcatc actaatgcct gaagagaccc 4740
tccatcaagt aatgacacaa caaccggggg actcttcatt cctagactgg gctagcgacc 4800
cttactcagc aaatcttgta tgcgtccaga gcatcactag actcctcaag aacataactg 4860
caaggtttgt cctgatccac agtccaaacc caatgttaaa agggttattc catgatgaca 4920
gcaaagaaga ggacgagaga ctggcggcat tcctcatgga caggcatatt atagtaccta 4980
gggcagctca tgaaatcctg gatcatagtg tcacaggggc aagagagtct attgcaggca 5040
tgctagatac cacaaaaggc ctgattcgag ccagcatgag gaaagggggg ttaacctctc 5100
gagtgataac cagattgtcc aattatgact atgaacaatt tagagcaggg atggtgctat 5160
tgacaggaag aaagagaaat gtcctcattg acaaagagtc atgttcagtt caactggcta 5220
gagccctaag aagccatatg tgggcaagac tagctcgagg acggcctatt tacggccttg 5280
aggtccctga tgtactagaa tctatgcgag gccaccttat tcggcgtcat gagacatgtg 5340
tcatctgcga gtgtggatca gtcaactacg gatggttttt tgtcccctcg ggttgccaac 5400
tggatgatat tgacaaggaa acatcatcct tgagagtccc atatattggt tctaccactg 5460
atgagagaac agacatgaag ctcgccttcg taagagcccc aagtcgatcc ttgcgatctg 5520
ctgttagaat agcaacagtg tactcatggg cttacggtga tgatgatagt tcttggaacg 5580
aagcctggtt gttggcaagg caaagggcca atgtgagcct ggaggagcta agggtgatca 5640
ctcccatctc aacttcgact aatttagcgc ataggttgag ggatcgtagc actcaagtga 5700
aatactcagg tacatccctt gtccgagtgg caaggtatac cacaatctcc aacgacaatc 5760
tctcatttgt catatcagat aagaaggttg atactaactt tatataccaa caaggaatgc 5820
ttctagggtt gggtgtttta gaaacattgt ttcgactcga gaaagatact ggatcatcta 5880
acacggtatt acatcttcac gtcgaaacag attgttgcgt gatcccgatg atagatcatc 5940
ccaggatacc cagctcccgc aagctagagc tgagggcaga gctatgtacc aacccattga 6000
tatatgataa tgcaccttta attgacagag atgcaacaag gctatacacc cagagccata 6060
ggaggcacct tgtggaattt gttacatggt ccacacccca actatatcac attctagcta 6120
agtccacagc actatctatg attgacctgg taacaaaatt tgagaaggac catatgaatg 6180
aaatttcagc tctcataggg gatgacgata tcaatagttt cataactgag tttctgctta 6240
tagagccaag attattcact atctacttgg gccagtgtgc agccatcaat tgggcatttg 6300
atatacatta tcatagacca tcagggaaat atcagatggg tgagctgttg tcttcgttcc 6360
tttctagaat gagcaaagga gtgtttaagg tgcttgtcaa tgctctaagc cacccaaaga 6420
tctacaagaa attctggcac tgtggtatta tagagcctat ccatggtcct tcacttgatg 6480
ctcaaaactt gcacacaact gtgtgcaaca tggtttacac atgctatatg acctacctcg 6540
acctgttgtt gaatgaagag ttagaagagt tcacatttct tttgtgtgaa agcgacgagg 6600
atgtagtacc ggacagattc gacaacatcc aggcaaaaca cttgtgtgtt ctagcagatt 6660
tgtactgtca accagggacc tgcccaccga ttcgaggtct aagaccggta gagaaatgtg 6720
cagttctaac cgatcatatc aaggcagagg ctaggttatc tccagcaggg tcttcgtgga 6780
acataaatcc aattattgta gaccattact catgctctct gacttatctc cggcggggat 6840
ctatcaaaca gataagattg agagttgatc cgggattcat ttttgacgcc ctcgctgagg 6900
taaatgtcag tcaaccaaag atcggcagca acaacatctc aaatatgagc atcaaggatt 6960
tcagacctcc acacgatgat gttgcaaaac tgctcaaaga tatcaacaca agcaagcaca 7020
atcttcccat ttcagggggt agtctcgcca attatgaaat ccatgctttc cgcaggatcg 7080
ggttaaactc atctgcttgc tacaaagctg ttgagatatc aacattaatt aggagatgcc 7140
ttgagccagg ggaagacggc ttgttcttgg gtgaggggtc gggttctatg ttgatcactt 7200
ataaggagat actaaaacta aacaagtgct tctataatag tggggtttcc gccaattcta 7260
gatctggtca aagggaatta gcaccctatc cctccgaagt tggccttgtc gaacacagaa 7320
tgggagtagg taatattgtc aaagtgctct ttaacgggag gcccgaagtc acgtgggtag 7380
gcagtataga ttgcttcaat ttcatagtca gtaacatccc tacctctagt gtggggttta 7440
tccattcaga tatagagacc ttacctaaca aagatactat agagaagcta gaggaattgg 7500
cagccatctt atcgatggct ctgctccttg gcaaaatagg atcaatactg gtgattaagc 7560
ttatgccttt aagcggggat tttgttcagg gatttataag ctatgtaggg tctcattata 7620
gagaagtgaa ccttgtttac cctaggtaca gcaacttcat atctactgaa tcttatttag 7680
ttatgacaga tctcaaagct aaccggctaa taaatcctga aaagattaag cagcagataa 7740
tcgaatcatc tgtgcggact tcacctggac ttataggtca catcctatcc attaagcaac 7800
taagctgcat acaagcaatt gtgggaggcg cagttagtag aggtgatatc aaccctactc 7860
tgaaaaaact tacacctata gagcaggtgc tgatcagttg cgggttgaca attaacggac 7920
ctaaactgtg caaagaatta atccaccatg atgttgcctc agggcaagat ggattgctta 7980
actctatact catcctctac agggagttgg caagattcaa agacaaccaa agaagtcaac 8040
aagggatgtt ccacgcttac cccgtattgg taagtagcag gcaacgagaa cttgtatcta 8100
ggatcacccg caaattttgg gggcatattc ttctttactc cgggaacaga aagttgataa 8160
atcggtttat ccagaatctc aaatccggtt atctaatact agacttacac cagaatatct 8220
tcgttaagaa tctatctaag tcagagaaac agattattat gacggggggt ttaaaacgtg 8280
agtgggtttt taaggtaaca gtcaaggaga ccaaagaatg gtataagttg gtcggataca 8340
gtgccctgat taaggattaa gaattctgca gatatccatc acactggcgg ccgctcgagc 8400
atgcatctag aggatctttg tgaaggaaaa ttcactcctc aggtgcaggc tgcctatcag 8460
aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc 8520
tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa 8580
aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg 8640
acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca 8700
acatatgccc atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata 8760
tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 8820
ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 8880
atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 8940
ttctcttatg aagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 9000
tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 9060
aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 9120
ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgatctca attagtcagc 9180
aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 9240
ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 9300
ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 9360
gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 9420
acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 9480
tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 9540
tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 9600
tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 9660
ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 9720
ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 9780
gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 9840
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 9900
ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 9960
tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 10020
gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 10080
tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 10140
tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 10200
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 10260
ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 10320
ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 10380
gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 10440
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 10500
aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 10560
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 10620
ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 10680
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 10740
ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 10800
ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 10860
ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 10920
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 10980
ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 11040
ctggtgagta ctcaaccaag tcattctgag aatggtgtat gcggcgaccg agttgctctt 11100
gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 11160
ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 11220
cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 11280
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 11340
aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 11400
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 11460
gcacatttcc ccgaaaagtg ccacctg 11487
<210> 51
<211> 12501
<212> DNA
<213> Artificial Sequence
<220>
<223> pCA7-9301B-L-pMag-nMaghigh
<400> 51
gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 60
gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 120
ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 180
ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 240
atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 300
cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 360
tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 420
atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 480
gcgatggggg cggggggggg ggggccgcgc gccagccggg gcggggcggg gcgaggggcg 540
gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 600
tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 660
gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 720
ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 780
gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 840
ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 900
tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 960
cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1020
ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1080
tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1140
cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1200
gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1260
ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1320
gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1380
gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct 1440
ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct 1500
tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg 1560
ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg 1620
cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg 1680
caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattattaa tacgactcac 1740
tatagggaga cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc 1800
tggaattcat ggactcgcta tctgtcaacc agatcctata ccctgaagtt cacctagata 1860
gcccgatagt taccaataag atagtagcta tcctggagta tgctcgagtc cctcacgctt 1920
acagcctgga ggaccctaca ctgtgtcaga acatcaagca ccgcctaaaa aacggattct 1980
ccaaccaaat gattataaac aatgtggaag ttgggaatgt catcaagtcc aagcttagga 2040
gttatccggc ccactctcat attccatatc caaattgtaa tcaggattta tttaacatag 2100
aagacaaaga gtcaacaagg aagatccgtg agctcctaaa aaagggaaat tcgctgtact 2160
ccaaggtcag tgataaggtt ttccaatgcc tgagggacac taattcacgg cttggcttag 2220
gctctgaatt gagagaggac atcaaggaga aaattattaa cttgggagtt tacatgcaca 2280
gctcccaatg gtttgagccc tttctgtttt ggtttacagt caagactgag atgaggtcag 2340
tgatcaaatc acaaacccat acttgccata ggaggagaca cacacctgta ttcttcactg 2400
gtagttcagt tgagctgtta atctctcgtg accttgttgc tataattagt aaagagtctc 2460
aacatgtata ttacctgacg tttgaactgg ttttgatgta ttgtgatgtc atagagggga 2520
ggttaatgac agagaccgct atgaccattg atgctaggta tgcagaactt ctaggaagag 2580
tcagatacat gtggaaactg atagatggtt tcttccctgc actcgggaac ccaacttatc 2640
aaattgtagc catgctggag ccactttcac ttgcttacct gcaactgagg gatataacag 2700
tagaactcag aggtgctttc cttaaccact gctttactga aatacatgat gttcttgacc 2760
aaaacgggtt ttctgatgaa ggtacttatc atgagttaat tgaagcccta gattacattt 2820
tcataactga tgacatacat ttgacagggg agattttctc atttttcaga agtttcggcc 2880
accccagact tgaagcagta acggctgctg aaaatgttag gaaatacatg aatcagccta 2940
aagtcattgt gtatgagact ctgatgaaag gtcatgccat attttgtgga atcataatca 3000
acggctatcg tgacaggcac ggaggcagtt ggccacccct gaccctcccc ctgcatgctg 3060
cagacacaat ccggaatgcc caagcctcag gcgaagggtt aacacatgag cagtgcgttg 3120
ataactggaa atcatttgct ggagtgagat ttggctgttt tatgcctctt agcctggaca 3180
gtgatctgac aatgtaccta aaggacaagg cacttgctgc tctccaaagg gaatgggatt 3240
cagtttaccc gaaagagttt ctgcgttacg atcctcccaa gggaaccggg tcacggaggc 3300
ttgtagatgt tttccttaat gattcgagct ttgacccata tgatatgata atgtatgtcg 3360
taagtggagc ttacctccat gactctgagt tcaacctgtc ttacagcctg aaagaaaagg 3420
agatcaagga aacaggtaga ctttttgcta aaatgactta caaaatgagg gcatgccaag 3480
tgattgctga aaatctaatc tcaaacggga ttggcaaata ttttaaggac aatgggatgg 3540
ccaaggatga gcacgatttg accaaggcac tccacactct agctgtctca ggagtcccca 3600
aagatctcaa agaaagtcac aggggggggc cagtcttaaa aacctactcc cgaagcccag 3660
tccacacaag taccaggaac gttaaagcag caaaagggtt tgtaggattc cctcatgtaa 3720
ttcgggagaa tcaagacact gatcatccgg agaatataga aacttacgag acagtcagcg 3780
catttatcac gactgatctc aagaagtact gccttaattg gagatatgag accatcagct 3840
tatttgcaca gaggctaaat gagatttacg gattaccctc attttttcag tggctgcata 3900
agaggcttga aacctctgtc ctctatgtaa gtgaccctca ttgccccccc gaccttgacg 3960
cccatgtccc gttatgcaaa gtccctaatg accaaatctt catcaagtac cctatgggag 4020
gcatagaagg gtattgtcag aagttgtgga ccatcagcac cattccctac ttatacctgg 4080
ctgcttatga gagcggagta aggattgctt cgttagtgca aggggacaat cagaccatag 4140
ccgtaacaaa aagggtaccc agcacatggc cttacaacct taagaaacgg gaagctgcta 4200
gagtaactag agattacttt gtaattctta ggcaaaggct acatgacatt ggccatcacc 4260
tcaaggcaaa tgagacaatt gtttcatcac atttttttgt ctattcaaaa ggaatatatt 4320
atgatgggct acttgtgtcc caatcactca agagcatcgc aagatgtgta ttctggtcag 4380
agactatagt tgatgaaaca agggcggcat gcagtaatat tgctacaaca atggccaaaa 4440
gcattgagag aggttatgac cgttatcttg catattccct gaacgtccta aaagtgatac 4500
agcaaatttt gatctccctt ggcttcacaa tcaattcaac catgacccgg gatgtagtca 4560
tacccctcct cacaaacaac gatctcttaa taaggatggc actgttgccc gctcctattg 4620
gggggatgaa ttatctgaat atgagcaggc tgtttgtcag aaacatcggt gatccagtaa 4680
catcatcaat tgctgatctc aagagaatga ttctcgcatc actaatgcct gaagagaccc 4740
tccatcaagt aatgacacaa caaccggggg actcttcatt cctagactgg gctagcgacc 4800
cttactcagc aaatcttgta tgcgtccaga gcatcactag actcctcaag aacataactg 4860
caaggtttgt cctgatccac agtccaaacc caatgttaaa agggttattc catgatgaca 4920
gcaaagaaga ggacgagaga ctggcggcat tcctcatgga caggcatatt atagtaccta 4980
gggcagctca tgaaatcctg gatcatagtg tcacaggggc aagagagtct attgcaggca 5040
tgctagatac cacaaaaggc ctgattcgag ccagcatgag gaaggggggg ttaacctctc 5100
gagtgataac cagattgtcc aattatgact atgaacaatt tagagcaggg atggtgctat 5160
tgacaggaag aaagagaaat gtcctcattg acaaagagtc atgttcagtg cagctggcta 5220
gagccctaag aagccatatg tgggcaagac tagctcgagg acggcctatt tacggccttg 5280
aggtccctga tgtactagaa tctatgcgag gccaccttat tcggcgtcat gagacatgtg 5340
tcatctgcga gtgtggatca gtcaactacg gatggttttt tgtcccctcg ggttgccaac 5400
tggatgatat tgacaaggaa acatcatcct tgagagtccc atatattggt tctaccactg 5460
atgagagaac agacatgaag ctcgccttcg taagagcccc aagtagatcc ttgcgatctg 5520
ccgttagaat agcaacagtg tactcatggg cttacggtga tgatgatagc tcttggaacg 5580
aagcctggtt gttggcaagg caaagggcca atgtgagcct ggaggagcta agggtgatca 5640
ctcccatctc gacttcgact aatttagcgc ataggttgag ggatcgtagc actcaagtga 5700
aatactcagg tacatccctt gtccgagtgg caaggtatac cacaatctcc aacgacaatc 5760
tctcatttgt catatcagat aagaaggttg atactaactt tatataccaa caaggaatgc 5820
ttctagggtt gggtgtttta gaaacattgt ttcgactcga gaaagatact ggatcatcta 5880
acacggtatt acatcttcac gtcgaaacag attgttgcgt gatcccgatg atagatcatc 5940
ccaggatacc cagctcccgc aagctagagc tgagggcaga gctatgtacc aacccattga 6000
tatatgataa tgcaccttta attgacagag atgcaacaag gctatacacc cagagccata 6060
ggaggcacct tgtggaattt gttacatggt ccacacccca actatatcac attctagcta 6120
agtccacagc actatctatg attgacctgg taacaaaatt tgagaaggac catatgaatg 6180
aaatttcagc tctcataggg gatgacgata tcaatagttt cataactgag tttctgctta 6240
tagagccaag attattcacc atctacttgg gccagtgtgc agccatcaat tgggcatttg 6300
atgtacatta tcatagacca tcagggaaat atcagatggg tgagctgttg tcttcgttcc 6360
tttctagaat gagcaaagga gtgtttaagg tgcttgtcaa tgctctaagc cacccaaaga 6420
tctacaagaa attctggcat tgtggtatta tagagcctat ccatggtcct tcacttgatg 6480
ctcaaaactt gcacacaact gtgtgcaaca tggtttacac atgctatatg acctacctcg 6540
acctgttgtt gaatgaagag ttagaagagt tcacatttct tttgtgtgaa agcgatgagg 6600
atgtagtacc ggacagattc gacaacatcc aggcaaaaca cttgtgtgtt ctggcagatt 6660
tgtactgtca accagggacc tgcccaccga ttcgaggtct aaggccggta gagaaatgtg 6720
cagttctaac cgatcatatc aaggcagagg ctaggttatc tccagcagga tcttcgtgga 6780
acataaatcc aattattgta gaccattact catgctctct gacttatctc cgtcgaggat 6840
ctatcaaaca gataagattg agagttgatc caggattcat ttttgacgcc ctcgctgagg 6900
taaatgtcag tcagccaaag gtcggcagcg acgtcagcca tactctttat gcccccggtg 6960
gatatgacat tatgggatat ctgaggcaga tcaggaaccg gccaaacccg caggtggaac 7020
tgggccccgt ggatacatcc tgcgccttga ttctttgtga cctgaaacag aaagacaccc 7080
cgatagttta cgcgagtgaa gccttcctct acatgacagg ttacagcaac gcagaggtgc 7140
tgggccggaa ttgccggttt ctgcaaagcc ctgacggcat ggtgaagccc aagagcaccc 7200
ggaagtacgt ggatagtaac acaatcaata ctatgcgcaa ggcaatcgac aggaatgccg 7260
aggtgcaggt tgaagtagtc aattttaaaa agaatggaca gcgatttgtt aatttcctga 7320
ctatgatacc tgttagggac gaaacaggcg agtatcgata ctctatggga ttccagtgcg 7380
aaacagaaac cggtaattcc gctgacggcg gcggaggatc gggtggtagt ggtggttcag 7440
gaggaggatc gacccaagga ggatccatgc atactcttta tgcccccggt ggatatgaca 7500
ttatgggata tctggaccag atcggcaacc ggccaaaccc gcaggtggaa ctgggccccg 7560
tggatacatc ctgcgccttg attctttgtg acctgaaaca gaaagacacc ccgatagttt 7620
acgcgagtga agccttcctc tacatgacag gttacagcaa cgcagaggtg ctgggccgga 7680
attgccggtt tctgcaaagc cctgacggca tggtgaagcc caagagcacc cggaagtacg 7740
tggatagtaa cacaatcaat actatccgca aggcaatcga caggaatgcc gaggtgcagg 7800
ttgaagtagt caattttaaa aagaatggac agcgatttgt taatttcctg actatcatac 7860
ctgttaggga cgaaacaggc gagtatcgat actctatggg attccagtgc gaaacagaat 7920
atccgtacga cgtcccagat tacgcgggca tctcaaatat gagcatcaag gatttcagac 7980
ctccacacga tgatgttgca aaattgctca aagatatcaa cacaagcaag cacaatcttc 8040
ccatttcagg gggtagtctc gccaattatg aaatccatgc tttccgcaga atcgggttaa 8100
actcatctgc ttgctacaaa gctgttgaga tatcaacatt aattaggaga tgccttgagc 8160
caggggaaga cggcttgttc ttgggtgagg ggtcgggttc tatgttgatc acttataagg 8220
agatactaaa actaaacaag tgcttctata atagtggggt ttccgccaat tctagatctg 8280
gtcaaaggga attagcaccc tatccctccg aagttggcct tgtcgaacac agaatgggag 8340
taggtaatat tgtcaaggtg ctctttaacg ggaggcccga agtcacgtgg gtaggcagta 8400
tagattgctt caatttcata gtcagtaata tccctacctc tagtgtgggg tttatccatt 8460
cagatataga gaccttacct aacaaagata ctatagagaa gctagaggaa ttggcagcca 8520
tcttatcgat ggctctgctc cttggcaaaa taggatcaat actggtgatt aagcttatgc 8580
ctttaagcgg ggattttgtt cagggattta taagctatgt agggtctcat tatagagaag 8640
tgaaccttgt ttaccctagg tacagcaact tcatatctac tgaatcttat ttagttatga 8700
cagatctcaa agctaaccgg ctaataaatc ctgaaaagat taagcagcag ataatcgaat 8760
catctgtgcg gacttcacct ggacttatag gtcacatcct atccattaag caactaagct 8820
gcatacaagc aattgtggga ggcgcagtta gtagaggtga tatcaaccct actctgaaaa 8880
aacttacacc tatagagcag gtgctgatca gttgcgggtt gacaattaac ggacctaaac 8940
tgtgcaaaga attaatccac catgatgttg cctcagggca agatggattg cttaactcta 9000
tactcatcct ctacagggag ttggcaagat tcaaagacaa ccaaagaagt caacaaggga 9060
tgttccacgc ttaccccgta ttggtaagta gcaggcaacg agaacttgta tctaggatca 9120
cccgcaaatt ttgggggcat attcttcttt actccgggaa cagaaagttg ataaatcggt 9180
ttatccagaa tctcaaatcc ggttatctaa tactagactt acaccagaat atcttcgtta 9240
agaatctatc taagtcagag aaacagatta ttatgacggg gggtttaaaa cgtgagtggg 9300
tttttaaggt aacagtcaag gagaccaaag aatggtataa gttggtcgga tacagtgccc 9360
tgattaagga ttaagaattc tgcagatatc catcacactg gcggccgctc gagcatgcat 9420
ctagaggatc tttgtgaagg aaaattcact cctcaggtgc aggctgccta tcagaaggtg 9480
gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc 9540
aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa 9600
tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat 9660
gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat 9720
gcccatatgc tggctgccat gaacaaaggt ggctataaag aggtcatcag tatatgaaac 9780
agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt 9840
ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt 9900
tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct 9960
tatgaagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct 10020
gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 10080
aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 10140
gctttccagt cgggaaacct gtcgtgccag cggatccgat ctcaattagt cagcaaccat 10200
agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 10260
gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 10320
gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac 10380
ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 10440
aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 10500
catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 10560
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 10620
gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 10680
caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 10740
tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 10800
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 10860
ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 10920
cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 10980
tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 11040
tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 11100
cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 11160
agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 11220
agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 11280
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 11340
aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 11400
ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 11460
gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 11520
taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 11580
tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 11640
tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 11700
gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 11760
gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 11820
ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 11880
cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 11940
tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 12000
cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 12060
agtactcaac caagtcattc tgagaatggt gtatgcggcg accgagttgc tcttgcccgg 12120
cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 12180
aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 12240
aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 12300
gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 12360
gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 12420
tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 12480
ttccccgaaa agtgccacct g 12501
<210> 52
<211> 15969
<212> DNA
<213> Artificial Sequence
<220>
<223> pHEP+EGFP
<400> 52
acgcttaaca acaaaaccaa agaagaagca gacatcgtca gttgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt gttcaaagtc aataaccggg tggtctcttt 120
gaagcccgag attatcgtgg atcaatatga gtacaagtac cctgctatca aagatttgaa 180
aaagccttgt ataaccctag ggaaagcccc cgatttaaac aaagcttaca aatcagtttt 240
atcaggcatg aatgccgcca aacttgatcc tgatgatgta tgctcctact tggcagcagc 300
aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc 360
acgaaaagga gacaagatca ccccagactc tctagtggag ataaagcgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaagg gaccccactg tctctgaaca 480
tgcatcttta gtcggtcttc tcctgagtct gtacagattg agcaaaatat caggacaaaa 540
cactggtaac tataagacaa acattgcgga tagaatagag cagattttcg agacagcccc 600
ttttgttaag atcgtggaac accacaccct gatgacaact cacaagatgt gtgctaattg 660
gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat 720
cgagcatctg tattcggcaa tcagagtggg cacagttgtc accgcttatg aagactgctc 780
aggactggta tcgtttacag ggttcataaa gcagatcatt ctcaccgcaa gagaagcaat 840
actatatttc ttccacaaga actttgaaga agagataagg agaatgtttg agccagggca 900
agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
gtctccttat tcatcaaatg ccgtcggtca tgtgttcaat ctcattcact ttgttggatg 1020
ctatatgggt caagtcagat ctctaaatgc cacggttatt gctgcatgtg cccctcatga 1080
gatgtctgtt ctagggggct atttgggaga ggagttcttc ggaaaaggga catttgaaag 1140
aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac tgacaaagac 1200
cgacgtggca ctggcagatg acggaactgt caactccgat gacgaggact acttctccgg 1260
tgaaaccaga agtcctgaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa 1320
gagatcacat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgaa tttttaaaca aaacgtattc gagtgactca taaggagttg aatgacaggg 1440
tgccagaaat ccatagattg tgtatatcca tcatgaaaaa aactaacact cctcctttcg 1500
aaccatccca agtatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc 1560
cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca 1620
ggctcatctc cagggagaac ccatagaagt ggacaatctc cctgaggaca tgaggcaatt 1680
tcacctggac gatgaaaaat tgtccaacct tggtgagatg gttagggtgg gcgaaggcaa 1740
gtatcgagag gactttcaga tgaatgaggg agaggacccc aacctcctgt tccaatcgta 1800
cctggacaat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa 1860
gatatggtca cagaccgtag aggaaattat atcctatgtc tcggtcaact ttcctaaccc 1920
tccaggaagg tcttcggagg ataaatcaac ccaaactact ggccgggagc tcaagaagga 1980
gacaacatcc attctttctc aaagagaaag ccaaccttca aaagccggaa tggtggctca 2040
agttgcctct ggccctccat cccttgaatg gtctgccacc aatggagagg atgatctatc 2100
agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc 2160
ttctcgatct tcaggaatat tcttgtataa ttttgagcaa ctggagatga accttgatga 2220
catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa 2280
aatcccccta agatgtgtac tgggatgggt cgctttggcc aactccaaga aattccaatt 2340
gctagtcgag gccgacaagc taagcaaaat catgcaagat gacttggatc gctacacatc 2400
atgctaaccg agtcttcgaa ttcattccct ctagataatg aaaactgaga tgtcatggag 2460
tcgatatgaa aaaaacaggc aacaccacta ataaaatgaa ctttctatgt aagatagtga 2520
aaaactgtag ggatgaggac acccaaaagc cctctcccgc gtcagcccct ccggatggcg 2580
atgacctgtg gcttccacct ccagaatatg tcccgctgaa agaactcaca agcaagaaga 2640
acatgaggaa cttttgtatc aacggggagg ttaaagtgtg cagtccgaac ggttactcat 2700
tcaggatcct gcggcatatt ctgagatcat tcgacgagat atactctggg aatcatagga 2760
tgattgggtt agtcaaagtt gttgttggac tagctttatc aggagctcca gctcctgagg 2820
gcatgaactg ggtatacaaa ttgaggagaa cccttatttt ccagtgggct gattccaggg 2880
gccctcttga aggggaggag ttggaacact ctcaagagat cacttgggac gatgatactg 2940
aattcgtcgg attgcaaatg agagtgagcg caagacaatg tcatattcaa ggcaggatct 3000
ggtgtatcga catgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac 3060
aaaggtctga agaggacaaa gactcttctc tgcttttaga ataatcagat aatatcccgc 3120
aagttcatca cttgtttact tctggaggag agaacatacg ggcttaactc caatccttgg 3180
gagcaataga acaaaaaaac acgttatgat gccattaaac cgctgcattt tatcaaagtc 3240
aagttaatta ccgttacatt ttgagcctct tggatgtgaa aaaaactatt aacatccctc 3300
aaaagactta aggaaagatg gttcctcagg ttcttttgtt tgcacccctc ctggtttctc 3360
cattgtgttt cgggaagttc cccatttaca cgataccaga caaacttggt ccctggagcc 3420
ctattgactt acaccatctc agctgtccaa ataacctggt tgtggaggac gaaggatgta 3480
ccaacctgtc cgggttctct tacatggaac ttaaagtggg atacatctca gccataaaag 3540
tgaacgggtt cacttgcaca ggtgttgtga cagaggcaga aacctacacc aactttgttg 3600
gttatgtcac aaccacattc aagagaaagc atttccgccc caccccagac gcatgtagag 3660
ccgcgtataa ctggaagatg gccggtgacc ccagatatga agagtctcta cacaatccgt 3720
accccgacta ccattggctt cgaactgtaa aaaccaccaa agagtctctc gttatcatat 3780
ccccaagtgt gacagatttg gacccatatg acaaatccct tcactcaagg gtcttccctg 3840
gcggaaattg ctcaggaata acggtgtcct cgacctactg ctcaactaat catgattaca 3900
ccatctggat gcctgagaat ctgagactag ggacatcttg tgacattttt acccatagca 3960
gagggaagag agcatccaaa ggagacaaga cttgcggctt tgtggatgaa agaggcctgt 4020
ataagtcttt aaagggagca tgcaaactca agttatgtgg agttctcgga cttagactta 4080
tggatggaac atgggtcgcg atgcaaacat cagatgagac caaatggtgc cctccaggtc 4140
agttggtgaa tttgcacgac tttcgctcag acgagattga gcatctcgtt gaggaagagt 4200
tagtcaagaa aagagaggag tgtctggatg cactagagtc catcatgacc accaagtcag 4260
tgagtttcag acgtctcagt cacctgagaa aacttgtccc tgggtttgga aaagcatata 4320
ccatattcaa caaaaccttg atggaggctg atgctcacta caagtctgtc cagacctgga 4380
atgagatcat cccctcaaaa gggtgtttga gagttgggga gaggtgtcat ccccatgtga 4440
acggggtgtt tttcaatggt ataatattag ggtctgacgg ccatgttcta atcccagaga 4500
tgcagtcatc cctcctccag caacatatgg agttgttgga atcttcagtt atccccctga 4560
tgcacccctt ggcagaccct tctacagttt tcaaagacgg tgatgaggtt gaggattttg 4620
ttgaagttca cctccccgat gtgcataaac aggtctcagg agttgacctg ggtctcccga 4680
aatgggggaa gtatgtattg atgattgcag gggccttgat tgccctgatg ttgataattt 4740
tcctgatgac atgttgcaga agagtcaatc gaccagaatc tacacaaagc aatcttggag 4800
ggacagggag aaatgtgtca gtcccttccc aaagcggaaa agtcatatct tcatgggagt 4860
catataagag tggaggcgag accagactgt gaaggccggt catccttttg acacctcaag 4920
tccagaggat aacctcctcc cggggaaaaa aactaacacc tctcgtacga ccatggtgag 4980
caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 5040
aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 5100
gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 5160
caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 5220
cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 5280
cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 5340
catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 5400
gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 5460
ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 5520
ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 5580
cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 5640
gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aactgcagtt 5700
tgctagcagg accggtacat aactataaag ggctgggtca tctaagcatt tcagtcgaga 5760
aaaaaactgt ataccaaaag aacaactaac aacacttctc atctcgagac ccatatcaag 5820
atgctggatc cgggagaggt ttatgatgac cctattgatc caattgagtc agaggctgaa 5880
cccagaggaa cccccactgt ccccaacatc ttgaggaact ctgactacaa tctcaactct 5940
cctttgattg aggactctgc caaactaatg ttagaatggt tgaaaacagg gaacagacct 6000
tatcggatga ctttgacaga caattgctcc aggtcttaca aagatttgaa agattatttc 6060
aagaaagtag atttgggttc tctcaaagtg ggaggaactg ctgcacaatc aatgatttct 6120
ctctggttgt atggagccca ctctgagtca aacaggagcc ggagatgtat aaccgacttg 6180
gcccatttct attccaagtc atcccccata gagaagctgt tgaattgtac gttaggaaac 6240
agaggcctga gaatcccacc agagggggtg ttaagttgcc ttgagagggt cgattatgat 6300
aaggcatttg ggaggtatct ggccaacacg cattcctctt atctgttttt ccatgtaatt 6360
accttataca tgaatgccct agactgggaa gaggaaaaaa ccatcctagc attatggaaa 6420
gatctaacct cagtggacac cgggaaggac ttggtcaaat tcaaagatca aatatgggga 6480
ctgctgattg tgacaaagga ctttgtttac tctcagagtt ctaactgtct ttttgacaga 6540
aactatacac tgatgctaaa ggatcttttc ttgtctcgat tcaactcctt gatgattttg 6600
ctttctcccc ctgagccccg atactcagat gacttaatat ctcagctgtg ccagctatac 6660
atcgctgggg atcaagtctt gtccttgtgt gggaactccg gctatgaagt catcaaaata 6720
ttggagccat atgtcgtgaa cagtttggtc cagagggcag agaagtttag gcctctcatc 6780
cactccttgg gagactttcc tatgtttata aaagacaagg tgaatcaact tgaagggact 6840
ttcggtccca gtgcaaaaag gttttttagg gttctagatc aattcgacaa catacatgat 6900
ctagtatttg tgtatggctg ttacagacat tgggggcacc cctatataga ttatcggaag 6960
ggtctgttga aactatatga tcaagttcac attaagaaag taatagataa gtcctaccag 7020
gagtgtttag caagtgactt ggctagaagg atcctcagat ggggatttga caagtactcc 7080
aagtggtatc tagattcgag attccttgcc cgagaccacc ccttggctcc ttatatcaag 7140
acccaaacat ggccacccaa acatatagta gacttggtgg gggacacatg gcacaagctc 7200
ccgatcacgc agatctttga gattcctgaa tcaatggacc cgtcagagat actggatgat 7260
aaatcacatt ctttcaccag aacaagacta gcttcttggc tgtcagagaa ccgagggggg 7320
cctgttccta gcgagaaggt cattatcacg gccctgtctc agccacctgt caatccccga 7380
gagtttttga aatctatcga cctcggagga ttgccagatg atgacttgat aattggcctc 7440
agaccaaagg aacgggagtt gaagattgag ggccgattct tcgctctaat gtcatggaat 7500
ctaagattat attttgtcat caccgagaag ctcttggcca actacatttt gccacttttt 7560
gacgcactga ctatgacaga caacctgaac aaggtattta aaaagttgat cgacagggtc 7620
accgggcaag ggcttttgga ctattcgagg gtcacttacg catttcacct ggactatgag 7680
aaatggaaca atcatcaaag attggagtca acggaggatg tattttctgt cctagatcag 7740
gtgtttggat tgaagagggt gttttctaga acacacgagt tttttcagaa gtcctggatc 7800
tattattcag acagatcaga cctcattggg ttacgggagg atcagatata ttgcttggat 7860
atgtccaatg gtccaacctg ctggaatggc caagacggcg ggctagaggg cttacggcag 7920
aagggctgga gtctagtcag cttattgatg atagatagag aatctcaaac caggaacaca 7980
agaaccaaga tactagctca aggagacaac caggttctgt gtccgacata catgttgtca 8040
ccgggattgt ctcaagaggg gcttctctat gagttagaga gcatatcgag gaatgcactc 8100
tcaatatacc gagctatcga ggaaggagca tctaaactgg ggctgatcat caagaaagaa 8160
gagaccatgt gtagttatga ctttctcata tatgggaaga cccccttatt tcgaggcaac 8220
atattagtac ctgaatccaa aagatgggcc cgagtctctt gcatctctaa cgaccaaata 8280
gtcaacctcg ccaatataat gtctacagta tccaccaatg cgctgacagt ggcacaacac 8340
tctcaatctc tgatcaaacc tatgagggat tttctgctca tgtcagtaca ggcagttttc 8400
cactacctgc tgtttagccc aatcttaaaa ggcagggttt ataagattct gagtgctgaa 8460
ggggagagct ttctcctagc catgtcgcgg ataatctatc tagatccttc tttgggaggg 8520
gtgtctggaa tgtctctcgg gaggttccat atacgtcagt tctcagaccc tgtctctgaa 8580
gggttgtcat tctggagaga gatctggtta agctcccacg aatcctggat tcacgcgttg 8640
tgtcaagagg ccgggaaccc cgatcttgga gagagaacac tagagagctt cactcgcctt 8700
ttagaagatc ctactacctt aaatatcaaa ggaggggcca gtcctaccat tctactcaag 8760
gatgctatca gaaaggctct gtacgacgag gtggacaagg tggaaaattc agagtttcga 8820
gaggcaatcc tgttgtccaa gacccataga gataacttta tactcttttt aaaatctgtt 8880
gagcctctgt tccctcgatt tctcagtgaa ctcttcagtt cgtccttctt gggaatacca 8940
gagtcaatta ttggactgat acaaaactcc aggacaataa gaaggcagtt tagaaagagt 9000
ctctcaagaa ctttagaaga gtccttctac aactcagaga tccacgggat taatcggatg 9060
acccagacac ctcaaagggt cgggagagtg tggccctgct cttcagagag ggcagatcta 9120
cttagggaga tctcttgggg aaggaaagtg gtgggcacga cagttcctca cccttccgag 9180
atgttggggc tgcttccaaa atcctctatt tcctgcactt gtggagcaac agggggaggc 9240
aatcctagag tttctgtatc agtactcccg tccttcgatc agtcattttt ttcacgaggt 9300
cctctaaagg gatacttggg ctcgtccacc tccatgtcaa cccagctatt ccatgcatgg 9360
gaaaaagtca ctaatgttca tgtggtgaaa agggctatat cgttaaaaga atctataaac 9420
tggttcatca atagaaattc caatttggct caaactctaa ttagaaacat catgtctctg 9480
acaggccctg atttctctct agaagaggct cctgttttca aacggacagg gtcagccttg 9540
cataggttca agtctgccag atacagcgaa ggagggtatt cttctgtttg tcctaacctt 9600
ctctctcata tctctgtcag tacagacact atgtctgatt tgacccaaga cgggaagaac 9660
tatgatttca tgtttcagcc attgatgctt tatgcgcaaa catggacatc agagctggta 9720
cagagagaca caagactgag agactccacg tttcactggc accttcgatg caacagatgt 9780
gtaaggccca ttgaggatat aacactggaa acttctcaga tcttcgagtt cccggatgtg 9840
tcaaaaagga tatccaggat ggtttctgga gccgtccctc actttcagaa gcttcctgat 9900
atccgtctaa gaccaggtga ttttgaatct ctaagtggta gagaaaagtc tcgccacata 9960
gggtcagctc aggggctctt atactcaatc ttagtagcaa ttcacgattc aggatacaat 10020
gatgggacca tcttccctgt caacatatac ggcaaggttt cccctagaga ctatttgaga 10080
gggctcgcaa gagggatctt gataggatcc tcgatttgct tcttgacacg aatgacaaat 10140
attaatatta aaagacctct tgaattgatc tcaggggtaa tttcctatat tctcctgagg 10200
ctggataatc atccctctct gtatataatg cttagagaac cgtctcttag aggagagata 10260
ttctctatcc ctcagaagat ccccgccgct tacccaacca ctatgaaaga aggcaacaga 10320
tcgatcttgt gttacctcca acacgtgcta cgctatgagc gagaagtaat cacggcgtcc 10380
ccggagaatg actggctgtg gatcttctca gacttcagaa gtgcgaaaat gacgtacttg 10440
accctcatta cctaccagtc tcacctccta ctccagaggg ttgagcgaaa cttgtctaag 10500
agtatgagag ctactctgca acaaatgggt tccttaatga ggcaagtgct gggtgggcac 10560
ggagaagaca ccttggagtc agacgacgac attcaacgat tactaaaaga ctctttgcga 10620
aggacgaggt gggtagatca agaggtgcgc catgcagcta gaaccatgag tggagattac 10680
agccccaaca agagagtatc tcgcaaggca ggatgttcag aatgggtctg ctctgctcaa 10740
caggttgccg tctccacctc agccaacccg gcccctgtct cagagcttga cattagggcc 10800
ctatctaaga ggtttcaaaa ccccttgatc tcgggcctaa gagtggttca gtgggcaacc 10860
ggcgcccatt ataagctaaa gcctattcta gatgatctaa atgttttccc atctctctgt 10920
cttgttgttg gagacgggtc agggggaata tcaagggcag ttctcaacat gtttccagat 10980
tctaagcttg tgttcaacag cctattggag gtgaatgatc tgatggcttc cggaacacgt 11040
ccactgcctc cttcagcaat catgagtgga ggagatgata tcatctccag agtgatagac 11100
tttgactcaa tctgggaaaa accatccgac ctgaggaact tggccacctg gagatacttc 11160
cagtcagttc aaaagcaggt caacatgtcg tatgacctca ttatttgtga tgcagaagtt 11220
actgatattg catctatcaa ccggataact ctgttgatgt ctgatttcgc attgtctata 11280
gatggaccac tttatctggt cttcaaaact tacgggacta tgctagtgaa cccagactat 11340
aaagctatcc aacatctgtc aagagcgttc ccttcggtca cagggtttat aacccaagta 11400
acttcgtcct tttcttctga gctgtacctc cggttctcca aacgagggaa gttttttagg 11460
gatgctgagt acttgacctc ttccaccctt cgggagatga gccttgtgtt attcaattgt 11520
agcagcccca aaagtgagat gcagagagct cgttccttaa actatcaaga tcttgtaagg 11580
ggatttcctg aagagatcat atcaaatcct tacaatgaaa tgatcataac tctgattgac 11640
agtgatgtag agtccttcct ggtccacaag atggtggatg atcttgagtt acagagggga 11700
actctgtcta aagtggctat cattatatcc atcatgatcg ttttttccaa cagagtcttc 11760
aacatttcca aacctttgac tgaccccttg ttctatcccc catctgatcc taaaatcctg 11820
aggcacttca acatatgttg cagtactatg atgtatctat ctaccgcttt aggcgacgtc 11880
cctagcttcg caagacttca tgacctatat aatagaccta taacttatta cttcagaaag 11940
caagttattc gagggaatat ctatctatct tggagctggt ccgatgacac cccagtgttc 12000
aagagggtag cctgtaattc tagcttgagt ctgtcatctc actggatcag gttgatttac 12060
aagatagtga agactaccag actcgttggc cgcatagaag atctatcagg agaggtagaa 12120
cgacacctgc atgggtacaa cagatggatc accctcgagg atatccgatc tagatcatcc 12180
ctactagact acagttgttt gtaagccgga cattaatgaa agcctgtaca tgctaaaatt 12240
cttgtatgat gcatcttgaa aaaaacaaga tcttgaatca gaacctctgg ttgtttgatt 12300
gtttttttta tctttattgt ttatttgtta agcgtgggtc ggcatggcat ctccacctcc 12360
tcgcggtccg acctgggcat ccgaaggagg acgcacgtcc actcggatgg ctaagggagg 12420
gcgggtacca cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 12480
gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 12540
gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 12600
ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 12660
cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 12720
gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 12780
ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 12840
ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 12900
ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 12960
attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 13020
aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 13080
gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 13140
aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 13200
gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 13260
cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 13320
tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 13380
tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 13440
agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 13500
cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 13560
tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 13620
ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 13680
tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 13740
cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 13800
actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 13860
ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 13920
tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 13980
ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 14040
aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 14100
ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 14160
agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 14220
ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 14280
tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 14340
cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 14400
ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 14460
gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 14520
ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 14580
aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 14640
cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 14700
ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 14760
gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 14820
ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 14880
ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 14940
gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 15000
cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 15060
ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 15120
aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 15180
ttcacacagg aaacagctat gaccatgatt acgccaagcg cgctgtacgg gccagatata 15240
cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 15300
atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 15360
cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 15420
tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag 15480
tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 15540
ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 15600
acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 15660
gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 15720
tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 15780
cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa 15840
ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc 15900
aagctggcta gtgttaagcg tctgatgagt ccgtgaggac gaaactatag gaaaggaatt 15960
cctatagtc 15969
<210> 53
<211> 16992
<212> DNA
<213> Artificial Sequence
<220>
<223> pHEP-pMag-nMaghigh
<400> 53
acgcttaaca acaaaaccaa agaagaagca gacatcgtca gttgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt gttcaaagtc aataaccggg tggtctcttt 120
gaagcccgag attatcgtgg atcaatatga gtacaagtac cctgctatca aagatttgaa 180
aaagccttgt ataaccctag ggaaagcccc cgatttaaac aaagcttaca aatcagtttt 240
atcaggcatg aatgccgcca aacttgatcc tgatgatgta tgctcctact tggcagcagc 300
aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc 360
acgaaaagga gacaagatca ccccagactc tctagtggag ataaagcgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaagg gaccccactg tctctgaaca 480
tgcatcttta gtcggtcttc tcctgagtct gtacagattg agcaaaatat caggacaaaa 540
cactggtaac tataagacaa acattgcgga tagaatagag cagattttcg agacagcccc 600
ttttgttaag atcgtggaac accacaccct gatgacaact cacaagatgt gtgctaattg 660
gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat 720
cgagcatctg tattcggcaa tcagagtggg cacagttgtc accgcttatg aagactgctc 780
aggactggta tcgtttacag ggttcataaa gcagatcatt ctcaccgcaa gagaagcaat 840
actatatttc ttccacaaga actttgaaga agagataagg agaatgtttg agccagggca 900
agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
gtctccttat tcatcaaatg ccgtcggtca tgtgttcaat ctcattcact ttgttggatg 1020
ctatatgggt caagtcagat ctctaaatgc cacggttatt gctgcatgtg cccctcatga 1080
gatgtctgtt ctagggggct atttgggaga ggagttcttc ggaaaaggga catttgaaag 1140
aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac tgacaaagac 1200
cgacgtggca ctggcagatg acggaactgt caactccgat gacgaggact acttctccgg 1260
tgaaaccaga agtcctgaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa 1320
gagatcacat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgaa tttttaaaca aaacgtattc gagtgactca taaggagttg aatgacaggg 1440
tgccagaaat ccatagattg tgtatatcca tcatgaaaaa aactaacact cctcctttcg 1500
aaccatccca agtatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc 1560
cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca 1620
ggctcatctc cagggagaac ccatagaagt ggacaatctc cctgaggaca tgaggcaatt 1680
tcacctggac gatgaaaaat tgtccaacct tggtgagatg gttagggtgg gcgaaggcaa 1740
gtatcgagag gactttcaga tgaatgaggg agaggacccc aacctcctgt tccaatcgta 1800
cctggacaat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa 1860
gatatggtca cagaccgtag aggaaattat atcctatgtc tcggtcaact ttcctaaccc 1920
tccaggaagg tcttcggagg ataaatcaac ccaaactact ggccgggagc tcaagaagga 1980
gacaacatcc attctttctc aaagagaaag ccaaccttca aaagccggaa tggtggctca 2040
agttgcctct ggccctccat cccttgaatg gtctgccacc aatggagagg atgatctatc 2100
agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc 2160
ttctcgatct tcaggaatat tcttgtataa ttttgagcaa ctggagatga accttgatga 2220
catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa 2280
aatcccccta agatgtgtac tgggatgggt cgctttggcc aactccaaga aattccaatt 2340
gctagtcgag gccgacaagc taagcaaaat catgcaagat gacttggatc gctacacatc 2400
atgctaaccg agtcttcgaa ttcattccct ctagataatg aaaactgaga tgtcatggag 2460
tcgatatgaa aaaaacaggc aacaccacta ataaaatgaa ctttctatgt aagatagtga 2520
aaaactgtag ggatgaggac acccaaaagc cctctcccgc gtcagcccct ccggatggcg 2580
atgacctgtg gcttccacct ccagaatatg tcccgctgaa agaactcaca agcaagaaga 2640
acatgaggaa cttttgtatc aacggggagg ttaaagtgtg cagtccgaac ggttactcat 2700
tcaggatcct gcggcatatt ctgagatcat tcgacgagat atactctggg aatcatagga 2760
tgattgggtt agtcaaagtt gttgttggac tagctttatc aggagctcca gctcctgagg 2820
gcatgaactg ggtatacaaa ttgaggagaa cccttatttt ccagtgggct gattccaggg 2880
gccctcttga aggggaggag ttggaacact ctcaagagat cacttgggac gatgatactg 2940
aattcgtcgg attgcaaatg agagtgagcg caagacaatg tcatattcaa ggcaggatct 3000
ggtgtatcga catgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac 3060
aaaggtctga agaggacaaa gactcttctc tgcttttaga ataatcagat aatatcccgc 3120
aagttcatca cttgtttact tctggaggag agaacatacg ggcttaactc caatccttgg 3180
gagcaataga acaaaaaaac acgttatgat gccattaaac cgctgcattt tatcaaagtc 3240
aagttaatta ccgttacatt ttgagcctct tggatgtgaa aaaaactatt aacatccctc 3300
aaaagactta aggaaagatg gttcctcagg ttcttttgtt tgcacccctc ctggtttctc 3360
cattgtgttt cgggaagttc cccatttaca cgataccaga caaacttggt ccctggagcc 3420
ctattgactt acaccatctc agctgtccaa ataacctggt tgtggaggac gaaggatgta 3480
ccaacctgtc cgggttctct tacatggaac ttaaagtggg atacatctca gccataaaag 3540
tgaacgggtt cacttgcaca ggtgttgtga cagaggcaga aacctacacc aactttgttg 3600
gttatgtcac aaccacattc aagagaaagc atttccgccc caccccagac gcatgtagag 3660
ccgcgtataa ctggaagatg gccggtgacc ccagatatga agagtctcta cacaatccgt 3720
accccgacta ccattggctt cgaactgtaa aaaccaccaa agagtctctc gttatcatat 3780
ccccaagtgt gacagatttg gacccatatg acaaatccct tcactcaagg gtcttccctg 3840
gcggaaattg ctcaggaata acggtgtcct cgacctactg ctcaactaat catgattaca 3900
ccatctggat gcctgagaat ctgagactag ggacatcttg tgacattttt acccatagca 3960
gagggaagag agcatccaaa ggagacaaga cttgcggctt tgtggatgaa agaggcctgt 4020
ataagtcttt aaagggagca tgcaaactca agttatgtgg agttctcgga cttagactta 4080
tggatggaac atgggtcgcg atgcaaacat cagatgagac caaatggtgc cctccaggtc 4140
agttggtgaa tttgcacgac tttcgctcag acgagattga gcatctcgtt gaggaagagt 4200
tagtcaagaa aagagaggag tgtctggatg cactagagtc catcatgacc accaagtcag 4260
tgagtttcag acgtctcagt cacctgagaa aacttgtccc tgggtttgga aaagcatata 4320
ccatattcaa caaaaccttg atggaggctg atgctcacta caagtctgtc cagacctgga 4380
atgagatcat cccctcaaaa gggtgtttga gagttgggga gaggtgtcat ccccatgtga 4440
acggggtgtt tttcaatggt ataatattag ggtctgacgg ccatgttcta atcccagaga 4500
tgcagtcatc cctcctccag caacatatgg agttgttgga atcttcagtt atccccctga 4560
tgcacccctt ggcagaccct tctacagttt tcaaagacgg tgatgaggtt gaggattttg 4620
ttgaagttca cctccccgat gtgcataaac aggtctcagg agttgacctg ggtctcccga 4680
aatgggggaa gtatgtattg atgattgcag gggccttgat tgccctgatg ttgataattt 4740
tcctgatgac atgttgcaga agagtcaatc gaccagaatc tacacaaagc aatcttggag 4800
ggacagggag aaatgtgtca gtcccttccc aaagcggaaa agtcatatct tcatgggagt 4860
catataagag tggaggcgag accagactgt gaaggccggt catccttttg acacctcaag 4920
tccagaggat aacctcctcc cggggaaaaa aactaacacc tctcgtacga ccatggtgag 4980
caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 5040
aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 5100
gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 5160
caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 5220
cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 5280
cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 5340
catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 5400
gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 5460
ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 5520
ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 5580
cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 5640
gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aactgcagtt 5700
tgctagcagg accggtacat aactataaag ggctgggtca tctaagcatt tcagtcgaga 5760
aaaaaactgt ataccaaaag aacaactaac aacacttctc atctcgagac ccatatcaag 5820
atgctggatc cgggagaggt ttatgatgac cctattgatc caattgagtc agaggctgaa 5880
cccagaggaa cccccactgt ccccaacatc ttgaggaact ctgactacaa tctcaactct 5940
cctttgattg aggactctgc caaactaatg ttagaatggt tgaaaacagg gaacagacct 6000
tatcggatga ctttgacaga caattgctcc aggtcttaca aagatttgaa agattatttc 6060
aagaaagtag atttgggttc tctcaaagtg ggaggaactg ctgcacaatc aatgatttct 6120
ctctggttgt atggagccca ctctgagtca aacaggagcc ggagatgtat aaccgacttg 6180
gcccatttct attccaagtc atcccccata gagaagctgt tgaattgtac gttaggaaac 6240
agaggcctga gaatcccacc agagggggtg ttaagttgcc ttgagagggt cgattatgat 6300
aaggcatttg ggaggtatct ggccaacacg cattcctctt atctgttttt ccatgtaatt 6360
accttataca tgaatgccct agactgggaa gaggaaaaaa ccatcctagc attatggaaa 6420
gatctaacct cagtggacac cgggaaggac ttggtcaaat tcaaagatca aatatgggga 6480
ctgctgattg tgacaaagga ctttgtttac tctcagagtt ctaactgtct ttttgacaga 6540
aactatacac tgatgctaaa ggatcttttc ttgtctcgat tcaactcctt gatgattttg 6600
ctttctcccc ctgagccccg atactcagat gacttaatat ctcagctgtg ccagctatac 6660
atcgctgggg atcaagtctt gtccttgtgt gggaactccg gctatgaagt catcaaaata 6720
ttggagccat atgtcgtgaa cagtttggtc cagagggcag agaagtttag gcctctcatc 6780
cactccttgg gagactttcc tatgtttata aaagacaagg tgaatcaact tgaagggact 6840
ttcggtccca gtgcaaaaag gttttttagg gttctagatc aattcgacaa catacatgat 6900
ctagtatttg tgtatggctg ttacagacat tgggggcacc cctatataga ttatcggaag 6960
ggtctgttga aactatatga tcaagttcac attaagaaag taatagataa gtcctaccag 7020
gagtgtttag caagtgactt ggctagaagg atcctcagat ggggatttga caagtactcc 7080
aagtggtatc tagattcgag attccttgcc cgagaccacc ccttggctcc ttatatcaag 7140
acccaaacat ggccacccaa acatatagta gacttggtgg gggacacatg gcacaagctc 7200
ccgatcacgc agatctttga gattcctgaa tcaatggacc cgtcagagat actggatgat 7260
aaatcacatt ctttcaccag aacaagacta gcttcttggc tgtcagagaa ccgagggggg 7320
cctgttccta gcgagaaggt cattatcacg gccctgtctc agccacctgt caatccccga 7380
gagtttttga aatctatcga cctcggagga ttgccagatg atgacttgat aattggcctc 7440
agaccaaagg aacgggagtt gaagattgag ggccgattct tcgctctaat gtcatggaat 7500
ctaagattat attttgtcat caccgagaag ctcttggcca actacatttt gccacttttt 7560
gacgcactga ctatgacaga caacctgaac aaggtattta aaaagttgat cgacagggtc 7620
accgggcaag ggcttttgga ctattcgagg gtcacttacg catttcacct ggactatgag 7680
aaatggaaca atcatcaaag attggagtca acggaggatg tattttctgt cctagatcag 7740
gtgtttggat tgaagagggt gttttctaga acacacgagt tttttcagaa gtcctggatc 7800
tattattcag acagatcaga cctcattggg ttacgggagg atcagatata ttgcttggat 7860
atgtccaatg gtccaacctg ctggaatggc caagacggcg ggctagaggg cttacggcag 7920
aagggctgga gtctagtcag cttattgatg atagatagag aatctcaaac caggaacaca 7980
agaaccaaga tactagctca aggagacaac caggttctgt gtccgacata catgttgtca 8040
ccgggattgt ctcaagaggg gcttctctat gagttagaga gcatatcgag gaatgcactc 8100
tcaatatacc gagctatcga ggaaggagca tctaaactgg ggctgatcat caagaaagaa 8160
gagaccatgt gtagttatga ctttctcata tatgggaaga cccccttatt tcgaggcaac 8220
atattagtac ctgaatccaa aagatgggcc cgagtctctt gcatctctaa cgaccaaata 8280
gtcaacctcg ccaatataat gtctacagta tccaccaatg cgctgacagt ggcacaacac 8340
tctcaatctc tgatcaaacc tatgagggat tttctgctca tgtcagtaca ggcagttttc 8400
cactacctgc tgtttagccc aatcttaaaa ggcagggttt ataagattct gagtgctgaa 8460
ggggagagct ttctcctagc catgtcgcgg ataatctatc tagatccttc tttgggaggg 8520
gtgtctggaa tgtctctcgg gaggttccat atacgtcagt tctcagaccc tgtctctgaa 8580
gggttgtcat tctggagaga gatctggtta agctcccacg aatcctggat tcacgcgttg 8640
tgtcaagagg ccgggaaccc cgatcttgga gagagaacac tagagagctt cactcgcctt 8700
ttagaagatc ctactacctt aaatatcaaa ggaggggcca gtcctaccat tctactcaag 8760
gatgctatca gaaaggctct gtacgacgag gtggacaagg tggaaaattc agagtttcga 8820
gaggcaatcc tgttgtccaa gacccataga gataacttta tactcttttt aaaatctgtt 8880
gagcctctgt tccctcgatt tctcagtgaa ctcttcagtt cgtccttctt gggaatacca 8940
gagtcaatta ttggactgat acaaaactcc aggacaataa gaaggcagtt tagaaagagt 9000
ctctcaagaa ctttagaaga gtccttctac aactcagaga tccacgggat taatcggatg 9060
acccagacac ctcaaagggt cgggagagtg tggccctgct cttcagagag ggcagatcta 9120
cttagggaga tctcttgggg aaggaaagtg gtgggcacga cagttcctca cccttccgag 9180
atgttggggc tgcttccaaa atcctctatt tcctgcactt gtggagcaac agggggaggc 9240
aatcctagag tttctgtatc agtactcccg tccttcgatc agtcattttt ttcacgaggt 9300
cctctaaagg gatacttggg ctcgtccacc tccatgtcaa cccagctatt ccatgcatgg 9360
gaaaaagtca ctaatgttca tgtggtgaaa agggctatat cgttaaaaga atctataaac 9420
tggttcatca atagaaattc caatttggct caaactctaa ttagaaacat catgtctctg 9480
acaggccctg atttctctct agaagaggct cctgttttca aacggacagg gtcagccttg 9540
cataggttca agtctgccag atacagcgaa ggagggtatt cttctgtttg tcctaacctt 9600
ctctctcata tctctgtcag tacagacact atgtctgatt tgacccaaga cgggaagaac 9660
tatgatttca tgtttcagcc attgatgctt tatgcgcaaa catggacatc agagctggta 9720
cagagagaca caagactgag agactccacg tttcactggc accttcgatg caacagatgt 9780
gtaaggccca ttgaggatat aacactggaa acttctcaga tcttcgagtt cccggatgtg 9840
tcaaaaagga tatccaggat ggtttctgga gccgtccctc actttcagaa gcttcctgat 9900
atccgtctaa gaccaggtga ttttgaatct ctaagtggta gagaaaagtc tcgccacata 9960
gggtcagctc aggggctctt atactcaatc ttagtagcaa ttcacgattc aggatacaat 10020
gatgggacca tcttccctgt caacatatac ggcaaggttt cccctagaga ctatttgaga 10080
gggctcgcaa gagggatctt gataggatcc tcgatttgct tcttgacacg aatgacaaat 10140
attaatatta aaagacctct tgaattgatc tcaggggtaa tttcctatat tctcctgagg 10200
ctggataatc atccctctct gtatataatg cttagagaac cgtctcttag aggagagata 10260
ttctctatcc ctcagaagat ccccgccgct tacccaacca ctatgaaaga aggcaacaga 10320
tcgatcttgt gttacctcca acacgtgcta cgctatgagc gagaagtaat cacggcgtcc 10380
ccggagaatg actggctgtg gatcttctca gacttcagaa gtgcgaaaat gacgtacttg 10440
accctcatta cctaccagtc tcacctccta ctccagaggg ttgagcgaaa cttgtctaag 10500
agtatgagag ctactctgca acaaatgggt tccttaatga ggcaagtgct gggtgggcac 10560
ggagaagaca ccttggagtc agacgacgac attcaacgat tactaaaaga ctctttgcga 10620
aggacgaggt gggtagatca agaggtgcgc catgcagcta gaaccatgag tggagattac 10680
agccccaaca agcttaagag ccatactctt tatgcccccg gtggatatga cattatggga 10740
tatctgaggc agatcaggaa ccggccaaac ccgcaggtgg aactgggccc cgtggataca 10800
tcctgcgcct tgattctttg tgacctgaaa cagaaagaca ccccgatagt ttacgcgagt 10860
gaagccttcc tctacatgac aggttacagc aacgcagagg tgctgggccg gaattgccgg 10920
tttctgcaaa gccctgacgg catggtgaag cccaagagca cccggaagta cgtggatagt 10980
aacacaatca atactatgcg caaggcaatc gacaggaatg ccgaggtgca ggttgaagta 11040
gtcaatttta aaaagaatgg acagcgattt gttaatttcc tgactatgat acctgttagg 11100
gacgaaacag gcgagtatcg atactctatg ggattccagt gcgaaacaga aaccggtaat 11160
tccgctgacg gcggcggagg atcgggtggt agtggtggtt caggaggagg atcgacccaa 11220
ggaggatcca tgcatactct ttatgccccc ggtggatatg acattatggg atatctggac 11280
cagatcggca accggccaaa cccgcaggtg gaactgggcc ccgtggatac atcctgcgcc 11340
ttgattcttt gtgacctgaa acagaaagac accccgatag tttacgcgag tgaagccttc 11400
ctctacatga caggttacag caacgcagag gtgctgggcc ggaattgccg gtttctgcaa 11460
agccctgacg gcatggtgaa gcccaagagc acccggaagt acgtggatag taacacaatc 11520
aatactatcc gcaaggcaat cgacaggaat gccgaggtgc aggttgaagt agtcaatttt 11580
aaaaagaatg gacagcgatt tgttaatttc ctgactatca tacctgttag ggacgaaaca 11640
ggcgagtatc gatactctat gggattccag tgcgaaacag aatatccgta cgacgtccca 11700
gattacgcgg gccttaaggt atctcgcaag gcaggatgtt cagaatgggt ctgctctgct 11760
caacaggttg ccgtctccac ctcagccaac ccggcccctg tctcagagct tgacattagg 11820
gccctatcta agaggtttca aaaccccttg atctcgggcc taagagtggt tcagtgggca 11880
accggcgccc attataagct aaagcctatt ctagatgatc taaatgtttt cccatctctc 11940
tgtcttgttg ttggagacgg gtcaggggga atatcaaggg cagttctcaa catgtttcca 12000
gattctaagc ttgtgttcaa cagcctattg gaggtgaatg atctgatggc ttccggaaca 12060
cgtccactgc ctccttcagc aatcatgagt ggaggagatg atatcatctc cagagtgata 12120
gactttgact caatctggga aaaaccatcc gacctgagga acttggccac ctggagatac 12180
ttccagtcag ttcaaaagca ggtcaacatg tcgtatgacc tcattatttg tgatgcagaa 12240
gttactgata ttgcatctat caaccggata actctgttga tgtctgattt cgcattgtct 12300
atagatggac cactttatct ggtcttcaaa acttacggga ctatgctagt gaacccagac 12360
tataaagcta tccaacatct gtcaagagcg ttcccttcgg tcacagggtt tataacccaa 12420
gtaacttcgt ccttttcttc tgagctgtac ctccggttct ccaaacgagg gaagtttttt 12480
agggatgctg agtacttgac ctcttccacc cttcgggaga tgagccttgt gttattcaat 12540
tgtagcagcc ccaaaagtga gatgcagaga gctcgttcct taaactatca agatcttgta 12600
aggggatttc ctgaagagat catatcaaat ccttacaatg aaatgatcat aactctgatt 12660
gacagtgatg tagagtcctt cctggtccac aagatggtgg atgatcttga gttacagagg 12720
ggaactctgt ctaaagtggc tatcattata tccatcatga tcgttttttc caacagagtc 12780
ttcaacattt ccaaaccttt gactgacccc ttgttctatc ccccatctga tcctaaaatc 12840
ctgaggcact tcaacatatg ttgcagtact atgatgtatc tatctaccgc tttaggcgac 12900
gtccctagct tcgcaagact tcatgaccta tataatagac ctataactta ttacttcaga 12960
aagcaagtta ttcgagggaa tatctatcta tcttggagct ggtccgatga caccccagtg 13020
ttcaagaggg tagcctgtaa ttctagcttg agtctgtcat ctcactggat caggttgatt 13080
tacaagatag tgaagactac cagactcgtt ggccgcatag aagatctatc aggagaggta 13140
gaacgacacc tgcatgggta caacagatgg atcaccctcg aggatatccg atctagatca 13200
tccctactag actacagttg tttgtaagcc ggacattaat gaaagcctgt acatgctaaa 13260
attcttgtat gatgcatctt gaaaaaaaca agatcttgaa tcagaacctc tggttgtttg 13320
attgtttttt ttatctttat tgtttatttg ttaagcgtgg gtcggcatgg catctccacc 13380
tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga tggctaaggg 13440
agggcgggta ccacgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 13500
ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 13560
gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac 13620
gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 13680
acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 13740
ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 13800
gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 13860
tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 13920
ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 13980
gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 14040
gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc 14100
gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 14160
aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 14220
tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 14280
aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 14340
aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 14400
tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 14460
aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 14520
tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 14580
ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 14640
taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 14700
agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 14760
caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 14820
tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 14880
gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 14940
cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 15000
caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 15060
ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 15120
aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 15180
gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 15240
atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 15300
tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 15360
gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 15420
actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 15480
gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 15540
agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 15600
ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 15660
aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 15720
cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 15780
gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 15840
cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 15900
cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 15960
gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 16020
aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 16080
actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 16140
cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 16200
aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgctgta cgggccagat 16260
atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 16320
ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 16380
gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 16440
caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 16500
cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 16560
ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 16620
tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 16680
gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 16740
gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 16800
tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 16860
taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 16920
cccaagctgg ctagtgttaa gcgtctgatg agtccgtgag gacgaaacta taggaaagga 16980
attcctatag tc 16992
<210> 54
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> 正向1引物
<400> 54
tgttacctcc aacacgtgct acg 23
<210> 55
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> 正向2引物
<400> 55
caacaagctt aagagccata ctctttatgc ccccg 35
<210> 56
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> 正向3引物
<400> 56
cgggccttaa ggtatctcgc aaggcaggat 30
<210> 57
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> 反向1引物
<400> 57
ctcttaagct tgttggggct gtaatctc 28
<210> 58
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> 反向2引物
<400> 58
ataccttaag gcccgcgtaa tctgggac 28
<210> 59
<211> 17
<212> DNA
<213> Artificial Sequence
<220>
<223> 反向3引物
<400> 59
atgcccaggt cggaccg 17
<210> 60
<211> 6355
<212> DNA
<213> Artificial Sequence
<220>
<223> pH-N
<400> 60
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900
acaatggatg ccgacaagat tgtgttcaaa gtcaataacc gggtggtctc tttgaagccc 960
gagattatcg tggatcaata tgagtacaag taccctgcta tcaaagattt gaaaaagcct 1020
tgtataaccc tagggaaagc ccccgattta aacaaagctt acaaatcagt tttatcaggc 1080
atgaatgccg ccaaacttga tcctgatgat gtatgctcct acttggcagc agcaatgcag 1140
ttctttgagg ggacatgtcc ggaagactgg accagctatg gaatcctgat tgcacgaaaa 1200
ggagacaaga tcaccccaga ctctctagtg gagataaagc gtactgatgt agaagggaat 1260
tgggctctga caggaggcat ggaactgaca agggacccca ctgtctctga acatgcatct 1320
ttagtcggtc ttctcctgag tctgtacaga ttgagcaaaa tatcaggaca aaacactggt 1380
aactataaga caaacattgc ggatagaata gagcagattt tcgagacagc cccttttgtt 1440
aagatcgtgg aacaccacac cctgatgaca actcacaaga tgtgtgctaa ttggagtact 1500
ataccgaact tcagattttt ggccggaacc tacgacatgt ttttctcacg gatcgagcat 1560
ctgtattcgg caatcagagt gggcacagtt gtcaccgctt atgaagactg ctcaggactg 1620
gtatcgttta cagggttcat aaagcagatc aatctcaccg caagagaagc aatactatat 1680
ttcttccaca agaactttga agaagagata aggagaatgt ttgagccagg gcaagagaca 1740
gctgttcctc actcttattt cattcacttc cgttcactag gcttgagtgg gaagtctcct 1800
tattcatcaa atgccgtcgg tcatgtgttc aatctcattc actttgttgg atgctatatg 1860
ggtcaagtca gatctctaaa tgccacggtt attgctgcat gtgcccctca tgagatgtct 1920
gttctagggg gctatttggg agaggagttc ttcggaaaag ggacatttga aagaaggttc 1980
ttcagagacg agaaagaact tcaagaatat gaggcggctg aactgacaaa gaccgacgtg 2040
gcactggcag atgacggaac tgtcaactcc gatgacgagg actacttctc cggtgaaacc 2100
agaagtcctg aagctgtcta tactcgaatc atgatgaatg gaggtcgact gaagagatca 2160
catatacgga gatatgtctc agtcagttcc aatcatcaag cccgtccaaa ctcattcgcc 2220
gaatttttaa acaaaacgta ttcgagtgac tcataaggta ccgagctcgg atccactagt 2280
ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc 2340
ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 2400
gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 2460
aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 2520
tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 2580
tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 2640
cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 2700
cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 2760
tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 2820
ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 2880
cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 2940
tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 3000
ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 3060
cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg 3120
caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3180
caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3240
tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3300
cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 3360
tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 3420
gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata 3480
gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag 3540
tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg 3600
gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt 3660
gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt 3720
gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt 3780
ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt 3840
cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca 3900
cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 3960
tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 4020
ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 4080
tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 4140
tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 4200
atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 4260
aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 4320
gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 4380
ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 4440
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 4500
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 4560
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 4620
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 4680
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 4740
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 4800
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 4860
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 4920
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 4980
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 5040
gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 5100
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 5160
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 5220
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 5280
cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 5340
gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 5400
tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 5460
gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 5520
agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 5580
tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 5640
agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 5700
gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 5760
catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 5820
ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 5880
atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 5940
tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 6000
cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 6060
cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 6120
atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 6180
aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 6240
ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 6300
aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc 6355
<210> 61
<211> 5899
<212> DNA
<213> Artificial Sequence
<220>
<223> pH-P
<400> 61
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900
gtttaaactt aagcttggta ccatcccaag tatgagcaag atctttgtta atccgagtgc 960
aatcagagcc ggtctggccg atcttgagat ggccgaagag actgttgatc tgatcaacag 1020
aaacatagaa gacaatcagg ctcatctcca gggagaaccc atagaagtgg acaatctccc 1080
tgaggacatg aggcaatttc acctggacga tgaaaaattg tccaaccttg gtgagatggt 1140
tagggtgggc gaaggcaagt atcgagagga ctttcagatg aatgagggag aggaccccaa 1200
cctcctgttc caatcgtacc tggacaatgt tggagtccaa atagtcagac aaatgaggtc 1260
aggagagaga ttcctcaaga tatggtcaca gaccgtagag gaaattatat cctatgtctc 1320
ggtcaacttt cctaaccctc caggaaggtc ttcggaggat aaatcaaccc aaactactgg 1380
ccgggagctc aagaaggaga caacatccat tctttctcaa agagaaagcc aaccttcaaa 1440
agccggaatg gtggctcaag ttgcctctgg ccctccatcc cttgaatggt ctgccaccaa 1500
tggagaggat gatctatcag tagaggctga gatcgctcat cagattgctg aaagcttttc 1560
caagaagtac aagtttcctt ctcgatcttc aggaatattc ttgtataatt ttgagcaact 1620
ggagatgaac cttgatgaca tagttaaaga ggcaaaaaat gtaccgggcg tgacccgtct 1680
ggcccatgat ggatccaaaa tccccctaag atgtgtactg ggatgggtcg ctttggccaa 1740
ctccaagaaa ttccaattgc tagtcgaggc cgacaagcta agcaaaatca tgcaagatga 1800
cttggatcgc tacacatcat gctaaccgag tcttcgaatt ctgcagatat ccagcacagt 1860
ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt 1920
ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 1980
ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 2040
gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 2100
atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 2160
ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 2220
tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 2280
tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca 2340
tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 2400
gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 2460
agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 2520
cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg 2580
agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 2640
tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt 2700
cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2760
atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2820
cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2880
ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2940
taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg 3000
tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa 3060
ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg 3120
agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt 3180
cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt 3240
gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg 3300
gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg 3360
cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt 3420
cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta 3480
tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 3540
ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 3600
caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 3660
ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 3720
ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 3780
aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 3840
gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 3900
gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 3960
ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4020
atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 4080
gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 4140
gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 4200
gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 4260
gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4320
aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 4380
ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 4440
taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 4500
tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 4560
gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 4620
taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 4680
tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 4740
tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4800
ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 4860
taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 4920
tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 4980
cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 5040
gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 5100
cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 5160
ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 5220
aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 5280
atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 5340
tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 5400
gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 5460
aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 5520
acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 5580
ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 5640
tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 5700
aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 5760
catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 5820
atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 5880
aaaagtgcca cctgacgtc 5899
<210> 62
<211> 11373
<212> DNA
<213> Artificial Sequence
<220>
<223> pH-L
<400> 62
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900
aagatgctgg atccgggaga ggtttatgat gaccctattg atccaattga gtcagaggct 960
gaacccagag gaacccccac tgtccccaac atcttgagga actctgacta caatctcaac 1020
tctcctttga ttgaggactc tgccaaacta atgttagaat ggttgaaaac agggaacaga 1080
ccttatcgga tgactttgac agacaattgc tccaggtctt acaaagattt gaaagattat 1140
ttcaagaaag tagatttggg ttctctcaaa gtgggaggaa ctgctgcaca atcaatgatt 1200
tctctctggt tgtatggagc ccactctgag tcaaacagga gccggagatg tataaccgac 1260
ttggcccatt tctattccaa gtcatccccc atagagaagc tgttgaattg tacgttagga 1320
aacagaggcc tgagaatccc accagagggg gtgttaagtt gccttgagag ggtcgattat 1380
gataaggcat ttgggaggta tctggccaac acgtattcct cttatctgtt tttccatgta 1440
attaccttat acatgaatgc cctagactgg gaagaggaaa aaaccatcct agcattatgg 1500
aaagatctaa cctcagtgga caccgggaag gacttggtca aattcaaaga tcaaatatgg 1560
ggactgctga ttgtgacaaa ggactttgtt tactctcaga gttctaactg tctttttgac 1620
agaaactata cactgatgct aaaggatctt ttcttgtctc gattcaactc cttgatgatt 1680
ttgctttctc cccctgagcc ccgatactca gatgacttaa tatctcagct gtgccagcta 1740
tacatcgctg gggatcaagt cttgtccttg tgtgggaact ccggctatga agtcatcaaa 1800
atattggagc catatgtcgt gaacagtttg gtccagaggg cagagaagtt taggcctctc 1860
atccactcct tgggagactt tcctatgttt ataaaagaca aggtgaatca acttgaaggg 1920
actttcggtc ccagtgcaaa aaggtttttt agggttctag atcaattcga caacatacat 1980
gatctagtat ttgtgtatgg ctgttacaga cattgggggc acccctatat agattatcgg 2040
aagggtctgt tgaaactata tgatcaagtt cacattaaga aagtaataga taagtcctac 2100
caggagtgtt tagcaagtga cttggctaga aggatcctca gatggggatt tgacaagtac 2160
tccaagtggt atctagattc gagattcctt gcccgagacc accccttggc tccttatatc 2220
aagacccaaa catggccacc caaacatata gtagacttgg tgggggacac atggcacaag 2280
ctcccgatca cgcagatctt tgagattcct gaatcaatgg acccgtcaga gatactggat 2340
gataaatcac attctttcac cagaacaaga ctagcttctt ggctgtcaga gaaccgaggg 2400
gggcctgttc ctagcgagaa ggtcattatc acggccctgt ctcagccacc tgtcaatccc 2460
cgagagtttt tgaaatctat cgacctcgga ggattgccag atgatgactt gataattggc 2520
ctcagaccaa aggaacggga gttgaagatt gagggccgat tcttcgctct aatgtcatgg 2580
aatctaagat tatattttgt catcaccgag aagctcttgg ccaactacat tttgccactt 2640
tttgacgcac tgactatgac agacaacctg aacaaggtat ttaaaaagtt gatcgacagg 2700
gtcaccgggc aagggctttt ggactattcg agggtcactt acgcatttca cctggactat 2760
gagaaatgga acaatcatca aagattggag tcaacggagg atgtattttc tgtcctagat 2820
caggtgtttg gattgaagag ggtgttttct agaacacacg agttttttca gaagtcctgg 2880
atctattatt cagacagatc agacctcatt gggttacggg aggatcagat atattgcttg 2940
gatatgtcca atggtccaac ctgctggaat ggccaagacg gcgggctaga gggcttacgg 3000
cagaagggct ggagtctagt cagcttattg atgatagata gagaatctca aaccaggaac 3060
acaagaacca agatactagc tcaaggagac aaccaggttc tgtgtccgac atacatgttg 3120
tcaccgggat tgtctcaaga ggggcttctc tatgagttag agagcatatc gaggaatgca 3180
ctctcaatat accgagctat cgaggaagga gcatctaaac tggggctgat catcaagaaa 3240
gaagagacca tgtgtagtta tgactttctc atatatggga agaccccctt atttcgaggc 3300
aacatattag tacctgaatc caaaagatgg gcccgagtct cttgcatctc taacgaccaa 3360
atagtcaacc tcgccaatat aatgtcgaca gtgtccacca atgcgctgac agtggcacaa 3420
cactctcaat ctctgatcaa acctatgagg gattttctgc tcatgtcagt acaggcagtt 3480
ttccactacc tgctgtttag cccaatctta aaaggcaggg tttacaagat tctgagtgct 3540
gaaggggaga gctttctcct agccatgtcg cggataatct atctagatcc ttctttggga 3600
ggggtgtctg gaatgtctct cgggaggttc catatacgtc agttctcaga ccctgtctct 3660
gaagggttgt cattctggag agagatctgg ttaagctccc acgaatcctg gattcacgcg 3720
ttgtgtcaag aggccgggaa ccccgatctt ggagagagaa cactagagag cttcactcgc 3780
cttttagaag atcctactac cttaaatatc aaaggagggg ctagtcctac cattctactc 3840
aaggatgcta tcagaaaggc tctgtacgac gaggtggaca aggtggaaaa ttcagagttt 3900
cgagaggcaa tcctgttgtc caagacccat agagataact ttatactctt tttaaaatct 3960
gttgagcctc tgttccctcg atttctcagt gaactcttca gttcgtcctt cttgggaata 4020
ccagagtcaa ttattggact gatacaaaac tccaggacaa taagaaggca gtttagaaag 4080
agtctctcaa gaactttaga agagtccttc tacaactcag agatccacgg gattaatcgg 4140
atgacccaga cacctcaaag ggtcgggaga gtgtggccct gctcttcaga gagggcagat 4200
ctacttaggg agatctcttg gggaaggaaa gtggtgggca cgacagttcc tcacccttcc 4260
gagatgttgg ggctgcttcc aaaatcctct atttcctgca cttgtggagc aacaggggga 4320
ggcaatccta gagtttctgt atcagtactc ccgtccttcg atcagtcatt tttttcacga 4380
ggtcctctaa agggatactt gggctcgtcc acctccatgt caacccagct attccatgca 4440
tgggaaaaag tcactaatgt tcatgtggtg aaaagggcta tatcgttaaa agaatctata 4500
aactggttca tcaatagaaa ttccaatttg gctcaaactc taattagaaa catcatgtct 4560
ctgacaggcc ctgatttctc tctagaagag gctcctgttt tcaaacggac agggtcagcc 4620
ttgcataggt tcaagtctgc cagatacagc gaaggagggt attcttctgt ttgtcctaac 4680
cttctctctc atatctctgt cagtacagac actatgtctg atttgaccca agacgggaag 4740
aactatgatt tcatgtttca gccattgatg ctttatgcgc aaacatggac atcagagctg 4800
gtacagagag acacaagact gagagactcc acgtttcact ggcaccttcg atgcaacaga 4860
tgtgtaaggc ccattgagga tataacactg gaaacttctc agatcttcga gttcccggat 4920
gtgtcaaaaa ggatatccag gatggtttct ggagccgtcc ctcactttca gaagcttcct 4980
gatatccgtc taagaccagg tgattttgaa tctctaagtg gtagagaaaa gtctcgccac 5040
atagggtcag ctcaggggct cttatactca atcttagtag caattcacga ttcaggatac 5100
aatgatggga ccatcttccc tgtcaacata tacggcaagg tttcccctag agactatttg 5160
agagggctcg caagagggat cttgatagga tcctcgattt gcttcttgac acgaatgaca 5220
aatattaata ttaaaagacc tcttgaattg atctcagggg taatttccta tattctcctg 5280
aggctggata atcatccctc tctgtatata atgcttagag aaccgtctct tagaggagag 5340
atattctcta tccctcagaa gatccccgcc gcttacccaa ccactatgaa agaaggcaac 5400
agatcgatct tgtgttacct ccaacacgtg ctacgctatg agcgagaagt aatcacggcg 5460
tccccggaga atgactggct gtggatcttc tcagacttca gaagtgcgaa aatgacgtac 5520
ttgaccctca ttacctacca gtctcacctc ctactccaga gggttgagcg aaacttgtct 5580
aagagtatga gagctactct gcaacaaatg ggttccttaa tgaggcaagt gctgggtggg 5640
cacggagaag acaccttgga gtcagacgac gacattcaac gattactaaa agactctttg 5700
cgaaggacga ggtgggtaga tcaagaggtg cgccatgcag ctagaaccat gagtggagat 5760
tacagcccca acaagagagt atctcgcaag gcaggatgtt cagaatgggt ctgctctgct 5820
caacaggttg ccgtctccac ctcagccaac ccggcccctg tctcagagct tgacattagg 5880
gccctatcta agaggtttca aaaccccttg atctcgggcc taagagtggt tcagtgggca 5940
accggcgccc attataagct taagcctatt ctagatgatc taaatgtttt cccatctctc 6000
tgtcttgttg ttggagacgg gtcaggggga atatcaaggg cagttctcaa catgtttcca 6060
gattctaagc ttgtgttcaa cagcctattg gaggtgaatg atctgatggc ttccggaaca 6120
cgtccactgc ctccttcagc aatcatgagt ggaggagatg atatcatctc cagagtgata 6180
gactttgact caatctggga aaaaccatcc gacctgagga acttggccac ctggagatac 6240
ttccagtcag ttcaaaagca ggtcaacatg tcgtatgacc tcattatttg tgatgcagaa 6300
gttactgata ttgcatctat caaccggata actctgttga tgtctgattt cgcattgtct 6360
atagatggac cactttatct ggtcttcaaa acttacggga ctatgctagt gaacccagac 6420
tataaagcta tccaacatct gtcaagagcg ttcccttcgg tcacagggtt tataacccaa 6480
gtaacttcgt ccttttcttc tgagctgtac ctccggttct ccaaacgagg gaagtttttt 6540
agggatgctg agtacttgac ctcttccacc cttcgggaga tgagccttgt gttattcaat 6600
tgtagcagcc ccaaaagtga gatgcagaga gctcgttcct taaactatca agatcttgta 6660
aggggatttc ctgaagagat catatcaaat ccttacaatg aaatgatcat aactctgatt 6720
gacagtgatg tagagtcctt cctggtccac aagatggtgg atgatcttga gttacagagg 6780
ggaactctgt ctaaagtggc tatcattata tccatcatga tcgttttttc caacagagtc 6840
ttcaacattt ccaaaccttt gactgacccc ttgttctatc ccccatctga tcctaaaatc 6900
ctgaggcact tcaacatatg ttgcagtact atgatgtatc tatctaccgc tttaggcgac 6960
gtccctagct tcgcaagact tcatgaccta tataatagac ctataactta ttacttcaga 7020
aagcaagtta ttcgagggaa tatctatcta tcttggagct ggtccgatga caccccagtg 7080
ttcaagaggg tagcctgtaa ttctagcttg agtctgtcat ctcactggat caggttgatt 7140
tacaagatag tgaagactac cagactcgtt ggccgcatag aagatctatc aggagaggta 7200
gaacgacacc tgcatgggta caacagatgg atcaccctcg aggatatccg atctagatca 7260
tccctactag actacagttg tttgtaagcc ggacattaat gaaaagggcg aattctgcag 7320
atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc 7380
ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 7440
gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 7500
ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 7560
ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 7620
ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 7680
cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 7740
cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 7800
tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 7860
aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 7920
ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 7980
actcaaccct atctcggtct attcttttga tttataaggg attttgggga tttcggccta 8040
ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 8100
tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat 8160
gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 8220
tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 8280
cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 8340
tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 8400
cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 8460
atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac 8520
gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg 8580
cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc 8640
gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc 8700
caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag 8760
ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc 8820
atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc 8880
aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc 8940
accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 9000
atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 9060
gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 9120
tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 9180
ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 9240
tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 9300
ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 9360
tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 9420
ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 9480
ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 9540
gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 9600
gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 9660
cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 9720
ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 9780
tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 9840
gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 9900
tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 9960
ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 10020
ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 10080
ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 10140
accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 10200
tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 10260
cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 10320
taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 10380
caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 10440
gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 10500
gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 10560
ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 10620
attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 10680
gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 10740
tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 10800
agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 10860
gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 10920
actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 10980
tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 11040
attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 11100
tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 11160
tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 11220
aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 11280
tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 11340
cgcacatttc cccgaaaagt gccacctgac gtc 11373
<210> 63
<211> 6648
<212> DNA
<213> Artificial Sequence
<220>
<223> pH-G
<400> 63
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900
gtttaaactt aaggaaagat ggttcctcag gttcttttgt ttgcacccct cctggtttct 960
ccattgtgtt tcgggaagtt ccccatttac acgataccag acaaacttgg tccctggagc 1020
cctattgact tacaccatct cagctgtcca aataacctgg ttgtggagga cgaaggatgt 1080
accaacctgt ccgggttctc ttacatggaa cttaaagtgg gatacatctc agccataaaa 1140
gtgaacgggt tcacttgcac aggtgttgtg acagaggcag aaacctacac caactttgtt 1200
ggttatgtca caaccacatt caagagaaag catttccgcc ccaccccaga cgcatgtaga 1260
gccgcgtata actggaagat ggccggtgac cccagatatg aagagtctct acacaatccg 1320
taccccgact accattggct tcgaactgta aaaaccacca aagagtctct cgttatcata 1380
tccccaagtg tgacagattt ggacccatat gacaaatccc ttcactcaag ggtcttccct 1440
ggcggaaatt gctcaggaat aacggtgtcc tcgacctact gctcaactaa tcatgattac 1500
accatctgga tgcctgagaa tctgagacta gggacatctt gtgacatttt tacccatagc 1560
agagggaaga gagcatccaa aggagacaag acttgcggct ttgtggatga aagaggcctg 1620
tataagtctt taaagggagc atgcaaactc aagttatgtg gagttctcgg acttagactt 1680
atggatggaa catgggtcgc gatgcaaaca tcagatgaga ccaaatggtg ccctccaggt 1740
cagttggtga atttgcacga ctttcgctca gacgagattg agcatctcgt tgaggaagag 1800
ttagtcaaga aaagagagga gtgtctggat gcactagagt ccatcatgac caccaagtca 1860
gtgagtttca gacgtctcag tcacctgaga aaacttgtcc ctgggtttgg aaaagcatat 1920
accatattca acaaaacctt gatggaggct gatgctcact acaagtctgt ccagacctgg 1980
aatgagatca tcccctcaaa agggtgtttg agagttgggg agaggtgtca tccccatgtg 2040
aacggggtgt ttttcaatgg tataatatta gggtctgacg gccatgttct aatcccagag 2100
atgcagtcat ccctcctcca gcaacatatg gagttgttgg aatcttcagt tatccccctg 2160
atgcacccct tggcagaccc ttctacagtt ttcaaagacg gtgatgaggt tgaggatttt 2220
gttgaagttc acctccccga tgtgcataaa caggtctcag gagttgacct gggtctcccg 2280
aaatggggga agtatgtatt gatgattgca ggggccttga ttgccctgat gttgataatt 2340
ttcctgatga catgttgcag aagagtcaat cgaccagaat ctacacaaag caatcttgga 2400
gggacaggga gaaatgtgtc agtcccttcc caaagcggaa aagtcatatc ttcatgggag 2460
tcatataaga gtggaggcga gaccagactg tgaaggccgg tcatcctttt gacacctcaa 2520
gtccagagga taacctcctc tcggggttgg ggggaatctt gggatccact agtccagtgt 2580
ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta 2640
aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 2700
ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 2760
ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 2820
ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 2880
tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 2940
tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 3000
cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 3060
ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg 3120
gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 3180
atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 3240
ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 3300
ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 3360
attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag 3420
aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3480
cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3540
cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3600
ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 3660
gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 3720
tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc 3780
ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt 3840
ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg 3900
ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg 3960
ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg 4020
cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac 4080
gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg 4140
cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta 4200
cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 4260
gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 4320
aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 4380
aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 4440
tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 4500
tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 4560
aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 4620
ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 4680
gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 4740
cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4800
tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4860
aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 4920
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 4980
caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 5040
ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt 5100
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 5160
gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 5220
cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 5280
ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 5340
tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 5400
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 5460
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 5520
tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 5580
tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 5640
tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 5700
cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 5760
ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 5820
tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 5880
gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 5940
agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 6000
atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 6060
tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 6120
gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 6180
agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 6240
cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 6300
ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 6360
ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 6420
actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 6480
ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 6540
atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 6600
caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc 6648
<210> 64
<211> 13208
<212> DNA
<213> Artificial Sequence
<220>
<223> pHS 717-AG1 r1
<400> 64
actagtattt aggtgacact atagcaatgg gagctaacgg acctcgctta ggactcctat 60
tcccatggag aaactcctag atgaggttct tgcccccggt gggccttata acttaaccgt 120
cggcagttgg gtaagagacc atgtccgctc aattgtcgag ggcgcgtggg aagtgcgcga 180
tgtcgtcacc gctgcccaaa agcgggccat cgtagctgtg atacccagac ctgtattcac 240
gcagatgcag gttagtgatc acccagcact ccacgcaatt tcgcggtata cccgccgcca 300
ttggatcgag tggggcccta aagaagccct acacgtcctc atcgacccga gcccgggcct 360
gctccgcgag gtcgctcgcg ttgagcgccg ctgggtcgca ctgtgcctcc acaggacggc 420
acgcaaactc gccaccgccc tggccgagac ggccggcgag gcgtggcatg ccgactacgt 480
gtgcgcgctg cgtggcgcac cgagcggccc cttctacgtc caccctgagg acgtcccgca 540
cggcggccgc gccgtggcgg acagatgctt gctctactac acacccatgc agatgtgcga 600
gctgatgcgt gccattgacg ccaccttgct cgtggcggtt gacttgtggc cggtcgccct 660
tgcggctcat gtcggcgacg actgggacga cctgggcatc gcctggcacc tcgaccatga 720
cggcggctgc cccgccgact gccgcggagc cggcgctggg cccacgcccg gctacacccg 780
cccctgcacc acacgcatct accaagtcct gccggacacc gcccaccccg ggcgcctcta 840
ccggtgcggg ccccgcctgt ggacgcgcga ttgcgccgtg gccgaactct catgggaggt 900
tgcccagcac tgcgggcacc aggcgcgcgt gcgcgccgtg cgatgcaccc tccccatccg 960
ccacgtgcgc agcctccaac ccagcgcacg ggtccgactc ccggacctcg tccatctcgc 1020
cgaggtgggc cggtggcggt ggttcagcct cccccgcccc gtgttccagc gcatgctgtc 1080
ctactgcaag accctgagcc ccgacgcgta ctacagcgag cgtgtgttca agttcaagaa 1140
cgccctgagc cacagcatca cgctcgcggg caatgtgctg caagaggggt ggaagggcac 1200
gtgcgccgag gaagacgcgc tgtgcgcata cgtagccttc cgcgcgtggc agtctaacgc 1260
caggttggcg gggatcatga aaggcgctaa gcgctgcgct gccgactctt tgagcgtggc 1320
cggctggctg gacaccattt gggacgccat caagcggttc ttcggcagcg tgcccctcgc 1380
cgagcgcatg gaggagtggg aacaggacgc cgcggtcgcc gccttcgacc gcggccccct 1440
tgaggacggc gggcgccact tggacaccgt gcaacccccg aagtcgccgc cccgccctga 1500
gatcgccgcg acctggatcg tccacgcagc cagcgcagac cgccattgcg cgtgcgctcc 1560
ccgctgcgac gtcccgcgcg aacgtccttc cgcgcccgcc ggcccgcccg atgacgaggc 1620
gctcatcccg ccgtggctgt tcgccgagcg ccgtgccctc cgctgccgcg agtgggactt 1680
tgaggctctc cgcgcgcgcg ccgatacggc ggccgcgccc gccccgctgg ccccacgccc 1740
tgcgcggtac cccactgtgc tctaccgcca ccccgcccac cacggcccgt ggctcaccct 1800
tgacgagccg ggcgaggctg acgcggccct ggtcttatgc gacccgcttg gccagccgct 1860
ccggggccct gaacgccact tcgccgccgg cgcgcacatg tgcgcgcagg cgcgcgggct 1920
ccaggctttt gtccgtgttg tgcctccacc cgaacgcccc tgggccgacg ggggcgccag 1980
agcgtgggca aaattcttcc gcggctgcgc ctgggcgcag cgcttgctcg gcgagccggc 2040
agtgatgcac ctcccataca ccgatggtga cgtgccacag ctgatcgcgc tggccttgcg 2100
cacgctggcc cagcaggggg ccgccttggc actctcggtg cgagacctgc ccgggggtgc 2160
agcgttcgac gcgaatgcgg ttaccgccgc agtgcgcgct ggccccggcc agtccaagct 2220
tggtggttca ggaggaggat cgacccaagg aaccggtatg gtgagcgtga tcaagcccga 2280
gatgaagatc aagctgtgca tgaggggcac cgtgaacggc cacaacttcg tgatcgaggg 2340
cgagggcaag ggcaacccct acgagggcac ccagatcctg gacctgaacg tgaccgaggg 2400
cgcccccctg cccttcgcct acgacatcct gaccaccgtg ttccagtacg gcaacagggc 2460
cttcaccaag taccccgccg acatccagga ctacttcaag cagaccttcc ccgagggcta 2520
ccactgggag aggagcatga cctacgagga ccagggcatc tgcaccgcca ccagcaacat 2580
cagcatgagg ggcgactgct tcttctacga catcaggttc gacggcacca acttcccccc 2640
caacggcccc gtgatgcaga agaagaccct gaagtgggag cccagcaccg agaagatgta 2700
cgtggaggac ggcgtgctga agggcgacgt gaacatgagg ctgctgctgg agggcggcgg 2760
ccactacagg tgcgacttca agaccaccta caaggccaag aaggaggtga ggctgcccga 2820
cgcccacaag atcgaccaca ggatcgagat cctgaagcac gacaaggact acaacaaggt 2880
gaagctgtac gagaacgccg tggccaggta ctccatgctg cccagccagg ccaagaccgg 2940
taattccgct gacggcggcg gaggatcggg tggtagtaag cttgcggcca cgtcaccgcc 3000
acccggcgac cccccgccac cgcgccgcgc acggcgatcg caacggcact cggacgcccg 3060
cagcactccg ccctccgcgc ctgcgcgcga cccgccgccg cccgccccca gcccgcccgc 3120
gccaccccgc gcgggtgacc tggccccccc cattctcgcg gggccggcgg atcgcgcgcg 3180
tgacgccgag ctggaggtcg cctgcgaacc gagcggcccc cccgcgccaa ccaaggcaga 3240
cccagacagc gacatcgttg aaagttacgc ccgcgccgcc gggcccgtgc acctccgggt 3300
ccgtgacatc atggacccac cgcctgggtg caaggtcgtg gtcaacgccg ccaacgaggg 3360
gctgctggcc ggctttggcg tgtgtggtgc catctttgcc aacgccacgg cggcccttgc 3420
tgcagactgc cggcgcctcg ccccatgccc caccggcgag gcagtggcga cacccggcca 3480
cggctgcggg tacacccaca tcatccacgc cgttgccccg cggcgccctc gggaccccgc 3540
cgccctcgag gagggcgagg cactgctcga gcgcgcctac cgcagcatcg tcgcgctagc 3600
tgctgcgcgt aggtggacgt gtatcgcgtg ccccctcctc ggcgctggcg tctacggctg 3660
gtctgctgcg gagtccctcc gcgccgcgct tgcggccacg cgcgccgagc ccgccgagcg 3720
cgtgagcctg cacatctgcc accccgaccg cgccacgctg acgcacgcct ccgtgctcgt 3780
tggcgcgggg ctcgctgcca ggcgcgtcag cccccctccg gccgagcccc tcgcatcttg 3840
ccccgccggc gacccgggcc ggccggctca gcgcagcgcg tcgcccccag cgaaacccct 3900
tggggatgcc gccgcgcccg agccccgcgg atgccagggg tgcgaactct gccggtacac 3960
gcgcgtcact aatgaccgcg cctatgtcaa cctgtggctc gagcgcgacc gcggcgccac 4020
cagctgggcg atgcgcattc ccgaggtggt tgtctacggg ccggagcacc tcgccacgca 4080
ttttccattg aaccactaca gtgtgctcaa gcccgcggag gtcaggcccc cgcgaggcat 4140
gtgcgggagt gacatgtggc gctgccgcgg ctggcagggc atgccgcagg tgcggtgcac 4200
cccctccaac gcccacgccg ccctgtgccg cacaggcgta ccccctcgag tgagcacgcg 4260
aggcggcgag ctagacccaa acacctgctg gctccgcgcc gccgccaacg ttgcgcaggc 4320
tgcgcgcgcc tgcggcgcct acacgagcgc cgggtgcccc aagtgcgcct acggccgcgc 4380
cttgagcgaa gcccgcactc atgaggactt tgccgccctg agccagcggt ggagcgcgag 4440
ccacgccgat gcctcccctg acggcaccgg agatcccctc gaccccctga tggagaccgt 4500
gggatgcgcc tgttcgcgcg tgtgggtcgg ctccgagcac gaggccccgc ccgaccacct 4560
cctggtgtcc ctccaccgcg cccccaatgg tccgtggggc gtagtgctcg aggtgcgcgc 4620
gcgccccgaa gggggcaacc ccaccggcca cttcgtttgc gcggtcggcg gcggcccacg 4680
ccgcgtctcg gaccgccccc atctttggct cgcggtcccc ctgtctcggg gcggcggcac 4740
ctgcgccgtg accgacgagg ggctggccca ggcgtactac gacgatctcg aggtgcgccg 4800
cctcggggat gacgccatgg cccgggcggc cctcgcatca gtccaacgcc ctcgcaaagg 4860
cccctacaat attagggtat ggaacatggc cgcaggcgct ggcaagacta cccgcatcct 4920
cgctgccttc acgcgcgaag acctctacgt ctgccccacc aatgcgctcc tgcacgagat 4980
tcaggccaaa ctccgcgcgc gcgacatcga catcaagaac gccgccacct acgagcgcgc 5040
gctcacgaaa ccgctcgccg cctaccgccg catctacatt gatgaggcgt tcaccctcgg 5100
cggcgagtac tgcgcgttcg ttgccagcca aaccacagcg gaggtgatct gcgtcggtga 5160
ccgagaccag tgcggcccac actacgccaa caactgccgc acccctgtcc ccgaccgctg 5220
gcctaccgag cgctcacgcc acacttggcg cttccccgac tgctgggccg cccgcctgcg 5280
cgcggggctc gattatgaca tcgagggcga gcgcatcggc accttcgcct gcaacctttg 5340
ggacggccgc caggtcgacc ttcacctcgc cttctcgcgc gaaaccgtgc gccgccttca 5400
cgaggctggc atacgcgcat acaccgtgcg cgaggcccag ggcatgagcg tcggcaccgc 5460
ctgcatccat gtaggcaggg acggcacgga cgttgccctg gcgctgacac gcgaccttgc 5520
catcgtcagc ctgacccggg cctccgacgc gctctacctc cacgagctcg aggacggctc 5580
actgcgcgct gcggggctca gcgcgttcct cgacgccggg gcactggcgg agctcaagga 5640
ggttcccgct ggcatcgacc gcgttgtcgc cgtcgaacag gcaccaccac cgttgccgcc 5700
cgccgacggc atccccgagg cccaagacgt gccacccttc tgtccccgca ctctggagga 5760
gctcgtcttc ggccgtgccg gccaccccca ttacgcggac ctcaaccgcg tgaccgaggg 5820
cgaacgggaa gtgcggtata tgcgcatctc gcgccacctg ctcaacaaga atcataccga 5880
gatgcctggg acggaacgcg tcctcagtgc cgtttgcgcc gtgcggcgct accgcgcggg 5940
cgaggatggg tcgaccctcc gcactgctgt ggcccgccag cacccgcgcc ctttccgcca 6000
gatcccaccc ccgcgcgtca ctgctggggt cgcccaggag tggcgcatga catacctgcg 6060
ggaacggatc gacctcactg atgtctacac gcagatgggc gtggctgcgc gggagctcac 6120
cgaccgctac gcgcgccgct accctgagat cttcgccggc atgtgtaccg cccagagcct 6180
gagcgtcccc gccttcctca aagccaccct gaagtgcgta gacgccgccc tcggccccag 6240
cgacaccgag gactgtcacg ccgcccaggg gaaagccggc cttgaaatcc gggcgtgggc 6300
caaggagtgg gttcaggtca tgtccccgca tttccgtgct atccagaaga tcatcatgcg 6360
cgccttgcgc ccgcaattcc ttgtggccgc aggccatacg gagcccgagg tcgatgcgtg 6420
gtggcaggct cactacacca ccaacgccat cgaggtcgac ttcaccgagt tcgacatgaa 6480
ccagaccctc gctactcggg acgtcgagct cgagattagc gccgctctct tgggcctccc 6540
ctgcgccgaa gactaccgtg cactccgcgc tggcagctac tgcaccctgc gcgaactggg 6600
taccactgag accggctgcg agcgcacaag cggcgagccc gccacgctgc tgcacaacac 6660
caccgtggcc atgtgcatgg ctatgcgcat ggtccccaaa ggcgtgcgct gggccgggat 6720
tttccagggc gacgacatgg tcatcttcct ccctgagggc gcgcgcagcg cggcacttaa 6780
gtggaccccc gccgaggtgg gcttgtttgg cttccacatc ccggtgaagc atgtgagcac 6840
ccctaccccc agtttctgcg ggcacgtcgg caccgcggcc ggcctcttcc atgatgtcat 6900
gcaccaggcg atcaaggtgc tctgccgccg tttcgaccca gacgtgcttg aagaacagca 6960
ggtggccctc ctcgaccgcc tccggggggt ctacgcggcc ctgcctgaca ccgttgccgc 7020
caatgccgcg tactacgact acagcgccga gcgcgtcctc gccattgtgc gcgaacttac 7080
cgcgtacgcg cgggggcgcg gcctcgacca cccggccacc atcggcgcgc tcgaggagat 7140
ccagaccccc tacgcgcgcg ccaatctcca cgacgccgac taacgcccct gtacgtgggg 7200
cctttaatct tacctactct aaccaggtca tcacccaccg ttgtttcgcc gcatctggtg 7260
ggtaccccac tcttgccatt cgggagagcc ccagggtgcc cgaatggctt ctactacccc 7320
catcaccatg gaggacctcc agaaggccct cgaggcacaa tcccgcgccc tgcgtgcgga 7380
actcgccgcc ggcgcctcgc agacgcgccg gccgcggccg ccgcgacagc gcgactccag 7440
cacctccgga gacgactccg gccgtgactc cggagggccc cgccgccgcc gcggcaaccg 7500
gggccgtggc cagcgcaggg actggtctag ggccccgccc cccccggaag agcggcaaga 7560
aagccgctcc cagactccgg ccccgaagcc atcgcgggcg ccgccacaac agcctcaacc 7620
cccgcgtatg caaaccgggc gtgggggctc tgccccgcgc cccgagctgg ggccaccgac 7680
caacccgttc caagcagccg tagcgcgtgg cctgcgcccg cctctccacg atcctgacac 7740
cgaggcaccc accgaggcct gcgtaacctc atggctttgg agcgaaggtg aaggcgcggt 7800
cttttaccgc gtcgatttac actttaccaa cctgggcacc cccccactcg acgaggacgg 7860
ccgctgggac cctgcgctca tgtacaaccc ttgcgggccc gagccgcccg ctcacgtcgt 7920
ccgtgcatac aatcaacctg ccggcgacgt caggggcgtt tggggtaaag gtgagcgcac 7980
ttacgctgag caggatttcc gcgttggcgg cacgcgctgg caccgactgc tgcgcatgcc 8040
agtgcgcggc ctcgacggcg acagcgcccc gctacccccc cacaccaccg aacgcatcga 8100
gacccgctcg gcgcgccatc cctggcgcat ccgtttcggc gctccccagg ccttccttgc 8160
cgggctcttg ctcgccgcgg tcgccgtagg caccgcgcgc gccgggctcc agccccgcgc 8220
tgacatggcg gcacccccag tgccgccgca gcccccccgt gcgcacgggc agcattacgg 8280
ccatcaccac catcagctgc cgttcctcgg gcacgacggc catcacggcg gcaccttgcg 8340
tgtcggccag catcaccgaa acgccagcga cgtgctgccc ggccactggc tccagggtgg 8400
ctggggttgc tacaacctga gcgactggca ccagggcact catgtctgtc acaccaagca 8460
catggacttc tggtgtgtgg agcacgaccg accgccgccc gcgactccga cgcctctcac 8520
caccgcggcg aatgccacaa ccgccgccac ccccgccacc gccccggccc cctgccacgc 8580
cggcctcaat gacagctgcg gcggcttcct gtcagggtgc gggccgatgc gcctgcgcca 8640
cggcgccgac acccggtgcg gtcggttgat ctgcgggctg tccaccaccg cccagtaccc 8700
gcctacccgg ttcggctgcg ccatgcggtg gggccttccc ccctgggaat tggtcgttct 8760
caccgcccgc cctgaagacg gctggacctg ccgcggcgtg cccgcccacc caggcacccg 8820
ctgccccgaa ctggtgagcc ccatgggacg cgcgacttgc tccccggcct cggcgctctg 8880
gctcgccaca gcgaacgcgc tgtctcttga tcacgccctc gcggccttcg tcctgctggt 8940
cccgtgggtc ctgatattca tggtgtgccg ccgcgcctgc cgccgccgcg gcgccgccgc 9000
cgcccttacc gcggtcgtcc tgcaggggta caaccccccc gcctacggcg aggaggcttt 9060
cacctacctc tgtactgcac cggggtgcgc cactcaaaca cctgtccccg tgcgcctcgc 9120
tggcgtccgc tttgagtcca agatcgtgga cggtggctgc tttgccccat gggacctcga 9180
ggccactgga gcctgcattt gcgagatccc caccgacgtc tcgtgcgagg gcttgggggc 9240
ctgggtaccc acagcccctt gcgcgcgcat ctggaacggc acacagcgcg catgcacctt 9300
ttgggctgtc aatgcctact cctctggtgg gtacgcgcag ctggcctctt acttcaaccc 9360
tggcggcagc tactacaagc agtaccaccc taccgcgtgc gaggttgaac ctgccttcgg 9420
ccacagcgac gcggcctgct ggggcttccc caccgacacc gtgatgagcg tgttcgccct 9480
tgccagctac gtccagcacc ctcacaagac cgtccgggtc aagttccata cagagaccag 9540
gaccgtctgg cagctctccg ttgccggcgt gtcgtgcaac gtcaccaccg aacacccgtt 9600
ctgcaacacg ccgcacggac aactcgaggt ccaggtcccg cccgaccccg gagacctggt 9660
tgagtacatc atgaattaca ccggcaatca gcagtcccgg tggggcctcg ggagcccgaa 9720
ttgccatggc cccgattggg cctccccggt ttgtcaacgc cattcccctg actgctcgcg 9780
gcttgtgggg gccacgccag agcgtccccg gctgcgcctg gttgatgctg acgaccccct 9840
gctgcgcact gcccctgggc ccggcgaggt gtgggtcacg cccgtcatag gctctcaggc 9900
gcgcaagtgc ggactccaca tacgcgccgg gccgtacggc catgccaccg tcgaaatgcc 9960
cgagtggatc cacgctcaca ccaccagcga cccctggcac ccaccgggcc ccttggggct 10020
caagtttaag acagttcgcc cggttgcgct accacgcgcg ttagcgccac cccgcaatgt 10080
gcgtgtgacc gggtgctacc agtgcggtac ccccgcgctg gtggaaggcc ttgcccccgg 10140
ggggggcaat tgccatctca ccgtcaatgg cgaggacgtc ggcgccttcc cccctgggaa 10200
gttcgttacc gccgccctcc tcaacacccc cccgccctac caagtcagct gcgggggcga 10260
gagcgatcgc gcgagcgcgc gggttattga ccccgccgcg caatcgttca ccggcgtggt 10320
gtatggcaca cacaccactg ctgtgtcgga gacccggcag acctgggcgg agtgggctgc 10380
cgcccattgg tggcagctca ctctgggcgc tatttgcacc ctcctactcg ctggcttact 10440
cgcttgctgt gccaaatgct tgtactactt gcgcggcgct atagcgccgc gctagtgggc 10500
ccccgcgcga aacccgcatt agcccactgg attcccgcac ctgttgctgc atagaaaaaa 10560
aaaaaaaaaa aaagaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc 10620
cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 10680
aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 10740
acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 10800
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 10860
gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 10920
caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 10980
tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 11040
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 11100
ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 11160
cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 11220
tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 11280
tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 11340
cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 11400
agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 11460
agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 11520
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 11580
aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 11640
ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 11700
gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 11760
taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 11820
tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 11880
tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 11940
gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 12000
gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 12060
ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 12120
cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 12180
tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 12240
cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 12300
agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 12360
cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 12420
aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 12480
aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 12540
gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 12600
gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 12660
tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 12720
ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 12780
aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 12840
tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 12900
gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 12960
cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 13020
gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 13080
aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 13140
caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 13200
ccagtgcc 13208
<210> 65
<211> 14234
<212> DNA
<213> Artificial Sequence
<220>
<223> pHS-717AG1-1005pMag-nMaghigh r2
<400> 65
actagtattt aggtgacact atagcaatgg gagctaacgg acctcgctta ggactcctat 60
tcccatggag aaactcctag atgaggttct tgcccccggt gggccttata acttaaccgt 120
cggcagttgg gtaagagacc atgtccgctc aattgtcgag ggcgcgtggg aagtgcgcga 180
tgtcgtcacc gctgcccaaa agcgggccat cgtagctgtg atacccagac ctgtattcac 240
gcagatgcag gttagtgatc acccagcact ccacgcaatt tcgcggtata cccgccgcca 300
ttggatcgag tggggcccta aagaagccct acacgtcctc atcgacccga gcccgggcct 360
gctccgcgag gtcgctcgcg ttgagcgccg ctgggtcgca ctgtgcctcc acaggacggc 420
acgcaaactc gccaccgccc tggccgagac ggccggcgag gcgtggcatg ccgactacgt 480
gtgcgcgctg cgtggcgcac cgagcggccc cttctacgtc caccctgagg acgtcccgca 540
cggcggccgc gccgtggcgg acagatgctt gctctactac acacccatgc agatgtgcga 600
gctgatgcgt gccattgacg ccaccttgct cgtggcggtt gacttgtggc cggtcgccct 660
tgcggctcat gtcggcgacg actgggacga cctgggcatc gcctggcacc tcgaccatga 720
cggcggctgc cccgccgact gccgcggagc cggcgctggg cccacgcccg gctacacccg 780
cccctgcacc acacgcatct accaagtcct gccggacacc gcccaccccg ggcgcctcta 840
ccggtgcggg ccccgcctgt ggacgcgcga ttgcgccgtg gccgaactct catgggaggt 900
tgcccagcac tgcgggcacc aggcgcgcgt gcgcgccgtg cgatgcaccc tccccatccg 960
ccacgtgcgc agcctccaac ccagcgcacg ggtccgactc ccggacctcg tccatctcgc 1020
cgaggtgggc cggtggcggt ggttcagcct cccccgcccc gtgttccagc gcatgctgtc 1080
ctactgcaag accctgagcc ccgacgcgta ctacagcgag cgtgtgttca agttcaagaa 1140
cgccctgagc cacagcatca cgctcgcggg caatgtgctg caagaggggt ggaagggcac 1200
gtgcgccgag gaagacgcgc tgtgcgcata cgtagccttc cgcgcgtggc agtctaacgc 1260
caggttggcg gggatcatga aaggcgctaa gcgctgcgct gccgactctt tgagcgtggc 1320
cggctggctg gacaccattt gggacgccat caagcggttc ttcggcagcg tgcccctcgc 1380
cgagcgcatg gaggagtggg aacaggacgc cgcggtcgcc gccttcgacc gcggccccct 1440
tgaggacggc gggcgccact tggacaccgt gcaacccccg aagtcgccgc cccgccctga 1500
gatcgccgcg acctggatcg tccacgcagc cagcgcagac cgccattgcg cgtgcgctcc 1560
ccgctgcgac gtcccgcgcg aacgtccttc cgcgcccgcc ggcccgcccg atgacgaggc 1620
gctcatcccg ccgtggctgt tcgccgagcg ccgtgccctc cgctgccgcg agtgggactt 1680
tgaggctctc cgcgcgcgcg ccgatacggc ggccgcgccc gccccgctgg ccccacgccc 1740
tgcgcggtac cccactgtgc tctaccgcca ccccgcccac cacggcccgt ggctcaccct 1800
tgacgagccg ggcgaggctg acgcggccct ggtcttatgc gacccgcttg gccagccgct 1860
ccggggccct gaacgccact tcgccgccgg cgcgcacatg tgcgcgcagg cgcgcgggct 1920
ccaggctttt gtccgtgttg tgcctccacc cgaacgcccc tgggccgacg ggggcgccag 1980
agcgtgggca aaattcttcc gcggctgcgc ctgggcgcag cgcttgctcg gcgagccggc 2040
agtgatgcac ctcccataca ccgatggtga cgtgccacag ctgatcgcgc tggccttgcg 2100
cacgctggcc cagcaggggg ccgccttggc actctcggtg cgagacctgc ccgggggtgc 2160
agcgttcgac gcgaatgcgg ttaccgccgc agtgcgcgct ggccccggcc agtccaagct 2220
tggtggttca ggaggaggat cgacccaagg aaccggtatg gtgagcgtga tcaagcccga 2280
gatgaagatc aagctgtgca tgaggggcac cgtgaacggc cacaacttcg tgatcgaggg 2340
cgagggcaag ggcaacccct acgagggcac ccagatcctg gacctgaacg tgaccgaggg 2400
cgcccccctg cccttcgcct acgacatcct gaccaccgtg ttccagtacg gcaacagggc 2460
cttcaccaag taccccgccg acatccagga ctacttcaag cagaccttcc ccgagggcta 2520
ccactgggag aggagcatga cctacgagga ccagggcatc tgcaccgcca ccagcaacat 2580
cagcatgagg ggcgactgct tcttctacga catcaggttc gacggcacca acttcccccc 2640
caacggcccc gtgatgcaga agaagaccct gaagtgggag cccagcaccg agaagatgta 2700
cgtggaggac ggcgtgctga agggcgacgt gaacatgagg ctgctgctgg agggcggcgg 2760
ccactacagg tgcgacttca agaccaccta caaggccaag aaggaggtga ggctgcccga 2820
cgcccacaag atcgaccaca ggatcgagat cctgaagcac gacaaggact acaacaaggt 2880
gaagctgtac gagaacgccg tggccaggta ctccatgctg cccagccagg ccaagaccgg 2940
taattccgct gacggcggcg gaggatcggg tggtagtaag cttgcggcca cgtcaccgcc 3000
acccggcgac cccccgccac cgcgccgcgc acggcgatcg caacggcact cggacgcccg 3060
cagcactccg ccctccgcgc ctgcgcgcga cccgccgccg cccgccccca gcccgcccgc 3120
gccaccccgc gcgggtgacc tggccccccc cattctcgcg gggccggcgg atcgcgcgcg 3180
tgacgccgag ctggaggtcg cctgcgaacc gagcggcccc cccgcgccaa ccaaggcaga 3240
cccagacagc gacatcgttg aaagttacgc ccgcgccgcc gggcccgtgc acctccgggt 3300
ccgtgacatc atggacccac cgcctgggtg caaggtcgtg gtcaacgccg ccaacgaggg 3360
gctgctggcc ggctttggcg tgtgtggtgc catctttgcc aacgccacgg cggcccttgc 3420
tgcagactgc cggcgcctcg ccccatgccc caccggcgag gcagtggcga cacccggcca 3480
cggctgcggg tacacccaca tcatccacgc cgttgccccg cggcgccctc gggaccccgc 3540
cgccctcgag gagggcgagg cactgctcga gcgcgcctac cgcagcatcg tcgcgctagc 3600
tgctgcgcgt aggtggacgt gtatcgcgtg ccccctcctc ggcgctggcg tctacggctg 3660
gtctgctgcg gagtccctcc gcgccgcgct tgcggccacg cgcgccgagc ccgccgagcg 3720
cgtgagcctg cacatctgcc accccgaccg cgccacgctg acgcacgcct ccgtgctcgt 3780
tggcgcgggg ctcgctgcca ggcgcgtcag cccccctccg gccgagcccc tcgcatcttg 3840
ccccgccaag cttagccata ctctttatgc ccccggtgga tatgacatta tgggatatct 3900
gaggcagatc aggaaccggc caaacccgca ggtggaactg ggccccgtgg atacatcctg 3960
cgccttgatt ctttgtgacc tgaaacagaa agacaccccg atagtttacg cgagtgaagc 4020
cttcctctac atgacaggtt acagcaacgc agaggtgctg ggccggaatt gccggtttct 4080
gcaaagccct gacggcatgg tgaagcccaa gagcacccgg aagtacgtgg atagtaacac 4140
aatcaatact atgcgcaagg caatcgacag gaatgccgag gtgcaggttg aagtagtcaa 4200
ttttaaaaag aatggacagc gatttgttaa tttcctgact atgatacctg ttagggacga 4260
aacaggcgag tatcgatact ctatgggatt ccagtgcgaa acagaaaccg gtaattccgc 4320
tgacggcggc ggaggatcgg gtggtagtgg tggttcagga ggaggatcga cccaaggagg 4380
atccatgcat actctttatg cccccggtgg atatgacatt atgggatatc tggaccagat 4440
cggcaaccgg ccaaacccgc aggtggaact gggccccgtg gatacatcct gcgccttgat 4500
tctttgtgac ctgaaacaga aagacacccc gatagtttac gcgagtgaag ccttcctcta 4560
catgacaggt tacagcaacg cagaggtgct gggccggaat tgccggtttc tgcaaagccc 4620
tgacggcatg gtgaagccca agagcacccg gaagtacgtg gatagtaaca caatcaatac 4680
tatccgcaag gcaatcgaca ggaatgccga ggtgcaggtt gaagtagtca attttaaaaa 4740
gaatggacag cgatttgtta atttcctgac tatcatacct gttagggacg aaacaggcga 4800
gtatcgatac tctatgggat tccagtgcga aacagaatat ccgtacgacg tcccagatta 4860
cgcgggcaag cttggcgacc cgggccggcc ggctcagcgc agcgcgtcgc ccccagcgaa 4920
accccttggg gatgccgccg cgcccgagcc ccgcggatgc caggggtgcg aactctgccg 4980
gtacacgcgc gtcactaatg accgcgccta tgtcaacctg tggctcgagc gcgaccgcgg 5040
cgccaccagc tgggcgatgc gcattcccga ggtggttgtc tacgggccgg agcacctcgc 5100
cacgcatttt ccattgaacc actacagtgt gctcaagccc gcggaggtca ggcccccgcg 5160
aggcatgtgc gggagtgaca tgtggcgctg ccgcggctgg cagggcatgc cgcaggtgcg 5220
gtgcaccccc tccaacgccc acgccgccct gtgccgcaca ggcgtacccc ctcgagtgag 5280
cacgcgaggc ggcgagctag acccaaacac ctgctggctc cgcgccgccg ccaacgttgc 5340
gcaggctgcg cgcgcctgcg gcgcctacac gagcgccggg tgccccaagt gcgcctacgg 5400
ccgcgccttg agcgaagccc gcactcatga ggactttgcc gccctgagcc agcggtggag 5460
cgcgagccac gccgatgcct cccctgacgg caccggagat cccctcgacc ccctgatgga 5520
gaccgtggga tgcgcctgtt cgcgcgtgtg ggtcggctcc gagcacgagg ccccgcccga 5580
ccacctcctg gtgtccctcc accgcgcccc caatggtccg tggggcgtag tgctcgaggt 5640
gcgcgcgcgc cccgaagggg gcaaccccac cggccacttc gtttgcgcgg tcggcggcgg 5700
cccacgccgc gtctcggacc gcccccatct ttggctcgcg gtccccctgt ctcggggcgg 5760
cggcacctgc gccgtgaccg acgaggggct ggcccaggcg tactacgacg atctcgaggt 5820
gcgccgcctc ggggatgacg ccatggcccg ggcggccctc gcatcagtcc aacgccctcg 5880
caaaggcccc tacaatatta gggtatggaa catggccgca ggcgctggca agactacccg 5940
catcctcgct gccttcacgc gcgaagacct ctacgtctgc cccaccaatg cgctcctgca 6000
cgagattcag gccaaactcc gcgcgcgcga catcgacatc aagaacgccg ccacctacga 6060
gcgcgcgctc acgaaaccgc tcgccgccta ccgccgcatc tacattgatg aggcgttcac 6120
cctcggcggc gagtactgcg cgttcgttgc cagccaaacc acagcggagg tgatctgcgt 6180
cggtgaccga gaccagtgcg gcccacacta cgccaacaac tgccgcaccc ctgtccccga 6240
ccgctggcct accgagcgct cacgccacac ttggcgcttc cccgactgct gggccgcccg 6300
cctgcgcgcg gggctcgatt atgacatcga gggcgagcgc atcggcacct tcgcctgcaa 6360
cctttgggac ggccgccagg tcgaccttca cctcgccttc tcgcgcgaaa ccgtgcgccg 6420
ccttcacgag gctggcatac gcgcatacac cgtgcgcgag gcccagggca tgagcgtcgg 6480
caccgcctgc atccatgtag gcagggacgg cacggacgtt gccctggcgc tgacacgcga 6540
ccttgccatc gtcagcctga cccgggcctc cgacgcgctc tacctccacg agctcgagga 6600
cggctcactg cgcgctgcgg ggctcagcgc gttcctcgac gccggggcac tggcggagct 6660
caaggaggtt cccgctggca tcgaccgcgt tgtcgccgtc gaacaggcac caccaccgtt 6720
gccgcccgcc gacggcatcc ccgaggccca agacgtgcca cccttctgtc cccgcactct 6780
ggaggagctc gtcttcggcc gtgccggcca cccccattac gcggacctca accgcgtgac 6840
cgagggcgaa cgggaagtgc ggtatatgcg catctcgcgc cacctgctca acaagaatca 6900
taccgagatg cctgggacgg aacgcgtcct cagtgccgtt tgcgccgtgc ggcgctaccg 6960
cgcgggcgag gatgggtcga ccctccgcac tgctgtggcc cgccagcacc cgcgcccttt 7020
ccgccagatc ccacccccgc gcgtcactgc tggggtcgcc caggagtggc gcatgacata 7080
cctgcgggaa cggatcgacc tcactgatgt ctacacgcag atgggcgtgg ctgcgcggga 7140
gctcaccgac cgctacgcgc gccgctaccc tgagatcttc gccggcatgt gtaccgccca 7200
gagcctgagc gtccccgcct tcctcaaagc caccctgaag tgcgtagacg ccgccctcgg 7260
ccccagcgac accgaggact gtcacgccgc ccaggggaaa gccggccttg aaatccgggc 7320
gtgggccaag gagtgggttc aggtcatgtc cccgcatttc cgtgctatcc agaagatcat 7380
catgcgcgcc ttgcgcccgc aattccttgt ggccgcaggc catacggagc ccgaggtcga 7440
tgcgtggtgg caggctcact acaccaccaa cgccatcgag gtcgacttca ccgagttcga 7500
catgaaccag accctcgcta ctcgggacgt cgagctcgag attagcgccg ctctcttggg 7560
cctcccctgc gccgaagact accgtgcact ccgcgctggc agctactgca ccctgcgcga 7620
actgggtacc actgagaccg gctgcgagcg cacaagcggc gagcccgcca cgctgctgca 7680
caacaccacc gtggccatgt gcatggctat gcgcatggtc cccaaaggcg tgcgctgggc 7740
cgggattttc cagggcgacg acatggtcat cttcctccct gagggcgcgc gcagcgcggc 7800
acttaagtgg acccccgccg aggtgggctt gtttggcttc cacatcccgg tgaagcatgt 7860
gagcacccct acccccagtt tctgcgggca cgtcggcacc gcggccggcc tcttccatga 7920
tgtcatgcac caggcgatca aggtgctctg ccgccgtttc gacccagacg tgcttgaaga 7980
acagcaggtg gccctcctcg accgcctccg gggggtctac gcggccctgc ctgacaccgt 8040
tgccgccaat gccgcgtact acgactacag cgccgagcgc gtcctcgcca ttgtgcgcga 8100
acttaccgcg tacgcgcggg ggcgcggcct cgaccacccg gccaccatcg gcgcgctcga 8160
ggagatccag accccctacg cgcgcgccaa tctccacgac gccgactaac gcccctgtac 8220
gtggggcctt taatcttacc tactctaacc aggtcatcac ccaccgttgt ttcgccgcat 8280
ctggtgggta ccccactctt gccattcggg agagccccag ggtgcccgaa tggcttctac 8340
tacccccatc accatggagg acctccagaa ggccctcgag gcacaatccc gcgccctgcg 8400
tgcggaactc gccgccggcg cctcgcagac gcgccggccg cggccgccgc gacagcgcga 8460
ctccagcacc tccggagacg actccggccg tgactccgga gggccccgcc gccgccgcgg 8520
caaccggggc cgtggccagc gcagggactg gtctagggcc ccgccccccc cggaagagcg 8580
gcaagaaagc cgctcccaga ctccggcccc gaagccatcg cgggcgccgc cacaacagcc 8640
tcaacccccg cgtatgcaaa ccgggcgtgg gggctctgcc ccgcgccccg agctggggcc 8700
accgaccaac ccgttccaag cagccgtagc gcgtggcctg cgcccgcctc tccacgatcc 8760
tgacaccgag gcacccaccg aggcctgcgt aacctcatgg ctttggagcg aaggtgaagg 8820
cgcggtcttt taccgcgtcg atttacactt taccaacctg ggcacccccc cactcgacga 8880
ggacggccgc tgggaccctg cgctcatgta caacccttgc gggcccgagc cgcccgctca 8940
cgtcgtccgt gcatacaatc aacctgccgg cgacgtcagg ggcgtttggg gtaaaggtga 9000
gcgcacttac gctgagcagg atttccgcgt tggcggcacg cgctggcacc gactgctgcg 9060
catgccagtg cgcggcctcg acggcgacag cgccccgcta cccccccaca ccaccgaacg 9120
catcgagacc cgctcggcgc gccatccctg gcgcatccgt ttcggcgctc cccaggcctt 9180
ccttgccggg ctcttgctcg ccgcggtcgc cgtaggcacc gcgcgcgccg ggctccagcc 9240
ccgcgctgac atggcggcac ccccagtgcc gccgcagccc ccccgtgcgc acgggcagca 9300
ttacggccat caccaccatc agctgccgtt cctcgggcac gacggccatc acggcggcac 9360
cttgcgtgtc ggccagcatc accgaaacgc cagcgacgtg ctgcccggcc actggctcca 9420
gggtggctgg ggttgctaca acctgagcga ctggcaccag ggcactcatg tctgtcacac 9480
caagcacatg gacttctggt gtgtggagca cgaccgaccg ccgcccgcga ctccgacgcc 9540
tctcaccacc gcggcgaatg ccacaaccgc cgccaccccc gccaccgccc cggccccctg 9600
ccacgccggc ctcaatgaca gctgcggcgg cttcctgtca gggtgcgggc cgatgcgcct 9660
gcgccacggc gccgacaccc ggtgcggtcg gttgatctgc gggctgtcca ccaccgccca 9720
gtacccgcct acccggttcg gctgcgccat gcggtggggc cttcccccct gggaattggt 9780
cgttctcacc gcccgccctg aagacggctg gacctgccgc ggcgtgcccg cccacccagg 9840
cacccgctgc cccgaactgg tgagccccat gggacgcgcg acttgctccc cggcctcggc 9900
gctctggctc gccacagcga acgcgctgtc tcttgatcac gccctcgcgg ccttcgtcct 9960
gctggtcccg tgggtcctga tattcatggt gtgccgccgc gcctgccgcc gccgcggcgc 10020
cgccgccgcc cttaccgcgg tcgtcctgca ggggtacaac ccccccgcct acggcgagga 10080
ggctttcacc tacctctgta ctgcaccggg gtgcgccact caaacacctg tccccgtgcg 10140
cctcgctggc gtccgctttg agtccaagat cgtggacggt ggctgctttg ccccatggga 10200
cctcgaggcc actggagcct gcatttgcga gatccccacc gacgtctcgt gcgagggctt 10260
gggggcctgg gtacccacag ccccttgcgc gcgcatctgg aacggcacac agcgcgcatg 10320
caccttttgg gctgtcaatg cctactcctc tggtgggtac gcgcagctgg cctcttactt 10380
caaccctggc ggcagctact acaagcagta ccaccctacc gcgtgcgagg ttgaacctgc 10440
cttcggccac agcgacgcgg cctgctgggg cttccccacc gacaccgtga tgagcgtgtt 10500
cgcccttgcc agctacgtcc agcaccctca caagaccgtc cgggtcaagt tccatacaga 10560
gaccaggacc gtctggcagc tctccgttgc cggcgtgtcg tgcaacgtca ccaccgaaca 10620
cccgttctgc aacacgccgc acggacaact cgaggtccag gtcccgcccg accccggaga 10680
cctggttgag tacatcatga attacaccgg caatcagcag tcccggtggg gcctcgggag 10740
cccgaattgc catggccccg attgggcctc cccggtttgt caacgccatt cccctgactg 10800
ctcgcggctt gtgggggcca cgccagagcg tccccggctg cgcctggttg atgctgacga 10860
ccccctgctg cgcactgccc ctgggcccgg cgaggtgtgg gtcacgcccg tcataggctc 10920
tcaggcgcgc aagtgcggac tccacatacg cgccgggccg tacggccatg ccaccgtcga 10980
aatgcccgag tggatccacg ctcacaccac cagcgacccc tggcacccac cgggcccctt 11040
ggggctcaag tttaagacag ttcgcccggt tgcgctacca cgcgcgttag cgccaccccg 11100
caatgtgcgt gtgaccgggt gctaccagtg cggtaccccc gcgctggtgg aaggccttgc 11160
ccccgggggg ggcaattgcc atctcaccgt caatggcgag gacgtcggcg ccttcccccc 11220
tgggaagttc gttaccgccg ccctcctcaa cacccccccg ccctaccaag tcagctgcgg 11280
gggcgagagc gatcgcgcga gcgcgcgggt tattgacccc gccgcgcaat cgttcaccgg 11340
cgtggtgtat ggcacacaca ccactgctgt gtcggagacc cggcagacct gggcggagtg 11400
ggctgccgcc cattggtggc agctcactct gggcgctatt tgcaccctcc tactcgctgg 11460
cttactcgct tgctgtgcca aatgcttgta ctacttgcgc ggcgctatag cgccgcgcta 11520
gtgggccccc gcgcgaaacc cgcattagcc cactggattc ccgcacctgt tgctgcatag 11580
aaaaaaaaaa aaaaaaaaag aattcgtaat catggtcata gctgtttcct gtgtgaaatt 11640
gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 11700
gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 11760
cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 11820
tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 11880
tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 11940
ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 12000
ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 12060
gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 12120
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 12180
ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 12240
tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 12300
gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 12360
tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 12420
tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 12480
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 12540
ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 12600
ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 12660
gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 12720
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 12780
aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 12840
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 12900
ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 12960
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 13020
ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 13080
ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 13140
ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 13200
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 13260
ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 13320
ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 13380
gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 13440
ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 13500
cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 13560
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 13620
aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 13680
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 13740
gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 13800
cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 13860
aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 13920
ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 13980
actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 14040
acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 14100
gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 14160
gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 14220
cgacggccag tgcc 14234
<210> 66
<211> 14234
<212> DNA
<213> Artificial Sequence
<220>
<223> pHS-717pMag-nMaghigh-1005AG1 r3
<400> 66
actagtattt aggtgacact atagcaatgg gagctaacgg acctcgctta ggactcctat 60
tcccatggag aaactcctag atgaggttct tgcccccggt gggccttata acttaaccgt 120
cggcagttgg gtaagagacc atgtccgctc aattgtcgag ggcgcgtggg aagtgcgcga 180
tgtcgtcacc gctgcccaaa agcgggccat cgtagctgtg atacccagac ctgtattcac 240
gcagatgcag gttagtgatc acccagcact ccacgcaatt tcgcggtata cccgccgcca 300
ttggatcgag tggggcccta aagaagccct acacgtcctc atcgacccga gcccgggcct 360
gctccgcgag gtcgctcgcg ttgagcgccg ctgggtcgca ctgtgcctcc acaggacggc 420
acgcaaactc gccaccgccc tggccgagac ggccggcgag gcgtggcatg ccgactacgt 480
gtgcgcgctg cgtggcgcac cgagcggccc cttctacgtc caccctgagg acgtcccgca 540
cggcggccgc gccgtggcgg acagatgctt gctctactac acacccatgc agatgtgcga 600
gctgatgcgt gccattgacg ccaccttgct cgtggcggtt gacttgtggc cggtcgccct 660
tgcggctcat gtcggcgacg actgggacga cctgggcatc gcctggcacc tcgaccatga 720
cggcggctgc cccgccgact gccgcggagc cggcgctggg cccacgcccg gctacacccg 780
cccctgcacc acacgcatct accaagtcct gccggacacc gcccaccccg ggcgcctcta 840
ccggtgcggg ccccgcctgt ggacgcgcga ttgcgccgtg gccgaactct catgggaggt 900
tgcccagcac tgcgggcacc aggcgcgcgt gcgcgccgtg cgatgcaccc tccccatccg 960
ccacgtgcgc agcctccaac ccagcgcacg ggtccgactc ccggacctcg tccatctcgc 1020
cgaggtgggc cggtggcggt ggttcagcct cccccgcccc gtgttccagc gcatgctgtc 1080
ctactgcaag accctgagcc ccgacgcgta ctacagcgag cgtgtgttca agttcaagaa 1140
cgccctgagc cacagcatca cgctcgcggg caatgtgctg caagaggggt ggaagggcac 1200
gtgcgccgag gaagacgcgc tgtgcgcata cgtagccttc cgcgcgtggc agtctaacgc 1260
caggttggcg gggatcatga aaggcgctaa gcgctgcgct gccgactctt tgagcgtggc 1320
cggctggctg gacaccattt gggacgccat caagcggttc ttcggcagcg tgcccctcgc 1380
cgagcgcatg gaggagtggg aacaggacgc cgcggtcgcc gccttcgacc gcggccccct 1440
tgaggacggc gggcgccact tggacaccgt gcaacccccg aagtcgccgc cccgccctga 1500
gatcgccgcg acctggatcg tccacgcagc cagcgcagac cgccattgcg cgtgcgctcc 1560
ccgctgcgac gtcccgcgcg aacgtccttc cgcgcccgcc ggcccgcccg atgacgaggc 1620
gctcatcccg ccgtggctgt tcgccgagcg ccgtgccctc cgctgccgcg agtgggactt 1680
tgaggctctc cgcgcgcgcg ccgatacggc ggccgcgccc gccccgctgg ccccacgccc 1740
tgcgcggtac cccactgtgc tctaccgcca ccccgcccac cacggcccgt ggctcaccct 1800
tgacgagccg ggcgaggctg acgcggccct ggtcttatgc gacccgcttg gccagccgct 1860
ccggggccct gaacgccact tcgccgccgg cgcgcacatg tgcgcgcagg cgcgcgggct 1920
ccaggctttt gtccgtgttg tgcctccacc cgaacgcccc tgggccgacg ggggcgccag 1980
agcgtgggca aaattcttcc gcggctgcgc ctgggcgcag cgcttgctcg gcgagccggc 2040
agtgatgcac ctcccataca ccgatggtga cgtgccacag ctgatcgcgc tggccttgcg 2100
cacgctggcc cagcaggggg ccgccttggc actctcggtg cgagacctgc ccgggggtgc 2160
agcgttcgac gcgaatgcgg ttaccgccgc agtgcgcgct ggccccggcc agtccaagct 2220
tagccatact ctttatgccc ccggtggata tgacattatg ggatatctgg accagatcgg 2280
caaccggcca aacccgcagg tggaactggg ccccgtggat acatcctgcg ccttgattct 2340
ttgtgacctg aaacagaaag acaccccgat agtttacgcg agtgaagcct tcctctacat 2400
gacaggttac agcaacgcag aggtgctggg ccggaattgc cggtttctgc aaagccctga 2460
cggcatggtg aagcccaaga gcacccggaa gtacgtggat agtaacacaa tcaatactat 2520
gcgcaaggca atcgacagga atgccgaggt gcaggttgaa gtagtcaatt ttaaaaagaa 2580
tggacagcga tttgttaatt tcctgactat gatacctgtt agggacgaaa caggcgagta 2640
tcgatactct atgggattcc agtgcgaaac agaaaccggt aattccgctg acggcggcgg 2700
aggatcgggt ggtagtggtg gttcaggagg aggatcgacc caaggaggat ccatgcatac 2760
tctttatgcc cccggtggat atgacattat gggatatctg gaccagatcg gcaaccggcc 2820
aaacccgcag gtggaactgg gccccgtgga tacatcctgc gccttgattc tttgtgacct 2880
gaaacagaaa gacaccccga tagtttacgc gagtgaagcc ttcctctaca tgacaggtta 2940
cagcaacgca gaggtgctgg gccggaattg ccggtttctg caaagccctg acggcatggt 3000
gaagcccaag agcacccgga agtacgtgga tagtaacaca atcaatacta tccgcaaggc 3060
aatcgacagg aatgccgagg tgcaggttga agtagtcaat tttaaaaaga atggacagcg 3120
atttgttaat ttcctgacta tcatacctgt tagggacgaa acaggcgagt atcgatactc 3180
tatgggattc cagtgcgaaa cagaatatcc gtacgacgtc ccagattacg cgggcaagct 3240
tgcggccacg tcaccgccac ccggcgaccc cccgccaccg cgccgcgcac ggcgatcgca 3300
acggcactcg gacgcccgca gcactccgcc ctccgcgcct gcgcgcgacc cgccgccgcc 3360
cgcccccagc ccgcccgcgc caccccgcgc gggtgacctg gcccccccca ttctcgcggg 3420
gccggcggat cgcgcgcgtg acgccgagct ggaggtcgcc tgcgaaccga gcggcccccc 3480
cgcgccaacc aaggcagacc cagacagcga catcgttgaa agttacgccc gcgccgccgg 3540
gcccgtgcac ctccgggtcc gtgacatcat ggacccaccg cctgggtgca aggtcgtggt 3600
caacgccgcc aacgaggggc tgctggccgg ctttggcgtg tgtggtgcca tctttgccaa 3660
cgccacggcg gcccttgctg cagactgccg gcgcctcgcc ccatgcccca ccggcgaggc 3720
agtggcgaca cccggccacg gctgcgggta cacccacatc atccacgccg ttgccccgcg 3780
gcgccctcgg gaccccgccg ccctcgagga gggcgaggca ctgctcgagc gcgcctaccg 3840
cagcatcgtc gcgctagctg ctgcgcgtag gtggacgtgt atcgcgtgcc ccctcctcgg 3900
cgctggcgtc tacggctggt ctgctgcgga gtccctccgc gccgcgcttg cggccacgcg 3960
cgccgagccc gccgagcgcg tgagcctgca catctgccac cccgaccgcg ccacgctgac 4020
gcacgcctcc gtgctcgttg gcgcggggct cgctgccagg cgcgtcagcc cccctccggc 4080
cgagcccctc gcatcttgcc ccgccaagct tggtggttca ggaggaggat cgacccaagg 4140
aaccggtatg gtgagcgtga tcaagcccga gatgaagatc aagctgtgca tgaggggcac 4200
cgtgaacggc cacaacttcg tgatcgaggg cgagggcaag ggcaacccct acgagggcac 4260
ccagatcctg gacctgaacg tgaccgaggg cgcccccctg cccttcgcct acgacatcct 4320
gaccaccgtg ttccagtacg gcaacagggc cttcaccaag taccccgccg acatccagga 4380
ctacttcaag cagaccttcc ccgagggcta ccactgggag aggagcatga cctacgagga 4440
ccagggcatc tgcaccgcca ccagcaacat cagcatgagg ggcgactgct tcttctacga 4500
catcaggttc gacggcacca acttcccccc caacggcccc gtgatgcaga agaagaccct 4560
gaagtgggag cccagcaccg agaagatgta cgtggaggac ggcgtgctga agggcgacgt 4620
gaacatgagg ctgctgctgg agggcggcgg ccactacagg tgcgacttca agaccaccta 4680
caaggccaag aaggaggtga ggctgcccga cgcccacaag atcgaccaca ggatcgagat 4740
cctgaagcac gacaaggact acaacaaggt gaagctgtac gagaacgccg tggccaggta 4800
ctccatgctg cccagccagg ccaagaccgg taattccgct gacggcggcg gaggatcggg 4860
tggtagtaag cttggcgacc cgggccggcc ggctcagcgc agcgcgtcgc ccccagcgaa 4920
accccttggg gatgccgccg cgcccgagcc ccgcggatgc caggggtgcg aactctgccg 4980
gtacacgcgc gtcactaatg accgcgccta tgtcaacctg tggctcgagc gcgaccgcgg 5040
cgccaccagc tgggcgatgc gcattcccga ggtggttgtc tacgggccgg agcacctcgc 5100
cacgcatttt ccattgaacc actacagtgt gctcaagccc gcggaggtca ggcccccgcg 5160
aggcatgtgc gggagtgaca tgtggcgctg ccgcggctgg cagggcatgc cgcaggtgcg 5220
gtgcaccccc tccaacgccc acgccgccct gtgccgcaca ggcgtacccc ctcgagtgag 5280
cacgcgaggc ggcgagctag acccaaacac ctgctggctc cgcgccgccg ccaacgttgc 5340
gcaggctgcg cgcgcctgcg gcgcctacac gagcgccggg tgccccaagt gcgcctacgg 5400
ccgcgccttg agcgaagccc gcactcatga ggactttgcc gccctgagcc agcggtggag 5460
cgcgagccac gccgatgcct cccctgacgg caccggagat cccctcgacc ccctgatgga 5520
gaccgtggga tgcgcctgtt cgcgcgtgtg ggtcggctcc gagcacgagg ccccgcccga 5580
ccacctcctg gtgtccctcc accgcgcccc caatggtccg tggggcgtag tgctcgaggt 5640
gcgcgcgcgc cccgaagggg gcaaccccac cggccacttc gtttgcgcgg tcggcggcgg 5700
cccacgccgc gtctcggacc gcccccatct ttggctcgcg gtccccctgt ctcggggcgg 5760
cggcacctgc gccgtgaccg acgaggggct ggcccaggcg tactacgacg atctcgaggt 5820
gcgccgcctc ggggatgacg ccatggcccg ggcggccctc gcatcagtcc aacgccctcg 5880
caaaggcccc tacaatatta gggtatggaa catggccgca ggcgctggca agactacccg 5940
catcctcgct gccttcacgc gcgaagacct ctacgtctgc cccaccaatg cgctcctgca 6000
cgagattcag gccaaactcc gcgcgcgcga catcgacatc aagaacgccg ccacctacga 6060
gcgcgcgctc acgaaaccgc tcgccgccta ccgccgcatc tacattgatg aggcgttcac 6120
cctcggcggc gagtactgcg cgttcgttgc cagccaaacc acagcggagg tgatctgcgt 6180
cggtgaccga gaccagtgcg gcccacacta cgccaacaac tgccgcaccc ctgtccccga 6240
ccgctggcct accgagcgct cacgccacac ttggcgcttc cccgactgct gggccgcccg 6300
cctgcgcgcg gggctcgatt atgacatcga gggcgagcgc atcggcacct tcgcctgcaa 6360
cctttgggac ggccgccagg tcgaccttca cctcgccttc tcgcgcgaaa ccgtgcgccg 6420
ccttcacgag gctggcatac gcgcatacac cgtgcgcgag gcccagggca tgagcgtcgg 6480
caccgcctgc atccatgtag gcagggacgg cacggacgtt gccctggcgc tgacacgcga 6540
ccttgccatc gtcagcctga cccgggcctc cgacgcgctc tacctccacg agctcgagga 6600
cggctcactg cgcgctgcgg ggctcagcgc gttcctcgac gccggggcac tggcggagct 6660
caaggaggtt cccgctggca tcgaccgcgt tgtcgccgtc gaacaggcac caccaccgtt 6720
gccgcccgcc gacggcatcc ccgaggccca agacgtgcca cccttctgtc cccgcactct 6780
ggaggagctc gtcttcggcc gtgccggcca cccccattac gcggacctca accgcgtgac 6840
cgagggcgaa cgggaagtgc ggtatatgcg catctcgcgc cacctgctca acaagaatca 6900
taccgagatg cctgggacgg aacgcgtcct cagtgccgtt tgcgccgtgc ggcgctaccg 6960
cgcgggcgag gatgggtcga ccctccgcac tgctgtggcc cgccagcacc cgcgcccttt 7020
ccgccagatc ccacccccgc gcgtcactgc tggggtcgcc caggagtggc gcatgacata 7080
cctgcgggaa cggatcgacc tcactgatgt ctacacgcag atgggcgtgg ctgcgcggga 7140
gctcaccgac cgctacgcgc gccgctaccc tgagatcttc gccggcatgt gtaccgccca 7200
gagcctgagc gtccccgcct tcctcaaagc caccctgaag tgcgtagacg ccgccctcgg 7260
ccccagcgac accgaggact gtcacgccgc ccaggggaaa gccggccttg aaatccgggc 7320
gtgggccaag gagtgggttc aggtcatgtc cccgcatttc cgtgctatcc agaagatcat 7380
catgcgcgcc ttgcgcccgc aattccttgt ggccgcaggc catacggagc ccgaggtcga 7440
tgcgtggtgg caggctcact acaccaccaa cgccatcgag gtcgacttca ccgagttcga 7500
catgaaccag accctcgcta ctcgggacgt cgagctcgag attagcgccg ctctcttggg 7560
cctcccctgc gccgaagact accgtgcact ccgcgctggc agctactgca ccctgcgcga 7620
actgggtacc actgagaccg gctgcgagcg cacaagcggc gagcccgcca cgctgctgca 7680
caacaccacc gtggccatgt gcatggctat gcgcatggtc cccaaaggcg tgcgctgggc 7740
cgggattttc cagggcgacg acatggtcat cttcctccct gagggcgcgc gcagcgcggc 7800
acttaagtgg acccccgccg aggtgggctt gtttggcttc cacatcccgg tgaagcatgt 7860
gagcacccct acccccagtt tctgcgggca cgtcggcacc gcggccggcc tcttccatga 7920
tgtcatgcac caggcgatca aggtgctctg ccgccgtttc gacccagacg tgcttgaaga 7980
acagcaggtg gccctcctcg accgcctccg gggggtctac gcggccctgc ctgacaccgt 8040
tgccgccaat gccgcgtact acgactacag cgccgagcgc gtcctcgcca ttgtgcgcga 8100
acttaccgcg tacgcgcggg ggcgcggcct cgaccacccg gccaccatcg gcgcgctcga 8160
ggagatccag accccctacg cgcgcgccaa tctccacgac gccgactaac gcccctgtac 8220
gtggggcctt taatcttacc tactctaacc aggtcatcac ccaccgttgt ttcgccgcat 8280
ctggtgggta ccccactctt gccattcggg agagccccag ggtgcccgaa tggcttctac 8340
tacccccatc accatggagg acctccagaa ggccctcgag gcacaatccc gcgccctgcg 8400
tgcggaactc gccgccggcg cctcgcagac gcgccggccg cggccgccgc gacagcgcga 8460
ctccagcacc tccggagacg actccggccg tgactccgga gggccccgcc gccgccgcgg 8520
caaccggggc cgtggccagc gcagggactg gtctagggcc ccgccccccc cggaagagcg 8580
gcaagaaagc cgctcccaga ctccggcccc gaagccatcg cgggcgccgc cacaacagcc 8640
tcaacccccg cgtatgcaaa ccgggcgtgg gggctctgcc ccgcgccccg agctggggcc 8700
accgaccaac ccgttccaag cagccgtagc gcgtggcctg cgcccgcctc tccacgatcc 8760
tgacaccgag gcacccaccg aggcctgcgt aacctcatgg ctttggagcg aaggtgaagg 8820
cgcggtcttt taccgcgtcg atttacactt taccaacctg ggcacccccc cactcgacga 8880
ggacggccgc tgggaccctg cgctcatgta caacccttgc gggcccgagc cgcccgctca 8940
cgtcgtccgt gcatacaatc aacctgccgg cgacgtcagg ggcgtttggg gtaaaggtga 9000
gcgcacttac gctgagcagg atttccgcgt tggcggcacg cgctggcacc gactgctgcg 9060
catgccagtg cgcggcctcg acggcgacag cgccccgcta cccccccaca ccaccgaacg 9120
catcgagacc cgctcggcgc gccatccctg gcgcatccgt ttcggcgctc cccaggcctt 9180
ccttgccggg ctcttgctcg ccgcggtcgc cgtaggcacc gcgcgcgccg ggctccagcc 9240
ccgcgctgac atggcggcac ccccagtgcc gccgcagccc ccccgtgcgc acgggcagca 9300
ttacggccat caccaccatc agctgccgtt cctcgggcac gacggccatc acggcggcac 9360
cttgcgtgtc ggccagcatc accgaaacgc cagcgacgtg ctgcccggcc actggctcca 9420
gggtggctgg ggttgctaca acctgagcga ctggcaccag ggcactcatg tctgtcacac 9480
caagcacatg gacttctggt gtgtggagca cgaccgaccg ccgcccgcga ctccgacgcc 9540
tctcaccacc gcggcgaatg ccacaaccgc cgccaccccc gccaccgccc cggccccctg 9600
ccacgccggc ctcaatgaca gctgcggcgg cttcctgtca gggtgcgggc cgatgcgcct 9660
gcgccacggc gccgacaccc ggtgcggtcg gttgatctgc gggctgtcca ccaccgccca 9720
gtacccgcct acccggttcg gctgcgccat gcggtggggc cttcccccct gggaattggt 9780
cgttctcacc gcccgccctg aagacggctg gacctgccgc ggcgtgcccg cccacccagg 9840
cacccgctgc cccgaactgg tgagccccat gggacgcgcg acttgctccc cggcctcggc 9900
gctctggctc gccacagcga acgcgctgtc tcttgatcac gccctcgcgg ccttcgtcct 9960
gctggtcccg tgggtcctga tattcatggt gtgccgccgc gcctgccgcc gccgcggcgc 10020
cgccgccgcc cttaccgcgg tcgtcctgca ggggtacaac ccccccgcct acggcgagga 10080
ggctttcacc tacctctgta ctgcaccggg gtgcgccact caaacacctg tccccgtgcg 10140
cctcgctggc gtccgctttg agtccaagat cgtggacggt ggctgctttg ccccatggga 10200
cctcgaggcc actggagcct gcatttgcga gatccccacc gacgtctcgt gcgagggctt 10260
gggggcctgg gtacccacag ccccttgcgc gcgcatctgg aacggcacac agcgcgcatg 10320
caccttttgg gctgtcaatg cctactcctc tggtgggtac gcgcagctgg cctcttactt 10380
caaccctggc ggcagctact acaagcagta ccaccctacc gcgtgcgagg ttgaacctgc 10440
cttcggccac agcgacgcgg cctgctgggg cttccccacc gacaccgtga tgagcgtgtt 10500
cgcccttgcc agctacgtcc agcaccctca caagaccgtc cgggtcaagt tccatacaga 10560
gaccaggacc gtctggcagc tctccgttgc cggcgtgtcg tgcaacgtca ccaccgaaca 10620
cccgttctgc aacacgccgc acggacaact cgaggtccag gtcccgcccg accccggaga 10680
cctggttgag tacatcatga attacaccgg caatcagcag tcccggtggg gcctcgggag 10740
cccgaattgc catggccccg attgggcctc cccggtttgt caacgccatt cccctgactg 10800
ctcgcggctt gtgggggcca cgccagagcg tccccggctg cgcctggttg atgctgacga 10860
ccccctgctg cgcactgccc ctgggcccgg cgaggtgtgg gtcacgcccg tcataggctc 10920
tcaggcgcgc aagtgcggac tccacatacg cgccgggccg tacggccatg ccaccgtcga 10980
aatgcccgag tggatccacg ctcacaccac cagcgacccc tggcacccac cgggcccctt 11040
ggggctcaag tttaagacag ttcgcccggt tgcgctacca cgcgcgttag cgccaccccg 11100
caatgtgcgt gtgaccgggt gctaccagtg cggtaccccc gcgctggtgg aaggccttgc 11160
ccccgggggg ggcaattgcc atctcaccgt caatggcgag gacgtcggcg ccttcccccc 11220
tgggaagttc gttaccgccg ccctcctcaa cacccccccg ccctaccaag tcagctgcgg 11280
gggcgagagc gatcgcgcga gcgcgcgggt tattgacccc gccgcgcaat cgttcaccgg 11340
cgtggtgtat ggcacacaca ccactgctgt gtcggagacc cggcagacct gggcggagtg 11400
ggctgccgcc cattggtggc agctcactct gggcgctatt tgcaccctcc tactcgctgg 11460
cttactcgct tgctgtgcca aatgcttgta ctacttgcgc ggcgctatag cgccgcgcta 11520
gtgggccccc gcgcgaaacc cgcattagcc cactggattc ccgcacctgt tgctgcatag 11580
aaaaaaaaaa aaaaaaaaag aattcgtaat catggtcata gctgtttcct gtgtgaaatt 11640
gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 11700
gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 11760
cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 11820
tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 11880
tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 11940
ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 12000
ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 12060
gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 12120
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 12180
ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 12240
tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 12300
gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 12360
tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 12420
tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 12480
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 12540
ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 12600
ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 12660
gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 12720
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 12780
aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 12840
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 12900
ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 12960
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 13020
ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 13080
ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 13140
ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 13200
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 13260
ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 13320
ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 13380
gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 13440
ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 13500
cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 13560
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 13620
aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 13680
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 13740
gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 13800
cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 13860
aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 13920
ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 13980
actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 14040
acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 14100
gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 14160
gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 14220
cgacggccag tgcc 14234
<210> 67
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> 717正向引物
<400> 67
cggccagtcc aagcttagcc atactcttta tgcccccggt 40
<210> 68
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> 1005正向引物
<400> 68
ttgccccgcc aagcttagcc atactcttta tgcccccggt 40
<210> 69
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> 717反向引物
<400> 69
acgtggccgc aagcttgccc gcgtaatctg ggacg 35
<210> 70
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> 1005反向引物
<400> 70
ccgggtcgcc aagcttgccc gcgtaatctg ggac 34
<210> 71
<211> 11261
<212> DNA
<213> Artificial Sequence
<220>
<223> wt P150 r4
<400> 71
actagtattt aggtgacact atagcaatgg gagctaacgg acctcgctta ggactcctat 60
tcccatggag aaactcctag atgaggttct tgcccccggt gggccttata acttaaccgt 120
cggcagttgg gtaagagacc atgtccgctc aattgtcgag ggcgcgtggg aagtgcgcga 180
tgtcgtcacc gctgcccaaa agcgggccat cgtagctgtg atacccagac ctgtattcac 240
gcagatgcag gttagtgatc acccagcact ccacgcaatt tcgcggtata cccgccgcca 300
ttggatcgag tggggcccta aagaagccct acacgtcctc atcgacccga gcccgggcct 360
gctccgcgag gtcgctcgcg ttgagcgccg ctgggtcgca ctgtgcctcc acaggacggc 420
acgcaaactc gccaccgccc tggccgagac ggccggcgag gcgtggcatg ccgactacgt 480
gtgcgcgctg cgtggcgcac cgagcggccc cttctacgtc caccctgagg acgtcccgca 540
cggcggccgc gccgtggcgg acagatgctt gctctactac acacccatgc agatgtgcga 600
gctgatgcgt gccattgacg ccaccttgct cgtggcggtt gacttgtggc cggtcgccct 660
tgcggctcat gtcggcgacg actgggacga cctgggcatc gcctggcacc tcgaccatga 720
cggcggctgc cccgccgact gccgcggagc cggcgctggg cccacgcccg gctacacccg 780
cccctgcacc acacgcatct accaagtcct gccggacacc gcccaccccg ggcgcctcta 840
ccggtgcggg ccccgcctgt ggacgcgcga ttgcgccgtg gccgaactct catgggaggt 900
tgcccagcac tgcgggcacc aggcgcgcgt gcgcgccgtg cgatgcaccc tccccatccg 960
ccacgtgcgc agcctccaac ccagcgcacg ggtccgactc ccggacctcg tccatctcgc 1020
cgaggtgggc cggtggcggt ggttcagcct cccccgcccc gtgttccagc gcatgctgtc 1080
ctactgcaag accctgagcc ccgacgcgta ctacagcgag cgtgtgttca agttcaagaa 1140
cgccctgagc cacagcatca cgctcgcggg caatgtgctg caagaggggt ggaagggcac 1200
gtgcgccgag gaagacgcgc tgtgcgcata cgtagccttc cgcgcgtggc agtctaacgc 1260
caggttggcg gggatcatga aaggcgctaa gcgctgcgct gccgactctt tgagcgtggc 1320
cggctggctg gacaccattt gggacgccat caagcggttc ttcggcagcg tgcccctcgc 1380
cgagcgcatg gaggagtggg aacaggacgc cgcggtcgcc gccttcgacc gcggccccct 1440
tgaggacggc gggcgccact tggacaccgt gcaacccccg aagtcgccgc cccgccctga 1500
gatcgccgcg acctggatcg tccacgcagc cagcgcagac cgccattgcg cgtgcgctcc 1560
ccgctgcgac gtcccgcgcg aacgtccttc cgcgcccgcc ggcccgcccg atgacgaggc 1620
gctcatcccg ccgtggctgt tcgccgagcg ccgtgccctc cgctgccgcg agtgggactt 1680
tgaggctctc cgcgcgcgcg ccgatacggc ggccgcgccc gccccgctgg ccccacgccc 1740
tgcgcggtac cccactgtgc tctaccgcca ccccgcccac cacggcccgt ggctcaccct 1800
tgacgagccg ggcgaggctg acgcggccct ggtcttatgc gacccgcttg gccagccgct 1860
ccggggccct gaacgccact tcgccgccgg cgcgcacatg tgcgcgcagg cgcgcgggct 1920
ccaggctttt gtccgtgttg tgcctccacc cgaacgcccc tgggccgacg ggggcgccag 1980
agcgtgggca aaattcttcc gcggctgcgc ctgggcgcag cgcttgctcg gcgagccggc 2040
agtgatgcac ctcccataca ccgatggtga cgtgccacag ctgatcgcgc tggccttgcg 2100
cacgctggcc cagcaggggg ccgccttggc actctcggtg cgagacctgc ccgggggtgc 2160
agcgttcgac gcgaatgcgg ttaccgccgc agtgcgcgct ggccccggcc agtccgcggc 2220
cacgtcaccg ccacccggcg accccccgcc accgcgccgc gcacggcgat cgcaacggca 2280
ctcggacgcc cgcagcactc cgccctccgc gcctgcgcgc gacccgccgc cgcccgcccc 2340
cagcccgccc gcgccacccc gcgcgggtga cctggccccc cccattctcg cggggccggc 2400
ggatcgcgcg cgtgacgccg agctggaggt cgcctgcgaa ccgagcggcc cccccgcgcc 2460
aaccaaggca gacccagaca gcgacatcgt tgaaagttac gcccgcgccg ccgggcccgt 2520
gcacctccgg gtccgtgaca tcatggaccc accgcctggg tgcaaggtcg tggtcaacgc 2580
cgccaacgag gggctgctgg ccggctctgg cgtgtgtggt gccatctttg ccaacgccac 2640
ggcggccctt gctgcagact gccggcgcct cgccccatgc cccaccggcg aggcagtggc 2700
gacacccggc cacggctgcg ggtacaccca catcatccac gccgttgccc cgcggcgccc 2760
tcgggacccc gccgccctcg aggagggcga ggcactgctc gagcgcgcct accgcagcat 2820
cgtcgcgcta gctgctgcgc gtaggtggac gtgtatcgcg tgccccctcc tcggcgctgg 2880
cgtctacggc tggtctgctg cggagtccct ccgcgccgcg cttgcggcca cgcgcgccga 2940
gcccgccgag cgcgtgagcc tgcacatctg ccaccccgac cgcgccacgc tgacgcacgc 3000
ctccgtgctc gttggcgcgg ggctcgctgc caggcgcgtc agcccccctc cggccgagcc 3060
cctcgcatct tgccccgccg gcgacccggg ccggccggct cagcgcagcg cgtcgccccc 3120
agcgaaaccc cttggggatg ccgccgcgcc cgagccccgc ggatgccagg ggtgcgaact 3180
ctgccggtac acgcgcgtca ctaatgaccg cgcctatgtc aacctgtggc tcgagcgcga 3240
ccgcggcgcc accagctggg cgatgcgcat tcccgaggtg gttgtctacg ggccggagca 3300
cctcgccacg cattttccat tgaaccacta cagtgtgctc aagcccgcgg aggtcaggcc 3360
cccgcgaggc atgtgcggga gtgacatgtg gcgctgccgc ggctggcagg gcatgccgca 3420
ggtgcggtgc accccctcca acgcccacgc cgccctgtgc cgcacaggcg taccccctcg 3480
agtgagcacg cgaggcggcg agctagaccc aaacacctgc tggctccgcg ccgccgccaa 3540
cgttgcgcag gctgcgcgcg cctgcggcgc ctacacgagc gccgggtgcc ccaagtgcgc 3600
ctacggccgc gccttgagcg aagcccgcac tcatgaggac tttgccgccc tgagccagcg 3660
gtggagcgcg agccacgccg atgcctcccc tgacggcacc ggagatcccc tcgaccccct 3720
gatggagacc gtgggatgcg cctgttcgcg cgtgtgggtc ggctccgagc acgaggcccc 3780
gcccgaccac ctcctggtgt ccctccaccg cgcccccaat ggtccgtggg gcgtagtgct 3840
cgaggtgcgc gcgcgccccg aagggggcaa ccccaccggc cacttcgttt gcgcggtcgg 3900
cggcggccca cgccgcgtct cggaccgccc ccatctttgg ctcgcggtcc ccctgtctcg 3960
gggcggcggc acctgcgccg tgaccgacga ggggctggcc caggcgtact acgacgatct 4020
cgaggtgcgc cgcctcgggg atgacgccat ggcccgggcg gccctcgcat cagtccaacg 4080
ccctcgcaaa ggcccctaca atattagggt atggaacatg gccgcaggcg ctggcaagac 4140
tacccgcatc ctcgctgcct tcacgcgcga agacctctac gtctgcccca ccaatgcgct 4200
cctgcacgag attcaggcca aactccgcgc gcgcgacatc gacatcaaga acgccgccac 4260
ctacgagcgc gcgctcacga aaccgctcgc cgcctaccgc cgcatctaca ttgatgaggc 4320
gttcaccctc ggcggcgagt actgcgcgtt cgttgccagc caaaccacag cggaggtgat 4380
ctgcgtcggt gaccgagacc agtgcggccc acactacgcc aacaactgcc gcacccctgt 4440
ccccgaccgc tggcctaccg agcgctcacg ccacacttgg cgcttccccg actgctgggc 4500
cgcccgcctg cgcgcggggc tcgattatga catcgagggc gagcgcatcg gcaccttcgc 4560
ctgcaacctt tgggacggcc gccaggtcga ccttcacctc gccttctcgc gcgaaaccgt 4620
gcgccgcctt cacgaggctg gcatacgcgc atacaccgtg cgcgaggccc agggcatgag 4680
cgtcggcacc gcctgcatcc atgtaggcag ggacggcacg gacgttgccc tggcgctgac 4740
acgcgacctt gccatcgtca gcctgacccg ggcctccgac gcgctctacc tccacgagct 4800
cgaggacggc tcactgcgcg ctgcggggct cagcgcgttc ctcgacgccg gggcactggc 4860
ggagctcaag gaggttcccg ctggcatcga ccgcgttgtc gccgtcgaac aggcaccacc 4920
accgttgccg cccgccgacg gcatccccga ggcccaagac gtgccaccct tctgtccccg 4980
cactctggag gagctcgtct tcggccgtgc cggccacccc cattacgcgg acctcaaccg 5040
cgtgaccgag ggcgaacggg aagtgcggta tatgcgcatc tcgcgccacc tgctcaacaa 5100
gaatcatacc gagatgcctg ggacggaacg cgtcctcagt gccgtttgcg ccgtgcggcg 5160
ctaccgcgcg ggcgaggatg ggtcgaccct ccgcactgct gtggcccgcc agcacccgcg 5220
ccctttccgc cagatcccac ccccgcgcgt cactgctggg gtcgcccagg agtggcgcat 5280
gacatacctg cgggaacgga tcgacctcac tgatgtctac acgcagatgg gcgtggctgc 5340
gcgggagctc accgaccgct acgcgcgccg ctaccctgag atcttcgccg gcatgtgtac 5400
cgcccagagc ctgagcgtcc ccgccttcct caaagccacc ctgaagtgcg tagacgccgc 5460
cctcggcccc agcgacaccg aggactgtca cgccgcccag gggaaagccg gccttgaaat 5520
ccgggcgtgg gccaaggagt gggttcaggt catgtccccg catttccgtg ctatccagaa 5580
gatcatcatg cgcgccttgc gcccgcaatt ccttgtggcc gcaggccata cggagcccga 5640
ggtcgatgcg tggtggcagg ctcactacac caccaacgcc atcgaggtcg acttcaccga 5700
gttcgacatg aaccagaccc tcgctactcg ggacgtcgag ctcgagatta gcgccgctct 5760
cttgggcctc ccctgcgccg aagactaccg tgcactccgc gctggcagct actgcaccct 5820
gcgcgaactg ggtaccactg agaccggctg cgagcgcaca agcggcgagc ccgccacgct 5880
gctgcacaac accaccgtgg ccatgtgcat ggctatgcgc atggtcccca aaggcgtgcg 5940
ctgggccggg attttccagg gcgacgacat ggtcatcttc ctccctgagg gcgcgcgcag 6000
cgcggcactt aagtggaccc ccgccgaggt gggcttgttt ggcttccaca tcccggtgaa 6060
gcatgtgagc acccctaccc ccagtttctg cgggcacgtc ggcaccgcgg ccggcctctt 6120
ccatgatgtc atgcaccagg cgatcaaggt gctctgccgc cgtttcgacc cagacgtgct 6180
tgaagaacag caggtggccc tcctcgaccg cctccggggg gtctacgcgg ccctgcctga 6240
caccgttgcc gccaatgccg cgtactacga ctacagcgcc gagcgcgtcc tcgccattgt 6300
gcgcgaactt accgcgtacg cgcgggggcg cggcctcgac cacccggcca ccatcggcgc 6360
gctcgaggag atccagaccc cctacgcgcg cgccaatctc cacgacgccg actaacgccc 6420
ctgtacgtgg ggcctttaat cttacctact ctaaccaggt catcacccac cgttgtttcg 6480
ccgcatctgg tgggtacccc actcttgcca ttcgggagag ccccagggtg cccgaatggg 6540
atccatggtc ttcacactcg aagatttcgt tggggactgg cgacagacag ccggctacaa 6600
cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc tcggggtgtc 6660
cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca 6720
tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt 6780
taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact atggcacact 6840
ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt atgaaggcat 6900
cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg gcaacaaaat 6960
tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa ccatcaacgg 7020
agtgaccggc tggcggctgt gcgaacgcat tctggcgtaa tctagacgcc cctctccccc 7080
ccccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg 7140
gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 7200
ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 7260
aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 7320
acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 7380
cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 7440
ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 7500
caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 7560
gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca 7620
cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga 7680
cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 7740
cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 7800
gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 7860
gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 7920
acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 7980
ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 8040
cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 8100
ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 8160
gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 8220
gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 8280
ggtgcctgaa tcgatagtca gctgcggggg cgagagcgat cgcgcgagcg cgcgggttat 8340
tgaccccgcc gcgcaatcgt tcaccggcgt ggtgtatggc acacacacca ctgctgtgtc 8400
ggagacccgg cagacctggg cggagtgggc tgccgcccat tggtggcagc tcactctggg 8460
cgctatttgc accctcctac tcgctggctt actcgcttgc tgtgccaaat gcttgtacta 8520
cttgcgcggc gctatagcgc cgcgctagtg ggcccccgcg cgaaacccgc attagcccac 8580
tggattcccg cacctgttgc tgcatagaaa aaaaaaaaaa aaaaaagaat tcgtaatcat 8640
ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 8700
ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 8760
cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 8820
tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 8880
ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 8940
taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 9000
agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 9060
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 9120
tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 9180
tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 9240
gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 9300
acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 9360
acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 9420
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 9480
gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 9540
gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 9600
agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 9660
ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 9720
ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 9780
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 9840
tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 9900
gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 9960
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 10020
caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 10080
cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 10140
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 10200
cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 10260
agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 10320
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 10380
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 10440
atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 10500
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 10560
cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 10620
caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 10680
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 10740
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 10800
aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 10860
gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 10920
tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 10980
gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 11040
tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 11100
gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 11160
tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 11220
ggttttccca gtcacgacgt tgtaaaacga cggccagtgc c 11261
<210> 72
<211> 12287
<212> DNA
<213> Artificial Sequence
<220>
<223> P150-717pmag-nMaghigh
<400> 72
actagtattt aggtgacact atagcaatgg gagctaacgg acctcgctta ggactcctat 60
tcccatggag aaactcctag atgaggttct tgcccccggt gggccttata acttaaccgt 120
cggcagttgg gtaagagacc atgtccgctc aattgtcgag ggcgcgtggg aagtgcgcga 180
tgtcgtcacc gctgcccaaa agcgggccat cgtagctgtg atacccagac ctgtattcac 240
gcagatgcag gttagtgatc acccagcact ccacgcaatt tcgcggtata cccgccgcca 300
ttggatcgag tggggcccta aagaagccct acacgtcctc atcgacccga gcccgggcct 360
gctccgcgag gtcgctcgcg ttgagcgccg ctgggtcgca ctgtgcctcc acaggacggc 420
acgcaaactc gccaccgccc tggccgagac ggccggcgag gcgtggcatg ccgactacgt 480
gtgcgcgctg cgtggcgcac cgagcggccc cttctacgtc caccctgagg acgtcccgca 540
cggcggccgc gccgtggcgg acagatgctt gctctactac acacccatgc agatgtgcga 600
gctgatgcgt gccattgacg ccaccttgct cgtggcggtt gacttgtggc cggtcgccct 660
tgcggctcat gtcggcgacg actgggacga cctgggcatc gcctggcacc tcgaccatga 720
cggcggctgc cccgccgact gccgcggagc cggcgctggg cccacgcccg gctacacccg 780
cccctgcacc acacgcatct accaagtcct gccggacacc gcccaccccg ggcgcctcta 840
ccggtgcggg ccccgcctgt ggacgcgcga ttgcgccgtg gccgaactct catgggaggt 900
tgcccagcac tgcgggcacc aggcgcgcgt gcgcgccgtg cgatgcaccc tccccatccg 960
ccacgtgcgc agcctccaac ccagcgcacg ggtccgactc ccggacctcg tccatctcgc 1020
cgaggtgggc cggtggcggt ggttcagcct cccccgcccc gtgttccagc gcatgctgtc 1080
ctactgcaag accctgagcc ccgacgcgta ctacagcgag cgtgtgttca agttcaagaa 1140
cgccctgagc cacagcatca cgctcgcggg caatgtgctg caagaggggt ggaagggcac 1200
gtgcgccgag gaagacgcgc tgtgcgcata cgtagccttc cgcgcgtggc agtctaacgc 1260
caggttggcg gggatcatga aaggcgctaa gcgctgcgct gccgactctt tgagcgtggc 1320
cggctggctg gacaccattt gggacgccat caagcggttc ttcggcagcg tgcccctcgc 1380
cgagcgcatg gaggagtggg aacaggacgc cgcggtcgcc gccttcgacc gcggccccct 1440
tgaggacggc gggcgccact tggacaccgt gcaacccccg aagtcgccgc cccgccctga 1500
gatcgccgcg acctggatcg tccacgcagc cagcgcagac cgccattgcg cgtgcgctcc 1560
ccgctgcgac gtcccgcgcg aacgtccttc cgcgcccgcc ggcccgcccg atgacgaggc 1620
gctcatcccg ccgtggctgt tcgccgagcg ccgtgccctc cgctgccgcg agtgggactt 1680
tgaggctctc cgcgcgcgcg ccgatacggc ggccgcgccc gccccgctgg ccccacgccc 1740
tgcgcggtac cccactgtgc tctaccgcca ccccgcccac cacggcccgt ggctcaccct 1800
tgacgagccg ggcgaggctg acgcggccct ggtcttatgc gacccgcttg gccagccgct 1860
ccggggccct gaacgccact tcgccgccgg cgcgcacatg tgcgcgcagg cgcgcgggct 1920
ccaggctttt gtccgtgttg tgcctccacc cgaacgcccc tgggccgacg ggggcgccag 1980
agcgtgggca aaattcttcc gcggctgcgc ctgggcgcag cgcttgctcg gcgagccggc 2040
agtgatgcac ctcccataca ccgatggtga cgtgccacag ctgatcgcgc tggccttgcg 2100
cacgctggcc cagcaggggg ccgccttggc actctcggtg cgagacctgc ccgggggtgc 2160
agcgttcgac gcgaatgcgg ttaccgccgc agtgcgcgct ggccccggcc agtccaagct 2220
tagccatact ctttatgccc ccggtggata tgacattatg ggatatctga ggcagatcag 2280
gaaccggcca aacccgcagg tggaactggg ccccgtggat acatcctgcg ccttgattct 2340
ttgtgacctg aaacagaaag acaccccgat agtttacgcg agtgaagcct tcctctacat 2400
gacaggttac agcaacgcag aggtgctggg ccggaattgc cggtttctgc aaagccctga 2460
cggcatggtg aagcccaaga gcacccggaa gtacgtggat agtaacacaa tcaatactat 2520
gcgcaaggca atcgacagga atgccgaggt gcaggttgaa gtagtcaatt ttaaaaagaa 2580
tggacagcga tttgttaatt tcctgactat gatacctgtt agggacgaaa caggcgagta 2640
tcgatactct atgggattcc agtgcgaaac agaaaccggt aattccgctg acggcggcgg 2700
aggatcgggt ggtagtggtg gttcaggagg aggatcgacc caaggaggat ccatgcatac 2760
tctttatgcc cccggtggat atgacattat gggatatctg gaccagatcg gcaaccggcc 2820
aaacccgcag gtggaactgg gccccgtgga tacatcctgc gccttgattc tttgtgacct 2880
gaaacagaaa gacaccccga tagtttacgc gagtgaagcc ttcctctaca tgacaggtta 2940
cagcaacgca gaggtgctgg gccggaattg ccggtttctg caaagccctg acggcatggt 3000
gaagcccaag agcacccgga agtacgtgga tagtaacaca atcaatacta tccgcaaggc 3060
aatcgacagg aatgccgagg tgcaggttga agtagtcaat tttaaaaaga atggacagcg 3120
atttgttaat ttcctgacta tcatacctgt tagggacgaa acaggcgagt atcgatactc 3180
tatgggattc cagtgcgaaa cagaatatcc gtacgacgtc ccagattacg cgggcaagct 3240
tgcggccacg tcaccgccac ccggcgaccc cccgccaccg cgccgcgcac ggcgatcgca 3300
acggcactcg gacgcccgca gcactccgcc ctccgcgcct gcgcgcgacc cgccgccgcc 3360
cgcccccagc ccgcccgcgc caccccgcgc gggtgacctg gcccccccca ttctcgcggg 3420
gccggcggat cgcgcgcgtg acgccgagct ggaggtcgcc tgcgaaccga gcggcccccc 3480
cgcgccaacc aaggcagacc cagacagcga catcgttgaa agttacgccc gcgccgccgg 3540
gcccgtgcac ctccgggtcc gtgacatcat ggacccaccg cctgggtgca aggtcgtggt 3600
caacgccgcc aacgaggggc tgctggccgg ctttggcgtg tgtggtgcca tctttgccaa 3660
cgccacggcg gcccttgctg cagactgccg gcgcctcgcc ccatgcccca ccggcgaggc 3720
agtggcgaca cccggccacg gctgcgggta cacccacatc atccacgccg ttgccccgcg 3780
gcgccctcgg gaccccgccg ccctcgagga gggcgaggca ctgctcgagc gcgcctaccg 3840
cagcatcgtc gcgctagctg ctgcgcgtag gtggacgtgt atcgcgtgcc ccctcctcgg 3900
cgctggcgtc tacggctggt ctgctgcgga gtccctccgc gccgcgcttg cggccacgcg 3960
cgccgagccc gccgagcgcg tgagcctgca catctgccac cccgaccgcg ccacgctgac 4020
gcacgcctcc gtgctcgttg gcgcggggct cgctgccagg cgcgtcagcc cccctccggc 4080
cgagcccctc gcatcttgcc ccgccggcga cccgggccgg ccggctcagc gcagcgcgtc 4140
gcccccagcg aaaccccttg gggatgccgc cgcgcccgag ccccgcggat gccaggggtg 4200
cgaactctgc cggtacacgc gcgtcactaa tgaccgcgcc tatgtcaacc tgtggctcga 4260
gcgcgaccgc ggcgccacca gctgggcgat gcgcattccc gaggtggttg tctacgggcc 4320
ggagcacctc gccacgcatt ttccattgaa ccactacagt gtgctcaagc ccgcggaggt 4380
caggcccccg cgaggcatgt gcgggagtga catgtggcgc tgccgcggct ggcagggcat 4440
gccgcaggtg cggtgcaccc cctccaacgc ccacgccgcc ctgtgccgca caggcgtacc 4500
ccctcgagtg agcacgcgag gcggcgagct agacccaaac acctgctggc tccgcgccgc 4560
cgccaacgtt gcgcaggctg cgcgcgcctg cggcgcctac acgagcgccg ggtgccccaa 4620
gtgcgcctac ggccgcgcct tgagcgaagc ccgcactcat gaggactttg ccgccctgag 4680
ccagcggtgg agcgcgagcc acgccgatgc ctcccctgac ggcaccggag atcccctcga 4740
ccccctgatg gagaccgtgg gatgcgcctg ttcgcgcgtg tgggtcggct ccgagcacga 4800
ggccccgccc gaccacctcc tggtgtccct ccaccgcgcc cccaatggtc cgtggggcgt 4860
agtgctcgag gtgcgcgcgc gccccgaagg gggcaacccc accggccact tcgtttgcgc 4920
ggtcggcggc ggcccacgcc gcgtctcgga ccgcccccat ctttggctcg cggtccccct 4980
gtctcggggc ggcggcacct gcgccgtgac cgacgagggg ctggcccagg cgtactacga 5040
cgatctcgag gtgcgccgcc tcggggatga cgccatggcc cgggcggccc tcgcatcagt 5100
ccaacgccct cgcaaaggcc cctacaatat tagggtatgg aacatggccg caggcgctgg 5160
caagactacc cgcatcctcg ctgccttcac gcgcgaagac ctctacgtct gccccaccaa 5220
tgcgctcctg cacgagattc aggccaaact ccgcgcgcgc gacatcgaca tcaagaacgc 5280
cgccacctac gagcgcgcgc tcacgaaacc gctcgccgcc taccgccgca tctacattga 5340
tgaggcgttc accctcggcg gcgagtactg cgcgttcgtt gccagccaaa ccacagcgga 5400
ggtgatctgc gtcggtgacc gagaccagtg cggcccacac tacgccaaca actgccgcac 5460
ccctgtcccc gaccgctggc ctaccgagcg ctcacgccac acttggcgct tccccgactg 5520
ctgggccgcc cgcctgcgcg cggggctcga ttatgacatc gagggcgagc gcatcggcac 5580
cttcgcctgc aacctttggg acggccgcca ggtcgacctt cacctcgcct tctcgcgcga 5640
aaccgtgcgc cgccttcacg aggctggcat acgcgcatac accgtgcgcg aggcccaggg 5700
catgagcgtc ggcaccgcct gcatccatgt aggcagggac ggcacggacg ttgccctggc 5760
gctgacacgc gaccttgcca tcgtcagcct gacccgggcc tccgacgcgc tctacctcca 5820
cgagctcgag gacggctcac tgcgcgctgc ggggctcagc gcgttcctcg acgccggggc 5880
actggcggag ctcaaggagg ttcccgctgg catcgaccgc gttgtcgccg tcgaacaggc 5940
accaccaccg ttgccgcccg ccgacggcat ccccgaggcc caagacgtgc cacccttctg 6000
tccccgcact ctggaggagc tcgtcttcgg ccgtgccggc cacccccatt acgcggacct 6060
caaccgcgtg accgagggcg aacgggaagt gcggtatatg cgcatctcgc gccacctgct 6120
caacaagaat cataccgaga tgcctgggac ggaacgcgtc ctcagtgccg tttgcgccgt 6180
gcggcgctac cgcgcgggcg aggatgggtc gaccctccgc actgctgtgg cccgccagca 6240
cccgcgccct ttccgccaga tcccaccccc gcgcgtcact gctggggtcg cccaggagtg 6300
gcgcatgaca tacctgcggg aacggatcga cctcactgat gtctacacgc agatgggcgt 6360
ggctgcgcgg gagctcaccg accgctacgc gcgccgctac cctgagatct tcgccggcat 6420
gtgtaccgcc cagagcctga gcgtccccgc cttcctcaaa gccaccctga agtgcgtaga 6480
cgccgccctc ggccccagcg acaccgagga ctgtcacgcc gcccagggga aagccggcct 6540
tgaaatccgg gcgtgggcca aggagtgggt tcaggtcatg tccccgcatt tccgtgctat 6600
ccagaagatc atcatgcgcg ccttgcgccc gcaattcctt gtggccgcag gccatacgga 6660
gcccgaggtc gatgcgtggt ggcaggctca ctacaccacc aacgccatcg aggtcgactt 6720
caccgagttc gacatgaacc agaccctcgc tactcgggac gtcgagctcg agattagcgc 6780
cgctctcttg ggcctcccct gcgccgaaga ctaccgtgca ctccgcgctg gcagctactg 6840
caccctgcgc gaactgggta ccactgagac cggctgcgag cgcacaagcg gcgagcccgc 6900
cacgctgctg cacaacacca ccgtggccat gtgcatggct atgcgcatgg tccccaaagg 6960
cgtgcgctgg gccgggattt tccagggcga cgacatggtc atcttcctcc ctgagggcgc 7020
gcgcagcgcg gcacttaagt ggacccccgc cgaggtgggc ttgtttggct tccacatccc 7080
ggtgaagcat gtgagcaccc ctacccccag tttctgcggg cacgtcggca ccgcggccgg 7140
cctcttccat gatgtcatgc accaggcgat caaggtgctc tgccgccgtt tcgacccaga 7200
cgtgcttgaa gaacagcagg tggccctcct cgaccgcctc cggggggtct acgcggccct 7260
gcctgacacc gttgccgcca atgccgcgta ctacgactac agcgccgagc gcgtcctcgc 7320
cattgtgcgc gaacttaccg cgtacgcgcg ggggcgcggc ctcgaccacc cggccaccat 7380
cggcgcgctc gaggagatcc agacccccta cgcgcgcgcc aatctccacg acgccgacta 7440
acgcccctgt acgtggggcc tttaatctta cctactctaa ccaggtcatc acccaccgtt 7500
gtttcgccgc atctggtggg taccccactc ttgccattcg ggagagcccc agggtgcccg 7560
aatgggatcc atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg 7620
ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg 7680
ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga 7740
catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa 7800
aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg 7860
cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga 7920
aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa 7980
caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat 8040
caacggagtg accggctggc ggctgtgcga acgcattctg gcgtaatcta gacgcccctc 8100
tccccccccc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata 8160
aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt 8220
gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct 8280
cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 8340
ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga 8400
caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 8460
ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 8520
attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 8580
gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc 8640
gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca 8700
aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 8760
gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 8820
accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 8880
cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 8940
tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 9000
gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 9060
caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 9120
ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 9180
gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 9240
ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 9300
aagcccggtg cctgaatcga tagtcagctg cgggggcgag agcgatcgcg cgagcgcgcg 9360
ggttattgac cccgccgcgc aatcgttcac cggcgtggtg tatggcacac acaccactgc 9420
tgtgtcggag acccggcaga cctgggcgga gtgggctgcc gcccattggt ggcagctcac 9480
tctgggcgct atttgcaccc tcctactcgc tggcttactc gcttgctgtg ccaaatgctt 9540
gtactacttg cgcggcgcta tagcgccgcg ctagtgggcc cccgcgcgaa acccgcatta 9600
gcccactgga ttcccgcacc tgttgctgca tagaaaaaaa aaaaaaaaaa aagaattcgt 9660
aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 9720
tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 9780
taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 9840
aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 9900
cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 9960
aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 10020
aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 10080
tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 10140
caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 10200
cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 10260
ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 10320
gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 10380
agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 10440
gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 10500
acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 10560
gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 10620
gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 10680
cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 10740
caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 10800
gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 10860
cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 10920
cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 10980
caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 11040
gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 11100
gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 11160
cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 11220
catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 11280
gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 11340
ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 11400
gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 11460
cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 11520
tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 11580
gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 11640
atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 11700
ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 11760
gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 11820
acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 11880
cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 11940
agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 12000
cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 12060
tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 12120
tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 12180
cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 12240
cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgcc 12287
<210> 73
<211> 12287
<212> DNA
<213> Artificial Sequence
<220>
<223> P150-1005pmag-nMaghigh
<400> 73
actagtattt aggtgacact atagcaatgg gagctaacgg acctcgctta ggactcctat 60
tcccatggag aaactcctag atgaggttct tgcccccggt gggccttata acttaaccgt 120
cggcagttgg gtaagagacc atgtccgctc aattgtcgag ggcgcgtggg aagtgcgcga 180
tgtcgtcacc gctgcccaaa agcgggccat cgtagctgtg atacccagac ctgtattcac 240
gcagatgcag gttagtgatc acccagcact ccacgcaatt tcgcggtata cccgccgcca 300
ttggatcgag tggggcccta aagaagccct acacgtcctc atcgacccga gcccgggcct 360
gctccgcgag gtcgctcgcg ttgagcgccg ctgggtcgca ctgtgcctcc acaggacggc 420
acgcaaactc gccaccgccc tggccgagac ggccggcgag gcgtggcatg ccgactacgt 480
gtgcgcgctg cgtggcgcac cgagcggccc cttctacgtc caccctgagg acgtcccgca 540
cggcggccgc gccgtggcgg acagatgctt gctctactac acacccatgc agatgtgcga 600
gctgatgcgt gccattgacg ccaccttgct cgtggcggtt gacttgtggc cggtcgccct 660
tgcggctcat gtcggcgacg actgggacga cctgggcatc gcctggcacc tcgaccatga 720
cggcggctgc cccgccgact gccgcggagc cggcgctggg cccacgcccg gctacacccg 780
cccctgcacc acacgcatct accaagtcct gccggacacc gcccaccccg ggcgcctcta 840
ccggtgcggg ccccgcctgt ggacgcgcga ttgcgccgtg gccgaactct catgggaggt 900
tgcccagcac tgcgggcacc aggcgcgcgt gcgcgccgtg cgatgcaccc tccccatccg 960
ccacgtgcgc agcctccaac ccagcgcacg ggtccgactc ccggacctcg tccatctcgc 1020
cgaggtgggc cggtggcggt ggttcagcct cccccgcccc gtgttccagc gcatgctgtc 1080
ctactgcaag accctgagcc ccgacgcgta ctacagcgag cgtgtgttca agttcaagaa 1140
cgccctgagc cacagcatca cgctcgcggg caatgtgctg caagaggggt ggaagggcac 1200
gtgcgccgag gaagacgcgc tgtgcgcata cgtagccttc cgcgcgtggc agtctaacgc 1260
caggttggcg gggatcatga aaggcgctaa gcgctgcgct gccgactctt tgagcgtggc 1320
cggctggctg gacaccattt gggacgccat caagcggttc ttcggcagcg tgcccctcgc 1380
cgagcgcatg gaggagtggg aacaggacgc cgcggtcgcc gccttcgacc gcggccccct 1440
tgaggacggc gggcgccact tggacaccgt gcaacccccg aagtcgccgc cccgccctga 1500
gatcgccgcg acctggatcg tccacgcagc cagcgcagac cgccattgcg cgtgcgctcc 1560
ccgctgcgac gtcccgcgcg aacgtccttc cgcgcccgcc ggcccgcccg atgacgaggc 1620
gctcatcccg ccgtggctgt tcgccgagcg ccgtgccctc cgctgccgcg agtgggactt 1680
tgaggctctc cgcgcgcgcg ccgatacggc ggccgcgccc gccccgctgg ccccacgccc 1740
tgcgcggtac cccactgtgc tctaccgcca ccccgcccac cacggcccgt ggctcaccct 1800
tgacgagccg ggcgaggctg acgcggccct ggtcttatgc gacccgcttg gccagccgct 1860
ccggggccct gaacgccact tcgccgccgg cgcgcacatg tgcgcgcagg cgcgcgggct 1920
ccaggctttt gtccgtgttg tgcctccacc cgaacgcccc tgggccgacg ggggcgccag 1980
agcgtgggca aaattcttcc gcggctgcgc ctgggcgcag cgcttgctcg gcgagccggc 2040
agtgatgcac ctcccataca ccgatggtga cgtgccacag ctgatcgcgc tggccttgcg 2100
cacgctggcc cagcaggggg ccgccttggc actctcggtg cgagacctgc ccgggggtgc 2160
agcgttcgac gcgaatgcgg ttaccgccgc agtgcgcgct ggccccggcc agtccgcggc 2220
cacgtcaccg ccacccggcg accccccgcc accgcgccgc gcacggcgat cgcaacggca 2280
ctcggacgcc cgcagcactc cgccctccgc gcctgcgcgc gacccgccgc cgcccgcccc 2340
cagcccgccc gcgccacccc gcgcgggtga cctggccccc cccattctcg cggggccggc 2400
ggatcgcgcg cgtgacgccg agctggaggt cgcctgcgaa ccgagcggcc cccccgcgcc 2460
aaccaaggca gacccagaca gcgacatcgt tgaaagttac gcccgcgccg ccgggcccgt 2520
gcacctccgg gtccgtgaca tcatggaccc accgcctggg tgcaaggtcg tggtcaacgc 2580
cgccaacgag gggctgctgg ccggctttgg cgtgtgtggt gccatctttg ccaacgccac 2640
ggcggccctt gctgcagact gccggcgcct cgccccatgc cccaccggcg aggcagtggc 2700
gacacccggc cacggctgcg ggtacaccca catcatccac gccgttgccc cgcggcgccc 2760
tcgggacccc gccgccctcg aggagggcga ggcactgctc gagcgcgcct accgcagcat 2820
cgtcgcgcta gctgctgcgc gtaggtggac gtgtatcgcg tgccccctcc tcggcgctgg 2880
cgtctacggc tggtctgctg cggagtccct ccgcgccgcg cttgcggcca cgcgcgccga 2940
gcccgccgag cgcgtgagcc tgcacatctg ccaccccgac cgcgccacgc tgacgcacgc 3000
ctccgtgctc gttggcgcgg ggctcgctgc caggcgcgtc agcccccctc cggccgagcc 3060
cctcgcatct tgccccgcca agcttagcca tactctttat gcccccggtg gatatgacat 3120
tatgggatat ctgaggcaga tcaggaaccg gccaaacccg caggtggaac tgggccccgt 3180
ggatacatcc tgcgccttga ttctttgtga cctgaaacag aaagacaccc cgatagttta 3240
cgcgagtgaa gccttcctct acatgacagg ttacagcaac gcagaggtgc tgggccggaa 3300
ttgccggttt ctgcaaagcc ctgacggcat ggtgaagccc aagagcaccc ggaagtacgt 3360
ggatagtaac acaatcaata ctatgcgcaa ggcaatcgac aggaatgccg aggtgcaggt 3420
tgaagtagtc aattttaaaa agaatggaca gcgatttgtt aatttcctga ctatgatacc 3480
tgttagggac gaaacaggcg agtatcgata ctctatggga ttccagtgcg aaacagaaac 3540
cggtaattcc gctgacggcg gcggaggatc gggtggtagt ggtggttcag gaggaggatc 3600
gacccaagga ggatccatgc atactcttta tgcccccggt ggatatgaca ttatgggata 3660
tctggaccag atcggcaacc ggccaaaccc gcaggtggaa ctgggccccg tggatacatc 3720
ctgcgccttg attctttgtg acctgaaaca gaaagacacc ccgatagttt acgcgagtga 3780
agccttcctc tacatgacag gttacagcaa cgcagaggtg ctgggccgga attgccggtt 3840
tctgcaaagc cctgacggca tggtgaagcc caagagcacc cggaagtacg tggatagtaa 3900
cacaatcaat actatccgca aggcaatcga caggaatgcc gaggtgcagg ttgaagtagt 3960
caattttaaa aagaatggac agcgatttgt taatttcctg actatcatac ctgttaggga 4020
cgaaacaggc gagtatcgat actctatggg attccagtgc gaaacagaat atccgtacga 4080
cgtcccagat tacgcgggca agcttggcga cccgggccgg ccggctcagc gcagcgcgtc 4140
gcccccagcg aaaccccttg gggatgccgc cgcgcccgag ccccgcggat gccaggggtg 4200
cgaactctgc cggtacacgc gcgtcactaa tgaccgcgcc tatgtcaacc tgtggctcga 4260
gcgcgaccgc ggcgccacca gctgggcgat gcgcattccc gaggtggttg tctacgggcc 4320
ggagcacctc gccacgcatt ttccattgaa ccactacagt gtgctcaagc ccgcggaggt 4380
caggcccccg cgaggcatgt gcgggagtga catgtggcgc tgccgcggct ggcagggcat 4440
gccgcaggtg cggtgcaccc cctccaacgc ccacgccgcc ctgtgccgca caggcgtacc 4500
ccctcgagtg agcacgcgag gcggcgagct agacccaaac acctgctggc tccgcgccgc 4560
cgccaacgtt gcgcaggctg cgcgcgcctg cggcgcctac acgagcgccg ggtgccccaa 4620
gtgcgcctac ggccgcgcct tgagcgaagc ccgcactcat gaggactttg ccgccctgag 4680
ccagcggtgg agcgcgagcc acgccgatgc ctcccctgac ggcaccggag atcccctcga 4740
ccccctgatg gagaccgtgg gatgcgcctg ttcgcgcgtg tgggtcggct ccgagcacga 4800
ggccccgccc gaccacctcc tggtgtccct ccaccgcgcc cccaatggtc cgtggggcgt 4860
agtgctcgag gtgcgcgcgc gccccgaagg gggcaacccc accggccact tcgtttgcgc 4920
ggtcggcggc ggcccacgcc gcgtctcgga ccgcccccat ctttggctcg cggtccccct 4980
gtctcggggc ggcggcacct gcgccgtgac cgacgagggg ctggcccagg cgtactacga 5040
cgatctcgag gtgcgccgcc tcggggatga cgccatggcc cgggcggccc tcgcatcagt 5100
ccaacgccct cgcaaaggcc cctacaatat tagggtatgg aacatggccg caggcgctgg 5160
caagactacc cgcatcctcg ctgccttcac gcgcgaagac ctctacgtct gccccaccaa 5220
tgcgctcctg cacgagattc aggccaaact ccgcgcgcgc gacatcgaca tcaagaacgc 5280
cgccacctac gagcgcgcgc tcacgaaacc gctcgccgcc taccgccgca tctacattga 5340
tgaggcgttc accctcggcg gcgagtactg cgcgttcgtt gccagccaaa ccacagcgga 5400
ggtgatctgc gtcggtgacc gagaccagtg cggcccacac tacgccaaca actgccgcac 5460
ccctgtcccc gaccgctggc ctaccgagcg ctcacgccac acttggcgct tccccgactg 5520
ctgggccgcc cgcctgcgcg cggggctcga ttatgacatc gagggcgagc gcatcggcac 5580
cttcgcctgc aacctttggg acggccgcca ggtcgacctt cacctcgcct tctcgcgcga 5640
aaccgtgcgc cgccttcacg aggctggcat acgcgcatac accgtgcgcg aggcccaggg 5700
catgagcgtc ggcaccgcct gcatccatgt aggcagggac ggcacggacg ttgccctggc 5760
gctgacacgc gaccttgcca tcgtcagcct gacccgggcc tccgacgcgc tctacctcca 5820
cgagctcgag gacggctcac tgcgcgctgc ggggctcagc gcgttcctcg acgccggggc 5880
actggcggag ctcaaggagg ttcccgctgg catcgaccgc gttgtcgccg tcgaacaggc 5940
accaccaccg ttgccgcccg ccgacggcat ccccgaggcc caagacgtgc cacccttctg 6000
tccccgcact ctggaggagc tcgtcttcgg ccgtgccggc cacccccatt acgcggacct 6060
caaccgcgtg accgagggcg aacgggaagt gcggtatatg cgcatctcgc gccacctgct 6120
caacaagaat cataccgaga tgcctgggac ggaacgcgtc ctcagtgccg tttgcgccgt 6180
gcggcgctac cgcgcgggcg aggatgggtc gaccctccgc actgctgtgg cccgccagca 6240
cccgcgccct ttccgccaga tcccaccccc gcgcgtcact gctggggtcg cccaggagtg 6300
gcgcatgaca tacctgcggg aacggatcga cctcactgat gtctacacgc agatgggcgt 6360
ggctgcgcgg gagctcaccg accgctacgc gcgccgctac cctgagatct tcgccggcat 6420
gtgtaccgcc cagagcctga gcgtccccgc cttcctcaaa gccaccctga agtgcgtaga 6480
cgccgccctc ggccccagcg acaccgagga ctgtcacgcc gcccagggga aagccggcct 6540
tgaaatccgg gcgtgggcca aggagtgggt tcaggtcatg tccccgcatt tccgtgctat 6600
ccagaagatc atcatgcgcg ccttgcgccc gcaattcctt gtggccgcag gccatacgga 6660
gcccgaggtc gatgcgtggt ggcaggctca ctacaccacc aacgccatcg aggtcgactt 6720
caccgagttc gacatgaacc agaccctcgc tactcgggac gtcgagctcg agattagcgc 6780
cgctctcttg ggcctcccct gcgccgaaga ctaccgtgca ctccgcgctg gcagctactg 6840
caccctgcgc gaactgggta ccactgagac cggctgcgag cgcacaagcg gcgagcccgc 6900
cacgctgctg cacaacacca ccgtggccat gtgcatggct atgcgcatgg tccccaaagg 6960
cgtgcgctgg gccgggattt tccagggcga cgacatggtc atcttcctcc ctgagggcgc 7020
gcgcagcgcg gcacttaagt ggacccccgc cgaggtgggc ttgtttggct tccacatccc 7080
ggtgaagcat gtgagcaccc ctacccccag tttctgcggg cacgtcggca ccgcggccgg 7140
cctcttccat gatgtcatgc accaggcgat caaggtgctc tgccgccgtt tcgacccaga 7200
cgtgcttgaa gaacagcagg tggccctcct cgaccgcctc cggggggtct acgcggccct 7260
gcctgacacc gttgccgcca atgccgcgta ctacgactac agcgccgagc gcgtcctcgc 7320
cattgtgcgc gaacttaccg cgtacgcgcg ggggcgcggc ctcgaccacc cggccaccat 7380
cggcgcgctc gaggagatcc agacccccta cgcgcgcgcc aatctccacg acgccgacta 7440
acgcccctgt acgtggggcc tttaatctta cctactctaa ccaggtcatc acccaccgtt 7500
gtttcgccgc atctggtggg taccccactc ttgccattcg ggagagcccc agggtgcccg 7560
aatgggatcc atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg 7620
ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg 7680
ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga 7740
catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa 7800
aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg 7860
cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga 7920
aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa 7980
caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat 8040
caacggagtg accggctggc ggctgtgcga acgcattctg gcgtaatcta gacgcccctc 8100
tccccccccc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata 8160
aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt 8220
gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct 8280
cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 8340
ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga 8400
caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 8460
ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 8520
attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 8580
gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc 8640
gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca 8700
aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 8760
gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 8820
accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 8880
cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 8940
tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 9000
gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 9060
caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 9120
ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 9180
gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 9240
ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 9300
aagcccggtg cctgaatcga tagtcagctg cgggggcgag agcgatcgcg cgagcgcgcg 9360
ggttattgac cccgccgcgc aatcgttcac cggcgtggtg tatggcacac acaccactgc 9420
tgtgtcggag acccggcaga cctgggcgga gtgggctgcc gcccattggt ggcagctcac 9480
tctgggcgct atttgcaccc tcctactcgc tggcttactc gcttgctgtg ccaaatgctt 9540
gtactacttg cgcggcgcta tagcgccgcg ctagtgggcc cccgcgcgaa acccgcatta 9600
gcccactgga ttcccgcacc tgttgctgca tagaaaaaaa aaaaaaaaaa aagaattcgt 9660
aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 9720
tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 9780
taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 9840
aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 9900
cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 9960
aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 10020
aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 10080
tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 10140
caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 10200
cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 10260
ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 10320
gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 10380
agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 10440
gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 10500
acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 10560
gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 10620
gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 10680
cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 10740
caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 10800
gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 10860
cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 10920
cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 10980
caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 11040
gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 11100
gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 11160
cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 11220
catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 11280
gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 11340
ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 11400
gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 11460
cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 11520
tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 11580
gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 11640
atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 11700
ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 11760
gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 11820
acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 11880
cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 11940
agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 12000
cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 12060
tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 12120
tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 12180
cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 12240
cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgcc 12287
<210> 74
<211> 16356
<212> DNA
<213> Artificial Sequence
<220>
<223> Ln-pmag 质粒
<400> 74
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagatg gccacacttt 120
tgaggagctt agcattgttc aaaagaaaca aggacaaacc acccattaca tcaggatccg 180
gtggagccat cagaggaatc aaacacatta ttatagtacc aattcctgga gattcctcaa 240
ttaccactcg atccagacta ctggaccggt tggtcaggtt aattggaaac ccggatgtga 300
gcgggcccaa actaacaggg gcactaatag gtatattatc cttatttgtg gagtctccag 360
gtcaattgat tcagaggatc accgatgacc ctgacgttag catcaggctg ttagaggttg 420
ttcagagtga ccagtcacaa tctggcctta ccttcgcatc aagaggtacc aacatggagg 480
atgaggcgga ccaatacttt tcacatgatg atccaagcag tagtgatcaa tccaggtccg 540
gatggttcga gaacaaggaa atctcagata ttgaagtgca agaccctgag ggattcaaca 600
tgattctggg taccattcta gcccagatct gggtcttgct cgcaaaggcg gttacggccc 660
cagacacggc agctgattcg gagctaagaa ggtggataaa gtacacccaa caaagaaggg 720
tagttggtga atttagattg gagagaaaat ggttggatgt ggtgaggaac aggattgccg 780
aggacctctc tttacgccga ttcatggtgg ctctaatcct ggatatcaag aggacacccg 840
ggaacaaacc taggattgct gaaatgatat gtgacattga tacatatatc gtagaggcag 900
gattagccag ttttatcctg actattaagt ttgggataga aactatgtat cctgctcttg 960
gactgcatga atttgctggt gagttatcca cacttgagtc cttgatgaat ctttaccagc 1020
aaatgggaga aactgcaccc tacatggtaa tcctagagaa ctcaattcag aacaagttca 1080
gtgcaggatc ataccctctg ctctggagct atgccatggg agtaggagtg gaacttgaaa 1140
actccatggg aggtttgaac tttggtcgat cttactttga tccagcatat tttagattag 1200
ggcaagagat ggtgaggagg tcagctggaa aggtcagttc cacattggca tccgaactcg 1260
gtatcactgc cgaggatgca aggcttgttt cagagattgc aatgcatact actgaggaca 1320
ggatcagtag agcggtcgga cccagacaag cccaagtgtc atttctacac ggtgatcaaa 1380
gtgagaatga gctaccagga ttggggggca aggaagatag gagggtcaaa cagggtcggg 1440
gagaagccag ggagagctac agagaaaccg ggtccagcag agcaagtgat gcgagagctg 1500
cccatcctcc aaccagcatg cccctagaca ttgacactgc atcggagtca ggccaagatc 1560
cgcaggacag tcgaaggtca gctgacgccc tgctcaggct gcaagccatg gcaggaatct 1620
tggaagaaca aggctcagac acggacaccc ctagggtata caatgacaga gatcttctag 1680
actaggtgcg agaggccgag gaccagaaca acatccgcct accctccatc attgttataa 1740
aaaacttagg aaccaggtcc acacagccgc cagccaacca accatccact cccacgactg 1800
gagccgatgg cagaagagca ggcacgccat gtcaaaaacg gactggaatg catccgggct 1860
ctcaaggccg agcccatcgg ctcactggcc gtcgaggaag ccatggcagc atggtcagaa 1920
atatcagaca acccaggaca ggaccgagcc acctgcaagg aagaggaggc aggcagttcg 1980
ggtctcagca aaccatgcct ctcagcaatt ggatcaactg aaggcggtgc acctcgcatc 2040
cgcggtcagg gatctggaga aagcgatgac gacgctgaaa ctttgggaat cccctcaaga 2100
aatctccagg catcaagcac tgggttacag tgttatcatg tttatgatca cagcggtgaa 2160
gcggttaagg gaatccaaga tgctgactct atcatggttc aatcaggcct tgatggtgat 2220
agcaccctct caggaggaga cgatgaatct gaaaacagcg atgtggatat tggcgaacct 2280
gataccgagg gatatgctat cactgaccgg ggatctgctc ccatctctat ggggttcagg 2340
gcttctgatg ttgaaactgc agaaggaggg gagatccacg agctcctgaa actccaatcc 2400
agaggcaaca actttccgaa gcttgggaaa actctcaatg ttcctccgcc cccgaacccc 2460
agtagggcca gcacttccga gacacccatt aaaaagggca cagacgcgag attggcctca 2520
tttggaacgg agatcgcgtc tttattgaca ggtggtgcaa cccaatgtgc tcgaaagtca 2580
ccctcggaac catcagggcc aggtgcacct gcggggaatg tccccgagtg tgtgagcaat 2640
gccgcactga tacaggagtg gacacccgaa tctggtacca caatctcccc gagatcccag 2700
aataatgaag aagggggaga ctattatgat gatgagctgt tctccgatgt ccaagacatc 2760
aaaacagcct tggccaaaat acacgaggat aatcagaaga taatctccaa gctagaatca 2820
ttgctgttat tgaagggaga agttgagtca attaagaagc agatcaacag gcaaaatatc 2880
agcatatcca ccctggaagg acacctctca agcatcatga ttgccattcc tggacttggg 2940
aaggatccca acgaccccac tgcagatgtc gaactcaatc ccgacctgaa acccatcata 3000
ggcagagatt caggccgagc actggccgaa gttctcaaga agcccgttgc cagccgacaa 3060
ctccagggaa tgactaatgg acggaccagt tccagaggac agctgctgaa ggaatttcaa 3120
ctaaagccga tcgggaaaaa ggtgagctca gccgtcgggt ttgttcctga caccggccct 3180
gcatcacgca gtgtaatccg ctccattata aaatccagcc ggctagagga ggatcggaag 3240
cgttacctga tgactctcct tgatgatatc aaaggagcca acgatcttgc caagttccac 3300
cagatgctga tgaagataat aatgaagtag ctacagctca acttacctgc caaccccatg 3360
ccagtcgacc taattagtac aacctaaatc cattataaaa aacttaggag caaagtgatt 3420
gcctcctaag ttccacaatg acagagatct acgatttcga caagtcggca tgggacatca 3480
aagggtcgat cgctccgata caacctacca cctacagtga tggcaggctg gtgccccagg 3540
tcagagtcat agatcctggt ctaggtgata ggaaggatga atgctttatg tacatgtttc 3600
tgctgggggt tgttgaggac agcgatcccc tagggcctcc aatcgggcga gcattcgggt 3660
ccctgccctt aggtgttggt agatccacag caaaacccga ggaactcctc aaagaggcca 3720
ctgagcttga catagttgtt agacgtacag cagggctcaa tgaaaaactg gtgttctaca 3780
acaacacccc actaaccctc ctcacacctt ggagaaaggt cctaacaaca gggagtgtct 3840
tcaatgcaaa ccaagtgtgc aatgcggtta atctaatacc gctggacacc ccgcagaggt 3900
tccgtgttgt ttatatgagc atcacccgtc tttcggataa cgggtattac accgttccca 3960
gaagaatgct ggaattcaga tcggtcaatg cagtggcctt caacctgcta gtgaccctta 4020
ggattgacaa ggcgattggc cctgggaaga tcatcgacaa tgcagagcaa cttcctgagg 4080
caacatttat ggtccacatc gggaacttca ggagaaagaa gagtgaagtc tactctgccg 4140
attattgcaa aatgaaaatc gaaaagatgg gcctggtttt tgcacttggt gggatagggg 4200
gcaccagtct tcacattaga agcacaggca aaatgagcaa gactctccat gcacaactcg 4260
ggttcaagaa gaccttatgt tacccactga tggatatcaa tgaagacctt aatcggttac 4320
tctggaggag cagatgcaag atagtaagaa tccaggcagt tttgcagcca tcagttcctc 4380
aagaattccg catttacgac gacgtgatca taaatgatga ccaaggacta ttcaaagttc 4440
tgtagaccgc agtgcccagc aatacccgaa aacgaccccc ctcataatga cagccagaag 4500
gcccggacaa aaaagccccc tccagaagac tccacggacc aagcgagagg ccagccagca 4560
gccgacagca agtgtggaca ccaggcggcc caagcacaga acagccccga cacaaggcca 4620
ccaccagcca tcccaatctg cgtcctcctc gtgggacccc cgaggaccaa ccccgaaggt 4680
cgctccgaac acagaccacc aaccgcatcc ccacagctcc cgggaaagga acccccagca 4740
actggaaggc ccctcccccc ctcccccaac gcaagaaccc cacaaccgaa ccgcacaagc 4800
gaccgaggtg acccaaccgc aggcatccga ctccttagac agatcctctc cccccggcat 4860
actaaacaaa acttagggcc aaggaacaca cacactcgac agaacccaga ccccggcccg 4920
cggcaccgcg cccccacccc ccgaaaacca gagggagccc ccaaccaaac ccgccggccc 4980
ccccggtgcc cacaggtagg cacaccaacc cccgaccaga cccagcaccc agccaccgac 5040
aatccaagac ggggggcccc ccccaaaaaa aggcccccag gggccgacag ccagcatcgc 5100
gaggaagcac acccacccca cacacgacca cggcaaccga accagagtcc agaccaccct 5160
gggccaccag ctcccagact cggccatcac cccgcaaaaa ggaaaggcca caacccgcgc 5220
accccagccc cgatccggcg ggcagccact caacccgaac cagcacccaa gagcgatccc 5280
tgggggaccc ccaaaccgca aaagacatca gtatcccaca gcctctccaa gtcccccggt 5340
ctcctcctct tctcgaaggg accaaaagat caatccacca catccgacga cactcaattc 5400
cccaccccca aaggagacac cgggaatccc agaatcaaga ctcatccagt gtccatcatg 5460
ggtctcaagg tgaacgtctc tgccatattc atggcagtac tgttaactct ccaaacaccc 5520
accggtcaaa tccattgggg caatctctct aagatagggg tggtagggat aggaagtgca 5580
agctacaaag ttatgactcg ttccagccat caatcattgg tcataaaatt aatgcccaat 5640
ataactctcc tcaataactg cacgagggta gagatcgcag aatacaggag actactgaga 5700
acagttttgg aaccaattag agatgcactt aatgcaatga cccagaatat aagaccggtt 5760
cagagtgtag cttcaagtag gagacacaag agatttgcgg gagttgtcct ggcaggtgcg 5820
gccctaggcg ttgccacagc tgctcagata acagccggca ttgcacttca ccagtccatg 5880
ctgaactctc aagccatcga caatctgaga gcaagcctgg aaactactaa tcaggcaatt 5940
gaggcaatca gacaagcagg gcaggagatg atattggctg ttcagggtgt ccaagactac 6000
atcaataatg agctgatacc gtctatgaac caactatctt gtgatttaat cggccagaag 6060
ctagggctca aattgctcag atactataca gaaatcctgt cattatttgg ccccagctta 6120
cgggacccca tatctgcgga gatatccatc caggctttga gctatgcgct tggaggagat 6180
atcaataagg tattagaaaa gctcggatac agtggaggtg atttactggg catcttagag 6240
agcagaggaa taaaggcccg gataactcac gtcgacacag agtcctactt cattgtactc 6300
agtatagcct atccgacgct gtccgagatt aagggggtga ttgtccaccg gctagagggg 6360
gtctcgtaca atataggctc tcaagagtgg tataccactg tgcccaagta tgttgcaacc 6420
caagggtacc ttatctcgaa ttttgatgag tcatcgtgta ctttcatgcc agaggggact 6480
gtgtgcagcc aaaatgcctt gtacccgatg agtcctctgc tccaagaatg cctccggggg 6540
tccaccaagt cctgtgctcg tacactcgta tccgggtctt ttgggaaccg gttcatttta 6600
tcacaaggga acctaatagc caattgtgca tcaatcctct gcaagtgtta cacaacagga 6660
acgatcatta atcaagaccc tgacaagatc ctaacataca ttgctgccga tcactgcccg 6720
gtggtcgagg tgaacggtgt gaccatccaa gtcgggagca ggaggtatcc ggacgcggtg 6780
tacctgcaca gaattgacct cggtcctccc atatcattgg agaggttgga cgtagggaca 6840
aatctgggga atgcaattgc taagttggag gatgccaagg aattgttgga gtcatcggac 6900
cagatattga ggagtatgaa aggtttatcg agcactagca tagtttacat cctgattgca 6960
gtgtgtcttg gagggttgat agggatcccc gctttaatat gttgctgcag ggggcgctgt 7020
aacaaaaagg gagaacaagt tggtatgtca agaccaggcc taaagcctga tcttacaggg 7080
acatcaaaat cctatgtaag gtcgctctga tcctctacaa ctcttgaaac acagatttcc 7140
cacaagtctc ctcttcgtca tcaagcaacc accgcatcca gcatcaagcc cacctgaaat 7200
tgtctccggc ttccctctgg ccgaacgata tcggtagtta attaaaactt agggtgcaag 7260
atcatccaca atgtcaccac aacgagaccg aataaatgcc ttctacaaag acaacccaca 7320
tcctaaggga agtaggatag ttattaacag agaacatctt atgattgata gaccttatgt 7380
tttgctggct gttctattcg tcatgtttct gagcttgatc gggttgctag ccattgcagg 7440
cattagactc catcgtgcag ccatctacac cgcagagatc cataagagcc tcagcaccaa 7500
tctagatgta actaactcga tcgagcatca ggtcaaggac gtgctgacac cactcttcaa 7560
gatcattggt gatgaagtgg gcctgaggac acctcagaga ttcactgacc tagtgaaatt 7620
catctctgac aaaattaaat tccttaatcc ggatagggag tacgacttca gagatctcac 7680
ttggtgtatc aacccgccag agagaatcaa attggattat gatcaatact gtgcagatgt 7740
ggctgctgaa gaactcatga atgcattggt gaactcaact ctactggagg ccagggcaac 7800
caatcagttc ctagctgtct caaagggaaa ctgctcaggg cccactacaa tcagaggtca 7860
attctcaaac atgtcgctgt ccctgttgga cttgtattta agtcgaggtt acaatgtgtc 7920
atctatagtc actatgacat cccagggaat gtacggggga acttacctag tgggaaagcc 7980
taatctgagc agtaaagggt cagagttgtc acaactgagc atgcaccgag tgtttgaagt 8040
aggggttatc agaaatccgg gtttgggggc tccggtgttc catatgacaa actattttga 8100
gcaaccagtc agtaatgatt tcagcaactg catggtggct ttgggggagc ttaaattcgc 8160
agccctctgt cacagggaag attctatcac aattccctat caggggtcag ggaaaggtgt 8220
cagcttccag ctcgtcaagc taggtgtctg gaaatcccca accgacatgc gatcctgggt 8280
ccccctatca acggatgatc cagtgataga taggctttat ctctcatctc acagaggtgt 8340
tatcgctgac aatcaagcaa aatgggctgt cccgacaaca cggacagatg acaagttgcg 8400
aatggagaca tgcttccagc aggcgtgtaa gggtaaaaac caagcactct gcgagaatcc 8460
cgagtgggca ccattgaagg ataacaggat tccttcatac ggggtcttgt ctgttaatct 8520
gagtctgaca gttgagctta aaatcaaaat tgcttcagga ttcgggccat tgatcacaca 8580
cggttcaggg atggacctat acaaaaccaa ccacaacaat gtgtattggc tgactatccc 8640
gccaatgaag aacctagcct taggtgtaat caacacattg gagtggatac cgagattcaa 8700
ggttagtccc aacctcttca ctgttccaat caaggaagca ggcgaggact gccatgcccc 8760
aacataccta cctgcggagg tggatggtga tgtcaaactc agttccaatc tggtaattct 8820
acctggtcag gatctccaat atgttttggc aacctacgat acttccaggg ttgaacatgc 8880
tgtggtttat tatgtttaca gcccaagccg ctcattttct tacttttatc cttttaggtt 8940
gcctataaag ggggtcccaa tcgaattaca agtggaatgc ttcacatggg acaaaaaact 9000
ctggtgccgt cacttctgtg tgcttgcgga ctcagaatct ggtggacata tcactcactc 9060
tgggatggtg ggcatgggag tcagctgcac agtcactcgg gaagatggaa ccaatcgcag 9120
atagggctgc cagtgaaccg atcacatgat gtcacccaga catcaggcat acccactagt 9180
gtgaaataga catcagaatt aagaaaaacg tagggtccaa gtggtttccc gttatggact 9240
cgctatctgt caaccagatc ttataccctg aagttcacct agatagcccg atagttacca 9300
ataagatagt agctatcctg gagtatgctc gagtccctca cgcttacagc ctggaggacc 9360
ctacactgtg tcagaacatc aagcaccgcc taaaaaacgg attctccaac caaatgatta 9420
taaacaatgt ggaagttggg aatgtcatca agtccaagct taggagttat ccggcccact 9480
ctcatattcc atatccaaat tgtaatcagg atttatttaa catagaagac aaagagtcaa 9540
caaggaagat ccgtgagctc ctaaaaaagg gaaattcgct gtactccaaa gtcagtgata 9600
aggttttcca atgcctgagg gacactaact cacggcttgg cctaggctcc gaattgaggg 9660
aggacatcaa ggagaaaatt attaacttgg gagtttacat gcacagctcc caatggtttg 9720
agccctttct gttttggttt acagtcaaga ctgagatgag gtcagtgatt aaatcacaaa 9780
cccatacttg ccataggagg agacacacac ctgtattctt cactggtagt tcagttgagc 9840
tgttaatctc tcgtgacctt gttgctataa tcagtaagga atctcaacat gtatattacc 9900
tgacgtttga actggttttg atgtattgtg atgtcataga ggggaggtta atgacagaga 9960
ccgctatgac cattgatgct aggtatgcag aacttctagg aagagtcaga tacatgtgga 10020
aactgataga tggtttcttc cctgcactcg ggaatccaac ttatcaaatt gtagccatgc 10080
tggagccact ttcacttgct tacctgcaac tgagggatat aacagtagaa ctcagaggtg 10140
ctttccttaa ccactgcttt actgaaatac atgatgttct tgaccaaaac gggttttctg 10200
atgaaggtac ttatcatgag ttaattgaag ccctagatta cattttcata actgatgaca 10260
tacatctgac aggggagatt ttctcatttt tcagaagttt cggccacccc agacttgaag 10320
cagtaacggc tgctgaaaat gtcaggaaat acatgaatca gcctaaagtc attgtgtatg 10380
agactctgat gaaaggtcat gccatatttt gtggaatcat aatcaacggc tatcgtgaca 10440
ggcacggagg cagttggcca cccctgaccc tccccctgca tgctgcagac acaatccgga 10500
atgctcaagc ttcaggtgaa gggttaacac atgagcagtg cgttgataac tggaaatcat 10560
ttgctggagt gagatttggc tgttttatgc ctcttagcct ggacagtgat ctgacaatgt 10620
acctaaagga caaggcactt gctgctctcc aaagggaatg ggattcagtt tacccgaaag 10680
agttcctgcg ttacgatcct cccaagggaa ccgggtcacg gaggcttgta gatgttttcc 10740
ttaatgattc gagctttgac ccatatgata tgataatgta tgtcgtaagt ggagcctacc 10800
tccatgaccc tgagttcaac ctgtcttaca gcctgaaaga aaaggagatc aaggaaacag 10860
gtagactttt cgctaaaatg acttacaaaa tgagggcatg ccaagtgatc gctgaaaatc 10920
taatctcaaa cgggattggc aagtatttta aggacaatgg gatggccaag gatgagcacg 10980
atttgactaa ggcactccac actctggctg tctcaggagt ccccaaagat ctcaaagaaa 11040
gtcacagggg ggggccagtc ttaaaaacct actcccgaag cccagtccac acaagtacca 11100
ggaacgttaa agcagaaaaa gggtttgtag gattccctca tgtaattcgg cagaatcaag 11160
acactgatca tccggagaat atagaaacct acgagacagt cagcgcattt atcacgactg 11220
atctcaagaa gtactgcctt aattggagat atgagaccat cagcttattt gcacagaggc 11280
taaatgagat ttacggatta ccctcatttt ttcagtggct gcataagagg cttgaaacct 11340
ctgtcctcta tgtaagtgac cctcattgcc cccccgacct tgacgcccat gtcccgttat 11400
gcaaagtccc caatgaccaa atcttcatca agtaccctat gggaggtata gaagggtatt 11460
gtcagaagct gtggaccatc agcaccattc cctacttata cctggctgct tatgagagcg 11520
gggtaaggat tgcttcgtta gtgcaagggg acaatcagac catagccgta acaaaaaggg 11580
tacccagcac atggccttac aaccttaaga aacgggaagc tgctagagta actagagatt 11640
actttgtaat tcttaggcaa aggctacatg acattggcca tcacctcaag gcaaatgaga 11700
caattgtttc atcacatttt tttgtctatt caaaaggaat atattatgat gggctacttg 11760
tgtcccaatc actcaagagc atcgcaagat gtgtattctg gtcagagact atagttgatg 11820
aaacaagggc ggcatgcagt aatattgcta caacaatggc caaaagcatt gagagaggtt 11880
atgaccgtta tcttgcatat tccctgaacg tcctaaaagt gatacagcaa attttgatct 11940
cccttggctt cacaatcaat tcaaccatga cccgggatgt agtcataccc ctcctcacaa 12000
acaacgatct cttaataagg atggcactgt tgcccgctcc tattgggggt atgaattatc 12060
tgaatatgag caggctgttt gtcagaaaca tcggtgatcc agtaacatca tcaattgctg 12120
atctcaagag aatgattctc gcatcactaa tgcctgaaga gaccctccat caagtaatga 12180
cacaacaacc gggggactct tcattcctag actgggctag cgacccttac tcagcaaatc 12240
ttgtatgcgt ccagagcatc actagactcc tcaagaacat aactgcaagg tttgtcctga 12300
tccacagtcc aaacccaatg ttaaaagggt tattccatga tgacagcaaa gaagaggacg 12360
agagactggc ggcattcctc atggacaggc atattatagt acctagggca gctcatgaaa 12420
tcctggatca tagtgtcaca ggggcaagag agtctattgc aggcatgcta gataccacaa 12480
aaggcctgat tcgagccagc atgaggaagg gggggttaac ctctcgagtg ataaccagat 12540
tgtccaatta tgactatgaa caatttagag cagggatggt gctattgaca ggaagaaaga 12600
gaaatgtcct cattgacaaa gagtcatgtt cagtgcagct ggctagagcc ctaagaagcc 12660
atatgtgggc aagactagct cgaggacggc ctatttacgg ccttgaggtc cctgatgtac 12720
tagaatctat gcgaggccac cttattcggc gtcatgagac atgtgtcatc tgcgagtgtg 12780
gatcagtcaa ctacggatgg ttttttgtcc cctcgggttg ccaactggat gatattgaca 12840
aggaaacatc atccttgaga gtcccatata ttggttctac cactgatgag agaacagaca 12900
tgaagctcgc cttcgtaaga gccccaagta gatccttgcg atctgccgtt agaatagcaa 12960
cagtgtactc atgggcttac ggtgatgatg atagctcttg gaacgaagcc tggttgttgg 13020
caaggcaaag ggccaatgtg agcctggagg agctaagggt gatcactccc atctcgactt 13080
cgactaattt agcgcatagg ttgagggatc gtagcactca agtgaaatac tcaggtacat 13140
cccttgtccg agtggcaagg tataccacaa tctccaacga caatctctca tttgtcatat 13200
cagataagaa ggttgatact aactttatat accaacaagg aatgcttcta gggttgggtg 13260
ttttagaaac attgtttcga ctcgagaaag atactggatc atctaacacg gtattacatc 13320
ttcacgtcga aacagattgt tgcgtgatcc cgatgataga tcatcccagg atacccagct 13380
cccgcaagct agagctgagg gcagagctat gtaccaaccc attgatatat gataatgcac 13440
ctttaattga cagagatgca acaaggctat acacccagag ccataggagg caccttgtgg 13500
aatttgttac atggtccaca ccccaactat atcacattct agctaagtcc acagcactat 13560
ctatgattga cctggtaaca aaatttgaga aggaccatat gaatgaaatt tcagctctca 13620
taggggatga cgatatcaat agtttcataa ctgagtttct gcttatagag ccaagattat 13680
tcaccatcta cttgggccag tgtgcagcca tcaattgggc atttgatgta cattatcata 13740
gaccatcagg gaaatatcag atgggtgagc tgttgtcttc gttcctttct agaatgagca 13800
aaggagtgtt taaggtgctt gtcaatgctc taagccaccc aaagatctac aagaaattct 13860
ggcattgtgg tattatagag cctatccatg gtccttcact tgatgctcaa aacttgcaca 13920
caactgtgtg caacatggtt tacacatgct atatgaccta cctcgacctg ttgttgaatg 13980
aagagttaga agagttcaca tttcttttgt gtgaaagcga tgaggatgta gtaccggaca 14040
gattcgacaa catccaggca aaacacttgt gtgttctggc agatttgtac tgtcaaccag 14100
ggacctgccc accgattcga ggtctaaggc cggtagagaa atgtgcagtt ctaaccgatc 14160
atatcaaggc agaggctagg ttatctccag caggatcttc gtggaacata aatccaatta 14220
ttgtagacca ttactcatgc tctctgactt atctccgtcg aggatctatc aaacagataa 14280
gattgagagt tgatccagga ttcatttttg acgccctcgc tgaggtaaat gtcagtcagc 14340
caaaggtcgg cagcgacgtc agccatactc tttatgcccc cggtggatat gacattatgg 14400
gatatctgag gcagatcagg aaccggccaa acccgcaggt ggaactgggc cccgtggata 14460
catcctgcgc cttgattctt tgtgacctga aacagaaaga caccccgata gtttacgcga 14520
gtgaagcctt cctctacatg acaggttaca gcaacgcaga ggtgctgggc cggaattgcc 14580
ggtttctgca aagccctgac ggcatggtga agcccaagag cacccggaag tacgtggata 14640
gtaacacaat caatactatg cgcaaggcaa tcgacaggaa tgccgaggtg caggttgaag 14700
tagtcaattt taaaaagaat ggacagcgat ttgttaattt cctgactatg atacctgtta 14760
gggacgaaac aggcgagtat cgatactcta tgggattcca gtgcgaaaca gaataagacg 14820
tcatctcaaa tatgagcatc aaggatttca gacctccaca cgatgatgtt gcaaaattgc 14880
tcaaagatat caacacaagc aagcacaatc ttcccatttc agggggtagt ctcgccaatt 14940
atgaaatcca tgctttccgc agaatcgggt taaactcatc tgcttgctac aaagctgttg 15000
agatatcaac attaattagg agatgccttg agccagggga agacggcttg ttcttgggtg 15060
aggggtcggg ttctatgttg atcacttata aggagatact aaaactaaac aagtgcttct 15120
ataatagtgg ggtttccgcc aattctagat ctggtcaaag ggaattagca ccctatccct 15180
ccgaagttgg ccttgtcgaa cacagaatgg gagtaggtaa tattgtcaag gtgctcttta 15240
acgggaggcc cgaagtcacg tgggtaggca gtatagattg cttcaatttc atagtcagta 15300
atatccctac ctctagtgtg gggtttatcc attcagatat agagacctta cctaacaaag 15360
atactataga gaagctagag gaattggcag ccatcttatc gatggctcta ctccttggca 15420
aaataggatc aatactggtg attaagctta tgcctttcag cggggatttt gttcagggat 15480
ttataagcta tgtagggtct cattatagag aagtgaacct tgtctaccct aggtacagca 15540
acttcatatc tactgaatct tatttagtta tgacagatct caaagctaac cggctaatga 15600
atcctgaaaa gatcaagcag cagataattg aatcatctgt acggacttca cctggactta 15660
taggtcacat cctatccatt aagcaactaa gctgcataca agcaattgtg ggaggcgcgg 15720
ttagtagagg tgatatcaac cctattctga aaaaacttac acctatagag caggtgctga 15780
tcagttgcgg gttggcaatt aacggaccta aactgtgcaa agaattaatc caccatgatg 15840
ttgcctcagg gcaagatgga ttgcttaact ctatactcat cctctacagg gagttggcaa 15900
gattcaaaga caaccaaaga agtcaacaag ggatgttcca cgcttacccc gtattggtaa 15960
gtagtaggca acgagaactt gtatctagga tcactcgcaa attttggggg catattcttc 16020
tttactccgg gaacagaaag ttgataaatc ggtttatcca gaatctcaag tccggttatc 16080
tagtactaga cttacaccag aatatcttcg ttaagaatct atccaagtca gagaaacaga 16140
ttattatgac ggggggttta aaacgtgagt gggtttttaa ggtaacagtc aaggagacca 16200
aagaatggta caagttagtc ggatacagcg ctctgattaa ggattaattg gttgaactcc 16260
ggaaccctaa tcctgcccta ggtagttagg cattatttgc aatatattaa agaaaacttt 16320
gaaaatacga agtttctatt cccagctttg tctggt 16356
<210> 75
<211> 12114
<212> DNA
<213> Artificial Sequence
<220>
<223> nmaghigh Lc 质粒
<400> 75
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
acgagatggc cacacttttg aggagcttag cattgttcaa aagaaacaag gacaaaccac 1020
ccattacatc aggatccggt ggagccatca gaggaatcaa acacattatt atagtaccaa 1080
ttcctggaga ttcctcaatt accactcgat ccagactact ggaccggttg gtcaggttaa 1140
ttggaaaccc ggatgtgagc gggcccaaac taacaggggc actaataggt atattatcct 1200
tatttgtgga gtctccaggt caattgattc agaggatcac cgatgaccct gacgttagca 1260
tcaggctgtt agaggttgtt cagagtgacc agtcacaatc tggccttacc ttcgcatcaa 1320
gaggtaccaa catggaggat gaggcggacc aatacttttc acatgatgat ccaagcagta 1380
gtgatcaatc caggtccgga tggttcgaga acaaggaaat ctcagatatt gaagtgcaag 1440
accctgaggg attcaacatg attctgggta ccattctagc ccagatctgg gtcttgctcg 1500
caaaggcggt tacggcccca gacacggcag ctgattcgga gctaagaagg tggataaagt 1560
acacccaaca aagaagggta gttggtgaat ttagattgga gagaaaatgg ttggatgtgg 1620
tgaggaacag gattgccgag gacctctctt tacgccgatt catggtggct ctaatcctgg 1680
atatcaagag gacacccggg aacaaaccta ggattgctga aatgatatgt gacattgata 1740
catatatcgt agaggcagga ttagccagtt ttatcctgac tattaagttt gggatagaaa 1800
ctatgtatcc tgctcttgga ctgcatgaat ttgctggtga gttatccaca cttgagtcct 1860
tgatgaatct ttaccagcaa atgggagaaa ctgcacccta catggtaatc ctagagaact 1920
caattcagaa caagttcagt gcaggatcat accctctgct ctggagctat gccatgggag 1980
taggagtgga acttgaaaac tccatgggag gtttgaactt tggtcgatct tactttgatc 2040
cagcatattt tagattaggg caagagatgg tgaggaggtc agctggaaag gtcagttcca 2100
cattggcatc cgaactcggt atcactgccg aggatgcaag gcttgtttca gagattgcaa 2160
tgcatactac tgaggacagg atcagtagag cggtcggacc cagacaagcc caagtgtcat 2220
ttctacacgg tgatcaaagt gagaatgagc taccaggatt ggggggcaag gaagatagga 2280
gggtcaaaca gggtcgggga gaagccaggg agagctacag agaaaccggg tccagcagag 2340
caagtgatgc gagagctgcc catcctccaa ccagcatgcc cctagacatt gacactgcat 2400
cggagtcagg ccaagatccg caggacagtc gaaggtcagc tgacgccctg ctcaggctgc 2460
aagccatggc aggaatcttg gaagaacaag gctcagacac ggacacccct agggtataca 2520
atgacagaga tcttctagac taggtgcgag aggccgagga ccagaacaac atccgcctac 2580
cctccatcat tgttataaaa aacttaggaa ccaggtccac acagccgcca gccaaccaac 2640
catccactcc cacgactgga gccgatggca gaagagcagg cacgccatgt caaaaacgga 2700
ctggaatgca tccgggctct caaggccgag cccatcggct cactggccgt cgaggaagcc 2760
atggcagcat ggtcagaaat atcagacaac ccaggacagg accgagccac ctgcaaggaa 2820
gaggaggcag gcagttcggg tctcagcaaa ccatgcctct cagcaattgg atcaactgaa 2880
ggcggtgcac ctcgcatccg cggtcaggga tctggagaaa gcgatgacga cgctgaaact 2940
ttgggaatcc cctcaagaaa tctccaggca tcaagcactg ggttacagtg ttatcatgtt 3000
tatgatcaca gcggtgaagc ggttaaggga atccaagatg ctgactctat catggttcaa 3060
tcaggccttg atggtgatag caccctctca ggaggagacg atgaatctga aaacagcgat 3120
gtggatattg gcgaacctga taccgaggga tatgctatca ctgaccgggg atctgctccc 3180
atctctatgg ggttcagggc ttctgatgtt gaaactgcag aaggagggga gatccacgag 3240
ctcctgaaac tccaatccag aggcaacaac tttccgaagc ttgggaaaac tctcaatgtt 3300
cctccgcccc cgaaccccag tagggccagc acttccgaga cacccattaa aaagggcaca 3360
gacgcgagat tggcctcatt tggaacggag atcgcgtctt tattgacagg tggtgcaacc 3420
caatgtgctc gaaagtcacc ctcggaacca tcagggccag gtgcacctgc ggggaatgtc 3480
cccgagtgtg tgagcaatgc cgcactgata caggagtgga cacccgaatc tggtaccaca 3540
atctccccga gatcccagaa taatgaagaa gggggagact attatgatga tgagctgttc 3600
tccgatgtcc aagacatcaa aacagccttg gccaaaatac acgaggataa tcagaagata 3660
atctccaagc tagaatcatt gctgttattg aagggagaag ttgagtcaat taagaagcag 3720
atcaacaggc aaaatatcag catatccacc ctggaaggac acctctcaag catcatgatt 3780
gccattcctg gacttgggaa ggatcccaac gaccccactg cagatgtcga actcaatccc 3840
gacctgaaac ccatcatagg cagagattca ggccgagcac tggccgaagt tctcaagaag 3900
cccgttgcca gccgacaact ccagggaatg actaatggac ggaccagttc cagaggacag 3960
ctgctgaagg aatttcaact aaagccgatc gggaaaaagg tgagctcagc cgtcgggttt 4020
gttcctgaca ccggccctgc atcacgcagt gtaatccgct ccattataaa atccagccgg 4080
ctagaggagg atcggaagcg ttacctgatg actctccttg atgatatcaa aggagccaac 4140
gatcttgcca agttccacca gatgctgatg aagataataa tgaagtagct acagctcaac 4200
ttacctgcca accccatgcc agtcgaccta attagtacaa cctaaatcca ttataaaaaa 4260
cttaggagca aagtgattgc ctcctaagtt ccacaatgac agagatctac gatttcgaca 4320
agtcggcatg ggacatcaaa gggtcgatcg ctccgataca acctaccacc tacagtgatg 4380
gcaggctggt gccccaggtc agagtcatag atcctggtct aggtgatagg aaggatgaat 4440
gctttatgta catgtttctg ctgggggttg ttgaggacag cgatccccta gggcctccaa 4500
tcgggcgagc attcgggtcc ctgcccttag gtgttggtag atccacagca aaacccgagg 4560
aactcctcaa agaggccact gagcttgaca tagttgttag acgtacagca gggctcaatg 4620
aaaaactggt gttctacaac aacaccccac taaccctcct cacaccttgg agaaaggtcc 4680
taacaacagg gagtgtcttc aatgcaaacc aagtgtgcaa tgcggttaat ctaataccgc 4740
tggacacccc gcagaggttc cgtgttgttt atatgagcat cacccgtctt tcggataacg 4800
ggtattacac cgttcccaga agaatgctgg aattcagatc ggtcaatgca gtggccttca 4860
acctgctagt gacccttagg attgacaagg cgattggccc tgggaagatc atcgacaatg 4920
cagagcaact tcctgaggca acatttatgg tccacatcgg gaacttcagg agaaagaaga 4980
gtgaagtcta ctctgccgat tattgcaaaa tgaaaatcga aaagatgggc ctggtttttg 5040
cacttggtgg gatagggggc accagtcttc acattagaag cacaggcaaa atgagcaaga 5100
ctctccatgc acaactcggg ttcaagaaga ccttatgtta cccactgatg gatatcaatg 5160
aagaccttaa tcggttactc tggaggagca gatgcaagat agtaagaatc caggcagttt 5220
tgcagccatc agttcctcaa gaattccgca tttacgacga cgtgatcata aatgatgacc 5280
aaggactatt caaagttctg tagaccgcag tgcccagcaa tacccgaaaa cgacccccct 5340
cataatgaca gccagaaggc ccggacaaaa aagccccctc cagaagactc cacggaccaa 5400
gcgagaggcc agccagcagc cgacagcaag tgtggacacc aggcggccca agcacagaac 5460
agccccgaca caaggccacc accagccatc ccaatctgcg tcctcctcgt gggacccccg 5520
aggaccaacc ccgaaggtcg ctccgaacac agaccaccaa ccgcatcccc acagctcccg 5580
ggaaaggaac ccccagcaac tggaaggccc ctccccccct cccccaacgc aagaacccca 5640
caaccgaacc gcacaagcga ccgaggtgac ccaaccgcag gcatccgact ccttagacag 5700
atcctctccc cccggcatac taaacaaaac ttagggccaa ggaacacaca cactcgacag 5760
aacccagacc ccggcccgcg gcaccgcgcc cccacccccc gaaaaccaga gggagccccc 5820
aaccaaaccc gccggccccc ccggtgccca caggtaggca caccaacccc cgaccagacc 5880
cagcacccag ccaccgacaa tccaagacgg ggggcccccc ccaaaaaaag gcccccaggg 5940
gccgacagcc agcatcgcga ggaagcacac ccaccccaca cacgaccacg gcaaccgaac 6000
cagagtccag accaccctgg gccaccagct cccagactcg gccatcaccc cgcaaaaagg 6060
aaaggccaca acccgcgcac cccagccccg atccggcggg cagccactca acccgaacca 6120
gcacccaaga gcgatccctg ggggaccccc aaaccgcaaa agacatcagt atcccacagc 6180
ctctccaagt cccccggtct cctcctcttc tcgaagggac caaaagatca atccaccaca 6240
tccgacgaca ctcaattccc cacccccaaa ggagacaccg ggaatcccag aatcaagact 6300
catccagtgt ccatcatggg tctcaaggtg aacgtctctg ccatattcat ggcagtactg 6360
ttaactctcc aaacacccac cggtcaaatc cattggggca atctctctaa gataggggtg 6420
gtagggatag gaagtgcaag ctacaaagtt atgactcgtt ccagccatca atcattggtc 6480
ataaaattaa tgcccaatat aactctcctc aataactgca cgagggtaga gatcgcagaa 6540
tacaggagac tactgagaac agttttggaa ccaattagag atgcacttaa tgcaatgacc 6600
cagaatataa gaccggttca gagtgtagct tcaagtagga gacacaagag atttgcggga 6660
gttgtcctgg caggtgcggc cctaggcgtt gccacagctg ctcagataac agccggcatt 6720
gcacttcacc agtccatgct gaactctcaa gccatcgaca atctgagagc aagcctggaa 6780
actactaatc aggcaattga ggcaatcaga caagcagggc aggagatgat attggctgtt 6840
cagggtgtcc aagactacat caataatgag ctgataccgt ctatgaacca actatcttgt 6900
gatttaatcg gccagaagct agggctcaaa ttgctcagat actatacaga aatcctgtca 6960
ttatttggcc ccagcttacg ggaccccata tctgcggaga tatccatcca ggctttgagc 7020
tatgcgcttg gaggagatat caataaggta ttagaaaagc tcggatacag tggaggtgat 7080
ttactgggca tcttagagag cagaggaata aaggcccgga taactcacgt cgacacagag 7140
tcctacttca ttgtactcag tatagcctat ccgacgctgt ccgagattaa gggggtgatt 7200
gtccaccggc tagagggggt ctcgtacaat ataggctctc aagagtggta taccactgtg 7260
cccaagtatg ttgcaaccca agggtacctt atctcgaatt ttgatgagtc atcgtgtact 7320
ttcatgccag aggggactgt gtgcagccaa aatgccttgt acccgatgag tcctctgctc 7380
caagaatgcc tccgggggtc caccaagtcc tgtgctcgta cactcgtatc cgggtctttt 7440
gggaaccggt tcattttatc acaagggaac ctaatagcca attgtgcatc aatcctctgc 7500
aagtgttaca caacaggaac gatcattaat caagaccctg acaagatcct aacatacatt 7560
gctgccgatc actgcccggt ggtcgaggtg aacggtgtga ccatccaagt cgggagcagg 7620
aggtatccgg acgcggtgta cctgcacaga attgacctcg gtcctcccat atcattggag 7680
aggttggacg tagggacaaa tctggggaat gcaattgcta agttggagga tgccaaggaa 7740
ttgttggagt catcggacca gatattgagg agtatgaaag gtttatcgag cactagcata 7800
gtttacatcc tgattgcagt gtgtcttgga gggttgatag ggatccccgc tttaatatgt 7860
tgctgcaggg ggcgctgtaa caaaaaggga gaacaagttg gtatgtcaag accaggccta 7920
aagcctgatc ttacagggac atcaaaatcc tatgtaaggt cgctctgatc ctctacaact 7980
cttgaaacac agatttccca caagtctcct cttcgtcatc aagcaaccac cgcatccagc 8040
atcaagccca cctgaaattg tctccggctt ccctctggcc gaacgatatc ggtagttaat 8100
taaaacttag ggtgcaagat catccacaat gtcaccacaa cgagaccgaa taaatgcctt 8160
ctacaaagac aacccacatc ctaagggaag taggatagtt attaacagag aacatcttat 8220
gattgataga ccttatgttt tgctggctgt tctattcgtc atgtttctga gcttgatcgg 8280
gttgctagcc attgcaggca ttagactcca tcgtgcagcc atctacaccg cagagatcca 8340
taagagcctc agcaccaatc tagatgtaac taactcgatc gagcatcagg tcaaggacgt 8400
gctgacacca ctcttcaaga tcattggtga tgaagtgggc ctgaggacac ctcagagatt 8460
cactgaccta gtgaaattca tctctgacaa aattaaattc cttaatccgg atagggagta 8520
cgacttcaga gatctcactt ggtgtatcaa cccgccagag agaatcaaat tggattatga 8580
tcaatactgt gcagatgtgg ctgctgaaga actcatgaat gcattggtga actcaactct 8640
actggaggcc agggcaacca atcagttcct agctgtctca aagggaaact gctcagggcc 8700
cactacaatc agaggtcaat tctcaaacat gtcgctgtcc ctgttggact tgtatttaag 8760
tcgaggttac aatgtgtcat ctatagtcac tatgacatcc cagggaatgt acgggggaac 8820
ttacctagtg ggaaagccta atctgagcag taaagggtca gagttgtcac aactgagcat 8880
gcaccgagtg tttgaagtag gggttatcag aaatccgggt ttgggggctc cggtgttcca 8940
tatgacaaac tattttgagc aaccagtcag taatgatttc agcaactgca tggtggcttt 9000
gggggagctt aaattcgcag ccctctgtca cagggaagat tctatcacaa ttccctatca 9060
ggggtcaggg aaaggtgtca gcttccagct cgtcaagcta ggtgtctgga aatccccaac 9120
cgacatgcga tcctgggtcc ccctatcaac ggatgatcca gtgatagata ggctttatct 9180
ctcatctcac agaggtgtta tcgctgacaa tcaagcaaaa tgggctgtcc cgacaacacg 9240
gacagatgac aagttgcgaa tggagacatg cttccagcag gcgtgtaagg gtaaaaacca 9300
agcactctgc gagaatcccg agtgggcacc attgaaggat aacaggattc cttcatacgg 9360
ggtcttgtct gttaatctga gtctgacagt tgagcttaaa atcaaaattg cttcaggatt 9420
cgggccattg atcacacacg gttcagggat ggacctatac aaaaccaacc acaacaatgt 9480
gtattggctg actatcccgc caatgaagaa cctagcctta ggtgtaatca acacattgga 9540
gtggataccg agattcaagg ttagtcccaa cctcttcact gttccaatca aggaagcagg 9600
cgaggactgc catgccccaa catacctacc tgcggaggtg gatggtgatg tcaaactcag 9660
ttccaatctg gtaattctac ctggtcagga tctccaatat gttttggcaa cctacgatac 9720
ttccagggtt gaacatgctg tggtttatta tgtttacagc ccaagccgct cattttctta 9780
cttttatcct tttaggttgc ctataaaggg ggtcccaatc gaattacaag tggaatgctt 9840
cacatgggac aaaaaactct ggtgccgtca cttctgtgtg cttgcggact cagaatctgg 9900
tggacatatc actcactctg ggatggtggg catgggagtc agctgcacag tcactcggga 9960
agatggaacc aatcgcagat agggctgcca gtgaaccgat cacatgatgt cacccagaca 10020
tcaggcatac ccactagtgt gaaatagaca tcagaattaa gaaaaacgta gggtccaagt 10080
ggtttcccgt tgacgtcatg catactcttt atgcccccgg tggatatgac attatgggat 10140
atctggacca gatcggcaac cggccaaacc cgcaggtgga actgggcccc gtggatacat 10200
cctgcgcctt gattctttgt gacctgaaac agaaagacac cccgatagtt tacgcgagtg 10260
aagccttcct ctacatgaca ggttacagca acgcagaggt gctgggccgg aattgccggt 10320
ttctgcaaag ccctgacggc atggtgaagc ccaagagcac ccggaagtac gtggatagta 10380
acacaatcaa tactatccgc aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag 10440
tcaattttaa aaagaatgga cagcgatttg ttaatttcct gactatcata cctgttaggg 10500
acgaaacagg cgagtatcga tactctatgg gattccagtg cgaaacagaa tatccgtacg 10560
acgtcccaga ttacgcgggc atctcaaata tgagcatcaa ggatttcaga cctccacacg 10620
atgatgttgc aaaattgctc aaagatatca acacaagcaa gcacaatctt cccatttcag 10680
ggggtagtct cgccaattat gaaatccatg ctttccgcag aatcgggtta aactcatctg 10740
cttgctacaa agctgttgag atatcaacat taattaggag atgccttgag ccaggggaag 10800
acggcttgtt cttgggtgag gggtcgggtt ctatgttgat cacttataag gagatactaa 10860
aactaaacaa gtgcttctat aatagtgggg tttccgccaa ttctagatct ggtcaaaggg 10920
aattagcacc ctatccctcc gaagttggcc ttgtcgaaca cagaatggga gtaggtaata 10980
ttgtcaaggt gctctttaac gggaggcccg aagtcacgtg ggtaggcagt atagattgct 11040
tcaatttcat agtcagtaat atccctacct ctagtgtggg gtttatccat tcagatatag 11100
agaccttacc taacaaagat actatagaga agctagagga attggcagcc atcttatcga 11160
tggctctact ccttggcaaa ataggatcaa tactggtgat taagcttatg cctttcagcg 11220
gggattttgt tcagggattt ataagctatg tagggtctca ttatagagaa gtgaaccttg 11280
tctaccctag gtacagcaac ttcatatcta ctgaatctta tttagttatg acagatctca 11340
aagctaaccg gctaatgaat cctgaaaaga tcaagcagca gataattgaa tcatctgtac 11400
ggacttcacc tggacttata ggtcacatcc tatccattaa gcaactaagc tgcatacaag 11460
caattgtggg aggcgcggtt agtagaggtg atatcaaccc tattctgaaa aaacttacac 11520
ctatagagca ggtgctgatc agttgcgggt tggcaattaa cggacctaaa ctgtgcaaag 11580
aattaatcca ccatgatgtt gcctcagggc aagatggatt gcttaactct atactcatcc 11640
tctacaggga gttggcaaga ttcaaagaca accaaagaag tcaacaaggg atgttccacg 11700
cttaccccgt attggtaagt agtaggcaac gagaacttgt atctaggatc actcgcaaat 11760
tttgggggca tattcttctt tactccggga acagaaagtt gataaatcgg tttatccaga 11820
atctcaagtc cggttatcta gtactagact tacaccagaa tatcttcgtt aagaatctat 11880
ccaagtcaga gaaacagatt attatgacgg ggggtttaaa acgtgagtgg gtttttaagg 11940
taacagtcaa ggagaccaaa gaatggtaca agttagtcgg atacagcgct ctgattaagg 12000
attaattggt tgaactccgg aaccctaatc ctgccctagg tagttaggca ttatttgcaa 12060
tatattaaag aaaactttga aaatacgaag tttctattcc cagctttgtc tggt 12114
<210> 76
<211> 450
<212> DNA
<213> Artificial Sequence
<220>
<223> pMagFast1
<400> 76
catactcttt atgcccccgg tggatatgac attatgggat atctgaggca gatcaggaac 60
cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt 120
gacctgaaac agaaagacac cccggtggtt tacgcgagtg aagccttcct ctacatgaca 180
ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc 240
atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc 300
aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga 360
cagcgatttg ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga 420
tactctatgg gattccagtg cgaaacagaa 450
<210> 77
<211> 450
<212> DNA
<213> Artificial Sequence
<220>
<223> pMagFast2
<400> 77
catactcttt atgcccccgg tggatatgac attatgggat atctgaggca gatcaggaac 60
cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt ggtgctttgt 120
gacctgaaac agaaagacac cccggtggtt tacgcgagtg aagccttcct ctacatgaca 180
ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc 240
atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc 300
aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga 360
cagcgatttg ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga 420
tactctatgg gattccagtg cgaaacagaa 450
<210> 78
<211> 903
<212> DNA
<213> Artificial Sequence
<220>
<223> pMag-direct-nMaghigh
<400> 78
catactcttt atgcccccgg tggatatgac attatgggat atctgaggca gatcaggaac 60
cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt 120
gacctgaaac agaaagacac cccgatagtt tacgcgagtg aagccttcct ctacatgaca 180
ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc 240
atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc 300
aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga 360
cagcgatttg ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga 420
tactctatgg gattccagtg cgaaacagaa atgcacactc tgtatgctcc aggtggctat 480
gacatcatgg gctatctgga tcagataggg aatcgtccta atccccaagt ggaacttgga 540
ccagtagaca ctagctgtgc actgattctc tgtgacctca agcagaagga cacacccatc 600
gtgtacgcct ctgaagcgtt tctgtacatg acaggctaca gcaatgccga agtcttgggg 660
agaaattgcc gatttctgca gagtcctgac ggtatggtca aacccaagtc aacgaggaaa 720
tacgtcgatt ccaacaccat caacaccatt cggaaagcca tcgatcggaa tgctgaggtg 780
caagtggagg ttgtgaactt caagaagaac ggacagaggt tcgtgaactt cctgaccata 840
attccggtta gagatgagac aggagagtac cgctattcca tggggttcca gtgcgaaaca 900
gaa 903
<210> 79
<211> 981
<212> DNA
<213> Artificial Sequence
<220>
<223> pMag-linker-nMag
<400> 79
catactcttt atgcccccgg tggatatgac attatgggat atctgaggca gatcaggaac 60
cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt 120
gacctgaaac agaaagacac cccgatagtt tacgcgagtg aagccttcct ctacatgaca 180
ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc 240
atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc 300
aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga 360
cagcgatttg ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga 420
tactctatgg gattccagtg cgaaacagaa accggtaatt ccgctgacgg cggcggagga 480
tcgggtggta gtggtggttc aggaggagga tcgacccaag gaggatccat gcatactctt 540
tatgcccccg gtggatatga cattatggga tatctggacc agatcggcaa ccggccaaac 600
ccgcaggtgg aactgggccc cgtggataca tcctgcgcct tgattctttg tgacctgaaa 660
cagaaagaca ccccgatagt ttacgcgagt gaagccttcc tctacatgac aggttacagc 720
aacgcagagg tgctgggccg gaattgccgg tttctgcaaa gccctgacgg catggtgaag 780
cccaagagca cccggaagta cgtggatagt aacacaatca atactatgcg caaggcaatc 840
gacaggaatg ccgaggtgca ggttgaagta gtcaatttta aaaagaatgg acagcgattt 900
gttaatttcc tgactatgat acctgttagg gacgaaacag gcgagtatcg atactctatg 960
ggattccagt gcgaaacaga a 981
<210> 80
<211> 1245
<212> DNA
<213> Artificial Sequence
<220>
<223> pMag-4xlinker-nMaghigh
<400> 80
catactcttt atgcccccgg tggatatgac attatgggat atctgaggca gatcaggaac 60
cggccaaacc cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt 120
gacctgaaac agaaagacac cccgatagtt tacgcgagtg aagccttcct ctacatgaca 180
ggttacagca acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc 240
atggtgaagc ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc 300
aaggcaatcg acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga 360
cagcgatttg ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga 420
tactctatgg gattccagtg cgaaacagaa ggatccacgg ggaatagcgc tgatggtgga 480
ggcggtagcg gtggttctgg tgggagtggc ggaggatcaa cacaaggccg gaccggcgga 540
ctgaagggct cttcaggcag cagcgagaac ctctactttc agagcggctc aagcgggagt 600
tctgggagtg gacggaccgg cactggcaat agcgcagatg gcggaggagg atctggcggg 660
agcggaggga gtggcggcgg gtctacacag ggaggccggc caggcctgaa aggcagctcc 720
ggttcctctg agaacctgta cttccagtct ggcagttctg gctcctcagg aagcggacct 780
gcaggcggat ccatgcatac tctttatgcc cccggtggat atgacattat gggatatctg 840
gaccagatcg gcaaccggcc aaacccgcag gtggaactgg gccccgtgga tacatcctgc 900
gccttgattc tttgtgacct gaaacagaaa gacaccccga tagtttacgc gagtgaagcc 960
ttcctctaca tgacaggtta cagcaacgca gaggtgctgg gccggaattg ccggtttctg 1020
caaagccctg acggcatggt gaagcccaag agcacccgga agtacgtgga tagtaacaca 1080
atcaatacta tccgcaaggc aatcgacagg aatgccgagg tgcaggttga agtagtcaat 1140
tttaaaaaga atggacagcg atttgttaat ttcctgacta tcatacctgt tagggacgaa 1200
acaggcgagt atcgatactc tatgggattc cagtgcgaaa cagaa 1245
<210> 81
<211> 20942
<212> DNA
<213> Artificial Sequence
<220>
<223> p(+)MV323-pMag-4xlinker-nMaghigh
<400> 81
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
acgagatggc cacacttttg aggagcttag cattgttcaa aagaaacaag gacaaaccac 1020
ccattacatc aggatccggt ggagccatca gaggaatcaa acacattatt atagtaccaa 1080
ttcctggaga ttcctcaatt accactcgat ccagactact ggaccggttg gtcaggttaa 1140
ttggaaaccc ggatgtgagc gggcccaaac taacaggggc actaataggt atattatcct 1200
tatttgtgga gtctccaggt caattgattc agaggatcac cgatgaccct gacgttagca 1260
tcaggctgtt agaggttgtt cagagtgacc agtcacaatc tggccttacc ttcgcatcaa 1320
gaggtaccaa catggaggat gaggcggacc aatacttttc acatgatgat ccaagcagta 1380
gtgatcaatc caggtccgga tggttcgaga acaaggaaat ctcagatatt gaagtgcaag 1440
accctgaggg attcaacatg attctgggta ccattctagc ccagatctgg gtcttgctcg 1500
caaaggcggt tacggcccca gacacggcag ctgattcgga gctaagaagg tggataaagt 1560
acacccaaca aagaagggta gttggtgaat ttagattgga gagaaaatgg ttggatgtgg 1620
tgaggaacag gattgccgag gacctctctt tacgccgatt catggtggct ctaatcctgg 1680
atatcaagag gacacccggg aacaaaccta ggattgctga aatgatatgt gacattgata 1740
catatatcgt agaggcagga ttagccagtt ttatcctgac tattaagttt gggatagaaa 1800
ctatgtatcc tgctcttgga ctgcatgaat ttgctggtga gttatccaca cttgagtcct 1860
tgatgaatct ttaccagcaa atgggagaaa ctgcacccta catggtaatc ctagagaact 1920
caattcagaa caagttcagt gcaggatcat accctctgct ctggagctat gccatgggag 1980
taggagtgga acttgaaaac tccatgggag gtttgaactt tggtcgatct tactttgatc 2040
cagcatattt tagattaggg caagagatgg tgaggaggtc agctggaaag gtcagttcca 2100
cattggcatc cgaactcggt atcactgccg aggatgcaag gcttgtttca gagattgcaa 2160
tgcatactac tgaggacagg atcagtagag cggtcggacc cagacaagcc caagtgtcat 2220
ttctacacgg tgatcaaagt gagaatgagc taccaggatt ggggggcaag gaagatagga 2280
gggtcaaaca gggtcgggga gaagccaggg agagctacag agaaaccggg tccagcagag 2340
caagtgatgc gagagctgcc catcctccaa ccagcatgcc cctagacatt gacactgcat 2400
cggagtcagg ccaagatccg caggacagtc gaaggtcagc tgacgccctg ctcaggctgc 2460
aagccatggc aggaatcttg gaagaacaag gctcagacac ggacacccct agggtataca 2520
atgacagaga tcttctagac taggtgcgag aggccgagga ccagaacaac atccgcctac 2580
cctccatcat tgttataaaa aacttaggaa ccaggtccac acagccgcca gccaaccaac 2640
catccactcc cacgactgga gccgatggca gaagagcagg cacgccatgt caaaaacgga 2700
ctggaatgca tccgggctct caaggccgag cccatcggct cactggccgt cgaggaagcc 2760
atggcagcat ggtcagaaat atcagacaac ccaggacagg accgagccac ctgcaaggaa 2820
gaggaggcag gcagttcggg tctcagcaaa ccatgcctct cagcaattgg atcaactgaa 2880
ggcggtgcac ctcgcatccg cggtcaggga tctggagaaa gcgatgacga cgctgaaact 2940
ttgggaatcc cctcaagaaa tctccaggca tcaagcactg ggttacagtg ttatcatgtt 3000
tatgatcaca gcggtgaagc ggttaaggga atccaagatg ctgactctat catggttcaa 3060
tcaggccttg atggtgatag caccctctca ggaggagacg atgaatctga aaacagcgat 3120
gtggatattg gcgaacctga taccgaggga tatgctatca ctgaccgggg atctgctccc 3180
atctctatgg ggttcagggc ttctgatgtt gaaactgcag aaggagggga gatccacgag 3240
ctcctgaaac tccaatccag aggcaacaac tttccgaagc ttgggaaaac tctcaatgtt 3300
cctccgcccc cgaaccccag tagggccagc acttccgaga cacccattaa aaagggcaca 3360
gacgcgagat tggcctcatt tggaacggag atcgcgtctt tattgacagg tggtgcaacc 3420
caatgtgctc gaaagtcacc ctcggaacca tcagggccag gtgcacctgc tgggaatgtc 3480
cccgagtgtg tgagcaatgc cgcactgata caggagtgga cacccgaatc tggtaccaca 3540
atctccccga gatcccagaa taatgaagaa gggggagact attatgatga tgagctgttc 3600
tccgatgtcc aagacatcaa aacagccttg gccaaaatac acgaggataa tcagaagata 3660
atctccaagc tagaatcatt gctgttattg aagggagaag ttgagtcaat taagaagcag 3720
atcaacaggc aaaatatcag catatccacc ctggaaggac acctctcaag catcatgatt 3780
gccattcctg gacttgggaa ggatcccaac gaccccactg cagatgtcga actcaatccc 3840
gacctgaaac ccatcatagg cagagattca ggccgagcac tggccgaagt tctcaagaag 3900
cccgttgcca gccgacaact ccagggaatg actaatggac ggaccagttc cagaggacag 3960
ctgctgaagg aatttcaact aaagccgatc gggaaaaagg tgagctcagc cgtcgggttt 4020
gttcctgaca ccggccctgc atcacgcagt gtaatccgct ccattataaa atccagccgg 4080
ctagaggagg atcggaagcg ttacctgatg actctccttg atgatatcaa aggagccaac 4140
gatcttgcca agttccacca gatgctgatg aagataataa tgaagtagct acagctcaac 4200
ttacctgcca accccatgcc agtcgaccta attagtacaa cctaaatcca ttataaaaaa 4260
cttaggagca aagtgattgc ctcctaagtt ccacaatgac agagatctac gatttcgaca 4320
agtcggcatg ggacatcaaa gggtcgatcg ctccgataca acctaccacc tacagtgatg 4380
gcaggctggt gccccaggtc agagtcatag atcctggtct aggtgatagg aaggatgaat 4440
gctttatgta catgtttctg ctgggggttg ttgaggacag cgattcccta gggcctccaa 4500
tcgggcgagc attcgggtcc ctgcccttag gtgttggtag atccacagca aaacccgaga 4560
aactcctcaa agaggccact gagcttgaca tagttgttag acgtacagca gggctcaatg 4620
aaaaactggt gttctacaac aacaccccac taaccctcct cacaccttgg agaaaggtcc 4680
taacaacagg gagtgtcttc aatgcaaacc aagtgtgcaa tgcggttaat ctaataccgc 4740
tggacacccc gcagaggttc cgtgttgttt atatgagcat cacccgtctt tcggataacg 4800
ggtattacac cgttcccaga agaatgctgg aattcagatc ggtcaatgca gtggccttca 4860
acctgctagt gacccttagg attgacaagg cgattggccc tgggaagatc atcgacaatg 4920
cagagcaact tcctgaggca acatttatgg tccacatcgg gaacttcagg agaaagaaga 4980
gtgaagtcta ctctgccgat tattgcaaaa tgaaaatcga aaagatgggc ctggtttttg 5040
cacttggtgg gatagggggc accagtcttc acattagaag cacaggcaaa atgagcaaga 5100
ctctccatgc acaactcggg ttcaagaaga ccttatgtta cccactgatg gatatcaatg 5160
aagaccttaa tcggttactc tggaggagca gatgcaagat agtaagaatc caggcagttt 5220
tgcagccatc agttcctcaa gaattccgca tttacgacga cgtgatcata aatgatgacc 5280
aaggactatt caaagttctg tagaccgcag tgcccagcaa tacccgaaaa cgacccccct 5340
cataatgaca gccagaaggc ccggacaaaa aagccccctc cagaagactc cacggaccaa 5400
gcgagaggcc agccagcagc cgacagcaag tgtggacacc aggcggccca agcacagaac 5460
agccccgaca caaggccacc accagccatc ccaatctgcg tcctcctcgt gggacccccg 5520
aggaccaacc ccgaaggtcg ctccgaacac agaccaccaa ccgcatcccc acagctcccg 5580
ggaaaggaac ccccagcaac tggaaggccc ctccccccct cccccaacgc aagaacccca 5640
caaccgaacc gcacaagcga ccgaggtgac ccaaccgcag gcatccgact ccttagacag 5700
atcctctccc cccggcatac taaacaaaac ttagggccaa ggaacacaca cactcgacag 5760
aacccagacc ccggcccgcg gcaccgcgcc cccacccccc gaaaaccaga gggagccccc 5820
aaccaaaccc gccggccccc ccggtgccca caggtaggca caccaacccc cgaccagacc 5880
cagcacccag ccaccgacaa tccaagacgg ggggcccccc ccaaaaaaag gcccccaggg 5940
gccgacagcc agcatcgcga ggaagcacac ccaccccaca cacgaccacg gcaaccgaac 6000
cagagtccag accaccctgg gccaccagct cccagactcg gccatcaccc cgcaaaaagg 6060
aaaggccaca acccgcgcac cccagccccg atccggcggg cagccactca acccgaacca 6120
gcacccaaga gcgatccctg ggggaccccc aaaccgcaaa agacatcagt atcccacagc 6180
ctctccaagt cccccggtct cctcctcttc tcgaagggac caaaagatca atccaccaca 6240
tccgacgaca ctcaattccc cacccccaaa ggagacaccg ggaatcccag aatcaagact 6300
catccagtgt ccatcatggg tctcaaggtg aacgtctctg ccatattcat ggcagtactg 6360
ttaactctcc aaacacccac cggtcaaatc cattggggca atctctctaa gataggggtg 6420
gtagggatag gaagtgcaag ctacaaagtt atgactcgtt ccagccatca atcattggtc 6480
ataaaattaa tgcccaatat aactctcctc aataactgca cgagggtaga gatcgcagaa 6540
tacaggagac tactgagaac agttttggaa ccaattagag atgcacttaa tgcaatgacc 6600
cagaatataa gaccggttca gagtgtagct tcaagtagga gacacaagag atttgcggga 6660
gttgtcctgg caggtgcggc cctaggcgtt gccacagctg ctcagataac agccggcatt 6720
gcacttcacc agtccatgct gaactctcaa gccatcgaca atctgagagc aagcctggaa 6780
actactaatc aggcaattga ggcaatcaga caagcagggc aggagatgat attggctgtt 6840
cagggtgtcc aagactacat caataatgag ctgataccgt ctatgaacca actatcttgt 6900
gatttaatcg gccagaagct agggctcaaa ttgctcagat actatacaga aatcctgtca 6960
ttatttggcc ccagcttacg ggaccccata tctgcggaga tatccatcca ggctttgagc 7020
tatgcgcttg gaggagatat caataaggta ttagaaaagc tcggatacag tggaggtgat 7080
ttactgggca tcttagagag cagaggaata aaggcccgga taactcacgt cgacacagag 7140
tcctacttca ttgtactcag tatagcctat ccgacgctgt ccgagattaa gggggtgatt 7200
gtccaccggc tagagggggt ctcgtacaat ataggctctc aagagtggta taccactgtg 7260
cccaagtatg ttgcaaccca agggtacctt atctcgaatt ttgatgagtc atcgtgtact 7320
ttcatgccag aggggactgt gtgcagccaa aatgccttgt acccgatgag tcctctgctc 7380
caagaatgcc tccgggggtc caccaagtcc tgtgctcgta cactcgtatc cgggtctttt 7440
gggaaccggt tcattttatc acaagggaac ctaatagcca attgtgcatc aatcctctgc 7500
aagtgttaca caacaggaac gatcattaat caagaccctg acaagatcct aacatacatt 7560
gctgccgatc actgcccggt ggtcgaggtg aacggtgtga ccatccaagt cgggagcagg 7620
aggtatccgg acgcggtgta cctgcacaga attgacctcg gtcctcccat atcattggag 7680
aggttggacg tagggacaaa tctggggaat gcaattgcta agttggagga tgccaaggaa 7740
ttgttggagt catcggacca gatattgagg agtatgaaag gtttatcgag cactagcata 7800
gtttacatcc tgattgcagt gtgtcttgga gggttgatag ggatccccgc tttaatatgt 7860
tgctgcaggg ggcgctgtaa caaaaaggga gaacaagttg gtatgtcaag accaggccta 7920
aagcctgatc ttacagggac atcaaaatcc tatgtaaggt cgctctgatc ctctacaact 7980
cttgaaacac agatttccca caagtctcct cttcgtcatc aagcaaccac cgcatccagc 8040
atcaagccca cctgaaattg tctccggctt ccctctggcc gaacgatatc ggtagttaat 8100
taaaacttag ggtgcaagat catccacaat gtcaccacaa cgagaccgaa taaatgcctt 8160
ctacaaagac aacccacatc ctaagggaag taggatagtt attaacagag aacatcttat 8220
gattgataga ccttatgttt tgctggctgt tctattcgtc atgtttctga gcttgatcgg 8280
gttgctagcc attgcaggca ttagactcca tcgtgcagcc atctacaccg cagagatcca 8340
taagagcctc agcaccaatc tagatgtaac taactcgatc gagcatcagg tcaaggacgt 8400
gctgacacca ctcttcaaga tcattggtga tgaagtgggc ctgaggacac ctcagagatt 8460
cactgaccta gtgaaattca tctctgacaa aattaaattc cttaatccgg atagggagta 8520
cgacttcaga gatctcactt ggtgtatcaa cccgccagag agaatcaaat tggattatga 8580
tcaatactgt gcagatgtgg ctgctgaaga actcatgaat gcattggtga actcaactct 8640
actggaggcc agggcaacca atcagttcct agctgtctca aagggaaact gctcagggcc 8700
cactacaatc agaggtcaat tctcaaacat gtcgctgtcc ctgttggact tgtatttaag 8760
tcgaggttac aatgtgtcat ctatagtcac tatgacatcc cagggaatgt acgggggaac 8820
ttacctagtg ggaaagccta atctgagcag taaagggtca gagttgtcac aactgagcat 8880
gcaccgagtg tttgaagtag gggttatcag aaatccgggt ttgggggctc cggtgttcca 8940
tatgacaaac tattttgagc aaccagtcag taatgatttc agcaactgca tggtggcttt 9000
gggggagctt aaattcgcag ccctctgtca cagggaagat tctatcacaa ttccctatca 9060
ggggtcaggg aaaggtgtca gcttccagct cgtcaagcta ggtgtctgga aatccccaac 9120
cgacatgcga tcctgggtcc ccctatcaac ggatgatcca gtgatagata ggctttatct 9180
ctcatctcac agaggtgtta tcgctgacaa tcaagcaaaa tgggctgtcc cgacaacacg 9240
gacagatgac aagttgcgaa tggagacatg cttccagcag gcgtgtaagg gtaaaaacca 9300
agcactctgc gagaatcccg agtgggcacc attgaaggat aacaggattc cttcatacgg 9360
ggtcttgtct gttaatctga gtctgacagt tgagcttaaa atcaaaattg cttcaggatt 9420
cgggccattg atcacacacg gttcagggat ggacctatac aaaaccaacc acaacaatgt 9480
gtattggctg actatcccgc caatgaagaa cctagcctta ggtgtaatca acacattgga 9540
gtggataccg agattcaagg ttagtcccaa cctcttcact gttccaatca aggaagcagg 9600
cgaggactgc catgccccaa catacctacc tgcggaggtg gatggtgatg tcaaactcag 9660
ttccaatctg gtaattctac ctggtcagga tctccaatat gttttggcaa cctacgatac 9720
ttccagggtt gaacatgctg tggtttatta tgtttacagc ccaagccgct cattttctta 9780
cttttatcct tttaggttgc ctataaaggg ggtcccaatc gaattacaag tggaatgctt 9840
cacatgggac aaaaaactct ggtgccgtca cttctgtgtg cttgcggact cagaatctgg 9900
tggacatatc actcactctg ggatggtggg catgggagtc agctgcacag tcactcggga 9960
agatggaacc aatcgcagat agggctgcca gtgaaccgat cacatgatgt cacccagaca 10020
tcaggcatac ccactagtgt gaaatagaca tcagaattaa gaaaaacgta gggtccaagt 10080
ggtttcccgt tatggactcg ctatctgtca accagatctt ataccctgaa gttcacctag 10140
atagcccgat agttaccaat aagatagtag ctatcctgga gtatgctcga gtccctcacg 10200
cttacagcct ggaggaccct acactgtgtc agaacatcaa gcaccgccta aaaaacggat 10260
tctccaacca aatgattata aacaatgtgg aagttgggaa tgtcatcaag tccaagctta 10320
ggagttatcc ggcccactct catattccat atccaaattg taatcaggat ttatttaaca 10380
tagaagacaa agagtcaaca aggaagatcc gtgagctcct aaaaaaggga aattcgctgt 10440
actccaaagt cagtgataag gttttccaat gcctgaggga cactaactca cggcttggcc 10500
taggctccga attgagggag gacatcaagg agaaaattat taacttggga gtttacatgc 10560
acagctccca atggtttgag ccctttctgt tttggtttac agtcaagact gagatgaggt 10620
cagtgattaa atcacaaacc catacttgcc ataggaggag acacacacct gtattcttca 10680
ctggtagttc agttgagctg ttaatctctc gtgaccttgt tgctataatc agtaaggaat 10740
ctcaacatgt atattacctg acgtttgaac tggttttgat gtattgtgat gtcatagagg 10800
ggaggttaat gacagagacc gctatgacca ttgatgctag gtatgcagaa cttctaggaa 10860
gagtcagata catgtggaaa ctgatagatg gtttcttccc tgcactcggg aatccaactt 10920
atcaaattgt agccatgctg gagccacttt cacttgctta cctgcaactg agggatataa 10980
cagtagaact cagaggtgct ttccttaacc actgctttac tgaaatacat gatgttcttg 11040
accaaaacgg gttttctgat gaaggtactt atcatgagtt aattgaagcc ctagattaca 11100
ttttcataac tgatgacata catctgacag gggagatttt ctcatttttc agaagtttcg 11160
gccaccccag acttgaagca gtaacggctg ctgaaaatgt caggaaatac atgaatcagc 11220
ctaaagtcat tgtgtatgag actctgatga aaggtcatgc catattttgt ggaatcataa 11280
tcaacggcta tcgtgacagg cacggaggca gttggccacc cctgaccctc cccctgcatg 11340
ctgcagacac aatccggaat gctcaagctt caggtgaagg gttaacacat gagcagtgcg 11400
ttgataactg gaaatcattt gctggagtga gatttggctg ttttatgcct cttagcctgg 11460
acagtgatct gacaatgtac ctaaaggaca aggcacttgc tgctctccaa agggaatggg 11520
attcagttta cccgaaagag ttcctgcgtt acgatcctcc caagggaacc gggtcacgga 11580
ggcttgtaga tgttttcctt aatgattcga gctttgaccc atatgatatg ataatgtatg 11640
tcgtaagtgg agcctacctc catgaccctg agttcaacct gtcttacagc ctgaaagaaa 11700
aggagatcaa ggaaacaggt agacttttcg ctaaaatgac ttacaaaatg agggcatgcc 11760
aagtgatcgc tgaaaatcta atctcaaacg ggattggcaa gtattttaag gacaatggga 11820
tggccaagga tgagcacgat ttgactaagg cactccacac tctggctgtc tcaggagtcc 11880
ccaaagatct caaagaaagt cacagggggg ggccagtctt aaaaacctac tcccgaagcc 11940
cagtccacac aagtaccagg aacgttaaag cagaaaaagg gtttgtagga ttccctcatg 12000
taattcggca gaatcaagac actgatcatc cggagaatat agaaacctac gagacagtca 12060
gcgcatttat cacgactgat ctcaagaagt actgccttaa ttggagatat gagaccatca 12120
gcttatttgc acagaggcta aatgagattt acggattacc ctcatttttt cagtggctgc 12180
ataagaggct tgaaacctct gtcctctatg taagtgaccc tcattgcccc cccgaccttg 12240
acgcccatgt cccgttatgc aaagtcccca atgaccaaat cttcatcaag taccctatgg 12300
gaggtataga agggtattgt cagaagctgt ggaccatcag caccattccc tacttatacc 12360
tggctgctta tgagagcggg gtaaggattg cttcgttagt gcaaggggac aatcagacca 12420
tagccgtaac aaaaagggta cccagcacat ggccttacaa ccttaagaaa cgggaagctg 12480
ctagagtaac tagagattac tttgtaattc ttaggcaaag gctacatgac attggccatc 12540
acctcaaggc aaatgagaca attgtttcat cacatttttt tgtctattca aaaggaatat 12600
attatgatgg gctacttgtg tcccaatcac tcaagagcat cgcaagatgt gtattctggt 12660
cagagactat agttgatgaa acaagggcgg catgcagtaa tattgctaca acaatggcca 12720
aaagcattga gagaggttat gaccgttatc ttgcatattc cctgaacgtc ctaaaagtga 12780
tacagcaaat tttgatctcc cttggcttca caatcaattc aaccatgacc cgggatgtag 12840
tcatacccct cctcacaaac aacgatctct taataaggat ggcactgttg cccgctccta 12900
ttgggggtat gaattatctg aatatgagca ggctgtttgt cagaaacatc ggtgatccag 12960
taacatcatc aattgctgat ctcaagagaa tgattctcgc atcactaatg cctgaagaga 13020
ccctccatca agtaatgaca caacaaccgg gggactcttc attcctagac tgggctagcg 13080
acccttactc agcaaatctt gtatgcgtcc agagcatcac tagactcctc aagaacataa 13140
ctgcaaggtt tgtcctgatc cacagtccaa acccaatgtt aaaagggtta ttccatgatg 13200
acagcaaaga agaggacgag agactggcgg cattcctcat ggacaggcat attatagtac 13260
ctagggcagc tcatgaaatc ctggatcata gtgtcacagg ggcaagagag tctattgcag 13320
gcatgctaga taccacaaaa ggcctgattc gagccagcat gaggaagggg gggttaacct 13380
ctcgagtgat aaccagattg tccaattatg actatgaaca atttagagca gggatggtgc 13440
tattgacagg aagaaagaga aatgtcctca ttgacaaaga gtcatgttca gtgcagctgg 13500
ctagagccct aagaagccat atgtgggcaa gactagctcg aggacggcct atttacggcc 13560
ttgaggtccc tgatgtacta gaatctatgc gaggccacct tattcggcgt catgagacat 13620
gtgtcatctg cgagtgtgga tcagtcaact acggatggtt ttttgtcccc tcgggttgcc 13680
aactggatga tattgacaag gaaacatcat ccttgagagt cccatatatt ggttctacca 13740
ctgatgagag aacagacatg aagctcgcct tcgtaagagc cccaagtaga tccttgcgat 13800
ctgccgttag aatagcaaca gtgtactcat gggcttacgg tgatgatgat agctcttgga 13860
acgaagcctg gttgttggca aggcaaaggg ccaatgtgag cctggaggag ctaagggtga 13920
tcactcccat ctcgacttcg actaatttag cgcataggtt gagggatcgt agcactcaag 13980
tgaaatactc aggtacatcc cttgtccgag tggcaaggta taccacaatc tccaacgaca 14040
atctctcatt tgtcatatca gataagaagg ttgatactaa ctttatatac caacaaggaa 14100
tgcttctagg gttgggtgtt ttagaaacat tgtttcgact cgagaaagat actggatcat 14160
ctaacacggt attacatctt cacgtcgaaa cagattgttg cgtgatcccg atgatagatc 14220
atcccaggat acccagctcc cgcaagctag agctgagggc agagctatgt accaacccat 14280
tgatatatga taatgcacct ttaattgaca gagatgcaac aaggctatac acccagagcc 14340
ataggaggca ccttgtggaa tttgttacat ggtccacacc ccaactatat cacattctag 14400
ctaagtccac agcactatct atgattgacc tggtaacaaa atttgagaag gaccatatga 14460
atgaaatttc agctctcata ggggatgacg atatcaatag tttcataact gagtttctgc 14520
ttatagagcc aagattattc accatctact tgggccagtg tgcagccatc aattgggcat 14580
ttgatgtaca ttatcataga ccatcaggga aatatcagat gggtgagctg ttgtcttcgt 14640
tcctttctag aatgagcaaa ggagtgttta aggtgcttgt caatgctcta agccacccaa 14700
agatctacaa gaaattctgg cattgtggta ttatagagcc tatccatggt ccttcacttg 14760
atgctcaaaa cttgcacaca actgtgtgca acatggttta cacatgctat atgacctacc 14820
tcgacctgtt gttgaatgaa gagttagaag agttcacatt tcttttgtgt gaaagcgatg 14880
aggatgtagt accggacaga ttcgacaaca tccaggcaaa acacttgtgt gttctggcag 14940
atttgtactg tcaaccaggg acctgcccac cgattcgagg tctaaggccg gtagagaaat 15000
gtgcagttct aaccgatcat atcaaggcag aggctaggtt atctccagca ggatcttcgt 15060
ggaacataaa tccaattatt gtagaccatt actcatgctc tctgacttat ctccgtcgag 15120
gatctatcaa acagataaga ttgagagttg atccaggatt catttttgac gccctcgctg 15180
aggtaaatgt cagtcagcca aaggtcggca gcgacgtgag ccatactctt tatgcccccg 15240
gtggatatga cattatggga tatctgaggc agatcaggaa ccggccaaac ccgcaggtgg 15300
aactgggccc cgtggataca tcctgcgcct tgattctttg tgacctgaaa cagaaagaca 15360
ccccgatagt ttacgcgagt gaagccttcc tctacatgac aggttacagc aacgcagagg 15420
tgctgggccg gaattgccgg tttctgcaaa gccctgacgg catggtgaag cccaagagca 15480
cccggaagta cgtggatagt aacacaatca atactatgcg caaggcaatc gacaggaatg 15540
ccgaggtgca ggttgaagta gtcaatttta aaaagaatgg acagcgattt gttaatttcc 15600
tgactatgat acctgttagg gacgaaacag gcgagtatcg atactctatg ggattccagt 15660
gcgaaacaga aggatccacg gggaatagcg ctgatggtgg aggcggtagc ggtggttctg 15720
gtgggagtgg cggaggatca acacaaggcc ggaccggcgg actgaagggc tcttcaggca 15780
gcagcgagaa cctctacttt cagagcggct caagcgggag ttctgggagt ggacggaccg 15840
gcactggcaa tagcgcagat ggcggaggag gatctggcgg gagcggaggg agtggcggcg 15900
ggtctacaca gggaggccgg ccaggcctga aaggcagctc cggttcctct gagaacctgt 15960
acttccagtc tggcagttct ggctcctcag gaagcggacc tgcaggcgga tccatgcata 16020
ctctttatgc ccccggtgga tatgacatta tgggatatct ggaccagatc ggcaaccggc 16080
caaacccgca ggtggaactg ggccccgtgg atacatcctg cgccttgatt ctttgtgacc 16140
tgaaacagaa agacaccccg atagtttacg cgagtgaagc cttcctctac atgacaggtt 16200
acagcaacgc agaggtgctg ggccggaatt gccggtttct gcaaagccct gacggcatgg 16260
tgaagcccaa gagcacccgg aagtacgtgg atagtaacac aatcaatact atccgcaagg 16320
caatcgacag gaatgccgag gtgcaggttg aagtagtcaa ttttaaaaag aatggacagc 16380
gatttgttaa tttcctgact atcatacctg ttagggacga aacaggcgag tatcgatact 16440
ctatgggatt ccagtgcgaa acagaatatc cgtacgatgt cccagattac gcgggcatct 16500
caaatatgag catcaaggat ttcagacctc cacacgatga tgttgcaaaa ttgctcaaag 16560
atatcaacac aagcaagcac aatcttccca tttcaggggg tagtctcgcc aattatgaaa 16620
tccatgcttt ccgcagaatc gggttaaact catctgcttg ctacaaagct gttgagatat 16680
caacattaat taggagatgc cttgagccag gggaagacgg cttgttcttg ggtgaggggt 16740
cgggttctat gttgatcact tataaggaga tactaaaact aaacaagtgc ttctataata 16800
gtggggtttc cgccaattct agatctggtc aaagggaatt agcaccctat ccctccgaag 16860
ttggccttgt cgaacacaga atgggagtag gtaatattgt caaggtgctc tttaacggga 16920
ggcccgaagt cacgtgggta ggcagtatag attgcttcaa tttcatagtc agtaatatcc 16980
ctacctctag tgtggggttt atccattcag atatagagac cttacctaac aaagatacta 17040
tagagaagct agaggaattg gcagccatct tatcgatggc tctactcctt ggcaaaatag 17100
gatcaatact ggtgattaag cttatgcctt tcagcgggga ttttgttcag ggatttataa 17160
gctatgtagg gtctcattat agagaagtga accttgtcta ccctaggtac agcaacttca 17220
tatctactga atcttattta gttatgacag atctcaaagc taaccggcta atgaatcctg 17280
aaaagatcaa gcagcagata attgaatcat ctgtacggac ttcacctgga cttataggtc 17340
acatcctatc cattaagcaa ctaagctgca tacaagcaat tgtgggaggc gcggttagta 17400
gaggtgatat caaccctatt ctgaaaaaac ttacacctat agagcaggtg ctgatcagtt 17460
gcgggttggc aattaacgga cctaaactgt gcaaagaatt aatccaccat gatgttgcct 17520
cagggcaaga tggattgctt aactctatac tcatcctcta cagggagttg gcaagattca 17580
aagacaacca aagaagtcaa caagggatgt tccacgctta ccccgtattg gtaagtagta 17640
ggcaacgaga acttgtatct aggatcactc gcaaattttg ggggcatatt cttctttact 17700
ccgggaacag aaagttgata aatcggttta tccagaatct caagtccggt tatctagtac 17760
tagacttaca ccagaatatc ttcgttaaga atctatccaa gtcagagaaa cagattatta 17820
tgacgggggg tttaaaacgt gagtgggttt ttaaggtaac agtcaaggag accaaagaat 17880
ggtacaagtt agtcggatac agcgctctga ttaaggatta attggttgaa ctccggaacc 17940
ctaatcctgc cctaggtagt taggcattat ttgcaatata ttaaagaaaa ctttgaaaat 18000
acgaagtttc tattcccagc tttgtctggt ggccggcatg gtcccagcct cctcgctggc 18060
gccggctggg caacattccg aggggaccgt cccctcggta atggcgaatg ggacgcggcc 18120
gccgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 18180
acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 18240
caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 18300
cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 18360
cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 18420
tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 18480
cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 18540
gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 18600
aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 18660
gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 18720
caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct 18780
atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 18840
taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 18900
cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 18960
aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 19020
aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 19080
tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 19140
ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 19200
catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 19260
aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 19320
ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 19380
gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 19440
aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 19500
gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 19560
gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 19620
gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 19680
gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 19740
gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 19800
atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 19860
ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 19920
ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 19980
ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 20040
ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 20100
ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 20160
tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 20220
tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 20280
tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 20340
tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 20400
gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 20460
tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 20520
ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 20580
gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 20640
gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 20700
cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 20760
ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 20820
cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 20880
ggaaacagct atgaccatga ttacgccaag cgcgccagtg aattgtaata cgactcacta 20940
ta 20942
<210> 82
<211> 20600
<212> DNA
<213> Artificial Sequence
<220>
<223> p(+)MV323-pMag-direct-nMaghigh
<400> 82
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
acgagatggc cacacttttg aggagcttag cattgttcaa aagaaacaag gacaaaccac 1020
ccattacatc aggatccggt ggagccatca gaggaatcaa acacattatt atagtaccaa 1080
ttcctggaga ttcctcaatt accactcgat ccagactact ggaccggttg gtcaggttaa 1140
ttggaaaccc ggatgtgagc gggcccaaac taacaggggc actaataggt atattatcct 1200
tatttgtgga gtctccaggt caattgattc agaggatcac cgatgaccct gacgttagca 1260
tcaggctgtt agaggttgtt cagagtgacc agtcacaatc tggccttacc ttcgcatcaa 1320
gaggtaccaa catggaggat gaggcggacc aatacttttc acatgatgat ccaagcagta 1380
gtgatcaatc caggtccgga tggttcgaga acaaggaaat ctcagatatt gaagtgcaag 1440
accctgaggg attcaacatg attctgggta ccattctagc ccagatctgg gtcttgctcg 1500
caaaggcggt tacggcccca gacacggcag ctgattcgga gctaagaagg tggataaagt 1560
acacccaaca aagaagggta gttggtgaat ttagattgga gagaaaatgg ttggatgtgg 1620
tgaggaacag gattgccgag gacctctctt tacgccgatt catggtggct ctaatcctgg 1680
atatcaagag gacacccggg aacaaaccta ggattgctga aatgatatgt gacattgata 1740
catatatcgt agaggcagga ttagccagtt ttatcctgac tattaagttt gggatagaaa 1800
ctatgtatcc tgctcttgga ctgcatgaat ttgctggtga gttatccaca cttgagtcct 1860
tgatgaatct ttaccagcaa atgggagaaa ctgcacccta catggtaatc ctagagaact 1920
caattcagaa caagttcagt gcaggatcat accctctgct ctggagctat gccatgggag 1980
taggagtgga acttgaaaac tccatgggag gtttgaactt tggtcgatct tactttgatc 2040
cagcatattt tagattaggg caagagatgg tgaggaggtc agctggaaag gtcagttcca 2100
cattggcatc cgaactcggt atcactgccg aggatgcaag gcttgtttca gagattgcaa 2160
tgcatactac tgaggacagg atcagtagag cggtcggacc cagacaagcc caagtgtcat 2220
ttctacacgg tgatcaaagt gagaatgagc taccaggatt ggggggcaag gaagatagga 2280
gggtcaaaca gggtcgggga gaagccaggg agagctacag agaaaccggg tccagcagag 2340
caagtgatgc gagagctgcc catcctccaa ccagcatgcc cctagacatt gacactgcat 2400
cggagtcagg ccaagatccg caggacagtc gaaggtcagc tgacgccctg ctcaggctgc 2460
aagccatggc aggaatcttg gaagaacaag gctcagacac ggacacccct agggtataca 2520
atgacagaga tcttctagac taggtgcgag aggccgagga ccagaacaac atccgcctac 2580
cctccatcat tgttataaaa aacttaggaa ccaggtccac acagccgcca gccaaccaac 2640
catccactcc cacgactgga gccgatggca gaagagcagg cacgccatgt caaaaacgga 2700
ctggaatgca tccgggctct caaggccgag cccatcggct cactggccgt cgaggaagcc 2760
atggcagcat ggtcagaaat atcagacaac ccaggacagg accgagccac ctgcaaggaa 2820
gaggaggcag gcagttcggg tctcagcaaa ccatgcctct cagcaattgg atcaactgaa 2880
ggcggtgcac ctcgcatccg cggtcaggga tctggagaaa gcgatgacga cgctgaaact 2940
ttgggaatcc cctcaagaaa tctccaggca tcaagcactg ggttacagtg ttatcatgtt 3000
tatgatcaca gcggtgaagc ggttaaggga atccaagatg ctgactctat catggttcaa 3060
tcaggccttg atggtgatag caccctctca ggaggagacg atgaatctga aaacagcgat 3120
gtggatattg gcgaacctga taccgaggga tatgctatca ctgaccgggg atctgctccc 3180
atctctatgg ggttcagggc ttctgatgtt gaaactgcag aaggagggga gatccacgag 3240
ctcctgaaac tccaatccag aggcaacaac tttccgaagc ttgggaaaac tctcaatgtt 3300
cctccgcccc cgaaccccag tagggccagc acttccgaga cacccattaa aaagggcaca 3360
gacgcgagat tggcctcatt tggaacggag atcgcgtctt tattgacagg tggtgcaacc 3420
caatgtgctc gaaagtcacc ctcggaacca tcagggccag gtgcacctgc ggggaatgtc 3480
cccgagtgtg tgagcaatgc cgcactgata caggagtgga cacccgaatc tggtaccaca 3540
atctccccga gatcccagaa taatgaagaa gggggagact attatgatga tgagctgttc 3600
tccgatgtcc aagacatcaa aacagccttg gccaaaatac acgaggataa tcagaagata 3660
atctccaagc tagaatcatt gctgttattg aagggagaag ttgagtcaat taagaagcag 3720
atcaacaggc aaaatatcag catatccacc ctggaaggac acctctcaag catcatgatt 3780
gccattcctg gacttgggaa ggatcccaac gaccccactg cagatgtcga actcaatccc 3840
gacctgaaac ccatcatagg cagagattca ggccgagcac tggccgaagt tctcaagaag 3900
cccgttgcca gccgacaact ccagggaatg actaatggac ggaccagttc cagaggacag 3960
ctgctgaagg aatttcaact aaagccgatc gggaaaaagg tgagctcagc cgtcgggttt 4020
gttcctgaca ccggccctgc atcacgcagt gtaatccgct ccattataaa atccagccgg 4080
ctagaggagg atcggaagcg ttacctgatg actctccttg atgatatcaa aggagccaac 4140
gatcttgcca agttccacca gatgctgatg aagataataa tgaagtagct acagctcaac 4200
ttacctgcca accccatgcc agtcgaccta attagtacaa cctaaatcca ttataaaaaa 4260
cttaggagca aagtgattgc ctcctaagtt ccacaatgac agagatctac gatttcgaca 4320
agtcggcatg ggacatcaaa gggtcgatcg ctccgataca acctaccacc tacagtgatg 4380
gcaggctggt gccccaggtc agagtcatag atcctggtct aggtgatagg aaggatgaat 4440
gctttatgta catgtttctg ctgggggttg ttgaggacag cgatccccta gggcctccaa 4500
tcgggcgagc attcgggtcc ctgcccttag gtgttggtag atccacagca aaacccgagg 4560
aactcctcaa agaggccact gagcttgaca tagttgttag acgtacagca gggctcaatg 4620
aaaaactggt gttctacaac aacaccccac taaccctcct cacaccttgg agaaaggtcc 4680
taacaacagg gagtgtcttc aatgcaaacc aagtgtgcaa tgcggttaat ctaataccgc 4740
tggacacccc gcagaggttc cgtgttgttt atatgagcat cacccgtctt tcggataacg 4800
ggtattacac cgttcccaga agaatgctgg aattcagatc ggtcaatgca gtggccttca 4860
acctgctagt gacccttagg attgacaagg cgattggccc tgggaagatc atcgacaatg 4920
cagagcaact tcctgaggca acatttatgg tccacatcgg gaacttcagg agaaagaaga 4980
gtgaagtcta ctctgccgat tattgcaaaa tgaaaatcga aaagatgggc ctggtttttg 5040
cacttggtgg gatagggggc accagtcttc acattagaag cacaggcaaa atgagcaaga 5100
ctctccatgc acaactcggg ttcaagaaga ccttatgtta cccactgatg gatatcaatg 5160
aagaccttaa tcggttactc tggaggagca gatgcaagat agtaagaatc caggcagttt 5220
tgcagccatc agttcctcaa gaattccgca tttacgacga cgtgatcata aatgatgacc 5280
aaggactatt caaagttctg tagaccgcag tgcccagcaa tacccgaaaa cgacccccct 5340
cataatgaca gccagaaggc ccggacaaaa aagccccctc cagaagactc cacggaccaa 5400
gcgagaggcc agccagcagc cgacagcaag tgtggacacc aggcggccca agcacagaac 5460
agccccgaca caaggccacc accagccatc ccaatctgcg tcctcctcgt gggacccccg 5520
aggaccaacc ccgaaggtcg ctccgaacac agaccaccaa ccgcatcccc acagctcccg 5580
ggaaaggaac ccccagcaac tggaaggccc ctccccccct cccccaacgc aagaacccca 5640
caaccgaacc gcacaagcga ccgaggtgac ccaaccgcag gcatccgact ccttagacag 5700
atcctctccc cccggcatac taaacaaaac ttagggccaa ggaacacaca cactcgacag 5760
aacccagacc ccggcccgcg gcaccgcgcc cccacccccc gaaaaccaga gggagccccc 5820
aaccaaaccc gccggccccc ccggtgccca caggtaggca caccaacccc cgaccagacc 5880
cagcacccag ccaccgacaa tccaagacgg ggggcccccc ccaaaaaaag gcccccaggg 5940
gccgacagcc agcatcgcga ggaagcacac ccaccccaca cacgaccacg gcaaccgaac 6000
cagagtccag accaccctgg gccaccagct cccagactcg gccatcaccc cgcaaaaagg 6060
aaaggccaca acccgcgcac cccagccccg atccggcggg cagccactca acccgaacca 6120
gcacccaaga gcgatccctg ggggaccccc aaaccgcaaa agacatcagt atcccacagc 6180
ctctccaagt cccccggtct cctcctcttc tcgaagggac caaaagatca atccaccaca 6240
tccgacgaca ctcaattccc cacccccaaa ggagacaccg ggaatcccag aatcaagact 6300
catccagtgt ccatcatggg tctcaaggtg aacgtctctg ccatattcat ggcagtactg 6360
ttaactctcc aaacacccac cggtcaaatc cattggggca atctctctaa gataggggtg 6420
gtagggatag gaagtgcaag ctacaaagtt atgactcgtt ccagccatca atcattggtc 6480
ataaaattaa tgcccaatat aactctcctc aataactgca cgagggtaga gatcgcagaa 6540
tacaggagac tactgagaac agttttggaa ccaattagag atgcacttaa tgcaatgacc 6600
cagaatataa gaccggttca gagtgtagct tcaagtagga gacacaagag atttgcggga 6660
gttgtcctgg caggtgcggc cctaggcgtt gccacagctg ctcagataac agccggcatt 6720
gcacttcacc agtccatgct gaactctcaa gccatcgaca atctgagagc aagcctggaa 6780
actactaatc aggcaattga ggcaatcaga caagcagggc aggagatgat attggctgtt 6840
cagggtgtcc aagactacat caataatgag ctgataccgt ctatgaacca actatcttgt 6900
gatttaatcg gccagaagct agggctcaaa ttgctcagat actatacaga aatcctgtca 6960
ttatttggcc ccagcttacg ggaccccata tctgcggaga tatccatcca ggctttgagc 7020
tatgcgcttg gaggagatat caataaggta ttagaaaagc tcggatacag tggaggtgat 7080
ttactgggca tcttagagag cagaggaata aaggcccgga taactcacgt cgacacagag 7140
tcctacttca ttgtactcag tatagcctat ccgacgctgt ccgagattaa gggggtgatt 7200
gtccaccggc tagagggggt ctcgtacaat ataggctctc aagagtggta taccactgtg 7260
cccaagtatg ttgcaaccca agggtacctt atctcgaatt ttgatgagtc atcgtgtact 7320
ttcatgccag aggggactgt gtgcagccaa aatgccttgt acccgatgag tcctctgctc 7380
caagaatgcc tccgggggtc caccaagtcc tgtgctcgta cactcgtatc cgggtctttt 7440
gggaaccggt tcattttatc acaagggaac ctaatagcca attgtgcatc aatcctctgc 7500
aagtgttaca caacaggaac gatcattaat caagaccctg acaagatcct aacatacatt 7560
gctgccgatc actgcccggt ggtcgaggtg aacggtgtga ccatccaagt cgggagcagg 7620
aggtatccgg acgcggtgta cctgcacaga attgacctcg gtcctcccat atcattggag 7680
aggttggacg tagggacaaa tctggggaat gcaattgcta agttggagga tgccaaggaa 7740
ttgttggagt catcggacca gatattgagg agtatgaaag gtttatcgag cactagcata 7800
gtttacatcc tgattgcagt gtgtcttgga gggttgatag ggatccccgc tttaatatgt 7860
tgctgcaggg ggcgctgtaa caaaaaggga gaacaagttg gtatgtcaag accaggccta 7920
aagcctgatc ttacagggac atcaaaatcc tatgtaaggt cgctctgatc ctctacaact 7980
cttgaaacac agatttccca caagtctcct cttcgtcatc aagcaaccac cgcatccagc 8040
atcaagccca cctgaaattg tctccggctt ccctctggcc gaacgatatc ggtagttaat 8100
taaaacttag ggtgcaagat catccacaat gtcaccacaa cgagaccgaa taaatgcctt 8160
ctacaaagac aacccacatc ctaagggaag taggatagtt attaacagag aacatcttat 8220
gattgataga ccttatgttt tgctggctgt tctattcgtc atgtttctga gcttgatcgg 8280
gttgctagcc attgcaggca ttagactcca tcgtgcagcc atctacaccg cagagatcca 8340
taagagcctc agcaccaatc tagatgtaac taactcgatc gagcatcagg tcaaggacgt 8400
gctgacacca ctcttcaaga tcattggtga tgaagtgggc ctgaggacac ctcagagatt 8460
cactgaccta gtgaaattca tctctgacaa aattaaattc cttaatccgg atagggagta 8520
cgacttcaga gatctcactt ggtgtatcaa cccgccagag agaatcaaat tggattatga 8580
tcaatactgt gcagatgtgg ctgctgaaga actcatgaat gcattggtga actcaactct 8640
actggaggcc agggcaacca atcagttcct agctgtctca aagggaaact gctcagggcc 8700
cactacaatc agaggtcaat tctcaaacat gtcgctgtcc ctgttggact tgtatttaag 8760
tcgaggttac aatgtgtcat ctatagtcac tatgacatcc cagggaatgt acgggggaac 8820
ttacctagtg ggaaagccta atctgagcag taaagggtca gagttgtcac aactgagcat 8880
gcaccgagtg tttgaagtag gggttatcag aaatccgggt ttgggggctc cggtgttcca 8940
tatgacaaac tattttgagc aaccagtcag taatgatttc agcaactgca tggtggcttt 9000
gggggagctt aaattcgcag ccctctgtca cagggaagat tctatcacaa ttccctatca 9060
ggggtcaggg aaaggtgtca gcttccagct cgtcaagcta ggtgtctgga aatccccaac 9120
cgacatgcga tcctgggtcc ccctatcaac ggatgatcca gtgatagata ggctttatct 9180
ctcatctcac agaggtgtta tcgctgacaa tcaagcaaaa tgggctgtcc cgacaacacg 9240
gacagatgac aagttgcgaa tggagacatg cttccagcag gcgtgtaagg gtaaaaacca 9300
agcactctgc gagaatcccg agtgggcacc attgaaggat aacaggattc cttcatacgg 9360
ggtcttgtct gttaatctga gtctgacagt tgagcttaaa atcaaaattg cttcaggatt 9420
cgggccattg atcacacacg gttcagggat ggacctatac aaaaccaacc acaacaatgt 9480
gtattggctg actatcccgc caatgaagaa cctagcctta ggtgtaatca acacattgga 9540
gtggataccg agattcaagg ttagtcccaa cctcttcact gttccaatca aggaagcagg 9600
cgaggactgc catgccccaa catacctacc tgcggaggtg gatggtgatg tcaaactcag 9660
ttccaatctg gtaattctac ctggtcagga tctccaatat gttttggcaa cctacgatac 9720
ttccagggtt gaacatgctg tggtttatta tgtttacagc ccaagccgct cattttctta 9780
cttttatcct tttaggttgc ctataaaggg ggtcccaatc gaattacaag tggaatgctt 9840
cacatgggac aaaaaactct ggtgccgtca cttctgtgtg cttgcggact cagaatctgg 9900
tggacatatc actcactctg ggatggtggg catgggagtc agctgcacag tcactcggga 9960
agatggaacc aatcgcagat agggctgcca gtgaaccgat cacatgatgt cacccagaca 10020
tcaggcatac ccactagtgt gaaatagaca tcagaattaa gaaaaacgta gggtccaagt 10080
ggtttcccgt tatggactcg ctatctgtca accagatctt ataccctgaa gttcacctag 10140
atagcccgat agttaccaat aagatagtag ctatcctgga gtatgctcga gtccctcacg 10200
cttacagcct ggaggaccct acactgtgtc agaacatcaa gcaccgccta aaaaacggat 10260
tctccaacca aatgattata aacaatgtgg aagttgggaa tgtcatcaag tccaagctta 10320
ggagttatcc ggcccactct catattccat atccaaattg taatcaggat ttatttaaca 10380
tagaagacaa agagtcaaca aggaagatcc gtgagctcct aaaaaaggga aattcgctgt 10440
actccaaagt cagtgataag gttttccaat gcctgaggga cactaactca cggcttggcc 10500
taggctccga attgagggag gacatcaagg agaaaattat taacttggga gtttacatgc 10560
acagctccca atggtttgag ccctttctgt tttggtttac agtcaagact gagatgaggt 10620
cagtgattaa atcacaaacc catacttgcc ataggaggag acacacacct gtattcttca 10680
ctggtagttc agttgagctg ttaatctctc gtgaccttgt tgctataatc agtaaggaat 10740
ctcaacatgt atattacctg acgtttgaac tggttttgat gtattgtgat gtcatagagg 10800
ggaggttaat gacagagacc gctatgacca ttgatgctag gtatgcagaa cttctaggaa 10860
gagtcagata catgtggaaa ctgatagatg gtttcttccc tgcactcggg aatccaactt 10920
atcaaattgt agccatgctg gagccacttt cacttgctta cctgcaactg agggatataa 10980
cagtagaact cagaggtgct ttccttaacc actgctttac tgaaatacat gatgttcttg 11040
accaaaacgg gttttctgat gaaggtactt atcatgagtt aattgaagcc ctagattaca 11100
ttttcataac tgatgacata catctgacag gggagatttt ctcatttttc agaagtttcg 11160
gccaccccag acttgaagca gtaacggctg ctgaaaatgt caggaaatac atgaatcagc 11220
ctaaagtcat tgtgtatgag actctgatga aaggtcatgc catattttgt ggaatcataa 11280
tcaacggcta tcgtgacagg cacggaggca gttggccacc cctgaccctc cccctgcatg 11340
ctgcagacac aatccggaat gctcaagctt caggtgaagg gttaacacat gagcagtgcg 11400
ttgataactg gaaatcattt gctggagtga gatttggctg ttttatgcct cttagcctgg 11460
acagtgatct gacaatgtac ctaaaggaca aggcacttgc tgctctccaa agggaatggg 11520
attcagttta cccgaaagag ttcctgcgtt acgatcctcc caagggaacc gggtcacgga 11580
ggcttgtaga tgttttcctt aatgattcga gctttgaccc atatgatatg ataatgtatg 11640
tcgtaagtgg agcctacctc catgaccctg agttcaacct gtcttacagc ctgaaagaaa 11700
aggagatcaa ggaaacaggt agacttttcg ctaaaatgac ttacaaaatg agggcatgcc 11760
aagtgatcgc tgaaaatcta atctcaaacg ggattggcaa gtattttaag gacaatggga 11820
tggccaagga tgagcacgat ttgactaagg cactccacac tctggctgtc tcaggagtcc 11880
ccaaagatct caaagaaagt cacagggggg ggccagtctt aaaaacctac tcccgaagcc 11940
cagtccacac aagtaccagg aacgttaaag cagaaaaagg gtttgtagga ttccctcatg 12000
taattcggca gaatcaagac actgatcatc cggagaatat agaaacctac gagacagtca 12060
gcgcatttat cacgactgat ctcaagaagt actgccttaa ttggagatat gagaccatca 12120
gcttatttgc acagaggcta aatgagattt acggattacc ctcatttttt cagtggctgc 12180
ataagaggct tgaaacctct gtcctctatg taagtgaccc tcattgcccc cccgaccttg 12240
acgcccatgt cccgttatgc aaagtcccca atgaccaaat cttcatcaag taccctatgg 12300
gaggtataga agggtattgt cagaagctgt ggaccatcag caccattccc tacttatacc 12360
tggctgctta tgagagcggg gtaaggattg cttcgttagt gcaaggggac aatcagacca 12420
tagccgtaac aaaaagggta cccagcacat ggccttacaa ccttaagaaa cgggaagctg 12480
ctagagtaac tagagattac tttgtaattc ttaggcaaag gctacatgac attggccatc 12540
acctcaaggc aaatgagaca attgtttcat cacatttttt tgtctattca aaaggaatat 12600
attatgatgg gctacttgtg tcccaatcac tcaagagcat cgcaagatgt gtattctggt 12660
cagagactat agttgatgaa acaagggcgg catgcagtaa tattgctaca acaatggcca 12720
aaagcattga gagaggttat gaccgttatc ttgcatattc cctgaacgtc ctaaaagtga 12780
tacagcaaat tttgatctcc cttggcttca caatcaattc aaccatgacc cgggatgtag 12840
tcatacccct cctcacaaac aacgatctct taataaggat ggcactgttg cccgctccta 12900
ttgggggtat gaattatctg aatatgagca ggctgtttgt cagaaacatc ggtgatccag 12960
taacatcatc aattgctgat ctcaagagaa tgattctcgc atcactaatg cctgaagaga 13020
ccctccatca agtaatgaca caacaaccgg gggactcttc attcctagac tgggctagcg 13080
acccttactc agcaaatctt gtatgcgtcc agagcatcac tagactcctc aagaacataa 13140
ctgcaaggtt tgtcctgatc cacagtccaa acccaatgtt aaaagggtta ttccatgatg 13200
acagcaaaga agaggacgag agactggcgg cattcctcat ggacaggcat attatagtac 13260
ctagggcagc tcatgaaatc ctggatcata gtgtcacagg ggcaagagag tctattgcag 13320
gcatgctaga taccacaaaa ggcctgattc gagccagcat gaggaagggg gggttaacct 13380
ctcgagtgat aaccagattg tccaattatg actatgaaca atttagagca gggatggtgc 13440
tattgacagg aagaaagaga aatgtcctca ttgacaaaga gtcatgttca gtgcagctgg 13500
ctagagccct aagaagccat atgtgggcaa gactagctcg aggacggcct atttacggcc 13560
ttgaggtccc tgatgtacta gaatctatgc gaggccacct tattcggcgt catgagacat 13620
gtgtcatctg cgagtgtgga tcagtcaact acggatggtt ttttgtcccc tcgggttgcc 13680
aactggatga tattgacaag gaaacatcat ccttgagagt cccatatatt ggttctacca 13740
ctgatgagag aacagacatg aagctcgcct tcgtaagagc cccaagtaga tccttgcgat 13800
ctgccgttag aatagcaaca gtgtactcat gggcttacgg tgatgatgat agctcttgga 13860
acgaagcctg gttgttggca aggcaaaggg ccaatgtgag cctggaggag ctaagggtga 13920
tcactcccat ctcgacttcg actaatttag cgcataggtt gagggatcgt agcactcaag 13980
tgaaatactc aggtacatcc cttgtccgag tggcaaggta taccacaatc tccaacgaca 14040
atctctcatt tgtcatatca gataagaagg ttgatactaa ctttatatac caacaaggaa 14100
tgcttctagg gttgggtgtt ttagaaacat tgtttcgact cgagaaagat actggatcat 14160
ctaacacggt attacatctt cacgtcgaaa cagattgttg cgtgatcccg atgatagatc 14220
atcccaggat acccagctcc cgcaagctag agctgagggc agagctatgt accaacccat 14280
tgatatatga taatgcacct ttaattgaca gagatgcaac aaggctatac acccagagcc 14340
ataggaggca ccttgtggaa tttgttacat ggtccacacc ccaactatat cacattctag 14400
ctaagtccac agcactatct atgattgacc tggtaacaaa atttgagaag gaccatatga 14460
atgaaatttc agctctcata ggggatgacg atatcaatag tttcataact gagtttctgc 14520
ttatagagcc aagattattc accatctact tgggccagtg tgcagccatc aattgggcat 14580
ttgatgtaca ttatcataga ccatcaggga aatatcagat gggtgagctg ttgtcttcgt 14640
tcctttctag aatgagcaaa ggagtgttta aggtgcttgt caatgctcta agccacccaa 14700
agatctacaa gaaattctgg cattgtggta ttatagagcc tatccatggt ccttcacttg 14760
atgctcaaaa cttgcacaca actgtgtgca acatggttta cacatgctat atgacctacc 14820
tcgacctgtt gttgaatgaa gagttagaag agttcacatt tcttttgtgt gaaagcgatg 14880
aggatgtagt accggacaga ttcgacaaca tccaggcaaa acacttgtgt gttctggcag 14940
atttgtactg tcaaccaggg acctgcccac cgattcgagg tctaaggccg gtagagaaat 15000
gtgcagttct aaccgatcat atcaaggcag aggctaggtt atctccagca ggatcttcgt 15060
ggaacataaa tccaattatt gtagaccatt actcatgctc tctgacttat ctccgtcgag 15120
gatctatcaa acagataaga ttgagagttg atccaggatt catttttgac gccctcgctg 15180
aggtaaatgt cagtcagcca aaggtcggca gcgacgtcag ccatactctt tatgcccccg 15240
gtggatatga cattatggga tatctgaggc agatcaggaa ccggccaaac ccgcaggtgg 15300
aactgggccc cgtggataca tcctgcgcct tgattctttg tgacctgaaa cagaaagaca 15360
ccccgatagt ttacgcgagt gaagccttcc tctacatgac aggttacagc aacgcagagg 15420
tgctgggccg gaattgccgg tttctgcaaa gccctgacgg catggtgaag cccaagagca 15480
cccggaagta cgtggatagt aacacaatca atactatgcg caaggcaatc gacaggaatg 15540
ccgaggtgca ggttgaagta gtcaatttta aaaagaatgg acagcgattt gttaatttcc 15600
tgactatgat acctgttagg gacgaaacag gcgagtatcg atactctatg ggattccagt 15660
gcgaaacaga aatgcacact ctgtatgctc caggtggcta tgacatcatg ggctatctgg 15720
atcagatagg gaatcgtcct aatccccaag tggaacttgg accagtagac actagctgtg 15780
cactgattct ctgtgacctc aagcagaagg acacacccat cgtgtacgcc tctgaagcgt 15840
ttctgtacat gacaggctac agcaatgccg aagtcttggg gagaaattgc cgatttctgc 15900
agagtcctga cggtatggtc aaacccaagt caacgaggaa atacgtcgat tccaacacca 15960
tcaacaccat tcggaaagcc atcgatcgga atgctgaggt gcaagtggag gttgtgaact 16020
tcaagaagaa cggacagagg ttcgtgaact tcctgaccat aattccggtt agagatgaga 16080
caggagagta ccgctattcc atggggttcc agtgcgaaac agaatatccg tacgacgtcc 16140
cagattacgc gggcatctca aatatgagca tcaaggattt cagacctcca cacgatgatg 16200
ttgcaaaatt gctcaaagat atcaacacaa gcaagcacaa tcttcccatt tcagggggta 16260
gtctcgccaa ttatgaaatc catgctttcc gcagaatcgg gttaaactca tctgcttgct 16320
acaaagctgt tgagatatca acattaatta ggagatgcct tgagccaggg gaagacggct 16380
tgttcttggg tgaggggtcg ggttctatgt tgatcactta taaggagata ctaaaactaa 16440
acaagtgctt ctataatagt ggggtttccg ccaattctag atctggtcaa agggaattag 16500
caccctatcc ctccgaagtt ggccttgtcg aacacagaat gggagtaggt aatattgtca 16560
aggtgctctt taacgggagg cccgaagtca cgtgggtagg cagtatagat tgcttcaatt 16620
tcatagtcag taatatccct acctctagtg tggggtttat ccattcagat atagagacct 16680
tacctaacaa agatactata gagaagctag aggaattggc agccatctta tcgatggctc 16740
tactccttgg caaaatagga tcaatactgg tgattaagct tatgcctttc agcggggatt 16800
ttgttcaggg atttataagc tatgtagggt ctcattatag agaagtgaac cttgtctacc 16860
ctaggtacag caacttcata tctactgaat cttatttagt tatgacagat ctcaaagcta 16920
accggctaat gaatcctgaa aagatcaagc agcagataat tgaatcatct gtacggactt 16980
cacctggact tataggtcac atcctatcca ttaagcaact aagctgcata caagcaattg 17040
tgggaggcgc ggttagtaga ggtgatatca accctattct gaaaaaactt acacctatag 17100
agcaggtgct gatcagttgc gggttggcaa ttaacggacc taaactgtgc aaagaattaa 17160
tccaccatga tgttgcctca gggcaagatg gattgcttaa ctctatactc atcctctaca 17220
gggagttggc aagattcaaa gacaaccaaa gaagtcaaca agggatgttc cacgcttacc 17280
ccgtattggt aagtagtagg caacgagaac ttgtatctag gatcactcgc aaattttggg 17340
ggcatattct tctttactcc gggaacagaa agttgataaa tcggtttatc cagaatctca 17400
agtccggtta tctagtacta gacttacacc agaatatctt cgttaagaat ctatccaagt 17460
cagagaaaca gattattatg acggggggtt taaaacgtga gtgggttttt aaggtaacag 17520
tcaaggagac caaagaatgg tacaagttag tcggatacag cgctctgatt aaggattaat 17580
tggttgaact ccggaaccct aatcctgccc taggtagtta ggcattattt gcaatatatt 17640
aaagaaaact ttgaaaatac gaagtttcta ttcccagctt tgtctggtgg ccggcatggt 17700
cccagcctcc tcgctggcgc cggctgggca acattccgag gggaccgtcc cctcggtaat 17760
ggcgaatggg acgcggccgc cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 17820
aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 17880
taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 17940
atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 18000
gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 18060
cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 18120
atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 18180
tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 18240
tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 18300
tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 18360
atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 18420
aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 18480
atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 18540
caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 18600
cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 18660
tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 18720
tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 18780
gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 18840
tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 18900
gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 18960
aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 19020
gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 19080
atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 19140
caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 19200
ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 19260
attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 19320
agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 19380
aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 19440
catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 19500
ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 19560
tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 19620
ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 19680
ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 19740
ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 19800
gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 19860
aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 19920
acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 19980
gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 20040
gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 20100
cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 20160
aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 20220
gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 20280
cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 20340
atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 20400
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 20460
taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 20520
ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgccagtgaa 20580
ttgtaatacg actcactata 20600
<210> 83
<211> 20678
<212> DNA
<213> Artificial Sequence
<220>
<223> p(+)MV323-pMag-linker-nMag
<400> 83
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
acgagatggc cacacttttg aggagcttag cattgttcaa aagaaacaag gacaaaccac 1020
ccattacatc aggatccggt ggagccatca gaggaatcaa acacattatt atagtaccaa 1080
ttcctggaga ttcctcaatt accactcgat ccagactact ggaccggttg gtcaggttaa 1140
ttggaaaccc ggatgtgagc gggcccaaac taacaggggc actaataggt atattatcct 1200
tatttgtgga gtctccaggt caattgattc agaggatcac cgatgaccct gacgttagca 1260
tcaggctgtt agaggttgtt cagagtgacc agtcacaatc tggccttacc ttcgcatcaa 1320
gaggtaccaa catggaggat gaggcggacc aatacttttc acatgatgat ccaagcagta 1380
gtgatcaatc caggtccgga tggttcgaga acaaggaaat ctcagatatt gaagtgcaag 1440
accctgaggg attcaacatg attctgggta ccattctagc ccagatctgg gtcttgctcg 1500
caaaggcggt tacggcccca gacacggcag ctgattcgga gctaagaagg tggataaagt 1560
acacccaaca aagaagggta gttggtgaat ttagattgga gagaaaatgg ttggatgtgg 1620
tgaggaacag gattgccgag gacctctctt tacgccgatt catggtggct ctaatcctgg 1680
atatcaagag gacacccggg aacaaaccta ggattgctga aatgatatgt gacattgata 1740
catatatcgt agaggcagga ttagccagtt ttatcctgac tattaagttt gggatagaaa 1800
ctatgtatcc tgctcttgga ctgcatgaat ttgctggtga gttatccaca cttgagtcct 1860
tgatgaatct ttaccagcaa atgggagaaa ctgcacccta catggtaatc ctagagaact 1920
caattcagaa caagttcagt gcaggatcat accctctgct ctggagctat gccatgggag 1980
taggagtgga acttgaaaac tccatgggag gtttgaactt tggtcgatct tactttgatc 2040
cagcatattt tagattaggg caagagatgg tgaggaggtc agctggaaag gtcagttcca 2100
cattggcatc cgaactcggt atcactgccg aggatgcaag gcttgtttca gagattgcaa 2160
tgcatactac tgaggacagg atcagtagag cggtcggacc cagacaagcc caagtgtcat 2220
ttctacacgg tgatcaaagt gagaatgagc taccaggatt ggggggcaag gaagatagga 2280
gggtcaaaca gggtcgggga gaagccaggg agagctacag agaaaccggg tccagcagag 2340
caagtgatgc gagagctgcc catcctccaa ccagcatgcc cctagacatt gacactgcat 2400
cggagtcagg ccaagatccg caggacagtc gaaggtcagc tgacgccctg ctcaggctgc 2460
aagccatggc aggaatcttg gaagaacaag gctcagacac ggacacccct agggtataca 2520
atgacagaga tcttctagac taggtgcgag aggccgagga ccagaacaac atccgcctac 2580
cctccatcat tgttataaaa aacttaggaa ccaggtccac acagccgcca gccaaccaac 2640
catccactcc cacgactgga gccgatggca gaagagcagg cacgccatgt caaaaacgga 2700
ctggaatgca tccgggctct caaggccgag cccatcggct cactggccgt cgaggaagcc 2760
atggcagcat ggtcagaaat atcagacaac ccaggacagg accgagccac ctgcaaggaa 2820
gaggaggcag gcagttcggg tctcagcaaa ccatgcctct cagcaattgg atcaactgaa 2880
ggcggtgcac ctcgcatccg cggtcaggga tctggagaaa gcgatgacga cgctgaaact 2940
ttgggaatcc cctcaagaaa tctccaggca tcaagcactg ggttacagtg ttatcatgtt 3000
tatgatcaca gcggtgaagc ggttaaggga atccaagatg ctgactctat catggttcaa 3060
tcaggccttg atggtgatag caccctctca ggaggagacg atgaatctga aaacagcgat 3120
gtggatattg gcgaacctga taccgaggga tatgctatca ctgaccgggg atctgctccc 3180
atctctatgg ggttcagggc ttctgatgtt gaaactgcag aaggagggga gatccacgag 3240
ctcctgaaac tccaatccag aggcaacaac tttccgaagc ttgggaaaac tctcaatgtt 3300
cctccgcccc cgaaccccag tagggccagc acttccgaga cacccattaa aaagggcaca 3360
gacgcgagat tggcctcatt tggaacggag atcgcgtctt tattgacagg tggtgcaacc 3420
caatgtgctc gaaagtcacc ctcggaacca tcagggccag gtgcacctgc ggggaatgtc 3480
cccgagtgtg tgagcaatgc cgcactgata caggagtgga cacccgaatc tggtaccaca 3540
atctccccga gatcccagaa taatgaagaa gggggagact attatgatga tgagctgttc 3600
tccgatgtcc aagacatcaa aacagccttg gccaaaatac acgaggataa tcagaagata 3660
atctccaagc tagaatcatt gctgttattg aagggagaag ttgagtcaat taagaagcag 3720
atcaacaggc aaaatatcag catatccacc ctggaaggac acctctcaag catcatgatt 3780
gccattcctg gacttgggaa ggatcccaac gaccccactg cagatgtcga actcaatccc 3840
gacctgaaac ccatcatagg cagagattca ggccgagcac tggccgaagt tctcaagaag 3900
cccgttgcca gccgacaact ccagggaatg actaatggac ggaccagttc cagaggacag 3960
ctgctgaagg aatttcaact aaagccgatc gggaaaaagg tgagctcagc cgtcgggttt 4020
gttcctgaca ccggccctgc atcacgcagt gtaatccgct ccattataaa atccagccgg 4080
ctagaggagg atcggaagcg ttacctgatg actctccttg atgatatcaa aggagccaac 4140
gatcttgcca agttccacca gatgctgatg aagataataa tgaagtagct acagctcaac 4200
ttacctgcca accccatgcc agtcgaccta attagtacaa cctaaatcca ttataaaaaa 4260
cttaggagca aagtgattgc ctcctaagtt ccacaatgac agagatctac gatttcgaca 4320
agtcggcatg ggacatcaaa gggtcgatcg ctccgataca acctaccacc tacagtgatg 4380
gcaggctggt gccccaggtc agagtcatag atcctggtct aggtgatagg aaggatgaat 4440
gctttatgta catgtttctg ctgggggttg ttgaggacag cgatccccta gggcctccaa 4500
tcgggcgagc attcgggtcc ctgcccttag gtgttggtag atccacagca aaacccgagg 4560
aactcctcaa agaggccact gagcttgaca tagttgttag acgtacagca gggctcaatg 4620
aaaaactggt gttctacaac aacaccccac taaccctcct cacaccttgg agaaaggtcc 4680
taacaacagg gagtgtcttc aatgcaaacc aagtgtgcaa tgcggttaat ctaataccgc 4740
tggacacccc gcagaggttc cgtgttgttt atatgagcat cacccgtctt tcggataacg 4800
ggtattacac cgttcccaga agaatgctgg aattcagatc ggtcaatgca gtggccttca 4860
acctgctagt gacccttagg attgacaagg cgattggccc tgggaagatc atcgacaatg 4920
cagagcaact tcctgaggca acatttatgg tccacatcgg gaacttcagg agaaagaaga 4980
gtgaagtcta ctctgccgat tattgcaaaa tgaaaatcga aaagatgggc ctggtttttg 5040
cacttggtgg gatagggggc accagtcttc acattagaag cacaggcaaa atgagcaaga 5100
ctctccatgc acaactcggg ttcaagaaga ccttatgtta cccactgatg gatatcaatg 5160
aagaccttaa tcggttactc tggaggagca gatgcaagat agtaagaatc caggcagttt 5220
tgcagccatc agttcctcaa gaattccgca tttacgacga cgtgatcata aatgatgacc 5280
aaggactatt caaagttctg tagaccgcag tgcccagcaa tacccgaaaa cgacccccct 5340
cataatgaca gccagaaggc ccggacaaaa aagccccctc cagaagactc cacggaccaa 5400
gcgagaggcc agccagcagc cgacagcaag tgtggacacc aggcggccca agcacagaac 5460
agccccgaca caaggccacc accagccatc ccaatctgcg tcctcctcgt gggacccccg 5520
aggaccaacc ccgaaggtcg ctccgaacac agaccaccaa ccgcatcccc acagctcccg 5580
ggaaaggaac ccccagcaac tggaaggccc ctccccccct cccccaacgc aagaacccca 5640
caaccgaacc gcacaagcga ccgaggtgac ccaaccgcag gcatccgact ccttagacag 5700
atcctctccc cccggcatac taaacaaaac ttagggccaa ggaacacaca cactcgacag 5760
aacccagacc ccggcccgcg gcaccgcgcc cccacccccc gaaaaccaga gggagccccc 5820
aaccaaaccc gccggccccc ccggtgccca caggtaggca caccaacccc cgaccagacc 5880
cagcacccag ccaccgacaa tccaagacgg ggggcccccc ccaaaaaaag gcccccaggg 5940
gccgacagcc agcatcgcga ggaagcacac ccaccccaca cacgaccacg gcaaccgaac 6000
cagagtccag accaccctgg gccaccagct cccagactcg gccatcaccc cgcaaaaagg 6060
aaaggccaca acccgcgcac cccagccccg atccggcggg cagccactca acccgaacca 6120
gcacccaaga gcgatccctg ggggaccccc aaaccgcaaa agacatcagt atcccacagc 6180
ctctccaagt cccccggtct cctcctcttc tcgaagggac caaaagatca atccaccaca 6240
tccgacgaca ctcaattccc cacccccaaa ggagacaccg ggaatcccag aatcaagact 6300
catccagtgt ccatcatggg tctcaaggtg aacgtctctg ccatattcat ggcagtactg 6360
ttaactctcc aaacacccac cggtcaaatc cattggggca atctctctaa gataggggtg 6420
gtagggatag gaagtgcaag ctacaaagtt atgactcgtt ccagccatca atcattggtc 6480
ataaaattaa tgcccaatat aactctcctc aataactgca cgagggtaga gatcgcagaa 6540
tacaggagac tactgagaac agttttggaa ccaattagag atgcacttaa tgcaatgacc 6600
cagaatataa gaccggttca gagtgtagct tcaagtagga gacacaagag atttgcggga 6660
gttgtcctgg caggtgcggc cctaggcgtt gccacagctg ctcagataac agccggcatt 6720
gcacttcacc agtccatgct gaactctcaa gccatcgaca atctgagagc aagcctggaa 6780
actactaatc aggcaattga ggcaatcaga caagcagggc aggagatgat attggctgtt 6840
cagggtgtcc aagactacat caataatgag ctgataccgt ctatgaacca actatcttgt 6900
gatttaatcg gccagaagct agggctcaaa ttgctcagat actatacaga aatcctgtca 6960
ttatttggcc ccagcttacg ggaccccata tctgcggaga tatccatcca ggctttgagc 7020
tatgcgcttg gaggagatat caataaggta ttagaaaagc tcggatacag tggaggtgat 7080
ttactgggca tcttagagag cagaggaata aaggcccgga taactcacgt cgacacagag 7140
tcctacttca ttgtactcag tatagcctat ccgacgctgt ccgagattaa gggggtgatt 7200
gtccaccggc tagagggggt ctcgtacaat ataggctctc aagagtggta taccactgtg 7260
cccaagtatg ttgcaaccca agggtacctt atctcgaatt ttgatgagtc atcgtgtact 7320
ttcatgccag aggggactgt gtgcagccaa aatgccttgt acccgatgag tcctctgctc 7380
caagaatgcc tccgggggtc caccaagtcc tgtgctcgta cactcgtatc cgggtctttt 7440
gggaaccggt tcattttatc acaagggaac ctaatagcca attgtgcatc aatcctctgc 7500
aagtgttaca caacaggaac gatcattaat caagaccctg acaagatcct aacatacatt 7560
gctgccgatc actgcccggt ggtcgaggtg aacggtgtga ccatccaagt cgggagcagg 7620
aggtatccgg acgcggtgta cctgcacaga attgacctcg gtcctcccat atcattggag 7680
aggttggacg tagggacaaa tctggggaat gcaattgcta agttggagga tgccaaggaa 7740
ttgttggagt catcggacca gatattgagg agtatgaaag gtttatcgag cactagcata 7800
gtttacatcc tgattgcagt gtgtcttgga gggttgatag ggatccccgc tttaatatgt 7860
tgctgcaggg ggcgctgtaa caaaaaggga gaacaagttg gtatgtcaag accaggccta 7920
aagcctgatc ttacagggac atcaaaatcc tatgtaaggt cgctctgatc ctctacaact 7980
cttgaaacac agatttccca caagtctcct cttcgtcatc aagcaaccac cgcatccagc 8040
atcaagccca cctgaaattg tctccggctt ccctctggcc gaacgatatc ggtagttaat 8100
taaaacttag ggtgcaagat catccacaat gtcaccacaa cgagaccgaa taaatgcctt 8160
ctacaaagac aacccacatc ctaagggaag taggatagtt attaacagag aacatcttat 8220
gattgataga ccttatgttt tgctggctgt tctattcgtc atgtttctga gcttgatcgg 8280
gttgctagcc attgcaggca ttagactcca tcgtgcagcc atctacaccg cagagatcca 8340
taagagcctc agcaccaatc tagatgtaac taactcgatc gagcatcagg tcaaggacgt 8400
gctgacacca ctcttcaaga tcattggtga tgaagtgggc ctgaggacac ctcagagatt 8460
cactgaccta gtgaaattca tctctgacaa aattaaattc cttaatccgg atagggagta 8520
cgacttcaga gatctcactt ggtgtatcaa cccgccagag agaatcaaat tggattatga 8580
tcaatactgt gcagatgtgg ctgctgaaga actcatgaat gcattggtga actcaactct 8640
actggaggcc agggcaacca atcagttcct agctgtctca aagggaaact gctcagggcc 8700
cactacaatc agaggtcaat tctcaaacat gtcgctgtcc ctgttggact tgtatttaag 8760
tcgaggttac aatgtgtcat ctatagtcac tatgacatcc cagggaatgt acgggggaac 8820
ttacctagtg ggaaagccta atctgagcag taaagggtca gagttgtcac aactgagcat 8880
gcaccgagtg tttgaagtag gggttatcag aaatccgggt ttgggggctc cggtgttcca 8940
tatgacaaac tattttgagc aaccagtcag taatgatttc agcaactgca tggtggcttt 9000
gggggagctt aaattcgcag ccctctgtca cagggaagat tctatcacaa ttccctatca 9060
ggggtcaggg aaaggtgtca gcttccagct cgtcaagcta ggtgtctgga aatccccaac 9120
cgacatgcga tcctgggtcc ccctatcaac ggatgatcca gtgatagata ggctttatct 9180
ctcatctcac agaggtgtta tcgctgacaa tcaagcaaaa tgggctgtcc cgacaacacg 9240
gacagatgac aagttgcgaa tggagacatg cttccagcag gcgtgtaagg gtaaaaacca 9300
agcactctgc gagaatcccg agtgggcacc attgaaggat aacaggattc cttcatacgg 9360
ggtcttgtct gttaatctga gtctgacagt tgagcttaaa atcaaaattg cttcaggatt 9420
cgggccattg atcacacacg gttcagggat ggacctatac aaaaccaacc acaacaatgt 9480
gtattggctg actatcccgc caatgaagaa cctagcctta ggtgtaatca acacattgga 9540
gtggataccg agattcaagg ttagtcccaa cctcttcact gttccaatca aggaagcagg 9600
cgaggactgc catgccccaa catacctacc tgcggaggtg gatggtgatg tcaaactcag 9660
ttccaatctg gtaattctac ctggtcagga tctccaatat gttttggcaa cctacgatac 9720
ttccagggtt gaacatgctg tggtttatta tgtttacagc ccaagccgct cattttctta 9780
cttttatcct tttaggttgc ctataaaggg ggtcccaatc gaattacaag tggaatgctt 9840
cacatgggac aaaaaactct ggtgccgtca cttctgtgtg cttgcggact cagaatctgg 9900
tggacatatc actcactctg ggatggtggg catgggagtc agctgcacag tcactcggga 9960
agatggaacc aatcgcagat agggctgcca gtgaaccgat cacatgatgt cacccagaca 10020
tcaggcatac ccactagtgt gaaatagaca tcagaattaa gaaaaacgta gggtccaagt 10080
ggtttcccgt tatggactcg ctatctgtca accagatctt ataccctgaa gttcacctag 10140
atagcccgat agttaccaat aagatagtag ctatcctgga gtatgctcga gtccctcacg 10200
cttacagcct ggaggaccct acactgtgtc agaacatcaa gcaccgccta aaaaacggat 10260
tctccaacca aatgattata aacaatgtgg aagttgggaa tgtcatcaag tccaagctta 10320
ggagttatcc ggcccactct catattccat atccaaattg taatcaggat ttatttaaca 10380
tagaagacaa agagtcaaca aggaagatcc gtgagctcct aaaaaaggga aattcgctgt 10440
actccaaagt cagtgataag gttttccaat gcctgaggga cactaactca cggcttggcc 10500
taggctccga attgagggag gacatcaagg agaaaattat taacttggga gtttacatgc 10560
acagctccca atggtttgag ccctttctgt tttggtttac agtcaagact gagatgaggt 10620
cagtgattaa atcacaaacc catacttgcc ataggaggag acacacacct gtattcttca 10680
ctggtagttc agttgagctg ttaatctctc gtgaccttgt tgctataatc agtaaggaat 10740
ctcaacatgt atattacctg acgtttgaac tggttttgat gtattgtgat gtcatagagg 10800
ggaggttaat gacagagacc gctatgacca ttgatgctag gtatgcagaa cttctaggaa 10860
gagtcagata catgtggaaa ctgatagatg gtttcttccc tgcactcggg aatccaactt 10920
atcaaattgt agccatgctg gagccacttt cacttgctta cctgcaactg agggatataa 10980
cagtagaact cagaggtgct ttccttaacc actgctttac tgaaatacat gatgttcttg 11040
accaaaacgg gttttctgat gaaggtactt atcatgagtt aattgaagcc ctagattaca 11100
ttttcataac tgatgacata catctgacag gggagatttt ctcatttttc agaagtttcg 11160
gccaccccag acttgaagca gtaacggctg ctgaaaatgt caggaaatac atgaatcagc 11220
ctaaagtcat tgtgtatgag actctgatga aaggtcatgc catattttgt ggaatcataa 11280
tcaacggcta tcgtgacagg cacggaggca gttggccacc cctgaccctc cccctgcatg 11340
ctgcagacac aatccggaat gctcaagctt caggtgaagg gttaacacat gagcagtgcg 11400
ttgataactg gaaatcattt gctggagtga gatttggctg ttttatgcct cttagcctgg 11460
acagtgatct gacaatgtac ctaaaggaca aggcacttgc tgctctccaa agggaatggg 11520
attcagttta cccgaaagag ttcctgcgtt acgatcctcc caagggaacc gggtcacgga 11580
ggcttgtaga tgttttcctt aatgattcga gctttgaccc atatgatatg ataatgtatg 11640
tcgtaagtgg agcctacctc catgaccctg agttcaacct gtcttacagc ctgaaagaaa 11700
aggagatcaa ggaaacaggt agacttttcg ctaaaatgac ttacaaaatg agggcatgcc 11760
aagtgatcgc tgaaaatcta atctcaaacg ggattggcaa gtattttaag gacaatggga 11820
tggccaagga tgagcacgat ttgactaagg cactccacac tctggctgtc tcaggagtcc 11880
ccaaagatct caaagaaagt cacagggggg ggccagtctt aaaaacctac tcccgaagcc 11940
cagtccacac aagtaccagg aacgttaaag cagaaaaagg gtttgtagga ttccctcatg 12000
taattcggca gaatcaagac actgatcatc cggagaatat agaaacctac gagacagtca 12060
gcgcatttat cacgactgat ctcaagaagt actgccttaa ttggagatat gagaccatca 12120
gcttatttgc acagaggcta aatgagattt acggattacc ctcatttttt cagtggctgc 12180
ataagaggct tgaaacctct gtcctctatg taagtgaccc tcattgcccc cccgaccttg 12240
acgcccatgt cccgttatgc aaagtcccca atgaccaaat cttcatcaag taccctatgg 12300
gaggtataga agggtattgt cagaagctgt ggaccatcag caccattccc tacttatacc 12360
tggctgctta tgagagcggg gtaaggattg cttcgttagt gcaaggggac aatcagacca 12420
tagccgtaac aaaaagggta cccagcacat ggccttacaa ccttaagaaa cgggaagctg 12480
ctagagtaac tagagattac tttgtaattc ttaggcaaag gctacatgac attggccatc 12540
acctcaaggc aaatgagaca attgtttcat cacatttttt tgtctattca aaaggaatat 12600
attatgatgg gctacttgtg tcccaatcac tcaagagcat cgcaagatgt gtattctggt 12660
cagagactat agttgatgaa acaagggcgg catgcagtaa tattgctaca acaatggcca 12720
aaagcattga gagaggttat gaccgttatc ttgcatattc cctgaacgtc ctaaaagtga 12780
tacagcaaat tttgatctcc cttggcttca caatcaattc aaccatgacc cgggatgtag 12840
tcatacccct cctcacaaac aacgatctct taataaggat ggcactgttg cccgctccta 12900
ttgggggtat gaattatctg aatatgagca ggctgtttgt cagaaacatc ggtgatccag 12960
taacatcatc aattgctgat ctcaagagaa tgattctcgc atcactaatg cctgaagaga 13020
ccctccatca agtaatgaca caacaaccgg gggactcttc attcctagac tgggctagcg 13080
acccttactc agcaaatctt gtatgcgtcc agagcatcac tagactcctc aagaacataa 13140
ctgcaaggtt tgtcctgatc cacagtccaa acccaatgtt aaaagggtta ttccatgatg 13200
acagcaaaga agaggacgag agactggcgg cattcctcat ggacaggcat attatagtac 13260
ctagggcagc tcatgaaatc ctggatcata gtgtcacagg ggcaagagag tctattgcag 13320
gcatgctaga taccacaaaa ggcctgattc gagccagcat gaggaagggg gggttaacct 13380
ctcgagtgat aaccagattg tccaattatg actatgaaca atttagagca gggatggtgc 13440
tattgacagg aagaaagaga aatgtcctca ttgacaaaga gtcatgttca gtgcagctgg 13500
ctagagccct aagaagccat atgtgggcaa gactagctcg aggacggcct atttacggcc 13560
ttgaggtccc tgatgtacta gaatctatgc gaggccacct tattcggcgt catgagacat 13620
gtgtcatctg cgagtgtgga tcagtcaact acggatggtt ttttgtcccc tcgggttgcc 13680
aactggatga tattgacaag gaaacatcat ccttgagagt cccatatatt ggttctacca 13740
ctgatgagag aacagacatg aagctcgcct tcgtaagagc cccaagtaga tccttgcgat 13800
ctgccgttag aatagcaaca gtgtactcat gggcttacgg tgatgatgat agctcttgga 13860
acgaagcctg gttgttggca aggcaaaggg ccaatgtgag cctggaggag ctaagggtga 13920
tcactcccat ctcgacttcg actaatttag cgcataggtt gagggatcgt agcactcaag 13980
tgaaatactc aggtacatcc cttgtccgag tggcaaggta taccacaatc tccaacgaca 14040
atctctcatt tgtcatatca gataagaagg ttgatactaa ctttatatac caacaaggaa 14100
tgcttctagg gttgggtgtt ttagaaacat tgtttcgact cgagaaagat actggatcat 14160
ctaacacggt attacatctt cacgtcgaaa cagattgttg cgtgatcccg atgatagatc 14220
atcccaggat acccagctcc cgcaagctag agctgagggc agagctatgt accaacccat 14280
tgatatatga taatgcacct ttaattgaca gagatgcaac aaggctatac acccagagcc 14340
ataggaggca ccttgtggaa tttgttacat ggtccacacc ccaactatat cacattctag 14400
ctaagtccac agcactatct atgattgacc tggtaacaaa atttgagaag gaccatatga 14460
atgaaatttc agctctcata ggggatgacg atatcaatag tttcataact gagtttctgc 14520
ttatagagcc aagattattc accatctact tgggccagtg tgcagccatc aattgggcat 14580
ttgatgtaca ttatcataga ccatcaggga aatatcagat gggtgagctg ttgtcttcgt 14640
tcctttctag aatgagcaaa ggagtgttta aggtgcttgt caatgctcta agccacccaa 14700
agatctacaa gaaattctgg cattgtggta ttatagagcc tatccatggt ccttcacttg 14760
atgctcaaaa cttgcacaca actgtgtgca acatggttta cacatgctat atgacctacc 14820
tcgacctgtt gttgaatgaa gagttagaag agttcacatt tcttttgtgt gaaagcgatg 14880
aggatgtagt accggacaga ttcgacaaca tccaggcaaa acacttgtgt gttctggcag 14940
atttgtactg tcaaccaggg acctgcccac cgattcgagg tctaaggccg gtagagaaat 15000
gtgcagttct aaccgatcat atcaaggcag aggctaggtt atctccagca ggatcttcgt 15060
ggaacataaa tccaattatt gtagaccatt actcatgctc tctgacttat ctccgtcgag 15120
gatctatcaa acagataaga ttgagagttg atccaggatt catttttgac gccctcgctg 15180
aggtaaatgt cagtcagcca aaggtcggca gcgacgtcag ccatactctt tatgcccccg 15240
gtggatatga cattatggga tatctgaggc agatcaggaa ccggccaaac ccgcaggtgg 15300
aactgggccc cgtggataca tcctgcgcct tgattctttg tgacctgaaa cagaaagaca 15360
ccccgatagt ttacgcgagt gaagccttcc tctacatgac aggttacagc aacgcagagg 15420
tgctgggccg gaattgccgg tttctgcaaa gccctgacgg catggtgaag cccaagagca 15480
cccggaagta cgtggatagt aacacaatca atactatgcg caaggcaatc gacaggaatg 15540
ccgaggtgca ggttgaagta gtcaatttta aaaagaatgg acagcgattt gttaatttcc 15600
tgactatgat acctgttagg gacgaaacag gcgagtatcg atactctatg ggattccagt 15660
gcgaaacaga aaccggtaat tccgctgacg gcggcggagg atcgggtggt agtggtggtt 15720
caggaggagg atcgacccaa ggaggatcca tgcatactct ttatgccccc ggtggatatg 15780
acattatggg atatctggac cagatcggca accggccaaa cccgcaggtg gaactgggcc 15840
ccgtggatac atcctgcgcc ttgattcttt gtgacctgaa acagaaagac accccgatag 15900
tttacgcgag tgaagccttc ctctacatga caggttacag caacgcagag gtgctgggcc 15960
ggaattgccg gtttctgcaa agccctgacg gcatggtgaa gcccaagagc acccggaagt 16020
acgtggatag taacacaatc aatactatgc gcaaggcaat cgacaggaat gccgaggtgc 16080
aggttgaagt agtcaatttt aaaaagaatg gacagcgatt tgttaatttc ctgactatga 16140
tacctgttag ggacgaaaca ggcgagtatc gatactctat gggattccag tgcgaaacag 16200
aatatccgta cgacgtccca gattacgcgg gcatctcaaa tatgagcatc aaggatttca 16260
gacctccaca cgatgatgtt gcaaaattgc tcaaagatat caacacaagc aagcacaatc 16320
ttcccatttc agggggtagt ctcgccaatt atgaaatcca tgctttccgc agaatcgggt 16380
taaactcatc tgcttgctac aaagctgttg agatatcaac attaattagg agatgccttg 16440
agccagggga agacggcttg ttcttgggtg aggggtcggg ttctatgttg atcacttata 16500
aggagatact aaaactaaac aagtgcttct ataatagtgg ggtttccgcc aattctagat 16560
ctggtcaaag ggaattagca ccctatccct ccgaagttgg ccttgtcgaa cacagaatgg 16620
gagtaggtaa tattgtcaag gtgctcttta acgggaggcc cgaagtcacg tgggtaggca 16680
gtatagattg cttcaatttc atagtcagta atatccctac ctctagtgtg gggtttatcc 16740
attcagatat agagacctta cctaacaaag atactataga gaagctagag gaattggcag 16800
ccatcttatc gatggctcta ctccttggca aaataggatc aatactggtg attaagctta 16860
tgcctttcag cggggatttt gttcagggat ttataagcta tgtagggtct cattatagag 16920
aagtgaacct tgtctaccct aggtacagca acttcatatc tactgaatct tatttagtta 16980
tgacagatct caaagctaac cggctaatga atcctgaaaa gatcaagcag cagataattg 17040
aatcatctgt acggacttca cctggactta taggtcacat cctatccatt aagcaactaa 17100
gctgcataca agcaattgtg ggaggcgcgg ttagtagagg tgatatcaac cctattctga 17160
aaaaacttac acctatagag caggtgctga tcagttgcgg gttggcaatt aacggaccta 17220
aactgtgcaa agaattaatc caccatgatg ttgcctcagg gcaagatgga ttgcttaact 17280
ctatactcat cctctacagg gagttggcaa gattcaaaga caaccaaaga agtcaacaag 17340
ggatgttcca cgcttacccc gtattggtaa gtagtaggca acgagaactt gtatctagga 17400
tcactcgcaa attttggggg catattcttc tttactccgg gaacagaaag ttgataaatc 17460
ggtttatcca gaatctcaag tccggttatc tagtactaga cttacaccag aatatcttcg 17520
ttaagaatct atccaagtca gagaaacaga ttattatgac ggggggttta aaacgtgagt 17580
gggtttttaa ggtaacagtc aaggagacca aagaatggta caagttagtc ggatacagcg 17640
ctctgattaa ggattaattg gttgaactcc ggaaccctaa tcctgcccta ggtagttagg 17700
cattatttgc aatatattaa agaaaacttt gaaaatacga agtttctatt cccagctttg 17760
tctggtggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac attccgaggg 17820
gaccgtcccc tcggtaatgg cgaatgggac gcggccgccg cgctcactgg ccgtcgtttt 17880
acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 17940
ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 18000
gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 18060
ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 18120
tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 18180
gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 18240
gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 18300
ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 18360
ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 18420
tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 18480
ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 18540
tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 18600
aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 18660
tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 18720
ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 18780
tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 18840
gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 18900
aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 18960
agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 19020
acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 19080
actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 19140
accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 19200
actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 19260
cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 19320
cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 19380
gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 19440
ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 19500
tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 19560
aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 19620
gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 19680
acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 19740
tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 19800
ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 19860
atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 19920
agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 19980
cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 20040
agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 20100
acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 20160
gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 20220
ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 20280
gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 20340
gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 20400
gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 20460
tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 20520
gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 20580
ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 20640
gccaagcgcg ccagtgaatt gtaatacgac tcactata 20678
<210> 84
<211> 16914
<212> DNA
<213> Artificial Sequence
<220>
<223> pHEP-pMag-direct-nMaghigh
<400> 84
acgcttaaca acaaaaccaa agaagaagca gacatcgtca gttgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt gttcaaagtc aataaccggg tggtctcttt 120
gaagcccgag attatcgtgg atcaatatga gtacaagtac cctgctatca aagatttgaa 180
aaagccttgt ataaccctag ggaaagcccc cgatttaaac aaagcttaca aatcagtttt 240
atcaggcatg aatgccgcca aacttgatcc tgatgatgta tgctcctact tggcagcagc 300
aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc 360
acgaaaagga gacaagatca ccccagactc tctagtggag ataaagcgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaagg gaccccactg tctctgaaca 480
tgcatcttta gtcggtcttc tcctgagtct gtacagattg agcaaaatat caggacaaaa 540
cactggtaac tataagacaa acattgcgga tagaatagag cagattttcg agacagcccc 600
ttttgttaag atcgtggaac accacaccct gatgacaact cacaagatgt gtgctaattg 660
gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat 720
cgagcatctg tattcggcaa tcagagtggg cacagttgtc accgcttatg aagactgctc 780
aggactggta tcgtttacag ggttcataaa gcagatcatt ctcaccgcaa gagaagcaat 840
actatatttc ttccacaaga actttgaaga agagataagg agaatgtttg agccagggca 900
agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
gtctccttat tcatcaaatg ccgtcggtca tgtgttcaat ctcattcact ttgttggatg 1020
ctatatgggt caagtcagat ctctaaatgc cacggttatt gctgcatgtg cccctcatga 1080
gatgtctgtt ctagggggct atttgggaga ggagttcttc ggaaaaggga catttgaaag 1140
aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac tgacaaagac 1200
cgacgtggca ctggcagatg acggaactgt caactccgat gacgaggact acttctccgg 1260
tgaaaccaga agtcctgaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa 1320
gagatcacat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgaa tttttaaaca aaacgtattc gagtgactca taaggagttg aatgacaggg 1440
tgccagaaat ccatagattg tgtatatcca tcatgaaaaa aactaacact cctcctttcg 1500
aaccatccca agtatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc 1560
cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca 1620
ggctcatctc cagggagaac ccatagaagt ggacaatctc cctgaggaca tgaggcaatt 1680
tcacctggac gatgaaaaat tgtccaacct tggtgagatg gttagggtgg gcgaaggcaa 1740
gtatcgagag gactttcaga tgaatgaggg agaggacccc aacctcctgt tccaatcgta 1800
cctggacaat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa 1860
gatatggtca cagaccgtag aggaaattat atcctatgtc tcggtcaact ttcctaaccc 1920
tccaggaagg tcttcggagg ataaatcaac ccaaactact ggccgggagc tcaagaagga 1980
gacaacatcc attctttctc aaagagaaag ccaaccttca aaagccggaa tggtggctca 2040
agttgcctct ggccctccat cccttgaatg gtctgccacc aatggagagg atgatctatc 2100
agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc 2160
ttctcgatct tcaggaatat tcttgtataa ttttgagcaa ctggagatga accttgatga 2220
catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa 2280
aatcccccta agatgtgtac tgggatgggt cgctttggcc aactccaaga aattccaatt 2340
gctagtcgag gccgacaagc taagcaaaat catgcaagat gacttggatc gctacacatc 2400
atgctaaccg agtcttcgaa ttcattccct ctagataatg aaaactgaga tgtcatggag 2460
tcgatatgaa aaaaacaggc aacaccacta ataaaatgaa ctttctatgt aagatagtga 2520
aaaactgtag ggatgaggac acccaaaagc cctctcccgc gtcagcccct ccggatggcg 2580
atgacctgtg gcttccacct ccagaatatg tcccgctgaa agaactcaca agcaagaaga 2640
acatgaggaa cttttgtatc aacggggagg ttaaagtgtg cagtccgaac ggttactcat 2700
tcaggatcct gcggcatatt ctgagatcat tcgacgagat atactctggg aatcatagga 2760
tgattgggtt agtcaaagtt gttgttggac tagctttatc aggagctcca gctcctgagg 2820
gcatgaactg ggtatacaaa ttgaggagaa cccttatttt ccagtgggct gattccaggg 2880
gccctcttga aggggaggag ttggaacact ctcaagagat cacttgggac gatgatactg 2940
aattcgtcgg attgcaaatg agagtgagcg caagacaatg tcatattcaa ggcaggatct 3000
ggtgtatcga catgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac 3060
aaaggtctga agaggacaaa gactcttctc tgcttttaga ataatcagat aatatcccgc 3120
aagttcatca cttgtttact tctggaggag agaacatacg ggcttaactc caatccttgg 3180
gagcaataga acaaaaaaac acgttatgat gccattaaac cgctgcattt tatcaaagtc 3240
aagttaatta ccgttacatt ttgagcctct tggatgtgaa aaaaactatt aacatccctc 3300
aaaagactta aggaaagatg gttcctcagg ttcttttgtt tgcacccctc ctggtttctc 3360
cattgtgttt cgggaagttc cccatttaca cgataccaga caaacttggt ccctggagcc 3420
ctattgactt acaccatctc agctgtccaa ataacctggt tgtggaggac gaaggatgta 3480
ccaacctgtc cgggttctct tacatggaac ttaaagtggg atacatctca gccataaaag 3540
tgaacgggtt cacttgcaca ggtgttgtga cagaggcaga aacctacacc aactttgttg 3600
gttatgtcac aaccacattc aagagaaagc atttccgccc caccccagac gcatgtagag 3660
ccgcgtataa ctggaagatg gccggtgacc ccagatatga agagtctcta cacaatccgt 3720
accccgacta ccattggctt cgaactgtaa aaaccaccaa agagtctctc gttatcatat 3780
ccccaagtgt gacagatttg gacccatatg acaaatccct tcactcaagg gtcttccctg 3840
gcggaaattg ctcaggaata acggtgtcct cgacctactg ctcaactaat catgattaca 3900
ccatctggat gcctgagaat ctgagactag ggacatcttg tgacattttt acccatagca 3960
gagggaagag agcatccaaa ggagacaaga cttgcggctt tgtggatgaa agaggcctgt 4020
ataagtcttt aaagggagca tgcaaactca agttatgtgg agttctcgga cttagactta 4080
tggatggaac atgggtcgcg atgcaaacat cagatgagac caaatggtgc cctccaggtc 4140
agttggtgaa tttgcacgac tttcgctcag acgagattga gcatctcgtt gaggaagagt 4200
tagtcaagaa aagagaggag tgtctggatg cactagagtc catcatgacc accaagtcag 4260
tgagtttcag acgtctcagt cacctgagaa aacttgtccc tgggtttgga aaagcatata 4320
ccatattcaa caaaaccttg atggaggctg atgctcacta caagtctgtc cagacctgga 4380
atgagatcat cccctcaaaa gggtgtttga gagttgggga gaggtgtcat ccccatgtga 4440
acggggtgtt tttcaatggt ataatattag ggtctgacgg ccatgttcta atcccagaga 4500
tgcagtcatc cctcctccag caacatatgg agttgttgga atcttcagtt atccccctga 4560
tgcacccctt ggcagaccct tctacagttt tcaaagacgg tgatgaggtt gaggattttg 4620
ttgaagttca cctccccgat gtgcataaac aggtctcagg agttgacctg ggtctcccga 4680
aatgggggaa gtatgtattg atgattgcag gggccttgat tgccctgatg ttgataattt 4740
tcctgatgac atgttgcaga agagtcaatc gaccagaatc tacacaaagc aatcttggag 4800
ggacagggag aaatgtgtca gtcccttccc aaagcggaaa agtcatatct tcatgggagt 4860
catataagag tggaggcgag accagactgt gaaggccggt catccttttg acacctcaag 4920
tccagaggat aacctcctcc cggggaaaaa aactaacacc tctcgtacga ccatggtgag 4980
caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 5040
aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 5100
gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 5160
caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 5220
cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 5280
cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 5340
catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 5400
gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 5460
ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 5520
ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 5580
cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 5640
gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aactgcagtt 5700
tgctagcagg accggtacat aactataaag ggctgggtca tctaagcatt tcagtcgaga 5760
aaaaaactgt ataccaaaag aacaactaac aacacttctc atctcgagac ccatatcaag 5820
atgctggatc cgggagaggt ttatgatgac cctattgatc caattgagtc agaggctgaa 5880
cccagaggaa cccccactgt ccccaacatc ttgaggaact ctgactacaa tctcaactct 5940
cctttgattg aggactctgc caaactaatg ttagaatggt tgaaaacagg gaacagacct 6000
tatcggatga ctttgacaga caattgctcc aggtcttaca aagatttgaa agattatttc 6060
aagaaagtag atttgggttc tctcaaagtg ggaggaactg ctgcacaatc aatgatttct 6120
ctctggttgt atggagccca ctctgagtca aacaggagcc ggagatgtat aaccgacttg 6180
gcccatttct attccaagtc atcccccata gagaagctgt tgaattgtac gttaggaaac 6240
agaggcctga gaatcccacc agagggggtg ttaagttgcc ttgagagggt cgattatgat 6300
aaggcatttg ggaggtatct ggccaacacg cattcctctt atctgttttt ccatgtaatt 6360
accttataca tgaatgccct agactgggaa gaggaaaaaa ccatcctagc attatggaaa 6420
gatctaacct cagtggacac cgggaaggac ttggtcaaat tcaaagatca aatatgggga 6480
ctgctgattg tgacaaagga ctttgtttac tctcagagtt ctaactgtct ttttgacaga 6540
aactatacac tgatgctaaa ggatcttttc ttgtctcgat tcaactcctt gatgattttg 6600
ctttctcccc ctgagccccg atactcagat gacttaatat ctcagctgtg ccagctatac 6660
atcgctgggg atcaagtctt gtccttgtgt gggaactccg gctatgaagt catcaaaata 6720
ttggagccat atgtcgtgaa cagtttggtc cagagggcag agaagtttag gcctctcatc 6780
cactccttgg gagactttcc tatgtttata aaagacaagg tgaatcaact tgaagggact 6840
ttcggtccca gtgcaaaaag gttttttagg gttctagatc aattcgacaa catacatgat 6900
ctagtatttg tgtatggctg ttacagacat tgggggcacc cctatataga ttatcggaag 6960
ggtctgttga aactatatga tcaagttcac attaagaaag taatagataa gtcctaccag 7020
gagtgtttag caagtgactt ggctagaagg atcctcagat ggggatttga caagtactcc 7080
aagtggtatc tagattcgag attccttgcc cgagaccacc ccttggctcc ttatatcaag 7140
acccaaacat ggccacccaa acatatagta gacttggtgg gggacacatg gcacaagctc 7200
ccgatcacgc agatctttga gattcctgaa tcaatggacc cgtcagagat actggatgat 7260
aaatcacatt ctttcaccag aacaagacta gcttcttggc tgtcagagaa ccgagggggg 7320
cctgttccta gcgagaaggt cattatcacg gccctgtctc agccacctgt caatccccga 7380
gagtttttga aatctatcga cctcggagga ttgccagatg atgacttgat aattggcctc 7440
agaccaaagg aacgggagtt gaagattgag ggccgattct tcgctctaat gtcatggaat 7500
ctaagattat attttgtcat caccgagaag ctcttggcca actacatttt gccacttttt 7560
gacgcactga ctatgacaga caacctgaac aaggtattta aaaagttgat cgacagggtc 7620
accgggcaag ggcttttgga ctattcgagg gtcacttacg catttcacct ggactatgag 7680
aaatggaaca atcatcaaag attggagtca acggaggatg tattttctgt cctagatcag 7740
gtgtttggat tgaagagggt gttttctaga acacacgagt tttttcagaa gtcctggatc 7800
tattattcag acagatcaga cctcattggg ttacgggagg atcagatata ttgcttggat 7860
atgtccaatg gtccaacctg ctggaatggc caagacggcg ggctagaggg cttacggcag 7920
aagggctgga gtctagtcag cttattgatg atagatagag aatctcaaac caggaacaca 7980
agaaccaaga tactagctca aggagacaac caggttctgt gtccgacata catgttgtca 8040
ccgggattgt ctcaagaggg gcttctctat gagttagaga gcatatcgag gaatgcactc 8100
tcaatatacc gagctatcga ggaaggagca tctaaactgg ggctgatcat caagaaagaa 8160
gagaccatgt gtagttatga ctttctcata tatgggaaga cccccttatt tcgaggcaac 8220
atattagtac ctgaatccaa aagatgggcc cgagtctctt gcatctctaa cgaccaaata 8280
gtcaacctcg ccaatataat gtctacagta tccaccaatg cgctgacagt ggcacaacac 8340
tctcaatctc tgatcaaacc tatgagggat tttctgctca tgtcagtaca ggcagttttc 8400
cactacctgc tgtttagccc aatcttaaaa ggcagggttt ataagattct gagtgctgaa 8460
ggggagagct ttctcctagc catgtcgcgg ataatctatc tagatccttc tttgggaggg 8520
gtgtctggaa tgtctctcgg gaggttccat atacgtcagt tctcagaccc tgtctctgaa 8580
gggttgtcat tctggagaga gatctggtta agctcccacg aatcctggat tcacgcgttg 8640
tgtcaagagg ccgggaaccc cgatcttgga gagagaacac tagagagctt cactcgcctt 8700
ttagaagatc ctactacctt aaatatcaaa ggaggggcca gtcctaccat tctactcaag 8760
gatgctatca gaaaggctct gtacgacgag gtggacaagg tggaaaattc agagtttcga 8820
gaggcaatcc tgttgtccaa gacccataga gataacttta tactcttttt aaaatctgtt 8880
gagcctctgt tccctcgatt tctcagtgaa ctcttcagtt cgtccttctt gggaatacca 8940
gagtcaatta ttggactgat acaaaactcc aggacaataa gaaggcagtt tagaaagagt 9000
ctctcaagaa ctttagaaga gtccttctac aactcagaga tccacgggat taatcggatg 9060
acccagacac ctcaaagggt cgggagagtg tggccctgct cttcagagag ggcagatcta 9120
cttagggaga tctcttgggg aaggaaagtg gtgggcacga cagttcctca cccttccgag 9180
atgttggggc tgcttccaaa atcctctatt tcctgcactt gtggagcaac agggggaggc 9240
aatcctagag tttctgtatc agtactcccg tccttcgatc agtcattttt ttcacgaggt 9300
cctctaaagg gatacttggg ctcgtccacc tccatgtcaa cccagctatt ccatgcatgg 9360
gaaaaagtca ctaatgttca tgtggtgaaa agggctatat cgttaaaaga atctataaac 9420
tggttcatca atagaaattc caatttggct caaactctaa ttagaaacat catgtctctg 9480
acaggccctg atttctctct agaagaggct cctgttttca aacggacagg gtcagccttg 9540
cataggttca agtctgccag atacagcgaa ggagggtatt cttctgtttg tcctaacctt 9600
ctctctcata tctctgtcag tacagacact atgtctgatt tgacccaaga cgggaagaac 9660
tatgatttca tgtttcagcc attgatgctt tatgcgcaaa catggacatc agagctggta 9720
cagagagaca caagactgag agactccacg tttcactggc accttcgatg caacagatgt 9780
gtaaggccca ttgaggatat aacactggaa acttctcaga tcttcgagtt cccggatgtg 9840
tcaaaaagga tatccaggat ggtttctgga gccgtccctc actttcagaa gcttcctgat 9900
atccgtctaa gaccaggtga ttttgaatct ctaagtggta gagaaaagtc tcgccacata 9960
gggtcagctc aggggctctt atactcaatc ttagtagcaa ttcacgattc aggatacaat 10020
gatgggacca tcttccctgt caacatatac ggcaaggttt cccctagaga ctatttgaga 10080
gggctcgcaa gagggatctt gataggatcc tcgatttgct tcttgacacg aatgacaaat 10140
attaatatta aaagacctct tgaattgatc tcaggggtaa tttcctatat tctcctgagg 10200
ctggataatc atccctctct gtatataatg cttagagaac cgtctcttag aggagagata 10260
ttctctatcc ctcagaagat ccccgccgct tacccaacca ctatgaaaga aggcaacaga 10320
tcgatcttgt gttacctcca acacgtgcta cgctatgagc gagaagtaat cacggcgtcc 10380
ccggagaatg actggctgtg gatcttctca gacttcagaa gtgcgaaaat gacgtacttg 10440
accctcatta cctaccagtc tcacctccta ctccagaggg ttgagcgaaa cttgtctaag 10500
agtatgagag ctactctgca acaaatgggt tccttaatga ggcaagtgct gggtgggcac 10560
ggagaagaca ccttggagtc agacgacgac attcaacgat tactaaaaga ctctttgcga 10620
aggacgaggt gggtagatca agaggtgcgc catgcagcta gaaccatgag tggagattac 10680
agccccaaca agcttaagag ccatactctt tatgcccccg gtggatatga cattatggga 10740
tatctgaggc agatcaggaa ccggccaaac ccgcaggtgg aactgggccc cgtggataca 10800
tcctgcgcct tgattctttg tgacctgaaa cagaaagaca ccccgatagt ttacgcgagt 10860
gaagccttcc tctacatgac aggttacagc aacgcagagg tgctgggccg gaattgccgg 10920
tttctgcaaa gccctgacgg catggtgaag cccaagagca cccggaagta cgtggatagt 10980
aacacaatca atactatgcg caaggcaatc gacaggaatg ccgaggtgca ggttgaagta 11040
gtcaatttta aaaagaatgg acagcgattt gttaatttcc tgactatgat acctgttagg 11100
gacgaaacag gcgagtatcg atactctatg ggattccagt gcgaaacaga aatgcacact 11160
ctgtatgctc caggtggcta tgacatcatg ggctatctgg atcagatagg gaatcgtcct 11220
aatccccaag tggaacttgg accagtagac actagctgtg cactgattct ctgtgacctc 11280
aagcagaagg acacacccat cgtgtacgcc tctgaagcgt ttctgtacat gacaggctac 11340
agcaatgccg aagtcttggg gagaaattgc cgatttctgc agagtcctga cggtatggtc 11400
aaacccaagt caacgaggaa atacgtcgat tccaacacca tcaacaccat tcggaaagcc 11460
atcgatcgga atgctgaggt gcaagtggag gttgtgaact tcaagaagaa cggacagagg 11520
ttcgtgaact tcctgaccat aattccggtt agagatgaga caggagagta ccgctattcc 11580
atggggttcc agtgcgaaac agaatatccg tacgacgtcc cagattacgc gggccttaag 11640
gtatctcgca aggcaggatg ttcagaatgg gtctgctctg ctcaacaggt tgccgtctcc 11700
acctcagcca acccggcccc tgtctcagag cttgacatta gggccctatc taagaggttt 11760
caaaacccct tgatctcggg cctaagagtg gttcagtggg caaccggcgc ccattataag 11820
ctaaagccta ttctagatga tctaaatgtt ttcccatctc tctgtcttgt tgttggagac 11880
gggtcagggg gaatatcaag ggcagttctc aacatgtttc cagattctaa gcttgtgttc 11940
aacagcctat tggaggtgaa tgatctgatg gcttccggaa cacgtccact gcctccttca 12000
gcaatcatga gtggaggaga tgatatcatc tccagagtga tagactttga ctcaatctgg 12060
gaaaaaccat ccgacctgag gaacttggcc acctggagat acttccagtc agttcaaaag 12120
caggtcaaca tgtcgtatga cctcattatt tgtgatgcag aagttactga tattgcatct 12180
atcaaccgga taactctgtt gatgtctgat ttcgcattgt ctatagatgg accactttat 12240
ctggtcttca aaacttacgg gactatgcta gtgaacccag actataaagc tatccaacat 12300
ctgtcaagag cgttcccttc ggtcacaggg tttataaccc aagtaacttc gtccttttct 12360
tctgagctgt acctccggtt ctccaaacga gggaagtttt ttagggatgc tgagtacttg 12420
acctcttcca cccttcggga gatgagcctt gtgttattca attgtagcag ccccaaaagt 12480
gagatgcaga gagctcgttc cttaaactat caagatcttg taaggggatt tcctgaagag 12540
atcatatcaa atccttacaa tgaaatgatc ataactctga ttgacagtga tgtagagtcc 12600
ttcctggtcc acaagatggt ggatgatctt gagttacaga ggggaactct gtctaaagtg 12660
gctatcatta tatccatcat gatcgttttt tccaacagag tcttcaacat ttccaaacct 12720
ttgactgacc ccttgttcta tcccccatct gatcctaaaa tcctgaggca cttcaacata 12780
tgttgcagta ctatgatgta tctatctacc gctttaggcg acgtccctag cttcgcaaga 12840
cttcatgacc tatataatag acctataact tattacttca gaaagcaagt tattcgaggg 12900
aatatctatc tatcttggag ctggtccgat gacaccccag tgttcaagag ggtagcctgt 12960
aattctagct tgagtctgtc atctcactgg atcaggttga tttacaagat agtgaagact 13020
accagactcg ttggccgcat agaagatcta tcaggagagg tagaacgaca cctgcatggg 13080
tacaacagat ggatcaccct cgaggatatc cgatctagat catccctact agactacagt 13140
tgtttgtaag ccggacatta atgaaagcct gtacatgcta aaattcttgt atgatgcatc 13200
ttgaaaaaaa caagatcttg aatcagaacc tctggttgtt tgattgtttt ttttatcttt 13260
attgtttatt tgttaagcgt gggtcggcat ggcatctcca cctcctcgcg gtccgacctg 13320
ggcatccgaa ggaggacgca cgtccactcg gatggctaag ggagggcggg taccacgcgc 13380
tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 13440
cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 13500
cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca 13560
ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 13620
gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 13680
caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 13740
cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 13800
tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 13860
acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 13920
gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 13980
ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 14040
tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 14100
ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 14160
ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 14220
aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 14280
gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 14340
ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 14400
gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 14460
cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 14520
cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 14580
acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 14640
caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 14700
taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 14760
ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 14820
aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 14880
agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 14940
atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 15000
tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 15060
tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 15120
gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 15180
taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 15240
aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 15300
ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 15360
catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 15420
ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 15480
ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 15540
agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 15600
taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 15660
atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 15720
cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 15780
ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 15840
accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 15900
gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 15960
gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 16020
agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 16080
tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 16140
gctatgacca tgattacgcc aagcgcgctg tacgggccag atatacgcgt tgacattgat 16200
tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 16260
agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 16320
gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 16380
gacgtcaatg ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 16440
atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 16500
cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 16560
ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 16620
cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 16680
atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 16740
ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg 16800
cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagtgtt 16860
aagcgtctga tgagtccgtg aggacgaaac tataggaaag gaattcctat agtc 16914
<210> 85
<211> 16992
<212> DNA
<213> Artificial Sequence
<220>
<223> pHEP-pMag-linker-nMag
<400> 85
acgcttaaca acaaaaccaa agaagaagca gacatcgtca gttgcaaagc aaaaatgtaa 60
cacccctaca atggatgccg acaagattgt gttcaaagtc aataaccggg tggtctcttt 120
gaagcccgag attatcgtgg atcaatatga gtacaagtac cctgctatca aagatttgaa 180
aaagccttgt ataaccctag ggaaagcccc cgatttaaac aaagcttaca aatcagtttt 240
atcaggcatg aatgccgcca aacttgatcc tgatgatgta tgctcctact tggcagcagc 300
aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc 360
acgaaaagga gacaagatca ccccagactc tctagtggag ataaagcgta ctgatgtaga 420
agggaattgg gctctgacag gaggcatgga actgacaagg gaccccactg tctctgaaca 480
tgcatcttta gtcggtcttc tcctgagtct gtacagattg agcaaaatat caggacaaaa 540
cactggtaac tataagacaa acattgcgga tagaatagag cagattttcg agacagcccc 600
ttttgttaag atcgtggaac accacaccct gatgacaact cacaagatgt gtgctaattg 660
gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat 720
cgagcatctg tattcggcaa tcagagtggg cacagttgtc accgcttatg aagactgctc 780
aggactggta tcgtttacag ggttcataaa gcagatcatt ctcaccgcaa gagaagcaat 840
actatatttc ttccacaaga actttgaaga agagataagg agaatgtttg agccagggca 900
agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 960
gtctccttat tcatcaaatg ccgtcggtca tgtgttcaat ctcattcact ttgttggatg 1020
ctatatgggt caagtcagat ctctaaatgc cacggttatt gctgcatgtg cccctcatga 1080
gatgtctgtt ctagggggct atttgggaga ggagttcttc ggaaaaggga catttgaaag 1140
aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac tgacaaagac 1200
cgacgtggca ctggcagatg acggaactgt caactccgat gacgaggact acttctccgg 1260
tgaaaccaga agtcctgaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa 1320
gagatcacat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1380
attcgccgaa tttttaaaca aaacgtattc gagtgactca taaggagttg aatgacaggg 1440
tgccagaaat ccatagattg tgtatatcca tcatgaaaaa aactaacact cctcctttcg 1500
aaccatccca agtatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc 1560
cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca 1620
ggctcatctc cagggagaac ccatagaagt ggacaatctc cctgaggaca tgaggcaatt 1680
tcacctggac gatgaaaaat tgtccaacct tggtgagatg gttagggtgg gcgaaggcaa 1740
gtatcgagag gactttcaga tgaatgaggg agaggacccc aacctcctgt tccaatcgta 1800
cctggacaat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa 1860
gatatggtca cagaccgtag aggaaattat atcctatgtc tcggtcaact ttcctaaccc 1920
tccaggaagg tcttcggagg ataaatcaac ccaaactact ggccgggagc tcaagaagga 1980
gacaacatcc attctttctc aaagagaaag ccaaccttca aaagccggaa tggtggctca 2040
agttgcctct ggccctccat cccttgaatg gtctgccacc aatggagagg atgatctatc 2100
agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc 2160
ttctcgatct tcaggaatat tcttgtataa ttttgagcaa ctggagatga accttgatga 2220
catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa 2280
aatcccccta agatgtgtac tgggatgggt cgctttggcc aactccaaga aattccaatt 2340
gctagtcgag gccgacaagc taagcaaaat catgcaagat gacttggatc gctacacatc 2400
atgctaaccg agtcttcgaa ttcattccct ctagataatg aaaactgaga tgtcatggag 2460
tcgatatgaa aaaaacaggc aacaccacta ataaaatgaa ctttctatgt aagatagtga 2520
aaaactgtag ggatgaggac acccaaaagc cctctcccgc gtcagcccct ccggatggcg 2580
atgacctgtg gcttccacct ccagaatatg tcccgctgaa agaactcaca agcaagaaga 2640
acatgaggaa cttttgtatc aacggggagg ttaaagtgtg cagtccgaac ggttactcat 2700
tcaggatcct gcggcatatt ctgagatcat tcgacgagat atactctggg aatcatagga 2760
tgattgggtt agtcaaagtt gttgttggac tagctttatc aggagctcca gctcctgagg 2820
gcatgaactg ggtatacaaa ttgaggagaa cccttatttt ccagtgggct gattccaggg 2880
gccctcttga aggggaggag ttggaacact ctcaagagat cacttgggac gatgatactg 2940
aattcgtcgg attgcaaatg agagtgagcg caagacaatg tcatattcaa ggcaggatct 3000
ggtgtatcga catgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac 3060
aaaggtctga agaggacaaa gactcttctc tgcttttaga ataatcagat aatatcccgc 3120
aagttcatca cttgtttact tctggaggag agaacatacg ggcttaactc caatccttgg 3180
gagcaataga acaaaaaaac acgttatgat gccattaaac cgctgcattt tatcaaagtc 3240
aagttaatta ccgttacatt ttgagcctct tggatgtgaa aaaaactatt aacatccctc 3300
aaaagactta aggaaagatg gttcctcagg ttcttttgtt tgcacccctc ctggtttctc 3360
cattgtgttt cgggaagttc cccatttaca cgataccaga caaacttggt ccctggagcc 3420
ctattgactt acaccatctc agctgtccaa ataacctggt tgtggaggac gaaggatgta 3480
ccaacctgtc cgggttctct tacatggaac ttaaagtggg atacatctca gccataaaag 3540
tgaacgggtt cacttgcaca ggtgttgtga cagaggcaga aacctacacc aactttgttg 3600
gttatgtcac aaccacattc aagagaaagc atttccgccc caccccagac gcatgtagag 3660
ccgcgtataa ctggaagatg gccggtgacc ccagatatga agagtctcta cacaatccgt 3720
accccgacta ccattggctt cgaactgtaa aaaccaccaa agagtctctc gttatcatat 3780
ccccaagtgt gacagatttg gacccatatg acaaatccct tcactcaagg gtcttccctg 3840
gcggaaattg ctcaggaata acggtgtcct cgacctactg ctcaactaat catgattaca 3900
ccatctggat gcctgagaat ctgagactag ggacatcttg tgacattttt acccatagca 3960
gagggaagag agcatccaaa ggagacaaga cttgcggctt tgtggatgaa agaggcctgt 4020
ataagtcttt aaagggagca tgcaaactca agttatgtgg agttctcgga cttagactta 4080
tggatggaac atgggtcgcg atgcaaacat cagatgagac caaatggtgc cctccaggtc 4140
agttggtgaa tttgcacgac tttcgctcag acgagattga gcatctcgtt gaggaagagt 4200
tagtcaagaa aagagaggag tgtctggatg cactagagtc catcatgacc accaagtcag 4260
tgagtttcag acgtctcagt cacctgagaa aacttgtccc tgggtttgga aaagcatata 4320
ccatattcaa caaaaccttg atggaggctg atgctcacta caagtctgtc cagacctgga 4380
atgagatcat cccctcaaaa gggtgtttga gagttgggga gaggtgtcat ccccatgtga 4440
acggggtgtt tttcaatggt ataatattag ggtctgacgg ccatgttcta atcccagaga 4500
tgcagtcatc cctcctccag caacatatgg agttgttgga atcttcagtt atccccctga 4560
tgcacccctt ggcagaccct tctacagttt tcaaagacgg tgatgaggtt gaggattttg 4620
ttgaagttca cctccccgat gtgcataaac aggtctcagg agttgacctg ggtctcccga 4680
aatgggggaa gtatgtattg atgattgcag gggccttgat tgccctgatg ttgataattt 4740
tcctgatgac atgttgcaga agagtcaatc gaccagaatc tacacaaagc aatcttggag 4800
ggacagggag aaatgtgtca gtcccttccc aaagcggaaa agtcatatct tcatgggagt 4860
catataagag tggaggcgag accagactgt gaaggccggt catccttttg acacctcaag 4920
tccagaggat aacctcctcc cggggaaaaa aactaacacc tctcgtacga ccatggtgag 4980
caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 5040
aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 5100
gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 5160
caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 5220
cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 5280
cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 5340
catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 5400
gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 5460
ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 5520
ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 5580
cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 5640
gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aactgcagtt 5700
tgctagcagg accggtacat aactataaag ggctgggtca tctaagcatt tcagtcgaga 5760
aaaaaactgt ataccaaaag aacaactaac aacacttctc atctcgagac ccatatcaag 5820
atgctggatc cgggagaggt ttatgatgac cctattgatc caattgagtc agaggctgaa 5880
cccagaggaa cccccactgt ccccaacatc ttgaggaact ctgactacaa tctcaactct 5940
cctttgattg aggactctgc caaactaatg ttagaatggt tgaaaacagg gaacagacct 6000
tatcggatga ctttgacaga caattgctcc aggtcttaca aagatttgaa agattatttc 6060
aagaaagtag atttgggttc tctcaaagtg ggaggaactg ctgcacaatc aatgatttct 6120
ctctggttgt atggagccca ctctgagtca aacaggagcc ggagatgtat aaccgacttg 6180
gcccatttct attccaagtc atcccccata gagaagctgt tgaattgtac gttaggaaac 6240
agaggcctga gaatcccacc agagggggtg ttaagttgcc ttgagagggt cgattatgat 6300
aaggcatttg ggaggtatct ggccaacacg cattcctctt atctgttttt ccatgtaatt 6360
accttataca tgaatgccct agactgggaa gaggaaaaaa ccatcctagc attatggaaa 6420
gatctaacct cagtggacac cgggaaggac ttggtcaaat tcaaagatca aatatgggga 6480
ctgctgattg tgacaaagga ctttgtttac tctcagagtt ctaactgtct ttttgacaga 6540
aactatacac tgatgctaaa ggatcttttc ttgtctcgat tcaactcctt gatgattttg 6600
ctttctcccc ctgagccccg atactcagat gacttaatat ctcagctgtg ccagctatac 6660
atcgctgggg atcaagtctt gtccttgtgt gggaactccg gctatgaagt catcaaaata 6720
ttggagccat atgtcgtgaa cagtttggtc cagagggcag agaagtttag gcctctcatc 6780
cactccttgg gagactttcc tatgtttata aaagacaagg tgaatcaact tgaagggact 6840
ttcggtccca gtgcaaaaag gttttttagg gttctagatc aattcgacaa catacatgat 6900
ctagtatttg tgtatggctg ttacagacat tgggggcacc cctatataga ttatcggaag 6960
ggtctgttga aactatatga tcaagttcac attaagaaag taatagataa gtcctaccag 7020
gagtgtttag caagtgactt ggctagaagg atcctcagat ggggatttga caagtactcc 7080
aagtggtatc tagattcgag attccttgcc cgagaccacc ccttggctcc ttatatcaag 7140
acccaaacat ggccacccaa acatatagta gacttggtgg gggacacatg gcacaagctc 7200
ccgatcacgc agatctttga gattcctgaa tcaatggacc cgtcagagat actggatgat 7260
aaatcacatt ctttcaccag aacaagacta gcttcttggc tgtcagagaa ccgagggggg 7320
cctgttccta gcgagaaggt cattatcacg gccctgtctc agccacctgt caatccccga 7380
gagtttttga aatctatcga cctcggagga ttgccagatg atgacttgat aattggcctc 7440
agaccaaagg aacgggagtt gaagattgag ggccgattct tcgctctaat gtcatggaat 7500
ctaagattat attttgtcat caccgagaag ctcttggcca actacatttt gccacttttt 7560
gacgcactga ctatgacaga caacctgaac aaggtattta aaaagttgat cgacagggtc 7620
accgggcaag ggcttttgga ctattcgagg gtcacttacg catttcacct ggactatgag 7680
aaatggaaca atcatcaaag attggagtca acggaggatg tattttctgt cctagatcag 7740
gtgtttggat tgaagagggt gttttctaga acacacgagt tttttcagaa gtcctggatc 7800
tattattcag acagatcaga cctcattggg ttacgggagg atcagatata ttgcttggat 7860
atgtccaatg gtccaacctg ctggaatggc caagacggcg ggctagaggg cttacggcag 7920
aagggctgga gtctagtcag cttattgatg atagatagag aatctcaaac caggaacaca 7980
agaaccaaga tactagctca aggagacaac caggttctgt gtccgacata catgttgtca 8040
ccgggattgt ctcaagaggg gcttctctat gagttagaga gcatatcgag gaatgcactc 8100
tcaatatacc gagctatcga ggaaggagca tctaaactgg ggctgatcat caagaaagaa 8160
gagaccatgt gtagttatga ctttctcata tatgggaaga cccccttatt tcgaggcaac 8220
atattagtac ctgaatccaa aagatgggcc cgagtctctt gcatctctaa cgaccaaata 8280
gtcaacctcg ccaatataat gtctacagta tccaccaatg cgctgacagt ggcacaacac 8340
tctcaatctc tgatcaaacc tatgagggat tttctgctca tgtcagtaca ggcagttttc 8400
cactacctgc tgtttagccc aatcttaaaa ggcagggttt ataagattct gagtgctgaa 8460
ggggagagct ttctcctagc catgtcgcgg ataatctatc tagatccttc tttgggaggg 8520
gtgtctggaa tgtctctcgg gaggttccat atacgtcagt tctcagaccc tgtctctgaa 8580
gggttgtcat tctggagaga gatctggtta agctcccacg aatcctggat tcacgcgttg 8640
tgtcaagagg ccgggaaccc cgatcttgga gagagaacac tagagagctt cactcgcctt 8700
ttagaagatc ctactacctt aaatatcaaa ggaggggcca gtcctaccat tctactcaag 8760
gatgctatca gaaaggctct gtacgacgag gtggacaagg tggaaaattc agagtttcga 8820
gaggcaatcc tgttgtccaa gacccataga gataacttta tactcttttt aaaatctgtt 8880
gagcctctgt tccctcgatt tctcagtgaa ctcttcagtt cgtccttctt gggaatacca 8940
gagtcaatta ttggactgat acaaaactcc aggacaataa gaaggcagtt tagaaagagt 9000
ctctcaagaa ctttagaaga gtccttctac aactcagaga tccacgggat taatcggatg 9060
acccagacac ctcaaagggt cgggagagtg tggccctgct cttcagagag ggcagatcta 9120
cttagggaga tctcttgggg aaggaaagtg gtgggcacga cagttcctca cccttccgag 9180
atgttggggc tgcttccaaa atcctctatt tcctgcactt gtggagcaac agggggaggc 9240
aatcctagag tttctgtatc agtactcccg tccttcgatc agtcattttt ttcacgaggt 9300
cctctaaagg gatacttggg ctcgtccacc tccatgtcaa cccagctatt ccatgcatgg 9360
gaaaaagtca ctaatgttca tgtggtgaaa agggctatat cgttaaaaga atctataaac 9420
tggttcatca atagaaattc caatttggct caaactctaa ttagaaacat catgtctctg 9480
acaggccctg atttctctct agaagaggct cctgttttca aacggacagg gtcagccttg 9540
cataggttca agtctgccag atacagcgaa ggagggtatt cttctgtttg tcctaacctt 9600
ctctctcata tctctgtcag tacagacact atgtctgatt tgacccaaga cgggaagaac 9660
tatgatttca tgtttcagcc attgatgctt tatgcgcaaa catggacatc agagctggta 9720
cagagagaca caagactgag agactccacg tttcactggc accttcgatg caacagatgt 9780
gtaaggccca ttgaggatat aacactggaa acttctcaga tcttcgagtt cccggatgtg 9840
tcaaaaagga tatccaggat ggtttctgga gccgtccctc actttcagaa gcttcctgat 9900
atccgtctaa gaccaggtga ttttgaatct ctaagtggta gagaaaagtc tcgccacata 9960
gggtcagctc aggggctctt atactcaatc ttagtagcaa ttcacgattc aggatacaat 10020
gatgggacca tcttccctgt caacatatac ggcaaggttt cccctagaga ctatttgaga 10080
gggctcgcaa gagggatctt gataggatcc tcgatttgct tcttgacacg aatgacaaat 10140
attaatatta aaagacctct tgaattgatc tcaggggtaa tttcctatat tctcctgagg 10200
ctggataatc atccctctct gtatataatg cttagagaac cgtctcttag aggagagata 10260
ttctctatcc ctcagaagat ccccgccgct tacccaacca ctatgaaaga aggcaacaga 10320
tcgatcttgt gttacctcca acacgtgcta cgctatgagc gagaagtaat cacggcgtcc 10380
ccggagaatg actggctgtg gatcttctca gacttcagaa gtgcgaaaat gacgtacttg 10440
accctcatta cctaccagtc tcacctccta ctccagaggg ttgagcgaaa cttgtctaag 10500
agtatgagag ctactctgca acaaatgggt tccttaatga ggcaagtgct gggtgggcac 10560
ggagaagaca ccttggagtc agacgacgac attcaacgat tactaaaaga ctctttgcga 10620
aggacgaggt gggtagatca agaggtgcgc catgcagcta gaaccatgag tggagattac 10680
agccccaaca agcttaagag ccatactctt tatgcccccg gtggatatga cattatggga 10740
tatctgaggc agatcaggaa ccggccaaac ccgcaggtgg aactgggccc cgtggataca 10800
tcctgcgcct tgattctttg tgacctgaaa cagaaagaca ccccgatagt ttacgcgagt 10860
gaagccttcc tctacatgac aggttacagc aacgcagagg tgctgggccg gaattgccgg 10920
tttctgcaaa gccctgacgg catggtgaag cccaagagca cccggaagta cgtggatagt 10980
aacacaatca atactatgcg caaggcaatc gacaggaatg ccgaggtgca ggttgaagta 11040
gtcaatttta aaaagaatgg acagcgattt gttaatttcc tgactatgat acctgttagg 11100
gacgaaacag gcgagtatcg atactctatg ggattccagt gcgaaacaga aaccggtaat 11160
tccgctgacg gcggcggagg atcgggtggt agtggtggtt caggaggagg atcgacccaa 11220
ggaggatcca tgcatactct ttatgccccc ggtggatatg acattatggg atatctggac 11280
cagatcggca accggccaaa cccgcaggtg gaactgggcc ccgtggatac atcctgcgcc 11340
ttgattcttt gtgacctgaa acagaaagac accccgatag tttacgcgag tgaagccttc 11400
ctctacatga caggttacag caacgcagag gtgctgggcc ggaattgccg gtttctgcaa 11460
agccctgacg gcatggtgaa gcccaagagc acccggaagt acgtggatag taacacaatc 11520
aatactatgc gcaaggcaat cgacaggaat gccgaggtgc aggttgaagt agtcaatttt 11580
aaaaagaatg gacagcgatt tgttaatttc ctgactatga tacctgttag ggacgaaaca 11640
ggcgagtatc gatactctat gggattccag tgcgaaacag aatatccgta cgacgtccca 11700
gattacgcgg gccttaaggt atctcgcaag gcaggatgtt cagaatgggt ctgttctgct 11760
caacaggttg ccgtctccac ctcagccaac ccggcccctg tctcagagct tgacattagg 11820
gccctatcta agaggtttca aaaccccttg atctcgggcc taagagtggt tcagtgggca 11880
accggcgccc attataagct aaagcctatt ctagatgatc taaatgtttt cccatctctc 11940
tgtcttgttg ttggagacgg gtcaggggga atatcaaggg cagttctcaa catgtttcca 12000
gattctaagc ttgtgttcaa cagcctattg gaggtgaatg atctgatggc ttccggaaca 12060
cgtccactgc ctccttcagc aatcatgagt ggaggagatg atatcatctc cagagtgata 12120
gactttgact caatctggga aaaaccatcc gacctgagga acttggccac ctggagatac 12180
ttccagtcag ttcaaaagca ggtcaacatg tcgtatgacc tcattatttg tgatgcagaa 12240
gttactgata ttgcatctat caaccggata actctgttga tgtctgattt cgcattgtct 12300
atagatggac cactttatct ggtcttcaaa acttacggga ctatgctagt gaacccagac 12360
tataaagcta tccaacatct gtcaagagcg ttcccttcgg tcacagggtt tataacccaa 12420
gtaacttcgt ccttttcttc tgagctgtac ctccggttct ccaaacgagg gaagtttttt 12480
agggatgctg agtacttgac ctcttccacc cttcgggaga tgagccttgt gttattcaat 12540
tgtagcagcc ccaaaagtga gatgcagaga gctcgttcct taaactatca agatcttgta 12600
aggggatttc ctgaagagat catatcaaat ccttacaatg aaatgatcat aactctgatt 12660
gacagtgatg tagagtcctt cctggtccac aagatggtgg atgatcttga gttacagagg 12720
ggaactctgt ctaaagtggc tatcattata tccatcatga tcgttttttc caacagagtc 12780
ttcaacattt ccaaaccttt gactgacccc ttgttctatc ccccatctga tcctaaaatc 12840
ctgaggcact tcaacatatg ttgcagtact atgatgtatc tatctaccgc tttaggcgac 12900
gtccctagct tcgcaagact tcatgaccta tataatagac ctataactta ttacttcaga 12960
aagcaagtta ttcgagggaa tatctatcta tcttggagct ggtccgatga caccccagtg 13020
ttcaagaggg tagcctgtaa ttctagcttg agtctgtcat ctcactggat caggttgatt 13080
tacaagatag tgaagactac cagactcgtt ggccgcatag aagatctatc aggagaggta 13140
gaacgacacc tgcatgggta caacagatgg atcaccctcg aggatatccg atctagatca 13200
tccctactag actacagttg tttgtaagcc ggacattaat gaaagcctgt acatgctaaa 13260
attcttgtat gatgcatctt gaaaaaaaca agatcttgaa tcagaacctc tggttgtttg 13320
attgtttttt ttatctttat tgtttatttg ttaagcgtgg gtcggcatgg catctccacc 13380
tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga tggctaaggg 13440
agggcgggta ccacgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 13500
ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 13560
gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac 13620
gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 13680
acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 13740
ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 13800
gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 13860
tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 13920
ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 13980
gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 14040
gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc 14100
gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 14160
aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 14220
tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 14280
aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 14340
aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 14400
tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 14460
aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 14520
tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 14580
ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 14640
taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 14700
agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 14760
caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 14820
tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 14880
gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 14940
cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 15000
caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 15060
ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 15120
aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 15180
gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 15240
atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 15300
tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 15360
gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 15420
actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 15480
gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 15540
agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 15600
ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 15660
aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 15720
cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 15780
gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 15840
cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 15900
cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 15960
gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 16020
aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 16080
actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 16140
cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 16200
aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgctgta cgggccagat 16260
atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 16320
ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 16380
gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 16440
caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 16500
cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 16560
ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 16620
tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 16680
gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 16740
gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 16800
tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 16860
taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 16920
cccaagctgg ctagtgttaa gcgtctgatg agtccgtgag gacgaaacta taggaaagga 16980
attcctatag tc 16992
<210> 86
<211> 8976
<212> DNA
<213> Artificial Sequence
<220>
<223> Oct3-measles-Sox2-l-myc
<400> 86
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggc 120
gggacacctg gcttcagatt ttgccttctc gccccctcca ggtggtggag gtgatgggcc 180
aggggggccg gagccgggct gggttgatcc tcggacctgg ctaagcttcc aaggccctcc 240
tggagggcca ggaatcgggc cgggggttgg gccaggctct gaggtgtggg ggattccccc 300
atgccccccg ccgtatgagt tctgtggggg gatggcgtac tgtgggcccc aggttggagt 360
ggggctagtg ccccaaggcg gcttggagac ctctcagcct gagggcgaag caggagtcgg 420
ggtggagagc aactccgatg gggcctcccc ggagccctgc accgtcaccc ctggtgccgt 480
gaagctggag aaggagaagc tggagcaaaa cccggaggag tcccaggaca tcaaagctct 540
gcagaaagaa ctcgagcaat ttgccaagct cctgaagcag aagaggatca ccctgggata 600
tacacaggcc gatgtggggc tcaccctggg ggttctattt gggaaggtat tcagccaaac 660
gaccatctgc cgctttgagg ctctgcagct tagcttcaag aacatgtgta agctgcggcc 720
cttgctgcag aagtgggtgg aggaagctga caacaatgaa aatcttcagg agatatgcaa 780
agcagaaacc ctcgtgcagg cccgaaagag aaagcgaacc agtatcgaga accgagtgag 840
aggcaacctg gagaatttgt tcctgcagtg cccgaaaccc acactgcagc agatcagcca 900
cattgcccag cagcttgggc tcgagaagga tgtggtccga gtgtggttct gtaaccggcg 960
ccagaagggc aagcgatcaa gcagcgacta tgcacaacga gaggattttg aggctgctgg 1020
gtctcctttc tcagggggac cagtgtcctt tcctctggcc ccagggcccc attttggtac 1080
cccaggctat gggagccctc acttcactgc actgtactcc tcggtccctt tccctgaggg 1140
ggaagccttt ccccctgtct ctgtcaccac tctgggctct cccatgcatt caaactgata 1200
agacgtcgag aggccgagga ccagaacaac atccgcctac cctccatcat tgttataaaa 1260
aacttaggaa ccaggtccac acagccgcca gccaaccaac catccactcc cacgactgga 1320
gttcgaacga gatggccaca cttttgagga gcttagcatt gttcaaaaga aacaaggaca 1380
aaccacccat tacatcagga tccggtggag ccatcagagg aatcaaacac attattatag 1440
taccaattcc tggagattcc tcaattacca ctcgatccag actactggac cggttggtca 1500
ggttaattgg aaacccggat gtgagcgggc ccaaactaac aggggcacta ataggtatat 1560
tatccttatt tgtggagtct ccaggtcaat tgattcagag gatcaccgat gaccctgacg 1620
ttagcatcag gctgttagag gttgttcaga gtgaccagtc acaatctggc cttaccttcg 1680
catcaagagg taccaacatg gaggatgagg cggaccaata cttttcacat gatgatccaa 1740
gcagtagtga tcaatccagg tccggatggt tcgagaacaa ggaaatctca gatattgaag 1800
tgcaagaccc tgagggattc aacatgattc tgggtaccat tctagcccag atctgggtct 1860
tgctcgcaaa ggcggttacg gccccagaca cggcagctga ttcggagcta agaaggtgga 1920
taaagtacac ccaacaaaga agggtagttg gtgaatttag attggagaga aaatggttgg 1980
atgtggtgag gaacaggatt gccgaggacc tctctttacg ccgattcatg gtggctctaa 2040
tcctggatat caagaggaca cccgggaaca aacctaggat tgctgaaatg atatgtgaca 2100
ttgatacata tatcgtagag gcaggattag ccagttttat cctgactatt aagtttggga 2160
tagaaactat gtatcctgct cttggactgc atgaatttgc tggtgagtta tccacacttg 2220
agtccttgat gaatctttac cagcaaatgg gagaaactgc accctacatg gtaatcctag 2280
agaactcaat tcagaacaag ttcagtgcag gatcataccc tctgctctgg agctatgcca 2340
tgggagtagg agtggaactt gaaaactcca tgggaggttt gaactttggt cgatcttact 2400
ttgatccagc atattttaga ttagggcaag agatggtgag gaggtcagct ggaaaggtca 2460
gttccacatt ggcatccgaa ctcggtatca ctgccgagga tgcaaggctt gtttcagaga 2520
ttgcaatgca tactactgag gacaggatca gtagagcggt cggacccaga caagcccaag 2580
tgtcatttct acacggtgat caaagtgaga atgagctacc aggattgggg ggcaaggaag 2640
ataggagggt caaacagggt cggggagaag ccagggagag ctacagagaa accgggtcca 2700
gcagagcaag tgatgcgaga gctgcccatc ctccaaccag catgccccta gacattgaca 2760
ctgcatcgga gtcaggccaa gatccgcagg acagtcgaag gtcagctgac gccctgctca 2820
ggctgcaagc catggcagga atcttggaag aacaaggctc agacacggac acccctaggg 2880
tatacaatga cagagatctt ctagactagg tgcgagaggc cgaggaccag aacaacatcc 2940
gcctaccctc catcattgtt ataaaaaact taggaaccag gtccacacag ccgccagcca 3000
accaaccatc cactcccacg actggagccg atggcagaag agcaggcacg ccatgtcaaa 3060
aacggactgg aatgcatccg ggctctcaag gccgagccca tcggctcact ggccgtcgag 3120
gaagccatgg cagcatggtc agaaatatca gacaacccag gacaggaccg agccacctgc 3180
aaggaagagg aggcaggcag ttcgggtctc agcaaaccat gcctctcagc aattggatca 3240
actgaaggcg gtgcacctcg catccgcggt cagggatctg gagaaagcga tgacgacgct 3300
gaaactttgg gaatcccctc aagaaatctc caggcatcaa gcactgggtt acagtgttat 3360
catgtttatg atcacagcgg tgaagcggtt aagggaatcc aagatgctga ctctatcatg 3420
gttcaatcag gccttgatgg tgatagcacc ctctcaggag gagacgatga atctgaaaac 3480
agcgatgtgg atattggcga acctgatacc gagggatatg ctatcactga ccggggatct 3540
gctcccatct ctatggggtt cagggcttct gatgttgaaa ctgcagaagg aggggagatc 3600
cacgagctcc tgaaactcca atccagaggc aacaactttc cgaagcttgg gaaaactctc 3660
aatgttcctc cgcccccgaa ccccagtagg gccagcactt ccgagacacc cattaaaaag 3720
ggcacagacg cgagattggc ctcatttgga acggagatcg cgtctttatt gacaggtggt 3780
gcaacccaat gtgctcgaaa gtcaccctcg gaaccatcag ggccaggtgc acctgcgggg 3840
aatgtccccg agtgtgtgag caatgccgca ctgatacagg agtggacacc cgaatctggt 3900
accacaatct ccccgagatc ccagaataat gaagaagggg gagactatta tgatgatgag 3960
ctgttctccg atgtccaaga catcaaaaca gccttggcca aaatacacga ggataatcag 4020
aagataatct ccaagctaga atcattgctg ttattgaagg gagaagttga gtcaattaag 4080
aagcagatca acaggcaaaa tatcagcata tccaccctgg aaggacacct ctcaagcatc 4140
atgattgcca ttcctggact tgggaaggat cccaacgacc ccactgcaga tgtcgaactc 4200
aatcccgacc tgaaacccat cataggcaga gattcaggcc gagcactggc cgaagttctc 4260
aagaagcccg ttgccagccg acaactccag ggaatgacta atggacggac cagttccaga 4320
ggacagctgc tgaaggaatt tcaactaaag ccgatcggga aaaaggtgag ctcagccgtc 4380
gggtttgttc ctgacaccgg ccctgcatca cgcagtgtaa tccgctccat tataaaatcc 4440
agccggctag aggaggatcg gaagcgttac ctgatgactc tccttgatga tatcaaagga 4500
gccaacgatc ttgccaagtt ccaccagatg ctgatgaaga taataatgaa gtagctacag 4560
ctcaacttac ctgccaaccc catgccagtc gacctaatta gtacaaccta aatccattat 4620
aaaaaactta ggagcaaagt gattgcctcc taagttccac aatgacagag atctacgatt 4680
tcgacaagtc ggcatgggac atcaaagggt cgatcgctcc gatacaacct accacctaca 4740
gtgatggcag gctggtgccc caggtcagag tcatagatcc tggtctaggt gataggaagg 4800
atgaatgctt tatgtacatg tttctgctgg gggttgttga ggacagcgat tccctagggc 4860
ctccaatcgg gcgagcattc gggtccctgc ccttaggtgt tggtagatcc acagcaaaac 4920
ccgagaaact cctcaaagag gccactgagc ttgacatagt tgttagacgt acagcagggc 4980
tcaatgaaaa actggtgttc tacaacaaca ccccactaac cctcctcaca ccttggagaa 5040
aggtcctaac aacagggagt gtcttcaatg caaaccaagt gtgcaatgcg gttaatctaa 5100
taccgctgga caccccgcag aggttccgtg ttgtttatat gagcatcacc cgtctttcgg 5160
ataacgggta ttacaccgtt cccagaagaa tgctggaatt cagatcggtc aatgcagtgg 5220
ccttcaacct gctagtgacc cttaggattg acaaggcgat tggccctggg aagatcatcg 5280
acaatgcaga gcaacttcct gaggcaacat ttatggtcca catcgggaac ttcaggagaa 5340
agaagagtga agtctactct gccgattatt gcaaaatgaa aatcgaaaag atgggcctgg 5400
tttttgcact tggtgggata gggggcacca gtcttcacat tagaagcaca ggcaaaatga 5460
gcaagactct ccatgcacaa ctcgggttca agaagacctt atgttaccca ctgatggata 5520
tcaatgaaga ccttaatcgg ttactctgga ggagcagatg caagatagta agaatccagg 5580
cagttttgca gccatcagtt cctcaagaat tccgcattta cgacgacgtg atcataaatg 5640
atgaccaagg actattcaaa gttctgtaga ccgcagtgcc cagcaatacc cgaaaacgac 5700
ccccctcata atgacagcca gaaggcccgg acaaaaaagc cccctccaga agactccacg 5760
gaccaagcga gaggccagcc agcagccgac agcaagtgtg gacaccaggc ggcccaagca 5820
cagaacagcc ccgacacaag gccaccacca gccatcccaa tctgcgtcct cctcgtggga 5880
cccccgagga ccaaccccga aggtcgctcc gaacacagac caccaaccgc atccccacag 5940
ctcccgggaa aggaaccccc agcaactgga aggcccctcc ccccctcccc caacgcaaga 6000
accccacaac cgaaccgcac aagcgaccga ggtgacccaa ccgcaggcat ccgactcctt 6060
agacagatcc tctccccccg gcatactaaa caaaacttag ggccaaggaa cacacacact 6120
cgacagaacc cagaccccgg cccgcggcac cgcgccccca ccccccgaaa accagaggga 6180
gcccccaacc aaacccgccg gcccccccgg tgcccacagg taggcacacc aacccccgac 6240
cagacccagc acccagccac cgacaatcca agacgggggg ccccccccaa aaaaaggccc 6300
ccaggggccg acagccagca tcgatgtaca acatgatgga gacggagctg aagccgccgg 6360
gcccgcagca aacttcgggg ggcggcggcg gcaactccac cgcggcggcg gccggcggca 6420
accagaaaaa cagcccggac cgcgtcaagc ggcccatgaa tgccttcatg gtgtggtccc 6480
gcgggcagcg gcgcaagatg gcccaggaga accccaagat gcacaactcg gagatcagca 6540
agcgcctggg cgccgagtgg aaacttttgt cggagacgga gaagcggccg ttcatcgacg 6600
aggctaagcg gctgcgagcg ctgcacatga aggagcaccc ggattataaa taccggcccc 6660
ggcggaaaac caagacgctc atgaagaagg ataagtacac gctgcccggc gggctgctgg 6720
cccccggcgg caatagcatg gcgagcgggg tcggggtggg cgccggcctg ggcgcgggcg 6780
tgaaccagcg catggacagt tacgcgcaca tgaacggctg gagcaacggc agctacagca 6840
tgatgcagga ccagctgggc tacccgcagc acccgggcct caatgcgcac ggcgcagcgc 6900
agatgcagcc catgcaccgc tacgacgtga gcgccctgca gtacaactcc atgaccagct 6960
cgcagaccta catgaacggc tcgcccacct acagcatgtc ctactcgcag cagggcaccc 7020
ctggcatggc tcttggctcc atgggttcgg tggtcaagtc cgaggccagc tccagccccc 7080
ctgtggttac ctcttcctcc cactccaggg cgccctgcca ggccggggac ctccgggaca 7140
tgatcagcat gtatctcccc ggcgccgagg tgccggaacc cgccgccccc agcagacttc 7200
acatgtccca gcactaccag agcggcccgg tgcccggcac ggccattaac ggcacactgc 7260
ccctctcaca catgtgataa cggtcctccc atatcattgg agaggttgga cgtagggaca 7320
aatctgggga atgcaattgc taagttggag gatgccaagg aattgttgga gtcatcggac 7380
cagatattga ggagtatgaa aggtttatcg agcactagca tagtttacat cctgattgca 7440
gtgtgtcttg gagggttgat agggatcccc gctttaatat gttgctgcag ggggcgctgt 7500
aacaaaaagg gagaacaagt tggtatgtca agaccaggcc taaagcctga tcttacaggg 7560
acatcaaaat cctatgtaag gtcgctctga tcctctacaa ctcttgaaac acagatttcc 7620
cacaagtctc ctcttcgtca tcaagcaacc accgcatcca gcatcaagcc cacctgaaat 7680
tgtctccggc ttccctctgg ccgaacgata tcggtagtta attaaaactt agggtgcaag 7740
atcatccaca atggactacg actcgtacca gcactatttc tacgactatg actgcgggga 7800
ggatttctac cgctccacgg cgcccagcga ggacatctgg aagaaattcg agctggtgcc 7860
atcgcccccc acgtcgccgc cctggggctt gggtcccggc gcaggggacc cggcccccgg 7920
gattggtccc ccggagccgt ggcccggagg gtgcaccgga gacgaagcgg aatcccgggg 7980
ccactcgaaa ggctggggca ggaactacgc ctccatcata cgccgtgact gcatgtggag 8040
cggcttctcg gcccgggaac ggctggagag agctgtgagc gaccggctcg ctcctggcgc 8100
gccccggggg aacccgccca aggcgtccgc cgccccggac tgcactccca gcctcgaagc 8160
cggcaacccg gcgcccgccg ccccctgtcc gctgggcgaa cccaagaccc aggcctgctc 8220
cgggtccgag agcccaagcg actcggagaa tgaagaaatt gatgttgtga cagtagagaa 8280
gaggcagtct ctgggtattc ggaagccggt caccatcacg gtgcgagcag accccctgga 8340
tccctgcatg aagcatttcc acatctccat ccatcagcaa cagcacaact atgctgcccg 8400
ttttcctcca gaaagctgct cccaagaaga ggcttcagag aggggtcccc aagaagaggt 8460
tctggagaga gatgctgcag gggaaaagga agatgaggag gatgaagaga ttgtgagtcc 8520
cccacctgta gaaagtgagg ctgcccagtc ctgccacccc aaacctgtca gttctgatac 8580
tgaggatgtg accaagagga agaatcacaa cttcctggag cgcaagaggc ggaatgacct 8640
gcgttcgcga ttcttggcgc tgagggacca ggtgcccacc ctggccagct gctccaaggc 8700
ccccaaagta gtgatcctaa gcaaggcctt ggaatacttg caagccctgg tgggggctga 8760
gaagaggatg gctacagaga aaagacagct ccgatgccgg cagcagcagt tgcagaaaag 8820
aattgcatac ctcactggct actaatagcg ctctgattaa ggattaattg gttgaactcc 8880
ggaaccctaa tcctgcccta ggtagttagg cattatttgc aatatattaa agaaaacttt 8940
gaaaatacga agtttctatt cccagctttg tctggt 8976
<210> 87
<211> 12102
<212> DNA
<213> Artificial Sequence
<220>
<223> egfp-klf4-measles-L-pMag-linker-nMaghigh
<400> 87
accaaacaaa gttgggtaag gatagatcaa tcaatgatca tattctagta cacttaggat 60
tcaagatcct attatcaggg acaagagcag gattagggat atccgagggc gcgccatggt 120
gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 180
cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 240
gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 300
gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 360
cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 420
ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 480
ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 540
ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 600
caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 660
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 720
gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 780
ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagacgt 840
cgagaggccg aggaccagaa caacatccgc ctaccctcca tcattgttat aaaaaactta 900
ggaaccaggt ccacacagcc gccagccaac caaccatcca ctcccacgac tggagttcga 960
agcagatggc tgtcagcgac gcgctgctcc catctttctc cacgttcgcg tctggcccgg 1020
cgggaaggga gaagacactg cgtcaagcag gtgccccgaa taaccgctgg cgggaggagc 1080
tctcccacat gaagcgactt cccccagtgc ttcccggccg cccctatgac ctggcggcgg 1140
cgaccgtggc cacagacctg gagagcggcg gagccggtgc ggcttgcggc ggtagcaacc 1200
tggcgcccct acctcggaga gagaccgagg agttcaacga tctcctggac ctggacttta 1260
ttctctccaa ttcgctgacc catcctccgg agtcagtggc cgccaccgtg tcctcgtcag 1320
cgtcagcctc ctcttcgtcg tcgccgtcga gcagcggccc tgccagcgcg ccctccacct 1380
gcagcttcac ctatccgatc cgggccggga acgacccggg cgtggcgccg ggcggcacgg 1440
gcggaggcct cctctatggc agggagtccg ctccccctcc gacggctccc ttcaacctgg 1500
cggacatcaa cgacgtgagc ccctcgggcg gcttcgtggc cgagctcctg cggccagaat 1560
tggacccggt gtacattccg ccgcagcagc cgcagccgcc aggtggcggg ctgatgggca 1620
agttcgtgct gaaggcgtcg ctgagcgccc ctggcagcga gtacggcagc ccgtcggtca 1680
tcagcgtcag caaaggcagc cctgacggca gccacccggt ggtggtggcg ccctacaacg 1740
gcgggccgcc gcgcacgtgc cccaagatca agcaggaggc ggtctcttcg tgcacccact 1800
tgggcgctgg accccctctc agcaatggcc accggccggc tgcacacgac ttccccctgg 1860
ggcggcagct ccccagcagg actaccccga ccctgggtct tgaggaagtg ctgagcagca 1920
gggactgtca ccctgccctg ccgcttcctc ccggcttcca tccccacccg gggcccaatt 1980
acccatcctt cctgcccgat cagatgcagc cgcaagtccc gccgctccat taccaagagc 2040
tcatgccacc cggttcctgc atgccagagg agcccaagcc aaagagggga agacgatcgt 2100
ggccccggaa aaggaccgcc acccacactt gtgattacgc gggctgcggc aaaacctaca 2160
caaagagttc ccatctcaag gcacacctgc gaacccacac aggtgagaaa ccttaccact 2220
gtgactggga cggctgtgga tggaaattcg cccgctcaga tgaactgacc aggcactacc 2280
gtaaacacac ggggcaccgc ccgttccagt gccaaaaatg cgaccgagca ttttccaggt 2340
cggaccacct cgccttacac atgaagaggc atttttaata ggacgtcgag aggccgagga 2400
ccagaacaac atccgcctac gatatcggta gttaattaaa acttagggtg caagatcatc 2460
cacaatgtca ccacaacgag accgaataaa tgccttctac aaagacaacc cacatcctaa 2520
gggaagtagg atagttatta acagagaaca tcttatgatt gatagacctt atgttttgct 2580
ggctgttcta ttcgtcatgt ttctgagctt gatcgggttg ctagccattg caggcattag 2640
actccatcgt gcagccatct acaccgcaga gatccataag agcctcagca ccaatctaga 2700
tgtaactaac tcgatcgagc atcaggtcaa ggacgtgctg acaccactct tcaagatcat 2760
tggtgatgaa gtgggcctga ggacacctca gagattcact gacctagtga aattcatctc 2820
tgacaaaatt aaattcctta atccggatag ggagtacgac ttcagagatc tcacttggtg 2880
tatcaacccg ccagagagaa tcaaattgga ttatgatcaa tactgtgcag atgtggctgc 2940
tgaagaactc atgaatgcat tggtgaactc aactctactg gaggccaggg caaccaatca 3000
gttcctagct gtctcaaagg gaaactgctc agggcccact acaatcagag gtcaattctc 3060
aaacatgtcg ctgtccctgt tggacttgta tttaagtcga ggttacaatg tgtcatctat 3120
agtcactatg acatcccagg gaatgtacgg gggaacttac ctagtgggaa agcctaatct 3180
gagcagtaaa gggtcagagt tgtcacaact gagcatgcac cgagtgtttg aagtaggggt 3240
tatcagaaat ccgggtttgg gggctccggt gttccatatg acaaactatt ttgagcaacc 3300
agtcagtaat gatttcagca actgcatggt ggctttgggg gagcttaaat tcgcagccct 3360
ctgtcacagg gaagattcta tcacaattcc ctatcagggg tcagggaaag gtgtcagctt 3420
ccagctcgtc aagctaggtg tctggaaatc cccaaccgac atgcgatcct gggtccccct 3480
atcaacggat gatccagtga tagataggct ttatctctca tctcacagag gtgttatcgc 3540
tgacaatcaa gcaaaatggg ctgtcccgac aacacggaca gatgacaagt tgcgaatgga 3600
gacatgcttc cagcaggcgt gtaagggtaa aatacaagca ctctgcgaga atcccgagtg 3660
ggcaccattg aaggataaca ggattccttc atacggggtc ttgtctgttg acctgagtct 3720
gacagttgag cttaaaatca aaattgcttc aggattcggg ccattgatca cacacggttc 3780
agggatggac ctatacaaaa ccaaccacaa caatgtgtat tggctgacta tcccgccaat 3840
gaagaaccta gccttaggtg taatcaacac attggagtgg ataccgagat tcaaggttag 3900
tccctatctc ttcactgttc caatcaagga agcaggcggc gactgccatg ccccaacata 3960
cctacctgcg gaggtggatg gtgatgtcaa actcagttcc aatctggtaa ttctacctgg 4020
tcaggatctc caatatgttt tggcaaccta cgatacttcc agggttgaac atgctgtggt 4080
ttattatgtt tacagcccaa gccgctcatt ttcttacttt tatcctttta ggttgcctat 4140
aaagggggtc ccaatcgaat tacaagtgga atgcttcaca tgggacaaaa aactctggtg 4200
ccgtcacttc tgtgtgcttg cggactcaga atctggtgga catatcactc actctgggat 4260
ggtgggcatg ggagtcagct gcacagtcac tcgggaagat ggaaccaatc gcagataggg 4320
ctgccagtga accgatcaca tgatgtcacc cagacatcag gcatacccac tagtgtgaaa 4380
tagacatcag aattaagaaa aacgtagggt ccaagtggtt tcccgttatg gactcgctat 4440
ctgtcaacca gatcttatac cctgaagttc acctagatag cccgatagtt accaataaga 4500
tagtagctat cctggagtat gctcgagtcc ctcacgctta cagcctggag gaccctacac 4560
tgtgtcagaa catcaagcac cgcctaaaaa acggattctc caaccaaatg attataaaca 4620
atgtggaagt tgggaatgtc atcaagtcca agcttaggag ttatccggcc cactctcata 4680
ttccatatcc aaattgtaat caggatttat ttaacataga agacaaagag tcaacaagga 4740
agatccgtga gctcctaaaa aagggaaatt cgctgtactc caaagtcagt gataaggttt 4800
tccaatgcct gagggacact aactcacggc ttggcctagg ctccgaattg agggaggaca 4860
tcaaggagaa aattattaac ttgggagttt acatgcacag ctcccaatgg tttgagccct 4920
ttctgttttg gtttacagtc aagactgaga tgaggtcagt gattaaatca caaacccata 4980
cttgccatag gaggagacac acacctgtat tcttcactgg tagttcagtt gagctgttaa 5040
tctctcgtga ccttgttgct ataatcagta aggaatctca acatgtatat tacctgacgt 5100
ttgaactggt tttgatgtat tgtgatgtca tagaggggag gttaatgaca gagaccgcta 5160
tgaccattga tgctaggtat gcagaacttc taggaagagt cagatacatg tggaaactga 5220
tagatggttt cttccctgca ctcgggaatc caacttatca aattgtagcc atgctggagc 5280
cactttcact tgcttacctg caactgaggg atataacagt agaactcaga ggtgctttcc 5340
ttaaccactg ctttactgaa atacatgatg ttcttgacca aaacgggttt tctgatgaag 5400
gtacttatca tgagttaatt gaagccctag attacatttt cataactgat gacatacatc 5460
tgacagggga gattttctca tttttcagaa gtttcggcca ccccagactt gaagcagtaa 5520
cggctgctga aaatgtcagg aaatacatga atcagcctaa agtcattgtg tatgagactc 5580
tgatgaaagg tcatgccata ttttgtggaa tcataatcaa cggctatcgt gacaggcacg 5640
gaggcagttg gccacccctg accctccccc tgcatgctgc agacacaatc cggaatgctc 5700
aagcttcagg tgaagggtta acacatgagc agtgcgttga taactggaaa tcatttgctg 5760
gagtgagatt tggctgtttt atgcctctta gcctggacag tgatctgaca atgtacctaa 5820
aggacaaggc acttgctgct ctccaaaggg aatgggattc agtttacccg aaagagttcc 5880
tgcgttacga tcctcccaag ggaaccgggt cacggaggct tgtagatgtt ttccttaatg 5940
attcgagctt tgacccatat gatatgataa tgtatgtcgt aagtggagcc tacctccatg 6000
accctgagtt caacctgtct tacagcctga aagaaaagga gatcaaggaa acaggtagac 6060
ttttcgctaa aatgacttac aaaatgaggg catgccaagt gatcgctgaa aatctaatct 6120
caaacgggat tggcaagtat tttaaggaca atgggatggc caaggatgag cacgatttga 6180
ctaaggcact ccacactctg gctgtctcag gagtccccaa agatctcaaa gaaagtcaca 6240
ggggggggcc agtcttaaaa acctactccc gaagcccagt ccacacaagt accaggaacg 6300
ttaaagcaga aaaagggttt gtaggattcc ctcatgtaat tcggcagaat caagacactg 6360
atcatccgga gaatatagaa acctacgaga cagtcagcgc atttatcacg actgatctca 6420
agaagtactg ccttaattgg agatatgaga ccatcagctt atttgcacag aggctaaatg 6480
agatttacgg attaccctca ttttttcagt ggctgcataa gaggcttgaa acctctgtcc 6540
tctatgtaag tgaccctcat tgcccccccg accttgacgc ccatgtcccg ttatgcaaag 6600
tccccaatga ccaaatcttc atcaagtacc ctatgggagg tatagaaggg tattgtcaga 6660
agctgtggac catcagcacc attccctact tatacctggc tgcttatgag agcggggtaa 6720
ggattgcttc gttagtgcaa ggggacaatc agaccatagc cgtaacaaaa agggtaccca 6780
gcacatggcc ttacaacctt aagaaacggg aagctgctag agtaactaga gattactttg 6840
taattcttag gcaaaggcta catgacattg gccatcacct caaggcaaat gagacaattg 6900
tttcatcaca tttttttgtc tattcaaaag gaatatatta tgatgggcta cttgtgtccc 6960
aatcactcaa gagcatcgca agatgtgtat tctggtcaga gactatagtt gatgaaacaa 7020
gggcggcatg cagtaatatt gctacaacaa tggccaaaag cattgagaga ggttatgacc 7080
gttatcttgc atattccctg aacgtcctaa aagtgataca gcaaattttg atctcccttg 7140
gcttcacaat caattcaacc atgacccggg atgtagtcat acccctcctc acaaacaacg 7200
atctcttaat aaggatggca ctgttgcccg ctcctattgg gggtatgaat tatctgaata 7260
tgagcaggct gtttgtcaga aacatcggtg atccagtaac atcatcaatt gctgatctca 7320
agagaatgat tctcgcatca ctaatgcctg aagagaccct ccatcaagta atgacacaac 7380
aaccggggga ctcttcattc ctagactggg ctagcgaccc ttactcagca aatcttgtat 7440
gcgtccagag catcactaga ctcctcaaga acataactgc aaggtttgtc ctgatccaca 7500
gtccaaaccc aatgttaaaa gggttattcc atgatgacag caaagaagag gacgagagac 7560
tggcggcatt cctcatggac aggcatatta tagtacctag ggcagctcat gaaatcctgg 7620
atcatagtgt cacaggggca agagagtcta ttgcaggcat gctagatacc acaaaaggcc 7680
tgattcgagc cagcatgagg aagggggggt taacctctcg agtgataacc agattgtcca 7740
attatgacta tgaacaattt agagcaggga tggtgctatt gacaggaaga aagagaaatg 7800
tcctcattga caaagagtca tgttcagtgc agctggctag agccctaaga agccatatgt 7860
gggcaagact agctcgagga cggcctattt acggccttga ggtccctgat gtactagaat 7920
ctatgcgagg ccaccttatt cggcgtcatg agacatgtgt catctgcgag tgtggatcag 7980
tcaactacgg atggtttttt gtcccctcgg gttgccaact ggatgatatt gacaaggaaa 8040
catcatcctt gagagtccca tatattggtt ctaccactga tgagagaaca gacatgaagc 8100
tcgccttcgt aagagcccca agtagatcct tgcgatctgc cgttagaata gcaacagtgt 8160
actcatgggc ttacggtgat gatgatagct cttggaacga agcctggttg ttggcaaggc 8220
aaagggccaa tgtgagcctg gaggagctaa gggtgatcac tcccatctcg acttcgacta 8280
atttagcgca taggttgagg gatcgtagca ctcaagtgaa atactcaggt acatcccttg 8340
tccgagtggc aaggtatacc acaatctcca acgacaatct ctcatttgtc atatcagata 8400
agaaggttga tactaacttt atataccaac aaggaatgct tctagggttg ggtgttttag 8460
aaacattgtt tcgactcgag aaagatactg gatcatctaa cacggtatta catcttcacg 8520
tcgaaacaga ttgttgcgtg atcccgatga tagatcatcc caggataccc agctcccgca 8580
agctagagct gagggcagag ctatgtacca acccattgat atatgataat gcacctttaa 8640
ttgacagaga tgcaacaagg ctatacaccc agagccatag gaggcacctt gtggaatttg 8700
ttacatggtc cacaccccaa ctatatcaca ttctagctaa gtccacagca ctatctatga 8760
ttgacctggt aacaaaattt gagaaggacc atatgaatga aatttcagct ctcatagggg 8820
atgacgatat caatagtttc ataactgagt ttctgcttat agagccaaga ttattcacca 8880
tctacttggg ccagtgtgca gccatcaatt gggcatttga tgtacattat catagaccat 8940
cagggaaata tcagatgggt gagctgttgt cttcgttcct ttctagaatg agcaaaggag 9000
tgtttaaggt gcttgtcaat gctctaagcc acccaaagat ctacaagaaa ttctggcatt 9060
gtggtattat agagcctatc catggtcctt cacttgatgc tcaaaacttg cacacaactg 9120
tgtgcaacat ggtttacaca tgctatatga cctacctcga cctgttgttg aatgaagagt 9180
tagaagagtt cacatttctt ttgtgtgaaa gcgatgagga tgtagtaccg gacagattcg 9240
acaacatcca ggcaaaacac ttgtgtgttc tggcagattt gtactgtcaa ccagggacct 9300
gcccaccgat tcgaggtcta aggccggtag agaaatgtgc agttctaacc gatcatatca 9360
aggcagaggc taggttatct ccagcaggat cttcgtggaa cataaatcca attattgtag 9420
accattactc atgctctctg acttatctcc gtcgaggatc tatcaaacag ataagattga 9480
gagttgatcc aggattcatt tttgacgccc tcgctgaggt aaatgtcagt cagccaaagg 9540
tcggcagcga cgtcagccat actctttatg cccccggtgg atatgacatt atgggatatc 9600
tgaggcagat caggaaccgg ccaaacccgc aggtggaact gggccccgtg gatacatcct 9660
gcgccttgat tctttgtgac ctgaaacaga aagacacccc gatagtttac gcgagtgaag 9720
ccttcctcta catgacaggt tacagcaacg cagaggtgct gggccggaat tgccggtttc 9780
tgcaaagccc tgacggcatg gtgaagccca agagcacccg gaagtacgtg gatagtaaca 9840
caatcaatac tatgcgcaag gcaatcgaca ggaatgccga ggtgcaggtt gaagtagtca 9900
attttaaaaa gaatggacag cgatttgtta atttcctgac tatgatacct gttagggacg 9960
aaacaggcga gtatcgatac tctatgggat tccagtgcga aacagaaacc ggtaattccg 10020
ctgacggcgg cggaggatcg ggtggtagtg gtggttcagg aggaggatcg acccaaggag 10080
gatccatgca tactctttat gcccccggtg gatatgacat tatgggatat ctggaccaga 10140
tcggcaaccg gccaaacccg caggtggaac tgggccccgt ggatacatcc tgcgccttga 10200
ttctttgtga cctgaaacag aaagacaccc cgatagttta cgcgagtgaa gccttcctct 10260
acatgacagg ttacagcaac gcagaggtgc tgggccggaa ttgccggttt ctgcaaagcc 10320
ctgacggcat ggtgaagccc aagagcaccc ggaagtacgt ggatagtaac acaatcaata 10380
ctatccgcaa ggcaatcgac aggaatgccg aggtgcaggt tgaagtagtc aattttaaaa 10440
agaatggaca gcgatttgtt aatttcctga ctatcatacc tgttagggac gaaacaggcg 10500
agtatcgata ctctatggga ttccagtgcg aaacagaata tccgtacgac gtcccagatt 10560
acgcgggcat ctcaaatatg agcatcaagg atttcagacc tccacacgat gatgttgcaa 10620
aattgctcaa agatatcaac acaagcaagc acaatcttcc catttcaggg ggtagtctcg 10680
ccaattatga aatccatgct ttccgcagaa tcgggttaaa ctcatctgct tgctacaaag 10740
ctgttgagat atcaacatta attaggagat gccttgagcc aggggaagac ggcttgttct 10800
tgggtgaggg gtcgggttct atgttgatca cttataagga gatactaaaa ctaaacaagt 10860
gcttctataa tagtggggtt tccgccaatt ctagatctgg tcaaagggaa ttagcaccct 10920
atccctccga agttggcctt gtcgaacaca gaatgggagt aggtaatatt gtcaaggtgc 10980
tctttaacgg gaggcccgaa gtcacgtggg taggcagtat agattgcttc aatttcatag 11040
tcagtaatat ccctacctct agtgtggggt ttatccattc agatatagag accttaccta 11100
acaaagatac tatagagaag ctagaggaat tggcagccat cttatcgatg gctctactcc 11160
ttggcaaaat aggatcaata ctggtgatta agcttatgcc tttcagcggg gattttgttc 11220
agggatttat aagctatgta gggtctcatt atagagaagt gaaccttgtc taccctaggt 11280
acagcaactt catatctact gaatcttatt tagttatgac agatctcaaa gctaaccggc 11340
taatgaatcc tgaaaagatc aagcagcaga taattgaatc atctgtacgg acttcacctg 11400
gacttatagg tcacatccta tccattaagc aactaagctg catacaagca attgtgggag 11460
gcgcggttag tagaggtgat atcaacccta ttctgaaaaa acttacacct atagagcagg 11520
tgctgatcag ttgcgggttg gcaattaacg gacctaaact gtgcaaagaa ttaatccacc 11580
atgatgttgc ctcagggcaa gatggattgc ttaactctat actcatcctc tacagggagt 11640
tggcaagatt caaagacaac caaagaagtc aacaagggat gttccacgct taccccgtat 11700
tggtaagtag taggcaacga gaacttgtat ctaggatcac tcgcaaattt tgggggcata 11760
ttcttcttta ctccgggaac agaaagttga taaatcggtt tatccagaat ctcaagtccg 11820
gttatctagt actagactta caccagaata tcttcgttaa gaatctatcc aagtcagaga 11880
aacagattat tatgacgggg ggtttaaaac gtgagtgggt ttttaaggta acagtcaagg 11940
agaccaaaga atggtacaag ttagtcggat acagcgctct gattaaggat taattggttg 12000
aactccggaa ccctaatcct gccctaggta gttaggcatt atttgcaata tattaaagaa 12060
aactttgaaa atacgaagtt tctattccca gctttgtctg gt 12102

Claims (20)

1.一种病毒蛋白质基因，该病毒蛋白质基因在具有酶活性的病毒蛋白质的可导入外源基因的区域以可表达的方式插入有编码光开关蛋白质的基因，其中，所述光开关蛋白质包含至少2个亚基，编码各亚基的基因介以接头连接，或者不介以接头而直接连接。
2.如权利要求1所述的病毒蛋白质基因，其中，所述接头为10～100个氨基酸残基。
3.如权利要求1或2所述的病毒蛋白质基因，其中，所述编码光开关蛋白质的基因为Magnet基因。
4.如权利要求3所述的病毒蛋白质基因，其中，该基因可通过照射波长450～490nm的光来调节所述病毒蛋白质的活性。
5.如权利要求1～4中的任一项所述的病毒蛋白质基因，其中，具有酶活性的病毒蛋白质为具有聚合酶或蛋白质酶结构域的病毒蛋白质。
6.如权利要求1～5中的任一项所述的病毒蛋白质基因，其中，所述病毒为RNA病毒。
7.如权利要求1～6中的任一项所述的病毒蛋白质基因，其中，所述病毒蛋白质为RNA依赖性RNA聚合酶。
8.如权利要求7所述的病毒蛋白质基因，其中，所述RNA依赖性RNA聚合酶为RNA依赖性RNA聚合酶L蛋白质。
9.如权利要求7或8所述的病毒蛋白质基因，其中，所述可导入外源基因的区域为Ln结构域与Lc结构域之间的区域。
10.如权利要求1～9中的任一项所述的病毒蛋白质基因，其中，在所述编码光开关蛋白质的基因的上游和/或下游还包含接头。
11.一种核酸，其中，该核酸编码权利要求1～10中的任一项所述的病毒蛋白质基因。
12.一种载体，其中，包含编码权利要求1～10中的任一项所述的病毒蛋白质基因的核酸。
13.一种病毒或病毒载体，其中，包含权利要求1～10中的任一项所述的病毒蛋白质基因。
14.一种试剂盒，其中，包含权利要求11所述的核酸、权利要求11所述的载体、或权利要求12所述的病毒或病毒载体。
15.一种细胞，其中，包含权利要求11所述的核酸。
16.如权利要求15所述的细胞，其中，所述细胞为iPS细胞或其后代细胞。
17.一种通过病毒载体暂时性感染细胞的方法，其中，包括：
使包含导入基因的权利要求13所述的病毒或病毒载体感染细胞的工序；
在光照下培养已感染的细胞的工序；以及
在无光照条件下培养已感染的细胞的工序。
18.一种使导入基因表达的方法，其中，包括：
使包含导入基因的权利要求13所述的病毒或病毒载体感染细胞的工序；以及
在光照下培养已感染的细胞的工序。
19.一种iPS的制造方法，其中，包括：
使包含选自Oct3/4、Sox2、Klf4、l-Myc(或c-Myc)、Nanog和Lin28的至少1种导入基因的权利要求13所述的病毒或病毒载体感染体细胞的工序；以及
在光照下培养已感染的细胞的工序。
20.如权利要求18或19所述的方法，其中，还包括：在无光照条件下培养已感染的细胞的工序。
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111733170A (zh) * 2020-07-01 2020-10-02 青岛农业大学 一种表达荧光素酶的重组犬麻疹病毒
CN112852817A (zh) * 2021-03-04 2021-05-28 中国人民解放军陆军特色医学中心 一种用于III-A型CRISPR-Csm系统中RNA编辑的组合物及应用
CN114058607A (zh) * 2020-07-31 2022-02-18 上海科技大学 一种用于c到u碱基编辑的融合蛋白及其制备方法和应用
WO2022037666A1 (zh) * 2020-08-21 2022-02-24 北京大学 Rdr蛋白在肿瘤治疗中的应用
EP4263825A1 (en) * 2021-02-19 2023-10-25 Beam Therapeutics, Inc. Recombinant rabies viruses for gene therapy

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121079418A (zh) * 2023-05-10 2025-12-05 国立大学法人东京大学 光开关蛋白质

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017053629A2 (en) * 2015-09-22 2017-03-30 William Marsh Rice University Light-controlled gene delivery with virus vectors through incorporation of optogenetic proteins and genetic insertion of non-conformationally constrained peptides
US20180163195A1 (en) * 2016-12-12 2018-06-14 Trustees Of Boston University Inducible dimerization of recombinases

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017053629A2 (en) * 2015-09-22 2017-03-30 William Marsh Rice University Light-controlled gene delivery with virus vectors through incorporation of optogenetic proteins and genetic insertion of non-conformationally constrained peptides
US20180163195A1 (en) * 2016-12-12 2018-06-14 Trustees Of Boston University Inducible dimerization of recombinases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DUPREX W.P.等: "Modulating the Function of the Measles Virus RNA-Dependent RNA Polymerase by Insertion of Green Fluorescent Protein into the Open Reading Frame", 《JOURNAL OF VIROLOGY》 *
HIRAMOTO T.等: "Newly developed measles virus vector can simultaneously transfer multiple genes into human hematopoietic cells and induce ground state like pluripotent stem cells", 《MOLECULAR THERAPY》 *
ZHOU X.X.等: "Optical control of protein activity by fluorescent protein domains", 《SCIENCE》 *

Cited By (7)

* Cited by examiner, † Cited by third party
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CN111733170A (zh) * 2020-07-01 2020-10-02 青岛农业大学 一种表达荧光素酶的重组犬麻疹病毒
CN114058607A (zh) * 2020-07-31 2022-02-18 上海科技大学 一种用于c到u碱基编辑的融合蛋白及其制备方法和应用
CN114058607B (zh) * 2020-07-31 2024-02-27 上海科技大学 一种用于c到u碱基编辑的融合蛋白及其制备方法和应用
WO2022037666A1 (zh) * 2020-08-21 2022-02-24 北京大学 Rdr蛋白在肿瘤治疗中的应用
EP4263825A1 (en) * 2021-02-19 2023-10-25 Beam Therapeutics, Inc. Recombinant rabies viruses for gene therapy
CN112852817A (zh) * 2021-03-04 2021-05-28 中国人民解放军陆军特色医学中心 一种用于III-A型CRISPR-Csm系统中RNA编辑的组合物及应用
CN112852817B (zh) * 2021-03-04 2023-01-17 西部(重庆)科学城种质创制大科学中心 一种用于III-A型CRISPR-Csm系统中RNA编辑的组合物及应用

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